Agilent 1100 Series Variable Wavelength Detector

Agilent 1100 Series
Variable Wavelength
Detector
Reference Manual
s1
Notices
© Agilent Technologies, Inc. 2002
Warranty
No part of this manual may be reproduced in
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into a foreign language) without prior agreement and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
The material contained in this document is provided “as is,” and is subject to being changed, without notice,
in future editions. Further, to the maximum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a particular purpose. Agilent shall not be
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consequential damages in connection with the furnishing, use, or performance of this document or of any
information contained herein. Should
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terms covering the material in this
document that conflict with these
terms, the warranty terms in the separate agreement shall control.
Manual Part Number
G1314-90003
Edition
Edition 07/02
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn, Germany
Software Revision
Technology Licenses
This guide is valid for A.01.xx revisions of
the Agilent 1100 Series Variable Wavelength Detector software, where xx refers to
minor revisions of the software that do not
affect the technical accuracy of this guide.
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accordance with the terms of such license.
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2
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must negotiate with Agilent to establish
acceptable terms in a written agreement
executed by all relevant parties.
Safety Notices
CAU TI O N
A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
to the product or loss of important
data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
WA RN ING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly performed or adhered to, could result
in personal injury or death. Do not
proceed beyond a WARNING
notice until the indicated conditions are fully understood and
met.
1100 Series Variable Wavelength Detector Reference Manual
In This Guide…
1
Installing the Variable Wavelength Detector
How to install the variable wavelength detector
2
How to optimize the detector
How to select the detector parameters and flow cell
3
Troubleshooting and Test Functions
This chapter describes the detector’s built in troubleshooting
and test functions.
4
Repairing the Variable Wavelength Detector
Instructions on how to repair the variable wavelength detector
5
Identifying Parts and Materials
Detailed illustrations and listings for parts and materials
identification for the variable wavelength detector
6
Introduction to the Variable Wavelength Detector
An introduction to the detector, istrument overview, theory of
operation, external communication and internal connectors
7
Control Module Screens for the Agilent 1100 Variable Wavelength
Detector
This chapter is intended to introduce an operator to the screens
available for operation of the Agilent 1100 variable wavelength
detector (VWD) with the Agilent 1100 control module.
Please use the manual of control module for further detailed
reference.
8
Specifications
Performance specifications of the variable wavelength detector
1100 Series Variable Wavelength Detector Reference Manual
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4
1100 Series Variable Wavelength Detector Reference Manual
Contents
1
Installing the Variable Wavelength Detector
Site Requirements
12
Physical Specifications
14
Unpacking the Detector
15
Optimizing the Stack Configuration
Installing the Detector
19
Flow Connections to the Detector
2
17
22
How to optimize the detector
Optimizing the Detector Performance 28
Match the Flow Cell to the Column 28
Set the Detector Parameters 31
3
Troubleshooting and Test Functions
Overview of the Detector’s Indicators and Test Functions
34
Status Indicators 35
Power Supply Indicator 35
Detector Status Indicator 36
Error Messages 37
Timeout 38
Shutdown 39
Remote Timeout 40
Sychronization Lost 41
Leak 42
Leak Sensor Open 43
1100 Series Variable Wavelength Detector Reference Manual
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Leak Sensor Short 44
Compensation Sensor Open 45
Compensation Sensor Short 46
Fan Failed 47
Open Cover 48
Lamp Current Missing 49
Lamp Voltage Missing 50
Lamp Ignition Failed 51
Heater Current Missing 52
Calibration Failed 53
Holmium Oxide Test Failed 54
Test Failed 55
Wavelength Check Failed 56
Filter Check Failed 57
Wavelength Calibration
58
When to Calibrate the Detector
59
Zero-Order Calibration 60
Calibration Procedure 60
656-nm Wavelength Calibration
Calibration Procedure 61
61
Test Functions 62
Lamp-on Routine 63
Checking the Photocurrent 64
Holmium Oxide Test 65
Intensity Test 67
Dark Current Test 69
DAC Test 71
Grating Motor Test 72
Filter Motor Test 73
Test Chromatogram 74
Diagnostic Signal Output 76
6
1100 Series Variable Wavelength Detector Reference Manual
Service Dialog 78
Signal Descriptions
4
80
Repairing the Variable Wavelength Detector
Introduction into Repairing the Variable Wavelength Detector
84
Overview of the Repairing of the Variable Wavelength
Detector 87
Simple Repairs 88
Exchanging a Lamp
89
Exchanging a Flow Cell
91
Repairing the Flow Cells
94
Using the Cuvette Holder
97
Correcting Leaks 100
Replacing Leak Handling System Parts
101
Exchanging Internal Parts 102
Removing the Top Cover and Top Foams
103
Exchanging the Processor Board 106
Entering the Serial Number using the Control Module
Entering the Serial Number using the
Agilent ChemStation 110
Exchanging the Fan
111
Repairs in the Optical Unit 113
Exchanging the Source Lens Assembly
114
Exchanging the Filter Assembly
117
Removing the Optical Unit 120
Exchanging the Leak Sensor 122
Exchanging the Power Supply 124
Replacing Status Light Pipe 128
Installing the Optical Unit 129
Installing the Foam and the Top Cover
131
1100 Series Variable Wavelength Detector Reference Manual
109
7
Assembling the Main Cover 133
Replacing the Interface Board 134
Replacing the Detector’s Firmware 135
5
Identifying Parts and Materials
Overview of Main Assemblies
138
Optical Unit and Fan Assembly
140
Source Lens and Filter Assembly
Standard Flow Cell (G1314-60086)
142
Standard Flow Cell (G1314-60080)
144
Micro Flow Cell
146
Semimicro Flow Cell
148
High Pressure Flow Cell
Cuvette Holder
152
Control Module
153
Sheet Metal Kit
154
Plastic Parts
Foam Parts
150
155
156
Power and Status Light Pipes
Leak Parts
Accessory Kit
8
141
157
158
159
1100 Series Variable Wavelength Detector Reference Manual
Cable Overview 160
Analog Cables 162
Remote Cables 165
BCD Cables 171
Auxiliary Cable 174
CAN Cable 175
External Contact Cable
RS-232 Cable Kit
LAN Cables
6
176
177
178
Introduction to the Variable Wavelength Dector
Introduction to the Detector
Optical System Overview
Electrical Connections
Instrument Layout
180
181
186
188
Early Maintenance Feedback (EMF)
EMF Counter 189
Using the EMF Counters 189
The Electronics
189
191
Detector Main Board (VWM)
192
Firmware Description 197
Firmware Updates 198
Raw Data Conversion to Absorbance
Optional Interface Boards
BCD Board 201
LAN Board 202
1100 Series Variable Wavelength Detector Reference Manual
199
200
9
Agilent 1100 Series Interfaces 203
Analog Signal Output 204
GPIB Interface 204
CAN Interface 204
Remote Interface 205
RS-232C 206
Setting the 8-bit Configuration Switch 208
GPIB Default Addresses 209
Communication Settings for RS-232C Communication
Forced Cold Start Settings 211
Stay-Resident Settings 212
The Main Power Supply Assembly
7
213
Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Major keys on the Agilent 1100 Control Module
Screens available from the Analysis screen
227
Screens available from the Records screen
229
235
Specifications
Performance Specifications
10
216
217
Screens available from the System screen
Diagnostics and Tests
8
210
242
1100 Series Variable Wavelength Detector Reference Manual
A
Safety Information
General Safety Information
246
Lithium Batteries Information
Radio Interference
Sound Emission
UV-Radiation
249
250
251
252
Solvent Information
253
Declaration of Conformity for HOX2 Filter
Agilent Technologies on Internet
254
255
Index
1100 Series Variable Wavelength Detector Reference Manual
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12
1100 Series Variable Wavelength Detector Reference Manual
Agilent 1100 Series Variable Wavelength Detector
Reference Manual
1
Installing the Variable Wavelength
Detector
Site Requirements 12
Physical Specifications 14
Unpacking the Detector 15
Optimizing the Stack Configuration 17
Installing the Detector 19
Flow Connections to the Detector 22
Agilent Technologies
11
1
Installing the Variable Wavelength Detector
Site Requirements
A suitable environment is important to ensure optimal performance of the
detector.
Power Consideration
The detector power supply has wide ranging capabilities (see Table 1 on
page 14). It accepts any line voltage in the above mentioned range.
Consequently, there is no voltage selector in the rear of the detector. There
are also no externally accessible fuses, because automatic electronic fuses are
implemented in the power supply.
WA RN ING
To disconnect the detector from line, unplug the power cord. The power supply still
uses some power, even if the power switch on the front panel is turned off.
WA RN ING
Shock hazard or damage of your instrumentation can result, if the devices are
connected to a line voltage higher than specified.
Power Cords
Different power cords are offered as options with the detector. The female end
of the power cords is identical. It plugs into the power-input socket at the rear
of the detector. The male end of each power cord is different and designed to
match the wall socket of a particular country or region.
ac
12
WA RN ING
Never operate your instrumentation from a power outlet that has no ground
connection. Never use a power cord other than the Agilent Technologies power cord
designed for your region.
WA RN ING
Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
1100 Series Variable Wavelength Detector Reference Manual
Installing the Variable Wavelength Detector
1
Bench Space
The detector dimensions and weight (see Table 1 on page 14) allow to place
the instrument on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inch) of space on either side and approximately 8 cm (3.1 inches)
in the rear for air circulation and electric connections.
If the bench should carry a Agilent 1100 Series system, make sure that the
bench is designed to bear the weight of all modules.
The detector should be operated in a horizontal position.
Environment
Your detector will work within specifications at ambient temperatures and
relative humidity as described in Table 1 on page 14.
ASTM drift tests require a temperature change below 2 ∞C/hour (3.6 ∞F/hour)
measured over one hour period. Our published drift specification (refer also to
“Performance Specifications" on page 242) is based on these conditions.
Larger ambient temperature changes will result in larger drift.
Better drift performance depends on better control of the temperature
fluctuations. To realize the highest performance, minimize the frequency and
the amplitude of the temperature changes to below 1 ∞C/hour (1.8 ∞F/hour).
Turbulences around one minute or less can be ignored.
CAU TI O N
Do not store, ship or use your detector under conditions where temperature
fluctuations could cause condensation within the detector. Condensation will damage
the system electronics. If your detector was shipped in cold weather, leave it in its box
and allow it to warm up slowly to room temperature to avoid condensation.
1100 Series Variable Wavelength Detector Reference Manual
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1
Installing the Variable Wavelength Detector
Physical Specifications
Table 1
Physical Specifications
Type
Specification
Weight
11 kg
25 lbs
Dimensions
(height × width × depth)
140 × 345 × 435 mm
5.5 × 13.5 × 17 inches
Line voltage
100–120 or 220–240 VAC,
Line frequency
50 or 60 Hz, ± 5%
Power consumption
220 VA / 85 W / 290 BTU
Ambient operating temperature
0–55 ∞C (32–131 ∞F)
Ambient non-operating temperature
-40–70 ∞C (-4–158 ∞F)
Humidity
< 95%, at 25–40 ∞C (77–104 ∞F)
Operating altitude
Up to 2000 m (6500 ft)
Non-operating altitude
Up to 4600 m (14950 ft)
Safety standards: IEC, CSA, UL, EN
Installation Category II, Pollution Degree 2
14
Comments
± 10%
Wide-ranging capability
Maximum
Non-condensing
For storing the instrument
1100 Series Variable Wavelength Detector Reference Manual
1
Installing the Variable Wavelength Detector
Unpacking the Detector
Damaged Packaging
If the delivery packaging shows signs of external damage, please call your
Agilent Technologies sales and service office immediately. Inform your service
representative that the detector may have been damaged during shipment.
CAU TI O N
If there are signs of damage, please do not attempt to install the detector.
Delivery Checklist
Ensure all parts and materials have been delivered with the detector. The
delivery checklist is shown below. Please report missing or damaged parts to
your local Agilent Technologies sales and service office.
Table 2
Variable Wavelength Detector Checklist
Description
Quantity
Variable wavelength detector
1
Power cable
1
CAN cable (Part number 5181-1516)
1
Flow cell
As ordered
Reference Manual
1
Accessory kit (see Table 3 on page 16)
1
b
1100 Series Variable Wavelength Detector Reference Manual
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1
Installing the Variable Wavelength Detector
Table 3
16
Accessory Kit Contents
Description
Part Number
Quantity
Accessory kit
G1314-68705
PEEK outlet capillary kit
5062-8535
1
Fitting male PEEK
0100-1516
1
Hex key 1.5 mm
8710-2393
1
Hex key 4 mm
8710-2392
1
Wrench open end 1/4 – 5/16 inch
8710-0510
1
Wrench open end 4 mm
8710-1534
1
ESD wrist strap
9300-1408
1
1100 Series Variable Wavelength Detector Reference Manual
1
Installing the Variable Wavelength Detector
Optimizing the Stack Configuration
If your detector is part of a complete Agilent 1100 Series system, you can
ensure optimum performance by installing the following configuration. This
configuration optimizes the system flow path, ensuring minimum delay
volume.
Solvent cabinet
Vacuum degasser
Pump
Control module
Autosampler
Column compartment
Detector
Figure 1
Recommended Stack Configuration (Front View)
1100 Series Variable Wavelength Detector Reference Manual
17
1
Installing the Variable Wavelength Detector
Remote cable
CAN Bus cable
CAN Bus cable
AC power
Analog
signal to
recorder
GPIB or LAN
to LC
ChemStation
Figure 2
18
Recommended Stack Configuration (Rear View)
1100 Series Variable Wavelength Detector Reference Manual
1
Installing the Variable Wavelength Detector
Installing the Detector
Preparations
Parts required
Locate bench space.
Provide power connections.
Unpack the detector.
Detector
Power cord, for other cables see text below and “Cable Overview" on page 160
ChemStation and/or Control Module G1323A/B
1 Install the LAN interface board in the detector (if required), see “Replacing
the Interface Board" on page 134.
2 Place the detector in the stack or on the bench in a horizontal position.
3 Ensure the line power switch at the front of the detector is OFF.
Status indicator
green/yellow/red
Line power switch
with green light
Figure 3
Front View of Detector
NO TE
The figure above shows the flow cell already installed. The flow cell area is closed with a
metal cover. The flow cell has to be installed as described in “Flow Connections to the
Detector" on page 22.
1100 Series Variable Wavelength Detector Reference Manual
19
1
Installing the Variable Wavelength Detector
4 Connect the power cable to the power connector at the rear of the detector.
5 Connect the CAN cable to other Agilent 1100 Series modules.
6 If a Agilent ChemStation is the controller, connect either
• the GPIB cable to the detector or
• the LAN connection to the LAN interface board in the detector.
7 Connect the analog cable (optional).
NO TE
If a Agilent 1100 DAD/MWD is in the system, the LAN/GPIB should be connected to the
DAD/MWD (due to higher data load).
8 Connect the APG remote cable (optional) for non-Agilent 1100 Series
instruments.
9 Turn on power by pushing the button at the lower left-hand side of the
detector. The status LED should be green.
Security lever
Interface board
Analog signals
APG remote
RS-232C
CAN
GPIB
Power
Configuration switch
Figure 4
20
Rear View of Detector
1100 Series Variable Wavelength Detector Reference Manual
1
Installing the Variable Wavelength Detector
NO TE
The detector is turned on when the line power switch is pressed and the green indicator
lamp is illuminated. The detector is turned off when the line power switch is protruding and
the green light is off.
WA RN ING
To disconnect the detector from line, unplug the power cord. The power supply still
uses some power, even if the power switch at the front panel is turned off.
NO TE
The detector was shipped with default configuration settings. To change these settings,
“Setting the 8-bit Configuration Switch" on page 208
1100 Series Variable Wavelength Detector Reference Manual
21
1
Installing the Variable Wavelength Detector
Flow Connections to the Detector
Preparations
Parts required
WA RN ING
NO TE
Detector is installed in the LC system.
Other modules
Parts from accessory kit, see “Accessory Kit Contents" on page 16
Two wrenches 1/4–5/16 inch for capillary connections
When working with solvents please observe appropriate safety procedures (for
example, goggles, safety gloves and protective clothing) as described in the
material handling and safety data sheet supplied by the solvent vendor, especially
when using toxic or hazardous solvents.
The flow cell is shipped with a filling of isopropanol (also recommended when the
instrument and/or flow cell is shipped to another location). This is to avoid breakage due to
subambient conditions.
1 Press the release buttons and remove the front cover to
have access to the flow cell area.
22
2 Remove the metal cover and install the flow cell. Tighten
the cell screws.
1100 Series Variable Wavelength Detector Reference Manual
Installing the Variable Wavelength Detector
1
3 Assemble the column-detector capillary.
Depending on the flow cell type it may be a PEEK or SST
capillary.
4 Connect the newly assembled fitting of the capillary to the
inlet connector.
5 Connect the other end of the capillary to the column.
6 Connect the PEEK waste capillary to the outlet connector.
1100 Series Variable Wavelength Detector Reference Manual
23
1
Installing the Variable Wavelength Detector
7 Establish a flow and observe for leakage.
8 Replace the front cover.
The installation of the detector is now complete.
24
1100 Series Variable Wavelength Detector Reference Manual
Installing the Variable Wavelength Detector
1100 Series Variable Wavelength Detector Reference Manual
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25
1
26
Installing the Variable Wavelength Detector
1100 Series Variable Wavelength Detector Reference Manual
Agilent 1100 Series Variable Wavelength Detector
Reference Manual
2
How to optimize the detector
Optimizing the Detector Performance 28
Agilent Technologies
27
2
How to optimize the detector
Optimizing the Detector Performance
The detector has a variety of parameters that can be used to optimize
performance.
The information below will guide you on how to get the best detector
performance. Follow these rules as a start for new applications. It gives a
rule-of-thumb for optimizing the detector parameters.
Match the Flow Cell to the Column
Figure 5 recommends the flow cell that matches the column used. If more than
one selection is appropriate, use the larger flow cell to get the best detection
limit. Use the smaller flow cell for best peak resolution.
Column length
Typical peak
width
<= 5 cm
0.025 min
10 cm
0.05 min
20 cm
0.1 min
>= 40 cm
0.2 min
Recommended flow cell
Micro
flow cell
Semimicro
flow cell
Standard
flow cell
Typical flow rate
Internal column diameter
Figure 5
28
0.05 – 0.2 ml/min 0.2 – 0.4 ml/min
0.4 – 0.8 ml/min
1 – 2 ml/min
1.0 mm
3.0 mm
4.6 mm
2.1 mm
Choosing a Flow Cell
1100 Series Variable Wavelength Detector Reference Manual
2
How to optimize the detector
Flow Cell Path Length
Lambert-Beer’s law shows a linear relationship between the flow cell path
length and absorbance.
I
bsorbance = ñ log T = log ---0- = ε ⋅ C ⋅ d
I
where
Tis the transmission, defined as the quotient of the intensity of the
transmitted light I divided by the intensity of the incident light, I0,
εis the extinction coefficient, which is a characteristic of a given substance
under a precisely-defined set of conditions of wavelength, solvent,
temperature and other parameters,
Cis the concentration of the absorbing species (usually in g/l or mg/l), and
dis the path length of the cell used for the measurement.
Therefore, flow cells with longer path lengths yield higher signals. Although
noise usually increases little with increasing path length, there is a gain in
signal-to-noise ratio. For example, in Figure 6 on page 30 the noise increased
by less than 10 % but a 70 % increase in signal intensity was achieved by
increasing the path length from 6 mm to 10 mm.
When increasing the path length, the cell volume usually increases — in our
example from 5 – 13 µl. Typically, this causes more peak dispersion. As
Figure 6 on page 30 demonstrates, this did not affect the resolution in the
gradient separation in our example.
As a rule-of-thumb the flow cell volume should be about 1/3 of the peak
volume at half height. To determine the volume of your peaks, take the peak
width as reported in the integration results multiply it by the flow rate and
divide it by 3).
1100 Series Variable Wavelength Detector Reference Manual
29
2
How to optimize the detector
Analysis of pesticide standard
Absorbance
6-mm optical path length
10-mm optical path length
Time (min)
Figure 6
Influence of Cell Path Length on Signal Height
Traditionally LC analysis with UV detectors is based on comparing
measurements with internal or external standards. To check photometric
accuracy of the Agilent 1100 VWD it is necessary to have more precise
information on path lengths of the VWD flow cells.
The correct response is:
expected response * correction factor
Please find below the details of the Agilent 1100 VWD flow cells:
Table 4
Correction factors for Agilent 1100 VWD flow cells
Flow cell type
Cell
volume
Part number
Path length
(nominal)
Path length
(actual)
Correction
factor
Standard flow cell
14 µl
G1314-60080
10 mm
10.15 ± 0.19 mm
10/10.15
Semimicro flow cell
5 µl
G1314-60083
6 mm
6.10 ± 0.19 mm
6/6.19
Micro flow cell kit
1 µl
G1314-60081
5 mm
4.80 ± 0.19 mm
5/4.8
High Pressure flow cell
14 µl
G1314-60082
10 mm
10.00 ± 0.19 mm
6/5.75
NO TE
30
However you have to be aware that there are additional tolerance of gasket thickness and
its compression ratio which is supposed to be very small in comparison with the machining
tolerance.
1100 Series Variable Wavelength Detector Reference Manual
2
How to optimize the detector
Set the Detector Parameters
1 Set peakwidth as close as possible to the width (at half height) of a narrow
peak of interest.
2 Choose the sample wavelength.
• at a longer wavelength than the cut-off wavelength of the mobile phase,
• at a wavelength where the analytes have strong absorptivity if you want
to get the lowest possible detection limit,
• at a wavelength with moderate absorptivity if you work with high
concentrations, and
• preferably where the spectrum is flat for better linearity.
