Leica EG1150 H
Paraffin Embedding
Station
Instruction Manual
Leica EG1150 H
V2.0 English - 10/2002
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
IMPORTANT NOTE
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and state-ofthe-art technology as we understand it following
thorough investigation in this field.
We are under no obligation to update the
present manual periodically and on an ongoing
basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we
exclude liability as far as permissible according
to the national legal system applicable in each
individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to
compliance with statements or other information in this manual.
Statements, drawings, illustrations and other information as regards contents or technical details
of the present manual are not to be considered as
warranted characteristics of our products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is it
possible to continuously improve the technology and manufacturing techniques used in our
products.
This document is protected under copyright
laws. Any copyrights of this document are retained by Leica Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Microsystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Microsystems Nussloch GmbH
Issued by:
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 (0)62 24 143-0
Fax:
+49 (0)62 24 143-200
Internet: http://www.histo-solutions.com
Leica EG1150H – Paraffin Embedding Station
3
Table of contents
1.
1.1
1.2
1.3
1.4
Important notes .......................................................................................................................................................... 5
Symbols used in the text and their
meanings ..................................................................................................................................................................... 5
Specified use and application ................................................................................................................................. 5
User group ................................................................................................................................................................... 5
Instrument type ........................................................................................................................................................... 5
2.
2.1
2.2
2.3
Safety .......................................................................................................................................................................... 6
Safety regulations ...................................................................................................................................................... 6
Safety instructions ..................................................................................................................................................... 6
Built-in safety devices ............................................................................................................................................... 8
3.
3.1
3.2
3.3
Instrument components and specifications ......................................................................................................... 9
Specifications ............................................................................................................................................................. 9
Overview – instrument parts .................................................................................................................................. 10
Instrument specifications ....................................................................................................................................... 11
4.
4.1
4.3
4.2
4.4
4.5
4.6
Commissioning ......................................................................................................................................................... 12
Unpacking and installation ..................................................................................................................................... 12
Package contents .................................................................................................................................................... 13
Location conditions.................................................................................................................................................. 13
Necessary assembly work ..................................................................................................................................... 14
Optional accessories ............................................................................................................................................... 16
Electrical connection .............................................................................................................................................. 17
5.
5.1
5.2
5.3
5.4
5.5
5.6
Operation ................................................................................................................................................................... 18
Instrument parts/functions ..................................................................................................................................... 18
Switching the instrument on .................................................................................................................................. 23
Control panel functions ........................................................................................................................................... 24
Operating modes ...................................................................................................................................................... 25
Time-program control ............................................................................................................................................. 26
Instrument heater ..................................................................................................................................................... 28
6.
6.1
6.2
Cleaning and maintenance .................................................................................................................................... 30
Cleaning the instrument .......................................................................................................................................... 30
Maintenance instructions ...................................................................................................................................... 31
7.
7.1
7.2
7.3
Troubleshooting ....................................................................................................................................................... 32
Possible faults ........................................................................................................................................................... 32
Replacing the halogen lamp ................................................................................................................................... 35
Changing a fuse ........................................................................................................................................................ 36
8.
Warranty and service ............................................................................................................................................. 38
4
Instruction Manual V 2.0 – 10/2002
1.
1.1 Symbols used in the text and their
meanings
Warnings and cautions
appear in a gray box and are marked
by a warning triangle .
Notes,
i.e. important information for the
user, appear in a gray box and are
marked with the symbol
.
Solvents and reagents that are inflammable are marked with this
symbol.
Important notes
1.2 Specified use and application
The Leica EG1150H is a modern paraffin embedding station with microprocessor control.
It is designed for embedding histological tissue
specimens in molten paraffin for use in pathology laboratories and only for the following tasks:
• Melt solid paraffin for sample embedding
and maintain the molten paraffin at the required temperature.
• Fill the molds, in which the tissue specimens
were placed, with paraffin.
• Heat and maintain the temperatures of embedding cassettes with specimens and
molds as well as the required forceps.
Any other use of the instrument will be
considered as improper use!
1.3 User group
(5)
ENTER
This warning symbol indicates the
surfaces on the instrument that are
hot during operation.
Avoid direct contact to prevent risk
of burning.
• The Leica EG1150H may only be operated by
qualified personnel.
• The user must read the operating instructions supplied and be familiar with all the instrument’s technical details before any work
on the instrument can be carried out.
Figures in brackets refer to item
numbers in figures.
1.4 Instrument type
Function keys that have to be
pressed on the input screen, are displayed in bold type and capital letters.
Leica EG1150H – Paraffin Embedding Station
All information in this instruction manual applies
only to the instrument type indicated on the title
page.
A name plate with
the serial number
is attached to the
back of the instrument.
5
2.
Safety
Please pay particular attention to the safety regulations and safety instructions in this
section.
Please also read this information, even if you are familiar with handling and using a
Leica instrument.
2.1 Safety regulations
This instruction manual contains important instructions and information regarding operational safety and maintenance of the instrument.
The instruction manual is an important part of
the product. It must be read carefully before using the instrument for the first time and always
kept near the instrument.
It may be necessary to add appropriate instructions to this instruction
manual, if required by existing national regulations on accident prevention or environmental protection
in the country of the operating authority.
