221 and 222 XL Liquid Handlers User`s Guide

221 and 222 XL Liquid Handlers
User’s Guide
Errata 221/222 XL User’s Guide
page Appendix A-2
Model 221XL
Model 222XL
Part Number Qty Part Number Qty Description
...
27067213
...
1
27067213
1
Constricting needle, 316L,160.5 x 1.5 x 1.1 mm
page Appendix A-13
Model 221XL Model 222XL
Part Number Part Number Description
...
27067213
...
Ref : LT801483/A
27067213
Constricting needle 316L, 160.5 x 1.5 x 1.1 mm
221 and 222 XL Liquid Handlers
User’s Guide
LT801205/H - ©2003 Gilson SAS All rights reserved
April 2003
Contents
Table of Contents
0
Safety
1 Introduction
Unpacking ...................................................................................... 1-2
Warranty ......................................................................................... 1-3
Customer Service .......................................................................... 1-4
2 Description
Mechanical Assembly .................................................................. 2-2
Pumps.............................................................................................. 2-4
Electrical Assembly ...................................................................... 2-5
Electrical Control Socket ........................................................ 2-6
Microswitch Settings .............................................................. 2-6
Rinsing Stations ............................................................................. 2-8
3 Installing a Liquid Handler
Power Connection/Fuses ............................................................ 3-3
Voltage Selection ..................................................................... 3-3
Fuses .......................................................................................... 3-3
Connecting the Power Cord .................................................. 3-3
Installing the Vertical Arm .......................................................... 3-4
Needle/Liquid Level Detector ................................................... 3-5
Principle of the Liquid Level Detector ................................ 3-5
Sensitivity Limits ..................................................................... 3-5
Installation ................................................................................ 3-7
Gilson Sampler Controller Keypad ........................................... 3-8
Pumps.............................................................................................. 3-9
402 Syringe Pump ................................................................... 3-9
401 Dilutor ................................................................................ 3-9
Minipuls 3 Peristaltic Pump .................................................. 3-9
4 Installing The Additional Accessories
Heightening Kit ............................................................................. 4-2
Metallic Front Tray Assembly ..................................................... 4-3
Installation ................................................................................ 4-3
Levelling the Tray Platform .................................................. 4-5
Vertical Arms ................................................................................. 4-6
Needles ........................................................................................... 4-7
Constricting Needles .............................................................. 4-7
Teflon Needles ......................................................................... 4-7
Double Needles ....................................................................... 4-7
Table of Contents-1
TransferPorts™.............................................................................. 4-8
Installation ................................................................................ 4-8
Injection Ports ................................................................................ 4-9
Installation ................................................................................ 4-9
Thermostated Racks ................................................................... 4-10
Installation .............................................................................. 4-10
Perforated Covers for Thermostated Racks ..................... 4-12
Universal Rack ............................................................................. 4-13
Free Racks ..................................................................................... 4-14
Solvent Racks ............................................................................... 4-15
Needle Wiper ............................................................................... 4-16
Low Pressure Valve .................................................................... 4-17
5 Maintenance
Cleaning .......................................................................................... 5-2
Lubrication ..................................................................................... 5-3
Transportation ............................................................................... 5-4
Position Alignment Check ........................................................... 5-5
Appendices
A - Parts and Accessories Lists .............................. Appendix A-1
Standard Equipment.......................................... Appendix A-2
Accessories .......................................................... Appendix A-3
Gilson Sample Racks ......................................... Appendix A-4
FEP Tubing.......................................................... Appendix A-11
Parts Lists for 221 and 222 XL Generics ....... Appendix A-12
B - GSIOC ................................................................... Appendix B-1
Immediate Commands ....................................... Appendix B-2
Buffered Commands ........................................... Appendix B-5
C - Technical Data ..................................................... Appendix C-1
Hydraulics ............................................................ Appendix C-2
Mechanics ............................................................. Appendix C-3
Electrical ................................................................Appendix C-4
Control ................................................................... Appendix C-5
Programs ............................................................... Appendix C-6
Environmental Conditions ................................ Appendix C-7
D - Reference Information ...................................... Appendix D-1
Chemical Inertness of Plastic Used ................. Appendix D-2
Rack Compatibility Table ................................. Appendix D-3
Table of Contents-2
0
Safety
Safety
Read this section carefully before installing and operating the instrument.
For safe and correct use of the instrument, it is essential that both operating and service
personnel follow generally accepted safety procedures as well as the safety instructions
given in this document, the 221 and 222 XL Liquid Handlers User’s Guide.
The instruments described in this document are liquid handlers and should be used for
automatic liquid transfer operations only. They should only be used in the laboratory or
similar indoor environment for analytical purposes, by qualified personnel. If an
instrument is used in a manner not specified by Gilson, the protection provided by the
instrument may be impaired.
When the vertical arm and needle have been installed, it is necessary to check that nothing
hinders the movement of the instrument parts (i.e. needle and arms). Nothing should be
placed in or around the field of movement of either the horizontal or vertical arms.
Due to the fact that the needle installed on the vertical arm may contain a dangerous
substance, you are strongly advised against interfering in the work area of the
instrument until the instrument has come to a complete stop.
Voltages present inside the instrument are potentially dangerous. If there is a problem
with the instrument, the power cable should be removed until qualified service personnel
have repaired it. This is to prevent anyone from inadvertently using the instrument,
thus causing possible harm to themselves, or damage to the instrument itself.
The leakage current of this instrument is within the limits allowed by international
safety standards for laboratory equipment. An efficient ground connection is imperative
for the physical protection of the user.
Power supply cord reference 7080316106 is for use in France and Germany. Power
supply cord reference 7080316105 is for use in USA and Canada. For other countries
contact your local Gilson distributor. You must only use the type of fuse described in
this document: 0.5 Amp type “T” slow blow.
Safety-1
Safety
Adequate protection including clothing and
ventilation must be provided if dangerous liquids are
used in the analytical work. In case of incidental
spillage, carefully wipe with a dry cloth, taking into
account the nature of the spilled liquid and the
necessary safety precautions.
Cleaning, dismantling, maintenance, adjustment
and repair should only be performed by personnel
trained in such work, and who are aware of the
possible dangers involved. These instruments must
not be sterilized, using an autoclave, or any other
mechanical device. When you need to clean an
instrument, use one of the three following methods:
1 - a clean dry cloth,
2 - a cloth dampened with water,
3 - a cloth dampened with soapy water.
If a cloth dampened with soapy water is used to clean
the instrument, only domestic soap may be used. No
other form of detergent or chemical may be used.
Symbol Explanation
~
Alternating current
PROTECTIVE CONDUCTOR
TERMINAL
I
On (Supply switch)
O
Off (Supply switch)
Caution, risk of electric shock
!
Safety-2
Caution (refer to User’s Guide)
These electronic and hazard symbols appear on the
221/222:
Introduction
1
Unpacking ............................................................................ 2
Warranty ............................................................................... 3
Customer Service ................................................................ 4
This manual is intended for users of model 221 and 222 liquid handlers. Only procedures
described in this manual should be carried out by the user. 221 and 222 liquid handlers are
designed to work in a GSIOC environment. GSIOC (Gilson Serial Input Output Channel) is
a communications channel which links various Gilson modules together in a system.
Liquid handlers are slave XYZ robots which can be controlled either by computer or the
Gilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers
help automate liquid handling operations and sample preparation. They can be
configured as stand alone preparation devices, easy to use sample transfer instruments
or as systems for complete automation of sample preparation and transfer.
1-1
1
Unpacking
Introduction
Unpacking
Liquid handlers are delivered in a single box
containing both the instrument and its standard
parts (e.g. needles, tubing, etc...). The original box
and packing assembly should be kept in case the
equipment has to be returned to the factory and
especially while the instrument is under warranty.
All parts contained in the box must be checked off
by the user against the packing list supplied. This
should be performed as soon as possible even if the
instrument is not to be put to immediate use. The
supplier should be notified immediately of any
inconsistencies between the box contents and the
packing list and/or of damage to parts.
When unpacking your instrument:
• Remove and open the upper compartment
containing the standard parts (cables, needles, etc.),
• Slide the unit and packing assembly out of the box,
• Remove the locking screw,
• Remove packing assembly.
Horizontal arm
Arm-locking screw
Plastic plug
1-2
When delivered, the horizontal arm of the
liquid handler is fixed in its transportation
position by means of a screw. This screw
must be removed before the unit is installed.
It is located behind a plastic plug situated
on the right-hand side-panel (refer to the
opposite figure). The screw should be
replaced whenever the liquid handler is
transported, as failure to do so could result
in severe mechanical damage. If this screw
is lost, a replacement screw has been
provided and is situated at the top lefthand corner of the rear of the unit.
Introduction
If the instrument does not appear to function
correctly, first verify the electrical connections are
correct and that the instrument is switched ON.
Warranty
Warranty
1
Contact your Gilson distributor for technical advice
or an eventual service visit. Any service required
will be given within the warranty conditions
assured by your Gilson distributor.
1-3
1
Customer Service
Introduction
Customer Service
Gilson and its worldwide network of authorized
representatives provide you with four types of
assistance: sales, technical, applications and service.
The service personnel of Gilson's representatives are
able to serve you more efficiently if you provide the
following information:
1) The serial number and model number of the
equipment involved.
2) Computer model (if used), available memory,
microprocessor and operating software version(s)
in operation.
3) The installation procedure you used.
4) A concise list of the symptoms.
5) A list of operating procedures and conditions you
were using when the problem arose.
6) A list of other devices connected to the system
and a system diagram showing the connections.
7) A list of other electrical connections in the room.
1-4
Description
2
This chapter describes the different mechanical, electrical and hydraulic components of
the liquid handlers.
2-1
2
Mechanical Assembly
Description
Mechanical Assembly
Figures show the 221 and 222 in their standard
configuration.
To see which racks are compatible with the different
possible configurations, refer to the table on the last
page of this User's Guide.
Vertical arm
Needle holder
Liquid level
detector cable
Horizontal arm
Carriage casting
Needle
guide
Rinsing station
Support bar
Tray holding bar
2-2
2
Description
Mechanical Assembly
Rinsing station
Support bar
Tray rail
Hole containing spare arm-securing screw
Power socket
6-pin FCC type RS232 socket
for sampler controller keypad
Input/output
sockets
Microswitches
GSIOC
connector
25-pin D-type
RS232 socket
2-3
2
Pumps
Description
401 Dilutor
Pumps
The 221 and 222 units can be used with a 402
Syringe Pump, 401 Dilutor or Minipuls 3 peristaltic
pump connected to the needle.
All three pumps are shown in the following
diagrams.
Single Syringe and
Valve Configuration
The pump outlet should be connected to the needle
via the spiral transfer tubing (described in the
installation section).
The Minipuls 3 cannot be used to aspirate from a
vial.
