Gemini Automatic Stainer Operator Guide

Shandon Varistain
Gemini
®
AUTOMATIC STAINER
ENGLISH
Issue 4
OPERATOR
GUIDE
A78010402
A78010402 Issue 4
SHANDON VARISTAIN GEMINI
OPERATOR GUIDE
SYMBOLS
The following symbols and conventions are used throughout this manual and on the
instrument.
THIS SYMBOL WARNS YOU THAT YOU MUST FOLLOW INSTRUCTIONS FOR
SAFE AND CORRECT OPERATION. IF THIS SYMBOL APPEARS ON THE
INSTRUMENT, ALWAYS REFER TO THIS OPERATOR GUIDE.
THIS SYMBOL WARNS YOU THAT SURFACES ARE HOT. IF THIS SYMBOL
APPEARS ON THE INSTRUMENT, ALWAYS REFER TO THIS OPERATOR GUIDE.
THIS SYMBOL WARNS YOU THAT THERE MIGHT BE A BIOHAZARD
ASSOCIATED WITH THE INSTRUMENT. ALWAYS ACT WITH COMMON SENSE
AND BE AWARE OF THE SAMPLES USED. TAKE SUITABLE PRECAUTIONS.
THIS SYMBOL WARNS YOU THAT HARMFUL CHEMICALS ARE USED WITH
THE INSTRUMENT. REFER TO THE MATERIAL SAFETY DATA SHEETS FOR THE
CHEMICALS USED. ALWAYS ACT WITH COMMON SENSE AND BE AWARE OF
LOCAL LABORATORY PROCEDURES. TAKE SUITABLE PRECAUTIONS.
WARNING
A warning is given in the document if there is a danger of personal injury or
damage to samples or equipment.
Note
1 Notes give more information about a job or instruction but do not form part of the
instruction
All information contained in this manual is proprietary and confidential, and the exclusive property
of Thermo Electron Corporation. This manual is protected by copyright and reproduction is
prohibited. This manual is for use only by the individuals to whom it has been made available by
Thermo Electron Corporation.
1
2
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
SAFETY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.1
OVERVIEW . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
INSTALLATION AND SETTING UP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1
2.2
2.3
2.4
2.5
2.6
2.7
2.8
2.9
2.10
PROCEDURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
TO UNPACK . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
TO LEVEL THE INSTRUMENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
TO FIT THE FILTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
TO CONNECT THE WATER SUPPLY AND DRAINAGE . . . . . . . . . . . . . . . .14
TO ADJUST THE TURNTABLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
TO CONNECT THE SYSTEM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
ELECTRICAL REQUIREMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
TO SWITCH ON AND OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
CONTROLS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
3.1
3.2
3.3
DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
MAIN CONTROL PANEL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
LOAD/UNLOAD CONTROLS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
4.1
4.2
4.3
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
HOW TO STAIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
HOUSEKEEPING TASKS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
CLEANING AND MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
5.1
5.2
CLEANING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63
MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
TROUBLE SHOOTING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
®
C
US
Thermo Electron Corporation makes every endeavour to ensure that the information contained in its support documentation is
correct and clearly stated but does not accept responsibility for any errors or omissions. The development of Thermo products and
services is continuous. Make sure that any published information that you use for reference is up to date and relates to the status
of the product. If necessary, check with Thermo or your local Thermo representative.
© 2003 Thermo Electron Corporation. All rights reserved.
This manual may not, in whole or in part, be copied, photocopied, reproduced, translated, or converted to any
electronic or machine readable form without prior written consent of Thermo.
2
WELCOME . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company
Thermo Electron Corporation is the trading name of Thermo Shandon Limited
The Shandon Varistain Gemini meets the following CE Mark
requirements:
In Vitro Diagnostic Directive 98/79/EC
Low Voltage Directive 73/23/EEC, as amended by 93/68/EEC.
LIST OF CONTENTS
A78010402 Issue 4
6.1
TABLE
TABLE
TABLE
TABLE
TABLE
TABLE
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65
1 - EQUIPMENT STATUS CHECKS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66
2 - NUCLEAR STAINING PROBLEMS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
3 - CYTOPLASMIC STAINING PROBLEMS . . . . . . . . . . . . . . . . . . . . . . . . .71
4 - MISCELLANEOUS STAINING PROBLEMS . . . . . . . . . . . . . . . . . . . . . .75
5 - TROUBLESHOOTING HAEMATOXYLIN STAINING . . . . . . . . . . . . . . . . .78
6 - TROUBLESHOOTING PAPANICOLAOU STAINING . . . . . . . . . . . . . . . . .79
SPECIFICATION AND ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
7.1
7.2
SPECIFICATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82
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3
WARRANTY STATEMENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
APPENDIX A TRANSPORTATION INSTRUCTIONS . . . . . . . . . . . . . . . . . . . . 86
A.1
A.2
A.3
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
TO REPACKAGE THE STAINER UNIT . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
TO REPACKAGE THE POWER SUPPLY UNIT . . . . . . . . . . . . . . . . . . . . . . .90
APPENDIX B APPROVED REAGENT LIST . . . . . . . . . . . . . . . . . . . . . . . . . . 91
B.1
B.2
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
REAGENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
APPENDIX C EXAMPLE STAINING PROCEDURES . . . . . . . . . . . . . . . . . . . 92
C.1
C.2
C.3
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92
HAEMATOXYLIN AND EOSIN STAINING ROUTINE . . . . . . . . . . . . . . . . . .92
PAPANICOLAOU (PAP) STAINING ROUTINE . . . . . . . . . . . . . . . . . . . . . . . .93
APPENDIX D TO PERFORM A MEMORY RESET . . . . . . . . . . . . . . . . . . . . . 95
D.1
D.2
WELCOME
1
INTRODUCTION
Welcome to the Shandon Varistain Gemini stainer. Designed and made with care, the
instrument is safe to use, simple to operate, and easy to maintain.
This Operator Guide gives instructions for the correct operation and use of both
versions of the Shandon Varistain Gemini stainer.
2
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
INSTRUCTIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
SAFETY
THIS PARAGRAPH DETAILS IMPORTANT SAFETY INFORMATION. PLEASE
READ THIS PARAGRAPH CAREFULLY.
Thermo products are designed for convenient and reliable service. However, incorrect
actions by a user may damage the equipment, or cause a hazard to health. It is
important for you to know that:
i
The Shandon Varistain Gemini, as supplied, conforms with IEC1010-1.
However, the addition of chemicals introduces potential hazards.
As with all scientific equipment, due care and good laboratory practice
must be employed when dealing with these chemicals, and consideration
must be given to the potential for hazard when dealing with particular
chemicals.