This instrument was built and tested in accordance with the safety regulations for electrical
measuring, control, regulating and laboratory
devices as specified below:
• EN 61010-1/A2
• EN 61010-2-010
• EN 61326-1
• EN 61000-3-2
• EN 61000-3-3
• EN 61000-3-4.
In order to maintain this condition and ensure
safe operation, the operator must observe all
the instructions and warnings contained in this
instruction manual.
The protective devices on the instrument and its accessories must not be removed or
modified. The instrument may only be opened and repaired by authorized Leica servicing
technicians.
2.2 Safety instructions
The safety devices provided on this instrument by the manufacturer only constitute the basis
for protection against accidents. The main responsibility for accident prevention while using
the instrument rests with the employer where the instrument is operated, and with the persons
he appoints for operating, servicing or repairing the instrument.
To ensure a safe operation of the instrument at all times, the following instructions and warnings should be observed.
6
Instruction Manual V 2.0 – 10/2002
2.
Safety
Safety instructions – safety regulations on the instrument itself
Safety regulations marked with a warning triangle on the instrument itself mean that
when operating or exchanging respective parts of the instrument, the correct operating
steps as described in the instruction manual supplied, must be adhered to. Non-observance can cause accidents, injuries and/or damage to the instrument/accessories.
Certain surfaces of the instrument are hot during operation under normal
conditions. They are marked with this warning sign. Touching these surfaces
can cause burns.
Safety instructions – transport and installation
After unpacking the instrument it may only be transported in an upright position.
Place the instrument on a laboratory table and adjust it to a horizontal position.
The instrument must not be exposed to direct sunlight (window)!
Plug the instrument only into a grounded mains socket. The protective effect may not be
eliminated by an extension cable without a protective grounding conductor.
The instrument automatically recognizes the applied voltage/frequency.
The installation location must be well-ventilated; there should be no ignition sources
there of any kind.
The instrument may not be operated in hazardous locations.
Extreme temperature fluctuations between storage facility and setup site as well as high
humidity may cause condensation to form. In this case, wait at least two hours before
switching on.
Safety instructions – working with the instrument
Paraffin is flammable and should therefore be handled with due care. Do not use sharp
tools to remove solidified paraffin from the work areas, as this may destroy the coating on
the surface. Use the plastic spatula supplied with the instrument.
During operation, the paraffin reservoir, mold warmer, cassette warmer, work area as
well as the forceps holder are hot.
Risk of burning!
Do not store any combustible and flammable substances near the instrument. There is a
fire hazard if work with an exposed flame (e.g. Bunsen burner) is carried out in the direct
vicinity of the instrument (solvent vapors). Therefore a minimum safety distance of 2
meters must be adhered to!
Leica EG1150 H – Paraffin Embedding Station
7
2.
Safety
Hazards – servicing and cleaning
Switch off the instrument each time before servicing and pull out the mains plug.
When using cleaners, please comply with the safety instructions of the manufacturer and
the laboratory safety regulations.
Before changing defective fuses, the instrument has to be disconnected from the mains.
Only fuses that are easily accessible may be replaced by the user.
Set the standby switch to “Standby“ ( ) when replacing the halogen lamp and pull the
power cable out of the mains socket.
During operation and cleaning, do not allow any liquid to penetrate inside the instrument
and the transporting arm.
2.3 Built-in safety devices
The instrument is equipped with the following safety features and devices:
Fuses in the heating elements
All resistance mats of the instrument are equipped with overheating fuses,
which switch the heating element off if overheated.
Automatic circuit breaker in standby switch
An automatic circuit breaker is located in the standby switch. This circuit
breaker separates the power electronics from the mains power supply in
the event of a short-circuit.
In this case, the standby switch jumps to the position “0“ = Off.
Note that the only way the user has for complete disconnection from the mains supply is
disconnection of the mains plug.
8
Instruction Manual V 2.0 – 10/2002
3. Instrument components and specifications
3.1 Specifications
General data
Approvals:
Power voltages:
Input:
Class of protection1:
Degree of pollution1:
Overvoltage category:
Operating temperature range:
Working temperatures:
Relative air humidity:
1)
Fuses
The approval symbols relating to this instrument are located
on the rear of the instrument next to the nameplate.
100-120 V, 50/ 60 Hz
230-240 V, 50/ 60 Hz
1100 VA
I
2
II
+18 °C to + 40°C
55 °C to 70 °C, adjustable in 5 degree (K) increments.
maximum 60 %, non-condensing.
according to IEC-1010, UL 3101, EN 61010
Standby switch:
Fine-wire fuses 6.3 x 32 mm:
Fuse type:
Circuit breaker manufactured by ETA,
model 3120-F421-P7T1-W01D-5A
T 2 A; T 4 A; 2 x T 3.2 A; T 6.3 A
Co. Schurter: type Fst
Dimensions and weight
Dimensions:
Height:
Width:
Depth:
Weight:
360 mm
500 mm
640 mm
approx. 22 kg
Capacities
Paraffin reservoir:
Cassette warmer:
Mold warmer:
approx. 3 l
approx. 100 cassettes
approx. 50 molds
Programmable parameters
Temperature:
Time:
Leica EG1150 H – Paraffin Embedding Station
Paraffin reservoir/dispenser
(heating-up time = 4h)
Mold warmer, cassette warmer, work area
Working days, current weekday
Working times (start, end), time
9
3.