Dual Syringe and
Tee Junction Configuration
Minipuls 3
2-4
Description
The rear panel of the liquid handler includes:
1. One sector block containing the power socket,
fuse holder and ON/OFF switch.
2. One 6-pin FCC type socket to which is connected
the sampler controller keypad via the RS232
coiled cable.
Electrical Assembly
Electrical Assembly
2
3. Four electrical control sockets- four inputs, seven
outputs (including three relay type) and one
analog input, one 12 V DC output and four
ground connections.
4. Eight microswitches which serve to identify the
machine and to activate certain functions such as
changing the baud rate, local clock, etc.
5. One GSIOC connector through which the 402
Syringe Pump, 401 Dilutor or Minipuls 3
peristaltic pump is connected.
6. One 25-Pin D-type RS232 socket for computer
connection.
Never connect a liquid handler to both a sampler
controller keypad and a computer simultaneously.
2-5
2
Electrical Assembly
Description
Electrical Control Socket
Located at the rear of the unit are four electrical
control sockets which consist of four contact inputs
and seven outputs (including three relay type outputs).
They also consist of one analog input which can be
configured to serve as a fifth contact input (refer to
“Microswitch Settings”).
The signal voltage of the analog input is + or - 100mV.
Use any of the cables supplied in the cable kit for
electrical connections (ref. 709910406) to coordinate
any peripheral instruments with the liquid handler.
Four barrier strip connectors are provided for this
purpose.
no nc c no nc c
1
no nc c
2
outputs
3
+
ground
4
5
6 7
ground
analog input/
5th contact input
1
2 3 4
ground
+12V ground
open collector
outputs
contact inputs
For liquid handlers, the analog input is normally
intended for connection to a detector but may be
used for other specific user applications.
Microswitch Settings
The eight micro-switches on the rear panel of the
liquid handler serve two purposes. They are used to
address the liquid handler and to activate certain
functions such as the baud rate, local clock, etc...
Microswitch Function
no
2-6
Up
Down
7
Clock
Local clock is selected only if
RS-232 socket is being used
Local clock is always enabled
6
Baud speed
Baud speed = 19200 bps
Baud speed = 9600 bps
5
Analog Input
Input is normal
Input acts as 5th contact
4
Unit ID number (in binary),
most significant bit
16
0
3
Unit ID
8
0
2
Unit ID
4
0
1
Unit ID
2
0
0
Unit ID number (in binary),
least significant bit
1
0
2
Description
The unit is factory set at 10. The diagram below
represents microswitches which have been set at a
GSIOC address of 10.
7
6
5
4
3
2
1
0
up
down
Electrical Assembly
Each switch is off when up and on when down.
Default setting
2-7
2
Rinsing Stations
Description
Rinsing well for level sensing
Drain
Rinsing
station
plug
Rinsing well
* There are three positions for
mounting the rinsing stations on
the support bar: A, left side, B, the
right of the center, and C on the
right end of the support bar.
Positions A and C are compatible with older versions of 222.
However, in this configuration,
the right station is close to the
right valve.
Position B offers better access to
the right injection valve and is
recommended as the standard
installation for the right rinsing
station on new samplers.
Rinsing Stations
Liquid handlers are equipped with rinsing stations
situated at the extremities of the support bar*. For
the 221 model there is only one rinsing station
located on the left hand-side of the support bar. As
the 222 model is larger, 2 rinsing stations are
provided, one at either end of the support bar. The
reason for this is to speed up the rinsing process.
When the needle is left of center of the support bar
during operation the needle is rinsed in the rinsing
station to the left of the bar and in the rinsing station
to the right of the bar if right of center. Although this
configuration is standard for a number of Gilson
applications, the user can, if desired, program the
needle to be rinsed in one rinsing station only.
Rinsing stations serve to clean the needle both
externally and internally.
The rinsing process is performed as follows:
1) A predetermined volume of rinsing solvent is
flushed through the needle, removing bubbles
from the FEP tubing and flushing any insoluble
matter from the needle.
This solvent is dispensed in the rinsing station
drain.
2) The needle is then plunged into the rinsing well
containing the solvent, which serves to rinse the
needle externally. Another volume of rinsing
solvent is then dispensed in the rinsing well.
Excess solvent in the rinsing well overflows into
the drain leading to a waste receptacle.
When the liquid level detector is activated the
smaller rinsing well is used. The user has the
possibility of removing the larger rinsing well and
replacing it with a special plug if the liquid level
detector is to be used for long periods. This prevents
the larger rinsing well from filling up with sediment.
Whenever the standard support bar is replaced, the
user must remember to remove the rinsing station
from the standard support bar and install it on the
replacement bar.
2-8
Installing a Liquid Handler
3
The liquid handler is delivered, factory-fitted with support bar, rinsing station (1 for the
221 model and 2 for the 222 model) and tray holding bar (refer to the figures on the next
page).
For safety purposes, the vertical arm is not installed but is supplied in the parts
compartment of the packing. The vertical arm must be installed by the user (refer to the
procedure described in chapter “Installing the Vertical Arm”).
3-1
3
Installing a Liquid Handler
221 XL
221 front panel
Rinsing station
Support bar
Tray
Tray holding bar
222 XL
222 front panel
Support bar
Rinsing
station
Tray
Tray rail
3-2
Installing a Liquid Handler
Voltage Selection
The sector block situated on the rear panel of the
unit contains the ON/OFF switch, power socket and
fuse holder/voltage selector.
Both the 221 and 222 models are pre-set to operate
on 240V. In order to change the voltage configuration,
check that the ON/OFF switch is in the OFF
position and the power cord is not connected to the
machine. Then, using a small screw-driver or
similar, pry the fuse holder open and remove it from
the power receptacle.
Power Connection/Fuses
Power Connection/Fuses
3
The voltages, either 110/120V or 220/240V indicated
at the rear of the fuse holder can be changed by
simply turning over the fuse holder, such that the
arrow corresponding to the required voltage, points
to the alignment mark on the power receptacle body.
It is important to check that the voltage selected
corresponds to that of the power supply used.
Fuses
The instrument requires two fuses to be installed.
The type of fuses required are 0.5 Amp type “T”
slow blow.
For safety reasons, liquid handlers are delivered
without fuses installed. Fuses must be installed by
the user upon delivery.
Connecting the power cord
Make sure the ON/OFF switch is in the OFF (down)
position. Connect the power cord to the power
socket at the rear of the unit and then plug in to the
power supply source. When the power cord has
been connected and the ON/OFF switch is put to
the ON position, a green LED on the front carriage
comes on.
3-3
3
Installing the Vertical Arm
a)
Before installing or changing the vertical arm, check
that the ON/OFF switch is in the OFF (down) position
and that the power cord has been disconnected from
the unit’s power socket. Check also that the horizontal
arm locking screw has been removed.
b)
a) Remove the black nylon screw from the tip of the
horizontal arm by turning it counterclockwise.
b) Carefully extract the hexagonal control rod by
pulling it outwards.
쒅
Installing the Vertical Arm
Installing a Liquid Handler
c) Move the slider to an intermediate position on
the horizontal arm.
This diagram shows the underside
of the horizontal arm and slider.
c)
쏟
d)
d1)Insert the vertical arm into the horizontal slider.
When viewed from above, the needle holder
must be on the right-hand side.
d2)Firmly secure the vertical arm by inserting the
two screws supplied, into the underside of the
slider and vertical arm.
d3)Connect the power cord and switch the ON/OFF
switch to its ON position. Wait while the motors
are reset to zero.
쒅
d4)When the arm has moved to its zero position, move
the needle holder upwards gently until it stops.
e1) Insert the hexagonal rod into the horizontal arm
so that it passes through the vertical arm’s
gearing and into the motor drive socket.
쒆
e)
e2) Move the needle holder downwards by about 1
mm from the upper limit, at the same time gently
push the rod until it clicks into position.
e3) Replace and tighten the black nylon screw.
Switch the ON/OFF switch to its OFF position.
쒅
f) If the size of the arm installed is not the same as
the one offered by Gilson in the standard
package, the Z-arm must be calibrated. Use the
Z-arm calibration software for XL samplers, or
call the local Gilson distributor for calibration.
The assembly process must be performed in the
order specified above so that the mechanical and
electrical components of the unit are coordinated.
The unit cannot operate correctly if the needle
holder is significantly more than 1 mm away from
the vertical arm’s upper limit.
3-4
Installing a Liquid Handler
The liquid level detector cable is an accessory. When
it is installed, the instrument no longer conforms to
EMC requirements.
A special feature of 221 and 222 Liquid Handlers is
the liquid level detector. It is used to detect when the
needle has reached the liquid surface which permits
the instrument to calculate the change in the liquid
level so that the tip of the needle always remains a
predetermined distance below the surface. It can
operate only when the liquid handler is used in
conjunction with a 402 Syringe Pump or a 401 Dilutor.
It does not function when the Minipuls 3 peristaltic
pump is used. The purpose of the liquid level
detector is to reduce the surface of the needle
coming into contact with the liquid thus reducing
contamination
Needle/Liquid Level Detector
Needle/Liquid Level Detector
3
Principle of the Liquid Level Detector
The needle descends into the tube until it reaches
the surface of the liquid. When this happens, a
signal is sent to the processor. From the moment the
processor receives this signal, it starts to calculate
the vertical speed required for the needle to follow
the liquid level as it changes. The vertical speed of
the needle is calculated as a function of the flow rate
and the cross sectional area of the tube entered.
Once the processor knows the rate at which the level
in the vial changes, it can adjust the speed of the
needle to remain a constant distance below the level
of the liquid.
Sensitivity Limits
The liquid level detector can only detect certain
liquids which have a dielectric constant greater than
18 (for a sensitivity value of 3). This means that
almost all water-soluble solvents (i.e. aqueous
solutions, common alcohols, acetone and
acetonitrile) are easily detected by the liquid level
detector. Among the solvents which cannot be
detected are hydrocarbons such as hexane,
cyclohexane and benzene and chlorinated solvents
such as chloroform and dichloromethane.
3-5
3
Needle/Liquid Level Detector
Installing a Liquid Handler
During normal operation, the recommended
immersion depth for the needle is 2 mm.
It is important to note that:
• The liquid level detector is not compatible with
septum piercing. The reason for this being that
with septum piercing, the needle sleeve must
always enter the tube to compensate for the
decrease of air pressure within the tube when the
liquid is aspirated.
• The liquid level detector does not function for
code 8 racks.
• The liquid level detector only functions with code
9 racks if the 402 Syringe Pump or 401 Dilutor is
installed with either a 0.5 ml or 1 ml syringe.
• The maximum recommended aspiration flow rates
in order to avoid immersion errors, are:
- 7 ml/min for small vials (in general, vials of
volume 4 ml or less),
- 10 ml/min for open tubes,
- 20 ml/min for 20 ml vials.
