Auto Page MA-200 Instruction manual

M498E
08.10.NF.1 (2/4)
Inverted Metallurgical Microscope
ECLIPSE MA200
Instructions
Introduction
Thank you for purchasing a Nikon product.
This instruction manual is written for users of Nikon Inverted Metallurgical Microscope ECLIPSE MA200.
To ensure correct usage, read this manual carefully before operating the product.
• No part of this manual may be reproduced or transmitted in any form without prior written permission from
Nikon.
• The contents of this manual are subject to change without notice.
• Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may
remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon
representative.
• Some of the equipment described in this manual may not be included in the set you have purchased.
• If you intend to use any other equipment with this product, read the manual for that equipment too.
• If the equipment is used in a manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.
1
Safety Precautions
To ensure correct and safe operation, read this manual before using the product.
Warning and Caution Symbols Used in This Manual
Although this product is designed and manufactured to be completely safe during use, incorrect usage or
failure to follow the safety instructions provided may cause personal injury or property damage. To ensure
correct usage, read this manual carefully before using the product. Do not discard this manual and keep it
handy for easy reference.
Safety instructions in this manual are marked with the following symbols to highlight their importance. For your
safety, always follow the instructions marked with these symbols.
Symbol
Description
Warning
Disregarding instructions marked with this symbol may lead to serious injury or
death.
Caution
Disregarding instructions marked with this symbol may lead to injury or property
damage.
Meaning of Symbols Used on the Product
Symbol
Description
Caution for heat
This marking on the back of the lamphouse calls your attention on the following (the
position of this symbol is shown in Figure 1.1-3):
• The lamphouse becomes extremely hot while the lamp is on and immediately
after it is turned off.
• Do not touch the lamphouse during and immediately after lighting to prevent the
risk of burns.
• Make sure that the lamphouse is sufficiently cool before the lamp replacement.
2
Safety Precautions
WARNING
1.
Intended product use
The product should only be used for microscopic observation. Do not use this microscope for other purposes.
Additionally, do not try to put a large sample on the stage if it is larger than the stage.
2.
Do not disassemble
Disassembling the microscope or the microscope system may result in electric shock or malfunctions.
Damage or injury that may occur due to mishandling is unwarranted. Never attempt to disassemble any part
other than the parts described in this manual. If you experience problems with the microscope or the
microscope system, contact your nearest Nikon representative.
3.
Read the instructions carefully
To ensure safety, carefully read this manual and the manuals for other equipment used with this microscope.
In particular, observe all
warnings and
cautions given at the beginning of each manual.
4.
Ratings of the power supply
The power supply circuit in this product is designed for AC power of 100 to 240 VAC and 50/60 Hz. Before
connecting the power cord, check that the power supply to be used conforms to the voltage and frequency
described above. Use of a non-conforming power line may result in equipment malfunction, failure or fire.
5.
Power cord
Be sure to use the specified power cord for the product. Using a wrong power cord may result in malfunctions
or fire. The product is classified as subject to Class I protection against electrical shock. Make sure it is
connected to an appropriate ground terminal (protective earth terminal). To prevent electrical shock, always
turn off the power switch (set the switch to the “c” position) for the product before connecting or
disconnecting the power cord. For specifications of the power cord, refer to “7. Specifications.”
6.
Specified light source
Use this product with a specified light source. The specified light source devices are shown as follows:
For the episcopic illumination
• Lamphouse
Nikon 12V 50W Precentered Lamphouse (model name: LV-LH50PC)
• Lamp
Nikon 12V 50W longlife halogen lamp (model name: LV-HL50W) or 12V 50W shortlife halogen lamp from
other manufacturers (model name: OSRAM HLX 64610, OSRAM HLX 64611, or PHILIPS 7027).
For the diascopic illumination
• Lamphouse
Nikon 12V 100W Precentered Lamphouse LC (model name: D-LH/LC)
• Lamp
Nikon 12V 100W halogen lamp (model name: OSRAM HLX 64623 or PHILIPS 77241).
• TI-PS 100W power supply
If you wish to buy these lamps, please contact your nearest Nikon representative.
3
Safety Precautions
7.
Heat from the light source
The lamp and the lamphouse become extremely hot. To avoid burns, do not touch the lamphouse while the
lamp is lit or for thirty minutes after it is turned off. Additionally, to avoid the risk of fire, do not place fabric,
paper, or highly flammable volatile materials (such as gasoline, petroleum benzine, paint thinner, or alcohol)
near the lamphouse while the lamp is lit or for about thirty minutes after it is turned off.
8.
Air vents
Do not block the air vents on the product and the lamphouse. If the air vents are blocked, the temperature
inside the product will rise. And it may result in damage or fire.
9.
To use the HG Precentered Fiber Illuminator
To perform the epi-fl microscopy with this product, the brightness of the specified light source may be less
than the desired brightness. In this case, connect an external light source that has a mercury lamp, Nikon
INTENSILIGHT HG Precentered Fiber Illuminator (model: C-HGFI manual type, or C-HGFIE motorized type)
to MA200 to be used.
• Handling the mercury lamp
When using the mercury lamp, you must take great care of the lamp. Read the instruction manual for the
light source and follow the instructions and cautions.
• Ultraviolet light from an external light source
If you use an external light source other than the specified ones and that has a mercury lamp, the light
source radiates ultraviolet light, which is harmful to the eyes and skin, from the emission port. Direct
viewing of light from these lamps may result in snow blindness at a light case or blindness at the worst
case. To prevent injury, be sure to attach a fiber illuminator to the microscope.
The light source device is required to be connected to the microscope whenever the light source device is
energized. Do not turn on the light source if it is not connected to the microscope, and do not disconnect
the light source from the microscope while the light source is lit. When disconnecting the light source from
the microscope, turn off the power to the light source, and then unplug the power cord from the outlet.
4
Safety Precautions
CAUTION
1.
Handle with care
This product is a precision optical instrument. Handle the microscope system with care to avoid shock on
impact. In particular, objectives may loose accuracy when exposed to even a weak physical shock.
2.
Do not wet the microscope
If the product gets wet, a short circuit may cause malfunction or abnormal heating of the microscope. If you
accidentally spill water on the microscope, immediately turn off the power switch (set the switch to the “c”
side) and unplug the power cord from the outlet. Then, wipe off the water with a piece of dry cloth. If water
enters a component, immediately suspend use of this product, disconnect the power cord from the outlet, and
contact your nearest Nikon representative.
3.
Weak electromagnetic waves
The product emits weak electromagnetic waves. The accuracy of any precision electronic equipment may be
adversely affected if positioned too close. To prevent bad influences, locate such electronic equipment away
from the microscope system. If a TV or radio reception is affected, move the TV or radio set farther from the
product.
4.
Installation location
This product is a precision optical instrument. The usage or storage in an inappropriate environment may
result in malfunctions or poor performance. Consider the following factors when selecting an installation
location:
• Select an installation location with a temperature from 0 to +40°C and a relative humidity of 85% or less
(there should be no condensation).
Select a storage location with a temperature from -20 to +60°C and a relative humidity of 90% or less
(there should be no condensation).
If installed or stored in a location subject to high temperatures and humidity, mold or condensation may
form on the lens, resulting in lowered performance and possible damage to the microscope.
• Avoid a brightly lit location, such as exposed to direct sunlight or directly under a room light. If there is
excessive ambient light, the image may not clearly be visible.
• Always install the product with a surrounding clear area of 10 cm or more.
• Install the product in a location free from considerable dust or dirt.
• Install the product on a flat surface with little vibration.
• Install the product on a sturdy desk or table for the base of the microscope system.
• Select a layout that allows easy removal of the power cord from the AC inlet of the product in the event of
an emergency.
• Do not install in a narrow space such as a shelf or locker.
• Do not place anything on the product.
• Cover the product to avoid dust when storing.
• For details about the operating environment and storage environment, refer to “7. Specifications.”
5.
Cautions on moving the microscope
• This product is a precision optical instrument. Handle it carefully and do not subject it to a strong physical
shock. (In particular, objectives may loose accuracy when exposed to even a weak physical shock.)
• Securely hold the microscope at the front bottom and rear bottom when carrying it.
5
Safety Precautions
• Do not hold the focus knobs, eyepiece tube, lamphouse, stage, and so on, when carrying the microscope.
They may come off and may cause serious injury or malfunction.
• Be careful not to pinch your hands or fingers during transportation.
6.
Cautions on assembling the microscope
• Be careful not to pinch your fingers or hands during assembly.
• Scratches or fingerprints on the optical components, including lenses or filters etc., will adversely affect the
image. Be careful not to scratch or touch the lens surfaces.
7.
Cautions on replacing lamps
• To prevent burn injuries, wait at least 30 minutes after the lamp is turned off to give it sufficient time to cool
down when replacing lamps.
• To prevent electrical shock and damage to the microscope, always turn off the power switch (set the switch
to the “c” side) and unplug the power cord from the outlet before attaching or detaching the lamphouse.
• Never touch the glass surface of the lamp with bare hands. Doing so may cause fingerprints, grease, etc.,
to generate ghost images on the lamp surface, reducing the illumination. If you do get any fingerprints or
dirt on the lamp, wipe them clean.
• Make sure the lamphouse cover is securely fitted to the lamphouse after replacing lamps. Never turn on
the lamp with the lamphouse cover removed.
• When you dispose of the replaced lamp, do not break it. Instead, dispose of the used lamp as industrial
waste or dispose of it according to the local regulations and rules.
8.
Cable routing
Make sure the cables are routed properly. Do not bring the cables into contact with the lamphouse. If a cable
comes into contact with the lamphouse, the cable sheath may melt and it may result in an electrical shock or
fire. Additionally, connect the cables by placing them into the cable keeper on the rear of the microscope main
body.
6
Contents
Introduction ....................................................................................................................................................................... 1
Safety Precautions............................................................................................................................................................ 2
Warning and Caution Symbols Used in This Manual ................................................................................................. 2
Meaning of Symbols Used on the Product ................................................................................................................. 2
WARNING .......................................................................................................................................................... 3
CAUTION ........................................................................................................................................................... 5
1.
Part Name and Function ........................................................................................................................................ 10
2.
Microscopy ............................................................................................................................................................. 14
2.1
3.
Bright-field Microscopy under the Episcopic Illumination .............................................................................. 16
2.2
Dark-field Microscopy under the Episcopic Illumination ................................................................................ 19
2.3
Polarization Microscopy under the Episcopic Illumination (simplified/sensitive color)................................... 20
2.4
Differential Interference Contrast Microscopy under the Episcopic Illumination............................................ 21
2.5
Epi-fl Microscopy........................................................................................................................................... 22
2.6
Bright-field Microscopy under the Diascopic Illumination .............................................................................. 24
2.7
Polarization Microscopy under the Diascopic Illumination (simplified/sensitive color)................................... 28
Operation Details.................................................................................................................................................... 29
3.1
3.2
Power ON/OFF ............................................................................................................................................. 29
3.1.1
Power of the microscope............................................................................................................... 29
3.1.2
Power supply of the lamp .............................................................................................................. 29
Illumination.................................................................................................................................................... 30
3.2.1
Brightness control and illumination ON/OFF ................................................................................. 30
3.2.2
Switching the Internal/External brightness control......................................................................... 30
3.2.3
Displaying the POWER LED ......................................................................................................... 31
3.3
Selecting the Microscopy Method ................................................................................................................. 31
3.4
Eyepiece Tube .............................................................................................................................................. 33
3.4.1
Selecting optical path .................................................................................................................... 33
3.4.2
Adjusting the eyelevel risers.......................................................................................................... 34
3.5
Adjusting the Interpupillary Distance............................................................................................................. 34
3.6
Adjusting the Diopters................................................................................................................................... 35
3.7
Adjusting the Focus (for focus operation) ..................................................................................................... 36
3.8
3.9
3.7.1
Using the coarse/fine focus knob .................................................................................................. 36
3.7.2
Adjusting the torque for the coarse focus ring ............................................................................... 36
Placing the Sample and Operating the Stage ............................................................................................... 37
3.8.1
Placing the sample........................................................................................................................ 37
3.8.2
Changing the Observation Position............................................................................................... 38
Operating the Revolving Nosepiece and the Objective................................................................................. 39
3.9.1
Revolving nosepiece in combination with the objective................................................................. 39
3.9.2
Changing the objectives ................................................................................................................ 39
3.9.3
Displaying the address for the revolving nosepiece ...................................................................... 39
3.10
Filter.............................................................................................................................................................. 40
3.11
Adjusting the Field Diaphragm (for the episcopic Illumination) ..................................................................... 41
3.12
Adjusting the Aperture Diaphragm (for the episcopic illumination) ................................................................ 42
3.13
Using the Polarizer/Analyzer Unit (MA2-PA/MA2-UPA) ................................................................................ 43
3.13.1
Inserting/removing the polarizer/analyzer from the optical path .................................................... 43
7
Contents
3.13.2
3.14
Using the λ Plate (MA2-λP)........................................................................................................................... 45
3.14.1
3.15
3.16
3.17
3.15.1
Inserting/removing the DIC prism from the optical path ................................................................ 46
3.15.2
Setting the DIC prism .................................................................................................................... 46
3.15.3
Interference color .......................................................................................................................... 47
Using the DIC Slider (LV-DIHC/LV-DIC) ........................................................................................................ 47
3.16.1
Selecting the DIC slider................................................................................................................. 47
3.16.2
Inserting/removing the DIC slider from the optical path................................................................. 47
3.16.3
Interference color .......................................................................................................................... 48
Using the Analyzer Slider (D-DA).................................................................................................................. 48
3.20
Inserting/removing the λ plate from the optical path...................................................................... 49
Using the Fluorescent Unit (MA2-FL)............................................................................................................ 50
3.19.1
Inserting/removing the fl filter from the optical path....................................................................... 50
3.19.2
Excitation light filter (EX filter) ....................................................................................................... 51
3.19.3
Barrier filter (BA filter).................................................................................................................... 52
Using the Scale Slider (MA2-GR/MA2-MR) .................................................................................................. 53
3.20.1
Grain scale slider (MA2-GR) ......................................................................................................... 53
3.20.2
Scale slider (MA2-MR) .................................................................................................................. 54
3.21
Using the Intermediate Magnification Unit (MA2-MC) ................................................................................... 55
3.22
Using the HG Precentered Fiber Illuminator (C-HGFI/C-HGFIE) .................................................................. 56
3.22.1
3.23
4.
Inserting/removing the analyzer from the optical path................................................................... 48
Using the λ Plate (D-LP) ............................................................................................................................... 49
3.18.1
3.19
Inserting/removing the λ plate ....................................................................................................... 45
Using the DIC Slider (L-DIHC/L-DIC)............................................................................................................ 46
3.17.1
3.18
Adjusting the polarizer direction .................................................................................................... 44
Procedure for turning on the power switch .................................................................................... 56
Using the supporting pillar for dia-Illuminator 100W (MA2-DP)..................................................................... 57
3.23.1
Procedure for turning on the power switch .................................................................................... 57
3.23.2
Focusing and centering the condenser ......................................................................................... 58
3.23.3
Adjusting the field diaphragm (for the diascopic illumination)........................................................ 59
3.23.4
Adjusting the aperture diaphragm (for the diascopic illumination) ................................................. 60
3.23.5
Diascopic illumination filter ............................................................................................................ 60
3.23.6
Condenser refocusing clamp......................................................................................................... 61
3.23.7
Condenser mount rotation............................................................................................................. 61
3.23.8
Polarizer slider (T-P2), λ plate (TI-DIC) ......................................................................................... 62
3.24
Using the Supporting Arm (MA2-MP)............................................................................................................ 64
3.25
Using the DS Camera Control Unit (DS-L2).................................................................................................. 65
3.25.1
Procedure for turning on the power............................................................................................... 65
3.25.2
Displaying the microscope info menu............................................................................................ 65
3.25.3
Measurement corresponding to the magnification value of the microscope .................................. 66
3.25.4
Recording the microscope info while saving the image................................................................. 66
3.25.5
Connecting with the DS camera head switcher (DS-SW: Camera switch BOX, optional) ............. 67
Assembly ................................................................................................................................................................ 68
4.1