Instrument components and specifications
3.2 Overview – instrument parts
Standby
switch
Paraffin reservoir
with lid
Control panel
Work area illumination
Dispenser
Cassette warmer
lid
Cassette warmer
Forceps holder
Work area
Mold warmer
lid
Mold warmer
Paraffin collection tray
left
Paraffin collection
tray right
Refrigeration spot
Fig. 1
10
Instruction Manual V 2.0 – 10/2002
3. Instrument components and specifications
Instrument rear view
Secondary fuses F1-F3
Secondary fuses
F4-F5
Heat sink
Port for power cord
Port for
foot switch
Sliding elements
3.3 Instrument specifications
Fig. 2
• Paraffin reservoir with a capacity of 3 liters.
• The paraffin flow is activated by means of a height-adjustable,
pivotable clip – activated either manually by the mold or a foot switch
(optional).
• Controllable flow rate.
• Removable, heatable (indirectly, via the work surface) paraffin collection trays.
• Spacious, easy-to-clean, heated work area, with integrated refrigeration spot, also for extra large cassettes (‘Super Mega Cassettes’) with
paraffin flow system.
• Warmers for cassettes and/or molds with sliding lid, removable and interchangeable.
• Removable, heated forceps holder for 6 forceps, accessible from both
sides.
• Optimum illumination of the work surface by individually adjustable
halogen lamp.
• Temperature range of cassette and mold warmer, work area and paraffin reservoir adjustable from 55°C to 70°C.
• Permanent temperature display for all the work areas.
• The beginning and end of the work time and work days can be programmed.
Leica EG1150 H – Paraffin Embedding Station
11
4.
Commissioning
4.1 Unpacking and installation
The unpacking instructions are attached to the outside of the transport crate. Fig. 3
shows the design of the original packaging.
The numbers indicate the sequence of disassembling and reassembling.
• To lift up the instrument (6), hold it at the
front and rear of the base plate (Fig. 4) and
lift it from the foam padding (8).
Important!
Lifting the instrument by the dispenser (10) or paraffin reservoir (11)
can cause serious damage.
• After unpacking the instrument it should only
be transported in a horizontal position.
• Two slide faces (9) at the rear of the base
plate help to reposition the instrument on the
table. To reposition, slightly lift the instrument at the front of the base plate and slide
it on the slide faces.
• The instrument should be set up in such a
way that the air circulation is not affected. A
smooth operation is ensured only when the
instrument is set at a minimum distance of
15 cm from walls and furniture.
11
10
9
Fig. 3
12
Fig. 4
Instruction Manual V 2.0 – 10/2002
4. Commissioning
4.2 Location conditions
• Stable, vibration-free laboratory table with horizontal, flat table top, as
far as possible vibration-free ground.
• The instrument must not be placed in the vicinity of the air outlet of an
air conditioner and must be shielded from strong sunlight (window).
• To ensure a fully functional heat sink, there must be gap of at least
15 cm behind the instrument.
• The vicinity of the work area must be free of oil and chemical vapors.
The installation location must be well-ventilated; there should
be no ignition sources there of any kind. The instrument should
not be operated in hazardous locations.
4.3 Package contents
The basic equipment for the Leica EG1150 H contains the following components:
1 Leica EG1150 H basic instrument
1 set of power cords for heater equipment:
1 power cord “EU”
1 power cord “UK” ST/BU F-5A
1 power cord “USA-C-J”
2 cassette/mold warmers, removable
2 lids for cassette/mold warmers
1 lid for paraffin reservoir
1 paraffin spatula
1 forceps holder, removable
1 filter screen with holder *
2 paraffin collection trays
1 halogen lamp (12 V, 5 W) *
1 replacement halogen lamp 5 W.
* The parts marked * are already on the instrument.
Leica EG1150H – Paraffin Embedding Station
1 set of spare fuses:
1 fuse T 2.0 A
2 fuses T 3.2 A
1 fuse T 4.0 A
1 fuse T 6.3 A
1 instruction manual
Optional accessories:
1 magnifier (2-fold)
1 foot switch for controlling the paraffin exit.
Please compare the delivered components
against the packing list, delivery note, and your
order.
Should there be any discrepancy, please contact the Leica distributor handling your order.
13
4.
Commissioning
4.4 Necessary assembly work
Install the following accessories and make the appropriate adjustments to
make the instrument ready for use:
• Install accessories.
• Adjust instrument feet if necessary.
• Install magnifier (optional).
• Connect foot switch (optional).
• Establish the electrical connections.
Install accessories
 Install mold warmer (2) and cassette warmer (3).
Depending on the preferred working direction, the two heated trays (11)
can be used for molds or cassettes as required.
‚ Close mold/cassette warmer with the corresponding lids (4), (5).
ƒ Push the paraffin collection tray (6) into the respective guide
underneath the work top.
„ Install the forceps holder (1).
… Insert the filtering screen (7) in the paraffin outlet hole (8) inside the
paraffin reservoir so that the black O-ring seals the hole.
† Place the lid (9) on the paraffin reservoir.
Adjustable instrument feet
The four feet are height-adjustable to allow the instrument to be adjusted to
match the height of other devices or to compensate slight depressions on
the surface.
