Version 1
February 2007
BioRobot® EZ1 DSP Workstation User Manual
9001360
9001360
1037181
1037181
QIAGEN
QIAGEN GmbH,
GmbH,D-40724
D-40724Hilden
Hilden
R1
Sample & Assay Technologies
Trademarks: QIAGEN®, BioRobot® (QIAGEN Group); Gigasept®, Lysetol®, Mikrozid® (Schülke & Mayr GmbH); DECON-QUAT® (Veltek Associates, Inc.).
© 2007 QIAGEN, all rights reserved.
Contents
Contents
1
Safety Information
1-1
1.1
Proper use
1-2
1.2
Electrical safety
1-2
1.3
Environment
1-4
1.4
Biological safety
1-5
1.5
Chemicals
1-6
1.6
Waste disposal
1-6
1.7
Mechanical hazards
1-7
1.8
Heat hazard
1-7
1.9
Symbols on the BioRobot EZ1 DSP
1-8
1.10
Additional symbols in this user manual
1-9
2
Introduction
2-1
2.1
About this user manual
2-1
2.2
2.2.1
2.2.2
2.2.3
General information
Technical assistance
Policy statement
Version management
2-1
2-1
2-2
2-2
2.3
2.3.1
Intended use of the BioRobot EZ1 DSP
Requirements for BioRobot EZ1 DSP users
2-2
2-3
3
3.1
3.1.1
3.1.2
3.1.3
3.1.4
3.1.5
General Description
External features of the BioRobot EZ1 DSP
Workstation door
Control panel
EZ1 Card slot
Status LEDs
Connector panel
BioRobot EZ1 DSP Workstation User Manual 02/2007
3-1
3-2
3-2
3-2
3-3
3-4
3-5
Contents-1
Contents
3.2
3.2.1
3.2.2
4
Internal features of the BioRobot EZ1 DSP
Worktable
Pipettor head
Installation Procedures
3-5
3-6
3-9
4-1
4.1
Requirements
4-1
4.2
AC power connection
4-1
4.3
Hardware installation
4-3
5
General Operation
5-1
5.1
Overview
5-1
5.2
5.2.1
5.2.2
Inserting and removing the EZ1 Card
Inserting the EZ1 Card
Removing the EZ1 Card
5-2
5-2
5-3
5.3
5.3.1
5.3.2
Switching the BioRobot EZ1 DSP on and off
Switching on the BioRobot EZ1 DSP
Switching off the BioRobot EZ1 DSP
5-4
5-4
5-5
5.4
5.4.1
5.4.2
Opening and closing the workstation door
Opening the workstation door
Closing the workstation door
5-5
5-6
5-6
5.5
5.5.1
5.5.2
Starting and stopping a protocol run
Starting a protocol run
Stopping a protocol run
5-6
5-6
5-7
5.6
5.6.1
5.6.2
5.6.3
Setting up the worktable
5-8
Removing and replacing the tray
5-9
Loading the reagent cartridges
5-9
Loading the elution tubes, filter-tips, and sample
tubes
5-11
6
Preventive Maintenance
6-1
6.1
Regular maintenance procedure
6-2
6.2
Daily maintenance procedure
6-4
Contents-2
BioRobot EZ1 DSP Workstation User Manual 02/2007
Contents
6.3
Weekly maintenance procedure
6-5
6.4
Reagents for decontamination
6-6
7
Troubleshooting
7-1
7.1
Detecting an error
7-1
7.2
Error codes
7-2
8
Glossary
8-1
Appendix A
A-1
Technical data
A-1
Waste Electrical and Electronic Equipment (WEEE)
A-4
Appendix B
B-1
Warranty statement
B-1
Liability clause
B-2
Index
BioRobot EZ1 DSP Workstation User Manual 02/2007
Index-1
Contents-3
Contents
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Contents-4
BioRobot EZ1 DSP Workstation User Manual 02/2007
Safety Information
1
Safety Information
This manual contains information and warnings that must be
followed by the user to ensure safe operation of the
BioRobot EZ1 DSP workstation and to maintain the
instrument in a safe condition.
Possible hazards that could harm the user or result in
damage to the instrument are clearly stated at the
appropriate places throughout this manual.
The following safety conventions are used throughout this
manual.
WARNING
The term WARNING is used to inform you about
situations that could result in personal injury to you or
other persons.
Details about these circumstances are given in a box like
this one.
CAUTION
The term CAUTION is used to inform you about
situations that could result in damage to the
instrument or other equipment.
Details about these circumstances are given in a box like
this one.
Before using the instrument, it is essential to read this
manual carefully and to pay particular attention to any
advice it contains concerning hazards that may arise from
use of the instrument.
The advice given in this manual is intended to supplement,
not supersede, the normal safety requirements prevailing in
the user’s country.
BioRobot EZ1 DSP Workstation User Manual 02/2007
1-1
Safety Information
1.1
WARNING
Proper use
Risk of personal injury and material damage
[W1]
Improper use of the BioRobot EZ1 DSP may cause
personal injuries or damage to the instrument.
The BioRobot EZ1 DSP must only be operated by
qualified personnel who have been appropriately
trained.
Servicing of the BioRobot EZ1 DSP must only be
performed by QIAGEN Instrument Service Specialists.
Use only QIAGEN® components, otherwise your right to
make a claim under the guarantee may be invalidated.
Carry out the maintenance regularly in accordance with the
operating instructions. QIAGEN charges for repairs that
prove to be required due to incorrect maintenance.
CAUTION
Damage to the instrument
[C1]
Avoid spilling water or chemicals onto the BioRobot EZ1
DSP. Instrument damage caused by water or chemical
spillage will void your warranty.
In case of an emergency, switch off the BioRobot EZ1 DSP at
the power switch on the rear of the instrument and unplug
the power cord from the wall power outlet.
CAUTION
1.2
Damage to the instrument
[C2]
Ensure that the BioRobot EZ1 DSP is switched off before
you manually move the mechanical components of the
instrument.
Electrical safety
To ensure satisfactory and safe operation of the
BioRobot EZ1 DSP, it is essential that the line power cord is
connected to true electrical earth (ground).
1-2
BioRobot EZ1 DSP Workstation User Manual 02/2007
Safety Information
WARNING
Electrical hazard
[W2]
Any interruption of the protective conductor
(earth/ground lead) inside or outside the instrument or
disconnection of the protective conductor terminal is
likely to make the instrument dangerous.
Intentional interruption is prohibited.
Lethal voltages inside the instrument
When the instrument is connected to line power,
terminals may be live, and opening covers or removing
parts is likely to expose live parts.
When working with the BioRobot EZ1 DSP:
„ Make sure the line power cord is connected to a line
power outlet that has a protective conductor (earth/
ground).
„ Do not attempt to make any internal adjustments or
replacements.
„ Do not operate the instrument with any covers or parts
removed.
„ If water or reagent has spilled inside the instrument,
switch off the instrument and disconnect it from the line
power supply. Call QIAGEN Technical Services.
„ Servicing should be carried out only by QIAGEN
Instrument Service Specialists.
„ If the instrument becomes electrically unsafe for use,
make the instrument inoperative and secure it against
unauthorized or unintentional operation. Call QIAGEN
Technical Services.
The instrument is likely to be electrically unsafe when:
„ It shows visible damage
„ The line power cord shows signs of damage
„ It has been stored under unfavorable conditions for a
prolonged period
„ It has been subjected to severe transport stresses
WARNING
Risk of electric shock
Do not touch the BioRobot EZ1 DSP with wet hands.
BioRobot EZ1 DSP Workstation User Manual 02/2007
[W3]
1-3
Safety Information
Voltage rating label
The following label appears on the back of the BioRobot EZ1
DSP. Ensure that the voltage rating specified on the label
matches the voltage available at the installation site.
1.3
Environment
Operating conditions
WARNING
Explosive atmosphere
The BioRobot EZ1 DSP is not designed for use in an
explosive atmosphere.
[W4]
The BioRobot EZ1 DSP will operate correctly under the
following conditions:
„ Indoors
„ Ambient temperature of 5–40ºC (41–104ºF)
„ Ambient relative humidity of <80% at <31ºC (88ºF) (no
condensation)
Storage conditions
If you intend to store the instrument for a prolonged period
of time, unplug the power cord from the wall power outlet.
The instrument can be stored at –25ºC to 70ºC (–13ºF to
158ºF).
1-4
BioRobot EZ1 DSP Workstation User Manual 02/2007
Safety Information
1.4
Biological safety
Specimens and reagents containing materials from humans
should be treated as potentially infectious. Use safe
laboratory procedures as outlined in publications such as
Biosafety in Microbiological and Biomedical Laboratories,
HHS (www.cdc.gov/od/ohs/biosfty/biosfty.htm).