About the System.......................................................................................................................................... 70
4.2
Combination List for the Unit......................................................................................................................... 71
8
Contents
4.3
4.4
4.5
4.6
Network Connection...................................................................................................................................... 73
4.3.1
Unit system capable of establishing network................................................................................. 73
4.3.2
Viewing user control options and status displays on a PC or the DS-L2 ....................................... 74
4.3.3
Registering information on the objective ....................................................................................... 76
4.3.4
Procedure for turning on the power switch .................................................................................... 76
The LV-LH50PC Lamphouse and the Lamp.................................................................................................. 77
4.4.1
Attaching the lamphouse............................................................................................................... 77
4.4.2
Replacing the lamp ....................................................................................................................... 78
Revolving Nosepiece .................................................................................................................................... 78
4.5.1
Attaching the nosepiece ................................................................................................................ 78
4.5.2
Cable connection for the manual revolving nosepiece and the MA200......................................... 79
4.5.3
Connecting the motorized nosepiece and the Nosepiece Controller 2.......................................... 79
Stage............................................................................................................................................................. 80
4.6.1
4.7
Objectives ..................................................................................................................................................... 81
4.8
Eyepiece Tube .............................................................................................................................................. 81
4.9
Eyepieces ..................................................................................................................................................... 81
4.10
Grain Scale Slider/Scale Slider ..................................................................................................................... 82
4.11
Filters ............................................................................................................................................................ 82
4.12
Various Sliders Available for Attaching the Revolving Nosepiece ................................................................. 83
4.13
4.12.1
L-DIC/L-DIHC slider (single NR method) ...................................................................................... 83
4.12.2
LV-DIC/LV-DIHC slider (Senarmont method)................................................................................. 84
4.12.3
D-DA analyzer slider, D-LP λ plate ................................................................................................ 84
Polarizer/analyzer Unit and Fluorescent Unit ................................................................................................ 85
4.13.1
5.
6.
7.
Sample holder ............................................................................................................................... 80
MA2-λP λ plate.............................................................................................................................. 85
4.14
Intermediate Magnification Unit..................................................................................................................... 86
4.15
External Light Source.................................................................................................................................... 87
4.15.1
C-HGFI/C-HGFIE HG Precentered Fiber Illuminator..................................................................... 87
4.15.2
Attaching the MA2-DP supporting pillar for dia-Illuminator 100W.................................................. 88
4.16
Supporting Arm (for DS-L2) .......................................................................................................................... 94
4.17
Eyelevel Risers ............................................................................................................................................. 96
4.18
Camera Adapter............................................................................................................................................ 97
4.19
Power Cord ................................................................................................................................................... 97
Troubleshooting ..................................................................................................................................................... 98
5.1
Viewing Problems and Control Problems...................................................................................................... 98
5.2
Electrical System Problems ........................................................................................................................ 101
Care and Maintenance ......................................................................................................................................... 102
6.1
Cleaning the Lenses and Filters.................................................................................................................. 102
6.2
Cleaning the Painted Parts, Plastic Parts, and Printed Parts ...................................................................... 102
6.3
Storage ....................................................................................................................................................... 103
6.4
Regular Inspections (fee charged) .............................................................................................................. 103
Specifications....................................................................................................................................................... 104
9
1
Part Name and Function
Eyepiece
Sample holder
10X, 12.5X, 15X (and 10X
are equipped with the
mask eyepiece.)
Eyepiece tube
(Figure illustrates
the MA2-TI3.)
The stage is equipped with the standard
sample holder (with a sample clip). (Refer to
3.8.)
Sample clip
Binocular eyepiece
may also be used.
(Refer to 3.4, 3.5,
and 3.6.)
Stage (Figure illustrates the MA2-SR.)
(Refer to 3.8.)
Objectives
(Refer to 3.9.)
Nosepiece (Figure illustrates the
MA2-NUI5.)
Quintuple, sextuple and septuple
revolving nosepieces may be used.
(Refer to 3.9.)
The DIC slider, analyzer slider etc.,
can be attached, depending on the
revolving nosepiece used.
Stage (forward/backward)
movement knob for the Y
direction
Stage (left /right) movement
knob for the X direction
Display
Fine focus knob
(Refer to Figure
1.1-2.)
(Refer to 3.7.) Located also on the left.
Front-cover fixing bolt
Aperture
diaphragm dial
(Refer to 3.12.)
BD field changeover lever
Press (BF): Bright-field
Pull (DF): Dark-field (Refer to 3.3.)
Scale slider slot
(Refer to 3.20.)
Attach the grain
scale slider or scale
slider.
Aperture diaphragm
centering holes
Field diaphragm
dial
(Refer to 3.11.)
Operation port for the polarizer/analyzer unit
and the fluorescent unit
(Refer to 3.13 and 3.19.)
To perform the polarization microscopy, differential
interference contrast microscopy or fluorescent
microscopy, remove the front cover to attach the
desired unit for the microscopy to be performed.
Field diaphragm
centering holes
Figure 1.1-1 Front view, Right-side view
10
Chapter 1
Part Name and Function
POWER LED
Displays ON/OFF for the power switch or the light-status for the illumination
lamp. Additionally, the display status remains the same when an external
PC is in use.
Power switch OFF: LED turns off
Power switch ON; Brightness control dial OFF: Orange LED lights up
Power switch ON; Brightness control dial ON: Green LED lights up
Note: The Power LED Message is only compatible with the lamphouse of
the MA200 main body for the episcopic illumination, and the HG
precentered fiber illuminator (C-HGFIE motorized type).
Address display
Address of the objective in the optical path lights up.
Figure 1.1-2 Front view
11
Display
Chapter 1
Part Name and Function
Supporting Pillar for Dia-Illuminator
100W/Supporting Arm for DS-L2
attaching part
Optical path changeover
lever (eyepiece tube/back
port)
(Refer to 3.23 and 3.24.)
Attach the supporting pillar for
dia-Illuminator 100W or the supporting
arm for DS-L2.
(Refer to 3.4.)
Press: Eyepiece tube/back
port =100/0
Tool storage
Stores the supplied hexagonal
wrench and two hexagonal
screwdrivers.
Rear-panel clamp screw
Rear-panel
Eyepiece tube clamp screw
Pull: Eyepiece tube/back port
=55/45
Attaching part for the
intermediate magnification
unit
(Refer to 3.21.)
Attach this unit to change
observation magnifications.
Remove the rear-panel
cover for cable connection
of the revolving nosepiece.
Optical path changeover lever
(vertical tube/binocular tube)
(Refer to 3.4.)
Press: Binocular/vertical tube=100/0
Pull: Binocular/vertical tube=0/100
Vertical
tube
Binocular
tube
Back port
Air vents
Cable keeper
Keeps cables inside.
“CAUTION for heat”
symbol
Clamp
screws for
the filter
turret
Epi illumination
lamphouse
(LV-LH50PC Lamphouse)
Attach an HG precentered
fiber illuminator instead of
LV-LH 50PC Lamphouse,
if necessary.
Signal cable hole for the
nosepiece controller 2
Connectors
Brightness control
dial
Lamp air
vents
(Refer to 3.2.)
Adjusts the brightness
of the lamp.
(Refer to Figure 1.1-4.)
AC inlet
Connects the power cord exclusive
for the region of the product used
(optional).
Power switch
Fine focus knob
Filter turret
(Refer to 3.10.)
Switch the turret when the ND
filter or other filters is/are turned.
(Refer to 3.1.)
Set the switch to the “|” side to turn
on the power, or to the “c” side to
turn off the power.
Coarse torque
adjustment ring
(Refer to 3.7.)
Adjusts the rotation
residence.
When the power is turned on, the
POWER LED on the front-display
lights up.
Figure 1.1-3 Left view, Rear view
12
(Refer to 3.7.)
Focus on the sample by
slightly lifting/lowering the
revolving nosepiece with
the fine knob.
Coarse focus knob
(Refer to 3.7.)
Focus on the sample roughly
lifting/lowering the revolving
nosepiece with the coarse
knob.
Chapter 1
Part Name and Function
Lamphouse connector
[LAMP DC12V50W]
Connects the cord on the epi
illumination lamphouse (12V
50W Precentered Lamphouse).
Internal/External brightness
control changeover switch
[EXTERNAL ON/OFF]
RS232C connector [HGFIE]
Connects the C-HGFIE HG
Precentered Fiber Illuminator.
(Refer to 3.2.2, 3.22.1, and
3.23.1.)
ON: Adjusts the brightness on
PC;
RS232C connector [NCNT]
OFF: Adjusts the brightness on
the microscope.
Connects the LV-NCNT2
control unit for the motorized
nosepiece.
USB connector [USB]
Connects a PC or the DS-L2.
Figure 1.1-4 Connectors on the rear
About product parts names in this manual
The right column of the table below shows the product parts names described in this manual.
Package names
Parts names in the ECLIPSE MA200 Instructions
MA2-TI3 Trinocular Eyepiece Tube ESD (erect image)
MA2-TI3 Trinocular Eyepiece Tube
MA2-NUI5 Intelligent Universal Quintuple
Nosepiece ESD
MA2-NUI5 Revolving Nosepiece
MA2-MC Magnification Module
MA2-MC Intermediate Magnification Unit
Fl Filter Block MA2-FL G
(Other types than G are available.)
General name: MA2-FL Fluorescent Unit
MA2-GR Grain Size Slider
MA2-GR Grain Scale Slider
MA2-PA Polarizing Filter Cube
MA2-PA Polarizer/Analyzer Unit
MA2-UPA Polarizing Filter Cube with 1/4λPlate
MA2-UPA Polarizer/Analyzer Unit
MA2-SRSH 10 Specimen Holder
(Other types than SRSH 10 are available.)
General name: Sample Holder
13
2
Microscopy
This chapter describes the procedure of each microscopy.
See Table 2.1 for the items required for each microscopy.
• Refer to “4. Assembly,” when the product has not been assembled yet.
• For detailed information about operations of parts of the product, refer to “3. Operation Details.”
• Notes on using an external light source:
The procedures described on the episcopic illumination in 2.1 to 2.5 are based on use of the light source of
the MA200 main body with the LV-LH50PC Lamphouse attached. When using an external light source to
perform the microscopy, follow the notes below.
• The procedure on how to turn the power switch to ON: Turn on the power switch on the external device
in advance, then turn on the MA200 main body.
• Setting the Internal/External brightness control changeover switch of the MA200 main body: Sets to ON
(external mode).
• Adjusting the brightness of the light source: Adjusts the brightness on the external light source.
• To perform the epi-fl microscopy using the C-HGFI/C-HGFIE HG Precentered Fiber Illuminator as the
external light source, screw the compensation filter supplied with the fiber adapter into the MA2-FL
fluorescent unit. (Refer to 4.15.1.)
14
Chapter 2
Table 2.1
Microscopy
BF microscopy
under the epi
illumination
Page
Illuminator
p.16 to p.18
Microscopy
Items required for the microscopy
Revolving nosepiece
5 revo., 6 revo.,
7 revo.
Objective
Other items required
• For EPI objectives with 5
revo., LU nosepiece
adapter M32-25 required
⎯
• For 6 or 7 revo., BD
objective unavailable
DF microscopy
under the epi
illumination
p.19
Simplified
polarization
microscopy
under the epi
illumination
p.20
5 revo. only
BD objective only
⎯
5 revo., 6 revo., 7
revo.
• For EPI objectives with 5
revo., LU nosepiece
adapter M32-25 required
• For 6 or 7 revo., BD
objective unavailable
DIC microscopy p.21 to p.22
under the epi
illumination
5 revo., 6 revo.
LU EPI objectives or LU BD
objective only
• For EPI objectives with 5
revo., LU nosepiece
adapter M32-25 required
LV-LH 50PC
Lamphouse or
C-HGFI/C-HGFIE
HG Precentered
Fiber Illuminator
• BD objective unavailable
for 6 revo.
• MA2-PA/MA2-UPA
polarizer/analyzer unit
• For the sensitive color
polarization microscopy,
MA2-λP λ plate available
For 5 revo.:
• MA2-PA
polarizer/analyzer unit
• L-DIHC/L-DIC slider
(single NR method)
• For the sensitive color
polarization microscopy,
the MA2-λP λplate
available
For 6 revo.:
• MA2-UPA
polarizer/analyzer unit
• LV-DIHC/LV-DIC slider
(Senarmont method)
• For the sensitive color
polarization microscopy,
the MA2-λP λplate
available
Epi-fl
microscopy
p.22
5 revo., 6 revo., 7
revo.
LU EPI objectives or LU BD
objective only
• MA2-FL fluorescent unit
• For EPI objectives with 5
revo., LU nosepiece
adapter M32-25 required
• For 6 or 7 revo., BD
objective unavailable
BF microscopy
under the dia
illumination
Simplified
polarization
microscopy
under the dia
illumination
p.23 to p.26
p.27
5 revo., 6 revo., 7
revo.
Supporting pillar
for dia-Illuminator
100W
(+ condenser,
D-LH/LC
Lamphouse, 12V
100W halogen
lamp)
• For EPI objectives with 5
revo., LU nosepiece
adapter M32-25 required
⎯
• For 6 or 7 revo., BD
objective unavailable
5 revo., 6 revo., 7
revo.
• For EPI objectives with 5
revo., LU nosepiece
adapter M32-25 required
• For 6 or 7 revo., BD
objective unavailable
• T-P2 polarizer
• MA2-PA/MA2-UPA
polarizer/analyzer unit (for
6 revo., the D-DA analyzer
is also available)
• For the sensitive color
polarization microscopy,
TI-DIC λplate available
(for 6 revo., the D-LP
λplate is also available)
15
Chapter 2
2.1
Microscopy
Bright-field Microscopy under the Episcopic Illumination
Turn on the power to light up the episcopic illumination lamp.
1 Turn on the power switch. (See 3.1.1.)
The power LED on the front is lit.
2 Be sure that the Internal/External brightness
control changeover switch is set to OFF “internal
mode.” (See 3.2.2.)
3 Turn the brightness control dial to light up the
lamp. (See 3.2.1.)
1-2
1-1
1-3
1-1
16
Chapter 2
Microscopy
Set the microscope for the bright-field microscopy under the episcopic illumination.
If the current unit is the one shown in the column at the right edge under “Other items required” in Table 2.1,
pull out each item from the optical path.
1 Push in the optical path changeover lever for the
eyepiece tube and select 100% for the binocular
eyepiece. (See 3.4.1.)
2-4
2-1
2 Push in the eyepiece tube/back port changeover
lever to select 100% for the eyepiece tube. (See
3.4.1.)
2-2
2-5, 6
3 Push in the BD field changeover lever to select the
“BF (bright-field)” position. (See 3.3.)
2-4
4 Turn the revolving nosepiece to place the 10X
objective into the optical path. (See 3.9.2)
The nosepiece addresses (1 to 7) on the
front-display light up to indicate the position of the
revolving nosepiece. (See 3.9.3.)
2-6
5 Place the NCB11 filter in the filter turret into the
optical path and compensate color temperature.
(See 3.10)
2-3
2-7
6 Adjust the brightness roughly with the brightness
control dial and the desired ND filter in the filter
turret. (See 3.2.1 and 3.10.)
7 Turn the field diaphragm dial and the aperture
diaphragm dial counter-clockwise to the limit to
fully open the field diaphragm and the aperture
diaphragm for the episcopic illumination. (See 3.11
and 3.12.)
Place the sample onto the stage and adjust the focus and the brightness.
1 Use the sample holder according to the selected
sample. Place the sample onto the stage and
move the stage using the stage movement knob
for the X/Y direction so that the observation
position comes to the center of the view-field. (See
3.8.)
3-1
2 Turn the coarse/fine focus knobs and focus on the
target. (See 3.7.)
3 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
3-2
3-3
3-1
Adjust the diopter and the interpupillary distance.
1 Adjust the interpupillary distance. (See 3.5.)
2 Adjust the diopter. (See 3.6.)
17
Chapter 2
Microscopy
Set the desired magnification and observe the sample.
5-1
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Turn the coarse/fine focus knobs and refocus on
the sample. (See 3.7.)
5-6
3 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
4 Turn the field diaphragm dial so that the field
diaphragm image circumscribes the view-field.
(See 3.11.)
5-2
5-3
5-5
5-4
5 Turn the aperture diaphragm dial so that the
aperture diaphragm image becomes 70 to 80% of
the numerical aperture of the objective. (See
3.12.)
6 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
Note: Adjusting focus
It may be difficult to focus on a sample with small contrast, such on a polished surface. In a case like
this, stop down the field diaphragm so that its image can be seen in the view-field, and try to focus on
the frame of the diaphragm image. When the frame is in focus, the sample is in focus just as well.
18
Chapter 2
2.2
Microscopy
Dark-field Microscopy under the Episcopic Illumination
Attach the items required for the dark-field microscopy under the episcopic illumination
to the microscope. (See Table 2.1.)
Focus on the sample with the bright-field microscopy under the episcopic illumination.
(See Pages 16 and 17.)
Set the microscope for the dark-field microscopy under the episcopic illumination.
3-1
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Pull out the BD field changeover lever to select the
“DF (dark-field)” position. (See 3.3.)
3-4
The aperture diaphragm and the field diaphragm
are fully opened. (The dial positions do not
change.)
When the polarizer/analyzer or the fl filter is
placed into the optical path, it is automatically
excluded.
3-3
3 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
3-2
4 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
Return to the bright-field microscopy under the episcopic illumination.
1 Push in the BD field changeover lever to select the
“BF (bright-field)” position. (See 3.3.)
The aperture diaphragm and the field diaphragm
automatically return to the previous positions.
(The dial positions do not change.)
2 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1)
3 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
19
Chapter 2
2.3
Microscopy
Polarization Microscopy under the Episcopic Illumination (simplified/sensitive color)
Attach the items required for the polarization microscopy under the episcopic
illumination to the microscope. (See Table 2.1.)
Focus on the sample with the bright-field microscopy under the episcopic illumination.
(See Pages 16 to 17.)
Set the microscope for the polarization microscopy under the episcopic illumination.
3-1
3-4
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Push in the polarizer/analyzer unit to the second
click-stop position and place it into the optical
path. (See 3.13.1.)
3-6
3 Turn the polarizer rotation ring to the crossed
Nicol’s position. (See 3.13.2.)
4 To perform the sensitive color polarization
microscopy, insert the λplate into the
polarizer/analyzer unit and place it into the optical
path. (See 3.14.1.)
3-5
3-3, 7
3-2
5 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
6 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
7 Turn the polarizer rotation ring to adjust the
polarization while observing the image.
Return to the bright-field microscopy under the episcopic illumination.
1 Pull the analyzer/polarizer unit to the first
click-stop position to remove the
analyzer/polarizer from the optical path. (See
3.13.1.)
2 Both the λplate and the polarizer/analyzer unit are
removed from the optical path. (See 3.14.1.)
3 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
4 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
20
Chapter 2
2.4
Microscopy
Differential Interference Contrast Microscopy under the Episcopic Illumination
Attach the items required for the differential interference contrast (DIC) microscopy
under the episcopic illumination to the microscope. (See Table 2.1.)
Focus on the sample with the bright-field microscopy under the episcopic illumination.
(See Pages 16 and 17.)
Set the microscope for the DIC microscopy under the episcopic illumination.
3-5
3-1
3-4
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Push in the polarizer/analyzer unit to the second
click-stop position and place it into the optical
path. (See 3.13.1.)
3-7
3 Turn the polarizer rotation ring to the crossed
Nicol’s position (See 3.13.2.)
4 Attach the DIC slider to the slot on the revolving
nosepiece to place the DIC prism into the optical
path. (See 3.15.1 or 3.16.2.)
3-6
3-3, 5
3-2
5 Set the interference color. (See 3.15.3 or 3.16.3.)
For using the L-DIHC/L-DIC slider, turn the prism
movement knob, whereas for using the
LV-DIHC/LV-DIC slider, turn the polarizer rotation
ring of the polarizer/analyzer unit. In either case,
the interference color changes continuously from
dark color, gray to sensitive red-violet.
6 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
7 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
■ Sensitive color microscopy
You can perform the sensitive color DIC microscopy using the λ plate placed into the polarizer/analyzer unit.
If the λ plate is placed into the optical path in the crossed Nicol’s position (on dark background), the
background is set to sensitive red-violet. This improves the contrast of the image to the highest degree. If you
turn the polarizer rotation ring to pale-blue for background change when the λ plate is inserted, the
interference image will resemble the dark contrast image in the phase contrast microscopy. Select the
background color to achieve the desired contrast, if the phase contrast ratio varies intensively (for the rough,
uneven surface of sample).
21
Chapter 2
Microscopy
Return to the bright-field microscopy under the episcopic illumination.
1 Pull the polarizer/analyzer unit to the first
stop-click position and remove the
polarizer/analyzer from the optical path. (See
3.13.1.)
2 Both the λplate and the polarizer/analyzer unit are
removed from the optical path. (See 3.14.1.)
3 Detach the DIC slider to remove the DIC prism
from the optical path. (See 3.15.1 or 3.16.2.)