Lift the instrument slightly and turn the instrument foot (10 in Fig. 5) to adjust
it to the proper height.
To ensure a safe stand, all of the four feet must be adjusted to
the same height.
14
Instruction Manual V 2.0 – 10/2002
4.
Commissioning
Installing the accessories
…
9
8
7
†
4
5
‚
‚


2
11
11
„
10
ƒ
1
3
6
ƒ
6
Fig. 5
Leica EG1150 H – Paraffin Embedding Station
15
4.
Commissioning
4.5 Optional accessories
Magnifying glass
The magnifying glass provides a magnified view of the work area. When
properly adjusted, an enlarged view of the dispenser and refrigeration
spot is available.
5
1
1
2
3
4
5
4
3
Fig. 6
Installing the magnifying glass
• On the dispenser (5) a tap hole (1) is provided, which is closed with a
nylon screw (2).
• Remove the screw (2) with a screwdriver and store it in a safe place.
Screw the knurled screw (3) of the stand (4) into the tap hole and align
the magnifier.
Foot switch
The foot switch can be used to actuate the dispenser
valve, leaving the operator’s hands free.
• To connect and use the foot switch, see Chapters 4.6
and 5.1.
Fig. 7
16
Instruction Manual V 2.0 – 10/2002
4. Commissioning
4.6 Electrical connection
The instrument MUST be connected to a grounded mains socket.
The instrument is supplied with a set of different power cords. Only the power cord intended for the local power supply (socket) may be used.
Do not use an extension cord.
1
4
6
2
3
5
Fig. 8
Before connecting the power cord to the foot switch, ensure that the standby switch (1)
(control panel, front) is set to ‘O’ (‘O’ = OFF).
Connecting the power cord
• Connect the plug (2) of the power cord to the
connecting port (3).
• Plug the power cord into the wall outlet.
Leica EG1150H – Paraffin Embedding Station
Connecting the foot switch (optional)
• Insert the plug (4) of the foot switch into the
connecting port (5) on the rear of the instrument.
• Tighten the screws (6) of the plug.
17
5.
Operation
5.1 Instrument parts/functions
1
2
11
Recycled paraffin may not be used
in the Leica EG1150H due to the danger of contamination.
1
14
13
13
2
12
Fig. 9
The flow cannot be stopped completely with the metering screw
(11). It must not be turned whilst
cold!
18
Paraffin reservoir (1)
The paraffin reservoir has a capacity of 3 liters.
The paraffin temperature is adjustable between
55 °C and 70 °C in 5 degree (K) increments. The
lid should always be in place, otherwise the adjusted temperature cannot be maintained.
An overtemperature cutout is provided to prevent overheating the paraffin if temperature
control fails.
A built-in filtering screen prevents any particles
contained in the paraffin from getting in the paraffin block.
Dispenser (2)
The dispenser is heated separately. The temperature adjustment of the dispenser and paraffin reservoir is coupled.
The quantity of paraffin released from the filler
tube (14) can be adjusted continuously with the
metering screw (11).
The dispenser handle (12) is used for manually operating the paraffin flow. It is provided with an extension clip (13). The extension clip is height-adjustable and can be shifted to the side (left or
right) depending whether the dispenser handle is
released with the mold or the finger .
The dispenser handle can be operated by simply pushing the mold (or the finger) against the
extension clip. Slightly pushing the handle
backwards will open the dispenser valve ‚.
Once released, the handle flips back in the original position and the valve shuts.
Instruction Manual V 2.0 – 10/2002
5. Operation
Work area (3)
The temperature of the work area can be adjusted between 55 °C and 70 °C in 5 degree
(K) increments.
This includes the embedding area, forceps
holder (5) and the refrigeration spot (4).
A groove around the work area and several
drain holes (15) are provided to allow liquid
paraffin to drain rapidly.
5
3
4
3
Refrigeration spot (4)
The refrigeration spot is an integral part of the
work area. Its position directly in front of the
embedding area enables convenient working
in ergonomically most favorable conditions.
To orientate the samples, the mold is filled
about one third with liquid paraffin. The liquid
paraffin begins to solidify rapidly on the refrigeration spot.
While the paraffin is semi-liquid, the sample
can be oriented as required. Finally, the mold
can rapidly be filled up with paraffin.
15
4
While orienting the tissue, the
paraffin should not become too
solid, as this may cause different
phases in the finished block, including fissures inside, as a result
of which the block may break during sectioning.
5
Fig. 10
The forceps holder is separately
heated to approximately 70 °C.
Risk of burning!
Leica EG1150H – Paraffin Embedding Station
Forceps holder (5)
The removable forceps holder under the dispenser can accommodate up to 6 forceps.
19
5. Operation
Paraffin collection tray (8)
Two indirectly-heated paraffin collection
trays for excess paraffin are located under
the work surface.
The paraffin collection tray must
be emptied daily. To prevent contamination, do not reuse paraffin
collected in this tray.
If the instrument is operated without the paraffin collection trays
there is risk of burning.
7
6
8
8
Mold warmer and cassette warmer (16)
Depending on the preferred working direction,
the two heated trays can be used for molds or
cassettes (6,7). The temperature is adjustable
between 55 °C and 70 °C.
A removable receptacle (16) can be placed in
each of the trays.