Samples
Samples may contain infectious agents. You should be aware
of the health hazard presented by such agents and should
use, store, and dispose of such samples in accordance with
the required safety regulations.
WARNING
Samples containing infectious agents
[W5]
Some samples used with this instrument may contain
infectious agents. Handle such samples with the greatest
of care and in accordance with the required safety
regulations.
Always wear safety glasses, two pairs of gloves, and a
lab coat.
The responsible body (e.g., laboratory manager) must
take the necessary precautions to ensure that the
surrounding workplace is safe, and that the instrument
operators are suitably trained and not exposed to
hazardous levels of infectious agents as defined in the
applicable Material Safety Data Sheets (MSDSs) or
OSHA, ACGIH, or COSHH documents.
Venting for fumes and disposal of wastes must be in
accordance with all national, state, and local health and
safety regulations and laws.
OSHA:
Occupational Safety and Health Administration (United States of America).
ACGIH:
American Conference of Government Industrial Hygienists (United States of America).
COSHH: Control of Substances Hazardous to Health (United Kingdom).
BioRobot EZ1 DSP Workstation User Manual 02/2007
1-5
Safety Information
1.5
Chemicals
WARNING
Hazardous chemicals
[W6]
Some chemicals used with this instrument may be
hazardous or may become hazardous after completion
of the protocol run.
Always wear safety glasses, gloves, and a lab coat.
The responsible body (e.g., laboratory manager) must
take the necessary precautions to ensure that the
surrounding workplace is safe and that the instrument
operators are not exposed to hazardous levels of toxic
substances (chemical or biological) as defined in the
applicable Material Safety Data Sheets (MSDSs) or
OSHA, ACGIH, or COSHH documents.
Venting for fumes and disposal of wastes must be in
accordance with all national, state, and local health and
safety regulations and laws.
OSHA:
Occupational Safety and Health Administration (United States of America).
ACGIH:
American Conference of Government Industrial Hygienists (United States of America).
COSHH: Control of Substances Hazardous to Health (United Kingdom).
Toxic fumes
If you work with volatile solvents, toxic substances, etc., you
must provide an efficient laboratory ventilation system to
remove vapors that may be produced.
1.6
Waste disposal
Used labware, such as reagent cartridges and filter-tips, may
contain hazardous chemicals or infectious agents from the
purification process. Such wastes must be collected and
disposed of properly in accordance with the local safety
regulations.
For disposal of waste electrical and electronic equipment
(WEEE), see page A-4.
1-6
BioRobot EZ1 DSP Workstation User Manual 02/2007
Safety Information
1.7
Mechanical hazards
The worktable of the BioRobot EZ1 DSP moves during
operation of the instrument.
WARNING
Moving parts
[W7]
Never open the workstation door while the BioRobot EZ1
DSP is operating.
WARNING
Risk of overheating
[W8]
To ensure proper ventilation, maintain a minimum
clearance of 15 cm and 30 cm at the rear and sides of
the BioRobot EZ1 DSP, respectively.
Slits and openings which ensure the ventilation of the
BioRobot EZ1 DSP must not be covered.
1.8
Heat hazard
The BioRobot EZ1 DSP worktable contains a heating system.
WARNING
Hot surface
[W9]
The heating system can reach high temperatures. Avoid
touching it when it is hot.
BioRobot EZ1 DSP Workstation User Manual 02/2007
1-7
Safety Information
1.9
Symbol
1-8
Symbols on the BioRobot EZ1 DSP
Location
Description
Heating
system
Heat hazard — the temperature of the heating
system can reach up to 95ºC
Near the
tip rack
Biohazard — the tip rack may be contaminated
with biohazardous material and must be
handled with gloves
Type plate
on the
back of
the
instrument
CE mark for Europe
Type plate
on the
back of
the
instrument
UL listing mark for Canada and the USA
Type plate
on the
back of
the
instrument
WEEE mark for Europe
Type plate
on the
back of
the
instrument
In vitro diagnostic medical device
BioRobot EZ1 DSP Workstation User Manual 02/2007
Safety Information
Symbol
SN
1.10
Symbol
Location
Description
Type plate
on the
back of
the
instrument
Serial number
Type plate
on the
back of
the
instrument
Legal manufacturer
Additional symbols in this user manual
Location
Description
Front
cover
Catalog number
Front
cover
Handbook
BioRobot EZ1 DSP Workstation User Manual 02/2007
1-9
Safety Information
Symbol on the Heating System
Symbol near the Tip Rack
1-10
BioRobot EZ1 DSP Workstation User Manual 02/2007
Introduction
2
Introduction
Thank you for choosing the QIAGEN BioRobot EZ1 DSP
workstation. We are confident it will become an integral part
of your laboratory.
Before using the instrument, it is essential to read this
manual carefully and to pay particular attention to any
advice it contains concerning hazards that may arise from
use of this instrument.
2.1
About this user manual
This manual provides information about the BioRobot EZ1
DSP. It guides you systematically through the following
sections:
1. Safety Information
2. Introduction
3. BioRobot EZ1 DSP — General Description
4. Installation Procedures
5. General Operation
6. Preventive Maintenance
7. Troubleshooting
8. Glossary
Appendices
The Appendices contain the following:
„ Contact information for technical assistance
„ Technical data
„ Warranty terms
2.2
General information
2.2.1
Technical assistance
At QIAGEN we pride ourselves on the quality and availability
of our technical support. Our Technical Service Departments
are staffed by experienced scientists with extensive practical
and theoretical expertise in molecular biology and the use of
BioRobot EZ1 DSP Workstation User Manual 02/2007
2-1
Introduction
QIAGEN products. If you have any questions regarding the
BioRobot EZ1 DSP or QIAGEN products in general, please
do not hesitate to contact us.
QIAGEN customers are a major source of information
regarding advanced or specialized uses of our products. This
information is helpful to other scientists as well as to the
researchers at QIAGEN. We therefore encourage you to
contact us if you have any suggestions about product
performance or new applications and techniques.
For technical assistance and more information please call
one of the QIAGEN Technical Service Departments or local
distributors (see back cover).
2.2.2
Policy statement
It is the policy of QIAGEN to improve products as new
techniques and components become available. QIAGEN
reserves the right to change specifications at any time.
In an effort to produce useful and appropriate
documentation, we appreciate your comments on this
publication. Please contact QIAGEN Technical Services.
2.2.3
Version management
This document is the BioRobot EZ1 DSP Workstation User
Manual version 1, revision R1.
2.3
Intended use of the BioRobot EZ1 DSP
The BioRobot EZ1 DSP workstation is designed to perform
automated purification of nucleic acids for in vitro diagnostic
purposes. The system is intended for use by professional
users, such as technicians and physicians trained in
molecular biological techniques and the operation of the
BioRobot EZ1 DSP. Any diagnostic results that are generated
using the sample preparation procedure in conjunction with
any downstream diagnostic assay must be interpreted in
conjunction with other clinical or laboratory findings.
2-2
BioRobot EZ1 DSP Workstation User Manual 02/2007
Introduction
2.3.1
Requirements for BioRobot EZ1 DSP users
This table covers the general level of competence and
training necessary for transportation, installation, use,
maintenance, and servicing of the BioRobot EZ1 DSP.
Type of task
Personnel
Training and experience
Transportation
No special
requirements
No special requirements
Installation
Laboratory
technicians or
equivalent
Appropriately trained and
experienced personnel
familiar with use of computers
and automation in general
Routine use
(running
protocols)
Laboratory
technicians or
equivalent
Appropriately trained and
experienced personnel
familiar with use of computers
and automation in general
Preventive
maintenance
Laboratory
technicians or
equivalent
Appropriately trained and
experienced personnel
familiar with use of computers
and automation in general
Servicing and
annual preventive
maintenance
QIAGEN
Instrument
Service
Specialists only
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2-3
Introduction
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2-4
BioRobot EZ1 DSP Workstation User Manual 02/2007
General Description
3
General Description
The BioRobot EZ1 DSP workstation performs fully automated
nucleic acid purification from up to 6 samples using
magnetic particles. The automated steps include:
„ Lysis of samples
„ Binding of nucleic acids to magnetic particles
„ Washing and elution of nucleic acids
The user inserts an EZ1 Card containing protocol(s) into the
BioRobot EZ1 DSP. After starting worktable setup using the
control panel, the user loads samples, reagent cartridges,
filter-tips in tip holders, and elution tubes onto the
BioRobot EZ1 DSP worktable. The user then closes the
workstation door and starts the protocol. The protocol
provides all the necessary instructions for the BioRobot EZ1
DSP to carry out automated nucleic acid purification.