3 Consider to use time-programming to further optimization.
1100 Series Variable Wavelength Detector Reference Manual
31
2
32
How to optimize the detector
1100 Series Variable Wavelength Detector Reference Manual
Agilent 1100 Series Variable Wavelength Detector
Reference Manual
3
Troubleshooting and Test Functions
Overview of the Detector’s Indicators and Test Functions 34
Status Indicators 35
Error Messages 37
Wavelength Calibration 58
When to Calibrate the Detector 59
Zero-Order Calibration 60
656-nm Wavelength Calibration 61
Test Functions 62
Service Dialog 78
Agilent Technologies
33
3
Troubleshooting and Test Functions
Overview of the Detector’s Indicators and Test Functions
Status Indicators
The detector is provided with two status indicators which indicate the
operational state (prerun, run, and error states) of the detector. The status
indicators provide a quick visual check of the operation of the detector (see
page 35).
Error Messages
In the event of an electronic, mechanical or hydraulic failure, the detector
generates an error message in the user interface. For each message, a short
description of the failure, a list of probable causes of the problem, and a list of
suggested actions to fix the problem are provided (see “Error Messages" on
page 37).
Wavelength Recalibration
Wavelength recalibration is recommended after repair of internal components,
and on a regular basis to ensure correct operation of the detector. The
detector uses the zero-order maximum and the deuterium alpha-emission line
for wavelength calibration (see “Wavelength Calibration" on page 58).
Test Functions
A series of test functions are available for troubleshooting and operational
verification after exchanging internal components (see “Test Functions" on
page 62).
34
1100 Series Variable Wavelength Detector Reference Manual
Troubleshooting and Test Functions
3
Status Indicators
Two status indicators are located on the front of the detector. The lower left
indicates the power supply status, the upper right indicates the detector
status.
Status indicator
green/yellow/red
Line power switch
with green light
Figure 7
Location of Status Indicators
Power Supply Indicator
The power supply indicator is integrated into the main power switch. When
the indicator is illuminated (green) the power is ON.
1100 Series Variable Wavelength Detector Reference Manual
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3
Troubleshooting and Test Functions
Detector Status Indicator
The detector status indicator indicates one of four possible instrument
conditions:
• When the status indicator is OFF (and power switch light is on), the
instrument is in a prerun condition, and is ready to begin an analysis.
• A green status indicator, indicates the instrument is performing an analysis
(run mode).
• A yellow indicator indicates a not-ready condition. The instrument is in a
not-ready state when it is waiting for a specific condition to be reached or
completed (for example, immediately after changing a setpoint), or while a
self-test procedure is running.
• An error condition is indicated when the status indicator is red. An error
condition indicates the instrument has detected an internal problem which
affects correct operation of the instrument. Usually, an error condition
requires attention (for example, leak, defective internal components). An
error condition always interrupts the analysis.
36
1100 Series Variable Wavelength Detector Reference Manual
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Troubleshooting and Test Functions
Error Messages
Error messages are displayed in the user interface when an electronic,
mechanical, or hydraulic (flow path) failure occurs which requires attention
before the analysis can be continued (for example, repair, or exchange of
consumables is necessary). In the event of such a failure, the red status
indicator at the front of the module is switched on, and an entry is written
into the instrument logbook.
This section describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
1100 Series Variable Wavelength Detector Reference Manual
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3
Troubleshooting and Test Functions
Timeout
The timeout threshold was exceeded.
Probable Causes
• The analysis was completed successfully, and the time0ut function switched
off the pump as requested.
• A not-ready condition was present during a sequence or multiple-injection
run for a period longer than the timeout threshold.
Suggested Actions
✔ Check the logbook for the occurrence and source of a not-ready condition.
Restart the analysis where required.
38
1100 Series Variable Wavelength Detector Reference Manual
3
Troubleshooting and Test Functions
Shutdown
An external instrument has generated a shut-down signal on the remote line.
The detector continually monitors the remote input connectors for status
signals. A LOW signal input on pin 4 of the remote connector generates the
error message.
Probable Causes
• Leak detected in an external instrument with a remote connection to the
system.
• Shut-down in an external instrument with a remote connection to the
system.
• The degasser failed to generate sufficient vacuum for solvent degassing.
Suggested Actions
✔ Fix the leak in the external instrument before restarting the pump.
✔ Check external instruments for a shut-down condition.
✔ Check the degasser module for an error condition. Refer to the Reference
Manual for the Agilent 1100 Series vacuum degasser.
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Remote Timeout
A not-ready condition is still present on the remote input.
When an analysis is started, the system expects all not-ready conditions (for
example, a not-ready condition during detector balance) to switch to run
conditions within one minute of starting the analysis. If a not-ready condition
is still present on the remote line after one minute the error message is
generated.
Probable Causes
• Not-ready condition in one of the instruments connected to the remote line.
• Defective remote cable.
• Defective components in the instrument showing the not-ready condition.
Suggested Actions
✔ Ensure the instrument showing the not-ready condition is installed
correctly, and is set up correctly for analysis.
✔ Exchange the remote cable.
✔ Check the instrument for defects (refer to the instrument’s reference
documentation).
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Sychronization Lost
During an analysis, the internal synchronization or communication between
one or more of the modules in the system has failed.
The system processors continually monitor the system configuration. If one or
more of the modules is no longer recognized as being connected to the system,
the error message is generated.
Probable Causes
• CAN cable disconnected.
• Defective CAN cable.
• Defective main board in another module.
Suggested Actions
✔ Ensure all the CAN cables are connected correctly.
✔ Switch off the system. Restart the system, and determine which module or
modules are not recognized by the system.
✔ Ensure all CAN cables are installed correctly.
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Leak
A leak was detected in the detector.
The signals from the two temperature sensors (leak sensor and
board-mounted temperature-compensation sensor) are used by the leak
algorithm to determine whether a leak is present. When a leak occurs, the leak
sensor is cooled by the solvent. This changes the resistance of the leak sensor
which is sensed by the leak-sensor circuit on the VWM board.
Probable Causes
• Loose fittings.
• Broken capillary.
• Leaking flow cell.
Suggested Actions
✔ Ensure all fittings are tight.
✔ Exchange defective capillaries.
✔ Exchange flow cell components.
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Leak Sensor Open
The leak sensor in the detector has failed (open circuit).
The current through the leak sensor is dependent on temperature. A leak is
detected when solvent cools the leak sensor, causing the leak-sensor current to
change within defined limits. If the current falls outside the lower limit, the
error message is generated.
Probable Causes
• Leak sensor not connected to the VWM board.
• Defective leak sensor.
Suggested Actions
✔ Ensure the leak sensor is connected correctly.
✔ Exchange the leak sensor.
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Leak Sensor Short
The leak sensor in the detector has failed (short circuit).
The current through the leak sensor is dependent on temperature. A leak is
detected when solvent cools the leak sensor, causing the leak-sensor current to
change within defined limits. If the current increases above the upper limit,
the error message is generated.
Probable Causes
• Defective leak sensor.
Suggested Actions
✔ Exchange the leak sensor.
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Compensation Sensor Open
The ambient-compensation sensor (NTC) on the VWM board in the detector
has failed (open circuit).
The resistance across the temperature compensation sensor (NTC) on the
VWM board is dependent on ambient temperature. The change in resistance is
used by the leak circuit to compensate for ambient temperature changes. If the
resistance across the sensor increases above the upper limit, the error
message is generated.
Probable Causes
• Defective VWM board.
Suggested Actions
✔ Exchange the VWM board.
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Compensation Sensor Short
The ambient-compensation sensor (NTC) on the VWM board in the detector
has failed (short circuit).
The resistance across the temperature compensation sensor (NTC) on the
VWM board is dependent on ambient temperature. The change in resistance is
used by the leak circuit to compensate for ambient temperature changes. If the
resistance across the sensor falls below the lower limit, the error message is
generated.
Probable Causes
• Defective VWM board.
Suggested Actions
✔ Exchange the VWM board.
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Fan Failed
The cooling fan in the detector has failed.
The hall sensor on the fan shaft is used by the VWM board to monitor the fan
speed. If the fan speed falls below two revolutions/second for more than five
seconds, the error message is generated.
Probable Causes
• Fan cable disconnected.
• Defective fan.
• Defective VWM board.
Suggested Actions
✔ Ensure the fan is connected correctly.
✔ Exchange fan.
✔ Exchange the VWM board.
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Open Cover
The foam (#2) has been removed.
The sensor on the VWM board detects when the top foam (#2) is in place. If the
foam is removed during operation, the lamp and grating drive power is
switched off, and the error message is generated.
Probable Causes
• Foam #2 removed.
Suggested Actions
✔ Replace the foam.
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Lamp Current Missing
The lamp anode current is missing.
The processor continually monitors the anode current drawn by the lamp
during operation. If the anode current falls below the lower current limit, the
error message is generated.
Probable Causes
• Lamp disconnected.
• Top foam removed while lamp is on.
• Defective lamp.
• Defective VWM board.
• Defective power supply.
Suggested Actions
✔ Ensure the lamp connector is seated firmly.
✔ Replace the top foam, and turn on the lamp.
✔ Exchange the lamp.
✔ Exchange the VWM board.
✔ Exchange the power supply.
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Lamp Voltage Missing
The lamp anode voltage is missing.
The processor continually monitors the anode voltage across the lamp during
operation. If the anode voltage falls below the lower limit, the error message is
generated.
Probable Causes
• Defective VWM board.
• Defective power supply.
Suggested Actions
✔ Exchange the VWM board.
✔ Exchange the power supply.
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Lamp Ignition Failed
The lamp failed to ignite.
The processor monitors the lamp current during the ignition cycle. If the lamp
current does not rise above the lower limit within 2 – 5 seconds, the error
message is generated.
Probable Causes
• Lamp not connected.
• Defective lamp.
• Defective VWM board.
• Defective power supply.
Suggested Actions
✔ Ensure the lamp is connected.
✔ Exchange the lamp.
✔ Exchange the VWM board.
✔ Exchange the power supply.
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Heater Current Missing
The lamp heater current in the detector is missing.
During lamp ignition, the processor monitors the heater current. If the current
does not rise above the lower limit within one second, the error message is
generated.
Probable Causes
• Lamp not connected.
• Ignition started without the top foam in place.
• Defective lamp.
• Defective VWM board.
• Defective power supply.
Suggested Actions
✔ Ensure the lamp is connected.
✔ Replace the top foam, and turn on the lamp.
✔ Exchange the lamp.
✔ Exchange the VWM board.
✔ Exchange the power supply.
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Calibration Failed
The intensity maximum was not found during wavelength calibration.
Calibration 0 Failed: Zero-order calibration failed.
Calibration 1 Failed: 656 nm calibration failed.
During zero-order and 656 nm calibration, the detector searches for the
intensity maximum. If the maximum is not detected within the scan range, the
error message is generated.
Probable Causes
• Lamp is off.
• Incorrect flow cell installation.
• Flow cell contamination or air bubbles.
• Intensity too low.
• Current step value too far from maximum.
• Misalignment of the grating assembly.
• Defective grating assembly.
• Defective VWM board.
Suggested Actions
✔ Switch on the lamp.
✔ Ensure the flow cell are installed correctly.
✔ Clean/replace flow cell windows or remove air bubbles.
✔ Replace lamp.
✔ Enter a different calibration step value (different scan range). Repeat the
calibration.
✔ Realign the grating.
✔ Run the grating-motor test to determine if the grating assembly is defective.
Exchange the grating assembly if required.
✔ Exchange the VWM board.
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Holmium Oxide Test Failed
The holmium oxide test in the detector has failed.
During the holmium test, the detector moves the holmium filter into the light
path, and compares the measured absorbance maxima of the holmium oxide
filter with expected maxima. If the measured maxima are outside the limits,
the error message is generated.
Probable Causes
• Defective or dirty flow cell.
• Defective filter motor assembly.
• Defective grating motor assembly.
Suggested Actions
✔ Ensure the flow cell is inserted correctly, and is free from contamination
(cell windows, buffers, and so on).
✔ Run the filter-motor test to determine if the grating motor assembly is
defective. Exchange the filter motor assembly.
✔ Run the grating-motor test to determine if the grating assembly is defective.
Exchange the grating motor assembly if required.
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Test Failed
The motor test has failed.
Test 0 Failed: Filter motor.
Test 1 Failed: Grating motor
During the motor tests, the detector moves the motor to the end position while
monitoring the end-position sensor. If the end position is not found, the error
message is generated.
Probable Causes
• Defective motor assembly.
Suggested Actions
✔ Run the motor test again to confirm the motor assembly is defective.
Exchange the grating motor assembly if required.
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Wavelength Check Failed
The automatic wavelength check after lamp ignition has failed.
When the lamp is switched on, the detector waits 1 minute to warm-up the
lamp. Then a check of the deuterium emission line (656 nm) via the reference
diode is performed. If the emission line is more than 3 nm away from 656 nm,
the error message is generated.
Probable Causes
• Calibration incorrect.
Suggested Actions
✔ Recalibrate the detector.
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Filter Check Failed
The automatic filter check after lamp ignition has failed.
When the lamp is switched on, the detector moves the cutoff filter into the
light path. If the filter is functioning correctly, a decrease in lamp intensity is
seen. If the expected intensity decrease is not detected, the error message is
generated.
Probable Causes
• Filter motor defective.
• Defective or missing filter.
Suggested Actions
✔ Run the filter motor test to determine if the motor is defective.
✔ Exchange the filter assembly.
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Wavelength Calibration
Wavelength calibration of the detector is done using the zero-order position
and 656 nm emission line position of the deuterium lamp. The calibration
procedure involves two steps. First the grating is calibrated on the zero-order
position. The stepper-motor step position where the zero-order maximum is
detected is stored in the detector. Next, the grating is calibrated against the
deuterium emission-line at 656 nm, and the motor position at which the
maximum occurs is stored in the detector.
In addition to the zero-order and 656 nm (alpha-emission line) calibration, the
beta-emission line at 486 nm and the three holmium lines are used for the
complete wavelength calibration process. These holmium lines are at
360.8 nm, 418.5 nm and 536.4 nm.
NO TE
The wavelength calibration takes about 3.5 minutes and is disabled within the first
10 minutes after ignition of the lamp because initial drift may distort the measurement.
When the lamp is turned on, the 656 nm emission line position of the
deuterium lamp is checked automatically, see “Lamp-on Routine" on page 63.
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When to Calibrate the Detector
The detector is calibrated at the factory, and under normal operating
conditions should not require recalibration. However, it is advisable to
recalibrate:
• after maintenance (flow cell or lamp),
• after repair of components in the optical unit,
• after exchange of the optical unit or VWM board,
• at a regular interval, at least once per year (for example, prior to an
Operational Qualification/Performance Verification procedure), and
• when chromatographic results indicate the detector may require
recalibration.
NO TE
Figure 8
The wavelength calibration takes about 3.5 minutes and is disabled within the first
10 minutes after ignition of the lamp.
Wavelength Calibration
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Zero-Order Calibration
The zero-order calibration function is used for the electrical calibration of the
zero-order beam of the monochromator.
The grating is driven by the stepper motor while the detector searches for the
intensity maximum occurring at the zero-order position. The zero-order
calibration values are stored in the instrument.
The deviation is displayed in nm on the user interface, see Figure 8 on
page 59.
Calibration Procedure
1 Install the flow cell.
2 Flush the flow cell with LC-grade water at 1 ml/min.
3 Run the zero-order calibration test.
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656-nm Wavelength Calibration
The wavelength calibration function is used to calibrate the monochrometer
against the 656 nm emission line of the deuterium lamp.
The detector searches for maximum light intensity in the region of the 656 nm
emission line. The 656 nm calibration values are stored in the instrument.
The deviation is displayed in nm on the user interface, see Figure 8 on
page 59.
Calibration Procedure
1 Install the flow cell.
2 Flush the flow cell with LC-grade water at 1 ml/min.
3 Run the 656-nm calibration test.
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Test Functions
The instrument provides the following test functions:
• holmium oxide test,
• lamp intensity test (lamp characteristics),
• ADC test,
• dark current test,
• DAC test,
• grating motor test, and
• filter motor test.
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Lamp-on Routine
When the lamp is turned on the following routine is implemented:
• During the ignition phase the grating position is reinitialized by moving the
grating position lever into the sensor position.
• After ignition, a 1-minute warm-up phase should stabilize the lamp.
• Check of wavelength setting at 656 nm emission line (must be within 3 nm).
This measurement is taken at the reference side only (to have no influence
from flow cell condition). If not correct then a message for recalibration is
displayed.
• Intensity check:
at 250 nm without cutoff filter (= value 1).
at 250 nm with cutoff filter (= value 2).
The result should be value 2 < value 1/16.
If not OK, the cutoff filter does not work correctly.
• Restore the set wavelength position.
• The detector should now be ready condition.
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Checking the Photocurrent
To identify problems with the flow cell (contaminated windows, air bubbles
and so on) you can use raw data from the sample and reference photodiode
(unfiltered and not logarithmic).
NO TE
The screen of the VWD-Tests on the control module in Figure 9 shows the wavelength and
photo current information. This feature is not available on control module firmware
revisions A.1.04 and below.
For all control module firmware revisions A.1.04 and below, use the “Service Dialog" on
page 78.
actual wavelength
Figure 9
actual intensity
counts (raw data)
Photocurrent Readings on Control Module
With a clean cell the counts for sample and reference are in the same range. If,
like in this example, the sample side shows much less, the flow cell might have
a problem due to
✔ dirty windows,
✔ absorbing solvents/compounds,
✔ not correctly mounted flow cell, or
✔ air bubbles
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Holmium Oxide Test
This test verifies the calibration of the detector against the three wavelength
maxima of the built-in holmium oxide filter. The test displays the difference
between the expected and measured maxima.
The test uses the following holmium maxima:
• 360.8 nm
• 418.5 nm
• 536.4 nm
NO TE
Figure 10
See also “Declaration of Conformity for HOX2 Filter" on page 254.
Holmium Test
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When to do the Test
• after recalibration,
• as part of the Operational Qualification/Performance Verification
procedure, or
• after flow cell maintenance or repair.
Interpreting the Results
The test is passed successfully when all three wavelengths are within ± 1 nm of
the expected value. This indicates the detector is calibrated correctly.
NO TE
The test results are currently available on the Agilent ChemStation only.
Holmium Oxide Test Failed
Probable Causes
• Detector not calibrated.
• Dirty or defective flow cell.
• Dirty or defective holmium oxide filter.
• Optical misalignment.
Suggested Actions
✔ Recalibrate the detector.
✔ Repeat the test with the flow cell removed. If the test is OK, exchange the
flow cell components.
✔ Run the holmium oxide filter test. If the test fails, exchange the filter
assembly.
✔ Realign the optical components.
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Intensity Test
The intensity test measures the intensity of the deuterium lamp over the full
VWD wavelength range (190 – 600 nm). The test can be used to determine the
performance of the lamp, and to check for dirty or contaminated flow cell
windows. When the test is started, the gain is set to zero. To eliminate effects
due to absorbing solvents, the test should be done with water in the flow cell.
The shape of the intensity spectrum is primarily dependent on the lamp,
grating, and diode characteristics. Therefore, intensity spectra will differ
slightly between instruments. Figure 11 shows a typical intensity test
spectrum.
Figure 11
Intensity Test
Intensity Test Evaluation (Agilent ChemStation only)
The Agilent ChemStation evaluates three values automatically and displays
the limits for each value, the average, the minimum and the maximum of all
data points and passed or failed for each value.
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Table 5
Intensity Limits
Range
Limit (counts)
High intensity
> 10000
Average intensity
> 5000
Lowest intensity
> 200
Test Failed
Probable Causes
• Absorbing solvent in flow cell.
• Dirty or contaminated flow cell.
• Dirty or contaminated optical components (source lens, mirrors, grating).
Suggested Actions
✔ Ensure the flow cell is filled with water.
✔ Repeat the test with the flow cell removed. If the test passes, exchange the
flow cell windows.
✔ Clean/replace optical components.
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Dark Current Test
This test determines the dark-current noise contribution of the front-end
electronics.
During the test, the lamp is switched off. The resulting signal corresponds to
the unfiltered dark-current noise contribution of the front-end electronics.
When the test is run, the signal is output to the detector analog output.
When to do the Test
• if the detector signal is noisy.
Interpreting the Results
The output signal of the ADC noise corresponds to AD counts with 1 V or 0.1 V
full scale setting. The ADC noise must be taken over a time of 10 minutes for
the results to be accepted.
Average counts should be less than 7900 counts
Dark Current Test Failed
Probable Causes
• Defective sample or reference diode.
• Defective sample or reference VWA board.
• Defective VWM board.
Suggested Actions
✔ Exchange the reference or sample diode.
✔ Exchange sample or reference VWA board.
✔ Exchange the VWM board.
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How to do it with the Agilent ChemStation
On the Agilent ChemStation the test is evaluated automatically and checked
against the limits.
VWD1 A, Wavelength=254 nm (DIAGNOSE\DGVWDDC.D)
mAU
-27.6
-27.7
-27.8
-27.9
-28
-28.1
0
0.2
0.4
0.6
VWD1 E, Unfiltered Sample (DIAGNOSE\DGVWDDC.D)
0.8
1
1.2
1.4
1.6
1.8
min
0
0.2
0.4
0.6
VWD1 F, Unfiltered Reference (DIAGNOSE\DGVWDDC.D)
0.8
1
1.2
1.4
1.6
1.8
min
0.8
1
1.2
1.4
1.6
1.8
min
counts
3475
3470
3465
3460
3455
counts
3510
3505
3500
3495
3490
0
Figure 12
0.2
0.4
0.6
Dark Current Noise
How to do it with the Control Module
On the Control Module the dark current counts are displayed as follows:
1 Enable the Dark Current Test.
2 Activate the Service Dialog (m-key, Service)
3 Select Signal 1 - Raw Sample.
4 Select Signal 2 - Raw Reference.
5 Observe the count readings for signal 1 and signal 2.
The displayed values should be less than 7900 counts.