Be aware that many of the reagents used with the Shandon Varistain Gemini
are flammable. Do not introduce any source of ignition into, or near, the
instrument once it has been loaded with reagents.
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A78010402 Issue 4
ii
The Shandon Varistain Gemini stainer weighs approximately 80 kilograms
(176lbs); get help to move or lift it.
iii
Do not remove any panels or covers. The Shandon Varistain Gemini stainer
does not have any user serviceable parts inside the instrument or power
supply unit.
A78010402 Issue 4
5
iv
This instrument must be properly connected to a good earth (Ground) via
the mains input supply.
Notes
1 If the Shandon Varistain Gemini has mainly been operated in very low
temperatures, or has been exposed to frequent mains failures, the battery
should be replaced every year.
In compliance with statutory requirements all our equipment is designed to
accepted standards of safety. Its use does not entail any hazard if operated
in accordance with the instructions given in this manual. However, you
must obey the following safety precautions:
2
The battery manufacturer (Yuasa Battery Sale (UK) Ltd) advise their customers
to comply with the relevant regulations within their particular country regarding
disposal of this type of battery.
3
The battery used within the Shandon Varistain Gemini Power Supply Unit is :NP12-12, 12V, 12Ah, valve regulated sealed lead-acid type rechargeable battery
i
All users must read and understand the Operator Guide, and only operate the
unit in accordance with the instructions.
ii
Potentially lethal voltages above 110V a.c. or 50V d.c. are present inside
the Power Supply Unit (PSU). Do not remove any access covers unless
specifically instructed to do so.
This battery is classified as, “Class 8, Group III UN No. 2800 Batteries, wet, nonspillable, electric storage, special provision A67”, and meets all requirements
of the International Air Transport Association, (I.A.T.A. Dangerous Goods
Regulations).
ix
Safety and Chemicals
The Shandon Varistain Gemini, as supplied, complies with IEC1010-2-010.
iii It is important that normal standards of safety and good laboratory practices
are employed. Always use common sense when operating the instrument.
However, chemicals introduce potential hazards and Thermo has adopted
the following position with regard to the subject of heated surfaces and
volatile chemicals used in medical laboratories:
iv Any problems and queries should be referred to your Thermo supplier.
v
Correct maintenance procedures are essential for consistent performance. It
is recommended that a Maintenance Contract is taken out with our Service
Department.
vi Use only factory approved accessories or replacement parts with the
stainer.
i
Non-specified chemicals are used in the instrument at the customer’s own
risk.
ii
All the chemicals recommended by Thermo have auto-ignition temperatures
considerably above any surface temperature that can be reached during a
single fault failure of the heating system on the instrument. Small quantities of
paraffin wax present on slides will not reach a temperature that will produce
flammable vapour.
vii Only use reagents recommended in Appendix B.
viii Disposal of Sealed Lead Acid Battery used in Power Supply Unit (PSU):
The Sealed Lead Acid battery within the Shandon Varistain Gemini Power
Supply Unit will need to be replaced every three years.
6
A78010402 Issue 4
iii The instrument contains no source of ignition in any areas of the instrument
where chemicals are stored or likely to leak in a single fault condition.
A78010402 Issue 4
7
iv The operator is fully aware of the contents of the specification documents
detailing the properties of the chemicals they are using.
DESCRIPTION
v
1.1 OVERVIEW
The operator has carried out any legally required assessment of chemicals
used and is using good laboratory practice.
Attention should be given to the flammability of chemicals used
WARNING during staining, and sources of ignition should be kept away from
d
a
the instrument once chemicals have been added.
The Shandon Varistain Gemini stainer has
been designed for general laboratory use.
It can stain multiple batches of 20 slides at
any one time using different procedures.
It uses two levels for reagent troughs
and other facilities - giving a maximum
of 26 reagent troughs, six running water
positions, and five dry storage or optional
heater positions.
f
e
The Shandon Varistain Gemini stainer is
a versatile precision engineered benchtop slide stainer, capable of staining large
numbers of microscope slides easily and
efficiently.
l
c
b
k
j
i
h
g
Figure 1.1
The different components of the Shandon Varistain Gemini stainer are shown in Figure
1.1.
a
b
c
d
e
f
g
h
i
j
k
l
8
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Monitor
Reagent troughs (on both layers)
Load / Unload touch panel
Water wash positions
Air extraction outlet
Access door for loading slide baskets
Lower level turntable to aid reagent replenishment
31⁄2” floppy disk drive
Main doors for reagent replenishment, cleaning etc
Access door for unloading stained slides
Main control panel
Power Supply Unit (PSU)
A78010402 Issue 4
9
INSTALLATION AND SETTING UP
A maximum of 26 reagent troughs, 6 running water positions, and 5 dry holding
or optional heater positions.
ii
Multiple baskets of 20 slides can be processed at the same time
iii
Loading and unloading of slide baskets is an easy, one-handed operation
iv
Batches of slides are easily transferred to the Shandon Consul automatic
coverslipper by the use of the Shandon Consul Clip Transfer Tool
v
Heating stations (up to 5) for slide drying (heated units only)
vi
Optional agitation of slides
vii Running water wash with automatic flow regulation
viii Multiple procedures can be used at any one time
Ability to assign a run as urgent or routine, or to cancel a procedure and retrieve
slides
x
Visible and audible indication of completion of procedure
xi
Use with a standard PC compatible colour monitor
xii Battery back-up allows the instrument to be used for up to 40 minutes after mains
power failure to complete staining
xiii Parallel Centronics printer output is available to provide a record of runs and to
assist in reagent management
xiv 31⁄2” floppy disk drive allows for easy software update and data exchange
Routine maintenance of the Shandon Varistain Gemini stainer requires only that the
charcoal filter is regularly changed and that the instrument is kept clean.
The Shandon Varistain Gemini stainer is a bench mounted instrument that must be
unpacked and installed with care.
The Shandon Varistain Gemini stainer must be positioned within 1.8m (6 ft) of a drain
and water supply.
The bench should be rigid and level, and
made of a non-flammable material. It must
be at least 600mm (24ins) deep and
be large enough for the Power Supply
Unit and Monitor to be sited near to
the Stainer. It must be able to hold their
combined weight (Stainer: 80kg (176lbs;
Power Supply Unit: 11kg (24lbs); Monitor:
20kg (44lbs)).
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ix
2.1 PROCEDURE
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Special design features of the Shandon Varistain Gemini stainer include:
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<1.8m
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Make sure that there is at least 100mm
(4”) clearance around the fan inlet on the
left hand side of the instrument.
The maximum overall dimensions of the
Shandon Varistain Gemini stainer are:
Width
720mm
(281⁄2ins)
Depth
710mm
(28ins)
Height
880mm
(341⁄2ins)
Weight
80kg
(176lbs)
>100mm
>100mm
The Stainer weighs approximately 80 kilograms (176lbs). Get help to
WARNING safely move or lift the instrument without risk of injury.