Each receptacle can hold approx. 100 cassettes
or approx. 50 molds.
A lid (17) is provided for each of the trays (16) to
prevent loss of heat and contamination inside
the tray. For easy access, the lid can be opened
and folded back.
17
16
Fig. 11
When working with half-open lids (17),
raise the temperature one level to ensure that the paraffin remains molten.
20
Only operate the cassette and
mold warmer and paraffin reservoir with lid; otherwise the set
temperature cannot be maintained.
Instruction Manual V 2.0 – 10/2002
5. Operation
Standby switch (8)
After the unit has been commissioned, the
standby switch should only be used if the unit
is to be switched off for a longer period of
time.
21
8
9
20
In daily routine operation, the ON/OFF button
on the control panel should be used.
Pressing the ON/OFF button (20) switches the
instrument into active standby mode.
All displays are switched off, only the LED
(21) of the ON/OFF button remains lit.
If programmed procedures are to
be carried out: the standby
switch (8) must be switched on
and the unit must be in standby
mode.
For more information, see
Chapter 5.4.
18
19
Fig. 12
Leica EG1150H – Paraffin Embedding Station
Work area illumination (9)
A halogen lamp provides homogeneous diffuse illumination of the embedding area and
refrigeration spot. This gives an optimum
view of the embedding process and tissue
orientation.
The lamp should be adjusted in a way that it
is switched on and off only with the lamp button on the control panel. For this purpose,
turn the cap (18) in the position where the
two “I“ “I“ marks (19) are located one above
the other.
To access the halogen bulb, the cap (18) can
be removed.
(For replacement of the halogen lamp see
Chapter 7.2)
21
5.
Operation
Foot switch (22) optional
The foot switch (22) actuates the magnet
valve of the dispenser.
22
11
It must be connected as described in
Chapter 4.6.
The plug of the foot switch
MUST be screwed onto the
socket. Otherwise hot paraffin may escape even when
the foot switched is not being pressed.
14
12
Pressing the foot switch opens the
valve, releasing it closes it. This keeps
the operator’s hands free to work with
the instrument.
The flow volume can be adjusted with
the metering screw (11).
The dispenser handle (12) is not required
when using the foot switch and can be
folded upwards.
Proceed as follows:
• Set the metering screw (11) to minimum.
• Carefully fold the dispenser handle
(12) back/up with your thumb and index finger.
12
Take care when folding
back the dispenser handle!
Hot paraffin may come out of
the filler tube (14).
DANGER OF BURNS!
14
Fig. 13
22
Instruction Manual V 2.0 – 10/2002
5. Operation
5.2 Switching the instrument on
Fig. 14
• Activate the standby switch (left-hand side, next to control panel) (see
Figs. 14, 16).
• All LEDs of the operating panel light up briefly and the installed software version is shown on the display for around 2 seconds.
All displays go out, the unit changes to standby mode.
The green LED in the ON/OFF button indicates that the instrument is
ready to operate.
• Press the ON/OFF button down for approx. 2 seconds to
switch over to the operating mode. The time indicator
flashes on the display, indicating that the instrument has
been disconnected from the mains supply.
Press any key to acknowledge.
The unit's normal operating mode is STANDBY/ON; i.e. switch
on/off with the ON/OFF key only.
Use the standby switch only if the device is to be switched off
for an extended period.
Fig. 15
• The heaters become active and the LEDs indicate the values last set.
The LED’s which indicate the set temperatures of the heated areas (1, 3
and 4, fig. 17) will flash from time to time over the entire length of the
heating phase (compare “Indication of the heating intervals”).
The LED of the paraffin reservoir (2, fig. 17) flashes in time intervals of
1 second each while the intensified heating of the paraffin reservoir is
active (4 hours).
If temperature settings are modified during the heating phase, the instrument will store the new settings and the heating phase will then
correspondingly take longer.
• Fill the paraffin reservoir with paraffin.
Prior to leaving the factory, the Leica EG1150H is tested thoroughly under laboratory conditions. For this reason, you will find
a small quantity of clean, hardened ”Leica Histowax” in the instrument. You can work with this paraffin without any problem.
Leica EG1150 H – Paraffin Embedding Station
23
5.
Operation
5.3 Control panel functions
The control panel beside the standby switch (8) consists of a foil keyboard
with push buttons, LEDs and a single-line display.
It is divided into two control areas:
temperature setting and display and instrument control and programming.
8
Temperature control
and display
Set time
LED display for the
individual heated areas
6
5
1
2
3
24
Display:
standard indication
is real time
Fig. 16
Illumination
On/Off
7
4
Temperature setting buttons
of the heated areas
1234-
Instrument control and
programming
Cassette/mold warmer, left
Paraffin reservoir and dispenser
Work area
Cassette/mold warmer, right
Working day
selection
Set buttons for
Programming
Activation of
enhanced mode
LEDs for mode indication of:
5 - Working day, weekday
6 - Start/End times, real time
7 - Current working day
Selector switch for
standby mode
or
working day ON/OFF
Fig. 17
Instruction Manual V 2.0 – 10/2002
5.
Operation
5.4 Operating modes
21
Standby mode
The display and all LEDs are switched off in standby mode. The unit
switches on or off automatically at the programmed start and ending
times.
Only the green LED (21) in the ON/OFF button indicates that the instrument
is ready to operate.
