Instructions For Use
Avanti J-E
High-Performance Centrifuge
PN SJ-IM-8AC
December 2013
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Avanti J-E
High-Performance Centrifuge
PN SJ-IM-8AC (December 2013)
© 2002–2013 Beckman Coulter, Inc.
All rights reserved. Made in U.S.A.
All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Beckman Coulter, the stylized logo, and Avanti are
trademarks of Beckman Coulter, Inc. and are registered in
the USPTO.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Find us on the World Wide Web at:
www.beckmancoulter.com
Printed in U.S.A.
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate the instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Warning, Caution, and Note
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety During Installation and/or Maintenance
This instrument is designed to be installed by a Beckman Coulter Field Service representative.
Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty
covering the instrument.
Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is turned off an the
instrument is disconnected from the main power source by removing the Mains (power) plug from
the outlet receptacle, and refer such servicing to qualified personnel.
Use the anchoring system to secure the centrifuge in place. The anchoring system is designed to
reduce the possibility of injury or damage that could result from instrument movement in the event
of a major rotor mishap.
Do not replace any centrifuge components with parts not specified for use on this instrument.
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iii
Safety Notice
Electrical Safety
Electrical Safety
To reduce the risk of electrical shock, this instrument uses a three-wire electrical cord and plug to
connect this equipment to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to
the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the instrument and damage electrical or mechanical components.
Safety Against Risk of Fire
Fuses protect certain electrical circuits within this instrument against overcurrent conditions. For
continued protection against the risk of fire, replace only with the same type and rating specified.
This instrument is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (for example, chloroform or ethyl alcohol) in
this instrument nor handle or store them within the required 30-cm (1-ft area surrounding the
instrument.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only rotors and accessories designed for use in this instrument.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not lift or move the centrifuge while a rotor is spinning.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• In the event of a power failure, do not attempt to retrieve the sample from the centrifuge for at
least 1 hour. Then follow the instructions for sample recovery in CHAPTER 3, Troubleshooting.
iv
PN SJ-IM-8AC
Safety Notice
Chemical and Biological Safety
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument, however, unless all necessary
safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.
•
Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent — Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi — further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before requesting service
by Beckman Coulter Field Service.
RoHS Notice
These labels and materials declaration table (the Table of Hazardous Substance’s Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
China RoHS Caution Label — This label indicates that the electronic information product contains
certain toxic or hazardous substances. The center number is the Environmentally Friendly Use
Period (EFUP) date, and indicates the number of calendar years the product can be in operation.
Upon the expiration of the EFUP, the product must be immediately recycled. The circling arrows
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v
Safety Notice
RoHS Notice
indicate the product is recyclable. The date code on the label or product indicates the date of
manufacture.
China RoHS Environmental Label — This label indicates that the electronic information product does
not contain any toxic or hazardous substances. The center “e” indicates the product is
environmentally safe and does not have an Environmentally Friendly Use Period (EFUP) date.
Therefore, it can safely be used indefinitely. The circling arrows indicate the product is recyclable.
The date code on the label or product indicates the date of manufacture.
vi
PN SJ-IM-8AC
Contents
Safety Notice, iii
Introduction, xi
CHAPTER 1:
Introduction, 1-1
Centrifuge Function and Safety Features, 1-1
Centrifuge Function, 1-1
Safety Features, 1-2
Door, 1-2
Protective Ring, 1-2
Imbalance Detector, 1-3
Overspeed and Rotor Identification System, 1-3
Centrifuge Chassis, 1-3
Housing and Door, 1-3
Rotor Chamber, 1-3
Vacuum, 1-3
Temperature Control, 1-4
Drive, 1-4
Name Rating Plate, 1-4
Controls and Indicators, 1-4
Power Switch, 1-4
Control Panel, 1-4
Digital Displays, 1-5
Run-Parameter Keys, 1-6
Specifications, 1-8
Available Rotors, 1-10
CHAPTER 2:
Introduction, 2-1
Summary of Avanti J-E Run Procedures, 2-1
Programmed Run, 2-2
Manual Run, 2-2
Preparation, 2-3
Installing the Rotor, 2-3
Manual Operation, 2-5
vii
Contents
Selecting a Rotor, 2-5
Entering Run Speed, 2-5
Entering Run Time, 2-6
Entering Run Temperature, 2-6
Entering Acceleration and Deceleration Rates, 2-7
Starting a Run, 2-8
Programmed Operation, 2-8
Creating a New Program, 2-8
Starting a Programmed Run, 2-9
Recalling and Changing a Program, 2-10
Recalling a Program, 2-10
Changing a Program, 2-11
CHAPTER 3:
Introduction, 3-1
User Messages, 3-1
Accessing the Rotor in Case of Power Failure, 3-4
CHAPTER 4:
Introduction, 4-1
Instrument Care, 4-1
Maintenance, 4-1
Cleaning, 4-2
Tube Breakage, 4-3
Decontamination, 4-3
Sterilization and Disinfection, 4-3
Replacing the Air Filter, 4-4
Circuit Breaker and Fuses, 4-5
Storage and Transport, 4-5
Storage, 4-5
Returning a Centrifuge, 4-5
Supply List, 4-5
Replacement Parts, 4-6
Supplies, 4-6
APPENDIX A:
Introduction, A-1
Space Requirements, A-1
Electrical Requirements, A-3
Securing the Centrifuge to the Floor, A-4
Bio-Safety Level 3 Installation, A-4
Beckman Coulter, Inc.
Avanti J-E Centrifuge Warranty
Related Documents
viii
Illustrations
Illustrations
1.1
The Avanti J-E Centrifuge, 1-2
1.2
The Control Panel, 1-5
3.1
Manual Door Release, 3-5
4.1
Air Filter Replacement, 4-4
A.1
Dimensions of the Avant J-E Centrifuge, A-2
A.2
Single-Phase Power Connection, A-3
ix
Tables
Tables
3.1
x
Diagnostic Message Chart, 3-2
Introduction
Certification
Beckman Coulter Avanti J-E centrifuges are manufactured in a facility that maintains certifications
to both ISO 9001:2008 and ISO 13485:2003. They have been designed and tested to be compliant
(when used with Beckman Coulter rotors) with the laboratory equipment requirements of
applicable regulatory agencies. Declarations of conformity and certificates of compliance are
available at www.beckmancoulter.com.
Scope of Manual
This manual is designed to familiarize you with the Avant J-E centrifuge, its functions,
specifications, operation, and routine care and maintenance. Beckman Coulter recommends that
you read this entire manual, especially the Safety Notice and all safety-related information, before
operating the instrument or performing instrument maintenance.
• CHAPTER 1, Description contains system specifications and a brief physical and functional
description of the centrifuge, including the operating controls and indicators, and available
rotors.
• CHAPTER 2, Operation contains centrifuge operating procedures.
• CHAPTER 3, Troubleshooting lists diagnostic messages and other possible malfunctions,
together with probable causes and suggested corrective actions.
• CHAPTER 4, Care and Maintenance contains procedures for routine operator care and
maintenance, as well as a brief list of supplies and replacement parts.
•
APPENDIX A, Preinstallation Requirements provides information about space and power
requirements for installing and connecting the centrifuge.
NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual is the sole responsibility of the user.
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xi
Introduction
Conventions
Conventions
Certain symbols are used in this manual to call out safety related and other important information.
These international symbols may also be displayed on the centrifuge and are reproduced on the
inside back cover of this manual.
Typographic Conventions
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys.
• Key names (for example, START or PROGRAM) appear in bold face type.
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the
Avanti J-E centrifuge.
Recycling Label
A28219-AA
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
1. The device was put on the European market after August 13, 2005 and
2. The device is not to be disposed via the municipal waste collection system of any member state
of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
xii
PN SJ-IM-8AC
CHAPTER 1
Description
Introduction
This chapter provides a brief physical and functional description of the Beckman Coulter Avanti J-E
centrifuge. The operating controls and indicators are also described; instructions for their use are in
CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in
Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.