4 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
5 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
2.5
Epi-fl Microscopy
Attach the items required for the epi-fl microscopy to the microscope. (See Table 2.1.)
To perform the epi-fl microscopy using the C-HGFI/C-HGFIE HG Precentered Fiber Illuminator as the
external light source, screw the compensation filter supplied with the fiber adapter into the MA2-FL
fluorescent unit. (Refer to 4.15.1.)
Find the target and focus on the sample by BF/DF microscopy under the episcopic
illumination. (See Pages 16 to 17, and 19.)
Set the microscope for the epi-fl microscopy.
3-1
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Turn the BD field changeover lever to the “BF
(bright-field)” position. (See 3.3.)
3 Push the attached fluorescent unit into the second
click-stop position and place the fl filter into the
optical path (See 3.19.1.)
3-5
4 Turn the brightness control dial to adjust the
brightness. (See 3.2.1.)
5 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
3-4
3-3
3-2
22
Chapter 2
Microscopy
Return to the bright-field or dark-field microscopy under the episcopic illumination.
1 Turn the BD field changeover lever to the “BF
(bright-field)” or “DF (dark-field)” position. (See
3.3.)
2 Turn the brightness control dial to adjust the
brightness. (See 3.2.1.)
3 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
23
Chapter 2
2.6
Microscopy
Bright-field Microscopy under the Diascopic Illumination
Attach the items required for the bright-field microscopy under the diascopic illumination.
(See Table 2.1.)
Turn on the power to light up the diascopic illumination lamp. (See 3.23.1.)
1 Set the brightness control dial to OFF on the
MA200 main body. (See 3.2.1.)
2 Turn on the TI-PS100W power supply connected
to the supporting pillar for dia-Illuminator.
3 Set the MA200 main body to ON.
The power LED on the front is lit.
The light intensity control dial for the TI-PS100W
power supply is enabled.
2-4
4 Turn the light intensity control dial on the
TI-PS100W power supply to adjust the
illumination.
You can change the output voltage within 1V to
12V. When the output voltage is set to around 9V,
the ideal optical color reproduction generates.
2-2
2-3
2-1
2-3
24
Chapter 2
Microscopy
Set the microscope for the bright-field microscopy under the diascopic illumination.
When the “Other items required” (See the column at the right edge of Table 2.1.) is used for the episcopic
illumination, remove each attachment from the optical path in advance.
1 Push in the optical path changeover lever for the
eyepiece tube and select 100% for the binocular
eyepiece. (See 3.4.1.)
2 Push in the BD field changeover lever to select the
“BF (bright-field)” position. (See 3.3.)
3-4, 5
3-6
3 Turn the revolving nosepiece to place the 10X
objective into the optical path. (See 3.9.2.)
3-8
3-1
3-7
3-9
3-5
3-3
3-2
4 Place the NCB filter on the supporting pillar for
dia-Illuminator into the optical path and
compensate the color temperature. (See 3.23.5.)
5 Adjust the brightness of the diascopic illumination
roughly with the light intensity control dial for the
TI-PS100W power supply and the desired ND filter
on the pillar illuminator. (See 3.23.1 and 3.23.5.)
6 Turn the field diaphragm dial for the supporting
pillar for dia-Illuminator clockwise to the limit and
fully open the field diaphragm for the diascopic
illumination. (See 3.23.3.)
7 Turn the aperture diaphragm lever for the system
condenser clockwise to the limit and fully open the
field diaphragm. (See 3.23.4)
8 Turn the condenser focus knobs for the supporting
pillar for dia-Illuminator and lower the condenser
mount to the limit. (See 3.23.6.)
• When the ELWD condenser lens is attached to
the system condenser, lower the condenser
mount approximately 1 cm from the upper limit.
• When the ELWD-S condenser is used, lower
the condenser mount approximately 2 cm from
the upper limit.
9 Turn the turret of the condenser to the “A” position
to place the bright-field condenser cassette into
the optical path.
25
Chapter 2
Microscopy
Place the sample onto the stage to adjust the focus.
1 Use the sample holder according to the selected
sample. Place the sample onto the stage and
move the stage using the stage movement knob
for the X/Y direction so that the observation
position comes to the center of the view-field. (See
3.8.)
4-2
4-1
2 Turn the coarse/fine focus knobs and focus on the
sample. (See 3.7.)
4-1
Adjust the diopter and the interpupillary distance.
1 Adjust the interpupillary distance. (See 3.5.)
2 Adjust the diopter. (See 3.6.)
Adjust the focus again.
1 Look into the eyepieces. Move the stage to bring
the observation target into the center of the
view-field using the stage movement knob for the
X/Y direction.
2 Look into the eyepieces. Adjust the focus onto the
sample by turning the coarse/fine focus knob.
6-1, 2
6-1
6-2
26
Chapter 2
Microscopy
Center the condenser. (See 3.23.2.)
1 Be sure that the objective is set to the 10X
objective.
If not, turn the revolving nosepiece to place the 10X
objective into optical path.
7-2, 6
2 Stop down the field diaphragm by turning the field
diaphragm dial on the supporting pillar for
dia-Illuminator until the field diaphragm image
comes into the view-field.
7-3
7-4, 7
3 Turn the condenser focus knob for the supporting
pillar for dia-Illuminator to focus on the field
diaphragm image.
4 Turn the two condenser centering screws for the
pillar illuminator to move the field diaphragm
images to the center of the view-field.
5 Turn the revolving nosepiece to place the 40X
objective into the optical path.
7-1, 5
6 Turn the field diaphragm dial for the supporting
pillar for dia-Illuminator until the diaphragm image
becomes nearly the same as the view-field.
7 Turn the two condenser centering screws on the
supporting pillar for dia-Illuminator to move the field
diaphragm images to the center of the view-field.
Set the desired magnification and observe the sample.
1 Turn the revolving nosepiece to place the objective
of a desired magnification into the optical path.
8-5
2 Move the aperture diaphragm lever on the system
condenser to adjust the aperture diaphragm to a
size of 70 to 80% of the objective N.A. (See
3.23.4.)
8-4
8-3
3 Turn the field diaphragm dial for the supporting
pillar for dia-Illuminator until the diaphragm
becomes nearly the same as the view-field. (See
3.23.3.)
8-2
8-4
4 Push in or pull out the desired ND filter on the
supporting pillar for dia-Illuminator to place it into
the optical path, then adjust the brightness for the
view-field. (See 3.23.5.)
If accurate color reproduction is not crucial (e.g., for
color photography), change the lamp voltage with
the light intensity control dial on the TI-PS100W
power supply.
8-1
8-5
8-6
5 Look into the eyepieces. Move the stage to bring
the observation target into the center of the
view-field.
6 Turn the coarse/fine focus knobs and focus on the
sample.
Note: Condenser refocusing clamp (See 3.23.6.)
To raise or lower the condenser, secure the clamp in advance. You can restore the condenser position
easily.
27
Chapter 2
2.7
Microscopy
Polarization Microscopy under the Diascopic Illumination (simplified/sensitive color)
Attach the items required for the polarization microscopy under the diascopic
illumination to the microscope. (See Table 2.1.)
Focus on the sample with the bright-field microscopy under the diascopic illumination
and center the condenser. (See pages 23 to 26.)
Set the microscope for the polarization microscopy under the diascopic illumination.
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
3-6
2 Push the polarizer/analyzer unit attached to the
MA200 main body into the optical path. (See
3.13.1.)
3-3, 4
When using the sextuple revolving nosepiece,
insert the D-DA analyzer slider into the slot on the
revolving nosepiece instead of the
polarizer/analyzer unit, if desired. (See 3.17.1.)
3-5
3 Place the T-P2 polarizer into the optical path and
make a crossed Nicol’s position. (See 3.23.8.)
3-1
4 To perform the sensitive color polarization
microscopy, place the TI-DIC λplate into the
optical path. (See 3.23.8.)
When using the sextuple revolving nosepiece, use
the D-LP λplate instead of the TI-DIC λplate, if
desired. (See 3.18.1.)
3-2
5 Turn the light intensity control dial on the TI-PS
100W power supply to adjust the brightness of the
diascopic illumination.
6 Push in or pull out the desired ND field for the
supporting pillar for dia-Illuminator to place it into
the optical path, then adjust the brightness for the
view-field. (See 3.23.5.)
Return to the bright-field microscopy under the diascopic illumination.
1 Remove the analyzer from the optical path.
2 Remove both the T-P2 polarizer and the λplate
from the optical path.
3 Turn the light intensity control dial on the TI-PS
100W power supply to adjust the brightness of the
diascopic illumination.
4 Push in or pull out the desired ND field for the
supporting pillar for dia-Illuminator to place it into
the optical path, then adjust the brightness for the
view-field.
28
3
3.1
3.1.1
Operation Details
Power ON/OFF
Power of the microscope
The power switch for the product is located beside
the AC inlet on the rear of the microscope. To turn on
the product, set the power switch to the “|” side. To
turn off the microscope, set the power switch to the
“c” side.
The power LED on the front lights up when the power
is turned on.
Power switch
“|” side: ON
“c” side: OFF
POWER LED lights up
Figure 3.1-1 Power of the microscope
■ Turning on the microscope during the use of an external light source
To use the HG precentered fiber illuminator or the supporting pillar for dia-Illuminator 100W provided as an
external light source, set the power switch on the external light source to ON in advance, then set the power
switch on the MA200 main body to ON. (See 3.22.1 and 3.23.1.)
3.1.2
Power supply of the lamp
The product has a built-in power supply circuit for the halogen lamp. When the specified lamphouse
(LV-LH50PC) is attached, the lamp is lit in connection with the power supply for the microscope.
29
Chapter 3
3.2
3.2.1
Operation Details
Illumination
Brightness control and illumination ON/OFF
Adjust the brightness of the lamp for the lamphouse
(LV-LH50PC) with the brightness control dial on the
left of the microscope. With the power switch
remained on, turn the dial clockwise to increase the
brightness or turn the dial counter-clockwise to
decrease it. Turn the dial to OFF to turn off the lamp.
Brightness range
OFF
position
Index
Brightness control dial
Figure 3.2-1 Brightness control and illumination
ON/OFF
3.2.2
Switching the Internal/External brightness control
To adjust the lamp for the lamphouse (LV-LH50PC)
with the brightness control dial, be sure to set the
Internal/External brightness control changeover
switch [EXTERNAL ON/OFF] to OFF (internal mode)
on the rear. To adjust the brightness of the
lamphouse on the PC, set the changeover switch to
ON (external mode).
Internal/external brightness control
changeover switch
OFF: adjusts the brightness on the
microscope
Figure 3.2-2 Switching the Internal/External
brightness control
30
Chapter 3
3.2.3
Operation Details
Displaying the POWER LED
The status of the illumination lamp appears on the
Power LED located on the front of the microscope.
POWER switch OFF: LED lights off
POWER switch ON, brightness control dial OFF:
orange lights up
POWER switch ON, brightness control dial ON:
green lights up
The display status remains the same when an
external PC is in use.
POWER LED
LED lights off: POWER switch OFF
Orange LED lights up: POWER switch ON; brightness control dial OFF
Green LED lights up: POWER switch ON; brightness control dial ON
Figure 3.2-3 Displaying the POWER LED
■ Color temperature of illuminator
Adjusting brightness with the brightness control dial will affect the lamp color temperature and alter the color
balance of the image.
When accurate color reproduction is critical to observe the color balance of the sample or to shoot/record its
image, set the brightness control dial to the index as shown (at the 3 O’clock position of the dial in the Figure
3.2-1) and place the NCB11 filter into the optical path. In this condition, the voltage applied to the lamp is
approximately 9 V and the color reproduction is improved maximally. To adjust the brightness of the
illumination, use ND filters. (See 3.10.)
3.3
Selecting the Microscopy Method
The setting options for the BD field changeover lever or the settings for the optional items required for this
microscope is shown in Table 3.3-1.
BD field changeover lever
Press (BF): bright-field
Pull (DF): dark-field
Figure 3.3-1 Setting options for the BD field changeover lever
31
Chapter 3
Table 3.3-1
Microscopy
Revolving
nosepiece
Objective
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
BF
microscopy
under the
epi
illumination
5 revo.,
6 revo.,
7 revo.
DF
microscopy
under the
epi
illumination
5 revo. only BD objective only
Operation Details
Selecting the microscopy method
Items required for the optical BD changeover
path
lever
Remarks
BF (Press)
Normal bright-field microscopy under the
episcopic illumination.
DF (Pull)
Pull out BD field changeover lever to perform
dark-field microscopy under the episcopic
illumination.
⎯
• For 6 or 7 revo., BD
objective unavailable
Pull out BD field changeover lever to fully
auto-open field/aperture diaphragm (The dial
position remains the same).
⎯
Pull out BD field changeover lever to exclude
polarizer/analyzer unit or fluorescent unit when
it is in the optical path.
Simplified
polarization
microscopy
under the
epi
illumination
5 revo.,
6 revo.,
7 revo.
DIC
microscopy
under the
epi
illumination
5 revo.,
6 revo.
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• For 6 or 7 revo., BD
objective unavailable
• MA2-PA/MA2-UPA
polarizer/analyzer unit
BF (Press)
• For the sensitive color
polarization microscopy,
MA2-λP λ plate available
LU EPI objectives or LU For 5 revo.:
BD objective only
• MA2-PA polarizer/analyzer
• For EPI objectives
unit
with 5 revo., LU
• L-DIHC/L-DIC slider (single
nosepiece adapter
NR method)
M32-25 required
• For the sensitive color
• BD objective
polarization microscopy,
unavailable for 6
MA2-λP λ plate available
revo.
For 6 revo.:
Set bright-field microscopy under the episcopic
illumination. And then, attach
polarizer/analyzer unit to make crossed Nicol’s
position.
Push λ plate into polarizer/analyzer unit and
place it into optical path.
BF (Press)
Set bright-field microscopy under the episcopic
illumination. And then, attach
polarizer/analyzer unit to make crossed Nicol’s
position. Place DIC prism into optical path to
perform DIC microscopy.
To adjust the contrast, turn the prism setting
dial for the single NR method, whereas turn the
polarizer rotation ring for Senarmont method.
Push λ plate into polarizer/analyzer unit and
place it into optical path.
• MA2-UPA polarizer/analyzer
unit
• LV-DIHC/LV-DIC slider
(Senarmont method)
• For the sensitive color
polarization microscopy,
MA2-λP λ plate available
Epi-fl
microscopy
5 revo.,
6 revo.,
7 revo.
LU EPI objectives or LU • MA2-FL fluorescent unit
BD objective only
BF (Press)
Place a desired fluorescent unit G, B, BV or V
into optical path to perform epi-fl microscopy.
BF (Press)
Normal bright-field microscopy under the
diascopic illumination.
BF (Press)
Set bright-field microscopy under diascopic
illumination. And then, attach polarizer to
diascopic pillar illuminator and insert analyzer
into MA200 main body to make crossed Nicol’s
position. (For polarizer/analyzer unit, the
analyzer part is used.)
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• For 6 or 7 revo., BD
objective unavailable
BF
microscopy
under the
dia
illumination
5 revo.,
6 revo.,
7 revo.
Simplified
polarization
microscopy
under the
dia
illumination
5 revo.,
6 revo.,
7 revo.
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
⎯
• For 6 or 7 revo., BD
objective unavailable
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• For 6 or 7 revo., BD
objective unavailable
• T-P2 polarizer
• MA2-PA/MA2-UPA
polarizer/analyzer unit (for 6
revo., D-DA analyzer is also
available)
• For the sensitive color
polarization microscopy,
TI-DIC λ plate available (for 6
revo., D-LP λ plate is also
available)
32
Attach TI-DIC λ plate to supporting pillar for
dia-Illuminator 100W. For 6 revo., D-LP λ plate
is also available.
Chapter 3
3.4
3.4.1
Operation Details
Eyepiece Tube
Selecting optical path
• The light distribution for both the binocular and vertical tube parts of the MA2-TI3 trinocular eyepiece tube
can be performed using the optical path changeover lever.
Light distribution
Lever
position
Binocular tube
Vertical tube
IN (Press)
100
0
OUT (Pull)
0
100
Optical path
changeover lever
Figure 3.4-1 Selecting optical path
• The light distribution for the eyepiece tube part and the back port can be selected with the eyepiece
tube/back port changeover lever.
Eyepiece tube
Back port
IN (Press)
100
0
OUT (Pull)
55
45
Eyepiece tube/back port
changeover lever
Figure 3.4-2 Selecting optical path
33
Light distribution
Lever
position
Chapter 3
3.4.2
Operation Details
Adjusting the eyelevel risers
Eyelevel risers can be used to adjust the height of the
eyepiece tube for the user’s eye point. Up to two
eyelevel risers can be attached in piles. When one
eyelevel riser is attached, the eyepiece height rises
25 mm.
Eyelevel riser
If you look through the objective that is 10X or
less than 10X when two eyelevel risers are
attached on the microscope, the rim of the
view-filed may look different from the one without
an eyelevel riser due to reduction of light
intensity.
Figure 3.4-3 Adjusting the eyelevel risers
3.5
Adjusting the Interpupillary Distance
Adjust the interpupillary distance or the distance between the eyepieces according to user’s eye distance.
Before adjusting the interpupillary distance, follow the
procedure described in “2.1 Bright-field Microscopy
under the Episcopic Illumination” or “2.6 Bright-field
Microscopy under the Diascopic Illumination,” and
focus on the sample with the 10X objective.
Look through the eyepieces and adjust the binocular
part until the view fields for the right and left eyes
coincide.
This will facilitate the observation with both eyes.
Figure 3.5-1 Adjusting the interpupillary distance
The binocular part has a scale for interpupillary
distance. It is recommended to memorize or record
your interpupillary distance, so that the distance
between the eyepieces can be readily adjusted next
time.
34
Chapter 3
3.6
Operation Details
Adjusting the Diopters
Adjust the diopter according to the user's eyesight using the diopter adjustment rings on the eyepieces.
Diopter adjustment corrects the difference in the left and right eyesight. This adjustment facilitates binocular
observation and minimizes focal deviation when switching objectives. Make sure to adjust the diopter
adjustment rings on both eyepieces.
Diopter
adjustment ring
Outer tube edge
Engraved line
Positioning
groove
Diopter adjustment reference position
1 Turn the diopter adjustment rings of the eyepieces
and align their engraved lines with the edges of
the outer tubes of the eyepieces. This indicates
the reference positions for the diopter rings.
2 Follow the procedure described in “2.1 Bright-field
Microscopy under the Episcopic Illumination” or
“2.6 Bright-field Microscopy under the Diascopic
Illumination,” and focus the sample with the 10X
objective.
3 Place the 50X objective into the optical path. Turn
the coarse/fine focus knobs to focus on the
sample.
4 Place the 5X or 10X objective into the optical path.
5 Look into the right eyepiece with your right eye,
not touching the coarse/fine focus knob, and focus
on the sample by turning the diopter adjustment
ring on the right eyepiece.
6 Look into the left eyepiece with your left eye, not
touching the coarse/fine focus knob, and focus on
the sample by turning the diopter adjustment ring
on the left eyepiece.
Figure 3.6-1 Adjusting the diopters
7 Repeat the steps from (3) to (6) until the image
stays in focus even when the objective is changed.
35
Chapter 3
3.7
3.7.1
Operation Details
Adjusting the Focus (for focus operation)
Using the coarse/fine focus knob
Focus on the sample lifting/lowering the revolving
nosepiece with the fine/coarse focus knob.
The relationship between the direction of coarse/fine
focus knob rotation and the revolving nosepiece
vertical movement is shown in the figure. To raise the
revolving nosepiece, turn the knob forward.
• One revolution of the coarse focus knob drives the
revolving nosepiece approximately 4.0 mm.
• One revolution of the fine focus knob drives the
revolving nosepiece approximately 0.1 mm.
• The fine focus knob is marked in 1 μm.
• The coarse/fine focus stroke (the vertical movable
range of the revolving nosepiece) is 4 mm. (±2
mm: top datum surface for stage)
To raise the revolving nosepiece, turn the knob forward.
Figure 3.7-1 Relationship between focus knob
rotation and revolving nosepiece vertical movement
Do not attempt following operations, because
doing so may cause the product failure.
• Turning the left and right knobs in opposite
directions at the same time.
• Keep turning the coarse/fine knobs after hitting
the limits.
3.7.2
Adjusting the torque for the coarse focus ring
The rotational stiffness of the coarse focus knob can
be adjusted as follows.
To make it stiffer, turn the coarse torque adjustment
ring behind the coarse focus knob in the direction of
the arrow indicated in the figure.
Stiffer
To make it looser, turn the knob in the opposite
direction.
Coarse torque adjustment ring
Figure 3.7-2 Adjusting the torque
for the coarse focus ring
36
Chapter 3
3.8
3.8.1
Operation Details
Placing the Sample and Operating the Stage
Placing the sample
Sample holder
Sample
Sample clip
Normally, a sample can be placed on the stage with
the sample holder. It can be also placed directly on
the stage without the sample holder. In either case,
be sure that the observation position comes to the
center of the view-field.
Figure 3.8-1 Placing the sample
■ Sample holder
To use the sample holder, place it onto the stage.
The MA2-SR rectangular stage is supplied with the sample holder (outer diameter: φ108 mm, thickness: 2 mm,
teardrop-shaped aperture: φ22 mm). This sample holder is also supplied with the sample clip to hold the
sample set on the stage. Use the clip to prevent the sample from tipping over or being moved.
The sample holder is supplied with a rotation knob. If you turn the ring holding the rotation knob, you can turn
the sample with the observation position unmoved.
Various options are available for the sample holder as shown below. Select an appropriate option according
to the size or observation method of the sample used.
Item
Model
Remarks
Sample holder
Standard Holder
(attached to stage)
Outer diameter φ108 mm; thickness: 2 mm; teardrop-shaped
aperture: φ22 mm; supplied with the rotation knob and a sample
clip
MA-SRSH 40
φ40 mm, supplied with the rotation knob and a sample clip
MA-SRSH 10
φ10 mm, supplied with the rotation knob and a sample clip
MA-SRSH 25-40
φ25×40 mm, supplied with the rotation knob and a sample clip
MA2-SRSH
Holder set (φ23, 30, 36 mm) supplied with the rotation knob and
a sample clip
MA-SRSH1
φ15 mm
37
Chapter 3
3.8.2
Operation Details
Changing the Observation Position
The rectangular stage is equipped with the knob to
move the stage in all four directions.
The upper knob is used for Y direction (back and
forth), and the lower knob for X direction (left and
right).
The stage knob has a universal joint that allows you
to move the stage knob direction being tilted at 28
degrees. This offers easy operation and user
convenience for switching between the focus knob
and the stage knob.
The stage is equipped with the graduations that move
50 mm (max.) to X or Y direction.
Figure 3.8-2 Changing the observation position
• A cross roller guide is provided to move the
stage in the X/Y direction. The Y direction
movement rack is located at the bottom of the
stage. Do not touch the rack during your
operation, as you may get injured.
• If you move the stage plate directly by hands,
the stage will be damaged. Make sure to use
these stage knobs to move the stage.
38
Chapter 3
3.9
3.9.1
Operation Details
Operating the Revolving Nosepiece and the Objective
Revolving nosepiece in combination with the objective
