Instruction Manual for the
Revolutionary Science RS-IC-100
IncuCount
R E V O L U T IO N A R Y S C IE N C E
Ma nufa ct ur er o f P r ecis io n Labo r at o r y Eq uip m e nt
Table of Contents
Introduction
Parts Checklist, Notes, Service Record
Warranty
Safeguards and Cautions, Limitations of Use
Intended Use
Specifications, Contact Information
Hardware Installation
Software Installation
Setup – Lighting, Focusing, Brightness and Contrast Controls
Light Control, Light Aperture, Focusing, Brightness and Contrast Controls
Creating a New Project
Capturing Images
Colony Counting
Counting Multiple Colony Types, Advanced Image View, Sensitivity and Size,
Centering Countable Area
Sequential Imaging, Growing Bacteria Upside Down
Advanced Features
Make Movie (animate), Preview Movie, Resetting the Image,
Zoom, Live Video Feed
Selecting a Previous Image
Making Notes
Saving a Project
Opening a Saved Project
Importing Images
Export to Excel
Tools Menu
Zoom In, Zoom Out, Center, Exclusion Zone, Antibiogram,
Email Setup, Video Setup
Antibiotic Reactions – The Measurement of Inhibition Zones
View
Outline CFUs, List View, Image View
Help
Appendix A-Color Space
Appendix B-Camera Settings
REVOL UTIONARY SCI ENCE
17319 Lake Blvd.
Shafer, MN 55074
Toll Free: (800) 775-7942
Fax: (775) 218-7015
E-mail: support@revsci.com
Website: www.revsci.com
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IncuCount RS-IC-100
Introduction
Dear Valued Customer,
Thank you for purchasing the Revolutionary Science RS-IC-100 IncuCount. We are
confident that when used correctly, our IncuCount will provide years of reliable
service. We have made every effort to use high quality, durable parts to deliver the
performance expected of instruments costing 2 to 3 times as much.
You may enjoy visiting our web site at www.revsci.com. Please let us know what you
think of our products, positive or negative, so we can continue to enhance and
improve our products in the future.
Sincerely yours,
REVOLUTIONARY SCIENCE
NOTE: This manual contains important information, safeguards and operating
instructions for the operation of the REVOLUTIONARY SCIENCE model RS-IC-100
IncuCount by Alternative Pioneering Research & Development, Inc. (APR&D, Inc.)
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IncuCount RS-IC-100
Parts Checklist
Part
Model number
_ IncuCount
200-A00001
_ Carrying case
100-A00002
_ Light aperture
200-A00003
_ Petri dish holder
_ Software (1 CD ROM)
100-A00004
_ Manual
310-A00001
_ Detachable power supply
Notes & Service Record
For your own future reference and to expedite answers to your questions, please
complete the following information:
Date Purchased _________________________________________
Serial Number __________________________________________
Model Number __________________________________________
Purchased From _________________________________________
Your Notes:
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IncuCount RS-IC-100
Warranty
Warranty Registration
Please fill out this page, or a copy of it, and send it to the address below to validate
your warranty. Warranty Registration Card for the REVOLUTIONARY SCIENCE RS
-IC-100 IncuCount. (Please Print)
Date Purchased: ___________ Serial Number:__________________
Purchased From: _________________________________________
(Please check one) ____ Company ____ School ____ Individual
Name of Department or Institution:
_______________________________________________________________
__________________________________________
Address:
_______________________________________________________________
_______________________________________________________________
______________________
Intended use:
_______________________________________________________________
Where did you hear of our product?
_______________________________________________________________
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Comments:
_______________________________________________________________
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Warranty Policy
Your REVOLUTIONARY SCIENCE RS-IC-100 IncuCount is warranted to be free
from defects of material or workmanship under normal use for a period of two
years from date of purchase. If your RS-IC-100 IncuCount proves to be defective
within this warranty period, APR&D, Inc. will repair or replace your unit at our
option. This warranty will be void if malfunction is caused by accident, misuse, or
negligence, including tampering, abuse, or damage in transit. This warranty excludes
any relief for incidental or consequential damages.
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IncuCount RS-IC-100
Safeguards & Cautions
To maximize the use of the IncuCount, please follow the
recommendations in this short list before using:
1. Examine carton and contents for possible damage to the product caused
during shipping. If damage is present contact carrier at once. Failure to do so
immediately may release the carrier from any liability of damage.