10
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11
The Stainer weighs approximately 80 kilograms (176lbs). Get help to safely
The maximum overall dimensions of the associated Power Supply Unit are:
Width
360mm
(14ins)
Depth
463mm
(181⁄4ins)
Height
145mm
(61⁄2ins)
Weight
11kg
(24lbs)
WARNING move or lift the instrument without risk of injury.
Remove the packaging that was used to secure the reagent troughs, baskets and arm
from inside the stainer unit.
Note
1 The large evaporation lids are packed inside the instrument
aa
Make sure that you have received all the parts listed on the packing list supplied with
the instrument. Contact your Thermo dealer if necessary.
2.2 TO UNPACK
If the packaging has been damaged, check the
condition of the instrument. Contact your Thermo dealer
if there is any damage.
Notes
1 Inform your Thermo dealer immediately if there are any breakages or shortages.
Quote the instrument Serial Number, your Order Number, Invoice Number,
Delivery Note (or Packing Slip) Number and the date.
b
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Remove the top layer of packaging from the Shandon
Varistain Gemini Power Supply Unit. Get help if necessary
to lift the instrument from the box and to place it on the
bench.
����
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2
For optimum performance of a heated Shandon Varistain Gemini, make sure that
there is at least 100mm (4”) clearance around the fan inlet on the left hand side
of the instrument.
3
If you ever need to transpor t the instrument, refer to Appendix A for
instructions about how to repack the instrument.
�
To lift or move the Shandon Varistain Gemini Power Supply
Unit, hold the box securely under the sides (a) of the unit.
2.3 TO LEVEL THE
INSTRUMENT
Get help if needed to remove the monitor
(maximum allowable monitor weight 30kg
(66lbs)) from its packing and place on top
of the Power Supply Unit (b) (follow the
manufacturer’s instructions).
The Shandon Varistain Gemini stainer must
be level before it is used for the first time. It
is fitted with levelling feet that may need to
be adjusted to suit its working location.
Remove the top layer of packaging from the
Shandon Varistain Gemini stainer. Get help
to lift the instrument from the box and to
place it on the bench.
To adjust the levelling feet, first use a
spanner to loosen the lock nut (a).
c
c
To lift or move the Shandon Varistain Gemini
stainer, hold the instrument securely under
the sides (c).
Adjust the height of the foot with a spanner
on the lower nut (b). When the height is
correct, tighten the lock nut (a).
a
b
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2.4 TO FIT THE FILTER
Water pressure must be between a minimum of 0.2bar (3psi) and a
WARNING maximum of 10bar (145psi). If the water pressure is greater than 10bar
A CARBON FILTER MUST BE INSTALLED IN THE SHANDON VARISTAIN GEMINI
STAINER AT ALL TIMES. IF NO FILTER IS FITTED, THE EXTRACTOR FAN
CANNOT BE OPERATED.
a
Note
1 An optional vent kit is available to
allow any fumes to be extracted
into a fume cupboard or vented to
the outside atmosphere.
(145psi), install a pressure reducing valve.
Note
1 A flow regulator is fitted to the
instrument to automatically regulate
the water flow.
2.5.2
To Connect the Drainage
The drain hose is already fitted to the
instrument (e). It should be run across the
back of the instrument to the drain point (f).
Remove the filter from its packaging.
Remove the cellophane wrapping and
place the filter into the front recess of the
top cover channel (a). Make sure that the
‘air flow’ arrow is visible from the front of
the unit and that it is pointing upwards into
the cover.
e
WARNING Make sure that the drain hose
f
is laid flat across the bench.
Notes
1 Shorten the drain hose as required to keep it as short as possible.
b
Slide the filter along the channel towards the rear of the unit. Make sure that it slides
over the spring flaps, and that it is secure against the rear of the channel (b).
2
To clean the inside of the drain hose, flush through with 10% commercial bleach.
2.5 TO CONNECT THE WATER SUPPLY AND DRAINAGE
2.5.1
2.6 TO ADJUST THE TURNTABLE
To Connect the Water Supply
The Turntable on the lower level of the
Shandon Varistain Gemini stainer can be
moved to facilitate cleaning and loading of
reagents. It must be in position and locked
before starting to stain.
A 2.5m (8 ft) supply hose is supplied with the
instrument. This must be fitted before use.
To fit the supply hose, screw the end with
the 90° elbow to the threaded fitting on the
back of the instrument at the left hand side
(c) (looking at the instrument from the rear).
Secure the other end to a water supply (d).
d
To adjust the Turntable, open the Main
Access Doors. Lift the two black Locking
Knobs (a) on the Turntable until they click
into the raised position.
a
c
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15
Gently rotate the Turntable to load reagent troughs or to clean the instrument. (Refer to
Section 4.2.8 for instructions about how to load reagents into the instrument).
Note
1 Do not turn the Turntable quickly if reagents are loaded in the instrument reagents are likely to spill.
To lock the Turntable, gently rotate the Turntable until positions C and D are in the
centre of the front of the instrument. Push the two Locking Knobs down and gently
move the Turntable a small distance from side to side until the Locking Knobs locate
into their locking positions.
Note
1 If the monitor’s data cable is too short to reach the stainer, use the extension
lead that is supplied with the Shandon Varistain Gemini stainer.
2.7.2
Connect the 25-way data cable to the middle
connector on the Stainer (a) and the 25-way
connector on the rear of the Power Supply Unit
marked ‘IOIOI’ (c).
Note
Check that the Locking Knobs are both locked in the lowered position.
1
Note
1 The Main Access Doors will not close if the Turntable is not in the correct
position, or if either Locking Knob is raised.
2.7.3
2.7 TO CONNECT THE SYSTEM
WARNING
2.7.1
The right hand connector (b) on the stainer
(i.e. the connector nearest to the front of
the instrument) is for service engineer use
only.
To Connect a Printer
Any dot matrix or inkjet printer that typically
connects to the LPT1 Centronix printer port of an
IBM* compatible computer is suitable for use with
the Shandon Varistain Gemini stainer.
Make sure the power is switched off
before connecting any cables, otherwise
damage can occur.
To Connect the Monitor to the Stainer
The printer connects only to the Power Supply
Unit and produces a hard copy record of the
activity of any procedure on demand.
Consult the monitor’s instructions to identify the data
cable. Connect the monitor’s data cable to the left hand
connector on the Stainer (a) (i.e. the connector nearest
to the rear of the instrument marked with a monitor
symbol).
b
a
To connect a printer, use a properly screened and
terminated data input cable that does not exceed
3m in length. The cable connects from the right
hand connector on the rear of the Power Supply
Unit (d) (marked with a printer symbol) to the
printer.