When the liquid level detector is activated, a precise
value must be entered for the inner diameter of the
vial. The vial diameter is measured in tenths of
millimeters. The Gilson program offers a default
value for each vial diameter at the programming
stage of the operation. As the rate at which the
needle descends or rises in the vial depends on the
inner diameter of the vial, it is important to enter a
value of within one tenth of a millimeter. Below are
some examples of possible errors generated by
entering the wrong internal tube diameter:
• For a code 21 rack, the default tube diameter is
11.3 mm. If there is an error of +/- 0.1 mm entered
for the tube diameter, then the resulting immersion
error will be +/- 1.5 mm. If there is an error of +/0.5 mm entered for the tube diameter then the
resulting immersion error is +/- 7 mm.
• For a code 9 rack, the default tube diameter is
6.1 mm. If there is an error of +/- 0.5 mm entered
for the tube diameter, then the resulting immersion
error will be +/- 4 mm.
The liquid level detector sensitivity can be adjusted
by the user at the programming stage of the operation.
For further information on sensitivity levels, etc.
refer to the software manual.
3-6
3
Installing a Liquid Handler
To install the needle/liquid level detector:
1) Slide the needle into the tubing fitting.
2) Select the FEP (polytetrafuoroethylene) transfer
tubing for the unit to dilutor connection which
corresponds to the volumes to be transferred.
3) Connect the selected tubing to the fitting.
4) Slide the needle assembly into the needle holder
on the vertical arm so that it passes through the
needle guide then connect the other end of the
tubing to the outlet of the 402 Syringe Pump, 401
Dilutor or the Minipuls 3 Peristaltic Pump.
5) Slide the ring contact of the liquid level detector
wire into the slot provided on the needle holder.
Check that the contact enters the slot fully so that
the tightening screw passes through the ring.
This is important as failure to do so could lead to
liquid level detection errors.
Needle
Ring
contact
Tubing
fitting
Needle/Liquid Level Detector
FEP tubing
Installation
Needle
holder
Liquid level detector wire
6) Connect the liquid level detector wire to the
coaxial socket situated just above the power-on
LED at the front of the carriage casting.
7) If larger diameter needles or probes such as
teflon coated probes are used, you may need to
mount specific needle holders and needle guides
instead of the standard ones.
Needle
guide
3-7
3
Gilson Sampler Controller Keypad
Installing a Liquid Handler
Gilson Sampler Controller Keypad
The Liquid Handlers are available with either
735 Sampler Controller Software or with the Keypad
Controller and the 720 software.
For installation of the 735 Sampler Controller
Software, refer to the relevant User’s Guide.
The Sampler Controller Keypad supplied in the
parts compartment of the packing assembly controls
the liquid handler and connected pumps.
The keypad is connected to the instrument via an
RS232 coiled cable. Characters are displayed in
white on a blue background.
The keypad consists of:
- an eight-line, 40 character visual display unit,
- five menu soft-touch keys just below the display
unit,
- a numerical keypad with an ENTER soft-touch key,
- four cursor direction soft-touch keys,
- a group of three function soft-touch keys , HELP,
ESC., CLEAR.
- a 3.5" diskette drive with protective cover.
Type of diskettes used are 3.5" high density diskettes.
For information on using the keypad refer to the
software manual.
3-8
Installing a Liquid Handler
Liquid handlers can operate in conjunction with a
402 Syringe Pump, 401 Dilutor or a Gilson Minipuls 3
Peristaltic Pump.
Pumps
Pumps
3
402 Syringe Pump
The 402 Syringe Pump is a slave pump and is
connected to either the 221 or 222 Liquid Handler
via a GSIOC (Gilson Serial Input/Output Channel)
connector located on the rear panel of the 402
Syringe Pump. Refer to the 402 User's Guide.
401 Dilutor
The 401 Dilutor is a slave pump and is connected to
either the 221 or 222 Liquid Handler via a GSIOC
(Gilson Serial Input/Output Channel) connector
located on the rear panel of the 401 Dilutor. Refer to
the 401 User’s Guide.
Minipuls 3 Peristaltic Pump
The Minipuls 3 Peristaltic Pump is connected to 221
and 222 Liquid Handlers via a GSIOC connector.
It is important to note that the liquid level detector
does not function with a Minipuls 3 Peristaltic Pump,
and that you cannot aspirate with the Minipuls 3.
Refer to the Minipuls 3 Peristaltic Pump User's Guide.
3-9
Installing a Liquid Handler
3-10
3
Installing the Additional Accessories
4
The reference and descriptions of the additional accessories are available in Appendix A,
“Accessories” .
4-1
4
Heightening Kit
Installing the Additional Accessories
Heightening kit
This kit is used to heighten the unit so that longer
tubes may be used. When this kit is used, a 183 mm
stroke arm must be installed. Using this kit means
that racks are used in the lower position. When the
heightening kit is installed, racks of codes 22, 33 and
34 should be entered as 92, 93 and 94 respectively.
It is available for Model 222 Liquid Handlers only. It
contains 4 heightening legs and two tray rail
support bars. The legs are fitted to the base of the
liquid handler. To install these legs simply place the
liquid handler on its side and locate the four holes
situated at each corner of the base of the unit. Once
located, simply screw-in the four heightening legs
and redress the unit. The two tray rail support bars
are fitted to the front panel of the liquid handler
using the four screws supplied. The tray rail is fitted
to the tray rail support bars via the hole situated
near the base of each tray rail support bar.
Support bar
Tray rail
Heightening legs
Tray rail support bar
4-2
4
Installing the Additional Accessories
Metallic Front Tray Assembly
Metallic Front Tray Assembly
The metallic front tray assembly is a heightening
and support kit which is available for 222 Liquid
Handlers only. It is used for certain applications (i.e.
pathological tissue staining, glue dropping, etc.)
where it is necessary to have a flat working surface.
The metallic front tray assembly consists of:
-
a tray platform,
two platform support arms,
two adjustable supports,
four heightening legs (65 mm).
Installation
The user may install the tray platform in either the
upper or lower position, depending on the application,
and on the length of the vertical arm installed on the
liquid handler (lower position for the 183 mm arm).
Four screws, with black plastic knurled heads, are
provided for the purpose of attaching the tray
platform to the platform support arms. The tray
platform is fixed either above the platform support
arms for the 'upper' position, or below the platform
support arms with the tray rail uppermost and
nearest to the 222 for the 'lower' position.
Support bar
Platform
support
arm
Tray rail
Tray platform
Heightening legs
Adjustable support
4-3
Metallic Front Tray Assembly
Installing the Additional Accessories
4
To install the legs simply place the liquid handler on
its side and locate the four holes situated at each
corner of the base of the unit. Once located, simply
screw-in the four heightening legs and redress the
unit. The two metallic platform support arms are
fitted to the front panel of the unit using the four
screws supplied.
When a 183 mm vertical arm is used the tray
platform must be in the lower position. This is to
avoid damage to the needle during operation.
When a 123 mm vertical arm is used, the tray
platform must be in the upper position.
Installing the Platform in the Upper Position
To install the tray platform simply:
1) Remove the two plastic headed screws from the
top of the support arm.
2) Place the platform on the arm, with the tray rail
uppermost and nearest the arm angle. Replace
the two screws by passing them through the two
corresponding holes in the platform. Secure them
manually.
3) Repeat the procedure for the other arm.
4) Install the platform and arm assembly onto the
front panel of the machine using the two screws
provided on each arm.
Installing the platform in the lower position
For some applications (notably, when using the 183 mm
stroke arm) it is necessary to install the platform in its
lower position, beneath the support arms as follows:
1) Remove the two plastic headed screws and the
adjustable support from the support arm.
2) Position the platform beneath the support arm
with the tray rail uppermost and nearest the
angle. (i.e. the tray rail towards the machine
when the assembly is installed).
3) Locate the two smaller holes in the base of the
support arm and position the arm such that these
holes correspond to the two smaller holes in the
platform.
4-4
Installing the Additional Accessories
5) Locate the third hole in the base of the platform
and insert the adjustable support.
6) Repeat the procedure for the other arm.
7) Install the platform and arm assembly onto the
front panel of the machine using the two screws
provided on the arm.
Levelling the tray platform
The tray platform must be horizontal for correct
needle operation. Two adjustable supports are
provided on the underside of each support arm for
this purpose.
Metallic Front Tray Assembly
4) Secure the platform to the arm from beneath
using the two plastic headed screws.
4
Adjustment is performed as follows:
1) Manually loosen the support’s locking collars
located at the top of each support, by turning
them clockwise.
2) Turn the foot of each support to level the tray
platform. Check, using a spirit level, that the
workbench, liquid handler and platform are
level. However, this adjustment is not so critical
as to require an absolutely level reference surface.
3) Manually tighten the support’s locking collars, by
turning them counterclockwise (when viewed
from above).
Installing the rack-tray
Place the rack-tray on top of the tray platform with
the groove on the underside of the rack-tray firmly
slotted over the tray rail.
4-5
Vertical Arms
Installing the Additional Accessories
4-6
4
Vertical Arms
The installation procedure for the long and short
arms and needle kits is as described in Chapter 3.
When the user has changed the size of the vertical
stroke arm, it is necessary to call the local Gilson
distributor for recalibration of the machine as the
machine will take the arm size as being 123 mm by
default.
Installing the Additional Accessories
Various needle types are available for use with both
221 and 222 Models. They are:
Needles
Needles
4
Constricting needles
When using TransferPorts™, beveled edge constricting
needles must be used.
These needles are specifically designed for dealing
with small volumes. They have a specially designed
tip which reduces the risk of cross contamination
between samples and increases measuring accuracy
when dealing with small volumes.
Refer to Chapter 3 for installation.
Teflon needles
Teflon needles are chemically inert needles. They are
used for applications where metal traces such as
stainless steel and titanium, from which needles are
normally made, should be avoided.
Refer to Chapter 3 for installation.
Double needles
Double needles consist of both an inner and an outer
tube which act as two separate needles. They can
either be used to mix two different samples in the
same vial or for sample agitation or evaporation.
Sample agitation and evaporation is performed by
sending a gas into the vial through either the inner
or outer tube and the sample through the remaining
tube. The gas creates bubbles which agitate the
sample.
Refer to the instructions leaflet supplied with the
double needle kit for installation.
4-7
TransferPorts™
Installing the Additional Accessories
TransferPort holding bar
TransferPorts™
Yellow cap
In some configurations, TransferPorts may be used
to aspirate large volumes of either a sample or a
solvent. The 221 has 8 possible positions and the 222
has 15 possible TransferPort positions.
Plastic
cap
Connector
Connector
tubing
1) Users are advised to place the
bottles containing the samples
which are to be aspirated
through the TransferPorts, at
the same level or higher, than
the rack tray.
2) When working with TransferPorts, the user must always use
a bevelled edge constricting
needle.