2. Fill out the checklist (page of instruction manual). If something is missing
please contact us.
3. Carefully read this instruction manual before operating the IncuCount.
4. Fill out warranty registration card and mail it to APR&D, Inc.
Limitations of Use
*Revolutionary Science is not responsible for misuse of the IncuCount 100
1. ALWAYS pre-clean the IncuCount light panel of residue after use with a damp
cloth and appropriate mild detergent solution. Never clean IncuCount with
organic solvents like thinner or benzene. It will damage the surfaces.
CAUTION: This is the only recommended method of cleaning.
2. Never attempt to dismantle or modify the IncuCount.
3. Never operate the IncuCount with a damaged USB cord or after the appliance
malfunctions or has been damaged in any manner. THIS MAY CAUSE DAMAGE
TO YOUR COMPUTER.
4. Accessory attachments not recommended by Revolutionary Science may
damage to the IncuCount or the computer.
5. Never place the IncuCount or any part of the IncuCount in an environment
that exceeds 65 degrees Celsius or below 5 degrees Celsius. Humidity should be
at or below 65%.
6. Never use IncuCount for any purpose other than the intended use.
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7. When used at high temperatures, the IncuCount may be uncomfortable to
the touch. When removing the IncuCount from the incubator, it is
recommended to use temperature resistant gloves or turn off the incubator and
allow the IncuCount to cool to a comfortable temperature before removing it.
8. Never attempt to modify the plugs or cords. This may cause damage to the
IncuCount or your computer.
9. Never immerse the IncuCount, cord, or plug in water or operate on wet
surfaces.
10. Never allow the cords to contact hot surfaces beyond 65°C. Any slack in
the cord should be wrapped up and held together with a band.
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Intended Use
IncuCount RS-IC-100
The Revolutionary Science IncuCount 100 can be used for the following
purposes:
1. Colony Counting:
·
Count colonies on Petri dishes
2. Measure the inhibition zones of antibiotic samples
·
Monitor the inhibition zones in an incubated or refrigerated environment.
3. Monitor inside an incubated environment
·
Count colonies as they grow in real time
·
Measure inhibition zones as anti-biotic reactions occur in real time
4. Animate
·
Create an animation of any Petri dish reaction, such as colony growth or
inhibition zones.
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Specifications
Model No.:
Power requirements:
Net weight:
Dimensions:
Lights:
Camera:
Computer interface:
Petri dish size capacity:
CE/UL Certifications:
RS-IC-100
100 -240 VAC 60 HZ 1.5 amps
5 lb. (shipping weight 6 lb.)
7.25 inches tall x 6.25 inches diameter
High intensity LED lights, top and bottom
16 megapixel digital camera
USB
Up to 100 mm in size, including 3M Petri Film
UL approved LED lights and power supply
System Requirements
Hardware:
Memory:
Operating System:
Hard drive space:
Warranty:
Computer, CD ROM, USB interface
Min 512MB, 500mhz
Windows XP, or higher
Min 150MB
2 year, repair or replacement
Contact Information
Revolutionary Science
17319 Lake Blvd
Shafer, Minnesota 55074, USA
Customer Service:
1(800) 775-7942
Technical Assistance:
(651) 674-3112
Fax:
(775) 218-7015
Web site: www.revsci.com
E-mail: support@revsci.com
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IncuCount RS-IC-100
Hardware Installation
Note: Hardware MUST BE INSTALLED before software is installed.
1. Place IncuCount on a dry, level surface, note: whether inside or outside an
incubated or refrigerated environment it does not matter.
2. Plug USB cord into a nearby computer.
Illustration 1: USB Cord
3. Plug the power adapter into the wall outlet (100 to 240VAC)
Illustration 2: Power Adapter
4. Insert the barrel connector to the power socket at the rear of the unit.
Illustration 3: Barrel Connector
5. If the IncuCount is placed inside an incubator or refrigerator, the door can
shut on the USB cord and power cord as long as it does not damage
them.
Note: Software will install, but will not open if the IncuCount is not hooked up
properly.
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Software Installation
Note: Hardware MUST BE CONNECTED before software is to be
installed. Please also note: after software installation you will be
prompted to restart your computer to complete the process.
1. Insert IncuCount installation CD ROM in the computer. The following
window should show up on your screen. If the window does not show,
click on the IncuCount Installer Icon. Click 'NEXT.'