The dark current signal can be displayed directly on the screen.
1 Select in VWD - Settings - Analog Output as Raw Sample or Raw Reference.
2 Press the Plot button and select the Analog signal in Setup.
3 Return to the Plot screen by pressing Done.
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DAC Test
This test determines correct operation of the digital-analog converter (DAC).
The test applies an AC voltage (10 µV) to the DC output of the DAC. The DAC
output is connected to the analog output connector at the rear of the detector.
Figure 13
DAC Test
When to do the Test
• if the detector signal is noisy or missing.
Interpreting the Results
The noise on the step should be less than 3 µV.
Probable Causes
• Defective VWM board.
Suggested Actions
✔ Exchange the VWM board.
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Grating Motor Test
This test moves the grating motor to the end position, and displays the
motor-position step difference. This checks the movement of the grating drive
mechanism.
When to do the Test
• if recalibration cannot be done successfully, or
• if the holmium test fails.
Interpreting the Results
Grating Motor Test Failed
Probable Causes
• Defective grating motor.
• Defective grating position sensor
• Defective VWM board.
Suggested Actions
✔ Exchange the grating motor
✔ Exchange the grating position sensor.
✔ Exchange the VWM board.
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Filter Motor Test
This control function is used to check the motion of second-order cut-off filter.
The cut-off filter returns to original position at the end of this control
function.
When to do the Test
• if the holmium test fails.
Grating Motor Test Failed
Probable Causes
• Defective filter motor
• Defective filter position sensor.
• Defective VWM board.
Suggested Actions
✔ Exchange the filter motor
✔ Exchange the filter position sensor.
✔ Exchange the VWM board.
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Test Chromatogram
A pre-defined test chromatogram on the VWM board is processed through
ADC like normal signals from the diodes and can be used to check the ADC
and the data handling system. The signal is available at the analog output and
on the GPIB.
NO TE
The run time of the chromatogram is depending on the setting for response time (peak
width). If no stop time is set the chromatogram will repeat continuously.
Table 6
Test Chromatogram Settings
Response time
Set Run Time
0.06 sec
0.8 min
0.12 sec
0.8 min
0.25 sec
0.8 min
0.50 sec
0.8 min
1.00 sec
1.6 min
2.00 sec
3.2 min
4.00 sec
6.4 min
8.00 sec
12.8 min
Control Module
1 Select the response time and stop time according to Table 6.
2 Select the function Enable test chromatogram in Tests - VWD and press
Execute.
3 Select the Plot button in the System screen and press the Setup button.
4 Select as Source the Signal (VWD), change the Y-range to - 10 to 300 mAU,
fit the Time Range to match Table 6 and press Done.
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5 If required, connect a recording device (for example, Agilent 3396A with
settings ATTN=9, CHSP=2, ZERO=10, AR REJ=106).
6 Press the Start button to start the run. On completion of the run, or when
the run is stopped, the test chromatogram is deactivated.
ADC1 A, Analog Output (TEST\TESTCR2S.D)
1.893
mV
350
300
1.501
250
200
2.868
0.983
150
100
50
0.5
Figure 14
1
1.5
2
2.5
3
min
Test Chromatogram on Integrator (response time 2 seconds)
Agilent ChemStation
1 Select the response time and stop time according to Table 6 on page 74.
2 Activate the test chromatogram by typing into the command line (or as
pre-run command): PRINT SENDMODULE$(LVWD,“SIMU:DFLT”)
3 Start the run. On completion of the run, or when the run is stopped, the test
chromatogram is deactivate.
VWD1 A, Wavelength=254 nm (TEST\TESTCR2S.D)
1.870
mAU
250
200
1.478
150
50
2.846
0.959
100
0
0
Figure 15
0.5
1
1.5
2
2.5
3
min
Test Chromatogram on Agilent ChemStation (response time 2 seconds)
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Diagnostic Signal Output
The detector provides an analog signal output for various detector signals for
diagnostic purposes.
Selecting and Plotting Signals
Some of the signals are available on the analog output only when running
specific tests (see following sections). Only one signal can be plotted at any
one time.
NO TE
All values in Table 8 on page 79 refer to the following settings on the user interface:
attenuation 1000 mAU,
zero offset 5%,
analog voltage range 1 V full scale.
The following signals are available on the analog signal output and the control
display (select Analog Out VWD as source signal in the Plot-Setup).
NO TE
For signal descriptions see “Signal Descriptions" on page 80.
Table 7
Diagnostic Signals on Analog Output
Signal
Analog Out
1 V is equal to
Constant zero offset
0 mV
Absorbance
1 AU
AU
Sample signal without reference
1 AU
AU
Reference signal without sample
1 AU
AU
Sample raw data
(unfiltered and not logarithmic)
2 counts
Counts
Reference raw data
(unfiltered and not logarithmic)
221 counts
Counts
Analog output test
zero offset + 10 µV step
Counts
Sample diode current
76
21
Display
gain
220 nA/2
nA
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Table 7
3
Diagnostic Signals on Analog Output (continued)
Signal
Analog Out
1 V is equal to
Display
Reference diode current
220 nA/2gain
nA
Board Temperature
102.4 °C (displayed value
minus zero offset)
°C
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Service Dialog
Available on both user interfaces.
NO TE
This dialog interface displays the values on the screen only. The analog signal will not be
affected. Some of the signals can be routed to the analog output selectable in the VWD Settings - Analog screen.
On the Control Module it can be accessed from System - Tests - VWD. Then
press the m- (menu) key and select Service.
Selection list of
signal 1 and 2
Actual values of
signal 1 and 2
Position of grating
drive
Figure 16
78
Position of filter
drive
Position grating on
zero-order line
Actual gain setting Actual gain setting
for sample
for reference
Service Dialog
1100 Series Variable Wavelength Detector Reference Manual
Troubleshooting and Test Functions
Table 8
3
Diagnostic Signals
Signal
Display
Analog Output*
Absorbance
AU
1 AU/V
Sample signal without reference
AU
1 AU/V
Reference signal without sample
AU
1 AU/V
Sample raw data
(unfiltered and not logarithmic)
Counts
221 counts/V
Reference raw data
(unfiltered and not logarithmic)
Counts
221 counts/V
Analog output test
Counts
zero offset + 10 µV step
Sample diode current
nA
220 nA/2gain
Reference diode current
nA
220 nA/2gain
Sample preamplifier gain
gain 0 … 5
Reference preamplifier gain
gain 0 … 5
Lamp anode voltage
V
Lamp anode current
mA
Lamp heater voltage
V
Lamp heater current
A
Grating motor steps
Steps
Grating position sensor
ON/OFF
Filter motor position
0 ... 4**
Filter position sensor
ON/OFF
Board Temperature
°C
PTC current
mA
Leak status
-1, 0 … 5***
PTC (leak sensor) voltage
mV
NTC (reference sensor) voltage
V
1100 Series Variable Wavelength Detector Reference Manual
102.4 °C (displayed value
minus zero offset
79
3
Troubleshooting and Test Functions
* for more information see “Diagnostic Signal Output" on page 76
** 0 = in sensor position; 1 = OFF (<370 nm); 2 = cutoff filter in; 3 = unused; 4 = holmium filter in
***-1 = warm-up; 0 = OK; 1 = leak; 2 = NTC short; 3 = NTC open; 4 = PTC short; 5 = PTC open
Signal Descriptions
Constant Zero Offset
This sets the output signal to zero (0 mV).
Absorbance
This is the normal signal (sample plus reference) defined by user wavelength.
Sample signal without reference
This is the normal signal defined by user wavelength without the reference. No
influence from the reference diode.
Reference signal without sample
This is the normal signal defined by user wavelength without the sample. No
influence from the sample diode.
Sample or reference raw data
The sample or reference diode’s data is processed without filtering and
logarithming.
Analog output test
This test adds to the used zero offset setting a DC voltages of 10 µV in cycles of
12 seconds. This 10 µV step is equal to 1 × 10-6 AU and can be used to check
the noise on a recording device. See “DAC Test" on page 71.
Sample or reference diode current
Shows the diodes current.
80
1100 Series Variable Wavelength Detector Reference Manual
3
Troubleshooting and Test Functions
Sample or reference gain
This function shows the current the gain setting.
Lamp anode/heater voltages and currents
Provides the information of actual voltages and currents of the lamp’s anode
and filament.
Grating or Filter Motor motor steps
Provides the step numbers of grating or filter motor settings.
Grating or Filter position sensor
Checks the movement of grating or filter.
Board Temperature
An on-board temperature sensor on the VWM board provides the actual
temperature on the board. This information is running continuously into a
buffer from which it can be retrieved as last-12-hours plot.
Leak Status
Provides status of PTC and NTC sensors. See Table 8 on page 79.
PTC or NTC voltage/current
The actual voltage/current of the PTC (leak sensor) or NTC (reference sensor)
is provided.
1100 Series Variable Wavelength Detector Reference Manual
81
3
82
Troubleshooting and Test Functions
1100 Series Variable Wavelength Detector Reference Manual
Agilent 1100 Series Variable Wavelength Detector
Reference Manual
4
Repairing the Variable Wavelength
Detector
Introduction into Repairing the Variable Wavelength Detector 84
Overview of the Repairing of the Variable Wavelength Detector 87
Simple Repairs 88
Exchanging Internal Parts 102
Agilent Technologies
83
4
Repairing the Variable Wavelength Detector
Introduction into Repairing the Variable Wavelength Detector
Simple Repairs
The detector is designed for easy repair. The most frequent repairs such as
changing the lamp and changing the flow cell can be done from the front of the
detector with the detector in place in the system stack. These repairs are
described in “Simple Repairs" on page 88.
Exchanging Internal Parts
Some repairs may require exchanging defective internal parts. Exchange of
these parts requires removing the detector from the stack, removing the
covers, and disassembling the detector. The security lever at the power input
socket prevents the detector cover from being taken off when line power is still
connected. These repairs are described in “Exchanging Internal Parts" on
page 102.
84
WA RN ING
To prevent personal injury, the power cable must be removed from the detector
before opening the detector cover. Do not connect the power cable to the detector
while the covers are removed.
WA RN ING
When working with solvents please observe appropriate safety procedures (for
example, goggles, safety gloves and protective clothing) as described in the
supplier’s material handling and safety data sheet, especially when using toxic or
hazardous solvents.
CAU TI O N
Electronic boards and components are sensitive to electronic discharge (ESD). In order
to prevent damage always use an ESD protection (for example, the ESD wrist strap
from the accessory kit) when handling electronic boards and components (see “Using
the ESD Strap" on page 86).
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
WA RN ING
4
Eye damage may result from directly viewing the light produced by the deuterium
lamp used in this product. Always turn the deuterium lamp off before removing it.
Cleaning the Detector
The detector case should be kept clean. Cleaning should be done with a soft
cloth slightly dampened with water or a solution of water and a mild
detergent. Do not use an excessively damp cloth allowing liquid to drip into
the spectrometer.
WA RN ING
Do not let liquid drip into the detector. It could cause shock hazard and it could
damage the detector.
1100 Series Variable Wavelength Detector Reference Manual
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4
Repairing the Variable Wavelength Detector
Using the ESD Strap
Electronic boards are sensitive to electronic discharge (ESD). In order to
prevent damage, always use an ESD strap supplied in the standard accessory
kit (see “Accessory Kit" on page 159) when handling electronic boards and
components.
1 Unwrap the first two folds of the band and wrap the exposed adhesive side
firmly around your wrist.
2 Unroll the rest of the band and peel the liner from the copper foil at the
opposite end.
3 Attach the copper foil to a convenient and exposed electrical ground.
Figure 17
86
Using the ESD Strap
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
4
Overview of the Repairing of the Variable Wavelength Detector
Figure 18 below shows the main assemblies and their locations.
VWM board assembly
see page 106
Power supply assembly
see page 124
Interface board,
see page 134
Fan assembly
see page 111
Optical unit assembly
see page 113
Lamp assembly
see page 89
Leak handling
system
see page 101
Flow cell assembly
see page 91 and page 94
Figure 18
Leak sensor
see page 100 and page 122
Main Assemblies
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Repairing the Variable Wavelength Detector
Simple Repairs
On the following pages repairs are described that can be done without opening
the main cover.
Table 9
Simple Repairs
Procedures
Typical Frequency
Notes
Deuterium lamp
exchange
If noise and/or drift exceeds your application limits or
lamp does not ignite.
A VWD test should be performed after
replacement.
Flow cell exchange
If application requires a different flow cell type.
A VWD test should be performed after
replacement.
Cleaning flow cell parts
cleaning or exchange
If leaking or if intensity drops due to contaminated flow
cell windows.
A pressure tightness test should be
done after repair.
Leak sensor drying
If leak has occurred.
Check for leaks.
Leak handling system
replacement
If broken or corroded.
Check for leaks.
88
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Repairing the Variable Wavelength Detector
Exchanging a Lamp
When required
If noise or drift exceeds application limits or lamp does not ignite.
Tools required
Screwdriver POZI 1 PT3
Parts required
Deuterium lamp G1314-60100
NO TE
If you want to use the Agilent 1100 DAD lamp instead of the VWD lamp, you have to change
the lamp settings in the VWD Configuration to lamp type 2140-0590. This ensures that the
DAD lamp’s filament heating is operated like in the DAD. The instrument specifications are
based on the VWD lamp.
WA RN ING
If the detector has been in use, the lamp may be hot. If so, wait five minutes for lamp
to cool down.
Preparations for this procedure:
• Turn the lamp off.
1 Press the release buttons and remove the front cover to
have access to the lamp area.
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Repairing the Variable Wavelength Detector
2 Unscrew the lamp cover and remove it.
3 Unscrew, disconnect and replace the lamp. Insert, fix and
reconnect the lamp.
4 Replace the lamp cover.
5 Replace the front cover.
Next steps:
•
•
•
•
90
Reset the lamp counter as described in the User Interface documentation.
Turn the lamp on.
Give the lamp more than 10 minutes to warm-up.
Perform “Zero-Order Calibration" on page 60 and “656-nm Wavelength Calibration" on page 61 to check the correct
positioning of the lamp.
1100 Series Variable Wavelength Detector Reference Manual
4
Repairing the Variable Wavelength Detector
Exchanging a Flow Cell
When required
If application needs a different type of flow cell or the flow cell needs repair.
Tools required
Two 1/4 inch wrenches for capillary connections
Parts required
Standard Flow Cell G1314-60086 10 mm, 14 µl, 40 bar,
(former Standard Flow Cell G1314-60080 is obsolete)
Micro flow cell, 5 mm, 1 µl, 40 bar, G1314-60081
Semimicro flow cell, 6 mm, 5 µl, 40 bar, G1314-60083
High pressure flow cell, 10 mm, 14 µl, 400 bar, G1314-60082
Preparations for this procedure:
• Turn the lamp off.
1 Press the release buttons and remove the front cover to
have access to the flow cell area.
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4
Repairing the Variable Wavelength Detector
2 Disconnect the inlet and outlet capillaries.
3 Unscrew the thumb screws parallel and remove the flow
cell.
Note:
If you want to maintain flow cell parts, see
“Repairing the Flow Cells" on page 94 or the
information provided with your flow cell.
92
4 Replace the flow cell and fix the thumb screws.
Reconnect the inlet and outlet capillaries to the flow cell.
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
5 Replace the front cover.
4
Next steps:
• To check for leaks, establish a flow and observe the flow
•
•
•
cell (outside of the cell compartment) and all capillary
connections.
Insert the flow cell.
Perform “Zero-Order Calibration" on page 60 and
“656-nm Wavelength Calibration" on page 61 to check
the correct positioning of the flow cell.
Replace the front cover.
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4
Repairing the Variable Wavelength Detector
Repairing the Flow Cells
NO TE
1 - Cell Screw
2 - Conical Springs
3 - Ring #1 PEEK
4 - Gasket #1
5 - Window Quartz
6 - Gasket #2
7 - Cell cover assembly
8 - Ring #2 PEEK
When required
If the flow cell needs repair due to leaks or contaminations.
Tools required
Wrench 1/4 inch for capillary connections
Hexagonal wrench 4 mm
Tooth picks
Parts required
See “Standard Flow Cell (G1314-60086)" on page 142.
See “Standard Flow Cell (G1314-60080)" on page 144
See “Micro Flow Cell" on page 146.
See “Semimicro Flow Cell" on page 148.
See “High Pressure Flow Cell" on page 150.
Preparations
Turn off the flow.
Remove the front cover.
Remove the flow cell, see “Exchanging a Flow Cell" on page 91.
The shown cell parts will differ depending upon the flow cell type. For detailed parts
schematics, refer to above mentioned pages.
1
2
3
5
4
6
5
8
2
1
2
Figure 19
94
Standard Flow Cell
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
Disassembling
the Flow Cell
4
1 Unscrew the cell screw using a 4-mm hexagonal wrench.
2 Remove the SST rings using a pair of tweezers.
3 Use adhesive tape to remove the peek ring, the window and the gasket.
CAU TI O N
Do not use tweezers to remove windows as the surfaces can easily be scratched.
4 Repeat step 1 through step 3 for the other window (keep the parts separate
- otherwise they could be mixed!).
Cleaning the Flow
Cell Parts
5 Pour isopropanol into the cell hole and wipe clean with a piece of lint-free
cloth.
6 Clean the windows with ethanol or methanol. Dry it with a piece of
lint-free cloth.
NO TE
Reassembling the
Flow Cell
NO TE
Always use new gaskets.
7 Hold the flow cell cassette horizontally and place gasket in position.
Ensure both cell holes can be seen through the holes of gasket.
The semimicro #1 and #2 gaskets (items 6 and 7, “Semimicro Flow Cell" on page 149) look
very similar. Do not mix them up.
8 Place the window on gasket.
9 Place the peek ring on the window.
10 Insert the conical springs. Make sure the conical springs point towards the
window. Otherwise tightening the cell screw might break the window.
11 Screw the cell screw into the flow cell and tighten the screw.
12 Repeat the procedure for the other cell side.
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Repairing the Variable Wavelength Detector
Next steps
• Reconnect the capillaries, see “Exchanging a Flow Cell" on page 91.
• Perform a leak test. If OK, insert the flow cell.
• Perform “Zero-Order Calibration" on page 60 and “656-nm Wavelength
Calibration" on page 61 to check the correct positioning of the flow cell.
• Replace the front cover.
96
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Repairing the Variable Wavelength Detector
Using the Cuvette Holder
When required:
If your own standard should be used to checkout the instrument.
Tools required:
None
Parts required:
Cuvette Holder G1314-60200
Cuvette with the “standard”, e.g. NIST certified holmium oxide sample
This cuvette holder can be placed instead of a flow cell in the variable
wavelength detector. Standard cuvettes with standards in it, for example,
National Institute of Standards & Technology (NIST) holmium oxide solution
standard, can be fixed in it.
This can be used for wavelength verifications.
Figure 20
Cuvette Holder
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Repairing the Variable Wavelength Detector
Preparation for this procedure are:
1 Locate the cuvette holder on the desk.
• Remove the normal flow cell.
• Have cuvette with standard available.
2 Unscrew the bracket.
3 Insert the cuvette with the sample into the holder. The
clear side of the cuvette must be visible.
Light path
Clear side
Next steps:
•
•
•
•
98
Reset the lamp counter as described in the User Interface documentation.
Turn the lamp on.
Give the lamp more than 10 minutes to warm-up.
Perform “Zero-Order Calibration" on page 60 and “656-nm Wavelength Calibration" on page 61 to check the correct
positioning of the lamp.
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
4 Replace the bracket and fix the cuvette.
4
5 Install the cuvette holder in the instrument.
Next steps:
• Perform your verification.
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4
Repairing the Variable Wavelength Detector
Correcting Leaks
When required
If a leakage has occurred in the flow cell area or at the capillary connections.
Tools required
Tissue
Two 1/4 inch wrenches for capillary connections
Parts required
None
1 Remove the front cover.
2 Use tissue to dry the leak sensor area.
3 Observe the capillary connections and the flow cell area for leaks and
correct, if required.
4 Replace the front cover.
Leak plane
Waste outlet
Leak sensor assembly
Figure 21
100
Drying the Leak Sensor
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
4
Replacing Leak Handling System Parts
When required
If the parts are corroded or broken.
Tools required
None
Parts required
Leak funnel 5061-3356
Leak funnel holder 5041-8389
Leak tubing (120 mm) 0890-1711
1 Remove the front cover to have access to the leak handling system.
2 Pull the leak funnel out of the leak funnel holder.
3 Pull the leak funnel with the tubing out of its location.
4 Replace the leak funnel and/or the tubing.
5 Insert the leak funnel with the tubing in its position.
6 Insert the leak funnel into the leak funnel holder.
7 Replace the front cover.
Leak funnel holder
Leak funnel
Leak tubing
Leak plan
Waste outlet
Leak sensor assembly
Figure 22
Replacing Waste Handling System Parts
1100 Series Variable Wavelength Detector Reference Manual
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4
Repairing the Variable Wavelength Detector
Exchanging Internal Parts
WA RN ING
The following procedures require opening the main cover of the detector. Always
ensure the detector is disconnected from the line power when the main cover is
removed. The security lever at the power input socket prevents the detector cover
from being taken off when line power is still connected.
WA RN ING
To disconnect the detector from line, unplug the power cord. The power supply still
uses some power, even if the switch on the front panel is turned off.
WA RN ING
When working with solvents please observe appropriate safety procedures (for
example. goggles, safety gloves and protective clothing) as described in the
material handling and safety data sheet by the solvent vendor, especially when
using toxic or hazardous solvents.