Enabling operating mode
• Hold down the ON/OFF button for approx. 2 seconds.
• The instrument switches from standby mode to operating mode. The
LEDs of the currently selected values are illuminated, the display reads
the current time.
The time in the display flashes when switching to operating
mode if the device was disconnected from AC power for an extended period (> 5 min).
Acknowledge the flashing display by pressing any button and
check whether all required heating phases (especially the paraffin reservoir) are complete.
24-hour mode – working in shift mode
If the switch-on and switch off times of the timer are set to the same
value, the instrument will run continuously, even on days that are not defined as work days.
Example: switch-on time = 00:00 and switch-off time = 00:00.
To set the timer, see Chapter 5.5.
Important
The timer is disabled if the switch-off time is before the
switch-on time.
E.g. switch-on time: 08:00 a.m. and switch-off time: 06:00 a.m.
In 24-hour mode, activate enhanced mode (see Chap. 5.6) to accelerate the melting process after replenishing solid paraffin.
Leica EG1150 H – Paraffin Embedding Station
25
5.
Operation
5.5 Time-program control
The value shown in the display must be set to the current local time to ensure the correct operation of the time-program control.
Setting the time
• Press the CLOCK button as many times as required until the green
“TIME“ LED lights up.
• Set the current time with the
/
buttons.
The required value is reached faster when holding down the button in
question.
Start time
The start time is the time at which the instrument automatically switches
from standby to operating mode.
Setting the start-of-work and end-of-work times:
• Press the CLOCK button as many times as required until the green LED
“START“ lights up.
• Set the start time with the
/
buttons.
The required value is reached faster when holding down the button in
question.
End time
Automatic switch-over of the unit from operating to standby mode takes
place at the set ending time.
Setting the start-of-work and end-of-work times:
Fig. 18 • Press the CLOCK button as many times as required until the green
“END“ LED lights up. Set the time as described above.
All set times are stored – even if the unit is switched off with
the standby switch – until they are changed.
26
Instruction Manual V 2.0 – 10/2002
5. Operation
Weekdays/working days
The automatic switch-on feature is linked with the individual weekdays. It
is therefore necessary to define the days for which the automatic switchon feature shall operate.
Only on those weekdays that have been defined as working
days, the instrument will be at the required temperature and
ready to operate.
Setting the current weekday:
• Press the DAY button as many times as required until the green “TODAY“
LED lights up. The green LED of the current weekday is illuminated.
• If necessary, se the current day with the
ated LED lights up.
/
buttons – the associ-
Defining the working days:
• Press the DAY button as many times as required until the green LED
“WORKING DAY“ lights up. The LEDs of the weekdays defined as
working days light up, while the LED of the current day flashes.
• Use the
/
buttons to select the day to be defined or canceled as
working day.
The LED of the selected day (FRI) flashes.
• Press the ON/OFF button.
If that day had not been a working day (LED off), it will now be added to
the working day list (LED on).
Otherwise (see Fig. 19, FRI was defined as a working day, LED on), the
day will no longer be defined as a working day (LED off).
Fig. 19
If the programmed values are to be effective on the following
days (the instrument is operational at the programmed time and
switches off automatically when the switch-off time is
reached), the standby mode must be active --> press ON/OFF.
Leica EG1150 H – Paraffin Embedding Station
27
5.
Operation
5.6 Instrument heater
Setting temperature values
The temperatures for the four heated areas can
be adjusted separately in a range from 55 °C to
70 °C in 5 degree (K) increments.
1
2
3
When setting the temperature,
please observe the paraffin
manufacturer’s specifications for
the maximum permissible temperature.
4
Fig. 20
1 - Cassette/mold warmer, left
2 - Paraffin reservoir/dispenser
3 - Temperature of work area
4 - Cassette/mold warmer, right
Press the buttons for the temperature range
once to increase the value by 5 K. The red LED
for the associated temperature value will light
up – after 70 °C has been reached, the temperature will revert to 55 °C.
Once set, the temperature value for one range
will be retained until it is changed.
Indication of the heating intervals
In the temperature range indication field, the LED for the temperature selected at a time will always light. While heating for the area in question is
active, the LED flashes as long as heating continues.
It is possible to deactivate the indication (= flashing) of the heating activity.
Proceed as follows:
• Switch off the instrument with the standby switch (
, not ON/OFF).
+
• Hold down the WORK AREA button and switch the instrument on again
with the standby switch.
+
• To return to the “flashing” mode, switch off the instrument with
standby switch as described under 1.
Hold down the PARAFFIN DISPENSER button when switching back on.
Fig. 21
28
Instruction Manual V 2.0 – 10/2002
5.
Operation
Advance times
Display
To ensure that the instrument is ready (all working temperatures reached)
at the programmed start time, the various sections are activated in advance as follows:
Paraffin reservoir:
heating starts 4 hours before work starts.
Work area:
heating starts 4 hours before work starts.
Dispenser tube:
heating starts 1 hour before work starts.
Refrigeration spot: refrigeration begins 1 hour before work starts.
During the heating phase, the display reads – – : – – ; the colon flashes in
intervals of seconds.
The advance times cannot be modified.