Illustration 4: Setup Options
2. Choose a destination folder and click 'INSTALL.’
Illustration 5: Choose Instillation Folder
3. Installation software will then copy all necessary files to your computer.
The installation software will alert you when it is complete. The driver
setup program will then run to install the IncuCount camera driver. If
the IncuCount camera is not yet connected to a designated USB port, do
so now.
Illustration 6: Camera Driver Setup
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4. Select language. Click ‘OK.’
Illustration 7: Choose a Language
5. Click ‘INSTALL.’
Illustration 8: Installing Camera
6. When the driver setup is complete, you will be prompted to reset your
computer.
Illustration 9: Restart Computer
If you have difficulty installing the software or connecting the hardware,
please contact Revolutionary Science Technical Support.
Note: There is no charge for contacting Technical Support.
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Setup – Lighting, Focusing, Brightness and Contrast Controls
Light Control
The IncuCount comes equipped with an upper and a lower bank of LED lights. To
turn on/off these lights, flip ‘ON/OFF’ the rocker switches located in the back of
the unit (see Illustration 10). (As viewed from the back, camera end up) the left
switch controls the lower bank of lights and the right switch operates the upper
bank.
Illustration 10: Switches for Top and Bottom Lights
To test the lights, run the IncuCount software and click the ‘LIVEFEED’ button
(see Illustration 11) in the gray control panel (also located in the View menu under
Live Video Feed). The lights should activate (depending on which rocker switch is
on). Adjust according to preference and agar color (See Illustration 12).
Illustration 11: Live Feed Button
Illustration 12: Blood Agar Plate with Bottom Light On
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Light Aperture
Place the light aperture (black ring, Illustration 13) into the unit on the light box.
Note: The purpose of this important mechanism is two fold.
Centers the Petri dish in the camera's field of view
Blocks out excess light around the Petri dish, providing the best possible view
of the sample.
Illustration 13: Light Aperture
Focusing
Run the IncuCount software and open up
the live video feed by clicking on the LiveFeed button (see Illustration 11, page 12).
Open the camera control panel by
left-clicking on Video Setup in the Tools
menu. While viewing the live video feed,
position a Petri dish inside the black aperture (see Illustration 12, page 12). Move
the focus slider to the right or left until
Petri dish is in focus. Because of different
agar depths, this may need to be done, but
rarely. Close Live Video Feed window
when the Petri plate is in focus. Change
the Exposure and Gain sliders to achieve
the desired brightness. Turn on Auto
Controls if desired for auto focus and auto
exposure.
Illustration 14: Camera Settings
to Adjust Focus, Exposure, etc.
Brightness and Contrast Controls
To change brightness or contrast bring up the webcam properties page and left
click of the Advanced Settings Tab (see Illustration 14). Move the Exposure, Gain,
Brightness, Contrast and Color Intensity sliders to the right or left to get the best
picture. (See Appendix B for an explanation on the camera controls)
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IncuCount RS-IC-100
Creating a New Project
1.To create a new project, click on ’NEW PROJECT’ (see Illustration 15) in the file
menu.
Illustration 15: Creating a New Project
2.A file dialog box will pop up (see Illustration 16) asking you to create a new
folder for the project. This will be the folder where captured images are stored as
well as data files. Type in a meaningful title for the folder and click ‘OPEN.' You
are now ready to start capturing images.
Illustration 16: Create Project Folder
Capturing Images
To capture an image for counting or measuring an inhibition zone, click the
’CAPTURE’ button in the gray control panel (the control panel will look like
Illustration 18 or 19, depending on whether the Colony or the Antibiotic button is
selected). If you haven't yet created a new project, you will now be prompted to
do so (see Creating a New Project section on this page).
Illustration 17: Capture-Counted Colonies
Illustration 18: Capture-Inhibition Zones
The lights on the IncuCount should flash (see Setup section, page 9) and the image
of the Petri dish inside the IncuCount should appear on the screen. Before counting, you may need to adjust the sensitivity. Before capturing, you may need to
adjust the focus and lighting.
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IncuCount RS-IC-100
Colony Counting
To count an image, simply click the ‘COUNT’ button on the control panel. The
Colony-Forming Unit (CFU) count will appear in the Type C text field (see
Illustration 19). Dark regions judged too small (see Sensitivity and Size section on
page 17) will appear in the Invalid text field. If you are counting more than a single
type of bacterium (see Counting Multiple Colony Types, this page), the CFU count
of each will appear in the Type A, Type B, or Type C text field.