3) TransferPorts must be primed
using the program before
being used.
TransferPort is a trademark of
Gilson.
4-8
4
As TransferPorts are an additional accessory, it is
necessary to replace the standard support bar with
the specific TransferPort holding bar (ref. 2949192
for 221 and 2954706 for 222) which consists of a
series of numbered holes. To change the standard
support bar simply unscrew the knurled screws
securing it and replace it with the transfer port
holding bar. A bevelled edge constricting needle
must be installed by the user if TransferPorts are to
be used.
Installation
TransferPorts are installed as follows:
1) Place the TransferPort in the appropriate
mounting hole on the transfer port holding bar
and screw the TransferPort and tubing connector
together.
2) Fit a yellow cap to the top of the TransferPort.
3) Connect the other end of the male tubing to a
filter, and place it in a solvent or sample bottle.
4) Fix the plastic clip onto the TransferPort.
Installing the Additional Accessories
When a sample is prepared, it can be injected into a
peripheral instrument via an injection port which
can be installed on the support bar.
Injection Ports
Injection Ports
4
Installation
The injection port is installed in the hole supplied to
the right hand side of the standard support bar as
follows:
1) Separate the upper part of the injection port,
(with hexagonal head) from the main body by
unscrewing it.
2) Insert the upper part of the injection port into the
specific hole to the right of the support bar and
screw in the main body from beneath.
When the seal (2954674) is worn, simply extract it
using a needle or similar and replace it.
4-9
Thermostated Racks
Installing the Additional Accessories
4
Thermostated Racks
Thermostated racks consist of an aluminium rack
and a thermostating cuvette. There are two types of
thermostating cuvette: one regulated by a circulating
fluid, and the other regulated by Peltier effect cells,
electronically controlled by the Gilson 832 Temperature
Regulator.
They are used to regulate the sample temperature,
e.g. cooling for the preservation of biological samples
and moderate heating for chemical derivatization.
Possible temperatures range from 4 to 60°C for the
fluid regulated cuvette and 4 to 40°C for the peltier
effect thermostating cuvette.
• The fluid regulated cuvette is available under
reference 2704429.
• The Peltier effect thermostating cuvette is available
under reference 2759550 and must be used in
conjunction with the Gilson 832 Temperature
Regulator, reference 2759510.
Installation
When the aluminum thermostating
racks are used on the 222, some of
them can be placed different
positions. As a result, the
identification code used for the
rack changes so that the different
rack and vial coordinates can be
recognized by the software.
The rack code is the number by which the rack is
known to the software. This is extremely important
to remember at the programming stage of the
application. Refer to Appendix A for the correct code
number, reference number and corresponding
position of all racks. The unperforated rack must be
declared as a custom rack.
Rack code Actual code Position Equipment required
for software
on rack
4-10
30
(30)
Normal Metallic Holder (2704473) and thermostating cuvette.
31
(31)
Normal Metallic Holder (2704473) and thermostating cuvette.
32
(32)
Normal Metallic Holder (2704473) and thermostating cuvette.
33
(33)
Normal Metallic Holder (2704473) and thermostating cuvette.
34
(34)
Normal Metallic Holder (2704473) and thermostating cuvette.
93
(33)
Low*
Heightening kit for sampler and thermostating cuvette.
94
(34)
Low*
Heightening kit for sampler and thermostating cuvette.
Custom rack
no code
Normal Metallic Holder (2704473) and thermostating cuvette.
This rack (2704499) is unperforated (a free rack) and
must be drilled according to your requirements, then
declared in the PC software tray configuration.
4
Installing the Additional Accessories
1) Remove the standard support bar and install the
specific thermostated rack holding bar (reference
2704471M).
2) Install the rack in the thermostating cuvette.
3) Position the cuvette containing the rack, on the
rack holding bar.
* Racks in the normal position
are placed in the thermostating
cuvette and mounted on the
metallic rack holding bar.
Racks in the low position are
placed in the thermostating
cuvette and mounted on the
tray. The sampler is in the
raised position using the
heightening kit, reference
2749870.
4) Connect the hydraulic connections/connect the
control cable to the 832 if the thermostating
cuvette is the Peltier effect type.
Thermostated Racks
Installing a Thermostated Rack on the 221 XL
Installing a Thermostated Rack on the 222 XL
1) For the racks installed on the tray, install the
heightening kit on the sampler (reference 2749870).
Follow the instructions supplied with the kit.
2) For the racks in the normal position, replace the
standard support bar with the rack holding bar
supplied in the rack holder kit (reference 2704473).
Fix the rack holder to the tray rail following the
instruction supplied with the kit.
3) Loosen the two nylon screws on the front panel
of the rack holder.
4) Tilt the front panel forwards.
5) Place the rack inside the thermostating cuvette.
Rack holding bar supplied
in the rack holder kit
Thermostated rack holder
When installing a thermostated
rack on the 222 model, there are
two positions:
Tray rail (installed on instrument)
•'normal' for a rack installed on
the rack holding bar (kit
reference 2704473)
•'low' for a rack installed on the
tray the sampler must be fitted
with a heightening kit (kit
reference 2749870).
4-11
4
Thermostated Racks
Installing the Additional Accessories
6) Install the cuvette in one of the five possible
positions. Each position is marked by two
opposite pins which serve to locate and secure
the cuvette. One pin is situated on the holding
bar, the other at the rear of the front panel of the
rack holder. Position the notches on the
polypropylene cuvette over the locating pins.
7) Tighten the two black nylon screws on the front
panel of the rack holder.
8) Connect the hydraulic connections/connect the
control cable to the 832 if the thermostating
cuvette is the Peltier effect type.
Perforated Covers for Thermostated
Racks
When thermostated racks are used to cool samples
below the ambient temperature, condensation forms
on the surface of the rack and falls into the rack
wells. This water accumulates in the wells and
forces the vials in the rack to rise. If this happens,
needle movement may be obstructed and the needle
damaged. For this reason stainless steel perforated
covers have to be installed. They are clipped onto
the rack handles as shown. These covers are
available for rack codes 30A, 31A, 33A and 34A.
Below a list of the perforated covers available.
4-12
Reference
Description
27044307
30A rack cover, stainless steel, 60 holes
27044317
31A rack cover, stainless steel, 108 holes
27044337
33A rack cover, stainless steel, 14 holes
27044347
34A rack cover, stainless steel, 36 holes
Installing the Additional Accessories
Universal racks (code 22U) are designed to
receive 44 vessels of varying sizes. The sizes
of these vessels range from 10 mm to 18 mm
external diameter and from 100 to 180 mm in
length. Each of the reception cavities contains
4 positioning and retaining clips.
Universal Rack
Universal Rack
4
4-13
Free Racks
Installing the Additional Accessories
Free Racks
2704599
2704499
Free racks are blank racks with no vial positions
drilled. These racks can only be used with softwares
that offer free rack programming (custom rack
option), essentially PC based softwares (735 and
719).
Reference 2704599 is a free rack in polypropylene.
Reference 2704499 is a free thermostating rack in
aluminum.
Drill the vial positions according to your method
requirements, then declare the coordinates in the
software configuration.
4-14
4
4
Installing the Additional Accessories
Solvent Racks
Solvent Racks
Solvent racks code 60 (reference 2954651) and 61
(reference 2954715) hold up to four solvent bottles.
Gilson supplies two types of solvent bottles, 250 ml
polypropylene bottles and 180 ml glass bottles.
Select bottles which are comecure the bottles in
position and ensure that the needle penetrates each
bottle cap correctly.
When using rack code 61, a 183 mm vertical stroke
arm, corresponding needle and the heightening kit
must be installed.
Code 60
Code 61
4-15
Needle Wiper
Installing the Additional Accessories
4
Needle wiper
Needle wipers are used to reduce contamination
risks when using rack codes 20, 21, 28 or 29.
They wipe excess liquid from the exterior of the
needle as it is being withdrawn from the sample.
Needle wipers consist of three perforated plastic
plates between which are held two sheets of
absorbent paper. Refer to the opposite figures for the
needle wiper assembly.
4-16
4
Installing the Additional Accessories
Low Pressure Valve
Low Pressure Valve
The low pressure valve is an electronically operated
(12 VDC) 3-port, 2-position valve and is used for the
purpose of evaporating a solvent with an inert gas
or mixing liquids contained in a vial by permitting
the passage of air into the liquids (the MIX by air
method - refer to the software User’s Guide.
The valve has three connections: two inlets, a
normally closed (NC) inlet, and a normally open
(NO) inlet; and one common (COM) outlet. This is
the default position of an unpowered valve. The
valve receives its power from the connector on the
carriage casting.
Installation
1) Remove the screw situated on the upper left hand
corner of the front panel of the vertical arm.
2) Slide the valve attachment piece onto the front
panel of the vertical arm until the hole at the top
left hand corner of the attachment piece
corresponds to the hole left in the arm by the
removal of the screw.
3) Fix the valve to the vertical arm using the
attachment screw supplied.
4) Connect the 402 Syringe Pump, 401 Dilutor or
Minipuls 3 Peristaltic Pump to the NO (normally
open) inlet port of the valve.
5) Connect the FEP tubing, (already connected to
the needle assembly), to the valve outlet port.
6) Connect the air supply (or inert gas or alternative
fluid) to the valve via the NC (normally closed)
inlet port.
7) Once hydraulic and pneumatic connections have
been made, the valve can be connected to the
12 VDC output situated just below the power-on
LED at the front of the carriage casting.
The maximum allowable working
pressure of the valve is 0.138 MPa.
4-17
Installing the Additional Accessories
4-18
4
Maintenance
5
The ON/OFF switch must always be switched to OFF and the unit
disconnected from the mains whenever a maintenance operation is to be
performed.
Only maintenance operations described here should be carried out by the
user. Other maintenance operations not described here should be referred
to qualified service personnel.
5-1
5
Cleaning
Maintenance
Cleaning
Cleaning, dismantling, maintenance, adjustment
and repair should only be performed by personnel
trained in such work, and who are aware of the
possible dangers involved.
These instruments must not be sterilized, using an
autoclave, or any other mechanical device. When
you need to clean an instrument, use one of the
three following methods:
1) A clean dry cloth.
2) A cloth dampened with water.
3) A cloth dampened with soapy water.
If a cloth dampened with soapy water is used to clean
the instrument, only domestic soap may be used.
No other form of detergent or chemical may be used.
5-2
Maintenance
Model 221 and 222 Liquid Handlers should never be
lubricated. The user is strongly advised against
oiling or greasing any moving part such as guiding
rods, gears, belts etc.
Lubrication
Lubrication
5
5-3
5
Transportation
Maintenance
Transportation
If the liquid handler is to be transported, the user is
advised to remove the vertical arm and replace the
horizontal arm locking screw. Failure to do so could
result in misalignment or belt breakage.