Centrifuge Function and Safety Features
Centrifuge Function
The Avanti J-E high-performance centrifuge (see Figure 1.1) is a refrigerated centrifuge that
generates centrifugal forces required for a wide variety of applications. Together with the
compatible Beckman Coulter rotors, applications include:
• Routine processing such as sample preparations, pelleting, extractions, purifications,
concentrations, phase separations, and spin column and spin filter centrifugations.
• Rapid sedimentation of protein precipitates, large particles, and cell debris.
• Preparation of subcellular organelles such as mitochondria, nuclei, chloroplasts, and crude
microsomes.
• Separation of blood cells and cellular components.
• Gradient separation, for example, Ficoll-Hypaque and Percoll.
The Avanti J-E is microprocessor-controlled, providing interactive operation. The instrument
design features a brushless switched-reluctance drive motor,* a temperature control system with
automatic temperature compensation unique for each compatible rotor, and selectable
acceleration and deceleration rates.
* Manufactured under license from Switched Reluctance Drives Limited, Harrogate, U.K.
PN SJ-IM-8AC
1-1
Description
Centrifuge Function and Safety Features
Figure 1.1 The Avanti J-E Centrifuge
Manual and programmed operation are available.
• In manual operation, you enter the individual run parameters before beginning each run.
• In programmed operation, you can duplicate runs quickly and accurately by selecting one of
nine programs previously entered into the program memory.
User messages and/or audible signals are provided to alert you to conditions that may need
attention.
Safety Features
The Avanti J-E centrifuge has been designed and tested to operate safely indoors at altitudes up to
2000 m (6562 ft). Safety features include the following.
Door
The steel and structural foam door has an electromechanical door-locking mechanism, with dual
latches, to prevent operator contact with a spinning rotor. When the door is closed and DOOR is
pressed, the latches automatically engage. (Door LED will come on.) It can be unlocked only by
pressing DOOR, and opened only when the power is on and the rotor is at rest. If there is a power
failure, the door lock can be manually tripped for sample recovery (see CHAPTER 3,
Troubleshooting).
Protective Ring
A structural steel ring surrounding the rotor chamber provides full operator protection.
1-2
PN SJ-IM-8AC
Description
Centrifuge Chassis
Imbalance Detector
An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor
loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause an imbalance.
Rotor instability can also occur if the instrument is moved, or if it is not resting level on the floor
(see CHAPTER 3, Troubleshooting).
Overspeed and Rotor Identification System
A rotor identification system prevents the installed rotor from running above its maximum rated
speed. During acceleration the microprocessor checks the magnetic rotor identification. If the
system identifies a rotor different than the one entered by the user, and the set speed is above the
maximum for the identified rotor, the system will reduce the set speed to the maximum for the
installed rotor.
A second check, based on rotor physical properties, is performed during acceleration. Speed is
limited to the maximum safe speed of the identified rotor group.
NOTE Some rotors manufactured before January, 2002, may not have magnets installed. Based on the rotor
property grouping, these rotors may be limited to a lower speed than the rated speed.
Centrifuge Chassis
Housing and Door
The instrument housing is made of steel and molded structural foam. The door and structural-foam
cover panels are finished with polyurethane enamel.
The steel and structural foam door is hinged at the back, providing clearance for loading and
unloading of the centrifuge. In the event of a power failure, the door can be manually unlocked for
sample recovery (see CHAPTER 3, Troubleshooting).
Rotor Chamber
The rotor chamber is made of stainless steel to resist corrosion. A rubber gasket around the
chamber opening ensures sealing. (Instrument gaskets have not been qualified as bioseals for
aerosol containment.)
Vacuum
The friction reduction system (FRS) uses a mechanical rotary vane vacuum pump to reduce
chamber pressure to approximately one-half atmosphere. The pump turns on when the run is
started, before rotor friction reaches a high level. Vacuum in the chamber is vented automatically
during rotor deceleration.
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1-3
1
Description
Name Rating Plate
Temperature Control
The temperature control system cools by circulation of a non-CFC-based refrigerant. The system is
activated when the centrifuge power is on and when the door is closed and latched.
A thermistor in the rotor chamber continuously monitors the chamber temperature. The system
calculates the chamber temperature required to maintain the set rotor temperature, ±2°C.
Although the chamber temperature fluctuates during operation, the rotor’s large mass keeps the
sample temperature substantially constant. At the end of a run, the system continues controlling
the temperature for 30 minutes to prevent freezing or overheating of the sample.
NOTE The system will always shut down using maximum brake if the temperature goes above 50°C.
Drive
The drive shaft is belt driven by a brushless, high-torque, switched-reluctance motor. The
instrument’s resilient suspension minimizes disturbance of the sample during acceleration and
deceleration, and reduces damage to the drive shaft if an imbalance occurs during centrifugation.
Name Rating Plate
A name rating plate is affixed to the rear of the instrument. Always mention the serial number and
model number when contacting Beckman Coulter regarding your Avanti J-E centrifuge.
Controls and Indicators
Power Switch
The power switch, located on the right panel of the centrifuge, controls electrical power to the
centrifuge. It is also a circuit breaker that will trip to cut off power in the event of a power overload.
The power switch must be turned on before the chamber door can be opened.
Control Panel
The control panel (see Figure 1.2) is mounted at an angle on the centrifuge front for easy visibility
and access. It is used to enter run parameters via function keys and a keypad, and to display run
parameters, program information, and user messages.
1-4
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Description
Controls and Indicators
Figure 1.2 The Control Panel
Digital Displays
The displays provide run information, user messages, and diagnostics.
• During a run, they show the actual (real-time) operating conditions.
• When the run parameters are being entered, they show the set values selected. Set values can
be recalled during operation by pressing SHOW SET. After 5 seconds, the displays return to
actual conditions.
PN SJ-IM-8AC
SPEED
Indicates rotor speed in revolutions per minute (RPM), or in relative centrifugal field, a
term describing the ratio of the centrifugal acceleration at a specified radius and speed
to the standard acceleration of gravity (RCF ´ g).
TIME
• In a timed run, indicates the remaining run time in hours and minutes. The time
display begins counting down when START is pressed and continues counting
down to 0, when deceleration begins. The run automatically ends when the set
time reaches zero (a tone sounds when the rotor stops spinning). If a malfunction
shuts down the centrifuge during a run, the time counting stops and the time
remaining when the run stopped remains in the display. By comparing this time
with the original set time, you can determine when the run ended.
• In a hold run, indicates the time elapsed since START was pressed. After 99 hours
and 59 minutes the timer resets to 0 and continues counting elapsed time.
TEMPERATURE
Indicates rotor temperature in degrees C at thermoequilibrium. The displayed
temperature is within ± 2 degrees of the set temperature (after equilibration).
ROTOR
Displays the name of the installed rotor.
ACCEL
When the ACCEL key is pressed, an acceleration rate (1 = MAX, 2 = SLOW) can be
entered. If no number is entered, the instrument accelerates at the rate set for the
previous run.
1-5
1
Description
Controls and Indicators
DECEL
When the DECEL key is pressed, a number representing the selected deceleration rate
(1 = MAX, 2 = SLOW, 3 = OFF) can be entered. If no number is entered, the
instrument decelerates at the rate set for the previous run.
PROGRAM
• During setup — when the PROGRAM key is pressed a program number (one of nine
possible) can be entered.
• During centrifugation — the number of the program being run is displayed
Run-Parameter Keys
Run-parameter keys are used to enter specific run information as follows.
Pressed to display scrolling list of compatible rotors and rotor entry codes. Can be
pressed repeatedly to scroll more quickly through the rotor list.
If the display shows speed in RPM, the key is pressed once to enter run speed (using
the keypad) in RPM (revolutions per minute); pressed twice to enter run speed in RCF
(relative centrifugal field). If the display shows speed in RCF, the key is pressed once to
enter speed in RCF or twice to enter speed in RPM.
Pressed to enter run time using the keypad. When TIME is pressed twice, HOLD mode
is selected. (Pressing it again reverts to time-entry mode.)
• Timed run — Run time up to 99 hours and 59 minutes can be set. If more than
59 minutes are entered in the minutes field, the system automatically converts the
entry to hours and minutes. Deceleration begins when the set time counts down
to zero.