Illustration 19: Colonies of Type C and
Invalid Appear in Corresponding Text Fields
In the Image Panel, colonies will be colored green, while those too small (see
Sensitivity and Size section on page 17) appear as red (see Illustration 20).
Illustration 20: Counted Colonies Appear in
Green, Uncounted (too small) Appear in Red
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Counting Multiple Colony Types
To count multiple colony types, open the Advanced Image View by clicking on the
‘ADVNACED’ button (Illustration 21) in the Control Panel.
Illustration 21: Click on Advanced Button to
Open Advanced Image Window
The Advanced Image View panel (Illustration 22) will open up. You can choose
the color of your colonies by selecting Type A through Type J, then left clicking
again on a representative colony. This color will show in color box next to ‘Select
Bacteria Color:’. Color is represented by 3 numbers (one each for red, green and
blue, see explanation of color space in Appendix A, page 29) ranging from 0 to
255. You can change the tolerance (measure of how close a color is to your representative color) by typing in a number between 0 and 255.
Illustration 22: Advanced Image View Panel
Alternatively, you can set the Agar color by clicking the ‘AGAR SELECT’ button
(see Illustration 23). This will allow you to choose which color will not be
counted.
Illustration 23: Select Agar Color
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Sensitivity and Size
Counting is performed by scanning for dark regions on the image of the Petri
plate.
You can set the minimum size of a counted CFU (measured in pixels). Counted
CFUs show up green while uncounted ones show up red. Adjust the Minimum
Size slider to give you the best count.
Illustration 24: Minimum Size Slider
You can adjust the sensitivity (measure of how dark a
region needs to be in order to be counted) by either
typing in a value between 0-255 in the sensitivity field
or moving the sensitivity slider. Adjust the slider to
give you the best count.
Illustration 25: Sensitivity Slider
Centering Countable Area
While adjusting the size and sensitivity, you may have noticed a red circle drawn
around the inside of the image of the Petri dish. Dark regions inside this circle will
be counted, while dark regions outside of the circle will not be counted. To
change the radius or position
of this circle, click Center in the
Tools menu.
Illustration 26: Center Tool
To change position of the counting area (see Illustration 27), position the mouse
where the new center should be and left click on the mouse. To change the
radius (see Illustration 28), position the mouse on the outer edge of the counted
area and right click on the mouse. If a spiral plate is being counted, left-clicking
while holding down the 'Shift' key will make a concentric circle appear (see
Illustration 29) on screen. The area within this circle will not be counted. Holding
down the 'Shift' key and right clicking the mouse will remove this circle.
Illustration 27: Center the
Counting Area
Illustration 28: Resizing the
Counting Area
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Illustration 29: Concentric
Circle for Spiral Plate
Counting
IncuCount RS-IC-100
Sequential Imaging
Colony counting is the default mode. In the gray panel to the right (see software
image below), are controls for Sequential Imaging and Colony Counting use.
Sequential Imaging means capturing and counting Petri plate images at set intervals
for a specified time.
To set times, enter the time that the IncuCount should begin capturing images
into the Start Time field. All time is measured on a 24hr/day clock, (for
example, 13:23:59 would be 1pm, 23 minutes, and 59 seconds).
Enter starting date in the Start Date field. All dates must be in the format
“MM/DD/YYYY”. Incorrect formatting will result in the data field being
replaced with the current day in the correct format.
Set the Stop Time and date just as you did the Start Time and date. Enter in
the interval time (time between image captures) into Interval time field.
Interval time is recorded in minutes (default is 20 minutes).
Press the ‘START’ button to start automatic capture. If any field is left blank
or has an incorrect format, it will be corrected to the current time/date
(or a day later for the stop time/date).
If you haven't created a new project you will be prompted to do so now (see
Creating a New Project, page 14). Images will then be captured (see Illustration
30) and counted at the appropriate times. Information about the current image is
shown in the center text column.
Illustration 30: Captured Image.
Growing Bacteria Upside Down
It is recommended that most Petri plates inoculated with bacteria be grown upside
down. This can be done by using the retaining clip (included) to hold the Petri dish
in place and then simply turn the IncuCount upside down. The software will adapt
to the repositioning of the IncuCount.