Follow the manufacturer’s instructions to connect the
monitor’s power supply lead to the mains power supply.
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Product:
Cat No:
Serial No:
Nominal Ratings:
* IBM is a registered mark of International
Business Machines.
a
16
To Connect the Stainer to the Power Supply Unit
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A78010402 Issue 4
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PS0001E0001
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17
2.8 ELECTRICAL REQUIREMENTS
THE STAINER IS LOW VOLTAGE (40V D.C.). IT MUST ALWAYS BE POWERED BY
THE SHANDON VARISTAIN GEMINI POWER SUPPLY UNIT.
Make sure that the voltage of the mains
supply corresponds with the voltage rating
on the rating plate on the back of the Power
Supply Unit.
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Product:
Cat No:
Serial No:
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A78010351 Issue 10
PS0001E0001
Nominal Ratings:
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Once the Power Supply Unit has been
connected to both the Stainer and the mains
supply, press the I (ON) side of the Battery
Isolation Switch (d).
Brown wire
- Live (L or L2)
terminal
Blue wire
- Neutral (N or L1)
terminal
Green / yellow wire - Earth terminal E, ground or
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Notes
Note
1
�
During shipping the Battery Isolation Switch
(c) will be switched to the OFF position, (O
on Battery Isolation Switch pushed inward).
a
The ~ symbol on the rating plate indicates that
the instrument operates on an alternating current
supply (a.c.)
Make sure that the I / O power switch at the rear of
the instrument is switched OFF (O side of the switch
pushed inward) (a).
Instruments are supplied with power cords with
moulded plugs suitable for the UK, France and Germany or North America. If another
plug is required, it is necessary for a technically competent person to remove the
moulded plug and fit a suitably rated, fused plug using the wiring convention shown
opposite.
The Battery Isolation Switch should
be left in the ON position until
the 12V battery is checked at the
annual service, or if the PSU is to
be transported.
2
Make sure that the correct
sequence is followed when the
instrument is switched off (see
Appendix A).
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A78010351 Issue 10
PS0001E0001
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If the correct sequence is not
carried out, the instrument may
not operate correctly when it is
switched on again. If this happens,
follow the instructions in Appendix
D to perform a memory reset.
l
0
Insert the appropriate mains cable into the mains connector on the rear panel of the
Power Supply Unit (b) and clip the cable restraint over the mains connector. Connect
the mains supply cable to the local power supply outlet.
1
2.9 TO SWITCH ON AND OFF
18
A78010402 Issue 4
A78010402 Issue 4
d
l
IT MUST BE POSSIBLE TO INTERRUPT THE MAINS SUPPLY AT SOURCE BY
REMOVING THE PLUG FROM THE MAINS SUPPLY SOCKET.
THE POWER SWITCH ON THE
MAINS INLET SOCKET SHOULD
ALWAYS BE USED TO TURN THE
POWER SUPPLY UNIT ON AND
OFF.
0
THE SHANDON VARISTAIN GEMINI STAINER POWER SUPPLY MUST
BE PROTECTIVELY EARTHED. MAKE SURE THAT IT IS PLUGGED INTO A
PROPERLY EARTHED MAINS SUPPLY.
19
IF THE BATTERY ISOLATION
SWITCH IS USED TO SWITCH
OFF THE INSTRUMENT, ALL
OPERATIONAL
STATUS
INFORMATION WILL BE LOST.
If the instrument is to be left unattended for long periods of time, or is to be moved,
the power to the instrument should be turned off. Press the O (OFF) side of the power
switch to switch off the Shandon Varistain Gemini (e).
a
Varistain Gemini
Welcome
ALL BATCHES IN PROGRESS
MUST BE REMOVED FROM THE
INSTRUMENT BEFORE THE
INSTRUMENT IS SWITCHED ON
AGAIN.
1/1/2000
THE SHANDON VARISTAIN GEMINI HAS A BATTERY BACK UP IN CASE OF
POWER FAILURE. BE AWARE THAT THE ARM MAY MOVE EVEN IF THE MAINS
SWITCH IS SWITCHED OFF.
10:42
WELCOME TO VARISTAIN GEMINI STAINER
2.10
Press 1 for English
Appuyer 2 sur Francais
Drucken sie 3 fur Deutsch
ACCESSORIES
2.10.1 Shandon Consul Clip Transfer Tool
Please press SELECT once you have entered the required language
2.9.1
To Switch On
If help is required at any point press the i button
Use the EXIT key to return from most screens to the previous menu
Press the I (ON) side of the I / O power
switch to switch the instrument on (a).
Initial Battery Charging - after connecting
the Power Supply Unit to the Stainer and
switching on, it should be left for a period
of 14 hours (overnight), to ensure the
battery is fully charged.
Select
d
b
To load the Shandon Consul Clip Transfer Tool,
place the Transfer Tool on a flat surface facing
upwards and with the handles fully extended (a).
c
Insert a 20 Clip Rod into the Transfer Tool with the
open end of the Slide Clips facing upwards (b).
The Rod locates into the cut out in the end of the
Transfer Tool (c).
c
b
h
Note:
1 The rod will only fit in the Tool this way
round.
d
Follow the instructions on the screen to
select the required language.
For example, press 1 for English (b)
and then press the left hand function
button (c) on the Main Control Panel that
corresponds to [SELECT].
a
e
Push the 20 Clip Rod down firmly into the
Transfer Tool (d).
g
Squeeze the handles together (e) and invert
the Transfer Tool over the basket of Microscope
Slides (f).
f
e
Line up the Slides with the Clips for correct orientation into the Consul Coverslipper.
2.9.2
To Switch Off
When the instrument is regularly used, the Standby button [
] (d) on the Main
Control Panel should be used (see Section 3.2.10).
20
Note:
1 Make sure the sample side of the Slides (g) face away from the protruding rod.
Keep the handles squeezed together. Push the Transfer Tool down (h) until the Slides
are fully located in the clips.
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21
Release and fully open the handles of the Transfer
Tool (i).
i
2.10.2 Vent Kit
j
i
Make sure that no pressure is applied to the
handles when the Transfer Tool is removed from the
slides (j).
p
m
o
n
b
c
Fix the mounting bracket to the top cover over
the fan outlet grille (c). Use the 4 screws and
gasket provided.
l
k
Remove the filter if fitted from inside the top cover.
To fit the fan guard provided (d) on the inlet side of the extraction fan inside the
instrument, locate the 4 plastic lugs on the fan guard (e) in the 4 corner holes of the fan
and push firmly into place.