• Continuous run (hold) — For runs of unspecified lengths, hold mode is used. When
99 hours, 59 minutes is reached, the system resets to 0 and continues counting. A
hold run continues until the STOP key is pressed.
Pressed to enter run temperature (using the keypad), from –10 to +40°C. If a new
temperature is not entered, the centrifuge uses the temperature set for the
previous run.
The centrifuge has two acceleration profiles: MAX — maximum acceleration from
0 RPM to set speed, or SLOW — slow acceleration from 0 to 500 RPM, followed by
maximum acceleration to set speed. The ACCEL key is used to select the maximum
acceleration or slow acceleration, provided to maintain optimum separation.
The centrifuge has three deceleration profiles: MAX — full dynamic braking to 0 RPM;
SLOW — brake to 500 RPM followed by reduced braking to a gentle stop; OFF (coast
from set speed to stop). The DECEL key is used to select the deceleration rate provided
to maintain optimum separation.
A toggle switch used to enter into and exit out of the program mode to create or recall
programs. When the key is pressed, the program number can be selected using the
keypad.
Saves the program in memory after program run parameters have been entered.
1-6
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Description
Controls and Indicators
The keypad is used to enter numerical run parameters, to enter or recall a program
number, and to select acceleration and/or deceleration rates. In addition to keys 0
through 9, the keypad includes plus or minus/decimal key and a CE (clear entry) key.
The ± key is used to enter a temperature setting below 0°C (for example, –2°C), or to
enter a decimal point in a rotor entry code, depending on the parameter key selected.
The CE (clear entry) key clears any parameter on the display. Pressing CE will also clear
some diagnostic messages. If a diagnostic message does not clear when CE is pressed,
see CHAPTER 3, Troubleshooting.
The ENTER key can be pressed to save parameter inputs to system memory during
manual run setup, and to start the centrifuge (ENTER then START must be pressed to
start the centrifuge).
Pressed during a run to display set values for 5 seconds.
Pressing ENTER, then START begins the run. (START must be pressed within
5 seconds of pressing ENTER.) This key combination can also be used to abort a
deceleration process and restart the centrifuge.
Can be pressed at any time while the rotor is spinning to terminate a run. The
centrifuge decelerates to a complete stop according to the deceleration setting. The
green light next to the key flashes while the rotor decelerates. An audio indicator
sounds when the rotor comes to a complete stop.
Locks and unlocks the door. (The instrument will not accept the open command if the
rotor is spinning.)
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1-7
1
Description
Specifications
Specifications
Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.
Specification
Description
Speed
• Set speed:
— 300 to 21,000 RPM in 10-RPM increments
• Speed control:
— actual rotor speed, ± 50 RPM of set speed
• Speed display:
— actual rotor speed in 10-RPM increments or actual RCF ´ g
Time
• Set time:
— to 99 hours 59 minutes or continuous HOLD
• Time display
— Timed run: indicates run time remaining
— Hold run: displays elapsed time
Temperature
• Set temperature:
— –10 to +40°C in 1° increments
• Temperature control (after equilibration):
— ±2°C of set temperature
• Ambient temperature range:
— 15 to 35°C
Humidity restrictions
<95% (noncondensing)
Vacuum
1/2 atmosphere
Acceleration
two profiles —MAX (full acceleration to set speed) and SLOW
Deceleration
three profiles — MAX, SLOW, and OFF (coast)
Dimensions
• Width:
— 63.5 cm (25 in.)
• Depth:
— 80.0 cm (31.5 in.)
• Height (door closed):
— 91.4 cm (36 in.)
• Height (door open):
— 152.4 cm (60 in.)
1-8
Weight
267.4 kg (589 lb)
Ventilation clearances
• Sides: 7.6 cm (3.0 in.)
• Rear: 16.0 cm (6.25 in.)
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Description
Specifications
Specification
Description
Finishes
• Control panel:
— silicone elastomer
• Top surface:
— urethane paint
• Other surfaces:
— acrylic baking enamel
Electrical requirements
• 208/240-V instrument:
— 187–229/216–264 VAC, 24 A, 60 Hz
• 230-V instrument:
— 207–253 VAC, 24 A, 50 Hz
• 200-V instrument:
— 180–220 VAC, 24 A, 50 Hz
Electrical supply
Class I
Maximum heat
dissipation into room
under steady-state
conditions (JA-20 rotor)
2 kW (~6900 BTU/h)
Noise level 0.91 m (3 ft)
in front of centrifuge at
21,000 RPM
<64 dBa
Installation
(overvoltage) category
II
Pollution degree
2a
a. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must
be expected.
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1-9
1
Description
Available Rotors
Available Rotors
Refer to the applicable rotor manual for complete rotor descriptions.
Rotor
Profile
1-10
Description
Rotor
Code
Max
RPMa
Max
RCF
(´ g)
Max
Capacity
Rotor
Manual
Number
JA-25.50
Fixed Angle, 34°
(8 place)
rmax = 108 mm
25.5
21,000b
53,300
8 ´ 50 mL
J-TB-056
JA-21
Fixed Angle, 40°
(18 place)
rmax = 102 mm
21
50,400
18 ´ 10 mL
J-TB-002
JA-20.1
Fixed Angle, 23°
(32 place)
rmax = 115 mm
(outer row)
rmax = 98 mm
(inner row)
20.1
32 ´ 15 mL
J-TB-022
JA-20
Fixed Angle, 34°
(8 place)
rmax = 108 mm
20
48,400
8 ´ 50 mL
J-TB-003
JA-18
Fixed Angle, 23°
(10 place)
rmax = 132 mm
18
37,800
10 ´ 100 mL
J-TB-035
JA-14.50
Fixed Angle, 35°
(16 place)
rmax = 160 mm
14.50
14,000e
35,000
16 ´ 50 mL
B32164
JA-17
Fixed Angle, 25°
(6 place)
rmax = 132 mm
17
17,000f
39,800
14 ´ 50 mL
J-TB-017
(18,000
@ 2°C)c
21,000
(18,000
@ 2°C)c
20,000
(17,000
@ 2°C)c
51,500
43,900
20,000
(17,000
@ 2°C)c
16,000d
(14,000
@ 2°C)c
(15,000
@ 2°C)c
PN SJ-IM-8AC
Description
Available Rotors
Rotor
Profile
PN SJ-IM-8AC
Description
Rotor
Code
Max
RPMa
Max
RCF
(´ g)
Max
Capacity
Rotor
Manual
Number
JLA-16.250
Fixed Angle, 25°
(6 place)
rmax = 134 mm
16.25
16,000d
38,400
6 ´ 250 mL
J-TB-072
JA-14
Fixed Angle, 25°
(6 place)
rmax = 137 mm
14
14,000
30,100
6 ´ 250 mL
J-TB-004
F14BCI-14x50cy
Fixed Angle, 34°
(14 place)g
rmax = 153 mm
50
14,000
33,500
14 ´ 50 mL
—
F14BCI-6x250y
Fixed Angle, 23°
(6 place)
rmax = 137 mm
250
14,000
30,000
6 ´ 250 mL
—
JA-12
Fixed Angle, 35°
(12 place)
rmax = 144 mm
12
12,000
23,200
12 ´ 50 mL
J-TB-051
JA-10
Fixed Angle, 25°
(6 place)
rmax = 158 mm
10
10,000h
17,700
6 ´ 500 mL
J-TB-006
JLA-10.500
Fixed Angle, 20°
(6 place)
rmax = 166 mm
10.5
10,000h
18,600
6 ´ 500 mL
J-TB-048
F10BCI-6x500y
Fixed Angle, 23°
(6 place)
rmax = 158 mm
500
10,000
17,696
6 ´ 500 mL
—
JLA-9.1000
Fixed Angle, 20°
(4 place)
rmax = 185 mm
9.1
6300 b
8230
4 ´ 1000 mL
J-TB-073
(14,000
@ 2°C)c
1-11
1
Description
Available Rotors
Rotor
Profile
Description
Rotor
Code
Max
RPMa
Max
RCF
(´ g)
Max
Capacity
Rotor
Manual
Number
JS-13.1
Swinging Bucket
(6 place)
rmax = 140 mm
13
13,000
26,500
6 ´ 50 mL
J-TB-036
JS-5.3
Swinging Bucket
(4 place)
rmax = 194.8 mm
5.3
6130
4 ´ 500 mL
24 microplates
8 deep-well plates
4 square-well
plates
J-TB-089
(12,000
@ 2°C)c
5300
a. Maximum speeds are based on a solution density of 1.2 g/mL in all rotors.