NO TE
The electronics of the detector will not allow operation of the detector when the top cover
and the top foam are removed. A safety light switch on the main board will inhibit the
operation of the fan immediately. Voltages for the other electronic components will be
turned off after 30 seconds. The status lamp will light up red and an error will be logged into
the logbook of the user interface. Always operate the detector with the top covers in place.
CAU TI O N
Electronic boards and components are sensitive to electronic discharge (ESD). In order
to prevent damage always use an ESD protection (for example, the ESD wrist strap
from the accessory kit) when handling electronic boards and components, see “Using
the ESD Strap" on page 86
WA RN ING
Eye damage may result from directly viewing the light produced by the Deuterium
lamp used in this product. Always turn off the deuterium lamp before removing it.
102
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4
Repairing the Variable Wavelength Detector
Removing the Top Cover and Top Foams
When required
For all repairs inside the detector.
Tools required
Screwdriver POZI 1 PT3
Parts required
Depends on the work inside and the following procedures.
Preparations for this procedure:
•
•
•
•
Turn off the detector.
Disconnect the power cable.
Disconnect capillaries.
Remove detector from stack and place it on the working
bench.
1 Press the release buttons and remove the front cover.
Unclip and remove the waste funnel assembly.
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Repairing the Variable Wavelength Detector
2 Install the ESD strap. Move the power lock across the
power inlet.
3 If installed, unscrew and remove the interface board.
Place the board on the ESD kit.
ESD strap
4 Lift the cover and slide it towards the rear.
5 Unscrew the screws at the rear of the top plate, slide the
plate towards the front and remove it.
104
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Repairing the Variable Wavelength Detector
6 Carefully remove the top foams from the detector, first
part #1, then part #2.
4
Note:
Do not connect a power plug to module after
removing the top covers.
A safety light switch on the main board will turn off
fan (immediately) and electronic (after 30 seconds)
to avoid the operation with removed covers. An error
will be generated (status lamp lights red) and the
logbook will show an error message.
NO TE
The internal components are now accessible. Exchanging specific components are
described in the following sections.
1100 Series Variable Wavelength Detector Reference Manual
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4
Repairing the Variable Wavelength Detector
Exchanging the Processor Board
When required
If the detector main board is defective or for repair on other assemblies
Tools required
Screwdriver POZI 1 PT3
Hexagonal wrenches 5 mm, 7 mm and 15 mm
Parts required
Detector main board VWM (exchange assembly) G1314-69521
1 Turn off the lamp.
2 Switch off the detector, and disconnect the cables.
3 Remove detector from stack and place it on the working bench.
Analog connector
nuts
Remote
GPIB
Board recess
Figure 23
Board recess
Unscrew Connectors From Board
4 Remove the front cover, top cover and top foam section, see “Removing the
Top Cover and Top Foams" on page 103.
5 Use a 5-mm and 7-mm wrench to unscrew the REMOTE and the GPIB
connector and a 15-mm wrench to unscrew the analog connector nut.
6 Disconnect all connectors from the processor board.
106
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Repairing the Variable Wavelength Detector
When removing connectors, counterhold with one hand on connector J3.
J1/2 -CAN
J3 -Interface board
J5 - Test connector
S1
J12 - RS-232C
J15 - REMOTE
J17 - GPIB
J150 - Power supply
J201 - Grating motor
203 - Fan assembly
J204 - Leak sensor
J205 - Grating position
J207 - Filter motor
J208 - Analog output
J209 - Filter position
J400 -ADC board Sample J203
J401 -ADC Board
Reference
J502 - Deuterium lamp
S1 - Configuration switch
B - Battery
Figure 24
J17
J2
J1
J208
J15
J12
B
J150
J5
J201
J207
J209
J205
NO TE
J3
J502
J204
J400
J401
Location of Connectors on VWM Board
7 Remove the processor board. Place the board on the ESD kit.
NO TE
If you also have to replace other assemblies, continue with that work first.
8 On the new board check the switch setting of address switch S1, see
“Setting the 8-bit Configuration Switch" on page 208.
NO TE
An incorrect switch setting (for example, TEST/BOOT) may cause the module to turn in a
basic mode (yellow or red flashing status light). In such a case turn off the module, re-set
the address switches, and turn on the module again.
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Repairing the Variable Wavelength Detector
9 Install the new processor board and reconnect the connectors. Make sure
that the board is fitted correctly in the board recesses in the holes at the
rear panel.
10 Refit the screws at the REMOTE and GPIB connectors and the nut of the
analog connector.
11 Reinstall the top foam section, top cover and front cover, see “Installing the
Foam and the Top Cover" on page 131.
12 Replace detector into the stack and reconnect the cables.
NO TE
If a new VWM board was installed, update the serial number information of the detector in
the user interface, see procedure below.
13 Check the firmware revision of the module. If the firmware revision is older
than the current firmware revision of the module, update the firmware
using the standard firmware update procedure, see “Replacing the
Detector’s Firmware" on page 135.
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Repairing the Variable Wavelength Detector
Entering the Serial Number using the Control Module
1 Connect the control module to the detector. Turn on the detector.
2 On the control module, press System (F5), then Records (F4). Using the
up/down arrows, make sure that the detector is highlighted.
3 Press FW Update (F5). Now, press the m key. This will display a box which
says Update Enter Serial#.
4 Press Enter. This will display the box labeled Serial#.
5 Letters and numbers are created using the up and down arrows. Into the
box labeled Serial#, enter the 10-character serial number for the detector.
When the 10-character serial number is entered, press Enter to highlight
the complete serial number. Then, press Done (F6).
NO TE
For firmware revisions below A.02.00 it is very important never to press Done if the Serial#
box is blank. In this case, the module can no longer be recognized by either the control
module or the Agilent ChemStation. The main board must then be replaced.
6 Turn the detector off, then on again. The Records screen should display the
correct serial number for this module.
7 If a Agilent ChemStation is also connected, restart the Agilent ChemStation
now as well.
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Repairing the Variable Wavelength Detector
Entering the Serial Number using the Agilent ChemStation
Module serial numbers are entered by typing specific commands on the
command line at the bottom of the main user interface screen.
1 To enter a module serial number, type the following command into the
command line:
print sendmodule$(lvwd, “ser YYYYYYYYYY”)
Where: YYYYYYYYYY is the 10-character serial number of the module in
question.
NO TE
The first two characters are letters, which should be capitalized.
The reply line will respond with RA 0000 SER followed by the module serial
number you just entered.
2 Turn off the detector, then on again. Then, restart the Agilent ChemStation.
If the serial number you have just entered is different than the original
module serial number, you will be given the opportunity to edit the
configure 1100 access screen during the restart of the Agilent ChemStation.
3 After restart, the serial number you have just entered can be seen under the
Instrument menu of the main user interface screen. The serial number of
the detector can also be seen by typing the following command into the
command line:
print sendmodule$ (lvwd, “ser?”)
The reply line will give the module serial number.
110
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Repairing the Variable Wavelength Detector
Exchanging the Fan
CAU TI O N
When required
If the fan is defective or noisy
Tools required
Screwdriver POZI 1 PT3
Parts required
Fan G1314-65004
The fan must be installed in the correct orientation to ensure optimum cooling and
operation of the detector.
Preparations for this procedure:
• Turn off the lamp.
• Switch off the detector, and disconnect the cables.
• Remove detector from stack and place it on the working
1 Disconnect the fan from the main board and remove the
fan assembly.
bench.
• Remove the front cover, top cover and top foam section,
see “Removing the Top Cover and Top Foams" on
page 103.
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Repairing the Variable Wavelength Detector
2 Remove the bumper from the fan.
7 Fit the fan assembly into its location. Replace and
3 Unscrew the fan protection grill.
reconnect the fan assembly to the main board.
4 Exchange the fan.
5 Refit the fan protection grill and tighten the screws using
a screwdriver and 7 mm wrench.
6 Replace the bumpers.
Note:
Next steps:
The fan must be installed in the correct orientation to
ensure optimum cooling and operation of the
detector. The direction of air flow is from the front
towards the rear.
• Reinstall the top foam section, top cover and front
•
•
cover, see “Installing the Foam and the Top Cover" on
page 131.
Replace detector into the stack.
Reconnect the cables and turn on the detector.
Air flow
112
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Repairing the Variable Wavelength Detector
Repairs in the Optical Unit
Inside the optical unit the following assemblies may be replaced by detector
users:
• the source lens assembly, described on page 114, and
• the filter assembly, described on page 117.
All other repairs within the optical unit require special knowledge and
training. Therefore these repairs and calibrations must be performed by
Agilent Technologies trained service engineers.
In case of the following problem symptoms call your local Agilent Technologies
service representative for help:
NO TE
Troubleshooting should be performed with a clean and bubble-free flow cell and with a new
deuterium lamp or one in good condition.
• low intensity readings after replacement of lamp and/or cleaning of the
flow cell and/or source lens assembly,
• calibration failures (zero-order, holmium oxide lines or 656 nm emission
lines),
• excessive noise and/or drift,
• grating drive failures,
• filter drive failures, and
• no intensity readings on one or both, the reference and/or sample side.
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Repairing the Variable Wavelength Detector
Exchanging the Source Lens Assembly
When required
The lens assembly may be replaced when the transmission of light is reduced
and a lamp exchange does not improve the light-throughput
Tools required
Screwdriver POZI 1 PT3
Hexagonal wrench 1.5 mm
Parts required
Source lens assembly G1314-65008
Preparation for this procedure are:
1 Remove the top cover of the optical unit.
• Turn off the lamp.
• Switch off the detector, and disconnect the cables.
• Remove the detector from stack and place it on the
working bench.
• Remove the front cover, top cover and top foam section,
see ““Removing the Top Cover and Top Foams" on
page 103.
114
1100 Series Variable Wavelength Detector Reference Manual
4
Repairing the Variable Wavelength Detector
2 Locate the source lens and filter assembly.
3 Using a tweezers, counterhold the filter assembly.
Unscrew and remove the top part of the filter assembly.
Source lens
assembly
Filter assembly
4 Loosen the setscrew and slide the lens assembly out of
its holder.
Note:
Unscrew the insert and place the assembly as
shown. The lens cannot be removed. It is cleanable
with alcohol from the outside. Do not scratch the
lens nor get contaminations onto it.
1100 Series Variable Wavelength Detector Reference Manual
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4
Repairing the Variable Wavelength Detector
5 Slide the lens assembly into its position. Center the
6 Observe the orientation of the filter assembly top part.
marker on the lens assembly. Fix it the setscrew.
7 Replace the cover of the optical unit.
Install the assembly and fix the screws.
Next steps:
• Replace the bumpers in the top foam so that they are
positioned on the optical unit.
• Reinstall the front cover, top cover and top foam
•
•
•
116
section, see “Installing the Foam and the Top Cover" on
page 131.
Replace the front cover.
Replace the detector into the stack.
Reconnect the cables and capillaries and turn on the
detector.
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
4
Exchanging the Filter Assembly
When required
The filter assembly may be replaced when the transmission of light is reduced
and a lamp or source lens exchange does not improve the light-throughput
Tools required
Screwdriver POZI 1 PT3
Parts required
Filter assembly G1314-65010
Preparation for this procedure are:
1 Remove the top cover of the optical unit.
• Turn off the lamp.
• Switch off the detector, and disconnect the cables.
• Remove the detector from stack and place it on the
working bench.
• Remove the front cover, top cover and top foam section,
see ““Removing the Top Cover and Top Foams" on
page 103.
1100 Series Variable Wavelength Detector Reference Manual
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4
Repairing the Variable Wavelength Detector
2 Locate the source lens and filter assembly.
3 Using a tweezers, counterhold the filter assembly.
Unscrew and remove the top part of the filter assembly.
Source lens
assembly
Filter assembly
Note:
4 Observe the orientation of the filter assembly top part.
The filter may be cleaned with alcohol. Do not
scratch the filter nor get contaminations onto it.
118
Install the assembly and fix the screws.
1100 Series Variable Wavelength Detector Reference Manual
4
Repairing the Variable Wavelength Detector
5 Replace the cover of the optical unit.
Next steps:
• Replace the bumpers in the top foam so that they are
positioned on the optical unit.
• Reinstall the front cover, top cover and top foam
•
•
•
section, see “Installing the Foam and the Top Cover" on
page 131.
Replace the front cover.
Replace the detector into the stack.
Reconnect the cables and capillaries and turn on the
detector.
1100 Series Variable Wavelength Detector Reference Manual
119
4
Repairing the Variable Wavelength Detector
Removing the Optical Unit
When required
When other assemblies have to be removed or when defective
Tools required
Screwdriver POZI 1 PT3
Parts required
Optical unit G1314-69060 (exchange part)
Preparation for this procedure are:
•
•
•
•
•
120
1 Keep the bumpers safe.
Turn off the detector.
Disconnect the power cable.
Disconnect capillaries.
Remove the detector from stack and place it on the
working bench.
Remove the front cover, top cover and top foam section,
see ““Removing the Top Cover and Top Foams" on
page 103.
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
4
2 Remove the lamp cover and the plate above the flow cell.
3 Disconnect the deuterium lamp connector and all cables
coming from the optical unit to the main board.
4 Lift the optical unit out of the foam.
5 Place the optical unit on a bench.
1100 Series Variable Wavelength Detector Reference Manual
121
4
Repairing the Variable Wavelength Detector
Exchanging the Leak Sensor
When required
If defective
Tools required
Screwdriver POZI 1 PT3
Parts required
Leak sensor assembly 5061-3356
1 Turn off the lamp.
2 Switch off the detector, and disconnect the cables and capillaries.
3 Remove the detector from the stack and place it on the working bench.
4 Remove the front cover, top cover and top foam section, see “Removing the
Top Cover and Top Foams" on page 103.
5 Remove the optical unit, see “Removing the Optical Unit" on page 120.
6 Remove the leak base from the cabinet bottom by
unlocking it with a flat blade on the right and left side.
122
7 Disconnect the leak sensor assembly on the processor
board and remove it from the leak panel.
1100 Series Variable Wavelength Detector Reference Manual
4
Repairing the Variable Wavelength Detector
8 Replace the leak sensor assembly into the leak panel and
route the cable through the cabinet and foam channel.
Reconnect it to the main board.
9 Replace the leak base by pressing it into the bottom of the
cabinet.
10 Replace the optical unit, see “Installing the Optical Unit" on page 129.
11 Replace the front cover, top cover and top foam section, see “Installing the
Foam and the Top Cover" on page 131.
12 Replace the detector into the stack.
13 Reconnect the cables and capillaries.
14 Turn on the detector.
1100 Series Variable Wavelength Detector Reference Manual
123
4
Repairing the Variable Wavelength Detector
Exchanging the Power Supply
NO TE
When required
If defective
Tools required
Screwdriver POZI 1 PT3
Wrench 1/4 inch
Wrench 5 mm, 7 mm and 15 mm
Parts required
Power supply 0950-2528
The repair level of the power supply assembly is exchanging the complete assembly. No
serviceable parts are inside.
1 Turn off the lamp.
2 Switch off the detector, and disconnect the cables.
3 Remove the detector from the stack and place it on the working bench.
4 Remove the front cover, top cover and top foam section, see “Removing the
Top Cover and Top Foams" on page 103.
5 Remove the processor board, see “Exchanging the Processor Board" on
page 106.
6 Remove the fan assembly, see “Exchanging the Fan" on page 111.
7 Remove the optical unit, see “Removing the Optical Unit" on page 120.
124
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
8 Carefully remove the bottom foam part by sliding it out
towards the rear.
10 Press down the power switch light pipe to remove it from
the coupler.
4
9 Unscrew the power supply at the rear of the detector.
11 Remove the power supply completely. Reuse the coupler
on the new power supply.
Power
switch light
pipe
Coupler
NO TE
The repair level of the power supply assembly is exchanging of the complete assembly. No
serviceable parts are inside.
1100 Series Variable Wavelength Detector Reference Manual
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4
Repairing the Variable Wavelength Detector
12 Insert the power supply into its location and fix it with the
screw at the rear panel.
13 Press down and clip in the power switch light pipe into the
power supply.
Power
switch light
pipe
14 Reinstall bottom foam part. Slide it in underneath the leak
drain.
15 Route the lamp cable and the leak sensor cable to the
main board.
16 Reinstall the processor board, see “Exchanging the Processor Board" on
page 106.
17 Reinstall the Fan Assembly, see “Exchanging the Fan" on page 111.
126
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
4
18 Reinstall the optical unit, see “Replacing Status Light Pipe" on page 128.
19 Reinstall the front cover, top cover and top foam section, see “Installing the
Foam and the Top Cover" on page 131.
20 Replace the detector into the stack.
21 Reconnect the power cable and turn on the detector.
1100 Series Variable Wavelength Detector Reference Manual
127
4
Repairing the Variable Wavelength Detector
Replacing Status Light Pipe
When required
When part is broken
Tools required
Screwdriver POZI 1 PT3
Parts required
Status light pipe 5041-8384
Preparations for this procedure are:
1 The status light pipe is clipped into the top cover.
• Remove the front cover and top cover, ““Removing the
Top Cover and Top Foams" on page 103.
2 Replace the foam section, the top cover and front cover, see “Installing the Foam and the Top Cover" on page 131.
3 Replace the detector into the stack and reconnect the cables and capillaries.
4 Turn on the detector.
128
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
4
Installing the Optical Unit
When required
To continue the installation
Tools required
Screwdriver POZI 1 PT3
Wrench 1/4 inch
Prerequisites
The optical unit is already removed
All other assemblies are already installed
Preparation for this procedure are:
1 Place the optical unit into the bottom foam.
• The power supply, the fan and the processor board are
already installed.
1100 Series Variable Wavelength Detector Reference Manual
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4
Repairing the Variable Wavelength Detector
2 Reconnect all cables to the main board and the deuterium
lamp connector.
3 Replace the lamp cover and the plate that secures the
optical unit.
4 Replace the bumpers in the top foam so that they are
positioned on the optical unit as shown.
Next steps:
• Reinstall the front cover, top cover and top foam
•
•
130
section, see “Installing the Foam and the Top Cover" on
page 131.
Replace the detector into the stack.
Reconnect the cables and capillaries and turn on the
detector.
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
4
Installing the Foam and the Top Cover
When required
When all repairs have been completed
Tools required
Screwdriver POZI 1 PT3
Prerequisites
The detector is open and other procedures have been carried out
Preparations for this procedure:
1 Carefully fit the top foams into the detector.
• All previous repairs have been completed.
1100 Series Variable Wavelength Detector Reference Manual
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4
Repairing the Variable Wavelength Detector
2 Slide the top plate towards the rear and fix the top plate
3 Replace the cover.
screws.
assure correct
fit with Z-plane
4 If installed, replace the interface board and fix the screws.
5 Replace the waste funnel and the front panel.
Next steps:
• Replace the detector into the stack.
• Reconnect the cables and capillaries and turn on the detector.
132
1100 Series Variable Wavelength Detector Reference Manual
Repairing the Variable Wavelength Detector
4
Assembling the Main Cover
NO TE
WA RN ING
When required
If cover is broken
Tools required
None
Parts required
Plastics kit 5062-8582 (includes base, top, left and right sides)
The plastics kit contains all parts, but it is not assembled.
In case you insert the left or right side in the opposite position, you may not be able
to remove the side from the top part.
1 Place the top part on the bench and insert the left and
2 Replace the cover.
right side into the top part.
Next steps:
• Replace the detector into the stack and reconnect the cables and capillaries.
• Turn on the detector.
1100 Series Variable Wavelength Detector Reference Manual
133
4
Repairing the Variable Wavelength Detector
Replacing the Interface Board
When required
For all repairs inside the detector or for installation of the board
Part required
Interface board (BCD) G1351-68701 with external contacts and BCD outputs
Interface board (LAN), see “Optional Interface Boards" on page 200.
Tools required
None
1 Install the ESD strap. Move the power lock across the
power inlet.
2 If required, unscrew and remove the interface board.
Place the board on the ESD kit.
ESD strap
power lock
3 If required, insert the interface board and fix the screws.
Next steps:
• Remove the ESD strap.
• Reinstall the module into the stack.
134
1100 Series Variable Wavelength Detector Reference Manual
4
Repairing the Variable Wavelength Detector
Replacing the Detector’s Firmware
The installation of new firmware is required:
• if new version solves problems of currently installed version, or
• if after exchange of the processor board (VWM) the version on board is
older than previous installed one.
To upgrade the detector’s firmware the following steps have to be performed:
1 Load the firmware into the detector, see the help system of your user
interface.
NO TE
Update of operation firmware can be done in the resident mode only.
Update of the resident firmware can be done in the normal mode only.
2 Perform a zero-order and a 656-nm calibration to add the calibration
parameters into the board’s memory, see “Wavelength Calibration" on
page 58.
NO TE
The zero-order and a 656-nm calibration should be performed after at least 10 minutes of
warm-up time of the lamp.
3 Enter the serial number information of the detector through the user
interface, see “Entering the Serial Number using the Control Module" on
page 109 or “Entering the Serial Number using the Agilent ChemStation" on
page 110.