Enhanced mode
A large amount of heat is needed for melting paraffin. This is only accounted for in standby mode for a respective preliminary time. In the operating mode, the paraffin reservoir is heated just enough to maintain the
paraffin at the selected temperature. Therefore the melting process can
be sped up by increasing the heat supply (enhanced mode) whenever
necessary (e.g. when solid paraffin has to be added to the reservoir when
working in shifts). The paraffin reservoir is then heated for a period of four
hours to a higher temperature (enhanced mode).
To activate the enhanced mode, push and hold
and
buttons simultaneously until all
the
four set value LED’s of the paraffin reservoir
light up for an instant indicating thus that the
enhanced mode has been activated.
The selected set value LED flashes while enhanced mode is enabled.
The enhanced mode can be switched off at any
time by pressing and holding and again until all
four LED’s of the paraffin reservoir light up
briefly indicating that the enhanced mode has
been switched off.
press simultaneously
Fig. 22
Leica EG1150H – Paraffin Embedding Station
29
6.
Cleaning and maintenance
6.1 Cleaning the instrument
Do not use xylene for cleaning. Xylene vapors are heavier than air and can ignite at a
considerable distance from the source of heat.
Fire hazard!
To avoid scratching the surface of the instrument only the plastic spatula that is supplied
with it should be used for cleaning – on no account metal tools!
Work area
• All common laboratory cleaning products suitable for the removal of paraffin
(e.g. Paraguard or xylene substitutes) can be used to clean the work area.
• Avoid prolonged contact of organic solvents on the surface of the instrument.
Paraffin reservoir
• Keep contaminants out of the paraffin reservoir.
• Ensure that a residual amount of paraffin remains in the reservoir after draining to prevent solid contaminants from entering the dispenser.
• Absorb this paraffin with tissue or a paper towel. Do not remove the screen until the residual paraffin has been removed.
• The interior surfaces of the reservoir can then be cleaned with a tissue.
Forceps holder
• The forceps holder is often a source of contamination and extremely susceptible to dirt.
Therefore, clean the forceps holder thoroughly.
Important!
The forceps holder is separately heated to approximately 70 °C. Risk of burning!
30
Instruction Manual V 2.0 – 10/2002
6. Cleaning and maintenance
Paraffin collection tray
• Before the paraffin collection tray drawers can be emptied, any excess paraffin on the
work area must be removed with cellulose wadding in order to prevent any paraffin
from penetrating into the instrument.
Use care with paraffins with a low melting point - risk of burning when removing the paraffin collection trays due to liquid paraffin.
• Only remove and empty the paraffin collection trays while they are warm.
• The paraffin in the collection trays must not be reused. Danger of paraffin carry-over
into the instrument.
• Empty both paraffin collection trays regularly in order to prevent them from overflowing
into the instrument. While emptying intervals may vary depending on use, the trays
should be emptied at least daily.
If the paraffin collection trays are not emptied regularly, excess paraffin may flow into the
instrument or onto the work surface.
In addition to the risk of burns, this may damage the instrument.
6.2 Maintenance instructions
Only Leica service technicians are authorized to open the instrument for maintenance and
repair work.
The Leica EG1150H is virtually maintenance-free.
Please observe the following points to ensure the instrument’s reliable function over extended periods:
• Clean the instrument daily with care.
• Regularly remove dust from the ventilation slots on the back of the instrument with a
brush or vacuum cleaner.
• Have the instrument inspected at least once a year by an authorized Leica customer
service technician.
• Enter into a service contract at the end of the warranty period. For more information,
contact the relevant technical service center.
Leica EG1150 H – Paraffin Embedding Station
31
7.
Troubleshooting
7.1 Possible faults
This chapter will help to correct problems that may occur when working
with the Leica EG1150H.
If a problem cannot be remedied by following the instructions in this chapter, please contact your Leica technical service center.
For further instructions, please refer to Chapter 8.
The table below includes the most common problems that may occur
when working with the instrument, including possible causes and corrective action.
Problem
Possible cause
Corrective action
1. Display
On the display the
indication
is shown.
- The instrument is in the
heating phase (standby
mode)
- This is not an error
message!
The instrument will switch
to the program mode at the
preselected time.
- Standby switch not turned
on
- Standby switch ON.
The colon flashes.
2. Instrument does not
switch on
or
32
- cut-out function of the
standby switch activated.
- Check unit connection, then
turn standby switch ON.
- ON/OFF button not pressed
long enough.
- Hold down ON/OFF button
for several seconds
(at least 2 secs).
Instruction Manual V 2.0 – 10/2002
7. Troubleshooting
Problem
Possible cause
Corrective action
3. Paraffin reservoir
Paraffin does not melt or
melts only very slowly.
- Selected temperature too
low.
- Increase temperature for
paraffin reservoir.
- No enhanced mode set.
- Activate enhanced mode.
(see Chapter 5.6)
- Temperature indication
incorrect, or no heating of
paraffin reservoir.
- Check temperature settings
and repeat if necessary.
- Start-of-work time selection incorrect.
- Check start-of-work time.
- Fuse for the heater of the
paraffin reservoir activated.
- Check fuses and replace as
required
(see Chapter 7.3)
- Instrument malfunction
- Contact customer service.
- Paraffin not completely
molten.
- Wait until paraffin is entirely molten; then check
again.
Paraffin drops when valve
is shut.