The aspiration and dispensing of samples and reagents and
the separation of magnetic particles are performed by the
6-channel pipettor head. The temperature of samples is
regulated by a heating system.
BioRobot EZ1 DSP Workstation User Manual 02/2007
3-1
General Description
3.1
External features of the BioRobot EZ1 DSP
Front View of the BioRobot EZ1 DSP Workstation
1
5
2
3
4
3.1.1
1
Workstation door
4
EZ1 Card
2
Control panel
5
Status LEDs
3
EZ1 Card slot
Workstation door
The workstation door can be manually opened by the user in
order to gain access to the worktable. A protocol cannot be
started until the workstation door is closed.
3.1.2
Control panel
The control panel is the user interface that allows the user to
operate the BioRobot EZ1 DSP. It consists of a keypad and a
liquid-crystal display (LCD).
3-2
BioRobot EZ1 DSP Workstation User Manual 02/2007
General Description
Keypad
The following keys of the control panel are available to the
user.
Key
Description
0–9
Selects a menu.
START
Initiates an action or starts a protocol.
STOP
Interrupts a protocol run.
ESC
Displays the previous menu or text.
The other keys of the control panel are for service personnel
only.
Liquid-crystal display
The LCD consists of 4 lines. There are 20 spaces per line.
Liquid-Crystal Display
3.1.3
EZ1 Card slot
The EZ1 Card slot accepts an EZ1 Card. Each EZ1 Card
contains one or more protocols that allow the BioRobot EZ1
DSP to perform nucleic acid isolation.
Note: The workstation should only be switched on after an
EZ1 Card is inserted. Make sure that the EZ1 Card is
completely inserted! Otherwise essential instrument data
BioRobot EZ1 DSP Workstation User Manual 02/2007
3-3
General Description
could be lost, leading to a memory error. EZ1 Cards should
not be exchanged while the workstation is switched on. Do
not remove the EZ1 Card while the machine is switched on.
Also, take care not to expose the EZ1 Card to electric shock,
water, or dirt.
EZ1 Card Completely Inserted into EZ1 Card Slot
3.1.4
Status LEDs
There are 2 light-emitting diodes (LEDs) at the front of the
BioRobot EZ1 DSP:
„ Green LED — indicates that the instrument is receiving
power
„ Red LED — indicates that an error has occurred
3-4
BioRobot EZ1 DSP Workstation User Manual 02/2007
General Description
3.1.5
Connector panel
The connector panel is located at the rear of the BioRobot
EZ1 DSP.
Connector Panel
Power switch
Power cord
Fuse box
Label indicating
the correct fuses
for the fuse box
Connector for
computer cable
It contains the power switch, the socket for the power cord,
the fuse box, and a connector for a computer cable (for
service use only).
3.2
Internal features of the BioRobot EZ1 DSP
The interior of the BioRobot EZ1 DSP contains the worktable
and the pipettor head.
BioRobot EZ1 DSP Workstation User Manual 02/2007
3-5
General Description
3.2.1
Worktable
The worktable contains various racks for holding the
labware, samples, and reagent cartridges that are required
for the protocol run. The worktable also contains a heating
system for controlling the temperature of samples.
The BioRobot EZ1 DSP Worktable
Pipettor head
Heating system
Sample tubes
Reagent
cartridges in
cartridge rack
Sample tubes
Tip holders with
tips in tip rack
Elution tubes
Tip rack
This rack is located in the front of the worktable. The front
row holds up to 1.5 ml elution tubes. The recommended
elution tubes are screw-capped, made of polypropylene, and
supplied by Sarstedt (cat. no. 72.692).
The next 2 rows hold up to 12 tip holders containing filtertips. (Note: Some protocols require only one row of filtertips.) The remaining row holds up to 6 sample tubes. Sample
tubes are 2 ml in volume, screw-capped, made of
polypropylene, and available from Sarstedt (cat. no.
72.693).
3-6
BioRobot EZ1 DSP Workstation User Manual 02/2007
General Description
Tip Rack
Both tip holders and filter-tips are made of polypropylene
and available from QIAGEN. The filter-tips have a capacity
of 50–1000 μl.
Tip Holders and Filter-tips
BioRobot EZ1 DSP Workstation User Manual 02/2007
3-7
General Description
Cartridge rack
This rack is located behind the tip rack and holds up to
6 reagent cartridges.
Cartridge Rack
Reagent cartridges are made of polypropylene, contain
prefilled reagents, and are available from QIAGEN.
Reagent Cartridges
Heating
positions
Reagent wells
A reagent cartridge consists of 10 reagent wells and
2 heating positions. One heating position is a well, the other
is a slot that can accept a tube.
3-8
BioRobot EZ1 DSP Workstation User Manual 02/2007
General Description
Heating system
The heating system is located under the far end of the
cartridge rack. It holds the heating positions of the reagent
cartridges.
Tray
The tray is located under the racks and collects any drops of
liquid that may fall.
Tray
3.2.2
Pipettor head
The pipettor head is mounted above the worktable and
moves in the Z direction (i.e., up and down) in order to reach
the samples and reagents on the worktable. The worktable
itself moves in the Y direction (i.e., front to back) in order to
present different samples and reagents under the pipettor
head.
The pipettor head contains 6 high-precision syringe pumps
that are connected to tip adapters that can be attached to
filter-tips. The syringe pumps operate simultaneously to allow
aspiration or dispensing of small volumes of liquid
(50–1000 μl) via the filter-tips.
The pipettor head also contains a magnet whose distance
from the attached filter-tips can be varied. This feature allows
the capture of magnetic particles that are present in the
liquid aspirated into the filter-tips.
Located behind the tip adapters is the piercing unit, a row of
6 metal spikes for puncturing the foil that covers the reagent
cartridges.
BioRobot EZ1 DSP Workstation User Manual 02/2007
3-9
General Description
During operation of the BioRobot EZ1 DSP, the piercing unit
first exposes the reagents in the reagent cartridges. The
pipettor head then picks up filter-tips from the tip rack, and
performs aspiration and dispensing operations at different
locations on the worktable before ejecting the tips back into
the tip rack.
3-10
BioRobot EZ1 DSP Workstation User Manual 02/2007
Installation Procedures
4
Installation Procedures
The BioRobot EZ1 DSP is a plug-and-play instrument,
making unpacking and installation easy. A member of your
group who is familiar with laboratory equipment should
oversee the installation.
4.1
Requirements
Site
The BioRobot EZ1 DSP must be located out of direct sunlight,
away from heat sources, and away from sources of vibration
and electrical interference. Refer to Appendix A for the
operating conditions (temperature and humidity).
Use a level workbench that is large and strong enough to
accommodate the BioRobot EZ1 DSP. Refer to Appendix A
for the weight and dimensions of the BioRobot EZ1 DSP.
The BioRobot EZ1 DSP must be placed near to a properly
grounded (earthed) AC power outlet. The power line to the
instrument should be voltage regulated and surge protected.
4.2
AC power connection
Power requirements
The BioRobot EZ1 DSP operates at:
„ 200–240 V AC ± 10%, 50/60 Hz, 300 VA
„ 100–120 V AC ± 10%, 50/60 Hz, 300 VA
Make sure the voltage rating of the BioRobot EZ1 DSP is
compatible with the AC voltage available at the installation
site.
Grounding requirements
To protect operating personnel, the BioRobot EZ1 DSP must
be correctly grounded (earthed). The workstation is equipped
with a 3-conductor AC power cord that, when connected to
an appropriate AC power outlet, grounds (earths) the
BioRobot EZ1 DSP Workstation User Manual 02/2007
4-1
Installation Procedures
workstation. To preserve this protection feature, do not
operate the workstation from an AC power outlet that has no
ground (earth) connection.
Installation of AC power cord
Connect one end of the AC power cord to the socket located
on the rear of the BioRobot EZ1 DSP, and the other end to
the AC power outlet.
The fuse box of the BioRobot EZ1 DSP is located under the
socket for the power cord and contains 2 fuses which are
labeled as follows:
„ 110–120 V — a 6.3 A (250 V) fuse compatible with
110–120 V power supplies
„ 220–240 V — a 3.15 A (250 V) fuse compatible with
220–240 V power supplies
Before connecting the instrument to the power supply, you
may need to rotate the fuse box in order to select the correct
fuse. A fuse is correctly selected when its label is readable
and closer to the bottom of the instrument.