For the MA200, you can use various revolving nosepieces including the quintuple, sextuple or septuple
revolving nosepiece to attach the industrial CFI objective lens. The combination varies depending on the
microscopy applied. Refer to “4.2 Combination List for the Unit” to select an appropriate combination.
■ LU nosepiece adapter M32-25
The adapter runs to switch the screw diameter from large to small for the objective hole on the revolving
nosepiece. To attach EPI objectives on the quintuple revolving nosepiece, screw the LU nosepiece adapter
M32-25 into the hole on the revolving nosepiece.
3.9.2
Changing the objectives
Turn the revolving nosepiece to the click-stop position
by hand to switch the magnification for objectives.
The observing area may be out of the optical path
hole if the observation position is adjusted by
moving the stage. Do not turn the revolving
nosepiece under such condition, or the objective
may touch the stage. When turning the revolving
nosepiece, be sure that the objective does not
touch the sample or the stage.
Turn the revolving nosepiece to the click-stop position
Figure 3.9-1 Changing the objectives
3.9.3
Normally, attach the objectives so that the
magnification increases as the nosepiece is turned
clockwise as viewed from above.
Displaying the address for the revolving nosepiece
The nosepiece addresses (1 to 7) on the front-display
light up to indicate the position of the revolving
nosepiece.
The address for the revolving nosepiece
placed in the optical path lights up
Figure 3.9-2 Displaying the address for the
revolving nosepiece
39
Chapter 3
3.10
Operation Details
Filter
Two quartet filter turrets are provided on the
microscope, and each filter turret has the φ25 mm
filters as shown below.
Filter currently
in the optical
path displayed
Filter turret 1
Each time you turn the turret 90 degrees, the next
filter enters the optical path. The display indicated on
the turret is the filter placed in the optical path. Select
the filter suitable for your purpose, and place it into
the optical path by turning the turret.
Each turret is provided with an optional (USER) slot
to add a desired filter. Use the slot if necessary. (For
attaching an optional filter, refer to “4.11.”)
Filter turret 2
Figure 3.10-1
Filter turret
Turret position
Turret 1:
on user-side
Turret 2:
on far-side
Filter
Indication
Usage
ND16
(Neutral Density filter)
ND16
Adjusts the brightness for general microscopy
or photomicroscopy. Reduces the light intensity
to 1/16. (Transmittance: approx. 6.3%)
ND4
(Neutral Density filter)
ND4
Adjusts the brightness for general microscopy
or photomicroscopy. Reduces the light intensity
to 1/4. (Transmittance: approx. 25%)
Optional
USER
Attach as desired
Dummy
OPEN
Without filters
GIF (Green Interference
filter)
GIF
Improves the contrast for microscopy under a
monochrome light or when performing
monochrome imaging.
NCB11 (Neutral Color
Balancing filter)
NCB
Corrects the color temperature for general
microscopy or filming by daylight color TV
camera photography. Color reproduction is best
when this filter is inserted into the optical path,
and the lamp voltage is set to the same display
as the lamp rating. Remove from optical path
when performing monochrome imaging.
Optional
USER
Attach as desired
Dummy
OPEN
Without filters
40
Chapter 3
3.11
Operation Details
Adjusting the Field Diaphragm (for the episcopic Illumination)
Image of the field
diaphragm
The field diaphragm is used to limit the irradiation
area of the lamp to the view-field of the microscope.
The field diaphragm dial (F. S.) changes the opening
of the field diaphragm. Normally, stop down the field
diaphragm to a size that circumscribes (or inscribes)
the view-field. An unnecessarily large irradiation area
will result in stray light and flare, thereby reducing the
contrast of the optical image. For photomicroscopy,
the field diaphragm should be stopped down to a size
slightly larger than the area to be captured. Avoid
stopping down the field diaphragm too close to the
frame size to be captured, as it may result in
vignetting.
View-field of the
eyepiece
*
Field diaphragm knob
Figure 3.11-1
The adjustment of the field diaphragm opening
should be performed after centering the
diaphragm.
Field diaphragm
centering holes
Field Diaphragm Adjustment
■ Centering procedure for the field diaphragm
Image of the field diaphragm
1 Follow the procedure described in “2.1 Bright-field
Microscopy under the Episcopic Illumination,” and
focus on the sample with the 10X objective.
2 Stop down the field diaphragm by turning the field
diaphragm dial.
View-field of the eyepiece
Figure 3.11-2
Centering the field diaphragm
3 Insert hexagonal screwdrivers into the field
diaphragm centering holes and turn the internal
adjustment screws to bring the field diaphragm
image to the center of the view-field.
4 Adjust the field diaphragm image with the field
diaphragm dial and centering screws so that it
inscribes the view-field.
5 To observe the sample, turn the field diaphragm
dial so that the field diaphragm image
circumscribes the view-field.
41
Chapter 3
3.12
Operation Details
Adjusting the Aperture Diaphragm (for the episcopic illumination)
Objective pupil
Aperture
diaphragm
The aperture diaphragm controls the numerical
aperture of the illumination system, and is closely
related to the resolution of the optical image, the
brightness, the contrast and the depth of focus.
When the size of the aperture diaphragm is stopped
down, the resolution and the brightness are reduced
while the contrast and the depth of focus are
increased. However, when the size of the aperture
diaphragm is increased, the resolution and the
brightness are increased while the contrast and the
depth of focus are reduced.
The resolution, the brightness, the contrast and the
depth of focus are related to one another and cannot
be changed independently. Set the conditions to fit
the requirements of the sample and the intended
application.
Aperture
diaphragm dial
Figure 3.12-1
Aperture diaphragm
centering holes
Aperture diaphragm adjustment
The aperture diaphragm is adjusted by operating the
aperture diaphragm dial (A.S.) on the front of the
microscope. Adjust the aperture diaphragm properly
according to the procedure below. Normally, the
aperture diaphragm should be adjusted to about 70 to
80% of the numerical aperture of the objective.
When the reflectance of the sample is low, the
diaphragm image may not be seen. In this case,
change to a sample of a near-polished surface.
■ Centering procedure for the aperture diaphragm
Image of aperture diaphragm
Objective pupil
Figure 3.12-2
Centering the aperture diaphragm
1 Follow the procedure described in “2.1 Bright-field
Microscopy under the Episcopic Illumination,” and
focus on the sample with the 10X objective.
2 Remove one eyepiece to look into the eyepiece
tube to check that the aperture diaphragm image
is seen in the objective pupil in the eyepiece tube.
3 Stop down the aperture diaphragm by turning the
aperture diaphragm dial.
4 Insert hexagonal screwdrivers into the aperture
diaphragm centering holes and turn the internal
adjustment screws to bring the aperture
diaphragm image to the pupil center of the
objective.
5 Operate the aperture diaphragm dial and the
centering screws so that the aperture diaphragm
image inscribes the objective pupil.
6 When starting observation, adjust the aperture
diaphragm dial so that the aperture diaphragm
image becomes 70 to 80% of the numerical
aperture of the objective. (Perform this adjustment
for each objective.)
42
Chapter 3
Operation Details
■ Adjustment with centering telescope (C-CT)
Remove one of the eyepieces and attach the centering telescope instead. Turn the eyepiece of the centering
telescope to adjust the focus. This will allow you to view the objective pupil (a bright circle) and the aperture
diaphragm image.
3.13
Using the Polarizer/Analyzer Unit (MA2-PA/MA2-UPA)
Second click-stop position
First click-stop position
Polarizer rotation ring
Polarizer
Analyzer
Slot for λ plate
Figure 3.13-1
Polarizer/analyzer unit
Attach the polarizer/analyzer unit to the MA200 main
body, then use it for the simplified polarization
microscopy under the episcopic/diascopic
illumination, and the differential interference contrast
microscopy under the episcopic illumination.
MA2-PA or MA2-UPA is equipped with both the
polarizer and the analyzer with the slot provided to
insert the λ plate for the sensitive color microscopy
(see 3.14). A single push or pull of the unit allows you
to insert or remove both the polarizer and analyzer
from the optical path at the same time.
The MA2-UPA is equipped with the 1/4 λ plate. If
using the MA2-UPA for polarization observation, the
low-contrast sample such as aluminum can be
observed with clear contrast.
For the simplified polarization microscopy under the
diascopic illumination, only analyzer of the unit is
used.
3.13.1
Inserting/removing the polarizer/analyzer from the optical path
Polarizer/analyzer unit
Push the unit attached into the microscope, and once
the polarizer/analyzer hits the second click-stop
position the polarizer/analyzer enters the optical path.
Pull out the unit in advance, and when it hits the first
click-stop position the polarizer/analyzer is removed
from the optical path.
Figure 3.13-2 Inserting/removing the
polarizer/analyzer from the optical path
43
Chapter 3
3.13.2
Operation Details
Adjusting the polarizer direction
Each time you turn the polarizer rotation ring, the
direction of the polarizer is changed. You can turn the
polarizer 360 degrees.
When the “+” mark on the polarizer rotation ring is
aligned with the “T” mark, the crossed Nicol’s
position is made. When the “=” mark on the rotation
ring is aligned with the “T” mark, the open Nicol’s
position is made.
(1)
(2)
Polarizer direction
Open Nicol’s
position (2)
Remove one eyepiece from the eyepiece tube and
look inside the open sleeve. You can see the
objective pupil as a bright circle.
Turn the polarizer rotation ring in either direction until
a dark cross appears in the view-field as shown in the
figure. This is the crossed Nicol’s position.
Dark cross
Figure 3.13-3
Checking the crossed Nicol’s position
Place the unit in the optical path. And then, place a
sample with a flat and plain surface onto the stage.
Set up the microscope for the simplified polarization
microscopy under the episcopic illumination.
(1)
Crossed Nicol’s
position (1)
■
Adjusting the polarizer direction
44
Chapter 3
3.14
Operation Details
Using the λ Plate (MA2-λP)
First click-stop
position
Attach the λ plate to the polarizer/analyzer unit to
perform sensitive color polarization microscopy.
The λ plate cannot be used for the simplified
polarization microscopy under the diascopic
illumination.
Second click-stop position
λ plate
Figure 3.14-1
3.14.1
λ plate slider
Inserting/removing the λ plate
Push the λ plate slider into the polarizer/analyzer unit
placed in the optical path. When it hits the second
click-stop position, the λ plate enters the optical path.
Pull out the λ plate slider, and the λ plate is removed
from the optical path when it hits the first click-stop
position.
λ plate slider
Figure 3.14-2
Inserting/removing the λ plate
45
Chapter 3
3.15
Operation Details
Using the DIC Slider (L-DIHC/L-DIC)
Prism position index
Prism position
Sliding limit groove
Prism setting dial
Prism movement
knob
Figure 3.15-1
3.15.1
First
click-stop
position
Attach the L-DIHC/L-DIC DIC slider to the quintuple
nosepiece (MA2-NUI5 or LV-NU5A) in combination
with the MA2-PA polarizer/analyzer unit to perform
the DIC microscopy under the episcopic illumination
in single NR method.
The L-DIHC slider is designed for high contrast
observation.
Second click-stop
position
L-DIHC/L-DIC slider
Inserting/removing the DIC prism from the optical path
DIC slider
Push the slider attached to the revolving nosepiece.
When it hits the second click-stop position, the DIC
prism enters the optical path.
Pull out the DIC slider. When it hits the first click-stop
position, the DIC prism is removed from the optical
path.
Figure 3.15-2
Inserting/removing the DIC prism
from the optical path
3.15.2
Setting the DIC prism
For the DIC microscopy, switch the DIC prism
depending on the objective to be used.
Figure 3.15-3
DIC prism position indication
The industrial LU objective has a character (A or B)
showing the DIC prism position, which is indicated
next to the magnification/N.A. value on the objective
barrel. The figure shows the character “A” indicated
on the objective. Turn the prism setting dial to align
the index with the “A”.
46
Chapter 3
3.15.3
Operation Details
Interference color
You can change the interference color continuously by turning the prism movement knob.
Interference color
Dark color
Observations similar to the dark-field microscopy can be performed.
Gray
Phase contrast distribution of the whole sample with a bird’s eye view can be
observed.
Sensitive red-violet
3.16
Characteristics
Observations with the highest color contrast can be performed.
Using the DIC Slider (LV-DIHC/LV-DIC)
DIC Slider
Figure 3.16-1
3.16.1
The LV-DIHC/LV-DIC DIC slider is attached to
sextuple nosepiece revolver (D-ND6) in combination
with the MA2-UPA polarizer/analyzer unit for DIC
microscopy under the episcopic microscopy in
Senarmont method. The LC-DIHC is designed for
high contrast observation.
LV-DIHC/LV-DIC Slider
Selecting the DIC slider
Both the LV-DIHC and the LV-DIC sliders have A and B types to be selected, according to the objective.
The industrial LU objective has a character (A or B) showing the DIC prism position, which is indicated next to
the magnification/N.A. value on the objective barrel (refer to Figure 3.15-3). Select the DIC slider as indicated
on the objective. In Figure 3.15-3, the character “A” is indicated next to the magnification/ N.A. value. Use the
A-type DIC slider as indicated.
3.16.2
Inserting/removing the DIC slider from the optical path
Push the slider into the revolving nosepiece to the limit and the DIC prism enters the optical path.
To remove the DIC prism from the optical path, remove the slider from the revolving nosepiece.
47
Chapter 3
3.16.3
Operation Details
Interference color
You can change the interference color continuously by adjusting the orientation of the polarizer. (Refer to
3.12.3.)
Interference color
Dark color
Observations similar to the dark-field microscopy can be performed.
Gray
Phase contrast distribution of the whole sample with a bird’s eye view can be
observed.
Sensitive red-violet
3.17
Characteristics
Observations with the highest color contrast can be performed.
Using the Analyzer Slider (D-DA)
Attach the D-DA analyzer slider to the sextuple
revolving nosepiece (D-ND6) to perform the simplified
polarization microscopy under the diascopic
illumination.
Analyzer
First click-stop
position
Second click-stop
position
Figure 3.17-1
3.17.1
The D-DA analyzer is not required when the
polarizer/analyzer unit (MA2-PA/MA-UPA) is attached
to the microscope during the simplified polarization
microscopy under the diascopic illumination.
Analyzer slider
Inserting/removing the analyzer from the optical path
Two slots are provided on the front of the revolving
nosepiece. Insert the D-DA analyzer slider with the
nameplate facing down into the slot (with “A”
indicated) far from the objective. When it hits the
second click-stop position, the analyzer enters the
optical path.
Pull out the D-DA analyzer slider. When it hits the first
click-stop position, the analyzer is removed from the
optical path to enter the dummy hole.
The analyzer direction is set to the backward or front
of the microscope.
Analyzer slider
Figure 3.17-2 Inserting/removing the analyzer
from the optical path
48
Chapter 3
3.18
Operation Details
Using the λ Plate (D-LP)
λ plate
First click-stop
position
Attach the D-LP λ plate to the sextuple revolving
nosepiece (D-ND6) to perform sensitive color
polarization microscopy under the diascopic
illumination.
The D-LP λ plate is not required to use the TI-DIC λ
plate on the supporting pillar for dia-Illuminator 100W.
Second click-stop
position
Figure 3.18-1
3.18.1
λ plate slider
Inserting/removing the λ plate from the optical path
Two slots are provided on the front of the revolving
nosepiece. Insert the D-LP analyzer slider with the
nameplate facing down into the slot (with “λ”
indicated) near the objective. When it hits the second
click-stop position, the λ plate enters the optical path.
Pull out the slider, and when it hits the first click-stop
position the first red compensator is removed from
the optical path. The dummy hole then enters the
optical path.
λ plate slider
Figure 3.18-2 Inserting/removing the λ plate
from the optical path
49
Chapter 3
3.19
Operation Details
Using the Fluorescent Unit (MA2-FL)
Attach the fluorescent unit to the MA200 main body to
perform the epi-fl microscopy.
Second click-stop position
First click-stop position
A fluorescent unit consists of two types of optical
components: an excitation light filter (EX filter), a
barrier filter (BA filter). You can use the four types of
combination, "G", "B", "BV" or "V" as shown in the
table below. Select the fluorescent unit suitable for
your purpose and sample.
Compensation filter
attachment
Excitation
light filter
Barrier filter
Figure 3.19-1
To perform epi-fl microscopy using the
C-HGFI/C-HGFIE HG Precentered Fiber
Illuminator as the external light source, screw the
compensation filter supplied with the fiber
adapter into the filter attachment of the
fluorescent unit. (Refer to 4.15.1.)
Fluorescent unit
Fluorescent unit
Characteristics of EX filter (nm)
Characteristics of BA filter (nm)
MA2-FL G
510-560
590
MA2-FL B
450-490
520
MA2-FL BV
400-440
470
MA2-FL V
380-420
450
3.19.1
Inserting/removing the fl filter from the optical path
Fluorescent unit
Push in the unit attached on the microscope. When it
hits the second click-stop position, the fl filter enters
the optical path.
Pull out the unit. When it hits the first click-stop
position, the fl filter is removed from the optical path.
Under this condition, the compensation filter attached
to the fluorescent unit enters the optical path to
reduce glare in the bright-field observation. (Refer to
4.15.1.)
Figure 3.19-2 Inserting/removing the fluorescent
unit from the optical path
50
Chapter 3
3.19.2
Operation Details
Excitation light filter (EX filter)
Spectral
transmittance
EX filter
0
Bandwidth
Wavelength
An excitation light filter transmits lights selectively and
blocks other lights. The transmitted lights are called
excitation lights. They are used to excite the
fluorophore in the sample and fluorescent lights are
emitted from the sample. The wavelength range of
lights that can pass through the filter is called the
bandwidth.
The bandwidth of the excitation light filter determines
the brightness of the fluorescent image, the
occurrence of autofluorescence (fluorescence
resulting from substances other than the
fluorophores), and degree of fading. When the filter
has a wide bandwidth, a large amount of excitation
lights will be irradiated on the sample. In this case,
the image becomes bright but the amount of
autofluorescence becomes large and fading of the
sample occurs soon. On the contrary, when the filter
has a narrow bandwidth, a small amount of excitation
lights will be irradiated on the sample. In this case,
the image becomes dark but the amount of
autofluorescence becomes small and fading of the
sample occurs late. For samples with pronounced
autofluorescence, use an excitation light filter with a
narrow bandwidth. (The resulting fluorescent image
will be darker, however.)
The excitation light filter is exposed to strong lights.
Therefore it may deteriorate under use. Please
replace it at a proper interval based on the hours
used.
Narrow
Bandwidth of excitation filter
Wide
Brightness of
fluorescence image
Dark
Bright
Occurrence of
autofluorescence
Less frequent
Frequent
Degree of fading
Small
Large
51
Chapter 3
3.19.3
Operation Details
Barrier filter (BA filter)
A barrier filter transmits only fluorescent lights emitted by the sample but blocks the excitation lights. This filter
makes it possible to observe the fluorescent image without unnecessary light (that is, on a dark background).
There are two types of barrier filters: LP filters (long-pass filters), which block all lights that are shorter than a
certain boundary wavelength and allow all lights to pass that are longer than the boundary wavelength, and
BP filters (band-pass filters), which allow only lights in a certain bandwidth to pass. The LP filter is a barrier
filter that is provided on the MA200.
The boundary to block or transmit lights using an LP filter is called the cut-on wavelength.
An excitation light is a light that is irradiated to the sample. The fluorophore in the sample absorbs the
excitation light energy. As a result, fluorescent lights are emitted from the fluorophore instead. When a sample
is labeled with a fluorophore that emits fluorescent lights of very close wavelengths to the excitation light,
select a barrier filter with the shortest cut-on wavelength permitted by performance requirements for efficient
fluorescent microscopy.
A longer cut-on wavelength tends to result in a more complete separation between an excitation light and
fluorescent lights, rendering a darker background of the fluorescent image. With the recent advancement in
filter performance, however, filters with shorter cut-on wavelengths can be used for this purpose and they are
used more often than before.
■ Light source for the epi-fl microscopy
To perform the epi-fl microscopy, the standard light source (a halogen lamp) may not be able to provide the
sufficient brightness. In such case, attach an HG Precentered Fiber Illuminator (C-HGFI/ C-HGFIE) that uses
a mercury lamp as a light source to perform the episcopic illumination (refer to 3.22).
52
Chapter 3
3.20
Operation Details
Using the Scale Slider (MA2-GR/MA2-MR)
Attach the MA2-GR/MA2-MR slider to the MA200 main body to measure steel structures or to photograph
images.
3.20.1
Grain scale slider (MA2-GR)
The following two types of scales are provided for a single slider for Austenitic steel measurement.
• Austenitic steel measurement scale (ASTM E112-63 reticle numbers 1 to 8)
Compare the reticle pattern (numbers 1 to 8) with the sample-structure size in order to measure the grain
size. Normally, the 10X objective is used in measuring the grain size. To use the 20X objective, select the
reticle number two scales up, whereas to use the 5X objective, select the reticle number two scales down.
• Lattice scale (0.5 mm intervals; Vertical/Horizontal: 20 each)
The lattice scale is normally used for the Nonmetallic Inclusion test, and it can also be used for simple
lattice-measurement. The lattice, whose intervals on a reticle indicate 0.5 mm, consists of 20 vertical or
horizontal lines. When the 10X objective is used, the lattice intervals equivalent to those on the sample
become 0.05 mm.
Austenitic steel
measurement scale
When inserted, the grain scale slider is pressed down
by a leaf spring installed inside of the slot to adjust
the desired inserting position. To place the scale in
the optical path, use the icons to indicate the current
scale type on the slider and the positioning index line.
Lattice scale
Positioning index line
Figure 3.20-1
Grain scale slider
53
Chapter 3
3.20.2
Operation Details
Scale slider (MA2-MR)
Three items of reticles including “magnification,”
“length indication” by a line, and length indication by
the unit of “xx μm” are provided for photographic
images.
The reticle is compatible with 5X, 10X, 20X, 40X, 50X
and 100X objectives, and turn the dial of the slider to
switch to the reticle which suits the magnification of
the objective in the optical path. To increase the scale
magnification, turn the dial as the arrow in the left
figure shows.
Reticle
Figure 3.20-2
When inserted, the scale slider is pressed down by a
leaf spring built inside the slot to adjust the desired
inserting position. Adjust its position to locate the
reticle on the right hand side of the view-field.
Scale slider
*
When inserting the slider into the slot, face its side with the indication toward the front of the microscope.
*
To capture the scale using the camera, adjust the desired inserting position by checking the actual scale