Push the first Adaptor Collar (o) over the Rod (with the
small diameter first) until it is tight against the clips. Use the
Allen Key provided (1.5mm A/F) to tighten the grub screws
(q) to secure the collar.
q
a
The flexible duct (a) should be fixed to the mounting
bracket using the large clip provided (b).
The 20 Clip Rod can now be placed on the Consul
for coverslipping.
Note:
1 The samples on the slides should face away from
the Adaptor Collars.
The Vent Kit should not be used to extract the fumes through the
building HVAC system or a common site extraction system.
The optional vent kit allows any fumes to be
extracted into a fume cupboard or hood, or
vented to the outside atmosphere. Maximum
airflow is 58m3 per hour.
Hold the 20 Clip Rod (k) and pull it upwards to
remove the slides from the Basket (l).
To fit the 20 clip adaptor collar, fit 20 Slide Clips (m) on the
standard 40 Clip Rod (n). Make sure the clips are oriented
in the correct way.
WARNING
Use the rectangular block to activate
the filter sensor. Fit the block in the left
hand filter channel, on the underside of
the top cover (f).
Fit the second collar (p) at the end of
the Rod so that the end of the collar sits
between the two grooves. Use the Allen
Key provided (1.5mm A/F) to tighten the
grub screws (q) to secure the collar.
To remove the 20 clip adaptor collar, loosen
the grub screws (q), and slide both collars
off the rod.
f
e
d
22
A78010402 Issue 4
A78010402 Issue 4
23
CONTROLS
Orientate the block so that if fits in the
channel and is retained by the filter location
spring. Push the block firmly to the back of the
channel to activate the sensor (g).
3.1 DESCRIPTION
g
The filter life warning in the user interface
must be switched off. The filter life set-up
option is within the Quality Control menu.
Change the Filter Replacement Warning
Interval to ‘Off’.
The Shandon Varistain Gemini stainer is a versatile precision engineered automatic
stainer. A separate Power Supply Unit (PSU) and monitor are sited beside the stainer
on the bench.
The Shandon Varistain Gemini stainer has been designed for general laboratory use. It
can process multiple batches of 20 slides using different procedures.
Note
This chapter describes the functions of all the controls on the instrument.
The flexible duct can be cut to length as required.
2.10.3 Large Slide Basket Adaptor
The Main Control Panel is situated on the
Power Supply Unit (PSU).
a
3.2.1
The function of each of the four blank
buttons at the top of the Main Control Panel
(a) corresponds to the specific function of
the buttons shown at the bottom of each
display screen on the colour monitor.
If required, the adaptors can be fitted in
different positions to allow multiple sized
slides to be used
a
A78010402 Issue 4
A78010402 Issue 4
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24
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Slide the adaptors into the slots in the basket as
shown (a), and insert up to four 75 x 58mm (3” x
21⁄4”) slides in the slots in the adaptor.
Hint
1
Function Buttons
�
The Large Slide Basket Adaptor has been
designed to allow large slides to be used in the
Shandon Varistain Gemini slide baskets (up to 75
x 58mm (3” x 21⁄4”)).
3.2 MAIN CONTROL PANEL
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1
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a
25
An example is shown here:
Main Menu
To choose [STAIN], press the left hand
function button (a) as it corresponds to the
one shown on the display. The instruction
given to press this function button will be
“Press [STAIN]”.
Press function button (b) to choose
[PROGRAM]. The instruction given to
press this function button will be “Press
[PROGRAM]”.
3.2.4
Varistain Gemini
1/1/2000
Cancel
10:42
Press [CANCEL] to cancel any selection.
Please select the required action
Press STAIN for information on using the Varistain Gemini to stain slides
Press PROGRAM to define or view reagents, procedures and reagent layout
Press MONITORING to view reagent usage and batch progress
Press OPTIONS to set up the Varistain Gemini
3.2.5
Direction Arrow Keys
If help is required at any time press the i button
Use the EXIT key to return from most screens to the previous menu
Press [
], [
the screen.
Stain
Program
Monitoring
b
c
] and [
] to move the cursor around
Options
3.2.6
a
], [
Exit
d
Press [EXIT] to go back to the previous screen.
Press function button (c) to choose
[MONITORING]. The instruction given to
press this function button will be “Press
[MONITORING]”.
3.2.7
Press function button (d) to choose [OPTIONS]. The instruction given to press this
function button will be “Press [OPTIONS]”.
[
]
This key is currently not used, but is reserved for future
features.
3.2.8
Silence Alarm [
]
Note
1
This convention for pressing the function keys will be used throughout this
Operator Guide.
Press [
] to silence the alarm. For some alarms, this will
cancel the alarm as well. Other alarms will require separate
actions and acknowledgements.
3.2.2
Number Keys
3.2.9
Help Key [
]
The number keys are used to input numerical
information for use in staining procedures and to
select different programs.
Press [
3.2.3
3.2.10 Standby Key [
Enter
Press [ENTER] if required to confirm any selection.
Press [
] to display a help screen at any time.
]
] to switch the instrument into standby mode. The
red LED flashes when Standby is selected. If [
again, the instrument will restart.
26
A78010402 Issue 4
A78010402 Issue 4
] is pressed
27
The Load / Unload Touch Panel is located on
the front of the Stainer.
4.1 INTRODUCTION
3.3.1
It is recommended that the instrument is
Load Buttons [A] and [B]
kept in Standby mode when it is not in use.
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To enter the Standby mode, press the [
����
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�
]
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button (a).
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These buttons can be used to start the
staining of a batch of slides once they have
been loaded.
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3.3 LOAD/UNLOAD CONTROLS
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a
Note
1
If the LED is lit, the relevant load door is
available for a basket of slides to be loaded.
A flashing LED on Load Buttons [ A ] and
[ B ] indicates that a load door has been
opened, but has not been acknowledged.
Press the button to confirm that slides have
been loaded.
The red LED flashes when the
instrument is in STANDBY mode (b)
To restart the instrument, press the [
b
] button again.
4.2 HOW TO STAIN
Follow these simple steps to prepare the Shandon Varistain Gemini stainer for staining
slides:
If the LED is off, the load door is not available.
1
3.3.2
Unload Buttons [C] and [D]
2
The LED on each Unload Button flashes when the basket is in position to be unloaded.
Press Unload Buttons [C] and [D] to acknowledge the removal of a batch of slides after
staining.
3
4
If the LED is off, there are no baskets to be unloaded. Do not open the load door if the
LED is off.
3.3.3
Auto Return
Press [AUTO RETURN] to request the return of the last batch of slides loaded. The
slides will be returned to the input door where they were loaded to allow the operator to
remove them.
28
A78010402 Issue 4
Select the STAIN option from the
Main Menu (paragraph 4.2.1).
Define any special reagents to be
used (paragraph 4.2.2).