1100 Series Variable Wavelength Detector Reference Manual
135
4
136
Repairing the Variable Wavelength Detector
1100 Series Variable Wavelength Detector Reference Manual
Agilent 1100 Series Variable Wavelength Detector
Reference Manual
5
Identifying Parts and Materials
Overview of Main Assemblies 138
Optical Unit and Fan Assembly 140
Source Lens and Filter Assembly 141
Standard Flow Cell (G1314-60086) 142
Standard Flow Cell (G1314-60080) 144
Micro Flow Cell 146
Semimicro Flow Cell 148
High Pressure Flow Cell 150
Cuvette Holder 152
Control Module 153
Sheet Metal Kit 154
Plastic Parts 155
Foam Parts 156
Power and Status Light Pipes 157
Leak Parts 158
Accessory Kit 159
Cable Overview 160
RS-232 Cable Kit 177
LAN Cables 178
Agilent Technologies
137
5
Identifying Parts and Materials
Overview of Main Assemblies
1
3
2
4
6
5
10
14
10
8
9
Figure 25
138
Overview of Main Assemblies
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
Table 10
5
Main Assemblies
Item
Description
Part Number
1
VWD main board VWM (exchange part)
G1314-69521
Cable CAN assembly
5181-1516
Hexagonal nut for GPIB connector
0380-0643
Hexagonal nut for RS-232C connector
1251-7788
Nut for analog connector
2940-0256
Washer for analog connector
2190-0699
2
Power supply assembly, additional power and status light parts, see page 157
0950-2528
3
Interface board BCD/External Contacts
G1351-68701
4
Fan assembly, additional parts, see page 140
G1314-65004
5
Optical unit (exchange part), additional optical unit parts, see page 140
G1314-69060
6
Deuterium lamp
G1314-60100
8
Standard flow cell, 10 mm 14 µl, additional flow cell parts, see page 144
G1314-60080
Micro flow cell, 5 mm 1 µl, additional flow cell parts, see page 146
G1314-60081
High pressure flow cell, 10 mm 14 µl, additional flow cell parts, see page 150
G1314-60082
Semimicro flow cell, 6 mm 5 µl, additional flow cell parts, see page 148
G1314-60083
9
Leak sensor assembly
5061-3356
10
Leak handling parts
see page 158
11
Plastic parts (housing)
see page 155
12
Sheet metal parts
see page 154
13
Foam parts
see page 156
14
Power and status light parts
see page 157
1100 Series Variable Wavelength Detector Reference Manual
139
5
Identifying Parts and Materials
Optical Unit and Fan Assembly
NO TE
Certain repairs in the optical unit require special knowledge, see “Removing the Optical
Unit" on page 120.
Table 11
Optical Unit and Fan Assembly
Item
Description
Part Number
1
Optical unit (exchange part)
G1314-69060
2
Deuterium lamp
G1314-60100
3
Fan assembly
G1314-65004
For lens assembly and filter top assembly, see “Source Lens and
Filter Assembly" on page 141
4
Bumper kit, includes 8 for optical unit plus 4 for fan
G1314-65021
1
3
2
4
Figure 26
140
Optical Unit and Fan Assembly
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
5
Source Lens and Filter Assembly
Table 12
Source Lens and Filter Assembly
Item
Description
Part Number
1
Lens assembly complete
G1314-65008
2
Filter assembly top
G1314-65010
1
2
Figure 27
Source Lens and Filter Assembly
1100 Series Variable Wavelength Detector Reference Manual
141
5
Identifying Parts and Materials
Standard Flow Cell (G1314-60086)
NO TE
This flow cell replaced flow cell G1314-60080, see “Standard Flow Cell (G1314-60080)" on
page 144. The new standard flow cell has been improved on refractive index response.
Table 13
142
Standard Flow Cell G1314-60086
Item
Description
Part Number
1
Cell screw kit, quantity=2
G1314-65062
2
Conical spring kit, quantity=10
79853-29100
3
Ring #1 PEEK kit, quantity=2
G1314-65065
4
Gasket #1, KAPTON, quantity=10
G1314-65063
5
Window quartz kit, quantity=2
79853-68742
6
Gasket #2, KAPTON, quantity=10
G1314-65064
7
Cell cover assembly
G1314-65023
8
Ring #2 PEEK kit, quantity=2
G1314-65066
1100 Series Variable Wavelength Detector Reference Manual
5
Identifying Parts and Materials
1 - Cell Screw
2 - Conical Springs
3 - Ring #1 PEEK
4 - Gasket #1
5 - Window Quartz
6 - Gasket #2
7 - Cell cover assembly
8 - Ring #2 PEEK
1
2
3
5
4
6
5
8
2
1
2
Figure 28
Standard Flow Cell (G1314-60086)
1100 Series Variable Wavelength Detector Reference Manual
143
5
Identifying Parts and Materials
Standard Flow Cell (G1314-60080)
NO TE
The flow cell G1314-60080 is obsolete since May 2001. The repair parts (repair kit,
windows, gaskets, etc. will continue to be available. The cell was replaced by G1314-60086
Table 14
Item
Description
Part Number
Standard flow cell assembly, 10 mm, 14 µl, 40 bar
G1314-60080
(no longer orderable)
Capillary column – detector PEEK 600 mm lg, 0.17 i.d.
5062-8522
Cell screw
79853-27200
Cell kit STD, consists of:
two windows, two gaskets #1 and two gaskets #2.
G1314-65050
2
Conical spring kit, quantity=10
79853-29100
3
Ring SST kit, quantity=2
79853-22500
4
Gasket #1, PTFE, quantity=10
79853-68743
5
Window quartz kit, quantity=2
79853-68742
6
Gasket #2, PTFE, quantity=10
G1314-65051
7
Cell cover assembly
G1314-65023
see note below
1
NO TE
Standard Flow Cell G1314-60080
Early flow cells did not have a cell cover. In case of stray light problems, order cell cover
assembly which includes cell plate, one each of standard, micro and high pressure cell label
and modification procedure.
Figure 30 on page 145 shows the way of adding the cell cover assembly to the flow cells.
144
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
1 - Cell Screw
5
1
2 - Conical Springs
2
3 - Ring SST
3
4
4 - Gasket #1
5
6
5 - Window Quartz
6 - Gasket #2
6
7 - Cell cover assembly
5
4
3
2
1
7
Figure 29
Standard Flow Cell
Figure 30
Modification of Flow Cells with Cell Cover Assembly
1100 Series Variable Wavelength Detector Reference Manual
145
5
Identifying Parts and Materials
Micro Flow Cell
Table 15
Item
Description
Part Number
Micro flow cell, 5 mm, 1 µl, 40 bar
G1314-60081
Capillary column – detector SST 400 mm lg, 0.12 i.d.
5021-1823
Cell screw
79853-27200
Cell kit micro, comprises:
two windows, two gaskets #1 and two gaskets #2
G1314-65052
2
Conical spring kit, quantity=10
79853-29100
3
Ring SST kit, quantity=2
79853-22500
4
Window quartz kit, quantity=2
79853-68742
5
Gasket #1, PTFE, quantity=10
79853-68743
6
Gasket #2, PTFE, quantity=10
G1314-65053
7
Cell cover assembly
G1314-65023
see note below
1
NO TE
Repair Parts: Micro Flow Cell Assembly
Early flow cells did not have a cell cover. In case of stray light problems, order cell cover
assembly which includes cell plate, one each of standard, micro and high pressure cell label
and modification procedure.
Figure 30 on page 145 shows the way of adding the cell cover assembly to the flow cells.
146
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
1 - Cell Screw
5
1
2 - Conical Springs
2
3 - Ring SST
3
4
4 - Gasket #1
5
6
5 - Window Quartz
6 - Gasket #2
6
7 - Cell cover assembly
5
4
3
2
1
7
Figure 31
Micro Flow Cell
1100 Series Variable Wavelength Detector Reference Manual
147
5
Identifying Parts and Materials
Semimicro Flow Cell
Most part number are the same as for the standard flow cell G1314-60080
described on page 144. The new numbers are marked with an asterix (*).
Table 16
Item
Description
Part Number
Semimicro flow cell assembly, 6 mm, 5 µl, 40 bar
G1314-60083
Cell screw
79853-27200
Semimicro cell kit, consisting of:
two windows, two #1 standard gaskets, one #1 semimicro
gasket and one #2 semimicro gasket.
G1314-65056
2
Conical springs, (pack of 10)
79853-29100
3
Ring SST, (pack of 2)
79853-22500
4
PTFE #1 standard gasket, (pack of 10)
79853-68743
5
Quartz window, (pack of 2)
79853-68742
6
Semimicro #1 gasket, PTFE, (pack of 10)
G1314-65057
7
Semimicro #2 gasket, PTFE, (pack of 10)
G1314-65058
8
Cell cover assembly
G1314-65023
Inlet capillary, 400 mm long, 0.12 mm i.d.
5021-1823
1
NO TE
148
Repair Parts: Semimicro Flow Cell Assembly
The semimicro #1 and #2 gaskets (items 6 and 7) look very similar. Do not mix them up.
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
1 - Cell screw
2 - Conical springs
3 - Ring SST
4 - Gasket #1
5 - Quartz window
6 - Semimicro gasket #1
7 - Semimicro gasket #2
8 - Cell cover assembly
5
1
2
3
4
5
7
6
5
4
3
2
8
Figure 32
1
Semimicro Flow Cell
1100 Series Variable Wavelength Detector Reference Manual
149
5
Identifying Parts and Materials
High Pressure Flow Cell
Table 17
Item
Description
Part Number
High pressure flow cell, 10 mm, 14 µl, 400 bar
G1314-60082
Capillary column–detector SST 380 mm lg, 0.17 i.d.
(one side not assembled)
G1315-87311
Cell screw
79853-27200
Cell kit Agilent, comprises:
two windows, two KAPTON gaskets and two PEEK rings
G1314-65054
2
Ring PEEK kit, quantity=2
79853-68739
3
Window quartz kit, quantity=2
79853-68734
4
Gasket kit, KAPTON, quantity=10
G1314-65055
5
Cell cover assembly
G1314-65023
see note below
1
NO TE
Repair Parts: High Pressure Flow Cell Assembly
Early flow cells did not have a cell cover. In case of stray light problems, order Cell Cover
Assembly which includes cell plate, one each of standard, Micro and High Pressure Cell
label and modification procedure.
Figure 30 on page 145 shows the way of adding the cell cover assembly to the flow cells.
150
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
1 - Cell screw
5
1
2 - Ring PEEK
2
3
3 - Window quartz
4
4 - Gasket KAPTON
5 - Cell Cover
4
3
2
1
5
Figure 33
High Pressure Flow Cell
1100 Series Variable Wavelength Detector Reference Manual
151
5
Identifying Parts and Materials
Cuvette Holder
Table 18
Item
Control Module Parts
Description
Part Number
Cuvette Holder
G1314-60200
For informationon the use of the cuvette holder, refer to “Using the Cuvette
Holder" on page 97.
Figure 34
152
Cuvette Holder
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
5
Control Module
Table 19
Item
Control Module Parts
Description
Part Number
Control Module, replacement part including cable
G1323-67001
Plastic Housing Kit, includes front, back and a clamp
5062-8583
CAN cable Agilent 1100 module to control module
G1323-81600
Figure 35
Control Module
1100 Series Variable Wavelength Detector Reference Manual
153
5
Identifying Parts and Materials
Sheet Metal Kit
Table 20
Item
Sheet Metal Kit Parts
Description
Part Number
Sheet metal kit
includes items 1, 2 and 3
G1314-65017
1
Top cover
2
Case
3
Plate
4
Cover for lamp
G1314-65018
1
3
2
4
Figure 36
154
Sheet Metal Kit Parts
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
5
Plastic Parts
Table 21
NO TE
Plastics Parts
Item
Description
Part Number
1
Front cover
5062-8582
2
Plastics, includes base, sides and top
5062-8565
3
Name plate Agilent 1100 Series
5042-1381
For correct assembling of the top and sides, see “Assembling the Main Cover" on page 133.
2
3
1
Figure 37
Plastic Parts
1100 Series Variable Wavelength Detector Reference Manual
155
5
Identifying Parts and Materials
Foam Parts
Table 22
NO TE
Foam Parts
Item
Description
Part Number
1, 2
EPP foam kit, includes
Base and top (part #1 and #2)
G1314-65019
3
Damper kit (includes bumpers for optical and fan)
G1314-65021
4
Guides for interface board
5041-8395
Do not order the individual part numbers mentioned on the foam.
3
2
2
Figure 38
156
Foam Parts
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
5
Power and Status Light Pipes
Table 23
Item
Power and Status Light Pipes
Description
Part Number
Power supply assembly
0950-2528
Screw M4 × 0.7, 8 mm lg, to fix power supply at rear panel
0515-0910
Washer
2190-0409
1
Power light pipe
5041-8382
2
Status light pipe
5041-8384
3
Power switch button
5041-8381
4
Coupler for power supply actuator
5041-8383
4
1
3
2
Figure 39
Power and Status Light Pipes
1100 Series Variable Wavelength Detector Reference Manual
157
5
Identifying Parts and Materials
Leak Parts
Table 24
Leak Parts
Item
Description
Part Number
1
Leak sensor assembly
5061-3356
2
Leak pan
5042-1308
3
Leak funnel
5041-8388
4
Leak funnel holder
5041-8389
5
Clip
5041-8387
6
Corrugated tubing, 120 mm lg, re-order 5 m
5062-2463
7
Corrugated tubing, 1200 mm lg, re-order 5 m
5062-2463
5
2
4
3
1
6
7
Figure 40
158
Leak Parts
1100 Series Variable Wavelength Detector Reference Manual
5
Identifying Parts and Materials
Accessory Kit
This kit contains some accessories and tools needed for the installation and
repair of the detector.
Table 25
Accessory Kit Parts
Description
Part Number
Accessory kit
G1314-68705
Corrugated tubing (to waste), re-order 5 m
5062-2463
Peek outlet capillary kit, i.d. is 0.25 mm (PEEK)
5062-8535
Fitting male PEEK, quantity=1
0100-1516
Hex key 1.5 mm
8710-2393
Hex key 4 mm
8710-2392
Wrench open end 1/4–5/16 inch
8710-0510
Wrench open end 4 mm
8710-1534
ESD wrist strap
9300-1408
1100 Series Variable Wavelength Detector Reference Manual
159
5
Identifying Parts and Materials
Cable Overview
WA RN ING
Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
Table 26
Cables Overview
Type
Description
Part Number
Analog
cables
3390/2/3 integrators
01040-60101
3394/6 integrators
35900-60750
Agilent 35900A A/D converter
35900-60750
General purpose (spade lugs)
01046-60105
3390/2/3 integrators
01040-60101
3390 integrator
01046-60203
3392/3 integrators
01046-60206
3394 integrator
01046-60210
3396A (Series I) integrator
03394-60600
Remote
cables
3396 Series II / 3395A integrator, see page 167
160
3396 Series III / 3395B integrator
03396-61010
Agilent 1100 / 1050 modules / HP 1046A FLD
5061-3378
HP 1046A FLD
5061-3378
Agilent 35900A A/D converter
5061-3378
HP 1040 diode-array detector
01046-60202
HP 1090 liquid chromatographs
01046-60202
Signal distribution module
01046-60202
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
Table 26
5
Cables Overview (continued)
Type
Description
Part Number
BCD
cables
3392/3 integrators
18594-60510
3396 integrator
03396-60560
General purpose (spade lugs)
18594-60520
Auxiliary
Agilent 1100 Series vacuum degasser
G1322-61600
CAN
cables
Agilent 1100 module to module, 0.5 m
5181-1516
Agilent 1100 module to module, 1 m
5181-1519
Agilent 1100 module to control module
G1323-81600
External
contacts
Agilent 1100 Series interface board to general purpose
G1103-61611
GPIB
cable
Agilent 1100 module to Agilent ChemStation, 1 m
10833A
Agilent 1100 module to Agilent ChemStation, 2 m
10833B
RS-232
cable
Agilent 1100 module to a computer
This kit contains a 9-pin female to 9-pin female null
modem (printer) cable and one adapter.
34398A
LAN cable
Twisted pair cross over LAN cable, 10 feet long
(for point to point connection)
5183-4649
Category 5 UTP cable, 8 m long
(for hub connections)
G1530-61480
1100 Series Variable Wavelength Detector Reference Manual
161
5
Identifying Parts and Materials
Analog Cables
One end of these cables provides a BNC connector to be connected to
Agilent 1100 Series modules. The other end depends on the instrument to
which connection is being made.
Agilent 1100 to 3390/2/3 Integrators
Table 27
Connector
01040-60101
Pin
3390/2/3
Pin
Agilent 1100
Signal Name
1
Shield
Ground
2
3
Not connected
Center
4
5
162
Signal +
Connected to pin 6
Shield
Analog -
6
Connected to pin 4
7
Key
8
Not connected
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
5
Agilent 1100 to 3394/6 Integrators
Table 28
Connector
35900-60750
Pin
3394/6
Pin
Agilent 1100
1
Signal Name
Not connected
2
Shield
Analog -
3
Center
Analog +
Pin
BNC
Pin
Agilent 1100
Signal Name
Shield
Shield
Analog -
Center
Center
Analog +
Agilent 1100 to BNC Connector
Table 29
Connector
8120-1840
1100 Series Variable Wavelength Detector Reference Manual
163
5
Identifying Parts and Materials
Agilent 1100 to General Purpose
Table 30
Connector
01046-60105
Pin
3394/6
Pin
Agilent 1100
1
164
Signal Name
Not connected
2
Black
Analog -
3
Red
Analog +
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
5
Remote Cables
One end of these cables provides a Agilent Technologies APG (Analytical
Products Group) remote connector to be connected to Agilent 1100 Series
modules. The other end depends on the instrument to be connected to.
Agilent 1100 to 3390 Integrators
Table 31
Connector
01046-60203
Pin
3390
Pin
Agilent 1100
Signal Name
2
1 - White
Digital ground
NC
2 - Brown
Prepare run
Low
7
3 - Gray
Start
Low
NC
4 - Blue
Shut down
Low
NC
5 - Pink
Not connected
NC
6 - Yellow
Power on
High
NC
7 - Red
Ready
High
NC
8 - Green
Stop
Low
NC
9 - Black
Start request
Low
1100 Series Variable Wavelength Detector Reference Manual
Active
(TTL)
165
5
Identifying Parts and Materials
Agilent 1100 to 3392/3 Integrators
Table 32
Connector
01046-60206
4 - Key
Pin
3392/3
Pin
Agilent 1100
Signal Name
Active
(TTL)
3
1 - White
Digital ground
NC
2 - Brown
Prepare run
Low
11
3 - Gray
Start
Low
NC
4 - Blue
Shut down
Low
NC
5 - Pink
Not connected
NC
6 - Yellow
Power on
High
9
7 - Red
Ready
High
1
8 - Green
Stop
Low
NC
9 - Black
Start request
Low
Pin
3394
Pin
Agilent 1100
Signal Name
Active
(TTL)
9
1 - White
Digital ground
NC
2 - Brown
Prepare run
Low
3
3 - Gray
Start
Low
NC
4 - Blue
Shut down
Low
NC
5 - Pink
Not connected
NC
6 - Yellow
Power on
High
5,14
7 - Red
Ready
High
6
8 - Green
Stop
Low
1
9 - Black
Start request
Low
Agilent 1100 to 3394 Integrators
Table 33
Connector
01046-60210
166
1100 Series Variable Wavelength Detector Reference Manual
5
Identifying Parts and Materials
Table 33
Connector
01046-60210
Pin
3394
Pin
Agilent 1100
13, 15
NO TE
Signal Name
Active
(TTL)
Not connected
START and STOP are connected via diodes to pin 3 of the 3394 connector.
Agilent 1100 to 3396A Integrators
Table 34
Connector
03394-60600
Pin
3394
Pin
Agilent 1100
Signal Name
9
1 - White
Digital ground
NC
2 - Brown
Prepare run
Low
3
3 - Gray
Start
Low
NC
4 - Blue
Shut down
Low
NC
5 - Pink
Not connected
NC
6 - Yellow
Power on
High
5,14
7 - Red
Ready
High
1
8 - Green
Stop
Low
NC
9 - Black
Start request
Low
13, 15
Active
(TTL)
Not connected
Agilent 1100 to 3396 Series II / 3395A Integrators
Use the cable 03394-60600 and cut pin #5 on the integrator side. Otherwise
the integrator prints START; not ready.
1100 Series Variable Wavelength Detector Reference Manual
167
5
Identifying Parts and Materials
Agilent 1100 to 3396 Series III / 3395B Integrators
Table 35
Connector
03396-61010
Pin
33XX
Pin
Agilent 1100
Signal Name
9
1 - White
Digital ground
NC
2 - Brown
Prepare run
Low
3
3 - Gray
Start
Low
NC
4 - Blue
Shut down
Low
NC
5 - Pink
Not connected
NC
6 - Yellow
Power on
High
14
7 - Red
Ready
High
4
8 - Green
Stop
Low
NC
9 - Black
Start request
Low
13, 15
168
Active
(TTL)
Not connected
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
5
Agilent 1100 to HP 1050, HP 1046A or Agilent 35900 A/D Converters
Table 36
Connector
5061-3378
Pin
HP 1050 / …
Pin
Agilent 1100
Signal Name
Active
(TTL)
1 - White
1 - White
Digital ground
2 - Brown
2 - Brown
Prepare run
Low
3 - Gray
3 - Gray
Start
Low
4 - Blue
4 - Blue
Shut down
Low
5 - Pink
5 - Pink
Not connected
6 - Yellow
6 - Yellow
Power on
High
7 - Red
7 - Red
Ready
High
8 - Green
8 - Green
Stop
Low
9 - Black
9 - Black
Start request
Low
Agilent 1100 to HP 1090 LC, HP 1040 DAD or Signal Distribution Module
Table 37
Connector
01046-60202
5 - Key
Pin
HP 1090
Pin
Agilent 1100
Signal Name
1
1 - White
Digital ground
NC
2 - Brown
Prepare run
Low
4
3 - Gray
Start
Low
7
4 - Blue
Shut down
Low
8
5 - Pink
Not connected
NC
6 - Yellow
Power on
High
3
7 - Red
Ready
High
6
8 - Green
Stop
Low
NC
9 - Black
Start request
Low
1100 Series Variable Wavelength Detector Reference Manual
Active
(TTL)
169
5
Identifying Parts and Materials
Agilent 1100 to General Purpose
Table 38
Connector
01046-60201
170
Pin
Universal
Pin
Agilent 1100
Signal Name
Active
(TTL)
1 - White
Digital ground
2 - Brown
Prepare run
Low
3 - Gray
Start
Low
4 - Blue
Shut down
Low
5 - Pink
Not connected
6 - Yellow
Power on
High
7 - Red
Ready
High
8 - Green
Stop
Low
9 - Black
Start request
Low
1100 Series Variable Wavelength Detector Reference Manual
5
Identifying Parts and Materials
BCD Cables
One end of these cables provides a 15-pin BCD connector to be connected to
the Agilent 1100 Series modules. The other end depends on the instrument to
be connected to.