Selecting the Correct Fuse for a
110–120 V Power Supply
4-2
BioRobot EZ1 DSP Workstation User Manual 02/2007
Installation Procedures
WARNING
4.3
Electrical hazard
[W12]
Never install a fuse different from that specified in the
user manual.
Hardware installation
The following items are delivered:
„ BioRobot EZ1 DSP workstation
„ Power cord (8 pieces)
„ BioRobot EZ1 DSP accessories
„ BioRobot EZ1 DSP Workstation User Manual
„ 6GC teaching data sheet
The BioRobot EZ1 DSP accessories include:
„ Cartridge rack
„ Tip rack
„ Tray
„ O-ring (pack of 6) and silicon grease
„ Fuses (1 each: 6.3 A and 3.15 A)
Unpacking the BioRobot EZ1 DSP
1. Cut and remove the plastic straps.
Plastic straps
BioRobot EZ1 DSP Workstation User Manual 02/2007
4-3
Installation Procedures
2. Remove the outer top cardboard.
Outer top
cardboard
Disposables
and
accessories
Cardboard
tray
Corner shock
absorbers
Outer
cardboard
box
Wrapped
BioRobot EZ1
DSP
Aluminum
strap
Corner shock
absorbers
Outer bottom
cardboard
3. Take out the packages with the disposables and
accessories.
4. Remove the cardboard tray.
5. Pull out the 4 corner shock absorbers at the top, and
remove the outer cardboard box by pulling it up.
6. Take out the wrapped BioRobot EZ1 DSP, and remove
the aluminum strap.
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Installation Procedures
7. Remove the inner top cardboard, and remove the
BioRobot EZ1 DSP from the inner cardboard box.
Inner top
cardboard
BioRobot EZ1
DSP
Inner
cardboard
box
8. Remove all plastic sheets.
BioRobot EZ1
DSP
Plastic sheets
Inner bottom
cardboard
Note: Be careful not to damage the surface of the BioRobot
EZ1 DSP after removing the plastic sheets.
Note: It is recommended to save the original packaging
material for later transportation of the BioRobot EZ1 DSP.
Removing the protectors for the Y- and Z-axes
During transportation, protectors prevent the moveable parts
of the BioRobot EZ1 DSP from moving along the Y- and
Z-axes. Before using the instrument, these protectors must be
removed.
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Installation Procedures
1. Cut and remove the cable tie.
Silica gel
Cable tie
Bubble wrap
2. Remove the bubble wrap and the silica gel.
3. Push the pipettor head upward.
Pipettor head
4. Remove the bag of foam packaging material.
Bag
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BioRobot EZ1 DSP Workstation User Manual 02/2007
Installation Procedures
Note: It is recommended to save the original packaging
material for later transportation of the BioRobot EZ1 DSP.
Installing the BioRobot EZ1 DSP
1. Adjust the fuse box if necessary (Section 4.2, page 4-1).
2. Connect the BioRobot EZ1 DSP to the power cord.
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Installation Procedures
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BioRobot EZ1 DSP Workstation User Manual 02/2007
General Operation
5
General Operation
This section describes how to operate the BioRobot EZ1 DSP.
Before proceeding, it is recommended that you familiarize
yourself with the features of the BioRobot EZ1 DSP by
referring to Section 3.
5.1
Overview
The steps for operating the BioRobot EZ1 DSP are presented
below. Further details are provided later in this section.
1. Insert the EZ1 Card completely into the EZ1 Card slot.
2. Switch on the BioRobot EZ1 DSP.
3. Press “START” to start worktable setup, and press any key
to proceed through the messages displayed by the LCD.
4. Open the workstation door.
5. Set up the worktable according to the messages
displayed by the LCD:
„ Load the reagent cartridges into the cartridge rack;
then load the rack onto the worktable
„ Load elution tubes into the tip rack
„ Load filter-tips and sample tubes into the tip rack;
then load the rack onto the worktable
6. Close the workstation door.
7. Press “START” to start the protocol.
8. At the end of the protocol run:
„ Press “STOP” twice if you do not want to run another
protocol
„ Press “ESC” if you want to run another protocol
9. Remove the purified nucleic acid samples.
10. Clean the BioRobot EZ1 DSP.
11. Run another protocol or switch off the BioRobot EZ1 DSP.
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5-1
General Operation
5.2
CAUTION
5.2.1
Inserting and removing the EZ1 Card
Damage to the instrument
[C3]
Ensure that the BioRobot EZ1 DSP is switched off before
you insert or remove the EZ1 Card. Otherwise, the card
and/or the instrument may be damaged.
Inserting the EZ1 Card
1. Lift up the cover of the EZ1 Card slot.
2. Insert the EZ1 Card into the EZ1 Card slot.
Orientate the card so that the picture faces to the left and the
triangle symbol is at the bottom.
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General Operation
Ensure that the card is completely inserted into the slot.
It is extremely important that the EZ1 Card is completely
inserted to avoid memory error and loss of essential
instrument data.
3. Close the cover of the EZ1 Card slot.
4. Switch on the BioRobot EZ1 DSP (Section 5.3.1, page
5-4).
Note: Do not remove the EZ1 Card while the machine is
switched on.
5.2.2
Removing the EZ1 Card
1. Switch off the BioRobot EZ1 DSP (Section 5.3.2, page
5-5).
2. Lift up the cover of the EZ1 Card slot.
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5-3
General Operation
3. Press the button at the bottom of the EZ1 Card slot.
The EZ1 Card is ejected.
5.3
Switching the BioRobot EZ1 DSP on and
off
5.3.1
Switching on the BioRobot EZ1 DSP
1. Insert the EZ1 Card (Section 5.2.1, page 5-2).
2. Switch on the BioRobot EZ1 DSP at the rear power switch.
3. The LCD displays the following text for a few seconds.
This text indicates that the BioRobot EZ1 DSP is
initializing. All motors move to their home positions.
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General Operation
4. After initialization, the main menu appears.
You can now operate the BioRobot EZ1 DSP.
5.3.2
Switching off the BioRobot EZ1 DSP
1. If you had run a protocol, refer to “End of the protocol
run” on page 5-7.
2. Switch off the BioRobot EZ1 DSP at the rear power
switch.
5.4
WARNING
Opening and closing the workstation
door
Moving parts
[W7]
Never open the workstation door while the BioRobot EZ1
DSP is operating.
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5-5
General Operation
5.4.1
Opening the workstation door
1. Slide your fingers under the door and push it upward.
2. Rest the door on the door lock that is located near the
top of the BioRobot EZ1 DSP.
5.4.2
Closing the workstation door
1. Press the door down to disengage the door lock.
2. Gradually lower the door until it rests on the worktable.
Take care that your fingers do not get caught between
the door and worktable.
5.5
WARNING
5.5.1
Starting and stopping a protocol run
Moving parts
[W7]
Never open the workstation door while the BioRobot EZ1
DSP is operating.
Starting a protocol run
After you have inserted the EZ1 Card and switched on the
BioRobot EZ1 DSP, start a protocol as follows:
1. Press “START” on the control panel.
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General Operation
2. Start worktable setup by pressing the appropriate
number key (e.g., “1” or “2”).
For details, refer to the handbook supplied with the EZ1
DSP Kit you are using.
3. Follow the instructions displayed by the LCD.
4. Open the workstation door and set up the worktable
according to the messages displayed by the LCD
(Section 5.6, page 5-8).
5. Close the workstation door.
The protocol run cannot start until the door is closed.
6. Press “START” to start the protocol run.
End of the protocol run
After a protocol run ends, the LCD displays “Finished”:
1. Press “STOP” twice if you are not running another
protocol. The LCD displays the main menu and the
motors return to their home positions.
Alternatively, press “ESC” if you are running another
protocol. The LCD displays “Protocols”, from which you
can start the next worktable setup.
2. Check that the BioRobot EZ1 DSP is no longer operating.
Then open the workstation door and remove the elution
tubes, which contain the purified nucleic acid samples.
3. Clean the workstation (Section 6, page 6-1).
4. Run another protocol or switch off the BioRobot EZ1 DSP.
5.5.2
Stopping a protocol run
When a protocol is running, you have the option of either
pausing or canceling it:
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5-7
General Operation
1. Press “STOP”. The protocol run pauses and the LCD
displays the following text.
2. To continue the protocol run, press “START”.
The protocol run continues from where it had stopped.
3. To cancel the protocol run, press “STOP”. The LCD
displays the main menu.