When transporting over shorter distances (i.e.
within the laboratory etc...) the liquid handler may
be carried by its support bar.
Liquid handlers must, on no account, be picked up
or carried by either their vertical or horizontal arms
as belt breakage or misalignment could occur.
5-4
Maintenance
Liquid handlers leave the factory perfectly aligned
and do not normally require checking. As previously
mentioned, misalignment may be caused by
transporting the unit without having previously
secured the horizontal arm or by carrying the unit
by its arms. If misalignment should occur, it is
possible to check alignment by examining the
placement of the needle in the rack position. If there
is any doubt about alignment call service.
in the recess using a soft cloth or an air line.
Position Alignment Check
Position Alignment Check
5
5-5
Maintenance
5-6
5
Parts & Accessories Lists
Appendix
A
Parts lists for the 221 and 222 XL Liquid Handlers, consisting of Standard equipment,
Accessories, Racks and Spare parts.
Appendix A-1
Standard Equipment
Parts & Accessories Lists
Appendix
A
Standard Equipment
Model 221XL
Model 222XL
Part Number Qty Part Number Qty Description
270021
271021735
221XL Liquid Handler, 110-220 V
221XL Liquid Handler with 735 Software, 110-220 V
21067720
271052
271052735
222XL Liquid Handler, 110-220 V
222XL Liquid Handler with 735 Software, 110-220V
21067720
Gilson 720 sampler controller software package
(only with 270021 and 271052)
27072001
F1410153
49967202
49967392
27067211
27077213
4701177592
27062634
2707255
1
1
1
1
1
1
1
1
1
27072001
F1410153
49967202
49967392
27067211
27077213
4701177592
27062634
2707255
1
1
1
1
1
1
2
1
1
Needle tubing fitting
PVDF union connector for 1/4” - 28 TPI fittings
FEP tubing, 500 x 2.5 x 1.5 mm, 800 µl
FEP tubing, 1000 x 2.5 x 1.5 mm, 1600 µl
Bevelled edge needle, 316L, 160.5 x 1.5 x 1.1 mm
Constricting needle, 316L,160.5 x 1.5 x 1.1 mm
Rinsing station drain tubing, 1000 x 6.5 x 4 mm
Needle sleeve holder, polyacetal, 2.1 mm hole with screws
Rinsing station plug
54081252
1704731
270620
3
1
1
54081252
15074002
270620
3
1
1
Adhesive retainer for transfer tubing
Polypropylene tray
123 mm stroke vertical arm
270230
36078143
360831221
52903071
638306512
2749592
7080316105
7080316106
6730054006
1
1
1
1
4
1
1
1
5
270230
36078143
360831221
52903071
638306512
2749892
7080316105
7080316106
6730054006
1
1
1
1
4
1
1
1
5
Sampler controller keypad with RS 232 coiled cable
GSIOC cable for 22X to 401, 402 or Minipuls connection
RS232 cable for connecting sampler to PC
Self-adhesive label for GSIOC identification
Barrier strip connectors, 6-pin for electrical control socket
Liquid level detector wire (400 mm for 221, 480 mm for 222)
Power cord - 100-120 V, 50-60 Hz
Power cord - 220-240 V, 50-60 Hz
Fuses 0.5 Amp type “T” slow blow
LT801205
1
LT801205
1
221 and 222 Liquid Handlers User’s Guide
Appendix A-2
Parts & Accessories Lists
Appendix
A
Accessories
Accessories
Arms and needle related items
With 221 and 222 Liquid Handlers, there is the
possibility of replacing the standard 123 mm stroke
vertical arm with either a 56 mm or 183 mm stroke
arm.
The 56 mm stroke arm is used for racks in the upper
position containing vials with septa.
The 183 mm stroke arm is for use with longer tubes.
Below is a list of the various accessories which can
be used in conjunction with each of the three arms:
Model 221XL Model 222XL
Part Number Part Number Description
270610
27067271
270610
27067271
27077113
27077113
27067212
27067212
27067276
27067277
27367513
27067276
27067277
27367513
27167215
27167215
270630
27067361
27067373
27067374
27067362
27367423
2749870
For the 56 mm stroke arm
56 mm stroke vertical arm
Bevelled edge constricting needle, for use with TransferPorts,
316L, 93.5 x 1.5 x 1.1 mm
Constricting needle, 316L, 93.5 x 1.5 x 1.1 mm
For the 123 mm stroke arm
Side aperture needle for septum piercing and/or liquid mixing,
316L, 160.5 x 1.5 x 1.1 mm
Flat tipped needle for drop transfer, 316L, 165 x 0.7 x 0.4 mm
Needle sleeve for 27067276, 155 mm, flat tipped without “V”-cut
Teflon needle kit for applications where contact with metal is
undesirable (contains: Teflon needle, 157.5 x 0.9 i.d. ; Special needle
holder ; Needle guide)
Double needle kit (contains: Flat tipped needle, 181.5 x 1.5 x 1.1 mm ;
Triangular flat tipped needle, 169.5 x 2.5 x 1.1 mm ; PVDF connector ;
Special needle holder ; Needle guide)
For the 183 mm stroke arm
183 mm stroke vertical arm
Bevelled edge needle, 316L, 220.5 x 1.5 x 1.1 mm
Constricting needle, 316L, 220.5 x 1.5 x 1.1 mm
Bevelled edge constricting needle, for use with TransferPorts™,
316L, 220.5 x 1.5 x 1.1 mm
Side aperture needle, for septum piercing and/or liquid mixing
316L, 220.5 x 1.5 x 1.1 mm
Teflon needle for applications where contact with metal is
undesirable (contains: Teflon needle, 220.5 x 0.9 mm i.d. ; Special
needle holder ; Needle guide)
222 Heightening kit (contains: 4 heightening legs, 65 mm ; 2 tray rail
support bars)
Appendix A-3
Accessories
Parts & Accessories Lists
Appendix
A
Other Accessories
Model 221XL Model 222XL
Part Number Part Number Description
2749767
2949192
2954709
2749767
2954706
2954709
Injection port
TransferPort holding bar (221: 8 tubes, 222: 16 holes)
TransferPort accessory kit (contains: Polyamide transfer port ;
2800 µl, FEP tubing, 1000 x 3 x 2 mm, fitted with filter 316L, 40 µm ;
Sealing caps)
130742
2707404
2749872
Holding bar for racks series 0 to 10
Metallic front tray assembly (contains: Tray platform and arms
150478
150471
15047110
36077240
150478
150471
15047110
36077240
Needle wiper for code 20 and 28 racks
Needle wiper for code 21 and 29 racks
Sheets of wiping paper (pack of 500)
3 port, low pressure valve kit
49963242
49962392
49967202
49967392
49948392
2954583
2954671
29425066
2954672
49963242
49962392
49967202
49967392
49948392
2954583
2954671
29425066
2954672
120 µl FEP tubing (600 x 1.6 x 0.5 mm)
440 µl FEP tubing (100 x 1.6 x 0.8 mm)
800 µl FEP tubing (500 x 2.5 x 1.5 mm)
1600 µl FEP tubing (1000 x 2.5 x 1.5 mm)
2800 µl FEP tubing (1000 x 3 x 2 mm)
1.1 ml coiled FEP tubing (2200 x 1.6 x 0.8 mm)
3 ml coiled FEP tubing (1.5 mm i.d.)
5 ml coiled FEP tubing (1.5 mm i.d)
10 ml coiled FEP tubing (1.5 mm i.d.)
709910406
638306512
709910406
638306512
Cable kit for electrical connections (4 bare-ended wires 1.7 m)
6-pin terminal block for electrical connection
reference 290410 ; 4 heightening legs, 65 mm)
2704471M
2704473
13040103
27044307
27044317
27044337
27044347
2954662
2954663
2954682
2954668
2954674
2707253
2707255
Appendix A-4
27044307
27044317
27044337
27044347
2954662
2954663
2954682
2954668
2954674
2707253
2707255
Rack holding bar for one thermostating cuvette
Rack holder kit for thermostating cuvettes and series 30
polypropylene racks
Guiding bar for rack code 1
30A rack cover, stainless steel, 60 holes
31A rack cover, stainless steel, 108 holes
33A rack cover, stainless steel, 14 holes
34A rack cover, stainless steel, 36 holes
4 x 250 ml polypropylene solvent bottles
2 x 180 ml glass solvent bottles
4 glass bottle centering clips
24 polyethylene cap assemblies for solvent bottles
FEP injection port seal
Polypropylene rinsing well (85 mm)
Rinsing station plug
Parts & Accessories Lists
Appendix
Part Number
270430
12040302
12240101
2707401
270438
270439
130411
130411K
Code Material Max Nb Capacity and type of vials or tubes
0
0
1
7
8
9
10
10
PP
PP
PP
POM
PP
PP
PP
PVDF
80
80
80
96
120
120
27
27
2 ml glass vial
1.5 ml Eppendorf plastic vial, 11 x 40 mm without lids
9 ml glass tube, 13 x 100 mm
Standard disposable microtitration plate
0.3 ml glass vial
0.7 ml glass vial
5.5 ml glass tube, 12 x 65 mm
5.5 ml glass tube, 12 x 65 mm
150425
150422
150424
150498
20
21
22, 92
22U
PP
PP
PP
PP
108
60
44
44
150426
150427
150420
150429
23
24
28
29
PP
PP
PP
PP
44
14
108
60
4.5 ml glass tube, 10 x 100 mm
9 ml glass tube, 13 x 100 mm
32 or 25 ml glass tube, 18 x 180* or 150 mm
Variable size tube: 10 mm to 18* mm diameter,
stainless steel, 100* mm to 180 mm height.
8 ml glass or plastic scintillation minivial,17 x 65 mm
20 ml glass or plastic scintillation vial, 28 x 60 mm
3 or 3.5 ml glass tube, 10 x 65 or 75* mm
5.5 ml glass tube, 12 x 65 mm, 6 ml, 12 x 75* mm
2704430
2704530P
2704431
2704531P
2704432
30
30
31
31
32
Al
PP
Al
PP
Al
60
60
108
108
60
2704433
2704533P
2704434
2704534P
33, 93
33
34, 94
34
Al
PP
Al
PP
14
14
36
36
2 ml glass or plastic scintillation vial, 12 x 32 mm
2 ml glass or plastic scintillation vial, 12 x 32 mm
0.7 ml minivial, 7 x 40 mm
0.7 ml minivial, 7 x 40 mm
5.5 ml glass tube, 12 x 65* mm, 6 ml, 13 x 75 mm or
9 ml, 13 x 100 mm
20 ml glass or plastic scintillation vial, 28 x 60 mm
20 ml glass or plastic scintillation vial, 28 x 60 mm
4 ml vial, 15 x 45 mm
4 ml vial, 15 x 45 mm
2954651
2954715
60
61
SS
SS
4
4
250 ml polypropylene or 180 ml glass solvent bottle
250 ml polypropylene or 180 ml glass solvent bottle
2704599
--
PP
2704499
--
Al
Gilson Sample Racks
Gilson Sample Racks
A
variable variable, unperforated free rack for use with PC
software
variable variable, unperforated thermostating free rack for
use with PC software
PP= Polypropylene;
Al= Aluminum;
PVDF= Polyvinylydene fluoride;
POM= Polyacetal
* Only this size of tube can be used with level detection.