- The paraffin outlet is
clogged or the magnetic
switch defective.
- Contact customer service.
Non-homogeneous paraffin
flow (e.g. air pockets).
- Air pockets in dispenser at
the start.
- Keep dispenser open at
maximum flow for a period
of time.
4. Paraffin dispenser
No paraffin flow.
Leica EG1150 H – Paraffin Embedding Station
33
7.
Troubleshooting
Problem
5. Illumination
does not work.
6. Individual heated areas
(despite correct temperature indication) heating
does not work.
7. Error message in display
On the display
the indication
is shown.
Program and time are lost.
Possible cause
Corrective action
- Cap of the work area
halogen lamp incorrectly
positioned.
- Check adjustment of
lamp cap.
(see Figures 12 / 23)
- Bulb defective.
- Replace bulb
(see Chapter 7.2)
- Fuse defective.
- Replace fuse
(see Chapter 7.3)
- Fuse for this heated area
has responded.
- Replace fuse.
(see Chapter 7.3)
- Heater defective.
- Contact customer service.
- The battery is empty
and also
- Acknowledge error with
any key and continue
working after reprogramming.
- Power failure or
- Device switched off with
standby switch or
Ask technical service to
replace battery.
- Mains plug pulled.
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Instruction Manual V 2.0 – 10/2002
7. Troubleshooting
7.2 Replacing the halogen lamp
Only use the original replacement lamp delivered with the maintenance kit.
Before you replace the lamp, switch off the unit at the standby
switch and disconnect the mains plug.
To replace the halogen lamp, please proceed as shown in Fig. 23:
1
2
5
1
2
3
4
1. Turn the upper, moveable cap on the
lamp (1) until the markings are in
alignment as shown in the figure. 
2. Remove cap – this can be done only in
the position shown in ‚.
3. Pull out defective lamp (2). ƒ
If this cannot be done by hand, do not
use any hard or acute tools (e.g.
screwdriver).
Wait until the lamp has cooled down.
Turn lamp housing upside down and
lightly knock on the housing until the
lamp falls out. „
4. Hold the new lamp with care (do not
touch the glass bulb!) and insert in the
housing. …
5. Replace cap (observing correct alignment!) and turn to the “I“ to “I“ position. †
6
Fig. 23
Leica EG1150 H – Paraffin Embedding Station
35
7.
Troubleshooting
7.3 Changing a fuse
Before changing a fuse switch the instrument off and pull out the mains plug.
Use ONLY the provided replacement fuses.
Important!
The instrument has a number of circuits that are protected by fuses with different values.
Observe the following instructions exactly to ensure that the correct spare fuse is used
for the matching fuse holder.
The instrument has five electrical areas with separate secondary fuse
protection. The secondary fuses are located in individual fuse inserts at
the back of the instrument.
The secondary fuses are secured with two metal plates (6,7) that must be
removed first.
1
2
4
5
3
6
7
Fig. 24
The individual fuses apply to the following areas:
1 - Peltier element of the refrigeration spot and work area illumination.
2 - Heating for forceps holder and paraffin dispenser.
3 - Electronics.
4 - Heating for work area and cassette warmer right.
5 - Heating for paraffin reservoir and cassette warmer left.
36
Instruction Manual V 2.0 – 10/2002
7. Troubleshooting
Select the correct spare fuse
This marking (2A) on the fuse and the labeling next to the fuse holder on the rear
of the instrument MUST agree!
Fig. 25
The metal caps on the spare fuses are labeled –
the labeling looks similar to that shown in the
figure above, but can vary depending on the
type of fuse.
The label information which is important for the
correct use of the fuse is its maximum current
(in the marked example: T2A).
Change fuse
Using a screwdriver (8) lightly press in the fuse
holder (9) and then turn anti-clockwise one 1/4
turn and let go.
The fuse holder is pressed out and can be removed.
Take the defective fuse (10)
out of the fuse holder (9) and
replace it by the selected
spare fuse.
Insert the fuse holder with
the spare fuse, press in using screwdriver (1) and secure by turning it clockwise
1/4 turn.
Replace fuse cover (6,7).
10
8
9
On the back there are five different fuse holders (Fig. 24).
One spare fuse must be selected from the five spare
fuses available that has the
marking 2A written on it.
(see Fig. 25, left)
Only the spare fuse with the
marking 2A may be inserted
in this fuse holder (T2A).
This is also applicable in the
same way for the other fuse
holders.
9
Fig. 26
Leica EG1150H – Paraffin Embedding Station
37
8.
Warranty and service
Warranty
Leica Microsystems Nussloch GmbH guarantees that the contractual
product delivered has been subjected to a comprehensive quality control
procedure based on the Leica in-house testing standards, and that the
product is faultless and complies with all technical specifications and/or
agreed characteristics warranted.
The scope of the warranty is based on the content of the concluded
agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall
apply exclusively.
Technical service information
If you require technical service or replacement parts, please contact your
Leica sales representative or dealer who sold the product.
Please provide the following information:
•
•
•
•
Model name and serial number of the instrument.
Location of the instrument and name of the person to contact.
Reason for the service call.
Date of delivery.
Decommissioning and disposal
The instrument or parts of the instrument must be disposed of in compliance with the local laws.
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Instruction Manual V 2.0 – 10/2002