Then follow these steps:
„ Ensure that the workstation door is closed
„ Press “1” to display “Manual” in the LCD
„
„
„
5.6
Press “2” to return the tips to the tip rack and to
return the motors to their home positions
When “Manual” reappears in the LCD, press “ESC”
to redisplay the main menu
Another protocol can now be run
Setting up the worktable
Note: For specific details about setting up the worktable,
refer to the onscreen instructions, which are also included in
the handbook supplied with the EZ1 DSP Kit you are using.
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BioRobot EZ1 DSP Workstation User Manual 02/2007
General Operation
5.6.1
Removing and replacing the tray
The tray is located under the worktable and can be removed
for cleaning:
1. Switch off the BioRobot EZ1 DSP.
2. Push the worktable toward the back of the BioRobot EZ1
DSP.
3. Remove the tray using its handle.
Reverse this procedure to replace the tray. To avoid
instrument damage, ensure that the tray is positioned
correctly.
5.6.2
Loading the reagent cartridges
Note: Do not remove the foil from the reagent cartridges.
1. Invert the reagent cartridges several times to mix the
magnetic particles.
2. Tap the reagent cartridges until the reagents are
deposited at the bottom of the wells.
3. Remove the cartridge rack from the worktable.
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General Operation
4. Slide the reagent cartridges into the cartridge rack in the
direction of the arrow, as shown below, until you feel
resistance.
Press down the cartridges until they click into place.
5. Return the cartridge rack to the worktable.
Ensure that the 2 heating positions of each cartridge fit
into the heating system.
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General Operation
5.6.3
Loading the elution tubes, filter-tips, and sample
tubes
1. Remove the tip rack from the worktable.
2. Load the elution tubes into row 1 of the tip rack.
Label the elution tubes before you load them into the
rack. Ensure that the caps of the tubes are removed
before you start the protocol.
3. Place the filter-tips into the tip holders.
Ensure that the tips are positioned correctly in the
holders.
4. Load the tip holders into rows 2 and 3 of the tip rack.
Note: Some protocols require only one row of filter-tips.
Please follow the messages displayed by the LCD.
5. Load the sample tubes into row 4 of the tip rack.
Ensure that the caps of the tubes are removed before you
start the protocol.
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General Operation
6. Return the tip rack to the worktable.
Ensure that the tips, tip holders, and tip rack are correctly
positioned on the worktable.
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BioRobot EZ1 DSP Workstation User Manual 02/2007
Preventive Maintenance
6
Preventive Maintenance
The following preventive maintenance procedures must be
carried out to ensure reliable operation of the BioRobot EZ1
DSP:
„ Regular preventive maintenance — after each
protocol run
„ Daily preventive maintenance — after the last
protocol run of the day
„ Weekly preventive maintenance — every week
„ Annual preventive maintenance — every year;
removal and replacement of parts are carried out by
QIAGEN Instrument Service Specialists only
WARNING
Risk of electric shock
Do not open any panels on the BioRobot EZ1 DSP.
Risk of personal injury and material damage
Only perform maintenance which is specifically
described in this manual.
[W10]
Servicing
Each BioRobot EZ1 DSP workstation is supplied with a oneyear warranty that includes all repairs due to mechanical
breakdown. Worldwide, the maximum time for response to a
breakdown is 5 days. Application development, software
upgrades, worktable accessories, disposable items, and
replacement of spare parts such as syringes, tubing, and
pipet tips are not included in the warranty.
QIAGEN offers comprehensive Service Support Agreements,
including IQ/OQ, Warranty Extensions, Full Cover Support
Agreements, and Preventive Maintenance Agreements.
Service Support Agreements ensure high performance from
your workstation. In addition, service histories are fully
documented and all parts are certified and guaranteed.
Contact your local QIAGEN Instrument Service
representative, or your local distributor for more information
about flexible Service Support Agreements from QIAGEN.
BioRobot EZ1 DSP Workstation User Manual 02/2007
6-1
Preventive Maintenance
6.1
Regular maintenance procedure
WARNING
Risk of personal injury and material damage
[W1]
Improper use of the BioRobot EZ1 DSP may cause
personal injuries or damage to the instrument.
The BioRobot EZ1 DSP must only be operated by
qualified personnel who have been appropriately
trained.
Servicing of the BioRobot EZ1 DSP must only be
performed by QIAGEN Instrument Service Specialists.
WARNING
Hazardous chemicals and infectious agents
[W11]
The waste contains samples and reagents. This waste
may contain toxic or infectious material and must be
disposed of properly. Refer to your local safety
regulations for proper disposal procedures.
If working with potentially infectious materials, such as
human blood, serum, or plasma, the BioRobot EZ1 DSP
system should be decontaminated after use (Section 6.4,
page 6-6).
After running a protocol, clean the piercing unit of the
pipettor head:
1. Remove used disposable labware and unwanted samples
and reagents. Discard them according to your local
safety regulations.
2. In the main menu, press “1” to display “Tools”.
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BioRobot EZ1 DSP Workstation User Manual 02/2007
Preventive Maintenance
3. Press “3” to select “Clean pierce unit”.
The piercing unit moves downward.
4. Wipe the piercing unit using a soft tissue moistened with
70% ethanol. The piercing unit is sharp. Double gloves
are recommended.
Piercing unit
5. Wipe the piercing unit using a soft tissue moistened with
distilled water.
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6-3
Preventive Maintenance
6. Press “ESC” to return the piercing unit to its original
position.
7. Clean the tray and racks with 70% ethanol and then with
distilled water.
8. Clean the BioRobot EZ1 DSP worktable with 70% ethanol
and then with distilled water.
9. Wipe the other surfaces of the workatable with a diluted
neutral soap solution and then with distilled water.
You can now run another protocol or switch off the
BioRobot EZ1 DSP.
6.2
WARNING
Daily maintenance procedure
Hazardous chemicals and infectious agents
[W11]
The waste contains samples and reagents. This waste
may contain toxic or infectious material and must be
disposed of properly. Refer to your local safety
regulations for proper disposal procedures.
After running the last protocol of the day, perform the daily
maintenance procedure:
1. Clean the piercing unit (Section 6.1, page 6-2).
2. Remove used disposable labware and unwanted samples
and reagents, and discard them according to your local
safety regulations.
3. Check that the tray is clean. If necessary, clean it with
70% ethanol, and then with distilled water.
4. Clean the worktable and its racks with 70% ethanol, and
then with distilled water.
5. Wipe the other surfaces of the workstation with diluted
neutral soap solution, and then with water.
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Preventive Maintenance
6. Wipe the O-rings of the tip adapters with tissue.
6.3
Weekly maintenance procedure
Perform the daily maintenance procedure before you
perform the weekly maintenance procedure.
To maintain good contact between tip adapters and filter-tips
and to prevent liquid leaking from the tips, grease the
O-rings of the tip adapters every week:
1. Apply a small amount of silicon grease to the end of a
filter-tip.
2. Apply the silicon grease to the surface of the O-rings.
3. Place the tip onto the pipettor head, and rotate the tip on
the pipettor head to distribute the silicon grease evenly.
O-ring
Gap
Note: The filter-tips should sit flush against the upper
white plastic bar if the O-rings are properly greased.
There should not be a gap. Excess or insufficient grease
can affect the performance of the BioRobot EZ1 DSP.
BioRobot EZ1 DSP Workstation User Manual 02/2007
6-5
Preventive Maintenance
6.4
Reagents for decontamination
The following disinfectants and detergents are compatible
with surfaces and components of the BioRobot EZ1 DSP
system. Use according to manufacturers’ instructions for
effective disinfection.
WARNING
Toxic fumes
[W13]
Do not use bleach to clean or disinfect the instrument.
Bleach in contact with salts from the buffers can produce
toxic fumes.
„ Mikrozid® Liquid (Schülke & Mayr GmbH;
www.schuelke-mayr.com )* — ethanol-based disinfectant
for cleaning surfaces, such as the worktable (consists of
25 g ethanol and 35 g 1-propanol per 100 g Mikrozid
Liquid)
„ Lysetol® AF or Gigasept® Instru AF (Schülke & Mayr
GmbH)* — quaternary ammonium solution for
submerging worktable items, such as holders (consists of
14 g cocospropylene-diamine-guanidine diacetate, 35 g
phenoxypropanols, and 2.5 g benzalkonium chloride per
100 g, with anticorrosion components, fragrance, and
15–30% nonionic surfactants)
Note: If you want to use disinfectants different from those
recommended, ensure that their compositions are similar to
those described above. A suitable alternative to Mikrozid
Liquid is Incidin Liquid (EcoLab; www.ecolab.com ). A
suitable alternative to Lysetol AF or Gigasept Instru AF is
DECON-QUAT® 100 (Veltek Associates, Inc.;
www.sterile.com ).*
Note: Do not spray cleaning or disinfectant liquids onto
surfaces of the BioRobot EZ1 DSP. Spray bottles should be
used only for items that have been removed from the
workstation.