Define the staining procedures to
be used (paragraph 4.2.3).
Use the ‘Reagent Layout’ facility
to assign the most efficient use of
reagents (paragraph 4.2.5).
5
Load the reagents in their optimal
positions (paragraph 4.2.8).
6
Stain the slides (paragraph 4.2.9).
A78010402 Issue 4
Varistain Gemini
1/1/2000
Main Menu
10:42
Please select the required action
Press STAIN for information on using the Varistain Gemini to stain slides
Press PROGRAM to define or view reagents, procedures and reagent layout
Press MONITORING to view reagent usage and batch progress
Press OPTIONS to set up the Varistain Gemini
If help is required at any time press the i button
Use the EXIT key to return from most screens to the previous menu
Stain
Monitoring
Program
b
Options
a
29
Notes
Notes
1
To access help screens at any time, press [
2
To exit from most screens, press [EXIT] (b) to return to the previous screen.
4.2.1
] (a) on the Main Control Panel.
Main Menu
Press [STAIN] to enter the STAIN program
(i.e. press the left hand function button on
the Main Control Panel that corresponds to
[STAIN] on the monitor) .
The ‘Define Reagent’ screen lists 42
commonly used stains and reagents.
These cannot be changed, but a further
12 reagents can be added to the list if
necessary in the User Defined Reagents
section (reagent numbers 43 - 54 - shaded
in the illustration).
30
1/1/2000
10:42
Please select the required action
Press STAIN for information on using the Varistain Gemini to stain slides
Press PROGRAM to define or view reagents, procedures and reagent layout
Press MONITORING to view reagent usage and batch progress
Press OPTIONS to set up the Varistain Gemini
2
If any of the heater positions are to be used for the dry storage of baskets, then
it should be noted that ambient air is blown through the trough when any of the
other heaters are on.
3
The Heater Station troughs are
held in position by spring clips. To
remove them, pull upwards firmly.
To refit them, push them down
firmly so that the spring clip holds
the trough securely.
Varistain Gemini
How to Stain
1/1/2000
10:42
Quick Reference for using the Varistain Gemini stainer
1. Use DEFINE REAGENTS to enter the names of reagents to be used
2. Use DEFINE PROTOCOLS to enter the parameters for each program step
If help is required at any time press the i button
Use the EXIT key to return from most screens to the previous menu
3. Use REAGENT Layout to select the procedures currently required
4. Use LOAD REAGENTS to confirm each reagent has been placed in position
Stain
4.2.2 Define the Reagents to Be
Used
If a special reagent is used for a particular
procedure, it may be necessary to enter
the name of the reagent before any slides
can be stained.
The Heater Stations (reagent 41) are only fitted onto the heated version of the
Shandon Varistain Gemini stainer. They are intended to dry slides that are wet
with water only.
Varistain Gemini
Select the Stain Program
Once the required language has been
selected, the Main Menu will be displayed
on the monitor.
1
Program
Monitoring
Options
DO NOT SPLASH OR POUR
REAGENTS NEAR TO THE
HEATER STATION TROUGHS.
Varistain Gemini
Define Reagent Names
1/1/2000
Haematoxylin
Harris Unacidified
Harris Acidified
Gill 1
Gill 2
Gill 3
Aqueous Eosin
Alcoholic Eosin
Aqueous Eosin Y
Alcoholic Eosin Y
OG-6
EA-36
EA-50
EA-65
Xylene
Xylene Substitute
Alcohol
IMS
Ethanol
Isopropyl Alcohol
Buffer
Tap Water
Distilled Water
Deionised Water
Acid Alcohol
Acid Water
Bluing Reagent
Ammonia Water
Periodic Acid Soln
Schiffs Reagent
Sulphite Rinse
Van Gieson Stain
33
34
35
36
37
38
39
40
41
42
5. To stain a batch of slides open the Load Door and follow the on screen
instructions for selecting the procedure required
6. When a batch has completed its staining protocol it will be placed behind an
unload station door and its acknowledge button light will flash. Open the door
and follow the instructions on the screen.
Define Reagents
Define Protocols
Reagent Layout
Load Reagents
a
10:42
Use the cursor to select a name then press ENTER
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
DO NOT FILL HEATER STATION
TROUGHS WITH REAGENTS.
Alcian Blue
Perls Reagent
Leishman Stain
May Grunwald Stain
Giemsa Stain
Wright Stain
Jenner Stain
Dry Storage
Heater Station
Running Water Wash
User Defined Reagents
43
44
45
46
47
48
49
50
51
52
53
54
To enter a special reagent, press
[DEFINE REAGENTS] (a) from the ‘How
To Stain’ screen.
Use the direction arrow keys, [
] and
[
] to move the cursor to the next free
number.
When the correct line is highlighted, press
[ENTER] on the Main Control Panel. A
keyboard will be displayed on the screen.
Use the direction arrow keys to select each
letter of the new reagent name in turn.
Press [ENTER] when the correct letter has
been selected.
A78010402 Issue 4
A78010402 Issue 4
Varistain Gemini
Define Reagent Names
1/1/2000
10:42
Use the cursor to select a name then press ENTER
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
Haematoxylin
Harris Unacidified
Harris Acidified
Gill 1
Gill 2
Gill 3
Aqueous Eosin
Alcoholic Eosin
Aqueous Eosin Y
Alcoholic Eosin Y
OG-6
EA-36
EA-50
EA-65
Xylene
Xylene Substitute
Alcohol
IMS
Ethanol
Isopropyl Alcohol
Buffer
Tap Water
Distilled Water
Deionised Water
Acid Alcohol
Acid Water
Bluing Reagent
Ammonia Water
Periodic Acid Soln
Schiffs Reagent
Sulphite Rinse
Van Gieson Stain
33
34
35
36
37
38
39
40
41
42
Alcian Blue
Perls Reagent
Leishman Stain
May Grunwald Stain
Giemsa Stain
Wright Stain
Jenner Stain
Dry Storage
Heater Station
Running Water Wash
User Defined Reagents
43
44
45
46
47
48
49
50
51
52
53
54
31
When the name has been fully typed in,
press [COMPLETE] (b). This will return
you to the ‘Define Reagent Names’ screen.
2
Varistain Gemini
10:42
1/1/2000
Keyboard
To keep the procedure on the active
list, press [RETAIN]. This will allow
the times in the procedure to be
edited.
Varistain Gemini
1/1/2000
Procedure Select
10:42
The text going in
When all the required User Defined
Reagents have been entered, press [EXIT]
to return to the ‘How To Stain’ screen.
1
1
2
3
The User Defined Reagent names
can be up to 22 characters long.
/
.