1) Racks of codes 20, 21, 28 and 29 are equipped to receive the needle wiper.
2) Racks of codes 92, 93 and 94 for the 222 model only are physically identical to racks of codes 22,
33 and 34 respectively. These codes are used to define the position in which the rack is to be
used. Codes 22, 33 and 34 are for the normal position and 92, 93 and 94 are for the lower
position when used with a 183 mm stroke arm and heightening kit.
Appendix A-5
Gilson Sample Racks
Parts & Accessories Lists
Code 0 rack
270430
Code 7 rack
2707401
Appendix
Code 0 rack
12040302
Code 8 rack
270438
Code 1 rack
12240101
Code 9 rack
270439
Code 10 rack
(13 + 14 = 27)
130411
130411K
Code 20 rack
150425
Code 21 rack
150422
Code 22 rack
150424
Code 23 rack
150426
Code 24 rack
150427
Code 28 rack
150420
Code 29 rack
150429
Appendix A-6
A
Parts & Accessories Lists
Appendix
A
Gilson Sample Racks
Code 30 rack
(4 x 15 = 60)
2704430
Code 31 rack
(6 x 18 = 108)
2704431
Code 32 rack
(4 x 15 = 60)
2704432
Code 33 rack
(2 x 7 = 14)
2704433
Code 34 rack
(3 x 12 = 36)
2704434
Unperforated
Aluminium rack
2704499
Code 30 polypropylene rack
(4 x 15 = 60)
2704530P
Code 31 polypropylene rack
(6 x 18 = 108)
2704531P
Code 33 polypropylene rack
(2 x 7 = 14)
2704533P
Code 34 polypropylene rack
(3 x 12 = 36)
2704534P
Unperforated polypropylene rack
2704599
Code 60 solvent rack
2954651
Code 61 solvent rack
2954715
Appendix A-7
Gilson Sample Racks
Parts & Accessories Lists
Appendix
Left-hand tray support
for small sampler
A
Right-hand tray support
for small sampler
2749666
Code 80
Microtitration plate tray
2749662
Tray supports for large sampler
2749893
Gilson Racks and Required Accessories
221 Model
Rack
code
Part Number
Accessories required for rack use
0
270430
12040302
Rack holding bar (130742) for rack series 0 to 10
H
1
12240101
Guiding bar (13040103)
N
7
2707401**
Rack holding bar (130742) for racks series 0 to 10
H
8
270438
Rack holding bar (130742) for racks series 0 to 10
H
9
270439
Rack holding bar (130742) for racks series 0 to 10
H
10
130411
130411K
Rack holding bar (130742) for racks series 0 to 10
H
* H: higher position
N: normal position (PP tray)
**: 2707401 plate holder only.
Appendix A-8
Position*
Parts & Accessories Lists
Part Number
20
21
22
22U
23
24
28
29
150425
150422
150424
150498
150426
150427
150420
150429
30A
31A
32A
33A
34A
2704430
2704431
2704432
2704433
2704434
30P
31P
33P
34P
2704530P
2704531P
2704533P
2704534P
Accessories required for rack use
Position*
N
N
N
N
N
N
N
N
Rack holding bar (2704471M) and
one thermostating cuvette (2704429)
N
Rack holding bar (2704471M)
H
62
2954962
Rack holding bar (2704471M)
H
80
2749662
Tray supports (2749666) for small sampler
H
Free P 2704599
Free rack, polypropylene, unperforated, for use with
PC softwares only, no code.
Free A 2704499
Free rack, aluminum for thermostating cuvette,
unperforated, for use with PC softwares only, no code.
Free 20 150499
Free series 20 tube rack, polypropylene, unperforated
for use in polypropylene tray with PC software only.
A
Gilson Sample Racks
Rack
code
Appendix
N
* H: higher position
N: normal position (PP tray)
**: 2707401 plate holder only.
Appendix A-9
Gilson Sample Racks
Parts & Accessories Lists
Appendix
A
222 Model
Rack
code
0
7
8
9
10
Part Number
Accessories required for rack use
270430
12040302
2707401**
270438
270439
130411
130411K
Rack holding bar (130742) for racks series 0 to 10
20
21
22
22U
23
24
28
29
150425
150422
150424
150498
150426
150427
150420
150429
30A
31A
32A
33A
34A
2704430
2704431
2704432
2704433
2704434
30P
31P
33P
34P
2704530P
2704531P
2704533P
2704534P
Position*
H
N
Rack holder kit (2704473) and
thermostating cuvette (2704429 or 2759550***)
H
Rack holder kit (2704473)
H
60
2954651
N
61
2954715
Heightening kit (2749870) (183 mm stroke vertical arm)
L
62
2954692
Rack holding bar (2704471M) for one thermostating cuvette
L
80
2749662
Tray supports (2749893) for large sampler
H
93
94
2704433
2704434
(33A) Heightening kit (2749870), thermostating cuvette*** L
(34A)
L
none
2704599
Free rack, polypropylene, unperforated for use with
PC softwares only as a custom rack, no code.
none
2704499
Free rack, aluminum for thermostating cuvette***, unperforated,
for use with PC softwares only as a custom rack, no code.
* H: higher position
N: Normal position (PP tray)
L: lower position (Heightening kit used)
**
2707401 microtitration plate holder only.
*** 2704429 is the fluid controller thermostating rack. 2759550 is the Peltier Effect thermostating
cuvette and must be used with the Gilson Model 832 Temperature Regulator reference 2759510.
Appendix A-10
Parts & Accessories Lists
FEP (polytetrafluoroethylene) tubing with two
end fittings to be connected to the injection
port:
Part Number
i.d.
(mm)
o.d.
(mm)
Length
(mm)
49964242
499671181
49963242
49943392
499625021
49962392
49948202
49948392
0.30
0.30
0.50
0.50
0.80
0.80
2.00
2.00
1.6
1.6
1.6
1.6
1.6
1.6
3.0
3.0
500
3000
600
1000
500
1000
500
1000
A
FEP Tubing
FEP Tubing
Appendix
Appendix A-11
Parts Lists for 221 and 222XL Generics
Parts & Accessories Lists
Appendix
A
Parts Lists for 221 and 222XL
Generics
221XL Generic and 222XL Generic liquid handlers
are similar to 221 and 222 as far as the basic structure
is concerned. Accessories have been adapted to
specific customer’s requirements, and therefore vary
from one machine to another.
Depending on the accessories, it is up to the user to
see whether any part of chapter 3 (installation)
applies.
Standard Equipment
Model 221XL
Model 222XL
Part Number Qty Part Number Description
27072001
F1410153
49967202
49967392
4701177592
1
1
1
1
1
27072001
F1410153
49967202
49967392
4701177592
Hydraulic
Needle tubing fitting
PVDF union connector for 1/4” - 28 TPI fittings
FEP tubing 500 x 2.5 x 1.5 mm, 800 µl
FEP tubing 1000 x 2.5 x 1.5 mm, 1600 µl
Rinsing station drain tubing, 1000 x 6.5 x 4 mm
54081252
1704731
3
1
54081252
15074002
Mechanical
Adhesive retainer for transfer tubing
Polypropylene tray (213 x 91.5 mm for 221) (393 x 244 mm for 222)
36078143
360831221
52903071
638306512
2749592
1
1
1
4
1
36078143
360831221
5290371
638306512
2749892
For the 183 mm stroke arm
GSIOC cable for 22X to model 401, 402 or Minipuls connection
RS232 cable for connecting sampler to PC (only for XL with 735)
Self-adhesive label for GSIOC identification
6-pin barrier strip connectors for electrical control socket
Liquid level detector cable (400 mm for 221, 480 mm for 222)
LT801205
1
LT801205
Documentation
User’s Guide
Appendix A-12
Parts & Accessories Lists
Appendix
Parts Lists for 221 and 222XL Generics
Accessories
Arms and needle related items
With 221 and 222XL Generics, there is the possibility
of replacing the standard 123 mm stroke vertical
arm with either a 56 mm or 183 mm stroke arm.
The 56 mm stroke arm is used for racks in the upper
position containing vials with septa.
The 183 mm stroke arm is for use with longer tubes.
Below is a list of the various accessories which can
be used in conjunction with each of the three arms:
Model 221XL Model 222XL
Part Number Part Number Description
270610
27067271
270610
27067271
27077113
27077113
27062634
27077213
27067272
27062634
27077213
27067272
27067212
27067212
27067276
27067277
27367513
27067276
27067277
27367513
27167215
27167215
270630
27067361
27067373
27067374
27067362
27367423
2749870
A
For the 56 mm stroke arm
56 mm stroke vertical arm
Bevelled edge constricting needle, for use with TransferPorts,
316L, 93.5 x 1.5 x 1.1 mm
Constricting needle, 316L, 93.5 x 1.5 x 1.1 mm
For the 123 mm stroke arm
Needle holder, polyamide (for bevelled edge needle)
Constricting needle 316L, 160.5 x 1.5 x 1.1 mm
Bevelled edge constricting needle, for use with TransferPorts,
316L, 160.5 x 1.5 x 1.1 mm
Side aperture needle for septum piercing and/or liquid mixing,
316L, 160.5 x 1.5 x 1.1 mm
Flat tipped needle for drop transfer, 316L, 165 x 0.7 x 0.4 mm
Needle sleeve for 27067276, 155 mm, flat tipped without “V”-cut
Teflon needle kit for applications where contact with metal is
undesirable (contains: Teflon needle, 157.5 x 0.9 i.d. ; Special needle
holder ; Needle guide)
Double needle kit (contains: Flat tipped needle, 181.5 x 1.5 x 1.1 mm ;
Triangular flat tipped needle, 169.5 x 2.5 x 1.1 mm ; PVDF connector ;
Special needle holder ; Needle guide)
For the 183 mm stroke arm
183 mm stroke vertical arm
Bevelled edge needle, 316L, 220.5 x 1.5 x 1.1 mm
Constricting needle, 316L, 220.5 x 1.5 x 1.1 mm
Bevelled edge constricting needle, for use with TransferPorts™,
316L, 220.5 x 1.5 x 1.1 mm
Side aperture needle, for septum piercing and/or liquid mixing
316L, 220.5 x 1.5 x 1.1 mm
Teflon needle for applications where contact with metal is
undesirable (contains: Teflon needle, 220.5 x 0.9 mm i.d. ; Special
needle holder ; Needle guide)
222 Heightening kit (contains: 4 heightening legs, 65 mm ; 2 tray rail
support bars)
Appendix A-13
Parts Lists for 221 and 222XL Generics
Parts & Accessories Lists
Appendix
A
Other Accessories
Model 221XL Model 222XL
Part Number Part Number Description
270230
21067720
2106773523
270230
21067720
2106773523
Sampler controller keypad with RS 232 coiled cable
Gilson 720 sampler keypad software package
735 Sampler Software
2749767
2949192
2954709
2749767
2954706
2954709
Injection port
TransferPort holding bar (221: 8 tubes, 222: 16 holes)
TransferPort accessory kit (contains: Polyamide transfer port
2800 µl, FEP tubing, 1000 x 3 x 2 mm, fitted with filter 316L, 40 µm ;
Sealing caps)
130742
Appendix A-14
2707404
2749872
Holding bar for racks series 0 to 10
Metallic front tray assembly (contains: Tray platform and arms
reference 290410 ; 4 heightening legs, 65 mm)
GSIOC
B
Appendix
GSIOC stands for Gilson Serial Input Output Channel. This communications channel
links all of the Gilson modules in a system together. The system controller (master)
controls all of the modules in a system by sending GSIOC commands to the slave modules.