b. Maximum speed for the rotor in the Avanti J-E centrifuge.
c. At 35°C ambient temperature and 95 percent humidity.
d. Maximum speed in the Avanti J-E for the rotor with magnets; without magnets maximum is 14,000 RPM. (Maximum speed
at 2°C in a 50-Hz centrifuge is 14,000 RPM.)
e. Temperature performance for the JA-14.50 rotor is as follows: 4°C minimum at 14,000 RPM (at 35°C ambient).
f. Maximum speed for rotor without magnets is 13,000 RPM.
g. Temperature performance for the F14BCI-14x50cy rotor in the Avanti J-E is as follows: 12°C minimum at 14,000 RPM (at 35°C
ambient); 4°C minimum at 13,000 RPM (35°C ambient).
h. Maximum speed for rotor without magnets is 6300 RPM.
1-12
PN SJ-IM-8AC
CHAPTER 2
Operation
Introduction
This section contains manual and programmed operating procedures. A summary is provided at the start
of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick
review of operating steps.
WARNING
Normal operation may involve the use of solutions and test samples that are
pathogenic, toxic, or radioactive. Handle body fluids with care because they can
transmit disease. No known test offers complete assurance that they are free of
micro-organisms. Some of the most virulent — Hepatitis (B and C) and HIV (I–V)
viruses, atypical mycobacteria, and certain systemic fungi — further emphasize
the need for aerosol protection. Handle other infectious samples according to
good laboratory procedures and methods to prevent spread of disease. Because
spills may generate aerosols, observe proper precautions for aerosol
containment.
Do not run toxic, pathogenic, or radioactive materials in this centrifuge without
taking appropriate safety precautions. Biosafe containment should be used when
Risk Group II materials (as identified in the World Health Organization Laboratory
Biosafety Manual) are handled; materials of a higher group require more than one
level of protection.
WARNING
Do not use the centrifuge in the vicinity of flammable liquids or vapors, and do not
run such materials in the centrifuge. Do not lean on the centrifuge or place items
on it while it is operating.
Summary of Avanti J-E Run Procedures
For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.
PN SJ-IM-8AC
2-1
Operation
Summary of Avanti J-E Run Procedures
Programmed Run
1
Turn the POWER switch on (I).
2
Press DOOR to unlock the chamber door; lift the door open.
3
4
5
6
7
Install the rotor according to the applicable rotor manual, then close the chamber door and
press DOOR.
Press PROGRAM, use the keypad to enter the required program number, then press ENTER. Press
SHOW SET to display program parameters.
Check that all parameters are correct and that the door is closed. Press ENTER, then press START
(within 5 seconds).
Wait for the run to end, or end the run by pressing STOP.
When the rotor stops (a tone sounds), press DOOR to unlock the chamber door; lift the door
open to remove the rotor.
Manual Run
1
Turn the POWER switch on (i).
2
Press DOOR to unlock the chamber door; lift the door open.
3
4
5
2-2
Install the rotor according to the applicable rotor manual, then close the chamber door and
press DOOR.
Press ROTOR, then use the keypad to enter the rotor entry code.
Press RPM/RCF, then use the keypad to enter the run speed (300 to 21 000 rpm). Press RPM/RCF
a second time to enter speed in RCF.
PN SJ-IM-8AC
Operation
Preparation
6
Press TIME, then use the keypad to enter the run time (to 99 hrs, 59 minutes); or press TIME
twice for a hold (continuous) run.
7
Press TEMP°C, then use the keypad to enter the required run temperature (–10 to +40°C).
8
Press ACCEL, then use the keypad to enter the selected acceleration rate number, 1 or 2.
9
Press DECEL then use the keypad to enter the selected deceleration rate number, 1, 2, or 3.
10 Check that all parameters are correct and that the door is closed. Press ENTER then press START
(within 5 seconds).
11 Wait for the run to end, or end the run by pressing STOP.
12 When the rotor stops (a tone sounds), press DOOR to unlock the chamber door; lift the door
open to remove the rotor.
Preparation
Prepare the rotor for centrifugation as described in the applicable rotor manual. For runs at other
than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.
Installing the Rotor
The power must be turned on before you can unlock and open the chamber door. (To end a run for
any reason, do not turn the power switch off; press STOP instead.)
1
Turn the power switch on (I).
Indicator lights on the control panel light up.
2
Press DOOR to unlock the door.
The instrument will accept this command only when the rotor is at rest.
3
PN SJ-IM-8AC
Lift the door up to open.
2-3
2
Operation
Preparation
4
Install the rotor according to directions in the rotor manual.
a.
Ensure that the rotor is seated on the drive hub.
b. Securely attach the rotor lid knob, or tie-down knob in rotors without lids, to the drive
shaft by turning it to the right (clockwise).
NOTE If the knob turns loosely and you do not feel threads engaging, the rotor drive hole pins may
not be properly seated on the centrifuge hub. Lift the rotor up above the hub, rotate it slightly,
and lower it onto the hub again. Tighten the knob.
c. Avoid bumping the control panel keys during rotor installation or removal.
d. If you bump the control panel and an L1 diagnostic appears, press CE to clear the message.
CAUTION
Rotors used in Avanti J series centrifuges must have drive pins in the rotor drive
hole. These drive pins engage with the centrifuge drive hub to ensure that the
rotor does not slip during acceleration. Some Beckman Coulter rotors were
originally manufactured without drive pins because these rotors did not need pins
to run in older model centrifuges. To check for drive pins, hold the rotor up or turn
it on its side and look into the drive hole. If you do not see at least two metal pins
near the top of the hole, do not use the rotor in the Avanti J-E.
1. Drive Pins
(Angled pins shown.
Pins can also be vertical
or horizontal.)
2. Drive Spindle Assembly
1
2
5
Close the chamber door and press DOOR.
The door latches.
When the latches engage, the LED next to the DOOR key lights.
To keep the chamber clean and dry, leave the door closed whenever possible.
2-4
PN SJ-IM-8AC
Operation
Manual Operation
Manual Operation
When a run-parameter key (ROTOR, RPM/RCF, TIME, TEMP, ACCEL, or DECEL) is pressed, a parameter
can be entered or changed. Entry is completed when ENTER or another function key is pressed.
1
To change an entry before you’ve pressed ENTER or another parameter key, press CE and enter
a different value.
a. To change an entry after you’ve pressed ENTER, press the run-parameter key again.
If an unacceptable value is entered, the valid range for that parameter is displayed in the
message line.
b. Enter the correct value.
Selecting a Rotor
1
Press ROTOR
A list of rotors, with rotor entry codes, scrolls across the display.
2
Use the keypad to select a rotor by entry code number.
a. An alternate method of selecting a rotor is to press ROTOR repeatedly.
The rotor number changes each time the key is pressed.
b. When the rotor you are using is displayed, press ENTER.
3
Press the next run-parameter key or press ENTER.
Entering Run Speed
Enter a run speed up to the maximum speed of the rotor in use (21,000 RPM maximum). Or, enter a
relative centrifugal field (RCF) value up to the maximum achievable RCF of the rotor.
1
Press RPM/RCF once to enter speed in RPM or twice to enter speed in RCF.
Allowed speed for the selected rotor is displayed.
2
Use the keypad to enter required speed.
Entered speed appears on the speed display.
PN SJ-IM-8AC
2-5
2
Operation
Manual Operation
If the entered speed is higher than the rated speed of the installed rotor, a message showing the
valid speed range will be displayed.
a. Press CE and enter an acceptable value.
3
Press the next run-parameter key or ENTER.
You can change the set speed at any time by repeating steps 1 through 3.
The rotor will accelerate or decelerate to the new speed.
NOTE During acceleration, the instrument identifies the rotor and checks its maximum allowable speed.