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Advanced Features
Advanced Image View
To access advanced features of the IncuCount, open up the Advanced Image View
window by clicking on the Advanced button in the control panel (see Illustration
20, page 15). In addition to being able to select agar and colony color, the
Advanced Image View panel has 5 other buttons: Make Movie, Preview Movie,
Reset Image, Zoom, and Video Feed (see Illustration 32).
Illustration 31: Advanced Image View Buttons
Make Movie (animate)
Click on the ‘MAKE MOVIE’ button, you will be prompted to name your movie.
Movies may be created in .mov format only. Type in an appropriate name for your
movie and click save.
A window will request the frames per second (fps) of the animation (see I
Illustration 32). Enter in an appropriate value and click on ‘OK.’ Movies may be
viewed in an appropriate media viewer. Movies may also be viewed by clicking the
‘PREVIEW MOVIE’ button.
Illustration 32: Enter Movie Speed
Previewing a Movie
If you want to watch a movie of your Petri dish images, click on the ‘PREVIEW
MOVIE’ button in the Advanced Image View window. You then will be asked how
fast you want the movie to be played. Enter the speed and press ‘ENTER’ or click
‘OK.’ Your movie will be shown in the Advanced View's image panel.
Reset Image
After previewing a movie or zooming, you may have to reset the image on the
Advanced Image View. Press the ‘RESET IMAGE’ button. This will reset the image
shown in the image panel to the current image shown in the main panel.
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Zoom
To zoom in on a feature in the Advanced Image View's image panel, click the
‘ZOOM’ button then make a box around the feature (see Illustration 33) by moving the mouse and left clicking where you want the corners of the box to lie.
Illustration 33: Advanced Image Zoom
To close up the box, right click where you want the final corner to lie. A blown
-up image of your boxed in region will appear in the image panel (see Illustration
34).
Illustration 34: Zoomed in Image
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Live Video Feed
Click the ‘VIDEO FEED’ button to open the Live Video Feed panel. This panel is
used for focusing the camera (see Focusing, page 13). Close this panel when completed, else it may interfere with image capture.
Selecting a Previous Image
After capturing 2 or more images, select which image is currently shown in the
image panel by clicking on the icon of your image in the image chooser panel. This
icon will highlighted with a red box and the image will appear in the image panel.
The CFU count will appear above the image along with the image's name.
Illustration 35: Selecting a Previous Image
Making Notes
Notes can be made about any image by clicking in the Notes field (see Illustration
36) and typing whatever notes need to be made. These notes are associated with
the currently selected image.
In the image chooser panel, the note will be indicated above the icon of its
associated image.
Illustration 36: Making Notes
Saving a Project
Once the project is complete (or any time during), the project may be saved by
clicking on ‘SAVE’ in the File menu. This will save the project data file in the image
folder created previously.
Illustration 37: Saving a Project
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Opening a Saved Project
To open a previously saved project file, click on ‘OPEN PROJECT’ (Illustration 38)
in the File menu.
Illustration 38: Opening a Project
You will then be asked what file you want to open. Highlight the .dat file (see
Illustration 39) and click ‘OPEN.’
Your project will then load.
Illustration 39: Selecting a Project to Open
Importing Images
Images may be imported into a project by clicking on Import Image (see
Illustration 40) in the File Menu or Add Frame from the gray control panel. A
window will open up with the caption, “Importing Image:” (Illustration 41). Type
in the name of the desired image file. Click ‘OPEN’ and the image will show up on
the image panel.
Illustration 41: Select Image to be Imported into Project
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Illustration 42: Export to Spreadsheet
Illustration 43: Tools Menu
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Illustration 44: Creating an Exclusion Zone
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Email Setup
When Email Setup is selected from the Tools menu, a setup dialog (see Illustration
45) is loaded where information necessary to sending an email is stored. Email
Setup requires an Outgoing Server (smtp server), Port number, User Name and
Password, and a List of Recipients (separated by a comma):
Outgoing Server: This is the address of outgoing server
(for example smtp.gmail.com , for a gmail account),
refer to mail server's literature for more information
on configuring a smtp client.
Port-enter port number: (refer to mail server's
literature).
User Name and Password: This is the user's email
address or login name (refer to mail server's literature)
and associated password.
List of Recipients: Type in the full address of each
recipient of email messages separated by a comma.