Agilent 1100 to 3392/3 Integrators
Table 39
Connector
18584-60510
6 - Key
Pin
3392/3
Pin
Agilent 1100
Signal Name
BCD Digit
10
1
BCD 5
20
11
2
BCD 7
80
3
3
BCD 6
40
9
4
BCD 4
10
7
5
BCD 0
1
5
6
BCD 3
8
12
7
BCD 2
4
4
8
BCD 1
2
1
9
Digital ground
2
15
+5V
1100 Series Variable Wavelength Detector Reference Manual
Low
171
5
Identifying Parts and Materials
Agilent 1100 to 3396 Integrators
Table 40
Connector
03396-60560
172
Pin
3392/3
Pin
Agilent 1100
Signal Name
BCD Digit
1
1
BCD 5
20
2
2
BCD 7
80
3
3
BCD 6
40
4
4
BCD 4
10
5
5
BCD 0
1
6
6
BCD 3
8
7
7
BCD 2
4
8
8
BCD 1
2
9
9
Digital ground
NC
15
+5V
Low
1100 Series Variable Wavelength Detector Reference Manual
5
Identifying Parts and Materials
Agilent 1100 to General Purpose
Table 41
Connector
18594-60520
Wire Color
Pin
Agilent 1100
Signal Name
BCD Digit
Green
1
BCD 5
20
Violet
2
BCD 7
80
Blue
3
BCD 6
40
Yellow
4
BCD 4
10
Black
5
BCD 0
1
Orange
6
BCD 3
8
Red
7
BCD 2
4
Brown
8
BCD 1
2
Gray
9
Digital ground
White
15
+5 Vt
1100 Series Variable Wavelength Detector Reference Manual
Low
173
5
Identifying Parts and Materials
Auxiliary Cable
One end of this cable provides a modular plug to be connected to the
Agilent 1100 Series vacuum degasser. The other end is for general purpose.
Agilent 1100 Series Degasser to general purposes
Table 42
Connector
G1322-61600
174
Color
Pin
Agilent 1100
Signal Name
White
1
Ground
Brown
2
Pressure signal
Green
3
Yellow
4
Grey
5
DC + 5 V IN
Pink
6
Vent
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
5
CAN Cable
Both ends of this cable provide a modular plug to be connected to Agilent 1100
Series module’s CAN-bus connectors.
Table 43
Agilent 1100 module to module, 0.5 m
5181-1516
Agilent 1100 module to module, 1 m
5181-1519
Agilent 1100 module to control module
G1323-81600
1100 Series Variable Wavelength Detector Reference Manual
175
5
Identifying Parts and Materials
External Contact Cable
5
10
15
1
6
11
One end of this cable provides a 15-pin plug to be connected to Agilent 1100
Series module’s interface board. The other end is for general purpose.
Agilent 1100 Series interface cable to general purposes
Table 44
Connector
G1103-61611
176
Color
Pin
Agilent 1100
Signal Name
White
1
EXT 1
Brown
2
EXT 1
Green
3
EXT 2
Yellow
4
EXT 2
Grey
5
EXT 3
Pink
6
EXT 3
Blue
7
EXT 4
Red
8
EXT 4
Black
9
Not connected
Violet
10
Not connected
Grey/pink
11
Not connected
Red/blue
12
Not connected
White/green
13
Not connected
Brown/green
14
Not connected
White/yellow
156
Not connected
1100 Series Variable Wavelength Detector Reference Manual
Identifying Parts and Materials
5
RS-232 Cable Kit
This kit contains a 9-pin female to 9-pin female null modem (printer) cable and
one adapter. Use the cable and adapter to connect Agilent Technologies
instruments with 9-pin male RS-232 connectors to most PCs or printers.
Agilent 1100 module to PC
RS-232 Cable Kit 34398A
Instrument
DCD
RX
TX
DTR
GND
DSR
RTS
CTS
RI
DB9
Male
PC
1
2
3
4
5
6
7
8
9
DB9
Female
1100 Series Variable Wavelength Detector Reference Manual
1
2
3
4
5
6
7
8
9
DB9
Female
DCD
RX
TX
DTR
GND
DSR
RTS
CTS
RI
DB9
Male
177
5
Identifying Parts and Materials
LAN Cables
Recommended Cables
For point to point connection (not using a network hub) use a twisted pair
cross over LAN cable (P/N 5183-4649, 10 feet long).
For standard network connections using a hub use category 5 UTP cables,
(P/N G1530-61480, 8 m long).
178
1100 Series Variable Wavelength Detector Reference Manual
Agilent 1100 Series Variable Wavelength Detector
Reference Manual
6
Introduction to the Variable Wavelength
Dector
Introduction to the Detector 180
Optical System Overview 181
Electrical Connections 186
Instrument Layout 188
Early Maintenance Feedback (EMF) 189
The Electronics 191
Detector Main Board (VWM) 192
Firmware Description 197
Optional Interface Boards 200
Agilent 1100 Series Interfaces 203
Setting the 8-bit Configuration Switch 208
The Main Power Supply Assembly 213
Agilent Technologies
179
6
Introduction to the Variable Wavelength Dector
Introduction to the Detector
The Agilent 1100 Series variable wavelength detector is designed for highest
optical performance, GLP compliance and easy maintenance with:
• deuterium lamp for highest intensity and lowest detection limit over a
wavelength range of 190 to 600 nm,
• optional flow-cell cartridges (standard 10 mm 14 µl, high pressure 10 mm
14 µl, micro 5 mm 1 µl, semimicro 6 mm 5 µl) are available and can be used
depending on the application needs,
• easy front access to lamp and flow cell for fast replacement, and
• built-in holmium oxide filter for fast wavelength accuracy verification.
For specifications “Performance Specifications" on page 242.
180
1100 Series Variable Wavelength Detector Reference Manual
6
Introduction to the Variable Wavelength Dector
Optical System Overview
Optical System
The optical system of the detector is shown in Figure 41. Its radiation source
is a deuterium-arc discharge lamp for the ultraviolet (UV) wavelength range.
The light beam from the deuterium lamp passes through a lens, a filter
assembly (in position none, cut-off or holmium oxide), an entrance slit, a
spherical mirror (M1), a grating, a second spherical mirror (M2), a beam
splitter, and finally through a flow cell to the sample diode. The beam through
the flow cell is absorbed depending on the solutions in the cell, in which UV
absorption takes place, and the intensity is converted to an electrical signal by
means of the sample photodiode. Part of the light is directed to the reference
photodiode by the beam splitter to obtain a reference signal for compensation
of intensity fluctuation of the light source. A slit in front of the reference
photodiode cuts out light of the sample bandwidth. Wavelength selection is
made by rotating the grating, which is driven directly by a stepper motor. This
configuration allows fast change of the wavelength. The cutoff filter is moved
into the lightpath above 370 nm to reduce higher order light.
Deuterium lamp
Filter assembly
Entrance slit
Lens
Mirror M1
Sample diode
Flow cell
Grating
Beam splitter
Mirror M2
Reference diode
Figure 41
Optical Path of the Variable Wavelength Detector
1100 Series Variable Wavelength Detector Reference Manual
181
6
Introduction to the Variable Wavelength Dector
The Optical Components
Entrance slit assembly
Grating assembly
Filter assembly
Beam splitter assembly
Source lens
assembly
Mirror #1
Lamp
Mirror #2
Photo diode
and ADC
SAMP
ADC REF
Photo diode REF
Flow cell assembly
Figure 42
Mechanical Layout of the Optical Unit
Lamp
The light source for the UV wavelength range is a deuterium lamp. As a result
of plasma discharge in a low pressure deuterium gas, the lamp emits light over
the 190 to 600 nm wavelength range.
Source Lens
Assembly
The source lens receives the light from the deuterium lamp and focuses it onto
the entrance slit.
Entrance Slit
Assembly
The entrance slit assembly has an exchangeable slit. The standard one has a
1-mm slit. For replacement and calibration purposes to optimize the
alignment, a slit with a hole is needed.
Filter Assembly
The filter assembly is electromechanically actuated. During wavelength
calibrations it moves into the lightpath.
The filter assembly has two filters installed and is processor-controlled.
OPENnothing in lightpath
182
1100 Series Variable Wavelength Detector Reference Manual
6
Introduction to the Variable Wavelength Dector
CUTOFFcut off filter in lightpath at λ > 370 nm
HOLMIUMholmium oxide filter for wavelength check.
A photo sensor determines the correct position.
Cutoff filter
Figure 43
Holmium oxide filter
with cutoff filter
Filter Assembly
Mirror Assemblies M1 and M2
The instrument contains two spherical mirrors (M1 and M2). The beam
adjustable is vertically and horizontally. Both mirrors are identical.
NO TE
The mirrors are coated with magnesium fluoride. They should not be touched or cleaned.
This will destroy the surface and reduce the light reflection.
Grating Assembly
The grating separates the light beam into all its component wavelengths and
reflects the light onto mirror #2.
The grating has 1200 lines/mm and is directly rotated by the grating drive
stepper motor, depending on the wavelength entry. The whole range
(190 to 600 nm) is equal to 1550 steps (15.5 °). The stepper motor is
controlled and driven by the detector main board (VWM). The step angle of the
stepper motor rotation is electronically reduced by 0.01 ° per step. This
system has no mechanical reduction mechanism, accordingly the grating is
mounted directly onto the motor shaft.
The stepper motor reference position is determined by a plate fitted onto the
motor shaft, interrupting the beam of a photo sensor. The wavelength
calibration of the grating is done at the zero order light position and at
656 nm, which is the emission line of the deuterium lamp.
1100 Series Variable Wavelength Detector Reference Manual
183
6
Introduction to the Variable Wavelength Dector
NO TE
The grating surface should not be touched or cleaned. This will destroy the surface and
reduce the light reflection.
Beam Splitter Assembly
The beam splitter splits the light beam. One part goes directly to the sample
diode. The other part of the light beam goes to the reference diode.
Photo Diodes Assemblies
Two photodiode assemblies are installed in the optical unit. The sample diode
assembly is located on the left side of the optical unit. The reference diode
assembly is located in the front of the optical unit.
Photo Diode ADC (analog-to-digital converter)
The photo diode current is directly converted to 20-bit digital data direct
photocurrent digitization. The data is transferred to the detector main board
(VWM). The photo diode ADC boards (VWAs) are located close to the photo
diodes.
Flow Cell Assembly
A variety of flow-cell cartridges can be inserted using the same quick and
simple mounting system.
Figure 44
184
Cartridge Type Flow Cell
1100 Series Variable Wavelength Detector Reference Manual
Introduction to the Variable Wavelength Dector
Table 45
6
Flow Cell Data
STD
Semimicro
Agilent
Micro
Maximum pressure
40 (4)
40 (4)
400 (40)
40 (4)
bar (MPa)
Path length
10 (conical)
6 (conical)
10 (conical)
5
mm
Volume
14
5
14
1
µl
Inlet i.d.
0.17
0.17
0.17
0.1
mm
Inlet length
750
750
750
555
mm
Outlet i.d.
0.25
0.25
0.25
0.25
mm
Outlet length
120
120
120
120
mm
Materials in contact with
solvent
SST, quartz,
PTFE, PEEK
SST, quartz,
PTFE
SST, quartz,
Kapton
SST, quartz,
PTFE
1100 Series Variable Wavelength Detector Reference Manual
185
6
Introduction to the Variable Wavelength Dector
Electrical Connections
• The GPIB connector is used to connect the detector to the with a computer.
The address and control switch module next to the
GPIB connector determines the GPIB address of your detector. The
switches are preset to a default address (Table 49 on page 204 or Table 53
on page 209) and is recognized once after power is switched on.
• The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal Agilent 1100 Series
module data transfer and synchronization.
• One analog output provides signals for integrators or data handling
systems.
• The interface board slot is used for external contacts and BCD bottle
number output or LAN connections.
• The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
• With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch
next to the GPIB connector (see “Communication Settings for RS-232C
Communication" on page 210). See your software documentation for
further information.
• The power input socket accepts a line voltage of 100–120 or 220–240 volts
AC ± 10% with a line frequency of 50 or 60 Hz. Maximum power
consumption is 220 VA. There is no voltage selector on your module because
the power supply has wide-ranging capability. There are no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply. The security lever at the power input socket prevents the
module cover from being taken off when line power is still connected.
WA RN ING
186
Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
1100 Series Variable Wavelength Detector Reference Manual
Introduction to the Variable Wavelength Dector
6
Security lever
Slot for interface
board
Analog Signals
APG Remote
RS-232C
CAN
GPIB
Power
Configuration switch
Figure 45
Electrical Connections
1100 Series Variable Wavelength Detector Reference Manual
187
6
Introduction to the Variable Wavelength Dector
Instrument Layout
The industrial design of the module incorporates several innovative features.
It uses Agilent’s E-PAC concept for the packaging of electronics and
mechanical assemblies. This concept is based upon the use of expanded
polypropylene (EPP) layers foam plastic spacers in which the mechanical and
electronic boards components of the module are placed. This pack is then
housed in a metal inner cabinet which is enclosed by a plastic external
cabinet. The advantages of this packaging technology are:
• virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
• the plastic layers have air channels molded into them so that cooling air can
be guided exactly to the required locations,
• the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
• the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.
188
1100 Series Variable Wavelength Detector Reference Manual
Introduction to the Variable Wavelength Dector
6
Early Maintenance Feedback (EMF)
Maintenance requires the exchange of components which are subject to wear
or stress. Ideally, the frequency at which components are exchanged should be
based on the intensity of usage of the instrument and the analytical
conditions, and not on a predefined time interval. The early maintenance
feedback (EMF) feature monitors the usage of specific components in the
instrument, and provides feedback when the user-selectable limits have been
exceeded. The visual feedback in the user interface provides an indication that
maintenance procedures should be scheduled.
EMF Counter
The detector module provides a EMF counter for the lamp. The counter
increments with lamp use, and can be assigned a maximum limit which
provides visual feedback in the user interface when the limit is exceeded. The
counter can be reset to zero after the lamp is exchanged. The detector provides
the following EMF counters:
• Deuterium Lamp On-Time
Using the EMF Counters
The user-settable EMF limits for the EMF counter enables the early
maintenance feedback to be adapted to specific user requirements. The useful
lamp burn time is dependent on the requirements for the analysis (high or low
sensitivity analysis, wavelength, and so on), therefore, the definition of the
maximum limits need to be determined based on the specific operating
conditions of the instrument.
Setting the EMF Limits
The setting of the EMF limits must be optimized over one or two maintenance
cycles. Initially, no EMF limit should be set. When instrument performance
indicates maintenance is necessary, take note of the values displayed by lamp
counters. Enter these values (or values slightly less than the displayed values)
1100 Series Variable Wavelength Detector Reference Manual
189
6
Introduction to the Variable Wavelength Dector
as EMF limits, and then reset the EMF counters to zero. The next time the EMF
counters exceed the new EMF limits, the EMF flag will be displayed, providing
a reminder that maintenance needs to be scheduled.
190
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The Electronics
The electronics are comprised of four main components:
• detector main board (VWM), see page 192.
• power supply, see page 213.
Optional:
• interface board (BCD/external contacts), see page 200.
• interface board (LAN), see page 202.
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Introduction to the Variable Wavelength Dector
Detector Main Board (VWM)
This board controls all information and activities of all assemblies within the
detector module. The operator enters parameters, changes modes and controls
the module, through interfaces (CAN, GPIB or RS-232C), connected to the user
interfaces
ASIC — Application Specific Integrated Circuit
The 304-pin application-specific integrated circuit (ASIC) provides interfacing
to external devices through drivers, including GPIB, CAN, APG remote, and
RS-232C. It is directly connected to the four control LEDs located near the
connectors on this board and the 8-bit configuration switch which is used to
configure the address for the GPIB communication, Baud rate for RS-232C
transfer and so on. For switch settings, Table 53 on page 209.
In addition, it controls the cooling fan through the PWM (pulse width
modulation) driver. Movement of the cooling fan is sensed by the hardware
and the microprocessor.
Mainprocessor
The Motorola MC 68332 microprocessor acts as mainprocessor with a clock
frequency of 16 MHz and exchanges data with the ASIC through the core bus
as well as with the PSRAM (pseudo-static random access memory) of
2 × 512 KB, flash EEPROM (electrically erasable programmable read only
memory) of 1 MB and 1 × 32 KB battery-backed NV-RAM (non-valuable).
Battery life is laid out to last for more than 25 years under normal operating
conditions.
FPGA (Field Programmable Gate Array)
Almost all control and communication of the ADC boards (VWA) and digital
section of grating motor driver is programmed in FPGA.
Firmware
For an outline of the firmware, see “Firmware Description" on page 197.
192
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Leak Converter
This block consists of a PTC (resistor with positive temperature coefficient)
for the leak identification and a NTC (resistor with negative temperature
coefficient) for the ambient temperature measurement. This assures that
temperature changes are not identified as a leak. A leak would cool down the
PTC and its change in resistance would produce a leak signal. The signals are
converted by the diagnostic A/D converter.
Fan Drive
The operation of the fan is controlled by the main processor and runs with
constant revolution. The fan produces a sense signal which is derived from the
revolution. This sense signal is used for diagnostics. If the fan does not turn,
the deuterium lamp and the grating drive are switched off.
Filter Motor Driver
The filter motor driver, controlled by the main processor, drives the filter
motor with constant voltage, 2-phase full stepping.
Grating Motor Driver
The grating motor driver, controlled by the main processor, drives the grating
motor. The motor driver controls and balances each coil current of the
2-phase stepper motor (corrected micro-step stepper motor driver circuit).
The step angle of the stepper motor rotation is reduced electronically into
0.01∞per step. The data of current control for detent torque correction is
programmed on ROM (Read Only Memory).
On-board Battery
An on-board lithium battery buffers the electronic memories when the module
is turned off.
For safety information on lithium batteries “Lithium Batteries Information" on
page 249.
Interfaces
For detailed information on interfaces, see “Agilent 1100 Series Interfaces" on
page 203.
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Status
LEDs
Main Control
Functional Block Diagram
4 control
LEDs
8-bit
switch
GPIB
driver
CAN
driver
VWM
Recorder out
DAC
ASIC
Analog
signal
Fan
Fan driver
application
specific
integrated
circuit
sense
MPS
main
power
supply
Memory
REMOTE
driver
Interface
board
RS-232C
driver
Safety
lock
Real time
clock
Lamp
supply
Main processor
FPGA
field
programmable
gate array
Diagnostic
diagnostic data
ADC
Leak
sensor
Filter motor
driver
Filter
Motor
Grating motor
driver
Grating
motor
serial bus
Regulator
Figure 46
194
Deuteriu
m lamp
VWA
ADC SMP
VWA
ADC REF
Block Diagram VWD Main Controller Functionality
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Introduction to the Variable Wavelength Dector
Deuterium Lamp Filament Control
The deuterium lamp filament control circuit provides a constant voltage of
2.5 VDC at approximately 6 A to the filament of the deuterium lamp. The
deuterium lamp filament control circuit is enabled by the processor.
Depending on the lamp type the heater voltage is turned off or kept lower after
lamp ignition.
Deuterium Lamp Current Control
The deuterium lamp current control circuit comprises two parts. One part
generates an ignition pulse of 600 VDC for the lamp, resulting in lamp ignition.
After ignition this voltage is disabled. The other part is a constant current
source of 320 mA at an operating voltage of about 85 VDC for stable operating
conditions and light emission of the deuterium lamp.
Igniting the Deuterium Lamp
The deuterium lamp filament is heated for several seconds prior to ignition.
The deuterium lamp current control circuit gives an ignition pulse to the lamp,
resulting in lamp ignition. The filament control circuit disables or lowers the
filament voltage (depending on the lamp type) if the lamp was ignited
successfully.
If the deuterium lamp failed to ignite, the whole sequence is repeated after a
wait sequence for cooling down. If the deuterium lamp still does not ignite, an
error message occurs.
Diagnostic A/D Converter
The diagnostic A/D converter senses currents and voltages of the deuterium
and tungsten lamps and converts the analog signals into digital values. The
digital values are transferred via the control bus on the VWM board. When
values are outside of the normal range, an appropriate error message is
generated.
Safety Switches
If the rear EPP foam is removed while the instrument is still on, the safety
light switches are activated and the deuterium lamp, the grating drive and the
fan are switched off.
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Optical Unit
Main Controller Board (VWM)
Main Power Supply (MPS)
+ /-5 V
ADC
Sample
ADC
Reference
J400 + 5 V
Regulator ± 15 V
+36 V
AC Input
+ /-5 V
J401
Grating
Motor
J201
Grating Pos
Sensor
J205
Filter Motor
J207
Filter Pos
Sensor
J209
+24 V
+5V
+24 V
± 15 V
+5V
+5 V
J150
+5V
+5V
J3
150 V/ 600 V
D2 lamp
J502 1.7 V or supply
2.5 V
0 V (GND)
Leak Sensor
J204
Interface
Board
+5 V
+5 V
±-15 V
RS 232C
+24 V
CAN
±12 V
+22 V
J203
+24 V
± 15 V
Figure 47
196
J17
RS-232C
J12
CAN
J1
CAN
J2
+15 V
+5 V
Fan
GPIB
Rec DAC
0...1 V
APG
Remote
Analog Out
J15
J208
Power Interconnection Diagram of the Detector
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Introduction to the Variable Wavelength Dector
Firmware Description
The firmware of the instrument consists of two independent sections:
• a non-instrument specific section, called resident system,
• an instrument specific section, called main system.