,
M
N
B
3
]
‘
;
L
K
J
[
Use the cursor to select the procedure required and
then press SELECT
#
=
P
O
I
U
-
0
9
8
H
G
V
C
X
Z
\
Y
T
F
D
S
A
7
6
5
R
E
W
Q
Notes
4
3
2
4
Caps Lock
Control
Complete
To delete the last letter entered,
press [CANCEL] on the Main
Control Panel (c).
b
To switch between capital and lower
case letters, press [CAPS LOCK]
(d).
5
Varistain Gemini
10:42
1/1/2000
Keyboard
To remove the procedure from the
active list, press [REMOVE]. This
will remove the procedure from the
active list.
Once the procedure is removed
from the active list, it can be edited
as normal.
No.
Procedure Name
Notes
Duration
1
2
3
4
5
6
7
8
9
10
11
12
PAP
PAP
GYN
Non GYN
24:35
25:25
H&E
Routine
34:30
To reassign the procedure to
the active list, enter the Reagent
Layout 1 screen and reselect the
procedure (see paragraphs 4.2.5
and 4.2.6).
Varistain Gemini
The text going in
4
For other characters e.g. letters with
accents etc, press [CONTROL] (e).
(To exit from the Control Characters
Keyboard, press [CONTROL]
again).
4.2.3 Define
the
Procedures to Be Used
This step will
procedures.
define
Staining
the
staining
Press [DEFINE PROTOCOLS] in the ‘How
To Stain’ screen to enter the ‘Procedure
Select’ screen.
1/1/2000
Procedure Select
1
z
Caps Lock
d
x
c
y
t
b
v
n
m
<
{
>
}
@
:
l
k
j
h
#
=
p
o
i
-
0
9
8
u
g
f
d
s
a
7
6
5
r
e
w
q
|
4
3
2
?
There is space for 50 procedures. Use the
] to move
] or [
direction arrows [
to the required procedure, and press
[SELECT]. If the procedure chosen is an
existing one, the ‘Define Procedure’ screen
will be displayed.
If no procedures have been entered, or if
you want to enter a new procedure, select
an unused number. The ‘Select name’
screen will be displayed.
e
10:42
Use the cursor to select the procedure required and
then press SELECT
No.
Procedure Name
Notes
Duration
Changing the number of steps in a program that is currently
selected for use, or altering the reagent, might make the reagent
layout invalid.
Select RETAIN to keep this program in use or REMOVE to
remove the program from the reagent layout and allow the
program to be edited
10
11
12
Complete
Control
Change Notes
Change Name
Select
Retain
Remove
c
This screen is used to name a procedure from the seven pre-defined names or by a
user defined name. Use the direction arrows to select a name, or to choose an unused
number in the user-defined name section. Press [SELECT].
Note
1 A procedure can be viewed and the times can be edited while it is active. Steps
cannot be inserted or deleted, or the reagents changed. To fully edit an active
procedure, it must first be removed from the active list. If an active procedure is
selected, a warning box will be displayed.
32
A78010402 Issue 4
A78010402 Issue 4
33
If a user defined name was chosen, the
keyboard will appear.
Select Name
When the name has been fully typed in,
press [COMPLETE]. Another keyboard
will now be displayed to allow you to enter
or change the procedure notes.
2
10:42
Define Procedure Screen
This screen is used to specify the details
of each step to be used in a staining
procedure.
Varistain Gemini
Define Procedure
1/1/2000
Procedure no. 2 - H & E
The name of the procedure chosen and
any notes that have been entered are
displayed on the top line of the screen.
Select
Each procedure can have up to 50 steps.
They are organised into five colour coded
sections:
Step
Reagent
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
Dry Storage
Xylene
Xylene
Xylene
Alcohol
Alcohol
Alcohol
Running Water
Haematoxylin
Running Water
Acid Alcohol
Running Water
Aqueous Eosin
Running Water
Alcohol
Alcohol
Xylene
Xylene
Xylene
Insert Step
10:42
- Routine for Dr. xxxxxxx
Conc.%
100
100
100
100
100
Set
Uses.
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
Delete Step
Time /
Limit
Agitate
00:00
01:00
01:00
01:00
01:00
01:00
01:00
01:00
04:00
01:00
00:15
01:00
02:00
01:00
01:00
01:00
01:00
01:00
00:00
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
Standard
Standard
Standard
Standard
Standard
Standard
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
Copy
Change Reagent
Varistain Gemini
Keyboard
1/1/2000
10:42
Change Notes
The text going in
1
1 Load
Purple (1 step only)
2 Pre-Stain
Grey
3 Stain
Brown
4 Post-Stain
Green
5 Unload
Red (1 step only)
/
.
,
M
N
B
]
‘
;
L
K
J
#
=
[
P
O
I
U
-
0
9
8
H
G
V
C
X
Z
\
Y
T
F
D
S
7
6
5
R
E
W
Q
4
3
2
A
Notes
1 The User Defined Procedure
names can be up to 9 characters
long using capital and lower case
letters, ‘&’ and ‘.’ only.
1/1/2000
Select a name for the procedure from the list and
press SELECT or ENTER
Pre-defined names
User Defined Names
PAP
1
H&E
2
PAS
3
V. GIESON
4
GIEMSA
5
TRICHROME
6
ZN (TB)
7
8
9
10
11
12
13
14
Use the direction arrow keys to select
each letter of the new procedure name
in turn. Press [ENTER] when the correct
letter has been selected.
Use the direction arrows to choose each
letter in turn. Press [ENTER] when the
correct letter is selected. When the notes
have been entered, press [COMPLETE].
The ‘Define Procedure’ screen will now be
displayed.
4.2.4
Varistain Gemini
Notes
Caps Lock
Notes can be entered to identify any
special instructions or information
that may be helpful during the set
up or running of any procedure.
Control
Complete
Varistain Gemini
Keyboard
1/1/2000
1
[INSERT STEP] allows a step to be inserted below the cursor position
2
[DELETE STEP] deletes the selected step
3
[COPY] copies steps or sections from other procedures (or complete
procedures) for insertion into another procedure - for example standard steps
used for pre- or post-stain
10:42
The text going in
3
The procedure names and notes
can be altered at any time by
pressing
[CHANGE
NAME]
or [CHANGE NOTES] in the
‘Procedure Select’ screen.
!
¢
á
í
å
à
ó
ú
ñ
Ñ
¿
¡
Use the direction arrows [
²
34
] at any time to display
Press [
the help screen.
Caps Lock
Control
] to move the cursor
Hint
1
4
] and [
î
Ö
ÿ
ù
û
ò
~
+
ï
è
ë
ê
ç
_
)
(
*
ö
ô
É
Å
Ä
ì
&
^
%
ä
â
é
ü
Ü
$
£
“
To delete an entire procedure, use the copy facility to copy a blank procedure
over the one to be deleted.