Pumps and detectors are considered as slave modules in a Gilson system. Each device
connected to the GSIOC channel is distinguished by a GSIOC identification (ID) number
between 0 and 63. The GSIOC identification number is set by switches on each module
or by the module software for certain devices (for example, the 305 piston pump ID
number is set in the configuration menu). The system controller communicates with one
slave device at a time. GSIOC commands can also be used to control Gilson modules
directly from a PC.
The following are the default values (factory
settings) for samplers and pumps:
Instrument
If you have more than one instrument of the
same type, each instrument must have a
unique ID number.
All samplers*
All high pressure pumps**
401 Dilutors/402 Syringe Pumps
Minipuls 3
To change the ID number on an instrument,
refer to its User’s Guide.
ID Number
10
1
0
30
* All samplers = 221, 222, 231, 232, 233, ASPEC,
ASPEC XLi.
** Applies to 305, 306, 307, 308 Piston Pumps.
There are two types of commands: buffered commands and immediate commands.
Buffered commands are executed as background processes. They are used to send
commands.
Immediate commands have a higher priority; the execution of a buffered command will
be interrupted so that an immediate command can be executed. They are mainly used to
return messages from slave devices.
Appendix B-1
Immediate Commands
GSIOC
Appendix
B
Immediate Commands
Command
%
Description
Return the configuration of the sampler
Response format: mmmBVn.nn where
mmm is 221, 222, 231, 232 or 233
B indicates that the instrument is a version XL sampler
Vn.nn is the sampler version number
The identify has been loaded at final inspection in the non-volatile
memory using the buffered % command
$
Master reset
Response format: $ where
The returned $ indicates that the machine has been reset in its powerup state.
?
Mode status request
Response format: P where
P indicates that the instrument is operating in primitive mode.
For compatibility with version A samplers only.
B
Returns the contents of the command FIFO from the next command
to execute to the end of the FIFO (the maximum capacity of the FIFO being
900 characters.
Response format: The unexecuted contents of FIFO where
The FIFO can contain up to 900 characters, it is sents in segments. Each
immediate B command returns a segment. Each segment corresponds to a
buffered command previously sent to the sampler. As the commands are read
back, they are removed from the FIFO.
F
Flag computer -> sampler
determines if the sampler is waiting for a GSIOC command to another module or not.
Response format: uutc where
uu is the unit ID of the slave being interrogated.
t is the type of command expected, B for buffered, I for immediate
c is the letter of the command.
Example: 05ID
The sampler waits for an immediate command I sent to ID = 5.
If the sampler is not waiting, the string returned is ‘-’ (the minus sign).
G
Restarts the execution of the command queue
and returns G (if the command queue has already been paused by the
immediate H command)
Response format: G
H
Stops the execution of the command queue
Response format: H
Appendix B-2
GSIOC
I
Description
Read contact inputs
Response format: ccccc where
The first four parameters represent the four logic inputs, possible values 1
or 0 for each one. If the 5th input is switched to analog, 0 represents a
positive input.
Example: 10010
J
Returns the state of the conatact outputs
Response format: cccccccc where
The status of each output (1 to 8 respectively) is given. The first three
outputs are relay outputs, the next four are open collector outputs, and the
last is the front panel low pressure valve connector (0 = contact inactive,
1 = contact active). Each c is 0 or 1.
Example: 11101001
N
B
Immediate Commands
Command
Appendix
Returns the state and current sensitivity of the liquid level detector
Response format: Innn where
I represents the position of the needle (A for needle in air, L for needle
in liquid)
nnn is the current sensitivity setting, ranging from 0 to 255. The greater
the number, the lower the sensitivity.
Example: A10
S
Returns the sampler’s state
Response format: ab where
a is 0 if the command queue is empty, otherwise a = 1.
b is 0 if the queue is executed normally, 1 if paused or in error.
An error can be checked by using the immediate commands x, y, z and P.
A returned string of ‘00’ indicates the sampler’s at rest.
V
Read the absolute value of the analog input
Response format: decimal number in range 0 to 127 where
The value (0 - 127) represents the percentage of the full scale. Software
selectable as full scale value of 10 mV or 100 mV. Any voltage exceeding the
selected range is returned as 127.
This command is for compatibility with version A samplers only. See ‘v’.
X
Reads the X and Y coordinates
relative to the home position in units of 0.1 mm.
Response format: xxxx/yyyy where
The number of digits may vary according to the value. The letter R alone
is returned if the motors are running. If the position is negative, the
corresponding position returns ‘0’.
The home position is the position where the arm goes at power on. This position is set in
registers 33 and 34, and the value of these registers is subtracted from the coordinates in the
sampler’s work space as returned by the immediate commands x and y. If the value in any of
these registers is 65535, it is not subtracted and the value returned by the immediate x command
and that returned by one or both of the immediate commands x and y are equal.
In the small size XL sampler, the X-coordinate of any object in the work space is shifted to the
left by 5 mm relative to the version A sampler.
This command is for compatibility with version A samplers only, see ‘x’ and ‘y’.
Appendix B-3
Immediate Commands
GSIOC
Appendix
Command
Z
B
Description
Reads Z position relative to top position
Response format: zzzz where
zzzz is the distance from the top position in units of 0.1 mm. The number
of digits may vary according to the value. The letter R alone indicates that
the motor is running. If the position is negative with respect to the ‘home’
position, ‘0’ is returned.
This command is for compatibility with version A samplers only, see ‘z’.
@
Reads the value from one of the cells of non-volatile memory
These values include the home and full-scale positions for the three axes,
the analog input and various configuration parameters.
Response format: The value returned is a decimal number where
The address from which the value is taken is set by the buffered @ command.
Example: 203
f
Sets a flag in the sampler
so that after completion of the current buffered command, all other commands in
the FIFO are ignored and the contents of the FIFO are flushed.
Response format: f
If the current buffered command is F (freeze the interpretation of the buffer), this
forces the termination of the waiting as if the event had occurred. The immediate
command S will return ‘00’.
n
Returns the frequency of the liquid level detector oscillator
Response format: Frequency value in Hertz.
v
Returns the voltage at the analog input
Response format: sddddd:sddddd:sddddd where
ddddd is a decimal number in the range -1000 to +1000 representing the
voltage range -100 mV to +100 mV.
s is the appropriate sign + or -.
The three values are: the instantaneous value, the lowest value, and the
greatest value since the last reading.
Example: +00289:-00026:+00987
x, y or z
Returns the status of the relevant axis according to the format
Response format: Iddddd where
I can have the following values:
U, the axis is unpowered (free)
P, the axis is powered and steady,
R, the axis is moving,
E, the axis is in error (in this case it is unpowered).
ddddd is the actual position in 0.1 mm of the axis relevant to the ‘home’
position. If the position is negative relative to the home position, the format is
I-ddddd.
The error condition flag is reset each time a buffered command acts upon the
corresponding axis.
The coordinates are expressed in the sampler’s work space. Do not confuse with
the coordinates returned by the immediate X command that are compatible with
the convention used for version A.
Appendix B-4
GSIOC
Appendix
Command
F
Description
Freeze the interpretation of the buffer and interrogate the GSIOC commands
following along the bus until the specified command is sent to another slave.
Then resume the interpretation of the buffer and execute the next movement.
The typical use is to start a vertical motion when the dilutor is started, so as to
follow the displacement of the surface of the liquid.
Buffered Commands
Buffered Commands
B
Syntax: Fuutc where
uu is the unit ID of the slave to be interrogated.
t is the type of command, B for buffered, I for immediate.
c is the letter of the command.
Example F015D
I
Switches one of the valves according to the format
Syntax: In for the injection valve - this is not valid for 221 or 222,
In/ for the switching valve - use this syntax for 221 or 222, where
n is 0 to turn the valve into the Load position, and 1 to turn to the
Inject position.
In/ is the same as above but operates the switching valve.
This command does not proceed until any current the valve switching is
complete. If an error is detected, the pause flag is set and execution of the
queue is stopped. Only one valve can be switched at a time.
J
Sets the outputs and the low pressure valve
Syntax: Jabcdefgh where
a is 0 to energize the first relay, 1 to release it, or X to keep its current state
b and c have the same effect on the second and third relays respectively.
d is for the first open collector output.
e, f and g are the second, third and fourth open collector outputs
respectively.
Example: J00110001
This command cannot be truncated. If only the first relay must be operated, the
command J1XXXXXXX must be sent.
N
Sets the sensitivity of the liquide detector
Syntax: Nnnn where
nnn is a value from 0 to 255.
The smallest numbers give the highest sensitivity. However an excessive
sensitivity could produce undesired detections. If no value is supplied, the
sensitivity is unchanged. In both cases, the state of the detector is reset to ‘A’ (air).
This command must be followed by the sequence @* to make the change permanent.
Default value: 10
T
Wait until the specified time has elapsed.
Syntax: Tttttt where
ttttt is the delay ranging from 0 to 6000 in 1/100 ths of a second (6000 = 1 min).
If the value exceeds this limit, the command is ignored. The command with no
argument is the equivalent to the execution of the immediate H command: the
sampler waits until the immediate G command is sent.
Appendix B-5
Buffered Commands
GSIOC
Appendix
Command
B
Description
V
Sets the sensitivity of the analog input when interrogated
with the immediate V command.
Syntax: Vd where
d is either 0 or 1, setting the full-scale respectively to 10 mV or 100 mV.
The default value is set at delivery to 10 mV. The command must be followed
by the sequence @* to make this change permanent.
This command is for compatibility with version A samplers only.
W
Stops the execution of the queue until all three axes have reached
their destinations, or are stopped because an error has been detected.