position or area on the DS-L2 monitor or a PC monitor.
*
Reticle patterns may not be clearly visible on a dark background as in the case of dark-field microscopy.
*
Do not touch or press hard against the glass. It may cause damage to the scale reticle.
*
Remove the dust on the glass with a soft blower or brush.
54
Chapter 3
3.21
Operation Details
Using the Intermediate Magnification Unit (MA2-MC)
Attach the intermediate magnification unit to the
MA200 main body to change the observation
magnification for the binocular, vertical tube or the
back port.
Turn the turret for this unit to select the desired
magnification from the magnifications 1X (dummy),
1.5X and 2X. Set the turret at the desired
magnification displayed on the turret (click-stop).
Magnification
Magnification changeover turret
Figure 3.21-1
Intermediate magnification unit
55
Chapter 3
3.22
Operation Details
Using the HG Precentered Fiber Illuminator (C-HGFI/C-HGFIE)
The specified light source LV-LH50PC may not
provide sufficient brightness to perform epi-fl
microscopy. In such case, use an HG precentered
fiber illuminator that uses a high intensity mercury
lamp as a light source.
HG Precentered
Fiber Illuminator
Figure 3.22-1
HG Precentered Fiber Illuminator
3.22.1
Procedure for turning on the power switch
1 Be sure that the fiber illuminator is properly
connected to the MA200 main body.
2 Set the POWER switch to the “|” side to turn on
the fiber illuminator and wait until it starts up.
When the POWER indicator on the fiber
illuminator lights up, the LAMP indicator starts
blinking. Once the illuminator starts up, the LAMP
indicator stops blinking and lights up.
2
Power switch ON
If the C-HGFIE motorized fiber illuminator is in
use, the communication function on the illuminator
does not activate until it starts. Be sure to wait until
the illuminator starts up.
3 Set the POWER switch to the “|” side to turn on
the power on the MA200 main body.
The power LED on the front of the MA200 main
body lights up.
4 To turn off the fiber illuminator, set the power
switch to the “c” side in the sequence of the fiber
illuminator, the MA200 main body.
*
LAMP indicator
POWER indicator
1
Power switch ON
Figure 3.22-2 Procedure for turning on
the power switch
56
To use the HG precentered fiber illuminator
correctly, carefully read the instruction manual
supplied with the fiber illuminator and make sure
to follow the instruction.
Chapter 3
3.23
Operation Details
Using the supporting pillar for dia-Illuminator 100W (MA2-DP)
Attach the supporting pillar for dia-Illuminator 100W to the MA200 main body to perform the microscopy under
the diascopic illumination.
To perform the microscopy, mount the D-LH/LC Lamphouse and condenser etc. to the supporting pillar for
dia-Illuminator 100W, and connect the power supply (TI-PS 100W) to the lamphouse.
Lamphouse
Filter slider
Supporting pillar for
dia-Illuminator 100W
Condenser mount
Condenser
Power supply
Figure 3.23-1
3.23.1
Supporting pillar for dia-Illuminator 100W
Procedure for turning on the power switch
1 Be sure that the lamphouse on the supporting
pillar for dia-Illuminator is properly connected to
the power supply, and the brightness control dial
on MA200 main body is set to OFF.
2 Set the POWER switch to the “|” side on the power
supply.
The POWER indicator on the power supply and
the lamp light up.
2
Power switch ON
3 Set the POWER switch to the “|” side to turn on
the MA200 main body.
The power LED on the front lights up.
POWER indicator
4 To turn off the pillar illuminator, set the power
switch to the “c” side in the sequence of the fiber
illuminator, the MA200 main body.
*
1
Light intensity
control dial
Power switch ON
Figure 3.23-2 Procedure for turning on
the power switch
57
To adjust the brightness of the diascopic
illumination or to use the TI-PS 100W power
supply in detail, refer to the instruction manual
supplied with the equipment.
Chapter 3
3.23.2
Operation Details
Focusing and centering the condenser
To perform the diascopic illumination for the first time or to replace the current condenser, focus and center
the condenser so that the light through the condenser is focused on the correct position of the sample surface
(at the center of the optical path).
1 Be sure to focus the 10X objective on the sample
as described in “2.6 Bright-field Microscopy under
the Diascopic Illumination.”
Field
diaphragm
dial
2 Stop down the field diaphragm by turning the field
diaphragm dial on the supporting pillar for
dia-Illuminator 100W until the field diaphragm
image comes into the view-field.
Condenser
focus knob
Condenser
focus knob
Condenser
centering
screws
Field diaphragm image
View-field
Move the field diaphragm image into the center of the
view-field. Adjust its size to match the view-field.
Figure 3.23-3 Focusing and centering
the condenser
3 Turn the condenser focus knob of the supporting
pillar for dia-Illuminator to focus on the field
diaphragm image.
4 Turn the condenser centering screws (2 for both
sides) for the supporting pillar for dia-Illuminator to
move the field diaphragm image to the center of
the view-field.
5 Place the 50X objective into the optical path.
6 Adjust the size of the field diaphragm image to
closely match the size of the view-field, by turning
the field diaphragm dial on the supporting pillar for
dia-Illuminator.
7 Turn the condenser centering screws on the
supporting pillar for dia-Illuminator to move the
field diaphragm image to the center of the
view-field.
*
58
To observe the sample, turn the field diaphragm
dial so that the field diaphragm image
circumscribes the view-field. (Perform this
adjustment for each objective.)
Chapter 3
3.23.3
Operation Details
Adjusting the field diaphragm (for the diascopic illumination)
The field diaphragm is used to limit the irradiation area of the lamp to the view-field of the microscope.
View the supporting pillar for dia-Illuminator 100W from above, then turn the field diaphragm dial
counter-clockwise to open the field diaphragm, or turn the field diaphragm dial clockwise to stop down the
diaphragm. Normally, stop down the field diaphragm to a size that circumscribes (or inscribes) the view-field.
An unnecessarily large irradiation area will result in stray light and flare, thereby reducing the contrast of the
optical image. For photomicroscopy, the field diaphragm should be stopped down to a size slightly larger than
the area to be captured. Avoid stopping down the field diaphragm too close to the frame size to be captured,
as it may result in vignetting.
*
Perform the field diaphragm adjustment after completing focusing and centering for the condenser lens.
1 Place the 10X objective into the optical path.
2 Stop down the field diaphragm by turning the field
diaphragm dial on the supporting pillar for
dia-Illuminator until the field diaphragm image
comes into the view-field.
Field
diaphragm
dial
3 Turn the condenser focus knob of the supporting
pillar for dia-Illuminator to focus on the field
diaphragm image.
Condenser
focus knob
Condenser
focus knob
Condenser
centering
screws
4 Move the field diaphragm image to the center of
the view-field by turning the two condenser
centering screws on the supporting pillar for
dia-Illuminator.
5 Place the 50X objective into the optical path.
Field diaphragm image
View-field
Move the field diaphragm image into the center of
view-field. Adjust its size to match the view-field.
Figure 3.23-4
6 Adjust the size of the field diaphragm image to
closely match the size of the view-field, by turning
the field diaphragm dial on the supporting pillar for
dia-Illuminator.
7 Move the field diaphragm image to the center of
the view-field by turning the two condenser
centering screws on the supporting pillar for
dia-Illuminator.
Adjusting the field diaphragm
59
Chapter 3
3.23.4
Operation Details
Adjusting the aperture diaphragm (for the diascopic illumination)
The aperture diaphragm controls the numerical
aperture of the illumination system, and is closely
related to the resolution of the optical image, the
brightness, the contrast and the depth of focus.
Aperture
diaphragm
lever
Objective pupil
plane
Aperture diaphragm
image
Adjust the size of the aperture diaphragm image to
70-80% the size of the objective pupil plane.
Figure 3.23-5
3.23.5
Adjusting the aperture diaphragm
When the size of the aperture diaphragm is stopped
down, the resolution and the brightness are reduced
while the contrast and the depth of focus are
increased. However, when the size of the aperture
diaphragm is increased, the resolution and the
brightness are increased while the contrast and the
depth of focus are reduced. The resolution, the
brightness, the contrast and the depth of focus are
related to one another and cannot be changed
independently. Set the conditions to fit the
requirements of the sample and the intended
application.
Adjust the aperture diaphragm while looking at the
actual diaphragm image. Turn the aperture
diaphragm lever counter-clockwise to close the
aperture, or clockwise to open the aperture. Adjust
the aperture diaphragm so that the size of the
diaphragm image is at 70-80% of the size of the
objective pupil.
Diascopic illumination filter
Up to four filter sliders can be attached to the
supporting pillar for dia-Illuminator 100W.
Attach appropriate filters (φ45 mm) to the filter sliders.
(See Step 6, 4.15.2.)
Filter
sliders
Figure 3.23-6
Filter slider
60
Chapter 3
Operation Details
Filter list
Filter
Description
ND2
(Neutral Density
filter)
Adjusts the brightness for general microscopy or photomicroscopy. Reduces the light
intensity to 1/2. Dims the quantity of light to 1/2 (Transmittance: approx. 50%).
ND16
(Neutral Density
filter)
Adjusts the brightness for general microscopy or photomicroscopy. Dims the quantity
of light to 1/16 (Transmittance: approx. 6.3%).
GIF
(Green Interference
filter)
Improves the contrast for microscopy under a monochrome light or when performing
monochrome imaging.
NCB11
(Neutral Color
Balancing filter)
Corrects the color temperature for general microscopy or filming by daylight color TV
camera photography. Color reproduction is best when this filter is inserted into the
optical path, and the lamp voltage is set to the same display as the lamp rating.
Remove from optical path when performing monochrome imaging.
3.23.6
Condenser refocusing clamp
Form the field diaphragm image on the sample
surface by turning the condenser focus knob. Turn
the condenser refocusing clamp clockwise to the limit
to mark this position.
Condenser
refocusing
clamp
When the condenser is elevated to change the
sample, it can easily be brought back down to the
initial position (at which the field diaphragm image is
formed) by turning the condenser focus knob to the
limit. The condenser refocusing clamp has a range of
motion of 13 mm.
Condenser
focus knob
Condenser
focus knob
Figure 3.23-7
3.23.7
Condenser refocusing clamp
Condenser mount rotation
The condenser mount can be turned by loosening the
condenser mount rotation clamp screw.
Condenser mount
Condenser mount rotation
clamp screw
Figure 3.23-8
Condenser mount rotation
61
Chapter 3
3.23.8
Operation Details
Polarizer slider (T-P2), λ plate (TI-DIC)
When performing the simplified polarization microscopy under the diascopic illumination, mount the polarizer
slider on the condenser mount for the supporting pillar for dia-Illuminator 100W.
Push in the slider, and the polarizer enters the optical path.
If you loosen the rotation clamp screw and slightly turn the polarizer, the image contrast changes.
To perform the sensitive color microscopy, pull out the dust-proof plate from the polarizer slider to attach the
TI-DIC λ plate.
Clamp
Polarizer
Rotation clamp screw
Rotating section
Dummy hole
Polarizer rotation lever
Dial scale (5° intervals)
White index mark
Dust-proof plate/λ plate
slider
Fixing ring
Condenser turret
White index mark
Aperture diaphragm lever
Condenser lens
Figure 3.23-9
T-P2 polarizer slider
■ Adjusting the polarizer direction
White index mark
1 Focus on the condenser and bring it to the center
by following the instruction in 3.23.2.
2 Select the 50X objective and focus on the sample.
3 Move the stage to remove the sample out of the
view-field. In its place, bring an almost dust-free
area into the view-field. (There must be no dust in
the whole view-field.)
4 Remove an eyepiece from the binocular part to
attach the centering telescope.
(To observe the objective pupil, turn the eyepiece
while pressing the flange on the centering
telescope.)
5 Place the analyzer of the MA200 main body into
the optical path.
6 Push in the slider, and the polarizer enters the
optical path. Confirm that the white index mark on
the polarizer is positioned at the center of the dial
scale. (If it is not, adjust it as described in “4.
Assembly.”)
62
Chapter 3
Mount
rotation clamp
screw
Operation Details
7 Loosen the condenser mount rotation clamp
screw. Hold the condenser turret and turn both the
condenser and the polarizer at the same time.
Tighten the mount rotation clamp screw to fix it at
the position where a dark cross appears in the
objective pupil.
Be careful not to accidentally loosen the mount
rotation clamp screw during microscopy.
Dark cross that appears in the objective pupil
Figure 3.23-10
Adjusting the polarizer direction
63
Chapter 3
3.24
Operation Details
Using the Supporting Arm (MA2-MP)
The supporting arm is designed to mount Nikon camera control unit DS-L2.
The Supporting Arm for DS-L2 is equipped with a one-touch release. If you pull up the mount arm
attaching/detaching knob, the DS-L2 can be attached or removed from the supporting arm.
Mount arm
attaching/detaching knob
Mount arm
Rotation
torque
nuts (x2)
Supporting
arm
The rotation torque can be adjusted depending on the
tightness of the docking section of the mount arm to
keep the DS-L2 steady, while achieving more
comfortable operation for angle adjustment. Turn the
two nuts on the docking sections with the supplied
tool and control each rotation torque for the
horizontal/vertical direction.
For using the DS-L2, refer to the instruction manual
supplied with the DS-L2.
DS-L2
DS-L2 attached to the supporting arm
Figure 3.24-1
Supporting arm
64
Chapter 3
3.25
Operation Details
Using the DS Camera Control Unit (DS-L2)
Once you connect the DS Camera Control Unit DS-L2 with the DS camera attached to the vertical tube on
MA200 or the back port using the specified cable, you can operate the DS camera. Additionally, by
connecting MA200 with DS-L2 via USB cable, the following functions are available on the DS-L2.
• Displaying the microscope info menu
• Measurement corresponding to the magnification value of the microscope
• Recording the microscope info while saving the image
3.25.1
Procedure for turning on the power
1 Check that the MA200 main body and the DS-L2 are connected correctly. (Refer to “Cable Connection for
the DS-L2 and MA200” in 4.16.)
2 Set the power switch on the MA200 main body to the “|” side to turn it on.
3 Push in the power switch on the DS-L2 to turn it on.
3.25.2
Displaying the microscope info menu
Right-click on the mouse over the upper-screen of the DS-L2 to display the microscope info menu. (Refer to
Figure 3.25-1.)
Magnification/NA value
of the objective
■
The microscope info menu buttons
: Closes the microscope info menu.
Magnification value of
the intermediate
magnification unit
: Resizes the microscope info menu.
: Displays the objective info. (Refer to 3.25-2.)
To display the objective lens info on the DS-L2,
use the “MA200 Setup” in “MA200 Setup Tool”
supplied with MA200 to allow the user to
register the info from an external PC to the
microscope. For installing and operating
“MA200 Setup,” refer to the software manual of
“MA200 Setup Tool.”
Light source for the episcopic illumination
(EpiLamp) and brightness value (lamp voltage)
(If the motorized C-HGFIE HG Precentered Fiber
Illuminator is used as the light source for the
episcopic illumination (EpiLamp), the transmittance
of the ND filter for the fiber illuminator is indicated in
the brightness value area.)
to
:
Specify the nosepiece address to be placed into
the optical path. This button appears when the
motorized LV-NU5A is attached to MA200.
However, this is not applied to the manual
nosepiece. Press the button, then the revolving
nosepiece is automatically set to the nosepiece
address. The nosepiece address that is
currently placed into the optical path is framed in
red.
Figure 3.25-1 DS-L2 microscope info menu
65
Chapter 3
Operation Details
■
Nosepiece address
Name of the objective
NA value of the objective
WD value of the objective
Displaying the objective info
Press the
button, and the name and other info
are displayed of the objective at the nosepiece
address that is currently placed into the optical path
(see Figure 3.25-2). Close the objective info area with
the X button displayed on the right of the nosepiece
address.
Figure 3.25-2 Objective info
The nosepiece address of the motorized nosepiece that is mounted on the microscope can be controlled
from the microscope info menu on the DS-L2. To adjust the illumination of the lamphouse (LV-LH50PC)
with the DS-L2 connected, set [EXTERNAL ON/OFF] of the Internal/External brightness control
changeover switch on the rear of the MA200 main body to OFF (Internal mode), then adjust the
brightness control dial.
3.25.3
Measurement corresponding to the magnification value of the microscope
Once you calibrate the reference length, you can have a simple means of measuring distances of an image
on DS-L2.
When MA200 is connected with the DS-L2, you can save calibration values for each nosepiece address in
order to measure the length of a desired image. Additionally, when the intermediate magnification unit is
attached to MA200, the magnification value of the intermediate magnification unit is automatically multiplied
when measuring the length of the image.
For calibrating and measuring the length of an image, refer to the instruction manual supplied with the DS-L2.
3.25.4
Recording the microscope info while saving the image
When MA200 is connected with the DS-L2, the information on the objective, the intermediate magnification
unit, or the illumination for the episcopic microscopy, can be recorded as text data.
For setting log information or saving the image, refer to the instruction manual supplied with the DS-L2.
66
Chapter 3
3.25.5
Operation Details
Connecting with the DS camera head switcher (DS-SW: Camera switch BOX, optional)
If the DS-L2 is connected with the DS camera head switcher, two camera heads can be connected to the
DS-L2.
If the DS-L2 is connected with the DS-SW, the register tab appears on the DS-L2 microscope info menu. On
the register tab, the “Measurement interlock for the magnification value of the microscope” function can be set
to ON/OFF for each camera head.
If you check the checkbox, the measurement
corresponds to the optical magnification of
the microscope for the displayed image
captured by the camera connected to the
selected camera port of the DS-SW.
[REG.] tab
Sets if the optical
magnification value of
MA200 corresponds to
the port 1 on the camera.
Sets if the optical
magnification value of
MA200 corresponds to
the port 2 on the camera.
Figure 3.25-3 Registering the measurement interlock
For capturing the image and setting the camera, refer to the instruction manual supplied with the DS-L2.
67
4
Assembly
WARNING
WARNING and
CAUTION at the
• Before assembling the microscope, be sure to read the
beginning of this instruction manual and follow the instructions written therein.
• To prevent electrical shocks and fire, turn off the power switch (set the switch to the “c” side) and
unplug the power cord from the outlet when assembling the microscope.
CAUTION
• Be careful not to pinch your fingers or hands during assembly.
• Scratches or fingerprints on the lenses will adversely affect the microscopy image. Be careful not to
scratch or touch the lens surfaces. If lenses are contaminated with fingerprint or such, clean them
according to the procedure described in “6. Care and Maintenance.”
• This product is a precision optical instrument. Handle it carefully and do not subject it to a strong
physical shock. In particular, objectives may loose accuracy when exposed to even a weak physical
shock.
■ Required tools
• Two hexagonal screwdrivers (2 mm) (supplied with the microscope)
• Hexagonal wrench (3 mm) (supplied with the microscope)
• Hexagonal wrench (5 mm)
• Filter replacing tool (supplied with the microscope)
When these tools are not used, place them in the tool holder at the right-end of the top-surface of the product.
■ Installation location
This product is a precision optical instrument. The usage or storage in an inappropriate environment may
result in malfunctions or poor performance. Consider the following factors when selecting an installation
location:
• Avoid a brightly lit location, such as exposed to direct sunlight or directly under a room light. If there is
excessive ambient light, the image may not clearly visible.
• Always install the microscope with a surrounding clear area of 10 cm or more.
• Install the microscope in a location that is free from considerable dust or dirt.
• Install the microscope on a flat surface with little vibration.
• Install the microscope on a sturdy desk or table that is able to bear the weight of the instrument.
• Do not install the microscope in a hot and humid location.
• Arrange a layout that allows easy removal of the power cord from the inlet of the product in the event of an
emergency.
• For details about the operating environment and storage environment, refer to “7. Specifications.”
68
Chapter 4
Assembly
■ Combination of the illuminator and the light source
Use this product with a specified light source. The specified light source devices are as follows:
For the episcopic illumination
• Lamphouse
Nikon 12V 50W Precentered Lamphouse (model name: LV-LH50PC)
• Lamp
Nikon 12V 50W longlife halogen lamp (model name: LV-HL50W), or 12V 50W shortlife halogen lamp from
other manufacturers (model name: OSRAM HLX 64610, OSRAM HLX 64611, or PHILIPS 7027).
For the diascopic illumination
• Lamphouse
Nikon 12V 100W Precentered Lamphouse LC (model name: D-LH/LC)
• Lamp
Nikon 12V 100W halogen lamp (model name: OSRAM HLX 64623 or PHILIPS 77241).
• TI-PS 100W power supply
If you wish to buy these lamps, please contact your nearest Nikon representative.
69
Chapter 4
4.1
Assembly
About the System
Filter
(GIF, NCB11, ND2, ND16)
T-DIC λ plate
D-LH/LC
lamphouse
+ 12V 100W
halogen lamp
T-P2 polarizer
TI-C system
condenser
MA2-DP supporting pillar
for dia-Illuminator 100W
ELWD-S condenser
TI-C-LWD
condenser
lens
C-mount camera
C-mount
adapter
MC TMD2 ELWD
condenser lens
C-mount adapter
LV-TV
adapter
Eyepiece
MA2-MC
intermediate
magnification unit
TI-PS 100W
power supply
DS-L2
monitor
MA2-MP
supporting arm
for DS-L2
MA2-TI3 trinocular
eyepiece/Y-TB
binocular eyepiece
tube
C-ER
eyelevel riser
MA2-FL
fluorescent Unit
(G, B, BV, V)
Sample holder
MA2-λP
λ plate
MA2-SR
rectangular stage
MA2-PA/MA2-UPA
polarizer/analyzer
unit
MA200 main body
12V 50W
halogen lamp
MA2-GR grain scale slider
MA2-MR scale slider
EPI Objective
D-ND6
revolving
nosepiece
LU nosepiece
adapter M32-25
D-NI7
MA2-NUI5
revolving revolving nosepiece
nosepiece
LV-LH50PC
Lamphouse (for
the episcopic
illumination)
BD objective
C-LHGFI
HG lamp
LV-HGFA
HG fiber
adapter
LV-NU5A motorized
nosepiece
C-HGFIF
HG fiber
D-LP λ plate slider
D-DA analyzer slider
LV-DIC/LV-DIHC slider
(Senarmont method)
LV-NCNT2 Nosepiece
Controller 2
L-DIC/L-DIHC slider
(single NR method)
70
C-HGFI/C-HGFIE HG
Precentered Fiber Illuminator
(for the episcopic illumination)
Chapter 4
4.2
Assembly
Combination List for the Unit
Combinations for each microscopy provided for MA200.
: All selectable or available.
c: Selections limited. Only the unit under this symbol is available.
/: Unselectable or unavailable.
n: Notes on combinations.
Table 4.2-1
Unit name
Model
Combination List for the Unit
Simplified
Simplified
BF
DF
DIC
BF
polarization
polarization
microscopy microscopy
microscopy
microscopy
Epi-fl
microscopy
microscopy
under the
under the
under the
under the
microscopy
under the
under the
epi
epi
epi
dia
epi
dia
illumination illumination
illumination
illumination
illumination
illumination
Microscope
MA200