* This is not a complete list of suppliers and does not include
many important vendors of biological supplies.
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Troubleshooting
7
Troubleshooting
7.1
Detecting an error
If a protocol run is interrupted due to an error:
„ The red LED flashes
„ An alarm sounds
„ The LCD displays an error message
LCD Displaying an Error Message
The second line of the error message gives the error code
(see list below). The third line indicates the line number of the
protocol at which the error occurred.
Record the error code and the line number, and contact
QIAGEN Technical Services. Then reset the BioRobot EZ1
DSP:
1. Press “ESC” to display the main menu in the LCD.
2. Ensure that the workstation door is closed.
3. Press “1” to display “Manual” in the LCD.
4. Press “2” to return the tips to the tip rack and to return
the motors to their home positions.
5. When “Manual” reappears in the LCD, press “ESC” to
redisplay the main menu.
Another protocol can now be run.
Note: It is not possible to continue a protocol run that has
been interrupted due to an error.
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7-1
Troubleshooting
7.2
Error codes
Error code
Description
10
Failed to return to origins, protocol cannot run
11
Limit error, protocol cannot run
12
Failed to return to Z axis, protocol in run
13
Failed to return to P axis, protocol in run
14
Failed to return to M axis, protocol in run
15
Failed to return to Y axis, protocol in run
16
Z axis limit error, protocol in run
19
Y axis end limit, protocol in run
20
Z axis time-out, protocol in run
21
P axis time-out, protocol in run
22
M axis time-out, protocol in run
23
Y axis time-out, protocol in run
24
Open door in motion
25
Abnormal input from bottom sensor in motion
26
Failed to initialize heating block
27
Failed to initialize motion control board
29
Memory error
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Glossary
8
Glossary
Term
Description
Cartridge
rack
A metal rack that accommodates reagent cartridges on the
worktable.
Connector
panel
The panel on the rear of the BioRobot EZ1 DSP. It contains
the power switch, the socket for the power cord, the fuse
box, and a connector for a computer cable.
Control
panel
The user interface that allows the user to operate the
BioRobot EZ1 DSP. The control panel consists of an LCD
and a keypad.
Elution tube
A polypropylene, screw-capped 1.5 ml tube for collecting
purified nucleic acids.
Error code
A 1 or 2 digit number that indicates a particular error of
the BioRobot EZ1 DSP.
EZ1 Card
A card that contains one or more protocols for the
BioRobot EZ1 DSP and is inserted into the instrument.
EZ1 Card
slot
A slot at the front of the BioRobot EZ1 DSP that accepts an
EZ1 Card.
Filter-tip
An item of labware that is picked up by a tip adapter
during operation of the BioRobot EZ1 DSP. Liquid is
aspirated into and dispensed from a filter-tip. A filter-tip is
also the location where separation of magnetic particles
occurs.
Heating
system
A component of the BioRobot EZ1 DSP that accommodates
the heating positions of the reagent cartridges and heats
samples.
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8-1
Glossary
Term
Description
O-ring
A ring that is fitted at the bottom of a tip adapter. It is
required for good contact between the tip adapter and a
filter-tip.
Pipettor
head
The component of the BioRobot EZ1 DSP that aspirates
and dispenses liquid and separates magnetic particles. The
pipettor head moves up and down above the worktable
and contains 6 syringe pumps, each of which is connected
to a tip adapter.
Protocol
A set of instructions for the BioRobot EZ1 DSP that allows
the instrument to automate a nucleic acid purification
procedure. Protocols are run using the control panel.
Reagent
cartridge
An item of labware that contains 10 wells and 2 heating
positions. One heating position is a well, the other is a slot
that can accept a tube. A reagent cartridge is prefilled with
reagents.
Sample tube
A polypropylene, screw-capped 2 ml tube for holding a
sample containing nucleic acids to be purified.
Tip adapter
One of 6 metal probes installed on the pipettor head.
During operation of the BioRobot EZ1 DSP, the tip
adapters pick up filter-tips from the worktable.
Tip holder
A polypropylene tube that holds a single filter-tip. Tip
holders are loaded onto the tip rack.
Tip rack
A metal rack that accommodates tip holders containing
filter-tips on the worktable. The tip rack also
accommodates sample tubes and elution tubes.
Tray
A metal tray is located under the worktable. It collects any
drops of liquid that may fall.
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Glossary
Term
Description
Workstation
door
The main door at the front of the BioRobot EZ1 DSP. When
open, it provides complete access to the worktable.
Worktable
The surface of the BioRobot EZ1 DSP that contains racks
and is where samples, reagent cartridges, and disposable
labware are loaded. The worktable moves backwards and
forwards to present different samples and reagents under
the pipettor head.
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Glossary
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8-4
BioRobot EZ1 DSP Workstation User Manual 02/2007
Appendix A
Appendix A
Technical data
Environmental conditions
Power
100–120/200–240 V AC ± 10%, 50/60 Hz, 300 VA
Fuses
6.3 A (250 V) (for 110–120 V AC)
3.15 A (250 V) (for 220–240 V AC)
WARNING
Electrical hazard
[W12]
Never install a fuse different from that specified in the
user manual.
Operating
temperature
5–40ºC (41–104ºF)
Humidity
Maximum 80% relative humidity for temperatures up to
31ºC (88ºF), decreasing linearly to 50% humidity at 40°C
(104°F) (no condensation)
Altitude
Up to 2000 m (6500 ft.)
Place of
operation
For indoor use only
Storage
temperature
–25ºC to 70ºC (–13ºF to 158ºF)
Pollution
level
2
Overvoltage
category
II
BioRobot EZ1 DSP Workstation User Manual 02/2007
A-1
Appendix A
Mechanical data and hardware features
Dimensions
Width: 0.32 m (13 in.)
Height: 0.54 m (21 in.) with door closed
0.73 m (29 in.) with door open
Depth: 0.50 m (20 in.)
Mass
25.8 kg (57 lb.)
Instrument
features
„ Automated nucleic acid isolation using magnetic
particles
„ Desktop instrument
„ Protocols stored on EZ1 Cards
„ Aspirates and dispenses 6 samples or reagents
simultaneously using a 6-channel pipettor head
„ Separates magnetic particles using patented
technology
„ Processes up to 6 samples in one run
„ Controlled through LCD user interface
„ Temperature control through a heating system
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Appendix A
Pipettor
head
Contains 6 high-precision syringe pumps, each containing
a tip adapter which attaches to filter-tips. Each tip aspirates
and dispenses 50–1000 μl liquid.
Pipetting accuracy is as follows:
„ 50–100 μl
± 5%
„ 100–1000 μl:
± 2%
Syringe pumps are air-filled.
Liquids containing salts, alcohol, solvents, and/or
magnetic particles can be aspirated and dispensed.
Air gaps can be aspirated to prevent aspirated liquid from
dripping.
The pipettor head contains a magnet that allows
separation of magnetic particles from the aspirated liquid.
Filter-tips are picked up from the tip rack and ejected back
into the tip rack.
The pipettor head moves in the Z direction above the
worktable.
Heating
system
Accommodates the heating positions of reagent cartridges
and has a temperature range of between ambient
temperature and 95ºC (203ºF).
Heating block accuracy at 60°C is ± 2°C.
Filter-tips
Attach to the tip adapters of the pipettor head to allow
liquid aspiration and dispensing. Capacity of 50–1000 μl.
The BioRobot EZ1 DSP accommodates up to 12 tip
holders, each containing a filter-tip, in the tip rack on the
worktable.
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A-3
Appendix A
Labware
Reagents are loaded onto the worktable using the reagent
cartridges. These cartridges are already prefilled with
reagents by QIAGEN.
Several reagent cartridges are accommodated together on
the worktable by a cartridge rack.
Samples are loaded onto the worktable using 2 ml sample
tubes.
Steps which require heating occur on the heating system,
which accommodates the heating positions of the reagent
cartridges.
Purified nucleic acids are collected in 1.5 ml elution tubes.
Waste Electrical and Electronic Equipment
(WEEE)
This section provides information about disposal of waste
electrical and electronic equipment by users in the European
Union.
The European Directive 2002/96/EC on WEEE requires
proper disposal of electrical and electronic equipment when
it reaches its end of life. The crossed-out wheeled bin symbol
(see below) indicates that this product must not be disposed
of with other waste; it must be taken to an approved
treatment facility or to a designated collection point for
recycling, according to local legislation. The separate
collection and recycling of waste electronic equipment at the
time of disposal helps to conserve natural resources and
ensures that the product is recycled in a manner that protects
human health and the environment.