If set speed is greater than the rotor group’s maximum allowable speed, the rotor will decelerate to
its rated speed, and an error message will be displayed. Refer to CHAPTER 3, Troubleshooting.
Entering Run Time
Run time can be set for up to 99 hours and 59 minutes or for a hold (continuous) run. The time
display begins counting down when the rotor starts to spin. For a timed run, the run automatically
terminates when the set time reaches zero; a tone sounds when the rotor has stopped. For a hold
run, the elapsed time is displayed and the run continues until STOP is pressed.
1
Press TIME.
The cursor appears.
2
Use the keypad to enter required time.
Entered time appears on the display.
If you entered a number higher than 59 minutes, the centrifuge automatically recalculates the
time in hours and minutes.
a. Or, press TIME again for a hold run.
HOLD appears on the display.
3
Press the next run-parameter key or ENTER.
Entering Run Temperature
Run temperature can be set from –10 to +40°C. If no value is entered, the centrifuge selects the last
entered temperature.
1
Press TEMP.
The cursor and the temperature range for the selected rotor appear.
2-6
PN SJ-IM-8AC
Operation
Manual Operation
2
Use the keypad to enter required temperature.
Entered temperature appears on the display.
(If the entered temperature is lower than can be achieved for the installed rotor, a message
showing “Input error temp” will be displayed.)
3
Press the next run-parameter key or ENTER.
Entering Acceleration and Deceleration Rates
The instrument provides a choice of two acceleration rates and three deceleration rates to protect
the gradient and sample-to-gradient interface. The acceleration time is the time it takes a rotor to
reach set speed from rest. The deceleration time is the time it takes a rotor to decelerate from set
speed to rest.
1
Press ACCEL.
Acceleration options are displayed.
2
Press the keypad number for the required acceleration rate — 1 = MAX, 2 = SLOW.
Entered acceleration rate appears on the display.
3
Press the next run-parameter key or ENTER.
4
Press DECEL.
Deceleration options are displayed.
5
Press the keypad number for the required deceleration rate — 1 = MAX; 2 = SLOW; 3 = OFF.
Entered deceleration rate appears on the display.
PN SJ-IM-8AC
2-7
2
Operation
Programmed Operation
Starting a Run
1
Press ENTER and START.
The green light next to the START key flashes and the rotor begins to spin.
NOTE To begin a run, ENTER must always be the last key pressed before pressing START. If you wait
more than 5 seconds, the START key will not activate. If this happens, press ENTER and START
again to begin the run.
If an unacceptable value was entered, the run will not begin when ENTER and START are
pressed.
a. Check the display and make any necessary corrections or additions.
b. Press ENTER and START again to begin the run.
The run will end when the time display counts down to zero.
c. Press STOP to terminate a run for any reason.
The green light next to the STOP key will flash while the rotor decelerates.
A tone sounds when the rotor comes to a complete stop.
2
After the rotor has stopped, press DOOR.
The door is unlocked.
3
Remove the rotor.
Keep the chamber door closed between runs.
Programmed Operation
The instrument internal memory can store up to nine programs which can be recalled by keypad
numbers 1 through 9. Saved programs are retained in memory even if the power is turned off.
Procedures for entering run parameters are the same for programmed operation as those described
above for manual operation.
Creating a New Program
1
Press PROGRAM.
Previously saved program numbers are displayed.
2-8
PN SJ-IM-8AC
Operation
Programmed Operation
2
Use the keypad to select a program number that is not in use or one that you want to overwrite
and press ENTER.
The message “Press ENTER START to run, or edit parameters” is displayed.
3
Enter run parameters (speed, time, temperature, acceleration/deceleration rate settings).
If you entered an unacceptable parameter, an error message will be displayed.
a. Press CE and enter an acceptable value, then press the next run-parameter key.
4
When all parameters are entered, press SAVE.
The program is saved into memory.
NOTE Record the program parameters on the Program Library record (233679).
5
Press ENTER START to run the programmed run (or press PROGRAM to exit program mode and
return to manual operation).
Starting a Programmed Run
1
Press PROGRAM.
Previously saved program numbers are displayed.
2
Use the keypad to enter the number of the program, then press ENTER.
The message “Press ENTER START to run, or edit parameters” is displayed.
3
Press SHOW SET.
The number you selected will appear in the program display.
Run parameters for that program will be displayed for 5 seconds.
(To make changes, see Changing a Program, below.)
4
Press ENTER and START to begin the programmed run (or press PROGRAM to exit program mode
and return to manual operation).
The run starts, the green light next to the START key flashes, and the rotor begins to spin.
NOTE To begin a run, ENTER must always be the last key pressed before pressing START. If you wait
more than 5 seconds, the START key will not activate. If this happens, press ENTER and START again
to begin the run.
PN SJ-IM-8AC
2-9
2
Operation
Programmed Operation
If an unacceptable value was entered, the run will not begin when ENTER and START are
pressed.
a. Check each display and make any necessary corrections or additions.
b. Press ENTER and START again to begin the run.
The run will end automatically when the time display counts down to zero.
c. Press STOP to terminate a run at any time.
The green light next to the STOP key will flash while the rotor decelerates.
A tone will sound when the rotor has come to a complete stop.
5
After the rotor has stopped, press DOOR.
The door is unlocked.
6
Remove the rotor. Keep the chamber door closed between runs.
Recalling and Changing a Program
Recalling a Program
A program that has been saved in memory can be recalled at any time.
1
Press PROGRAM.
Previously saved program numbers in use are displayed.
2
Select the appropriate keypad number and press ENTER.
The message “Press ENTER START to run, or edit parameters” is displayed.
3
Press SHOW SET to display program parameters.
4
To begin the run, press ENTER and START.
The run starts.
2-10
PN SJ-IM-8AC
Operation
Programmed Operation
Changing a Program
1
Press PROGRAM.
Previously saved program numbers are displayed.
2
Select the appropriate keypad number and press ENTER.
The message “Press ENTER START to run, or edit parameters” is displayed.
3
Press SHOW SET to display program parameters.
4
Press the run parameter key to be changed.
Parameter prompts are displayed.
5
Use the keypad to enter the new value.
6
Repeat steps 4 and 5 to change other run parameters.
a. When all changes have been entered, press SAVE.
The revised program will remain in memory until further changes are made and saved.
b. Record the changes on your Program Library record (233679).
7
Press ENTER and START to begin the programmed run (or press PROGRAM to exit program mode
and return to manual operation).
The run starts.
PN SJ-IM-8AC
2-11
2
Operation
Programmed Operation
2-12
PN SJ-IM-8AC
CHAPTER 3
Troubleshooting
Introduction
This section lists possible malfunctions, along with probable causes and corrective actions. Maintenance
procedures are given in CHAPTER 4, Care and Maintenance. For any problems not covered here, call
Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada) or contact your local
Beckman Coulter Representative for assistance.
NOTE It is your responsibility to decontaminate the instrument, as well as any rotors and/or accessories,
before requesting service by Beckman Coulter Field Service.
User Messages
User messages appear on the setup screen to communicate information about the instrument or to
alert you to abnormal conditions that need attention.
• Help and informational messages caused by incorrect input or certain operating conditions can
be cleared by pressing CE and following the instructions in the message.
• Diagnostic messages that result from abnormal operating conditions or equipment
malfunction require troubleshooting. Refer to Table 3.1 to determine the nature of the
condition and any recommended actions. If a problem persists after you have performed the
recommended action, call your Beckman Coulter Field Service Representative at
1-800-742-2345 (U.S.A. or Canada) or contact your local Beckman Coulter office. To help the
field service representative diagnose and correct the problem, try to gather as much
information about the situation as you can, including:
— the diagnostic number and message,
— the operating situation when the diagnostic condition occurred (such as rotor in use,
speed, or load type), and
— any unusual environmental and/or operating conditions (such as ambient temperature or
voltage fluctuations).
NOTE The information provided in Table 3.1 is a user guide, and is not a comprehensive checklist.