Resident System
This resident section of the firmware is identical in all Agilent 1100 series
modules. Its properties are:
• the complete communication capabilities (GPIB, CAN, LAN and RS-232C),
• memory management, and
• ability to update the firmware of the main system.
Main System
Its properties are:
• the complete communication capabilities (GPIB, CAN, LAN and RS-232C),
• memory management, and
• ability to update the firmware of the resident system.
In addition the main system comprises the instrument functions that are
divided into common functions like
• run synchronisation through APG remote
• error handling,
• diagnostic functions and so on,
or module specific functions like
• internal events such as lamp control, grating and filter movements,
• raw data collection and conversion to absorbance (see “Raw Data
Conversion to Absorbance" on page 199, and so on.
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Firmware Updates
Firmware updates can be done using your user interface:
• handheld control module with files from a PC-card, or
• Agilent ChemStation with files from floppy disk.
The file naming conventions are:
xxxx-vvv.DLB, where
xxxxis the product number, e.g. 1314 for the G1314A VWD), and
vvvis the revision number, for example 108 is revision 1.08
For instructions refer to your user interface.
NO TE
Update of main system can be done in the resident system only.
Update of the resident system can be done in the main system only.
Main FW update
Resident System
Main System
Resident FW update
Figure 48
198
Firmware Update Mechanism
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Introduction to the Variable Wavelength Dector
6
Raw Data Conversion to Absorbance
The raw data flow (from the photovariable wavelength) and conversion to
absorbance spectra with variance for each data point is a multiple step
process. This process is outlined in Figure 49.
Raw data
Filtering
Absorbance
calculation
Signal
calculation
Deuterium
line data
Wavelength
calibration
table
Wavelength
control
Figure 49
LC signals
Firmware Flow Diagram
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Introduction to the Variable Wavelength Dector
Optional Interface Boards
The Agilent 1100 Series modules have one optional board slot that allows to
add an interface board to the modules.
Table 46
Optional Interface Boards
Description
Part Number
BCD Board
G1351-68701
Fuse 250 mA (four are on the board)
2110-0004
LAN Board (see next page for details)
200
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Introduction to the Variable Wavelength Dector
BCD Board
The BCD board provides a BCD output for the bottle number of the
Agilent 1100 Series autosampler and four external contacts. The external
contact closure contacts are relay contacts. The maximum settings are: 30 V
(AC/DC); 250 mA (fused). There are general purpose cables available to
connect the BCD output, see “BCD Cables" on page 171 and the external
outputs, see “External Contact Cable" on page 176 to external devices.
RFI filter
Board
identification
Processor
interface
12
BCD
register
BCD
connector
+
External
contacts
Figure 50
250 mA
4x
RFI
filter
Line driver
External
contact
connector
Block Diagram BCD Board
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Introduction to the Variable Wavelength Dector
LAN Board
The HP JetDirect cards are network interface cards used in HP printers.
NO TE
One board is required per Agilent 1100 stack. It is recommended to add the LAN board to
the detector with highest data rate.
NO TE
The LAN board can only be used together with:
a main board version G13XX-66520 (DAD/MWD/VWD/Pump/ALS) or G13XX-66500
(FLD/RID) and above.
a DOS-ChemStation software revision A.06.01 or above.
The following cards can be used with the Agilent 1100 modules.
Table 47
NO TE
LAN Boards
Agilent Order Number
Supported networks
J4106A
Ethernet/802.3, RJ-45 (10Base-T(
J4105A
Token Ring/802.5, DB9, RJ-45 (10Base-T)
J4100A
Fast Ethernet, Ethernet/802.3, RJ-45 (10/100Base-TX) + BNC
(10Base2)
Minimum firmware of the JetDirect cards is A.05.05.
Recommended Cables
For point to point connection (not using a network hub) use a twisted pair
cross over LAN cable (P/N 5183-4649, 10 feet long).
For standard network connections using a hub use category 5 UTP cables,
(P/N G1530-61480, 8 m long).
202
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Agilent 1100 Series Interfaces
The Agilent 1100 Series modules provide the following interfaces:
Table 48
Agilent 1100 Series Interfaces
Interface Type
Pumps
Autosample
r
DA Detector
MW Detector
FL Detector
VW Detector
RI Detector
Thermostatted
Column
Compartment
Vacuum
Degasser
CAN
Yes
Yes
Yes
Yes
Yes
No
GPIB
Yes
Yes
Yes
Yes
Yes
No
RS-232C
Yes
Yes
Yes
Yes
Yes
No
Remote
Yes
Yes
Yes
Yes
Yes
Yes
Analog
Yes
No
2×
1×
No
Yes*
Interface board
Yes
Yes
Yes
Yes
No
No
* The vacuum degasser will have a special connector for specific use. For details see description of main board.
• CAN connectors as interface to other Agilent 1100 Series modules,
• GPIB connector as interface to the Agilent ChemStation,
• RS-232C as interface to a computer,
• REMOTE connector as interface to other Agilent products,
• analog output connector(s) for signal output, and
• interface slot for specific interfacing (external contacts, BCD, LAN and so
on).
For identification and location of the connectors, see Figure 4 on page 20.
WA RN ING
Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations, see “Cable
Overview" on page 160.
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Introduction to the Variable Wavelength Dector
Analog Signal Output
The analog signal output can be distributed to a recording device. For details
refer to the description of the main board of the module.
GPIB Interface
The GPIB connector is used to connect the module with a computer. The
address and control switches next to the GPIB connector determine the GPIB
address of your module. The switches are preset to a default address and
recognized by the operating software from Agilent Technologies.
Table 49
Default Addresses
Autosampler
28
Autosampler
28
Pump
22
RID
29
FLD
23
VWD
24
Autosampler (HP 1050)
18
Agilent 8453A
25
Pump (HP 1050)
16
DAD/MWD
26
VWD (HP 1050)
10
Column Compartment
27
DAD (HP 1050)
17
CAN Interface
The CAN is intermodule communication interface. It is a 2-wire serial bus
system supporting high speed data communication and real-time requirement.
204
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Remote Interface
The APG Remote connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features as common
shut down, prepare, and so on.
Remote control allows easy connection between single instruments or systems
to ensure coordinated analysis with simple coupling requirements.
The subminiature D connector is used. The module provides one remote
connector which is inputs/outputs (wired-or technique).
To provide maximum safety within a distributed analysis system, one line is
dedicated to SHUT DOWN the system’s critical parts in case any module
detects a serious problem. To detect whether all participating modules are
switched on or properly powered, one line is defined to summarize the
POWER ON state of all connected modules. Control of analysis is maintained
by signal readiness READY for next analysis, followed by START of run and
optional STOP of run triggered on the respective lines. In addition PREPARE
and START REQUEST may be issued. The signal level are defined as:
• standard TTL levels (0 V is logic true, + 5 V is false)
• fan-out is 10,
• input load is 2.2 kOhm against + 5 V, and
• output are open collector type, inputs/outputs (wired-or technique).
Table 50
Remote Signal Distribution
Pin
Signal
Description
1
DGND
Digital ground
2
PREPARE
(L) Request to prepare for analysis (for example, calibration,
detector lamp on). Receiver is any module performing preanalysis
activities.
3
START
(L) Request to start run / timetable. Receiver is any module
performing run-time controlled activities.
4
SHUT DOWN
(L) System has serious problem (for example, leak: stops pump).
Receiver is any module capable to reduce safety risk.
5
Not used
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Introduction to the Variable Wavelength Dector
Table 50
Remote Signal Distribution (continued)
Pin
Signal
Description
6
POWER ON
(H) All modules connected to system are switched on. Receiver is
any module relying on operation of others.
7
READY
(H) System is ready for next analysis. Receiver is any sequence
controller.
8
STOP
(L) Request to reach system ready state as soon as possible (for
example, stop run, abort or finish and stop injection). Receiver is any
module performing run-time controlled activities.
9
START REQUEST
(L) Request to start injection cycle (for example, by start key on any
module). Receiver is the autosampler.
RS-232C
The RS-232C connector is used to control the module from a computer
through RS-232C connection, using the appropriate software. This connector
can be configured with the configuration switch module next to the GPIB
connector.
The RS-232C is designed as DCE (data communication equipment) with a
9-pin male SUB-D type connector. The pins are defined as:
Table 51
RS-232C Connection Table
Pin
Direction
Function
1
In
DCD
2
In
RxD
3
Out
TxD
4
Out
DTR
5
206
Ground
6
In
DSR
7
Out
RTS
8
In
CTS
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Introduction to the Variable Wavelength Dector
Table 51
RS-232C Connection Table (continued)
Pin
Direction
Function
9
In
RI
Instrument
PC
DCD
RX
TX
DTR
GND
DSR
RTS
CTS
RI
DB9
Male
Figure 51
6
1
2
3
4
5
6
7
8
9
DB9
Female
1
2
3
4
5
6
7
8
9
DB9
Female
DCD
RX
TX
DTR
GND
DSR
RTS
CTS
RI
DB9
Male
RS-232 Cable
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Introduction to the Variable Wavelength Dector
Setting the 8-bit Configuration Switch
The 8-bit configuration switch is located next to the GPIB connector. Switch
settings provide configuration parameters for GPIB address, serial
communication protocol and instrument specific initialization procedures.
Factory setting is
shown for the variable
wavelength detector
Figure 52
Table 52
8-bit Configuration Switch
8-bit Configuration Switch
Mode Select
1
2
3
GPIB
0
0
RS-232C
0
1
Baud rate
Reserved
1
0
Reserved
TEST/BOOT
1
1
RSVD
4
5
6
7
Data
bits
Parity
RSVD
RSVD
8
GPIB address
SYS
FC
Switches 1 and 2 define which set of parameters (for example, for GPIB,
RS-232C, and so on) will be changed. Once the change has been completed, the
instrument must be powered up again in order to store the values in the
non-volatile memory.
208
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Introduction to the Variable Wavelength Dector
In the non-volatile memory, the parameters are kept, regardless of whether
you turn the instrument off and on again. They will be kept until the same set
of parameters is changed and the power is reset. All other previously stored
configuration settings will still remain in the non-volatile memory.
In this way, you can store more than one set of parameters using the same
8-bit configuration switch twice, for example, for both GPIB and RS-232C.
GPIB Default Addresses
If you just want to change the GPIB address and need a detailed procedure,
refer to the Installing Your Agilent ChemStation System handbook.
Default GPIB address is set to the following addresses:
Table 53
Default Addresses for Agilent Series 1100 Modules
Module
Address
Binary Address
Pump
22
00010110
FLD
23
00010111
VWD
24
00011000
Agilent 8453A
25
00011101
DAD/MWD
26
00011010
Column compartment
27
00011011
Autosampler
28
00011100
RID
29
00011101
where 0 means that the switch is down and 1 means that the switch is up.
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Introduction to the Variable Wavelength Dector
Communication Settings for RS-232C Communication
The communication protocol used in this instrument supports only hardware
handshake (CTS/RTS).
Switches 1 in down and 2 in up position define that the RS-232C parameters
will be changed. Once the change has been completed, the instrument must be
powered up again in order to store the values in the non-volatile memory.
Table 54
Communication Settings for RS-232C Communication
Mode Select
1
2
3
RS-232C
0
1
Baud rate
4
5
6
7
Data
Bits
Parity
8
Use the following tables for selecting the setting which you want to use for
RS-232C communication. The number 0 means that the switch is down and 1
means that the switch is up.
Table 55
Baud Rate Settings
Switches
3
4
5
0
0
0
0
0
0
0
Table 56
210
Baud Rate
Switches
Baud Rate
3
4
5
9600
1
0
0
9600
1
1200
1
0
1
14400
1
0
2400
1
1
0
19200
1
1
4800
1
1
1
38400
Data Bit Settings
Switch 6
Data Word Size
0
7 Bit Communication
1
8 Bit Communication
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Introduction to the Variable Wavelength Dector
Table 57
Parity Settings
Switches
Parity
7
8
0
0
No Parity
1
0
Odd Parity
1
1
Even Parity
One start bit and one stop bit are always used (not selectable).
Per default, the module will turn into 19200 baud, 8 data bit with no parity.
Forced Cold Start Settings
Switches 1 and 2 do not force storage of this set of parameters in non-volatile
memory. Returning switches 1 and 2 to other positions (other than being both
up) will allow for normal operation.
CAU TI O N
Forced cold start erases all methods and data stored in the non-volatile memory.
Exceptions are diagnose and repair log books which will not be erased.
If you use the following switch settings and power the instrument up again, a
forced cold start has been completed.
Table 58
Forced Cold Start Settings
Mode Select
1
2
3
4
5
6
7
8
TEST/BOOT
1
1
0
0
0
0
0
1
To return to normal operation, set switches back to your GPIB or RS 232
configuration settings.
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Introduction to the Variable Wavelength Dector
Stay-Resident Settings
Firmware update procedures may require this mode in case of firmware
loading errors.
Switches 1 and 2 do not force storage of this set of parameters in non-volatile
memory. Returning switches 1 and 2 to other positions (other than being both
up) will allow for normal operation.
If you use the following switch settings and power the instrument up again,
the instrument firmware stays in the resident part, that is, it is not operable as
a detector. It only uses basic functions of the operating system for example, for
communication.
Table 59
Stay Resident Settings
Mode Select
1
2
3
4
5
6
7
8
TEST/BOOT
1
1
0
0
1
0
0
0
To return to normal operation, set switches back to your GPIB or RS-232C
configuration settings.
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The Main Power Supply Assembly
The main power supply comprises a closed assembly (no on-site repair
possibility).
The power supply provides all DC voltages used in the module except for the
voltages supplied by the lamp power supply to the deuterium and tungsten
lamps in the detectors. The line voltage can vary in a range from 100–120 or
220–240 volts AC ± 10% and needs no manual setting.
M P S
+ 3 6 V
s u p p ly
lin e
v o lta g e
+ 2 4 V
s u p p ly
p rim a ry
c irc u its
se n se
+ 5 V
s w itc h in g
re g u la to r
lin e a r v o lta g e
re g u la to rs
Figure 53
WA RN ING
+ 3 6 V (n o t u s e d )
+ 2 4 V / 4 .5 A
+ 5 V / 3 A
+ 1 5 V / .3 A
-1 5 V / .3 A
Main Power Supply (MPS) Blockdiagram
To disconnect the instrument from line, unplug the power cord. The power supply
still uses some power, even if the power switch on the front panel is turned off.
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Introduction to the Variable Wavelength Dector
No accessible hardware fuse is needed because the main power supply is safe
against any short circuits or overload conditions on the output lines. When
overload conditions occur, the power supply turns off all output voltages.
Turning the line power off and on again resets the power supply to normal
operation if the cause of the overload condition has been removed.
An over-temperature sensor in the main power supply is used to turn off
output voltages if the temperature exceeds the acceptable limit (for example, if
the cooling fan of the instrument fails). To reset the main power supply to
normal operating conditions, turn the instrument off, wait until it is
approximately at ambient temperature and turn the instrument on again.
The following table gives the specifications of the main power supply.
Table 60
214
Main Power Supply Specifications
Maximum power
130 W
Continuous output
Line input
100–120 or 220–240 volts AC
± 10%, line frequency of 50/60 Hz
Wide ranging
Output 1
+ 24 V / 4.5 A (maximum)
Output 2
+ 36 V / 2.5 A (maximum)
Total power consumption of + 24 V
and + 36 V must not exceed 107 W.
Output 3
+5V/3A
Output 4
+ 15 V / 0.3 A
Output 5
- 15 V / 0.3 A
1100 Series Variable Wavelength Detector Reference Manual
Agilent 1100 Series Variable Wavelength Detector
Reference Manual
7
Control Module Screens for the Agilent
1100 Variable Wavelength Detector
Major keys on the Agilent 1100 Control Module 216
Screens available from the Analysis screen 217
Screens available from the System screen 227
Screens available from the Records screen 229
Diagnostics and Tests 235
Agilent Technologies
215
7
Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Major keys on the Agilent 1100 Control Module
Table 61
216
ESC
Return to previous screen and scroll through top layer views (Analysis, Settings)
m
Open context sensitive menus
i
Information/help
Enter
Store changed parameters or execute the choice from a pull-down menu
On/Off
Switch on lamp(s)
Start
Start a run
Plot
View the chromatogram
Views
Change between view of analysis - status - system screens
NO TE
The screens shown on the next pages are based on the following firmware revisions:
Control Module firmware revision B.01.01 (G1323B).
LC Module firmware revision 3.8x
NO TE
In case the control module’s display seems to be frozen (hang-up due to a communication
problem on the CAN bus, unplug the control module from the LC module and reconnect.
1100 Series Variable Wavelength Detector Reference Manual
7
Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Screens available from the Analysis screen
The Analysis screen
This is the wake-up screen, if the Agilent 1100 variable wavelength detector is
the only configured Agilent 1100 module. It is used to enter the most common
VWD method parameters.
The m-key allows access to the context sensitive menu. Setup view leads you
to add sections for additional Agilent 1100 modules. Restart re-boots the
control module.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Lamp ON/OFF
Use the F8 key (On/Off) to turn on the lamp(s). If more than one module is
available, select the VWD from the pop-up menu.
Setup View
In the Setup view, e.g. another module can be added to the view.
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7
Here, e.g. the diode array detector parameters are shown on the display as
well. The number of parameters on the display are restricted as additional
modules are added. Maximum 4 modules are shown automatically. If more
modules are in the system, you have to chose in Setup view.
With the Settings key you open a pull-down menu where you can select the
VWD modules.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Settings
Within the Settings you can change the VWD parameters and with a different
set of parameters available through the F1-5 keys. F7 key resets the VWD to
default values. F8 opens a window to turn on the lamp.
Use the m-key for the context sensitive menu. The Status command pulls up a
monitor screen displaying signals and spectra as programmed. Reset will load
the VWD default parameters. Balance brings the baseline back to set offset.
Use F1-key (More). You can enter special VWD setpoints.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Settings - Timetable
With the F2 key (Timetable) you can list the timetable for the VWD. Press F7
key (Insert) to add entries or F6 key (Delete) to remove entries.
Press the F7 key (Insert) to add a timetable events. Use the F6 key (Done) to
view the entered lines of the timetable.
Use the m-key for the context sensitive menu. It gives you additional tools for
the timetable.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Settings - Analog
With the F3 key (Analog) you can change the settings for the analog outputs.
Settings - Spectrum Range
With the F4 key (Spectrum) you can change the settings for the spectrum
acquisition, see “Sample, Blank and Holmium Spectrum" on page 237.
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7
Settings - Run times
With the F5 key (Runtimes) you can change the stop time and the post-run
time.
Press F5 key (Views) and select Status.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Status
This is an example if an Agilent 1100 VWD is configured standalone.
Information on the actual wavelength setting, absorbance value, elapsed run
time, messages and the signal plot are shown. Press key F8 (Start) to start a
run, key F7 (Rescale) to maximize the signal.
Signal plot
Press F6 key (Plot) to enter the plot screen (available also from the Analysis
and System screen). Here you can observe the online signal(s). To add
additional online signals (maximum 3), press F6 key (Select). If more than one
signal is configured (see next), use the 1-2-3 number key to switch between the
signals.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Press F6 key (Select). Here you can add additional online signals (maximum
are 3), press F6 key (Select). Additional signals could be also pressure or
temperature signals from other modules. Use the Right/Left arrows to switch
between Available and Selected Signals. Use the F8 key (Move) to enter
available signals into the box for selected signals or vice versa.
Method screens
On the Analysis screen use the F3 key (Method) to view the parameters in a
method and F8 key (Save As) to save the method in the module(s). The
PC-Card key is only active when a PCMCIA card is inserted in the control
module.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Use F2 key (PC-Card) to save a method on a PCMCIA card. Use the Right/Left
arrows to switch between PC-Card and Instrument window. Use the UP/Down
arrows to select the method. Use the F7/F8 keys (Copy) to enter available
signals into the box for selected signals or vice versa.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Screens available from the System screen
System screen
Use the Esc key to receive Views on the F5 key. Choose System from the
pull-down menu. This screen shows the last activities in the system.
System - Control
Use the F1 key (Control) to select the VW-Detector. Here you receive
information about the not-ready conditions if needed. F2 key (Reset) does a
re-initialization of the VWD.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
System - Configuration
On the System screen use the F2 key (Configure) to select the VWD. Here you
define further special setpoints for the VWD operation. Lamp type can be
either G1314-60100 (VWD) as the default lamp or 2140-0590 (DAD).
Use the F1 key (Interfaces) to access the interface settings (if required).
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7
Screens available from the Records screen
Records screen
Use the Esc key to receive Views on the F5 key. Choose System from the
pull-down menu. Use the F4 key (Records) to select the VWD. Errors are
reported either into the System Log (F2) or Error Log (F3).
System / Error Log
Use the F2 key (System Log) or F3 key (Error Log) to look for errors.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Info Log
Use the m-key to receive a pop-up menu, Select Info Log. A list of the last
events are listed. For troubleshooting reasons they can be printed or saved to a
file on the PCMCIA card (using the m-key for the context sensitive menu).
EMF (Early Maintenance Feedback)
Use the F1 key (EMF) to set EMF parameters. Choose menu item 1 (Setup
limits) to select lamp-on level for the UV lamp at which you want to receive a
warning.
230
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
If a set limit has been exceeded, a message box will pop up. This will not stop a
sequence or run (information only to plan maintenance activities). If you
press Reset, the limits will be removed. Ignore will continue to keep the EMF
flag set.