In the case of an error being detected, the execution is paused and the pause
flag is set. The flag can be read back using the immediate S command.
X
Sends the arm to a pair of coordinates in the XY plane relative the
‘home’ position
Syntax: Xxxxx/yyyy where
xxxx is the x-coordinate in 0.1 mm
yyyy is the y-coordinate in 0.1 mm
0/0 is the home position.
The home position is the position where the arm goes at power on.
This position is set in registers 33 and 34, and the value of these registers is added
to the parameters supplied for the current command. If the value of any of these
registers is 65535, it is not added and the value to supply to the command X and
that supplied to either or both of the immediate commands x and y are equal.
In the small size XL sampler, the x-coordinate of any object in the work space is
shifted left by 5 mm relative to the version A sampler.
This command is for compatibility with version A samplers only.
Z
Sends the arm to the specified height
Syntax: Zzzzz is the coordinate in 0.1 mm, 0 is the top position.
This command is for compatibility with version A samplers only, see ‘A’.
@
Writes a value into one of the cells in the buffer of the non-volatile memory.
These values include the home and full scale positions for the three axes,
the analog input and various configuration parameters
Syntax: @aa or @aa=vvvv
For the command @aa,
aa is the decimal address of the cell: the command sets the pointer to this
cell for subsequent reading. THe address ranges from 0 to 54.
For the command @aa=vvvv,
aa is the address of the cell,
vvvv is the value assigned to it.
Each value ranges from 0 to 65535 except for the first five values that range
from 0 to 99 000 000. The values are changed in the EEPROM buffer only, the
change is temporary. The previous values will come back when the sampler is
reset with the immediate $ command or the next time the sampler is switched on.
If it is desired that the change is permanent, the command must be followed by
a ‘*’ that saves all the configuration parameters into the EEPROM at once.
Examples: @12* selects the cell 12 and saves the previous changes.
@12=458* writes the value 458 into the cell # 12 and saves the previous changes.
@* only, saves all the previous changes.
The function of the 34 pre-assigned memory cells is given in the table in the 719
User’s Guide, Appendix C.
Appendix B-6
GSIOC
v
Description
Sets the speedalong the specified axis (X, Y or Z)
Syntax: vaxxxx where
a is the axis name (X, Y or Z), capital letter.
xxxx is the speed in 0.1 mm/sec.
If the buffered command @* is sent before the sampler is switched off, this
speed becomes the default value at the next power on.
Value ranges: X and Y = from 1000 to 2500, Z = from 1 to 1250
Default values: X and Y = 2500? Z = 1250
z
B
Buffered Commands
Command
Appendix
Allows individual axis control
Syntax: zcnnnn where
c is the axis letter, X, Y or Z
nnnn is the absolute coordinate in 0.1 mm: 0 is the home position for X and
Y, and the top position for Z.
If the value is preceded by a + or a - sign, the coordinate is considered relative to
the current position, that is z+10 means ‘lower the needle by 1 mm’.
A variant clnnnn where l is the lowercase ‘L’ letter and allows a travel to be
stopped if the liquid level detector senses the liquid before the specified position
has been reached. The actual position can then be read back by sending the
corresponding immediate command (x, y or z).
If the coordinate is replaced by a star (*), the corresponding axis is unpowered
(the motor is free). Sending a command with no coordinate at all (X, Y or Z
alone) reconnects the motor without moving it. It is also reconnected by any of
the motion commands that affect the corresponding axis.
Appendix B-7
GSIOC
Appendix B-8
Appendix
B
Technical Data
Appendix
C
This chapter contains the Technical Data concerning the 221/222 Liquid Handlers.
Application: Sample preparation (dilution, serial dilution, addition of reagents and
solvents, any liquid transfer from tube to tube, mixing), analyzer loader.
Appendix C-1
Hydraulics
Technical Data
Appendix
C
Hydraulics
Vial Capacity
From 120 microvials of 0.3 ml for the 221XL or 540
microvials of 0.7 ml for the 222XL up to 4 bottles of
250 ml.
Choice of 25 standard Gilson racks, including 5
thermostated racks, plus special racks, a bar that can
hold up to 8 TransferPorts for the 221XL or 15
TransferPorts for the 222XL, and free vessel
programing.
Syringe Capacity (402 Syringe Pump)
0.1, 0.25, 0.5, 1, 5, 10 and 25 ml.
Liquid Flow Rate (402 Syringe Pump)
From 0.01 to 120 ml/min., continuously.
Liquid-contact Materials
316L stainless steel or titanium, FEP, PTFE, PEEK,
and PP.
Needle Gas-supply
Through 3-port valve (additional accessory) for
evaporating, degassing and mixing.
Sample Cross-contamination
Lower than 1 ppm, with liquid level detector and
recommended values for rinsing parameters (4 mm
immersion, 20 ml/min., 1 ml and 2 ml for needle
inside and outside respectively), and lower than 50
ppm without liquid level detection.
Appendix C-2
Technical Data
Arm Motion Speed
C
Mechanics
Mechanics
Appendix
250 mm/s horizontal, 125 mm/s upwards and 90
mm/s downwards.
Needle Tip Positioning
± 0.2 mm reproducibility and ± 0.75 mm accuracy in
each direction.
Appendix C-3
Electrical
Technical Data
Appendix
C
Electrical
Remote control
RS-232C and GSIOC.
Inputs
4 electrical contacts and one analog signal (100 mV),
convertible as a 5th contact.
Outputs
7 electrical contacts including 3 relays, plus two
power supplies for 3-port valve and other auxiliary
equipment (12 VDC, 500 mA).
Appendix C-4
Technical Data
Gilson Sampler Controller Keypad via
RS-232C
C
Control
Control
Appendix
8-line, 40-character display, 24 keys, with Gilson 720
Keypad Software (3.5", double-sided, high-density,
1.44 MB diskette).
Microcomputer via RS-232C or via
GSIOC through Gilson 506 Interface
Gilson 719 PC Software: DOS 3.1 or higher,
Microsoft Windows 3.1 or higher, 4 MB RAM.
Gilson 735 Sampler Software: 120 MHz Pentium®
Processor, 32 MB RAM, Windows® 95 or NT® 4.0, 50
MB hard disk space.
Appendix C-5
Programs
Technical Data
Appendix
C
Programs
Automated Laboratory Tasks Available
Add, aliquot, calibrate, dilute, dilute and add, dilute
serially, dispense, mix, move arm, pipette, rinse
needle, set electrical contact and wait.
Size
Unlimited (more than 1MB available on 720
diskette).
Storage
Permanent (for more than 100 programs on 720
diskette).
Appendix C-6
Technical Data
Storage
Indoor use only.
Installation: Category II.
Altitude: Up to 2000 m.
Temperature range: 5°C to 40°C.
C
Environmental Conditions
Environmental Conditions
Appendix
Pollution degree 2.
Humidity: Maximum relative humidity 80% for
temperatures up to 31°C decreasing linearly to 50%
relative humidity at 40°C.
Power Requirements
Frequency: 50 to 60 HZ.
Voltage: 100 to 240 V ac, mains supply voltage
fluctuations should not exceed ±10% of the nominal
voltage.
Power rating: 110 VA.
Each XL instruments is subjected to a Dielectric Test
(1500 V between live/neutral and earth) and an
Earth Bonding Impedance Test (압0.1 ⏲).
Size and Weight
221XL: (W x D x H) 330 x 465 x 230 mm, 12.2 kg.
222XL: (W x D x H) 515 x 620 x 230 mm, 16.2 kg.
Appendix C-7
Technical Data
Appendix C-8
Appendix
C
Reference Information
Appendix
D
This chapter contains two tables, one indicates which liquids are acceptable for use with
different materials. The second table indicates which racks are compatible with the
different sampler models.
Appendix D-1
Chemical Inertness of Plastics Used
Reference Information
Appendix
D
Chemical Inertness of Plastics Used
Plastics used are:
- Polytetrafluoroethylene (FEP),
- Polypropylene (PP),
- Polyvinylidene fluoride (PVDF).
FEP has a high chemical inertness to common
liquids. PP and PVDF have a high chemical inertness
to aqueous, acid and basic solutions, at ambient
temperature:
Organic solvent
where:
Appendix D-2
+ for acceptable
0 for exposure
- for unacceptable
Acetic acid (glacial)
Acetone
Acentronitrile
Benzene
Carbon tetrachloride
Chloroform
Cyclohexane
Dioxane
Diethylether
Ethyl acetate
Heptane
Hexane
Ketones (general)
Methanol
Methylene chloride
Tetrahydrofuran (THF)
Toluene
Trichloroethylene
b.p. in °C
PP
PVDF
118
56
82
80
78
61
81
101
35
77
98
69
+
+
+
+
+
+
+
0
+
+
+
0
0
0
0
+
+
+
+
+
+
0
+
+
+
+
+
+
+
0
+
+
65
40
66
111
87
Aluminum
needs thermostating cuvette
Polypropylene
Tube Racks
Free
Racks
0
1
7
8
9
10
20
21
22
22U
23
24
28
29
30
31
33
34
30
31
32
33
34
60
61
62
92
93
94
F
polypropylene
aluminum
aluminum free rack
series 20 free rack
tubes 압 100 mm long
polypropylene
aluminum
aluminum
series 20
Fitted with heightening kit (2749870),
polypropylene tray on worktop and
183 mm Z-arm
Fitted with heightening kit (2749872),
platform in LOW position, polypropylene
tray and 183 mm Z-arm
Fitted with heightening kit (2749872),
platform in HIGH position, polypropylene
tray and 123 mm Z-arm
221XL
Fitted with metallic holding bar
(2704473)
Fitted with rack holding bar for one rack
(2707404)
Standard configuration:
Polypropylene tray at worktop level
and 123 mm Z-arm
Standard configuration:
Fitted with Polypropylene rack tray and
123 mm Z-arm
Fitted with small support arms
(130742) and 123 mm Z-arm
123 mm Z-arm and rack holding bar
(2704471M)
Rack Compatibility Table
222XL
Rack Compatibility Table
Bottle
Reference Information
Appendix
D
guiding bar
13040103
tubes 압
tubes up to
tubes up to
100 mm long 150 mm long 180 mm long
rack heightener
2954694
Polypropylene free rack
with rack heightener
Appendix D-3
Reference Information
Appendix D-4
Appendix
D
World Wide Web: www.gilson.com
E-mail: sales@gilson.com, service@gilson.com, training@gilson.com
World Headquarters
Gilson, Inc.
3000 W. Beltline Hwy., P.O. Box 620027, Middleton, WI 53562-0027, USA
Telephone: (1) 800-445-7661 or (1) 608-836-1551 • Fax: (1) 608-831-4451
Gilson SAS
19 avenue des Entrepreneurs, B.P. 145
95400 Villiers-le-Bel, France
Telephone: (33) 1-34-29-50-00 • Fax: (33) 1-34-29-50-20
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