PN SJ-IM-8AC
3-1
Troubleshooting
User Messages
Table 3.1 Diagnostic Message Chart
Diagnostic Message
Problem
Result
Recommendation
P1–
Power failure
occurred, see manual
Momentary power failure:
rotor does not come to a
complete stop
Run continues when
power resumes
Press CE to clear message.
P2–
Power failure, see
manual
Power failure: rotor speed
drops to <500 rpm
Run restarts
automatically when
power resumes
Press CE to clear message.
L1, L2, L5, L6, L11,
and L12–
Reclose door
Latches are not operating
properly
Error message
appears; run shuts
down with maximum
brake
Press down on the door and press DOOR. If
you close the door repeatedly and the
problem continues, gently clean the latch
area with a lintless swab. Be careful not to
damage sensitive electronics in the area.
WARNING: Do not put your fingers into the
latch openings.
Press CE to clear message.
C1–
Rotor temp exceeds
4C above set
Rotor temperature
exceeds temperature
setting by more than 4°C
but less than 8°C
Run continues
Press CE to clear message.
C2–
Rotor temp exceeds
8C above set
Rotor temperature
exceeds temperature
setting by more than 8°C
Run shuts down with
maximum brake
Call Beckman Coulter Customer Servicea.
C3–
Temp, call service
Cannot maintain
temperature
Run shuts down with
maximum brake
• Check the air filter and replace if dirty
(see CHAPTER 4, Care and Maintenance).
• Call Beckman Coulter Customer Service.
C5–
Temp, call service
Refrigeration system error Run shuts down with
maximum brake
Call Beckman Coulter Customer Service.
T1 through T4–
Temp, call service
System temperature
problem
Run shuts down with
maximum brake
Call Beckman Coulter Customer Service.
Run stops, usually with
no brake. Door
may not unlock for up
to an hour.
Call Beckman Coulter Customer Service.
Before trying to open the door, listen
carefully and make sure that no sound is
coming from the chamber (indicating a
spinning rotor). Follow the directions under
Accessing the Rotor in Case of Power Failure,
below.
D1 through D12, D14, Drive system problem
and D15–
Drive, call service
D13–
There is no rotor installed Run shuts down with
No rotor in chamber or or the drive belt is loose or maximum brake.
broken
drive problem
3-2
• Install rotor per the applicable rotor
manual.
• If the rotor is installed when the message
appears, call Beckman Coulter Customer
Service.
PN SJ-IM-8AC
Troubleshooting
User Messages
Table 3.1 Diagnostic Message Chart (Continued)
Diagnostic Message
Problem
Fl and F2–
FRS,b call service
Required vacuum level not Run shuts down with
reached in allowed time
maximum brake
• Check and clean door sealing area and
door gasket.
• Wipe any ice and excess moisture from
chamber.
• Call Beckman Coulter Customer Service.
R1, and R2–
Rotor, ID problem
No magnets identified, or
magnets incorrectly
identified
Run continues, speed
may be derated
Press CE to clear message. If problem
repeats, check rotor magnets or call
Beckman Coulter Customer Service.
R3, R4, and R8–
Rotor, speed derated
The entered rotor number
is not the same as the
rotor identified
The entered rotor
number is not the
same as the rotor
identified
Press CE to clear message. Enter the correct
rotor entry code.
R5 and R6–
No rotor match
The system cannot
identify the rotor
Run shuts down with
maximum brake
• Make sure the rotor in use is a compatible
Beckman Coulter rotor (see Available
Rotors in CHAPTER 1).
• Call Beckman Coulter Customer Service.
R9–
Calibration error
Rotor calibration error
Run shuts down with
maximum brake
• Make sure the rotor in use is a compatible
Beckman Coulter rotor (see Available
Rotorsin CHAPTER 1).
• Call Beckman Coulter Customer Service.
S1 through S14System error, call
service
There is a problem with
the system control
software, EPROM, or RAM
System shuts down
Call Beckman Coulter service
H1, H5, H7, and H8Speed, call service
Speed control problem
Run shuts down with
maximum brake, door
may not unlock for up
to an hour.
Call Beckman Coulter service
H2, H3, and H11Speed, call service
Speed control problem
Run stops, usually with Call Beckman Coulter service
no brake
H4, H6, and H9Speed error
Accel or decel speed
problem
Run continues
PN SJ-IM-8AC
Result
Recommendation
Press CE to clear message
3-3
3
Troubleshooting
Accessing the Rotor in Case of Power Failure
Table 3.1 Diagnostic Message Chart (Continued)
Diagnostic Message
Problem
Result
Recommendation
l1Rotor imbalance
Rotor load is severely out
of balance
Run shuts sown with
maximum brake
• Make sure that tubes or bottles are
loaded symmetrically in the rotor.
• With swinging bucket rotors, remove the
buckets and lubricate the pivot pins
where the buckets contact as described
in the applicable rotor manual.
Unlubricated pivot pins can prevent the
buckets from reaching horizontal
position, which can cause imbalance.
—
During low-temperature
runs (near -10°C), ice
forms around the door
opening
Door will not open at
the end of a run
To minimize icing, wipe moisture from the
chamber, the chamber gasket and the inner
door surface before each run. Keep the door
closed as much as possible.
a. For Beckman Coulter Customer Service call 1-800-742-2345 (U.S.A. or Canada) or contact your local Beckman Coulter office.
b. Friction Reduction System.
Accessing the Rotor in Case of Power Failure
If facility power fails only momentarily, the instrument will resume operation when power is
restored and the rotor will return to set speed. However, if the rotor came to a complete stop, you
will have to restart the run when the power is restored. In either case, a power outage message
will be displayed on the control panel to indicate that a power outage has occurred.
WARNING
Any maintenance procedure requiring removal of a panel exposes the operator to
the possibility of electrical shock and/or mechanical injury. Therefore, turn the
power off (O) and disconnect the instrument from the main power source by
removing the Mains (power) plug from the outlet receptacle, and refer such
maintenance to qualified service personnel.
In the event of an extended power failure, it may be necessary to override the door-locking
mechanism manually to remove the rotor and retrieve your sample.
WARNING
The following procedure should be implemented only when absolutely necessary
and only by qualified service personnel.
1
Turn the power off and disconnect the power cord from the main power source.
No lights on the control panel are lit.
3-4
PN SJ-IM-8AC
Troubleshooting
Accessing the Rotor in Case of Power Failure
2
Insert the hex wrench (729317) or a similar tool into the openings on each side of the centrifuge
(see Figure 3.1) to release the top front panel.
CAUTION
LISTEN CAREFULY. Do not proceed if any sound or vibration is coming from
the drive.
Figure 3.1 Manual Door Release
1
2
TM
1
1. Hex Wrench
3
2. Latch Override Key
Lower the panel as far as the tether cords allow.
CAUTION
Do not attempt to remove tether cords. They are required to assure safe operation
of the centrifuge.
4
Use the latch override key (368247) to turn the right-hand latch bolt to the right (clockwise) and
the left-hand latch bolt to the left (counterclockwise) until the latches release.
If the rotor is still spinning, close the door and turn the latches to secure it.
Wait until the rotor stops spinning to repeat this step.
PN SJ-IM-8AC
3-5
3
Troubleshooting
Accessing the Rotor in Case of Power Failure
5
After removing the rotor, replace the top front panel by pushing it into place.
WARNING
NEVER try to slow or stop the rotor by hand.
3-6
PN SJ-IM-8AC
CHAPTER 4
Care and Maintenance
Introduction
This section describes routine care and maintenance procedures that you should perform regularly. For
maintenance not covered in this manual, call Beckman Customer Service at 1-800-742-2345 (U.S.A. or
Canada) or contact your local Beckman Coulter office for assistance. User messages and recommended
actions are discussed in CHAPTER 3, Troubleshooting. Refer to the applicable rotor manual and Rotors
and Tubes for J Series Centrifuges (publication JR-IM) for instructions on the care of rotors and their
accessories.
Instrument Care
NOTE It is your responsibility to decontaminate the instrument, as well as any rotors and/or accessories,
before requesting service by Beckman Coulter Field Service.
WARNING
Any maintenance procedure requiring removal of a panel exposes the operator to
the possibility of electrical shock and/or mechanical injury. Therefore, turn the
power off and disconnect the instrument from the main power source, and refer
such maintenance to service personnel.