Stage
MA2-SR rectangular stage (sample
holder supplied)

Light source
Eyepiece tube
Eyepiece
LV-LH50PC Lamphouse (specified light
source, refer to “7. Specifications”)





/
/
C-HGFI/C-HGFIE HG Precentered
Fiber Illuminator





/
/
MA2-DP supporting pillar for
dia-Illuminator 100W under diascopic
illumination
/
/
/
/
/





MA2-TI3 trinocular eyepiece tube

Y-TB binocular eyepiece tube

All types

LV-NU5A motorized nosepiece
Revolving
nosepiece
MA2-NUI5 revolving nosepiece

D-ND6 revolving nosepiece
c

5 revo.
D-NI7 revolving nosepiece
CFI LU Plan FLUOR EPI
CFI L Plan EPI

1
2
LU BD
objective
c

1
2
Objective
CFI LU Plan FLUOR BD
c
5 revo.
6 revo.
LU EPI
objectives
LU BD
objective
1
2
3
LU EPI
objectives
LU BD
objective
1
2
3
c

1
2

1
2
c
Polarizer/analyzer
unit
MA2-PA/MA2-UPA unit
/
/


/
/

Polarizer
T-P2 polarizer
/
/
/
/
/
/
D-DA analyzer
/
/
/
/
/
/


MA2-λP λ plate
/
/


/
/
D-LP λ plate
/
/
/
/
/
/
TI-DIC λ plate
/
/
/
/

/
/
/

/
/
/
/
/
/
/
/
/
/
/
/
/
/

/
/
Analyzer
λ plate
DIC prism (single
NR method)
L-DIC/L-DIHC slider
DIC prism
(Senarmont
method)
LV-DIC/LV-DIHC slider
Fl unit
MA2-FL unit (G, B, BV, V)
Scale
MA2-GR/MA2-MR slider