Firmware Update
Use the Esc key to receive Views on the F5 key. Choose System from the
pull-down menu. Use the F3 key (Records) to select the VWD. Use the F5 key
(FW Update) to enter the Update section. If you want to update the resident
firmware (together with specific main firmware revisions), select the a file
from the PCMCIA card (RESnnnn.DLB) and press execute. If you want to
update the main firmware, press F7 key (Transfer) to turn the module into
the resident mode (LED on module should blink yellow).
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Use the Esc key to receive Views on the F5 key. Choose System from the
pull-down menu. Use the F3 key (Records) to select the Generic module. In
this screen the resident firmware revision is shown.
Use the F5 key (FW Update) to enter the Update section. Select the a file from
the PCMCIA card (1314nnnn.DLB) and press execute. When the update has
finished, press F7 key (Transfer) to return the module into the normal mode
(LED on module should stay yellow).
If you have not saved your methods, please do it before continuing. Otherwise
they will be overwritten during the update process.
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7
Changing the serial number
In case the serial number of the module has to be added, use the m-key to open
the menu Enter Serial#. The serial number becomes active after restart of the
module.
Maintenance activities
On the Records screen use the F4 key (Maint log) to view and edit the
maintenance logbook.
1100 Series Variable Wavelength Detector Reference Manual
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7
Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Use the F7 key (Add) to add new maintenance activities. If an activity is not
listed, you can type the activity into the line “Add” using the control modules
key pad.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Diagnostics and Tests
Tests screen
Use the Esc key to receive Views on the F5 key. Choose System from the
pull-down menu. Use the F3 key (Tests) to select the VWD. Several tests are
available to test the Agilent 1100 VWD. Additional test are listed in the
function box. Refer to “Test Functions" on page 62 for more information on the
tests.
This screen shows in addition to functions the actual readings of the sample
and reference diodes that are useful to diagnose the flow cell condition.
Intensity Test
Use the F5 key (Intensity) to get the instrument profile (lamp on). Use the
Left/Right arrow to move the curser within the spectrum to find the
wavelength of interest.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Wavelength Calibration
Use the F1 key (Calibrate) and then F8 key (Execute) to start the VWD
wavelength calibration (with water in the flow cell). If a deviation is found,
press Adjust.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Sample, Blank and Holmium Spectrum
Use the F2 key (Spectrum) to take a spectrum (sample, blank or holmium).
Use the Left/Right arrow to move the curser within the spectrum to find the
wavelength of interest. To change the range refer to “Settings - Spectrum
Range" on page 222.
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Control Module Screens for the Agilent 1100 Variable Wavelength Detector
Built-in Test Chromatogram (Simulation)
To start the built-in test chromatogram, use the F8 key (Execute) to enable the
simulation. Then press the m-key to open the pop-up menu. Select Start to
start a run (all modules in the Agilent 1100 system must be in ready state).
Use the F6 key (Plot) to view the signal. A simulated chromatogram (4 peaks)
will repeat until the run is stopped. The signal is also available on the analog
output. A change of the peakwidth will change the retention times.
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7
Service Functions
On the System screen use the F3 key (Tests) to select the VWD. Use the m-key
for the context sensitive menu and select Service. The service screen allows
certain checks for diagnosing the VWD described in “Service Dialog" on
page 78.
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240
Control Module Screens for the Agilent 1100 Variable Wavelength Detector
1100 Series Variable Wavelength Detector Reference Manual
Agilent 1100 Series Variable Wavelength Detector
Reference Manual
8
Specifications
Performance Specifications 242
Agilent Technologies
241
8
Specifications
Performance Specifications
Table 62
242
Performance Specifications Agilent 1100 Series Variable Wavelength Detector
Type
Specification
Comments
Detection type
Double-beam photometer
Light source
Deuterium lamp
Wavelength range
190–600 nm
Short term noise
(ASTM)
± 0.75 × 10-5 AU at 254 nm
See NOTE on page 243.
Drift
3 × 10-4 AU/hr at 254 nm
See NOTE on page 243
Linearity
> 2 AU (5%) upper limit
See NOTE on page 243
Wavelength accuracy
± 1 nm
Self-calibration with deuterium lines,
verification with holmium oxide filter
Band width
6.5 nm typical
Flow cells
Standard: 14-µl volume, 10-mm
cell path length and 40 bar
(588 psi) pressure maximum
High pressure: 14-µl volume,
10-mm cell path length and
400 bar (5880 psi) pressure
maximum
Micro: 1-µl volume, 5-mm cell
path length and 40 bar (588 psi)
pressure maximum
Semimicro: 5-µl volume, 6-mm
cell path length and 40 bar
(588 psi) pressure maximum
Control and data
evaluation
Agilent ChemStation for LC
Analog outputs
Recorder/integrator: 100 mV or
1 V, output range 0.001 – 2 AU,
one output
Can be repaired on component level
1100 Series Variable Wavelength Detector Reference Manual
8
Specifications
Table 62
Performance Specifications Agilent 1100 Series Variable Wavelength Detector
(continued)
Type
Specification
Communications
Controller-area network (CAN),
GPIB, RS-232C, APG Remote:
ready, start, stop and
shut-down signals, LAN
optional
Comments
Safety and maintenance Extensive diagnostics, error
detection and display (through
control module and
Agilent ChemStation), leak
detection, safe leak handling,
leak output signal for shutdown
of pumping system. Low
voltages in major maintenance
areas.
NO TE
GLP features
Early maintenance feedback
(EMF) for continuous tracking
of instrument usage in terms of
lamp burn time with
user-settable limits and
feedback messages. Electronic
records of maintenance and
errors. Verification of
wavelength accuracy with
built-in holmium oxide filter.
Housing
All materials recyclable.
ASTM: “Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid
Chromatography”.
Reference conditions: cell path length 10 mm, response time 2 s, flow 1 ml/min LC-grade
methanol.
Linearity measured with caffeine at 265 nm.
1100 Series Variable Wavelength Detector Reference Manual
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8
244
Specifications
1100 Series Variable Wavelength Detector Reference Manual
Agilent 1100 Series Variable Wavelength Detector
Reference Manual
A
Safety Information
General Safety Information 246
Lithium Batteries Information 249
Radio Interference 250
Sound Emission 251
UV-Radiation 252
Solvent Information 253
Declaration of Conformity for HOX2 Filter 254
Agilent Technologies on Internet 255
Agilent Technologies
245
A
Safety Information
General Safety Information
The following general safety precautions must be observed during all phases of
operation, service, and repair of this instrument. Failure to comply with these
precautions or with specific warnings elsewhere in this manual violates safety
standards of design, manufacture, and intended use of the instrument. Agilent
Technologies assumes no liability for the customer’s failure to comply with
these requirements.
General
This is a Safety Class I instrument (provided with terminal for protective
earthing) and has been manufactured and tested according to international
safety standards.
Operation
Before applying power, comply with the installation section. Additionally the
following must be observed.
Do not remove instrument covers when operating. Before the instrument is
switched on, all protective earth terminals, extension cords,
auto-transformers, and devices connected to it must be connected to a
protective earth via a ground socket. Any interruption of the protective earth
grounding will cause a potential shock hazard that could result in serious
personal injury. Whenever it is likely that the protection has been impaired,
the instrument must be made inoperative and be secured against any intended
operation.
Make sure that only fuses with the required rated current and of the specified
type (normal blow, time delay, and so on) are used for replacement. The use of
repaired fuses and the short-circuiting of fuseholders must be avoided.
Some adjustments described in the manual, are made with power supplied to
the instrument, and protective covers removed. Energy available at many
points may, if contacted, result in personal injury.
Any adjustment, maintenance, and repair of the opened instrument under
voltage should be avoided as much as possible. When inevitable, this should be
carried out by a skilled person who is aware of the hazard involved. Do not
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Safety Information
attempt internal service or adjustment unless another person, capable of
rendering first aid and resuscitation, is present. Do not replace components
with power cable connected.
Do not operate the instrument in the presence of flammable gases or fumes.
Operation of any electrical instrument in such an environment constitutes a
definite safety hazard.
Do not install substitute parts or make any unauthorized modification to the
instrument.
Capacitors inside the instrument may still be charged, even though the
instrument has been disconnected from its source of supply. Dangerous
voltages, capable of causing serious personal injury, are present in this
instrument. Use extreme caution when handling, testing and adjusting.
Safety Symbols
Table 63 shows safety symbols used on the instrument and in the manuals.
Table 63
Symbol
!
Safety Symbols
Description
The apparatus is marked with this symbol when the user should refer to the instruction manual in order to
protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected ground terminal.
Eye damage may result from directly viewing the light produced by the deuterium lamp used in this
product. Always turn off the deuterium lamp before opening the metal lamp door on the side of the
instrument.
1100 Series Variable Wavelength Detector Reference Manual
247
A
Safety Information
WA RN ING
A warning alerts you to situations that could cause physical injury or damage to the
equipment. Do not proceed beyond a warning until you have fully understood and
met the indicated conditions.
CAU TI O N
A caution alerts you to situations that could cause a possible loss of data. Do not
proceed beyond a caution until you have fully understood and met the indicated
conditions.
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Safety Information
Lithium Batteries Information
WA RN ING
Danger of explosion if battery is incorrectly replaced. Replace only with the same or
equivalent type recommended by the equipment manufacturer. Lithium batteries
may not be disposed-off into the domestic waste.
Transportation of discharged Lithium batteries through carriers regulated by
IATA/ICAO, ADR, RID, IMDG is not allowed. Discharged Lithium batteries shall be
disposed off locally according to national waste disposal regulations for batteries.
WA RN ING
Lithiumbatteri - Eksplosionsfare ved fejlagtic handtering. Udskiftning ma kun ske
med batteri af samme fabrikat og type. Lever det brugte batteri tilbage til
leverandoren.
WA RN ING
Lithiumbatteri - Eksplosionsfare. Ved udskiftning benyttes kun batteri som anbefalt
av apparatfabrikanten. Brukt batteri returneres appararleverandoren.
NO TE
Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien
maar inleveren als KCA.
1100 Series Variable Wavelength Detector Reference Manual
249
A
Safety Information
Radio Interference
Never use cables other than the ones supplied by Agilent Technologies to
ensure proper functionality and compliance with safety or EMC regulations.
Test and Measurement
If test and measurement equipment is operated with equipment unscreened
cables and/or used for measurements on open set-ups, the user has to assure
that under operating conditions the radio interference limits are still met
within the premises.
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Safety Information
A
Sound Emission
Manufacturer’s Declaration
This statement is provided to comply with the requirements of the German
Sound Emission Directive of 18 January 1991.
This product has a sound pressure emission (at the operator position) < 70 dB.
• Sound Pressure Lp < 70 dB (A)
• At Operator Position
• Normal Operation
• According to ISO 7779:1988/EN 27779/1991 (Type Test)
1100 Series Variable Wavelength Detector Reference Manual
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Safety Information
UV-Radiation
Emissions of ultraviolet radiation (200-315 nm) from this product is limited
such that radiant exposure incident upon the unprotected skin or eye of
operator or service personnel is limited to the following TLVs (Threshold Limit
Values) according to the American Conference of Governmental Industrial
Hygienists:
Table 64
UV-Radiation Limits
Exposure/day
Effective Irradiance
8 hours
0.1 µW/cm2
10 minutes
5.0 µW/cm2
Typically the radiation values are much smaller than these limits:
Table 65
252
UV-Radiation Typical Values
Position
Effective Irradiance
Lamp installed, 50-cm distance
average 0.016 µW/cm2
Lamp installed, 50-cm distance
maximum 0.14 µW/cm2
1100 Series Variable Wavelength Detector Reference Manual
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Safety Information
Solvent Information
Observe the following recommendations on the use of solvents.
Flow Cell
Avoid the use of alkaline solutions (pH > 11) which can attack quartz and thus
impair the optical properties of the flow cell.
Solvents
Always filter solvents, small particles can permanently block the capillaries.
Avoid the use of the following steel-corrosive solvents:
• Solutions of alkali halides and their respective acids (for example, lithium
iodide, potassium chloride, and so on).
• High concentrations of inorganic acids like nitric acid, sulfuric acid
especially at higher temperatures (replace, if your chromatography method
allows, by phosphoric acid or phosphate buffer which are less corrosive
against stainless steel).
• Halogenated solvents or mixtures which form radicals and/or acids, for
example:
2CHCl3 + O2 → 2COCl2 + 2HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the stabilizing
alcohol.
• Chromatographic grade ethers, which can contain peroxides (for example,
THF, dioxane, di-isopropylether) such ethers should be filtered through dry
aluminium oxide which adsorbs the peroxides.
• Solutions of organic acids (acetic acid, formic acid, and so on) in organic
solvents. For example, a 1-% solution of acetic acid in methanol will attack
steel.
• Solutions containing strong complexing agents (for example, EDTA,
ethylene diamine tetra-acetic acid).
• Mixtures of carbon tetrachloride with 2-propanol or THF.
1100 Series Variable Wavelength Detector Reference Manual
253
A
Safety Information
Declaration of Conformity for HOX2 Filter
We herewith inform you that the
holmium oxide glass filter (type Hoya HY-1)
meets the following specification of absorbance maxima positions:
360.8 nm – 418.5 nm – 536.4 nm (spectral bandwidth: 1 nm).
Agilent Technologies guarantees the traceability of the specified absorbance
maxima to a National Institute of Standards & Technology (NIST) holmium
oxide solution standard with a lot-to-lot tolerance of ± 0.3 nm.
The wavelength calibration filter built into the Agilent Technologies UV-visible
detectors
Table 66
Wavelength Accuracy
Product number
79853C
HP 1050 Series
± 2 nm
G1314A
Agilent 1100 Series
± 1 nm
is made of this material and meets these specifications. It is, therefore,
suitable for wavelength calibration of these detectors within the specified
wavelength accuracy of the respective detector over its wavelength range.
November 1, 1995______________
DateQuality Manager
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Safety Information
Agilent Technologies on Internet
For the latest information on products and services visit our worldwide web
site on the Internet at:
http://www.agilent.com
Select “Products” - “Chemical Analysis”
It will provide also the latest firmware of the Agilent 1100 series modules for
download.
1100 Series Variable Wavelength Detector Reference Manual
255
A
256
Safety Information
1100 Series Variable Wavelength Detector Reference Manual
Index
A
address, 204
Agilent on internet, 255
analog signal output, 204
APG remote connector, 205
ASTM
environmental conditions, 13
ASTM reference and conditions, 243
control module
EMF, 230
firmware update, 231
serial number change of MWD, 233
test chromatogram, 238
tests, 235
control module, part number, 153
correction factors for flow cells, 30
cuvette holder, 97
B
D
band width 6.5 nm, 242
battery, 193
safety information, 249
BCD board description, 200
beam splitter assembly, 184
Beer-Lambert (law), 29
boards
interface boards (BCD), 200
photodiode boards (ADC), 184
dark current spectrum, 237
default address settings, 204, 209
delivery checklist, 15
detection type, 242
diagnostic signals
descriptions, 80
overview, 76, 79
diagram of controller, 194
dimensions, 14
drift, 242
C
cable
cabling at the rear, 18
connecting the analog, 20
connecting the APG remote, 20
connecting the CAN, 20
connecting the power, 20
overview and identification, 160
calibration
656 nm, 61
zero-order, 60
CAN interface, 204
connecting, 20
configuration switch
default settings, 208
description and factory setting, 208
connector locations, 20
E
electrical connections
descriptions of, 186
how to connect, 18
EMF
on control module, 230
EMF (early maintenance feedback), 189
entrance slit assembly, 182
error messages, 37
compensation sensor open, 45
compensation sensor short, 46
fan failed, 47
grating motor defective, 55
heater current missing, 52
holmium oxide test failed, 54
ignition without cover, 48
lamp current missing, 49
lamp ignition failed, 51
1100 Series Variable Wavelength Detector Reference Manual
lamp voltage missing, 50
leak, 42
leak sensor open, 43
leak sensor short, 44
remote timeout, 40
shutdown, 39
timeout, 38
wavelength check failed, 56
zero-order calibration failed, 53
ESD (electrostatic discharge) strap, 86
exchanging parts, see repairs
F
fan out, 205
features, 180, 242
GLP, 243
instrument layout, 188
safety and maintenace, 243
firmware
description, 197
flow diagram, 199
main system, 197
resident system, 197
updates, 198
updating, 135
firmware update with control module, 231
flow cell, 184
correction factors, 30
micro, 146
parts, 138
path length, 30
semimicro, 148
standard, 142, 144
types and data, 184, 242
front of detector, 19
fuses on BCD board, 200
257
Index
G
M
GPIB
default addresses, 209
interface, 204
grating assembly, 183
message
calibration lost, 56
compensation sensor open, 45
compensation sensor short, 46
fan failed, 47
grating motor defective, 55
heater current missing, 52
holmium oxide test failed, 54
ignition without cover, 48
lamp current missing, 49
lamp ignition failed, 51
lamp voltage missing, 50
leak, 42
leak sensor open, 43
leak sensor short, 44
remote timeout, 40
shutdown, 39
timeout, 38
wavelength check failed, 56
zero-order calibration failed, 53
messages, 37
mirror assemblies, 183
module status, 35
MPS (main power supply), 213
H
holmium oxide
declaration of conformity, 254
filter, 182
spectrum, 237
test, 65
I
information
on cuvette holder, 97
on lithium batteries, 249
installation
of flow connections, 22
of module, 19
interface
Agilent 1100 Series, 203
analog signal output, 204
BCD, 200
CAN, 204
GPIB, 204
remote, 205
RS-232C, 206
internet, 255
introduction
to optical system, 181
L
lamp, 182
turn-on routine, 63
type, 242
LAN cables, 178
LAN interface board, 202
line
power consumption, 14
voltage and frequency, 14
linearity, 242
258
N
noise, short term, 242
O
operating temperature, 14
optical unit
beam splitter, 184
components, 182
deuterium lamp, 182
entrance slit, 182
filter assembly, 182
flow cell, 184
grating, 183
layout of components, 182
mirrors, 183
optical path, 181
photodiode ADC boards, 184
photodiodes, 184
source lens, 182
optimization
detector performance, 28
overview
optical path, 181
optical system, 181
system overview, 181
P
parts identification
accessory kit, 159
analog cables, 162
auxillary cables, 174
BCD cables, 171
cable overview, 160
cables - LAN cables, 178
CAN cables, 175
control module, 153
cuvette holder, 152
external contacts cable, 176
flow cell, high pressure, 150
foams, 156
leak panels, 158
main assemblies, 138
micro flow cell, 146
optical unit and fan, 140
plastics, 154, 155
power and status, 157
remote cables, 165
semimicro flow cell, 148
sheet metal, 154
source lens and filter, 141
standard flow cell, 142, 144
performance
optimization, 28
performance specifications and
features, 242
photodiode
assemblies, 184
boards, 184
readings, 64
photometric accuracy, 30
physical specifications, 14
humidity, 14
line voltage and frequency, 14
operating temperature, 14
power consumption, 14
safety information, 14
weight and dimensions, 14
1100 Series Variable Wavelength Detector Reference Manual
Index
power consumption, 14
power supply, description of, 213
product specifications and features, 242
R
rear of detector, 20
recalibration of wavelength, 34, 236
remote
interface, 205
signal distribution, 205
repairs
assembling main cover, 133
cleaning the instrument, 85
correcting leaks, 100
exchanging fan, 111
exchanging flow cell, 91
exchanging holmium filter, 117
exchanging interface board, 134
exchanging lamps, 89
exchanging leak handling system, 101
exchanging leak sensor, 122
exchanging power supply, 124
exchanging processor board, 106
exchanging source lens, 114
exchanging status light pipe, 128
inside optical unit, 113
installing foams and top cover, 131
installing optical unit, 129
internal parts, 102
introduction, 84
overview, 87
overview of simple repairs, 88
removing optical unit, 120
removing top cover and foam, 103
replacing the firmware, 135
standard flow cell, 94
types simple/internal, 84
using the cuvette holder, 97
using the ESD strap, 86
warnings and cautions, 84
replacing parts, see repairs
RS-232C
cable kit to PC, 177
connection table, 206
interface, 206
settings, 210
S
safety information
on lithium batteries, 249
sample, holmium and dark current
spectrum, 237
serial number
entered on ChemStation, 110
entered on control module, 109, 233
serial number change with control
module, 233
service dialog, 78
service functions
screen, 239
setting the address, 209
site requirements, 12
bench space, 13
environment, 13
power considerations, 12
power cords, 12
source lens assembly, 182
specifications, 242
spectrum
range, 222
sample, blank, holmium, 237
stack configuration, 17
standards, 14
status indicators, 35
tests on control module, 235
troubleshooting
error messages, 34
status indicators, 34
U
unpacking, 15
using EMF, 189
V
VWM board
diagram of controller, 194
firmware description, 197
power interconnections, 196
W
wavelength
accuracy, 242
calibration, 58
range 190-600 nm, 242
recalibration, 34, 236
weight, 14
T
test chromatogram, 74
test chromatogram on control module, 238
test functions, 34
screen, 239
tests
656 nm calibration, 61
DAC (digital-to-analog), 71
dark current, 69
diagnostic signals, 76, 79
grating motor, 72
holmium oxide, 65
intensity of deuterium lamp, 67
photocurrent readings, 64
wavelength calibration, 58
zero-order calibration, 60
1100 Series Variable Wavelength Detector Reference Manual
259
Index
260
1100 Series Variable Wavelength Detector Reference Manual
s1
In This Book
This manual contains technical
reference information about the
Agilent 1100 Series variable
wavelength detector. The manual
describes the following:
• installing the detector,
• the detector optimization,
• diagnostics and troubleshooting,
• repairing the detector,
• parts and materials, and
• theory of operation and
introduction to the detector
• screens of local control module.
*G1314-90003*
*G1314-90003*
G1314-90003
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