Maintenance
Perform the following procedures regularly to ensure continued performance and long service life
of the centrifuge.
1
Inspect the centrifuge chamber for accumulations of sample, dust, or glass particles from
broken sample tubes.
Clean as required (see Cleaning below).
PN SJ-IM-8AC
4-1
Care and Maintenance
Maintenance
2
Check the air filter on the back panel for obstructions.
Keep vents clear and clean.
3
4
Wipe condensation out of the chamber between runs with a sponge or clean cloth to prevent
chamber icing.
If chamber icing occurs, defrost the system and wipe moisture out of the chamber before use.
To defrost the system, set the temperature to 30°C for 20 minutes.
(These are suggested settings that may be adjusted as appropriate for your laboratory
conditions.)
NOTE Before using any cleaning or decontamination methods except those recommended by the
manufacturer, users should check with the manufacturer that the proposed method will not damage the
equipment.
Cleaning
Clean the centrifuge frequently. Always clean up spills when they occur to prevent corrosives or
contaminants from drying on component surfaces.
1
To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes,
keep the chamber clean and dry by frequent wiping with a cloth or paper towel.
For thorough cleaning, wash the chamber using a mild detergent such as Solution 555.
Dilute the detergent with water (10 parts water to 1 part detergent).
Rinse thoroughly and dry completely.
2
Clean the centrifuge exterior surfaces by wiping with a cloth dampened with Solution 555.
Dilute the detergent with water (10 parts water to 1 part detergent).
Do not use acetone.
3
Clean the drive hub regularly using Solution 555 and a soft brush.
Dilute the detergent with water (10 parts water to 1 part detergent).
Rinse thoroughly and dry completely.
4-2
PN SJ-IM-8AC
Care and Maintenance
Maintenance
Tube Breakage
If a glass tube breaks, and all the glass is not contained in the bucket or rotor, be sure to thoroughly
clean the chamber.
WARNING
Be careful when examining or cleaning the chamber and chamber gasket, as sharp
glass fragments may be embedded in their surfaces.
1
Examine the chamber gasket to make sure that no glass particles are retained in it.
Carefully remove any glass particles that may remain.
2
Carefully wipe away any glass particles that remain in the chamber.
Decontamination
If the instrument and/or accessories are contaminated with radioactive or pathogenic solutions,
perform all appropriate safety and decontamination procedures. Refer to Chemical Resistances
(publication IN-175) to be sure the decontamination method will not damage any part of the
instrument
Sterilization and Disinfection
The centrifuge is finished with urethane paint. Ethanol (70%) may be used on this surface. See
Chemical Resistances for chemical compatibilities of centrifuge and accessory materials.
CAUTION
Ethanol is a flammability hazard. Do not use it in or near operating centrifuges.
While Beckman Coulter has tested ethanol (70%) and found that it does not damage the centrifuge,
no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection
is a concern, consult your laboratory safety officer regarding proper methods to use.
PN SJ-IM-8AC
4-3
4
Care and Maintenance
Maintenance
Replacing the Air Filter
1
Check the air filter (see Figure 4.1) regularly and replace it about once a year, or more often if
it looks dirty.
The air filter is not fastened to the centrifuge, so no tools are required for removal or
installation.
2
3
To remove the air filter, grasp the top edge and lift the filter straight up.
Install a new filter by holding the top edge and inserting the bottom edge into the slot in the air
deflector strip.
Lower the filter until the bottom edge rests on the brackets.
Figure 4.1 Air Filter Replacement
1
3
2
1. Filter
2. Filter Brackets
3. Air Deflector
4-4
PN SJ-IM-8AC
Care and Maintenance
Circuit Breaker and Fuses
Circuit Breaker and Fuses
There are no user-replaceable fuses in the Avanti J-E centrifuge.
If the centrifuge circuit breaker trips for any reason, the power switch will move to the OFF (O)
position. Reset the circuit breaker by turning the power switch back to the ON (I) position. If it trips
again immediately, do not reset it. Call Beckman Coulter Field Service.
CAUTION
Repeated attempts to reset the centrifuge circuit breaker can cause substantial
damage to electrical and electronic components.
Storage and Transport
Storage
To ensure that the centrifuge does not get damaged, contact Beckman Coulter Field Service for
specific instructions and/or assistance in preparing the equipment for transport or long-term
storage. Temperature and humidity conditions for storage should meet the environmental
requirements described under Specifications in CHAPTER 1.
Returning a Centrifuge
Before returning a centrifuge or accessory for any reason, prior permission (a return authorization
form) must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to
obtain the return form and instructions for packaging and shipping.
To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from
pathogens and/or radioactivity. Sterilization and decontamination must be done before returning
the parts.
All parts must be accompanied by a note, plainly visible on the outside of the box or bag, stating that they
are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach
this notification will result in return or disposal of the items without review of the reported problem.
Supply List
Call Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada) or visit
www.beckman.com for information about ordering parts, supplies, and publications. For your
convenience, a partial list is given below. See the Beckman Coulter High Performance, High Speed,
High Capacity Rotors, Tubes & Accessories catalog (BR-8102, available at www.beckmancoulter.com)
for detailed information on ordering rotors, tubes, and accessories.
PN SJ-IM-8AC
4-5
4
Care and Maintenance
Supply List
Replacement Parts
Description
Part Number
Air filter
978347
Latch override key
368247
Memory flash card
369173
Supplies
NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.
4-6
Description
Part Number
Silicone vacuum grease (1 oz)
335148
Solution 555 (1 qt)
339555
Hex key (panel removal
729317
Program library record
233679
PN SJ-IM-8AC
APPENDIX A
Preinstallation Requirements
Introduction
Preinstallation requirements have been provided for your Avanti J-E centrifuge. The following information
is included in case the instrument must be relocated. (After the centrifuge is moved it must be leveled by
adjusting the two front feet.)
NOTE This centrifuge is designed to be installed by a Beckman Coulter Field Service representative.
Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty
covering the instrument.
Space Requirements
WARNING
Do not place the centrifuge near areas containing flammable reagents or
combustible fluids. Vapors from these materials could enter the centrifuge air
system and be ignited by the motor. Maintain a 30-cm (1-ft) clearance area around
the centrifuge while it is running. No persons or any hazardous materials
should be within this area while the centrifuge is operating except to change
operating controls, if required.
If it is necessary to move the centrifuge, maintain the following conditions.
1
Select a location away from heat-producing laboratory equipment, with sufficient ventilation
to allow for heat dissipation.
The centrifuge must have adequate air ventilation to ensure compliance to local requirements
for vapors produced during centrifuge operation.
2
PN SJ-IM-8AC
Position the centrifuge on a level floor that can support the weight of the centrifuge and resist
vibration.
A-1
Preinstallation Requirements
Space Requirements
3
4
In addition to space for the instrument itself, allow a 7.7-cm (3-in.) clearance on each side of the
instrument and a 16-cm (6.25-in.) clearance behind the instrument for air circulation.
Position the centrifuge so that the air diverter nearly touches the wall behind the centrifuge
(see Figure A.1).
a. Place the power cord to one side of the air diverter.
b. To avoid damaging the power cord when installing or moving the centrifuge, be sure to
move the cord out of the way before pushing the centrifuge toward the wall.
Relative humidity should not exceed 95% (noncondensing).
Figure A.1 Dimensions of the Avant J-E Centrifuge
in.
cm
60.0
152.4
34.0
86.4
6.25
16.0
3.0
7.7
31.5
80.0
25.0
63.5
3.0
7.7
A-2
PN SJ-IM-8AC
Preinstallation Requirements
Electrical Requirements
Electrical Requirements
200-V centrifuge
180–220 VAC, 24 A, 50/60 Hz
230-V centrifuge
207–253 VAC, 24 A, 50/60 Hz
208/240-V centrifuge
187–229/216–264 VAC, 24 A, 50 Hz
To reduce the risk of electrical shock, this centrifuge uses a permanently attached 1.8-m (6-ft)
three-wire UL/CSA approved electrical cord and plug to connect the centrifuge to earth-ground.