Intermediate
magnification
MA2-MC intermediate magnification unit

Monitor stand
MA2-MP supporting arm for DS-L2

Sample holder
Multiple types

Eyelevel riser
C-ER eyelevel riser

Camera adapter
LV-TV TV adapter or C-mount adapter

71
6

7
/
4
/

5
Chapter 4
Assembly
1: For EPI objective on 5 revo., “LU nosepiece adapter M32-25” is required.
2: BD objective is unavailable on 6 revo., 7 revo.
3: The CFI L Plan EPI objective lens cannot be used for DIC microscopy under the epi-illumination, epi-fl microscopy.
4: D-DA analyzer is attachable for 6 revo. only.
5: D-LP λ plate is attachable for 6 revo. only.
6: L-DIC/L-DIHC slider is attachable for 5 revo. only.
7: LV-DIC/LV-DIHC slider is attachable for 6 revo. only.
72
Chapter 4
4.3
Assembly
Network Connection
4.3.1
Unit system capable of establishing network
The table below shows a unit system capable of establishing network on a PC or the DS-L2 connected to
MA200 via the USB cable.
MA2-DP supporting
pillar for dia-Illuminator
100W
Camera cable
D-LH/LC Lamphouse
Power cord
DS-U2/DS-L2 [Note 1]
Power cord
Power supply
AC adapter
USB
cable
AC adapter/power cord
exclusive for your PC
Camera cable
Intermediate
magnification unit
PC
Power cord
LV-LH50PC Lamphouse
DS-L2
AC adapter
Power cord
Fiber
adapter
Power
cord
USB cable
Signal cable
Power cord
AC adapter
MA200
main body
HG fiber
C-HGFI
HG Precentered Fiber Illuminator
RS232C cross cable
Nosepiece
Controller 2 [Note 2]
Power
cord
RS232C cross cable
C-HGFIE
HG Precentered Fiber Illuminator
[Note 1] When the DS-L2 is connected, the MA200 cannot be controlled.
[Note 2] For use with LV-NU5A motorized nosepiece.
73
Chapter 4
4.3.2
Assembly
Viewing user control options and status displays on a PC or the DS-L2
The table below shows user control options and status displays of a unit that can be controlled on a PC or the
DS-L2 connected to MA200 via the USB cable.
The “/” indicates external control/status display is unavailable.
To control MA200 on a PC or to display the current status of the MA200 on a PC, use the “SDK” in the
supplied “MA200 Setup Tool” software. For details on how to install the “SDK” on a PC, refer to the
software manual supplied with the “MA200 Setup Tool.”
74
Chapter 4
Table 4.3-1
User control options for the unit using the PC/DS-L2
PC
Unit
Control
DS-L2
Notes
/
---
Status
EXTERNAL:
Lamp lit/unlit,
Lamp lit/unlit,
ON (external lamp brightness lamp brightness
mode)
adjustment
adjustment
/
Lamp lit/unlit,
lamp brightness
adjustment
/
Lamp lit/unlit,
lamp brightness
adjustment
/
Lamp lit/unlit,
Lamp lit/unlit,
lamp brightness lamp brightness
adjustment
adjustment
/
/
/
Light source
C-HGFI HG Precentered
Fiber Illuminator
Status
Brightness
control dial
Control
LV-LH50 PC
Lamphouse EXTERNAL:
OFF
(internal
mode)
C-HGFIE HG Precentered
Fiber Illuminator
Changing the
shutter
open/close, ND
(Neutral
Density)
1
Shutter
open/close, ND
(Neutral
Density)
MA2-DP supporting pillar
for dia-Illuminator 100W
/
/
LV-NU5A motorized
nosepiece
Rotation of the
revolving
nosepiece
2
/
Rotation of the
revolving
nosepiece
3
/
Do not use the C-HGFI when
the LV-NU5A motorized
nosepiece is mounted.
Shutter
open/close, ND
(Neutral
Density)
/
To connect the C-HGFIE with
the MA200 main body, use
the RS232C cross cable.
When attaching the C-HGFIE
to the microscope with the
LV-NU5A motorized
nosepiece mounted, RS232C
cross cable must be used for
their connection to control the
shutter according to the
rotation of the motorized
nosepiece. If you fail to make
this connection, a fire of light
may enter in the view-field at
the rotation of the motorized
nosepiece.
/
/
To use the MA2-DP for the
diascopic illumination, set the
brightness control dial to OFF.
/
Address for the
revolving
nosepiece
Connect the Motorized
Nosepiece Controller 2 with
the MA200 main body using
the RS232C cross cable.
MA2-NUI5 revolving
nosepiece
/
Address for the
revolving
nosepiece
D-ND6 revolving nosepiece
/
Address for the
revolving
nosepiece
/
Address for the
revolving
nosepiece
---
D-NI7 revolving nosepiece
/
Address for the
revolving
nosepiece
/
Address for the
revolving
nosepiece
---
Intermediate
magnification
3
Address for the
revolving
nosepiece
/
---
MA2-MC intermediate
magnification unit
/
Magnification of
the MA2-MC
/
Magnification of
the MA2-MC
---
Objective
information
Revolving nosepiece
Assembly
/
Address for the
revolving
nosepiece
---
Registering information on
the objective
Objective info
Objective info
/
Objective info
Refer to “4.3.3 Registering
information on the objective.”
1: Shutter open/close or ND select can be controlled using the remote controller (C-HGFIE-C HG controller) exclusive for the C-HGFIE.
2: The shutter open/close or ND select can be controlled if the remote controller (C-HGFIE-C HG controller) exclusive for the C-HGFIE
is used.
3: The rotation of the revolving nosepiece can be controlled on the Motorized Nosepiece Controller 2.
75
Chapter 4
4.3.3
Assembly
Registering information on the objective
You can register the information to be checked on a PC or the DS-L2 with the “MA200 Setup” in the supplied
“MA200 Setup Tool” software. For details on how to install the “MA200 Setup” on an appropriate device, refer
to the software manual supplied with “MA200 Setup Tool.”
4.3.4
Procedure for turning on the power switch
To establish an appropriate connection between MA200 and an external device or to use the product safely,
turn on the power as follows.
■ For using the C-HGFIE HG Precentered Fiber Illuminator (motorized type)
1 Set the power switch on the fiber illuminator to the “|” side and wait until it starts up.
2 Set the power switch on the MA200 main body to the “|” side to turn it on.
3 To turn off the fiber illuminator, set the power switch to the “c” side in the sequence of the fiber illuminator,
the MA200 main body.
■ For using the Nosepiece Controller 2
1 Set the power switch on the Nosepiece Controller 2 to the “|” side to turn it on.
2 Set the power switch on the MA200 main body to the “|” side to turn it on.
■ For using the C-HGFIE HG Precentered Fiber Illuminator (motorized type) and the Nosepiece Controller 2
1 Set the power switch on the fiber illuminator to the “|” side and wait until it starts up.
2 Set the power switch on the Nosepiece Controller 2 to the “|” side to turn it on.
3 Set the power switch on the MA200 main body to the “|” side to turn it on.
4 To turn off the dia pillar illuminator, set the power switch to the “c” side in the sequence of the fiber
illuminator, the MA200 main body, the Nosepiece Controller 2.
■ For using the DS-L2/DS-U2
1 Set the power switch on the MA200 main body to the “|” side to turn it on.
2 Push in the power switch on the DS-L2 or DS-U2 to turn it on.
■ For using the MA2-DP Supporting Pillar for Dia-Illuminator 100W under the diascopic illumination
1 Set the brightness control dial on the MA200 main body to OFF.
2 Set the power switch on the power supply (TI-PS100W) to the “|” side to turn it on.
3 Set the power switch on the MA200 main body to the “|” side to turn it on.
76
Chapter 4
4.4
Assembly
The LV-LH50PC Lamphouse and the Lamp
CAUTION for heat
Do not touch the lamp and the lamphouse while the lamp is on or for thirty minutes after it has been
turned off.
CAUTION
• To prevent electrical shock and damage to the microscope, always turn off the power switch (set the
switch to the “O” side) and unplug the power cord from the outlet before attaching or detaching the
lamphouse or replacing the lamp.
• To prevent burn injury, allow the lamp and the lamphouse to cool down completely (for at least 30
minutes after the lamp is turned off), before replacing lamps or attaching/detaching the lamphouse.
• Never touch the glass surface of the lamp with bare hands. Doing so will cause fingerprints, grease,
etc., to generate ghost images on the lamp surface, reducing the illumination. If you do get any
fingerprints or dirt on the lamp, wipe them clean.
• Make sure the lamphouse cover is securely fitted to the lamphouse after replacing lamps. Never
turn on the lamp with the lamphouse cover removed.
• When you dispose of the replaced lamp, do not break it. Instead, dispose of the used lamp as
industrial waste or dispose of it according to the local regulations and rules.
4.4.1
Attaching the lamphouse
1 Fully loosen the clamp screw on the upper side of
the lamphouse connecting sleeve by using the
supplied hexagonal screwdriver.
(1)
(2)
(4)
2 Mount the lamphouse to the connection port on
the rear of the product and insert the lamphouse to
the limit.
3 Using the hexagonal screwdriver, tighten the
clamp screw on the upper side of the connector of
the lamphouse to secure it.
4 Plug the cable coming from the lamphouse into
the lamphouse connector [LAMP DC12V 50W] on
the rear of the product and tighten the ring of the
connector to secure the connection.
77
Chapter 4
4.4.2
Assembly
Replacing the lamp
1 Loosen the lamphouse cover clamp screw using
the supplied hexagonal wrench.
2 Remove the lamphouse cover.
(1)
3 Press the lamp clamp levers and remove the old
lamp.
(3)
(2)
4 With the lamp clamp levers pressed, insert the
electrodes of a new lamp into the holes of the
socket. Insert the lamp to the limit, and release the
lamp clamp levers to secure the lamp.
Be careful not to touch the glass surface of the
lamp with bare hands.
(4)
When releasing the lamp clamp levers, take care
so that the lamp does not tilt.
5 Reattach the lamphouse cover and secure it by
tightening the clamp screw.
*
The lamp exclusive for this microscope is the LV-HL50W 12V 50W longlife halogen lamp. The 12V 50W
shortlife halogen lamp from other manufacturers (OSRAM HLX 64610, OSRAM HLX64611, PHILIPS
7027) may also be used. If you wish to buy these lamps, please contact your nearest Nikon representative.
4.5
Revolving Nosepiece
The following instructions are applied to all revolving nosepieces to attach the microscope.
*
For using the MA2-NUI5, D-ND6, or D-NI7 manual revolving nosepiece, be sure to provide a cable
connection between the revolving nosepiece and the microscope.
*
For using the LV-NU5A motorized nosepiece, be sure to provide a cable connection for the LV-NCNT2
Nosepiece Controller 2.
4.5.1
Attaching the nosepiece
Hole to pull out the
nosepiece cable
Nosepiece clamp screw
1 Fully loosen the nosepiece clamp screw located
on the front of the mount using the supplied
hexagonal screwdriver.
2 Align the revolving nosepiece with the dovetail at
the mount and insert it from the front on the
microscope to push it into the limit.
3 Fix the revolving nosepiece with the nosepiece
clamp screw.
4 Adhere the address sticker for the revolving
nosepiece supplied with the MA200 main body.
Look down at the revolving nosepiece, then
adhere the address sticker clockwise in ascending
order starting with the revolving address1.
Nosepiece cable
I
Sticker of the nosepiece
address 1 on the
MA2-NUI5 revolving
nosepiece
The “|” sticker is adhered on the address 1 for the
MA2-NUI5 revolving nosepiece. (See the figure on
the left.)
5 Pass the nosepiece cable through the hole on the
inner-rear of the microscope.
78
Chapter 4
4.5.2
Assembly
Cable connection for the manual revolving nosepiece and the MA200
Connect the cable of the revolving nosepiece to the connector on the microscope.
1 Loosen the rear-panel clamp screw on the
microscope with the supplied hexagonal
screwdriver to remove the rear-panel.
2 Connect the cable of the revolving nosepiece to
the connector on the microscope.
Connect the cable of the MA2-NUI5 revolving
nosepiece to the round connector on the
microscope.
Square connector
Round connector
*
Connect the cable of the D-ND6/D-NI7 revolving
nosepiece to the square connector.
3 Secure the clamp screw to reattach the
rear-panel.
The address information for the revolving nosepiece is displayed on the MA200 front display via cable
connection. Additionally, the address information can be output to a device including a PC or the DS-L2 via
USB connection.
4.5.3
Connecting the motorized nosepiece and the Nosepiece Controller 2
Make sure to turn off the motorized Nosepiece Controller 2 to attach or remove the signal cable.
1 Loosen the rear-panel clamp screw on the
microscope with the supplied hexagonal
screwdriver to remove the rear-panel.
2 Remove the cap from the signal cable hole on the
rear-panel.
3 Pass the signal cable for the LV-NCNT2
Nosepiece Controller 2 through the hole of the
rear-panel, then connect it to the cable of the
revolving nosepiece.
4 Secure the clamp screw to reattach the
rear-panel.
5 Connect the signal cable to the motorized
nosepiece connector on the LV-NCNT2.
6 Connect the LV-NCNT2 to the [NCNT] connector
on the MA200 main body using the RS232C cross
cable.
79
Chapter 4
4.6
Assembly
Stage
• To attach or remove the stage, hold the stage securely to prevent from being fallen.
• To remove the stage from the microscope with the supporting pillar for dia-Illuminator 100W
mounted, be sure that the stage does not come into contact with the condenser, etc.
• A cross roller guide is provided to move the stage in the X/Y direction. The Y direction movement
rack is located at the bottom of the stage. Do not touch the rack during your operation, as you may
get injured.
1 Place the stage on the stage mount so that the
stage handle is positioned at the right front. Align
three screw holes on the stage with the
corresponding screw holes on the mount part.
2 Insert three hexagonal socket head bolts (M6 x 20
mm) and secure them with the supplied hexagonal
wrench (5 mm). Attach the stage so that its top
surface is located in a horizontal position.
Y direction
movement rack
4.6.1
Sample holder
The MA2-SR rectangular stage is supplied with a sample holder (outer diameter: φ108 mm, Teardrop-shaped
aperture: φ22 mm, with a detachable sample clip). Attach the sample holder onto the mount part on the
center-stage. Other types of sample holders are also available (see 3.8.1). Remove the standard sample
holder from the center-stage to attach a desired sample holder instead. Additionally, the rotation knob is
supplied with the sample holder (except MA-SRSH1) and screw the knob into the hole on the ring.
80
Chapter 4
4.7
Assembly
Objectives
1 Remove the sample holder from the stage.
2 Screw the objectives into the socket of the
revolving nosepiece securely through the stage
hole.
Attach the objectives so that the magnification
increases when the revolving nosepieces are
turned clockwise as viewed from above.
■ LU nosepiece adapter M32-25
To use the EPI objectives with small screw diameter while the quintuple revolving nosepiece is in use, screw
the LU nosepiece adapter M32-25 into the revolving nosepiece in advance.
4.8
Eyepiece Tube
Fully loosen the eyepiece tube clamp screw on the
upper-left with the supplied hexagonal screwdriver.
Insert the dovetail on the eyepiece tube into the
circular groove on the attaching part to secure the
eyepiece tube with clamp screw.
Eyepiece tube
clamp screw
Hold the eyepiece tube by hand when loosening
the clamp screw to prevent a sudden
disconnection and falling.
4.9
Eyepieces
Attach eyepieces of the same magnification and the
same view-field number.
There are positioning protrusions on the binocular
part sleeve of the eyepiece tube. Align the notches of
the eyepiece with the protrusions on the sleeve and
slide the eyepiece into the eyepiece socket.
81
Chapter 4
4.10
Assembly
Grain Scale Slider/Scale Slider
1 Remove the dust protection cover from the scale
slider slot on the bottom-front of the microscope.
Be sure to face the indication on the slider to the
correct direction. Insert the slider in the direction
shown in the figure.
2 Securely insert the slider to the limit.
• When removing the scale slider, do not touch
or press hard against the glass. It may cause
damage to the micrometer reticle.
• Remove the dust on the glass with a soft
blower or brush.
4.11
Filters
You can attach the φ25-mm, up to 3-mm thick filter on the optional slot for the filter turret 1 or 2.
When handling filters, put on gloves and do not touch the surface of filters with bare hands. And be
careful not to let dust or fingerprints get on them.
1 Loosen the clamp screws (2) for the filter turret
with the supplied hexagonal screwdriver.
2 Remove the filter turret from the microscope.
3 Remove the filter holding ring from the optional
slot with the supplied filter replacing tool.
4 Insert the desired filter into the optional slot to
screw the holding ring back securely.
5 Attach the filter turret to the microscope.
82
Chapter 4
4.12
Assembly
Various Sliders for Attaching the Revolving Nosepiece
To insert a slider, be sure to attach the objective in advance.
4.12.1
L-DIC/L-DIHC slider (single NR method)
For using the DIC slider in single NR method, attach the MA2-NUI5 revolving nosepiece or LV-NU5A
motorized nosepiece.
Sliding limit groove
1 Loosen the DIC slider limit screw on the
nosepiece using a hexagonal screwdriver.
2 Remove the slot from the revolving nosepiece to
insert the DIC slider.
3 Screw in the DIC slider limit screw.
When removing the DIC slider from the nosepiece,
fully loosen the DIC slider limit screw using a
hexagon screwdriver, and then pull out the slider.
Prism setting dial
Prism movement knob
Quintuple nosepiece
DIC slider limit screw
83
Chapter 4
4.12.2
Assembly
LV-DIC/LV-DIHC slider (Senarmont method)
Attach the DIC slider in Senarmont method to the D-ND6 revolving nosepiece.
DIC slider slot
1 Select the slider (A or B) corresponding to the
objective to be used (refer to 3.16)
2 Insert the slider into the slot corresponding to the
position of the objective. Push the slider into the
limit to place the DIC prism in the optical path.
3 If you do not want to use the DIC slider, remove it
from the revolving nosepiece.
Sextuple nosepiece
DIC slider
4.12.3
D-DA analyzer slider, D-LP λ plate
Attach the D-DA analyzer and the D-LP λ plate to the D-ND6 revolving nosepiece.
Two slots are provided for the revolving nosepiece.
Insert the analyzer slider into the slot (indicated with
“A”) far from the objective, and insert the λ plate into
the slot (indicated with “λ”) near the objective.
Insert these two sliders with their nameplates down.
When the sliders hit the second click-stop position,
the analyzer or D-LP λ plate enters the optical path.
Pull them out to the first click-stop position, and the
dummy hole enters the optical path.
*
Analyzer slider
λ plate
Sextuple nosepiece
84
These sliders are designed to perform the
simplified polarization microscopy under the
diascopic illumination. Insert the dummy slider into
the slot for other microscopy as these sliders are
not required.
Chapter 4
4.13
Assembly
Polarizer/analyzer Unit and Fluorescent Unit
1 Loosen the front-cover fixing bolt with the supplied
hexagonal screwdriver to remove it. Turn to loosen
the BD field changeover lever counter-clockwise,
then remove the lever and front-cover from the
main body.
Fluorescent unit
Polarizer/analyzer unit
λ plate
2 Insert the unit into the slot inside the microscope.
Push the unit into the second click-stop position,
the polarizer/analyzer or fl filter enters the optical
path. Pull out the unit until it hits the click-stop
position, the polarizer/analyzer or the fl filter can
be removed from the optical path.
3 Remove the dust protection cover from the front
cover to hide the operation port, then return the
front cover to the original place to fix it with the
bolt.
To perform the epi-fl microscopy using the
C-HGFI/C-HGFIE HG Precentered Fiber
Illuminator as the external light source, screw the
compensation filter supplied with the fiber
adapter into the fluorescent unit. (Refer to
4.15.1.)
4.13.1
MA2-λP λ plate
The MA2-λP λ plate can be attached to the
MA2-PA/MA2-UPA polarizer/analyzer unit. Insert the
MA2-λP slider from the front as illustrated.
85
Chapter 4
4.14
Assembly
Intermediate Magnification Unit
1 Remove four bolts to take out the cover on the
attaching part for intermediate magnification unit
with the supplied hexagonal screwdriver.
2 The cables are secured by the cable keeper inside
the opening as the figure shows. Remove the
cable from the cable keeper, then connect the
cable to the connector on the intermediate
magnification unit.
*
Cable
Cable keeper
The cable keeper is attached to MA200 with a
double-side tape. Be sure to remove the keeper
before attaching the intermediate magnification
unit.
3 Insert the intermediate magnification unit to the
opening (with the MAGNIFICATION indications
up) to the limit, then screw the four bolts back into
the microscope securely.
Opening for the intermediate magnification unit
86
Chapter 4
4.15
Assembly
External Light Source
You can attach the HG precentered fiber illuminator (for the episcopic illumination) or the supporting pillar for
dia-Illuminator 100W as an external light source.
CAUTION
• To use an external light source, carefully read the instruction manual and make sure to follow the
instructions.
• To assemble and connect parts, check that the power supplies for the light source and the product
are turned off and that the power cord is unplugged from the outlet.
4.15.1
C-HGFI/C-HGFIE HG Precentered Fiber Illuminator
WARNING
• An HG precentered fiber illuminator emits very strong light including ultraviolet light that is harmful to
the eyes and skin. Never turn on the power for the light source before completion of assembling and
connecting parts.
• To prevent a fire of flash
To attach the HG precentered fiber illuminator to the microscope with the LV-NU5A motorized
nosepiece mounted, be sure to use the motorized HG precentered fiber illuminator. Because it is
necessary to control the shutter according to the rotation of the motorized nosepiece, connect the
fiber illuminator and [HGFIE] connector on the microscope using the RS232C cross cable supplied
with the C-HGFIE. If you fail to make this connection, a fire of light may enter in the view-field at the
rotation of the motorized nosepiece.
1 If mounted, remove the LV-LH 50PC Lamphouse.
Fiber adapter
clamp screw
2 Fully loosen the fiber adapter clamp screw using
the hexagonal screwdriver.
Fiber clamp
screw
HG fiber
3 Mount the fiber adapter onto the attaching part of
the LV-LH 50PC. Push the adapter into the limit
and fix it with the fiber clamp screw.
4 Insert the HG fiber tip through the hole of the fiber
adapter, and then tighten the fiber clamp screw to
fix the HG fiber using the hexagonal screwdriver.
5 Connect the HG fiber to the HG fiber port on the
fiber illuminator.
Fiber adapter
Compensation filter
attachment
Compensation
filter
MA2-FL fluorescent unit
6 Screw the compensation filter supplied with the
fiber adapter into the compensation filter
attachment on the MA2-FL fluorescent unit.
This filter is designed for adjusting color balance
and brightness. If you fail to attach it to the
fluorescent unit, an extremely strong light enters
the view-field during the bright-field microscopy.
Be sure to attach it.
*
87
For information about connecting the HG fiber,
refer to the instruction manual for the
C-HGFI/C-HGFIE HG Precentered Fiber
Illuminator.
Chapter 4
Assembly
*
4.15.2
For the C-HGFIE motorized fiber illuminator,
connect the C-HGFIE and the [HGFIE] connector
on the MA200 using the RS232C cross cable.
Attaching the MA2-DP supporting pillar for dia-Illuminator 100W
When working, hold the dia pillar illuminator to prevent it from falling.
Loosen clamp screws (2) using the supplied
hexagonal screwdriver, and remove the cover from
the attaching part of the supporting pillar for
dia-Illuminator 100W/supporting arm on the
top-surface of the microscope.
Mount the supporting pillar for dia-Illuminator onto the microscope.
Align the pins (2) on the lower-surface of the
supporting pillar for dia-Illuminator with the holes on
the attaching part. Using the supplied hexagonal
wrench (5 mm), secure the dia pillar illuminator by
tightening the four hexagonal socket head bolts (M6 x
20 mm).
Attach the condenser mount to the supporting pillar for dia-Illuminator.
1 Remove the fall-stop screw.
2 Attach the condenser mount by sliding it along the
dovetail on the supporting pillar for dia-Illuminator.
Slide the mount upward to the limit.
3 Using a hexagonal screwdriver securely tighten
the clamp screw on the right of the condenser
mount.
Condenser
mount
4 Attach the fall-stop screw.
Clamp screw
Fall-stop screw
88
Chapter 4
Assembly
Attach the condenser to the condenser mount.
You can attach the TI-C condenser turret (system condenser) or ELWD-S condenser to the condenser mount.
Additionally, attach the TI-C-LWD condenser lens or the MC TMD2 ELWD condenser lens to the system
condenser.
■
Preparation for attachment
Positioning pin
Positioning
groove
Condenser mount
rotation clamp screw
■
Condenser
clamp screw*
(within side hole)
Loosen the condenser clamp screw on the right side
of the condenser holder using a hexagonal
screwdriver.
*
Condenser
holder
The condenser clamp screw is located inside the
hole on the right side of the condenser holder. If
the condenser mount is shifted from the reference
position, the screw will not be visible in the hole.
In that case, loosen the condenser mount rotation
clamp screw, align the positioning groove on the
mount with the positioning pin on the condenser
holder by turning the mount, and then tighten the
condenser mount rotation clamp screw.
Condenser mount
Attaching the TI-C system condenser
1 Turn the condenser turret to the “A” position
(dummy hole cassette position for bright-field
microscopy).
Dovetail
Condenser
clamp screw
“A” position
System condenser
2 With the “A” label facing the front (towards the
user), insert the dovetail on the system condenser
into the bottom of the condenser holder, and
secure it by tightening the condenser clamp screw.
Attach the condenser turret by sliding it in from the
front.
3 Insert the condenser cassette into the condenser
turret, and secure it with two hexagonal socket
head bolts.
Up to five condenser cassettes can be attached to
the turret. Attach the cassettes so that their
numbering is increased by turning the turret
clockwise (as viewed from above).
Condenser
cassette
4 Screw in the condenser lens into the bottom of the
turret.
Condenser lens
89
Chapter 4
■
Assembly
Attaching the ELWD-S condenser
With the indication on the turret facing the front
(towards the user), insert the condenser turret into the
bottom of the condenser holder, and secure it by
tightening the condenser clamp screw.
Dovetail
Condenser
indication
position
Condenser
clamp screw
ELWD-S condenser
Attach the D-LH/LC Lamphouse to the supporting pillar for dia-Illuminator 100W.
Insert hexagonal
screwdriver and
tighten clamp screw.
1 Insert the lamphouse into the lamphouse mount at
the top of the supporting pillar for dia-Illuminator.
Lamphouse
clamp screw
Align the positioning pin on the dia pillar
illuminator with the groove on the cylinder of the
lamphouse.
2 Insert a hexagonal screwdriver into the hole on the
top of the supporting pillar for dia-Illuminator.
Secure the lamphouse by tightening the
lamphouse clamp screw.
3 Secure the lamphouse cable with the cable clamp
on the back of the supporting pillar for
dia-Illuminator.
The cable clamp is attached to the supporting
pillar for dia-Illuminator with the hooks on its sides.
To remove the cable clamp, push in the hooks
from the sides.
The cable clamp can hold up to four cables.
Cable clamp
90
Chapter 4
Assembly
Attach/replace the lamp.