Illustration 45: Email Setup
Selecting the Test button will send a user selected file to each of the recipients to
verify the information in the email setup is correct. Select the 'APPLY’ button to
save the setup information or exit to cancel. (Selecting the Test button will also
save setup data)
Video Setup
To change default video settings, left click ‘VIDEO SETUP’ in the tool bar. This
brings up the camera properties page. Adjust these settings for best picture quality (See Focusing, page13, Brightness, Contrast, Color Controls, page 13 and Appendix B, page 30)
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Antibiotic Reactions, Measurement of Inhibition Zones
To measure inhibition zones (see Illustration 46) of an
antibiotic reaction, click ‘ANTIBIOGRAM’ in the Tools
menu or click ’ANTIBIOTIC’ in the Control Panel. The
control panel will display controls suitable to antibiotic
use (see Illustration 49). To get back to colony counting,
click on ’COLONY’ in the Control Panel or deselect
‘ANTIBIOGRAM’ in the Tools Menu.
Illustration 46: Click Antibiotic to
get the Antibiotic Controls Up
Illustration 47: Measuring Inhibition Zones
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Finding Inhibition Zones
To find zones of inhibition, click on the ’FIND’ button in the control panel.
Illustration 48: Click the Find Button to
Measure Inhibition Zones
This will locate all antibiotic discs and will simultaneously determine the diameter
of inhibition zones. Measurements are calculated assuming an 86mm (inner
diameter of a 90mm plate) total width. The diameter of the disc is included in this
measurement.
If, in some cases where visibility is poor, due to agar color or unusual growth
patterns, the program may fail to find the correct measurement. In these cases, a
manual adjustment to the measurement is recommended. This can be done by
right-clicking and dragging the mouse, releasing the mouse once you have reached
the outside of the inhibition zone. A blue circle (see Illustration 49) will appear
once you start dragging the mouse, indicating the new size of the inhibition zone,
should you release the mouse button.
Illustration 49: Resizing Inhibition Zones
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View
List View and Image View
From the View menu the following options are available:
Outline CFUs
Advanced View
Live Video Feed
List View
Image View.
Illustration 50: View Menu
Outline CFUs
Check or uncheck this option to outline the parameter of colonies or highlight the
entire colony.
List View
When List View is selected from the View menu, the Image Chooser panel displays
only small icons and the image name.
Illustration 51: Small Icons are Displayed in List View
Image View
When Image View is selected from the View menu, large icons and the count are
shown in the Image Chooser Window.
Note: Image View selection is the default selection.
Illustration 52: Larger Images are Displayed in Image View
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Help
The help menu has three items:
About - will give company name and model information
User Guide - will open up this document.
Contact information - for Revolutionary Science's Technical support.
Email: support@revsci.com
Telephone contact: 651-674-3112
www.revsci.com
Illustration 53: Help Menu
Appendix A – Color Space
The IncuCount uses the standard red, blue, and green (sRGB) color space. Colors
can be broken down into 3 separate components of: Red, Blue, and Green. Each
component ranges from 0 to 255. “Pure” red would be represented as a nonzero
red component, blue and green components both would be zero. Darker colors
will be represented by values closer to zero, while brighter colors will be
represented by values closer to 255. For example, bright purple might be
represented by 200 in the red component, 190 in the blue component, and 0 in the
green component.
An important note to how the sRGB color space is used in the IncuCount
software is that only two components will be non-zero when a color is selected
for agar or colony type. This is to subtract brightness to make it easier to compare different colors.
For a more in depth discussion on the sRGB color space, refer to the sRGB entry
in Wikipedia.
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Appendix B–Camera Settings
Exposure
Exposure is akin to the iso speed of a 35mm camera. The longer the Exposure,
the less lighting the camera needs to develop a picture but the picture will be
more grainy.
Gain
Gain boosts the visual signal coming into the camera.
Brightness
Adjusting Brightness lightens or darkens the image.
Contrast
Adjusting contrast heightens or lessens the difference between light and dark areas
in the image.
Color Intensity
A low color intensity will look like a grayscale image. Colors are more pronounced with a higher color intensity level.
White Balance
Usually the camera will find the white balance for you. The camera attempts to
adjust colors based on what it thinks it white. You can change this using the
White Balance slider.
Anti-Flicker
Voltage from an AC power supply oscillates at 50 or 60 Hertz. Turning on this
feature attempts to account for that.
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