A-4
BioRobot EZ1 DSP Workstation User Manual 02/2007
Appendix A
QIAGEN accepts its responsibility in accordance with the
specific WEEE recycling requirements and, where a
replacement product is being supplied by QIAGEN, provides
free recycling of its WEEE-marked electronic equipment in
Europe. If a replacement product is not being purchased
from QIAGEN, recycling can be provided upon request at
additional cost. To recycle electronic equipment, contact your
local QIAGEN sales office for the required return form. Once
the form is submitted, you will be contacted by QIAGEN
either to request follow-up information for scheduling
collection of the electronic waste or to provide you with an
individual quote.
BioRobot EZ1 DSP Workstation User Manual 02/2007
A-5
Appendix A
This page intentionally left blank
A-6
BioRobot EZ1 DSP Workstation User Manual 02/2007
Appendix B
Appendix B
Warranty statement
Thank you for your purchase of QIAGEN instrumentation.
Your instrument has been carefully tested to ensure optimum
operating efficiency and reproducibility of results. QIAGEN
warrants that all new instrumentation manufactured by
QIAGEN will correspond to the product specifications and be
free from defects in workmanship and materials for a period
of twelve (12) months from the original date of shipment.
Repair or replacement of defective parts will be provided to
the purchaser during this time period provided the QIAGEN
instrumentation is operated under conditions of normal and
proper use, but not for damage caused by the customer. If
any part or subassembly proves to be defective, it will be
repaired or replaced at QIAGEN’s sole option, subsequent to
inspection at the factory, or in the field by an authorized
factory representative, provided that such defect manifested
under normal and proper use. The shipper will pay all
transport fees.
Limitation of warranties and remedies
THE FOREGOING WARRANTY IS QIAGEN’S SOLE AND EXCLUSIVE WARRANTY, AND
REPAIR OR REPLACEMENT OF DEFECTIVE PARTS IS THE SOLE AND EXCLUSIVE REMEDY.
THERE ARE NO OTHER WARRANTIES OR GUARANTEES, EXPRESS OR IMPLIED. THE
IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE
ARE EXPRESSLY EXCLUDED, TO THE FULLEST EXTENT PERMITTED BY LAW. (NOTE: SOME
STATES DO NOT PERMIT DISCLAIMERS OF IMPLIED WARRANTIES SO THIS LIMITATION
MAY NOT APPLY TO YOU). WITH THE EXCEPTION OF THE ABOVE-REFERENCED REPAIR
OR REPLACEMENT REMEDY, QIAGEN SHALL HAVE NO OBLIGATION OR LIABILITY OF
ANY NATURE WHATSOEVER WITH RESPECT TO THE QIAGEN INSTRUMENTATION,
WHETHER ARISING IN CONTRACT, TORT, STRICT LIABILITY, OR OTHERWISE, INCLUDING
BUT NOT LIMITED TO, LIABILITY FOR INDIRECT, CONSEQUENTIAL, INCIDENTAL
AND/OR SPECIAL, PUNITIVE, MULTIPLE AND/OR EXEMPLARY DAMAGES AND/OR OTHER
LOSSES (INCLUDING LOSS OF USE, LOST REVENUES, LOST PROFITS AND DAMAGE TO
REPUTATION), EVEN IF SUCH DAMAGES WERE FORESEEN OR FORSEEABLE, OR WERE
BROUGHT TO QIAGEN’S ATTENTION. IN NO EVENT SHALL QIAGEN’S LIABILITY TO
YOU EXCEED THE PURCHASE PRICE OF THE PRODUCT.
BioRobot EZ1 DSP Workstation User Manual 02/2007
B-1
Appendix B
Liability clause
QIAGEN shall be released from all obligations under its
warranty in the event repairs or modifications are made by
persons other than its own personnel, except in cases where
the Company has given its written consent to perform such
repairs or modifications.
All materials replaced under this warranty will be warranted
only for the duration of the original warranty period, and in
no case beyond the original expiration date of original
warranty unless authorized in writing by an officer of the
Company. Read-out devices, interfacing devices and
associated software will be warranted only for the period
offered by the original manufacturer of these products.
Representations and warranties made by any person,
including representatives of QIAGEN, which are inconsistent
or in conflict with the conditions in this warranty shall not be
binding upon the Company unless produced in writing and
approved by an officer of QIAGEN.
B-2
BioRobot EZ1 DSP Workstation User Manual 02/2007
Index
Index
C
Cartridge rack, 3-8
Connector panel, 3-5
Control panel, 3-1, 3-2
E
Elution tubes, 3-6
loading, 5-11
EZ1 Card, 3-1, 3-2
inserting, 5-2
removing, 5-3
slot, 3-2, 3-3
F
Filter-tips, 3-6
loading, 5-11
Fuse box, 3-5, 4-2
H
Heating positions, 3-8
Heating system, 3-1, 3-9
L
LCD, 3-2
M
Magnet, 3-9
Magnetic particles, 3-1
Maintenance, 6-1
daily, 6-4
decontamination, 6-6
weekly, 6-5
O
Operating conditions, 1-4
P
Piercing unit, 3-10
Pipettor head, 3-1, 3-9
Power cord, 3-5
Power requirements, 4-1
Power switch, 3-5
Protocol, 3-1
end, 5-7
starting, 5-6
stopping, 5-7
R
Racks, 3-5
Reagent cartridges, 3-1, 3-8
loading, 5-9
Reagent wells, 3-8
S
Safety
biological, 1-5
chemical, 1-6
electrical, 1-2
heat hazard, 1-7
mechanical hazards, 1-7
proper use, 1-2
symbols, 1-8
Sample tubes, 3-6
loading, 5-11
Servicing, 6-1
Status LEDs, 3-2, 3-4
Storage conditions, 1-4
Switching off, 5-5
Switching on, 5-4
Symbols, 1-8, 1-9
Syringe pumps, 3-9
T
Tip adapters, 3-9
Tip holders, 3-1, 3-6
BioRobot EZ1 DSP Workstation User Manual 02/2007
Index-1
Index
Tip rack, 3-6
Tips, 3-6
loading, 5-11
Tray, 3-9
removing, 5-9
Troubleshooting, 7-1
Index-2
W
Waste disposal, 1-6, A-4
Workstation door, 3-1, 3-2
closing, 5-6
opening, 5-6
Worktable, 3-1, 3-5
setting up, 5-8
BioRobot EZ1 DSP Workstation User Manual 02/2007
QIAGEN Distributors and Importers
Please see the back cover for contact information for your local QIAGEN office.
Argentina
Tecnolab S.A.
Tel:
(011) 4555 0010
Fax: (011) 4553 3331
E-mail: info@tecnolab.com.ar
Estonia
Quantum Eesti AS
Tel:
+372 7301321
Fax: +372 7304310
E-mail: quantum@quantum.ee
Malaysia
RESEARCH BIOLABS SDN. BHD.
Tel:
(603)-8070 3101
Fax: (603)-8070 5101
E-mail: biolabs@tm.net.my
Bosnia-Herzegovina
MEDILINE d.o.o.
Tel.: +386 1 830-80-40
Fax: +386 1 830-80-70
+386 1 830-80-63
E-mail: info@mediline.si
Greece
BioAnalytica S.A.
Tel:
(210)-640 03 18
Fax: (210)-646 27 48
E-mail: bioanalyt@hol.gr
Mexico
Quimica Valaner S.A. de C.V.
Tel:
(55) 55 25 57 25
Fax: (55) 55 25 56 25
E-mail: ventas@valaner.com
Hong Kong SAR
Gene Company Limited
Tel:
+852-2896-6283
Fax: +852-2515-9371
E-mail: info@genehk.com
New Zealand
Biolab Ltd
Tel:
(09) 980 6700
0800 933 966
Fax: (09) 980 6788
E-mail: biosciences@nzl.biolabgroup.com
Brazil
Uniscience do Brasil
Tel:
011 3622 2320
Fax: 011 3622 2323
E-mail: info@uniscience.com
Chile
Biosonda SA
Tel:
+562 209 6770
Fax: +562 274 5462
E-mail: ventas@biosonda.cl
China
Eastwin Scientific, Inc.
Order: +86-400-8182168
Tel:
+86-10-51663168
Fax: +86-10-82898283
E-mail: laborder@eastwin.com.cn
Gene Company Limited
Tel:
+86-21-64951899
Fax: +86-21-64955468
E-mail:
info_bj@genecompany.com (Beijing)
info_sh@genecompany.com (Shanghai)
info_cd@genecompany.com (Chengdu)
info_gz@genecompany.com (Guangzhou)
Genetimes Technology, Inc.