(Contact your local Beckman Coulter office for specific information regarding local plug
requirements.) To preserve this safety feature:
• Power to the centrifuge should originate directly from a main power line transformer at a
power source known to be clear of erratic loads, spikes, and electromagnetic interference.
Make sure there are properly rated thermal circuit breakers at the wall service panel to protect
the centrifuge circuit. If fuses must be used instead of the specified circuit breakers, the fuses
may require a rating of greater than 30 amperes. Figure A.2 shows the power connection.
Figure A.2 Single-Phase Power Connection
1
2
4
3
1
1. 30-ampere Circuit Breaker
2. Wall Outlet: Hubell 9930,
Bryant 96-30-FR, or Equivalent
(NEMA 6-30 R)
3. Earth-Ground
4. Measured Line Voltage
WARNING
To reduce the risk of electrical shock, this equipment uses a three-wire or five-wire
electrical cord and plug to connect the centrifuge to earth-ground. to preserve
this safety feature, make sure that the matching wall outlet receptacle is properly
wired and earth-grounded. Check that the line voltage agrees with the voltage
listed on the name rating plate affixed to the centrifuge.
• The Mains (power) plug is the disconnect device and must remain easily accessible.
• Position the centrifuge so that it is easy to remove the Mains (power) plug from the outlet
receptacle.
• Never use a three-to-two wire plug adapter.
PN SJ-IM-8AC
A-3
A
Preinstallation Requirements
Securing the Centrifuge to the Floor
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
• If there is any question about voltage, have a qualified service person measure it under load
while the drive is operating.
To ensure safety the centrifuge should be wired to a remote emergency switch in order to
disconnect the centrifuge from the main power source in case of a malfunction.
Securing the Centrifuge to the Floor
Avanti J series centrifuges are certified to meet the requirements of the European CE mark. To meet
these requirements, the centrifuge must be secured to the floor using the anchoring hardware
shipped with the instrument. This will prevent the centrifuge from moving in the unlikely event of
a rotor mishap.
Complete instructions for installing the anchoring kit are packaged with the hardware, which is
shipped with the centrifuge. The instructions (publication SJ-TB-002) include a full-size template to
be used as a guide for drilling holes in the floor. Refer to this document for additional installation
instructions.
NOTE Beckman Coulter representatives are not equipped to drill holes in your floor. The holes must be drilled
before your scheduled installation.
Bio-Safety Level 3 Installation
For laboratories with epoxy aggregate floors, such as BSL-3 labs, a non-invasive installation kit
(393316) is available. The kit which consists of an adhesive-backed mounting plate, is CSA certified
for use on epoxy aggregate floors only.
A-4
PN SJ-IM-8AC
Beckman Coulter, Inc.
Avanti J-E Centrifuge Warranty
Subject to the exceptions and upon the conditions specified below Beckman Coulter, Inc. agrees to
correct either by repair, or, at its election, by replacement, any defects of material or workmanship
which develop within one (1) year (2 years for the drive motor) after delivery of the Avanti J-E
Centrifuge (the product), to the original buyer by Beckman Coulter or by an authorized
representative, provided that investigation and factory inspection by Beckman Coulter discloses
that such defect developed under normal and proper use.
Some components and accessories by their nature are not intended to and will not function for as
long as one (1) year. If any such component or accessory fails to give reasonable service for a
reasonable period of time, Beckman Coulter will repair or, at its election, replace such component
or accessory. What constitutes either reasonable service and a reasonable period of time shall be
determined solely by Beckman Coulter.
Replacement
Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the
factory, transportation charges prepaid, and will be returned to Buyer with the transportation
charges collect unless the product is found to be defective, in which case Beckman Coulter will pay
all transportation charges.
Beckman Coulter makes no warranty concerning products or accessories not manufactured by it.
In the event of failure of any such product or accessory, Beckman Coulter will give reasonable
assistance to the Buyer in obtaining from the respective manufacturer whatever adjustment is
reasonable in light of the manufacturer’s own warranty.
Conditions
Beckman Coulter shall be released from all obligations under all warranties, either expressed or
implied, if the product covered hereby is repaired or modified by persons other than its own
authorized service personnel, unless such repair by others is made with the written consent of
Beckman Coulter, or unless such repair in the sole opinion of Beckman Coulter is minor, or unless
such modifications is merely the installation of a new Beckman Coulter plug-in component for such
product.
Disclaimer
IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF
FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER, INC.
SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND
WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR,
MAINTENANCE, OR REPLACEMENT OF THE PRODUCT.
PN SJ-IM-8AC
Warranty-1
Beckman Coulter, Inc. Avanti J-E Centrifuge Warranty
Warranty-2
PN SJ-IM-8AC
English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어
Symbol
Symbol
Simbolo
Symbole
Simbolo
Simbole
символ
符号
記号
상징
Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목
Dangerous voltage
Gefährliche electrische Spannung
Voltaje peligroso
Courant haute tension
Pericolo: alta tensione
Tensão perigosa
Опасное напряжение тока
危险电压
危険な電圧
위험한 전압
Caution, consult accompanying documents
Vorsicht, konsultieren Begleitdokumente
Atención, consulta documentos adjuntos
Attention, consultent des documents
d'accompagnement
Attenzione, consulta i documenti di
accompagnamento
Cuidado, ulta documentos adjuntos
Внимание, советует с сопроводительными
документами
注意, 咨询附属单证
注意, 伴う文書に相談しなさい
주의, 동반 문서를 상담하십시오
Biohazard
Potentiell infektiösem Material
Riesgo biológico
Risque biologiqu
Pericolo biologico
Material infeccioso potencial
биологической опасности
可能的传染性物
潜在的な感染性物質
전염하는 물자
On (power)
Ein (Netzverbindung)
Encendido
Marche (mise sous tension)
Acceso (sotto tensione)
Fora (o poder)
На (мощности)
开 (电源)
ン (電源)
에 (힘)
Off (power)
Aus (Netzverbindung)
Apagado
Arrêt (mise sous tension)
Spento (fuori tensione)
Fora de (poder)
С (сила)
(电源)
ン (電源)
떨어져 (힘)
Protective earth (ground)
Schutzleiteranschluß
Puesta a tierra de protección
Liaison à la terre
Collegamento di protezione a terra
Terra de proteção (terra)
Защитное заземление (земля)
保护接地
保護アース (接地)
방어적인 지구 (지상)
Earth (ground)
Erde (Masse)
La tierra (suelo)
Terre (sol)
Scarica a terra
Terra
Земли
接地
アース (接地)
지구 (지상)
Manufacturer
Hersteller
Fabricante
Fabricant
Fabbricante
Fabricante
производитель
制造商
メーカー
제조자
Consult Instructions for Use
Konsultieren Sie Anwendungsvorschriften
Consulte las instrucciones para el uso
Consultez les instructions pour l'usage
Consulti le istruzioni per uso
Consulte instruções para o uso
Советуйте с инструкциями для пользы
咨询使用说明书
使用説明に相談しなさい
사용 설명을 상담하십시오
Related Documents
Rotors and Tubes for
Beckman Coulter J2, J6,and
Avanti J Series Centrifuges
PN JR-IM-10
• Rotors
• Tubes, Bottles, and
Accessories
• Using Tubes and
Accessories
• Using Fixed-Angle Rotors
• Using Swinging-Bucket
Rotors
• Using Vertical-Tube and
Rack Type Rotors
• Care and Maintenance
• Chemical Resistances
• Temperature
Compensation Tables
• Gradient Materials
• Blood Component
Separation
High-Performance and HighCapacity Centrifuges Catalog
PN BR-8102
Available at
www.beckmancoulter.com
Available in electronic pdf or
CD-ROM by request.
Chemical Resistances for
Beckman Coulter
Centrifugation Products
PN IN-175
Available in hard copy or
electronic pdf by request.
Available at
www.beckmancoulter.com
Anchoring Kit Installation
Instructions for the Avanti J-E
Centrifuge
PN SJ-TB-002
Available in hard copy only.
www.beckmancoulter.com
© 2013 Beckman Coulter, Inc.
All Rights Reserved