Be sure to use the halogen lamp (OSRAM HLX64623 or PHILIPS77241) exclusive for the D-LH/LC
Lamphouse.
CAUTION for heat
Do not touch the lamp and the lamphouse while the lamp is on or for thirty minutes after it has been
turned off.
WARNING
• When replacing the lamp, turn off the power supply and unplug the power cord.
• The lamp and its surroundings will be hot while the lamp is on and immediately after it is turned off.
When replacing the lamp, wait approximately 30 minutes after turning off the lamp, and make sure
that the lamp has cooled down sufficiently.
• Do not touch the lamp glass with your bare hands. Fingerprints and other dirt on the lamp may result
in uneven illumination and reduce the service life of the lamp. Wear gloves when handling the lamp.
• Securely close the lamphouse cover after replacing the lamp. Never turn on the lamp with the cover
removed.
• When you dispose of the replaced lamp, do not break it. Instead, dispose of the used lamp as
industrial waste or dispose of it according to the local regulations and rules.
1 Insert a hexagonal wrench or a hexagonal
screwdriver into the hole on the top of the
lamphouse cover. Loosen the clamp screw and
remove the lamphouse cover.
Insert hexagonal wrench
and tighten clamp screw.
Clamp
screw
2 Press the lamp clamp levers and remove the used
lamp from the socket.
Lamphouse
cover
3 Insert a new lamp into the socket.
While pushing in the lamp clamp levers, push the
lamp electrodes (pins) into the pin holes in the
socket. Insert the lamp to the limit, and release the
lamp clamp levers.
Make sure the lamp is not tilted when the lamp
clamp levers are released.
4 Re-attach the lamphouse cover, and secure it by
tightening the clamp screw.
Lamp
Lamp clamp levers
Push in the lamp clamp lever to open the pin hole on
the socket. Remove the lamp while pushing the lever in.
Attach new lamp.
91
Chapter 4
Assembly
Attach the filter and the slider.
• Do not touch filters or other optical components with your bare hands.
• Latches at both ends of the filter slider are at their end point when sliding. When removing a filter
slider, you can slide it out by pushing up the latch on the opposite side of the tab with your finger to
release the filter slider. Applying undue force on the filter slider can break the latches.
1 Attach the desired filter to the filter slider.
Filter slider
(back)
Attach it from the back of the filter slider. The
mounting hole has three tabs to keep the filter
from falling. Only one tab can be moved to the
side. Move this tab aside, and attach the filter.
Filter
2 Affix a label indicating the filter type on the tab of
the filter slider.
Tab
3 Insert the filter slider into the slot of the supporting
pillar for dia-Illuminator.
The filter slider has latches that determine the limit
of slide operation. Push the latches up, and push
the filter into the slot.
You can insert up to four filter sliders.
You can insert filter sliders from the right or the
left. If they are all inserted from the same direction,
they will be difficult to handle, so you should insert
them alternately from the left and right.
Latches
Connect the TI-PS100W power supply.
POWER
switch
EXTERNAL/CTRL
switch
Connect the cable of the 100W diascopic illumination
lamp to the 12 VDC output connector on the power
supply. Secure the lamp cable with the lock ring.
Do not connect the lamp cable of 100W diascopic
illumination lamp to the lamp connector [LAMP
DC12V50W] on the MA200 main body. It is possible
to connect the cable to the connector because the
type of the cable connector is the same as the one on
the lamp connector. However, if you turn the
brightness control dial to the maximum, the light turns
dull being caused by inappropriate connection.
Connect the lamp cable of 100W diascopic
illumination lamp to the TI-PS100W power supply as
described above.
AC inlet
12 VDC/6 VDC
output connector
*
EXTERNAL/CTRL
connector*
92
Do not use the EXTERNAL/CTRL connector.
Chapter 4
Assembly
Attach the T-P2 polarizer slider.
Fixing ring
1 Remove the system condenser, if attached, from
the condenser holder to attach the T-P2 polarizer
slider.
Pin
2 Mount the polarizer slider on the condenser
mount, and align the positioning pin for the
polarizer slider with the groove on the condenser
mount. Turn the fixing ring to secure the polarizer
slider.
Groove
3 Loosen the rotation clamp screw, hold the
polarizer rotation lever, and turn the rotating
section to align the white index mark with the
center of the scale plate. (Be sure that the steel
ball on the tip of the clamp screw is in the groove
on the rotating section.) Tighten the rotation clamp
screw to secure the rotating section.
Condenser
mount
Rotation clamp
screw
Steel ball
and groove
Polarizer
rotation lever
White
index mark
4 Use a hexagonal screwdriver to loosen the
condenser mount rotation clamp screw on the left
side of the condenser holder. Turn the entire
polarizer to align the white index mark set in Step
3. with the white index mark on the condenser
holder. Tighten the mount rotation clamp screw to
secure the mount.
5 The polarizer can now be moved in and out of the
optical path by moving the slider.
White
index mark
Mount rotation
clamp screw
93
Chapter 4
4.16
Assembly
Supporting Arm (for DS-L2)
You can mount the camera control unit DS-L2 on the MA200 main body by attaching the supporting arm.
1 Loosen clamp screws (2) using the supplied
hexagonal screwdriver and remove the cover from
the attaching part of the supporting pillar for
dia-Illuminator 100W/supporting arm on
top-surface of the microscope.
2 Align the pins (2) on the lower-surface of the
supporting arm with the holes on the attaching
part. Using the supplied hexagonal wrench (5
mm), secure the supporting arm by tightening the
four hexagonal socket head bolts (M6 x 20 mm).
The standard descriptions on the DS-L2 attaching
part is shown as follows.
• VESA 75 mm standard compliance,
UL1678-compatible
• Attachment screw holes: M4 depth 7 mm
• Tightening torque: 80 to 120N cm
• Leave a clearance of about 100 mm around the
DS-L2 to prevent heat from collecting near the
equipment.
94
Chapter 4
Assembly
■ Attaching the DS-L2
1 Remove the one-touch release attached to the
front of the mount arm for the supporting arm.
Mount arm
attaching/detaching knob
One-touch
release
Pull up the mount arm attaching/detaching knob,
then hold up the docking section forward to
remove it.
Rotation torque
nuts (x2)
2 Detach the stand arm attached on the rear of the
DS-L2 by removing the four clamp screws.
Docking
section
(both sides)
Supporting arm
3 Fix the docking section of the one-touch release to
the rear of the DS-L2 by using the clamp screws
(4) removed in Step 2.
4 Insert the docking section into the upper
one-touch release. Align the pins on the mount
arm with the holes on the docking section to insert
it.
The DS-L2 attached to the supporting arm can be
removed by pulling up the mount arm
attaching/detaching knob.
6 The rotation torque can be adjusted depending on
the tightness of the docking section of the mount
arm to keep the DS-L2 steady, while achieving
more comfortable operation for angle adjustment.
Turn the two nuts on the docking sections with the
supplied tool and control each rotation torque for
the horizontal/vertical direction.
■ Cable connection between the DS-L2 and MA200
(1)
(2)
(3)
1 Connect the DS camera attached to MA200 with
the Camera connector on the left-side of the
DS-L2 using the camera cable exclusive for the
DS camera.
2 Connect the USB (H) connector on the right-side
of the DS-L2 and the [USB] connector on the rear
of the MA200 main body using the USB cable.
3 Connect the AC adapter exclusive for the DS-L2 to
the 12 VDC IN connector on the left side of the
DS-L2.
(2)
95
Chapter 4
4.17
Assembly
Eyelevel Risers
Eyepiece tube
clamp screw
Eyepiece tube
clamp screw
1 Fully loosen the eyepiece tube clamp screw on the
microscope, to mount the eyelevel riser onto the
attaching part for the eyepiece tube, then fitting
the dovetail on the eyelevel riser to the circular
groove of the attaching part.
2 Secure the eyelevel riser by tightening the
eyepiece tube clamp screw.
3 Attach the eyepiece tube on the eyelevel riser and
secure the eyepiece tube with clamp screw.
96
Chapter 4
4.18
Assembly
Camera Adapter
You can perform photomicroscopy by mounting the photomicrographic equipment such as CCD camera on
the microscope with the LV-TV TV or the C-mount adapter attached.
Apply the camera adapter to the vertical tube port on the MA2-TI3 trinocular eyepiece or the back port on the
upper-surface of the microscope. Be sure to apply the adapter or zoom lens compatible with the camera.
Clamp
screw
Camera
1 Remove the cap from the vertical tube port/back
port.
Camera adapter
2 Fully loosen the clamp screw of the side of the
vertical tube port/back port.
Clamp
screw
3 Insert the camera adapter into the hole on the
vertical tube port/back port and secure it by
tightening the clamp screw.
CAUTION
After mounting the camera, be sure to place cables into the cable keeper. If a cable comes into contact
with the lamphouse, the cable sheath may melt resulting in an electrical shock or fire.
4.19
Power Cord
WARNING
Make sure to use the specified power cord. Using a wrong power cord may result in malfunctions or
fire. This product is classified as subject to Class I protection against electrical shock. Make sure it is
connected to an appropriate ground terminal (protective earth terminal).
For specifications of the power cord, refer to “7. Specifications.”
Turn off the power switch on the product (set the switch to the “O” side).
Insert the plug of the power cord into the AC inlet on the back of the product. Then, securely plug the power
cord to the outlet.
97
5
Troubleshooting
Improper use of the microscope may adversely affect performance, even if the microscope is not damaged. If
any of the problems listed in the table below arise, take the countermeasures indicated.
If the problem is not listed below, or if the problem cannot be resolved by the suggested countermeasure,
unplug the power cord and contact your nearest Nikon representative.
5.1
Viewing Problems and Control Problems
Problem
The view-field is invisible,
vignetted, or uneven in brightness.
Epi-fl microscopy
Diascopic microscopy
Cause
Countermeasure
The lamp is not attached correctly.
Install the lamp correctly.
The lamp is not lit.
Be sure that the cable is connected, then turn
the power on.
(p.78)
The optical path changeover lever for the
eyepiece tube/back port selection or the
binocular tube/vertical tube selection is in an
intermediate position.
Securely move the optical path changeover
lever to the position where 100% light goes
through the binocular eyepiece.
(p.33)
A filter or a slider is in an intermediate position.
Move the filter or the slider to a click-stop
position.
(p.40, 43 - 50, 53, 54)
The field diaphragm is stopped down too far.
Open the diaphragm to a suitable size.
The revolving nosepiece is not attached
correctly.
Install the revolving nosepiece correctly. (p.78)
The rotation of the revolving nosepiece is
stopped at an incorrect position. (No objective
is placed in the optical path.)
Turn the revolving nosepiece to the click-stop
position. (Place the objective into the optical
path.)
(p.39)
The sample holder is placed into the optical
path.
Remove it from the optical path.
The BD field changeover lever is in an
intermediate position.
Move the lever to the click-stop position.
The intermediate magnification is in the
intermediate position.
Be sure to switch the position correctly.
No fluorescent unit is attached in place; the
fluorescent unit is attached in a wrong
position.
Attach the fluorescent unit to the correct
position.
(p.50)
The fluorescent unit selection is wrong.
Use a suitable fluorescent unit.
The condenser position is too low.
Adjust the condenser focus knob to enable the
field diaphragm image focus on the sample
surface.
(p.59)
The condenser is not centered.
Center the condenser.
(p.58)
The condenser is not attached correctly.
Install the condenser correctly.
(p.89)
The condenser turret position of the system
condenser is located in an intermediate
position.
Turn it to the click-stop position.
The polarizer is not attached correctly.
Attach the polarizer to the correct position.
(p.93)
98
(p.78)
(p.41)
(p.38)
(p.31)
(p.55)
(p.50)
Chapter 5
Problem
Dirt or dust is seen in the
view-field.
Troubleshooting
Cause
Countermeasure
The aperture diaphragm is stopped down too
far.
Open the diaphragm to a suitable size.
Dirt or dust exists on the intermediate
magnification unit.
Clean the components.
(p.102)
Dirt or dust exists on the lens, eyepiece, filter
or sample.
Clean the components.
(p.102)
Dirt or dust exists on the scale slider or grain
scale slider.
Clean the components.
(p.102)
The upper surface of the condenser is not
clean.
Clean the components.
(p.102)
The condenser position is too low.
Adjust the condenser focus knob to enable the
field diaphragm image focus on the sample
surface.
(p.59)
Dirt or dust exists on the lens, eyepiece, filter,
or sample.
Clean the components.
(p.102)
The current objective is not suitable for the
microscopy.
Use a suitable objective.
(p.32)
Dirt exists on the lens for putting the eyepiece
tube or eyepiece tube entrance lens.
Clean the components.
(p.102)
The aperture diaphragm is stopped down too
far.
Open the diaphragm to a suitable size.
Epi-fl microscopy
The current fluorescent unit is not suited for
the sample.
Use a fluorescent unit suited for the sample.
Diascopic microscopy
The condenser position is too low.
Adjust the condenser focus knob so that the
field diaphragm image is focused on the
sample surface.
(p.59)
The revolving nosepiece is not attached
correctly.
Install the revolving nosepiece correctly.
The revolving nosepiece is not placed to a
click-stop position. (The objective is not placed
in the optical path).
Turn the revolving nosepiece to the click-stop
position.
(p.39)
The sample holder is slanted.
Attach the sample holder correctly.
The revolving nosepiece is not attached
correctly.
Install the revolving nosepiece correctly. (p.78)
The revolving nosepiece is not placed to a
click-stop position.
Turn the revolving nosepiece to the click-stop
position.
(p.39)
The stage is tilting.
Attach the stage correctly.
The microscope is not installed on a flat
surface.
Install the microscope on a flat and level
surface.
The condenser has not been centered.
Center the condenser.
The NCB11 filter is not used.
Place the NCB 11 filter into the optical path.
(p.40)
The lamp voltage is too low.
Increase the brightness with the brightness
control dial, and adjust the brightness with ND
filters.
(p.30, 40)
The lamp voltage is too high.
Adjust the brightness with the brightness
control dial; place an ND filter into the optical
path.
(p.30, 40)
Diascopic microscopy
Viewing is poor (too much/little
contrast or poor resolution).
The focus is uneven.
The image is elongated; the image
shifts during focus.
Diascopic microscopy
The image is tinged yellow.
The image is too bright.
99
(p.42)
(p.42)
(p.50)
(p.78)
(p.80)
(p.80)
(p.58)
Chapter 5
Problem
The brightness is insufficient.
(Refer to the troubleshooting for
the electric system too.)
Troubleshooting
Cause
Countermeasure
The lamp voltage is too low.
Adjust the brightness with the brightness
control dial.
(p.30)
An ND filter is placed in the optical path.
Remove the ND filter from the optical path.
(p.40)
The aperture diaphragm is stopped down too
far.
Open the diaphragm to a suitable size.
A polarizer, analyzer or the fluorescent unit is
placed in the optical path although the
bright-field microscopy is to be performed.
Remove the polarizer, the analyzer, or the
fluorescent unit from the optical path.
A halogen lamp is used for a dark sample.
Replace the light source to the brighter one.
(p.56)
The used objective is not suitable for the
microscopy.
Use the designated objective.
The room is too bright. (for the dark-field
microscopy or the epi-fl microscopy)
Darken the room.
The condenser position is too low.
Adjust the condenser focus knob so that the
field diaphragm image is focused on the
sample surface.
(p.59)
The objective hits the sample
when switched from low to high
magnification. The sample goes
out of focus when switching
objectives.
The eyepiece diopters are not adjusted.
Adjust the diopters.
The eyepieces are not attached correctly.
Mount the eyepieces correctly by aligning the
positioning grooves.
(p.81)
The sample does not move
smoothly.
The sample holder is not secured correctly on
the stage.
Secure the sample holder correctly.
When viewing through the
binocular eyepiece, the image
does not resolve into a single
image.
The interpupillary distance is not adjusted.
Adjust the interpupillary distance.
(p.34)
The eyepiece diopters are not adjusted.
Adjust the diopters.
(p.35)
Eye strain develops while viewing.
The interpupillary distance is not adjusted.
Adjust the interpupillary distance.
(p.34)
The eyepiece diopters are not adjusted.
Adjust the diopters.
(p.35)
The brightness is not appropriate.
Adjust the brightness with the brightness
control dial or ND filters.
(p.30, 40)
Eyepieces with different view-field numbers
are used for the left and right eyes.
Use eyepieces with the same view-field
number.
The coarse focus ring is heavy in
rotation.
The coarse torque adjustment ring is tightened
too much.
Loosen the coarse torque adjustment ring
adequately.
(p.36)
The revolving nosepiece falls on
its own weight and the image goes
out of focus.
The coarse torque adjustment ring is loosened
too much.
Tighten the coarse torque adjustment ring
adequately.
(p.36)
No interference color is seen in
the DIC microscopy.
No polarizer or analyzer is placed in the
optical path.
Place it into the optical path.
The analyzer and the polarizer are not at the
crossed Nicol’s position.
Adjust the orientation of the polarizer to make
the crossed Nicol’s position.
(p.44)
No DIC prism is placed in the optical path.
Place it into the optical path.
The 1/4 λ plate is not attached to the polarizer.
(For Senarmont method)
Place the polarizer equipped with a 1/4 λ plate
into the optical path.
Diascopic microscopy
(p.42)
(p.43, 50)
(p.32)
(p.35)
(p.80)
(p.43)
(p.46, 47)
(p.43)
The combination of the objective and the DIC
prism is wrong.
100
Place an appropriate objective into the optical
path.
(p.46)
Chapter 5
Problem
Troubleshooting
Cause
Uneven colors are seen or low
contrast image is seen in the DIC
microscopy.
Countermeasure
A wrong objective is used.
Place an appropriate objective into the optical
path.
(p.32)
The orientation of the polarizer is wrong.
Adjust the orientation of the polarizer correctly.
(p.44)
No sensitive color is seen in the
DIC microscopy or the polarization
microscopy.
5.2
Dust exists on the objective, the condenser or
the sample.
Clean it. (Pay great attention to dust for the DIC
microscopy.)
(p.102)
The combination of the objective and the DIC
prism is wrong.
Place the DIC prism suitable for the objective
into the optical path.
(p.46)
No λ plate is placed in the optical path.
Place it into the optical path.
(p.45, 49)
Electrical System Problems
Problem
The lamp does not light even
though the power switch is turned
on.
Cause
Countermeasure
No power cord is plugged in. Or, the power
cord is not connected securely.
Connect the power cord correctly.
No lamp is attached.
Attach a lamp.
The lamp is blown.
Replace the lamp with a new one.
(p.97)
(p.78)
(p.78)
The lamp flickers, or its brightness
is unstable.
A wrong lamp is used.
Use the specified lamp.
(Refer to “7. Specifications.”)
The lamp is about to blow.
Replace the lamp with a new one.
The power cord or the cable of the lamphouse
is not connected securely.
Connect the power cord and the lamphouse
cable securely.
(p.77, 97)
The lamp is not securely inserted into the
socket.
Insert the lamp securely.
The lamphouse is not connected securely.
Connect the lamphouse securely.
(p.78)
(p.78)
(p.77)
The brightness control dial does
not function.
Internal/External brightness control
changeover switch is set to ON (external
mode).
Set the Internal/External brightness control
changeover switch to OFF (internal mode).
(p.30)
The address for the objective is
not displayed.
Cable is not connected.
Securely connect the cable.
101
(p.79)
6
Care and Maintenance
Nikon recommends daily care and maintenance for maintaining the performance as long as possible.
Do not let dust, fingerprint, etc., get on the lenses. Dirt on the lenses, filters, and the like will adversely affect
the optical performance of the microscope. If lenses are contaminated, clean them according to the procedure
described in “6.1 Cleaning the Lenses and Filters.” When cleaning, be sure to turn off the power switch (set
the switch to “c” side) to avoid any operation error.
■ Daily care and maintenance
Clean the parts frequently manipulated by hands, such as eyepieces and sample holder, as described in “6.1
Cleaning the Lenses and Filters” without removing them from the microscope. Nikon recommends cleaning
them frequently.
Clean the objectives, filters, and the like to maintain the optical performance. When cleaning the objectives,
remove them from the microscope. Clean them whenever they are contaminated.
Microscopes and stages are contaminated with use. When you find the microscope is contaminated, clean
them according to the description in “6.2 Cleaning the Painted, Plastic, and Printed Parts.”
■ Cleaning tool and supplies (consumables)
• Cleaning tool
Brush (with soft bristles) (Use a cleanroom wiper in a cleanroom.)
• Cleaning supplies (consumables)
Ethyl or methyl alcohol
Lens tissue (Use a cleanroom wiper in a cleanroom.)
6.1
Cleaning the Lenses and Filters
Do not let dust, fingerprint, etc. get on the lenses and filters. Dirt on the lenses, filters, etc. will adversely affect
the view of image. If any lens gets dirty, clean it as described below.
• Either brush away dust with a soft brush, or else gently wipe it off with a piece of gauze.
• Only if there are fingerprints or grease on a lens, dampen lightly a piece of soft, clean cotton cloth, lens
tissue, or gauze with absolute alcohol (ethyl or methyl) and gently wipe off the dirt.
• Absolute alcohol is highly flammable. Be careful when handling it, when around open flames, when turning
the power switch on/off, and so on.
• Follow the instructions provided by the manufacturer when using absolute alcohol.
6.2
Cleaning the Painted Parts, Plastic Parts, and Printed Parts
Do not use organic solvents such as alcohol, ether, or paint thinner on painted components, plastic
components, or printed components. Doing so could result in discoloration or in peeling of the printed
characters. For persistent dirt, dampen a piece of gauze with neutral detergent and wipe gently.
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Chapter 6
6.3
Care and Maintenance
Storage
• Store this product in a dry place where mold is not likely to form.
• Store the objectives and eyepieces in a desiccator or similar container with a drying agent.
• Put the dust-proof cover over this product to protect it from dust.
• Before putting on the dust-proof cover, turn off the power switch of the product (set the switch to the “Ο”
side) and wait until the lamphouse gets cool sufficiently.
6.4
Regular Inspections (fee charged)
Periodical inspections of this product are recommended in order to maintain peak performance. Contact your
nearest Nikon representative for details.
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7
Specifications
Type
ECLIPSE MA200
Dimension
413 (W) × 337 (D) × 308 (H) mm, including the handle and mount parts
Weight
20 kg
Optical system
Objective:
CFI60 system (chromatic aberration free infinity optics system)
Eyepiece:
Field number: 22, 25
Revo. nosepiece:
5 revo. (MA2-NUI5, LV-NU5A)
6 revo. (D-ND6)
7 revo. (D-NI7)
Illumination
Episcopic illumination:
Built-in lamp power supply
Illumination:
Köhler illumination
Microscopy method:
Bright-field, Dark-field, Simplified polarization, Differential interference
contrast and Fluorescence (UV excitation light non-correspondence)
Filter:
ND4, ND16, GIF, and NCB11
Lamp ratings:
12 VDC, 50W halogen lamp
Specified lamp:
LV-HL50W 12V 50W longlife halogen lamp
Specified lamp house:
LV-LH50PC Precentered Lamphouse
Diascopic illumination:
TI-PS100W power supply
Power supply specification
Input ratings:
100-240 VAC (±10%), 1.8 A, 50/60 Hz
Built-in fuse ratings:
250V T4A
Output ratings:
12 VDC 100W
Maximum output current:
8.4 A
Electric shock protection class: Class I
Remarks:
UL listed product, GS approved
Illumination:
Köhler illumination
Microscopy method:
Bright-field, Simplified polarization
Filter:
ND2, ND16, GIF, and NCB11
Lamp ratings:
12 VDC, 100W halogen lamp
Specified lamp:
Halogen lamp (OSRAM HLX64623, PHILIPS77241)
Specified lamp house:
D-LH/LC Precentered Lamphouse LC
Lamphouse attachment method: MA2-DP supporting pillar for dia-Illuminator 100W
Focusing
mechanism
Input ratings
Manual operation type single axis focus knob mechanism
(left side with coarse/fine focus, right side with fine focus knob)
Stroke:
4 mm (±2 mm: top datum surface for stage)
Coarse focus knob:
4.0 mm/revolution (with coarse torque adjustment mechanism)
Fine focus knob:
0.1 mm/revolution (1 μm/marking)
Input voltage:
100-240 VAC
Rated current:
1.2 A maximum
±10% 50/60 Hz
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Chapter 7
Power cord
Specifications
When used in 100-120 V region, outside Japan:
UL listed detachable power cord set, 3 conductor grounding
(3 conductor grounding Type SVT, No.18 AWG, 3 m long maximum, rated at 125 VAC minimum)
When used in 220-240 V region:
Detachable power cord set approved according to EU/EN standard, 3 conductor grounding
(3 conductor grounding Type H05VV-F, 3 m long maximum, rated at 250 VAC minimum)
When used inside Japan:
PSE approved detachable power cord set, 3 conductor grounding
(3 conductor grounding Type VCTF 3x0.75mm2, 3 m long maximum, rated at 125 VAC minimum)
Operation
conditions
Temperature:
0°C to +40°C
Humidity:
85% RH maximum (with no condensation)
Altitude:
2000 m maximum
Pollution degree:
Degree 2
Installation category: Category II
Electric shock
protection class:
Class I
Indoor use only
Transport and
Temperature:
storage conditions Relative humidity:
-20°C to +60°C
Imaging system
Scale:
Slider slot provided
Port:
Eyepiece tube/Back = 100/0
90% RH maximum (with no condensation)
Eyepiece tube/Back = 55/45
Intermediate magnification:
Intermediate magnification unit (MA2-MC) attaching part provided
Applicable
standard
•
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against harmful interference when
the equipment is operated in a commercial environment.
This equipment generates, uses, and can radiate radio frequency energy and, if not installed and
used in accordance with the instruction manual, may cause harmful interference to radio
communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which
case the user will be required to correct the interference at his own expense.
•
This Class A digital apparatus complies with Canadian ICES-003.
Cet appreil numérique de classe A est conforme à la norme NMB-003 du Canada.
•
The product complies with Australian EMI standard. (AS/NZS CISPR11 Group1 Class A)
•
CE marking
•
The product meets EU Low Voltage Directive requirements.
•
The product meets EU EMC Directive requirements. (EN61326)
105