Order: 800-820-5565
Tel:
+86-21-54262677
Fax: +86-21-64398855
E-mail: order@genetimes.com.cn
Genetimes Technology International
Holding Ltd.
Tel:
+852-2385-2818
Fax: +852-2385-1308
E-mail: hongkong@genetimes.com.hk
Hungary
BioMarker Kft.
Tel:
+36 28 419 986
Fax: +36 28 422 319
E-mail: biomarker@biomarker.hu
India
Genetix
Tel:
+91-11-51427031
Fax: +91-11-25419631
E-mail: genetix@genetixbiotech.com
Oman
Al Mazouri Medical & Chemical
Supplies
Tel:
+971 4 266 1272
(ext. 301, 310, 311)
Fax: +971 4 269 0612
(ATTN: LAB DIVISION)
E-mail: shaji@almaz.net.ae
Pakistan
Pakistan Microbiological Associates
Tel:
+92-51-5567953
Fax: +92-51-5514134
E-mail: orderpma@comsats.net.pk
Peru
INMUNOCHEM S.A.C.
Indonesia
Tel:
+51 1 4409678
PT Research Biolabs
Fax: +51 1 4223701
Tel:
+62 21 5865357
E-mail: indonesia@researchbiolabs.com E-mail: inmunochem@terra.com.pe
Iran
Zist Baran (BIORAIN)
Tel:
+98 (21) 88066348 or
+98 (21) 88066349
Fax: +98 (21) 88214107
E-mail: info@biorain.com
Colombia
GENTECH – Genetics & Technology
Tel:
(+57)(4)2519037
Fax: (+57)(4)2516555
E-mail: gerencia@gentechcolombia.com
soporte@gentechcolombia.com
Israel
Eldan Electronic Instruments Co. Ltd.
Tel:
+972-3-937 1133
Fax: +972-3-937 1121
E-mail: bio@eldan.biz
Croatia
INEL Medicinska Tehnika d.o.o.
Tel:
(01) 2984-898
Fax: (01) 6520-966
E-mail: inel-medicinska-tehnika@zg.htnet.hr
Jordan
SAHOURY GROUP
Tel:
+962 6 4633290-111
Fax: +962 6 4633290-110
E-mail: info@sahoury.com
Cyprus
Scientronics Ltd
Tel:
+357 22 467880/90
Fax: +357 22 764614
E-mail: a.sarpetsas@biotronics.com.cy
Korea
LRS Laboratories, Inc.
Tel:
(02) 924-86 97
Fax: (02) 924-86 96
E-mail: webmaster@lrslab.co.kr
Czech Republic
BIO-CONSULT spol. s.r.o.
Tel/Fax: (+420) 2 417 29 792
E-mail: info@bioconsult.cz
Philekorea Technology, Inc.
Tel:
(02) 576-6540
Fax: (02) 576-6541
E-mail: support@philekorea.co.kr
Ecuador
INMUNOCHEM S.A.C.
Tel:
+51 1 4409678
Fax: +51 1 4223701
E-mail: inmunochem@terra.com.pe
Latvia
SIA “J.I.M.”
Tel:
7136393
Fax: 7136394
E-mail: jim@mednet.lv
Egypt
Clinilab
Tel:
52 57 212
Fax: 52 57 210
E-mail: Clinilab@link.net
Lithuania
INTERLUX
Tel:
+370-5-2786850
Fax: +370-5-2796728
E-mail: spirit@interlux.lt
Poland
Syngen Biotech Sp.z.o.o.
Tel:
(071) 798 58 50 - 52
Fax: (071) 798 58 53
E-mail: info@syngen.pl
Portugal
IZASA PORTUGAL, LDA
Tel:
(21) 424 7312
Fax: (21) 417 2674
E-mail: consultasbiotec@izasa.es
Qatar
Sedeer Medical
Tel:
+974 - 488 5218
Fax: +974 - 488 1988
E-mail: sedeer@qatar.net.qa
Romania
Zyrcon Medical S. R. L.
Tel:
+40 21 2245607
Fax: +40 21 2245608
E-mail:
virgil.dracea@zyrconmedical.ro
secretariat@zyrconmedical.ro
Saudi Arabia
Abdulla Fouad Holding Company
Tel:
(03) 8324400
Fax: (03) 8346174
E-mail:
sadiq.omar@abdulla-fouad.com
Singapore
Research Biolabs Pte Ltd
Tel:
6777 5366
Fax: 6778 5177
E-mail: sales@researchbiolabs.com
Slovak Republic
BIO-CONSULT Slovakia spol. s.r.o.
Tel/Fax: (02) 5022 1336
E-mail: bio-cons@cdicon.sk
Slovenia
MEDILINE d.o.o.
Tel:
(01) 830-80-40
Fax: (01) 830-80-70
(01) 830-80-63
E-mail: info@mediline.si
South Africa
Southern Cross Biotechnology
(Pty) Ltd
Tel:
(021) 671 5166
Fax: (021) 671 7734
E-mail: info@scb.co.za
Spain
IZASA, S.A.
Tel:
(93) 902.20.30.90
Fax: (93) 902.22.33.66
E-mail: consultasbiotec@izasa.es
Taiwan
TAIGEN Bioscience Corporation
Tel:
(02) 2880 2913
Fax: (02) 2880 2916
E-mail: order@taigen.com
Thailand
Theera Trading Co. Ltd.
Tel:
(02) 412-5672
Fax: (02) 412-3244
E-mail: theetrad@samart.co.th
Turkey
Medek Medikal Ürünler
ve Saglik Hizmetleri A. S.
Tel:
(216) 302 15 80
Fax: (216) 302 15 88
E-mail: makialp@med-ek.com
United Arab Emirates
Al Mazouri Medical & Chemical
Supplies
Tel:
+971 4 266 1272
(ext. 301, 310, 311)
Fax: +971 4 269 0612
(ATTN: LAB DIVISION)
E-mail: shaji@almaz.net.ae
Uruguay
Bionova Ltda
Tel:
+598 2 6130442
Fax : +598 2 6142592
E-mail: bionova@internet.com.uy
Venezuela
SAIXX Technologies c.a.
Tel:
+58212 3248518
+58212 7616143
+58212 3255838
Fax: +58212 7615945
E-mail: ventas@saixx.com
saixxventas@cantv.net
Vietnam
Viet Anh Instruments Co., Ltd.
Tel:
+84-4-5119452
Fax: +84-4-5119453
E-mail: VietanhHN@hn.vnn.vn
All other countries
QIAGEN GmbH, Germany
www.qiagen.com
Australia
Orders 03-9840-9800
Austria
Orders 0800/28-10-10
Belgium
Orders 0800-79612
Canada
Orders 800-572-9613
China
Fax 0800-79611
Finland
Orders 0800-914416
France
Orders 01-60-920-926
Germany
Ireland
Italy
Fax 800-713-5951
Fax 80-885944
Japan
Fax 02-33430426
Orders 800-18859
Sweden
Orders 020-790282
Switzerland
Orders 01293-422-911
USA
Orders 800-426-8157
1037181 124102347
Technical 02103-29-12400
Technical 1800-555-061
Technical 03-5547-0811
Technical 8002-2067
Fax 0800-0229593
Fax 800-18817
Technical 0800-0229602
Technical 800-18712
Fax 020-790582
Technical 020-798328
Fax 055-254-22-13
Fax 01293-422-922
Fax 800-718-2056
Offers 01-60-920-928
Technical 800-787980
Fax 8002-2073
Orders 055-254-22-11
UK
Technical 01-60-920-930
Fax 03-5547-0818
Orders 0800-0229592
Norway
Technical 0800-914413
Fax 1800-555-048
Orders 8002-2076
The Netherlands
Technical 80-885942
Fax 02103-29-22000
Telephone 03-5547-0811
Luxembourg
Technical 021-51345678
Fax 01-60-920-925
Orders 1800-555-049
Orders 02-33430411
Technical 800-DNA-PREP (800-362-7737)
Fax 0800-914415
Orders 02103-29-12000
Technical 0800/28-10-11
Technical 0800-79556
Fax 021-51342500
Orders 80-885945
Technical 1-800-243-066
Fax 0800/28-10-19
Orders 021-51345678
Denmark
Fax 03-9840-9888
Technical 055-254-22-12
Technical 01293-422-999
Technical 800-DNA-PREP (800-362-7737)
Sample & Assay Technologies