Complete
A78010402 Issue 4
A78010402 Issue 4
35
Each step requires the following information (information marked * must be given):
b
a
a
Concentration
c
Set Uses
Varistain Gemini
* Time
e
Limit
f
Agitate
Define Procedure
1/1/2000
Procedure no. 2 - H & E
Note
1 Only reagent and concentration can
be entered in the Load and Unload
Sections
Step
Reagent
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
Dry Storage
Xylene
Xylene
Xylene
Alcohol
Alcohol
Alcohol
Running Water
Haematoxylin
Running Water
Acid Alcohol
Running Water
Aqueous Eosin
Running Water
Alcohol
Alcohol
Xylene
Xylene
Xylene
Insert Step
a
c
* Reagent
b
d
The number will be shown on the
Reagent Monitor Screen (accessed
through the [MONITORING] button
on the ‘Main Menu’ screen), and
an optional warning to alert the
operator can be selected in the
Options Screen (accessed from the
‘Main Menu’ screen).
10:42
- Routine for Dr. xxxxxxx
Conc.%
100
100
100
100
100
Set
Uses.
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
Delete Step
Time /
Limit
Agitate
00:00
01:00
01:00
01:00
01:00
01:00
01:00
01:00
04:00
01:00
00:15
01:00
02:00
01:00
01:00
01:00
01:00
01:00
00:00
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
Standard
Standard
Standard
Standard
Standard
Standard
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
Copy
If not required, do not enter anything
into this field.
d
Use the number keys to enter the
time required for the slides to be
immersed in that reagent (minutes:
seconds) (maximum 59 minutes
and 59 seconds).
Change Reagent
Reagent
Press [CHANGE REAGENT]. A list of the available reagents is displayed. Use the
direction arrows, or use the number keys to type in the reagent number, to select
the required reagent, then press [ENTER].
Note
1 The user defined reagent name can be changed or added at this point.
Use the direction arrows [
] and [
] to move the cursor to select a user
Time (minutes:seconds)
d
e
Varistain Gemini
1/1/2000
Define Procedure
Conc.%
Step
Reagent
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
Dry Storage
Xylene
Xylene
Xylene
Alcohol
Alcohol
Alcohol
Running Water
Haematoxylin
Running Water
Acid Alcohol
Running Water
Aqueous Eosin
Running Water
Alcohol
Alcohol
Xylene
Xylene
Xylene
Insert Step
10:42
- Routine for Dr. xxxxxxx
Procedure no. 2 - H & E
100
100
100
100
100
Delete Step
Set
Uses.
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
Time /
Limit
Agitate
00:00
01:00
01:00
01:00
01:00
01:00
01:00
01:00
04:00
01:00
00:15
01:00
02:00
01:00
01:00
01:00
01:00
01:00
00:00
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
Standard
Standard
Standard
Standard
Standard
Standard
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
Copy
Change Reagent
Press [ENTER] or use the direction arrow keys to move the cursor.
Note
1 When Heater Stations are required, use 15 minutes for a trial batch and then
adjust the time as required to suit the laboratory conditions and process
requirements.
defined reagent (reagents 43 - 54). Press the right hand function button,
e
[CHANGE NAME], and follow the instructions in section 4.2.2.
b
These settings help to optimise the speed and throughput of slides. The right
hand function button switches through the following options:
Concentration (%)
Use the number keys to enter the concentration of the reagent to be used (1 100%). Use to differentiate between concentrations of alcohol for example. If not
required, do not enter anything into this field.
c
1 NO MAXIMUM - use this setting to identify reagent steps where slides can be
safely left for extended times. This setting allows urgent slides to be let through
when multi-procedures are used.
Set Uses
Use the number keys to enter the maximum number of times you wish to use the
reagent before it must be changed (maximum = 99).
36
Limit
A78010402 Issue 4
2 CRITICAL - use this setting to give exact programmed times. For example,
if the step is programmed for 30 seconds, the step will always be 30 seconds
long.
A78010402 Issue 4
37
3 STANDARD - tolerance steps may be extended by up to 30% of the
programmed time. For example, a 30 second step may actually take between 30
and 39 seconds (i.e. 30 secs + 30%).
Press [EXIT] again to return to the ‘How to
Stain’ screen.
Varistain Gemini
1/1/2000
How to Stain
10:42
Quick Reference for using the Varistain Gemini stainer
Note
1 If a Heater Station is selected, ‘Limit’ is automatically set to ‘NO MAXIMIUM’.
This cannot be changed.
f
Agitate
f
Input the amount of agitation
required at each step. The right
hand function button switches
through the following options:
2 FREQUENT - agitation occurs
when the slides are first placed in
the reagent and then every minute
throughout the step
5. To stain a batch of slides open the Load Door and follow the on
screen instructions for selecting the procedure required
6. When a batch has completed its staining protocol it will be placed
behind an unload station door and its acknowledge button light will
flash. Open the door and follow the instructions on the screen.
Define Procedure
1/1/2000
Procedure no. 2 - H & E
Step
Reagent
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
Dry Storage
Xylene
Xylene
Xylene
Alcohol
Alcohol
Alcohol
Running Water
Haematoxylin
Running Water
Acid Alcohol
Running Water
Aqueous Eosin
Running Water
Alcohol
Alcohol
Xylene
Xylene
Xylene
Reagent Layout
10:42
- Routine for Dr. xxxxxxx
Conc.%
100
100
100
100
100
Set
Uses.
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
Time /
Limit
Agitate
00:00
01:00
01:00
01:00
01:00
01:00
01:00
01:00
04:00
01:00
00:15
01:00
02:00
01:00
01:00
01:00
01:00
01:00
00:00
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
Standard
Standard
Standard
Standard
Standard
Standard
No Maximum
No Maximum
No Maximum
No Maximum
No Maximum
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
Note
1 The Reagent Layout 1 screen is not accessible if batches are currently being
processed by the stainer. A warning screen will be displayed if Reagent Layout 1
is selected when the instrument is running
4.2.6
Reagent Layout 1 Screen
Varistain Gemini
How to Stain
Insert Step
Load Reagents
a
Press [REAGENT LAYOUT] (a) from the
‘How To Stain’ screen to enter the ‘Reagent
Layout 1’ screen.
Delete Step
Copy
Change Reagent
3 CONTINUOUS - agitation occurs all the time. Throughput is significantly
reduced if this option is selected
4 NONE - agitation is only used when the slides are first placed into the trough
Note
1 If a Heater Station is selected, ‘AGITATE’ is automatically set to ‘NONE’. This
cannot be changed.
When the procedure information is complete and correct, press [EXIT] to return to the
‘Procedure Select’ screen.
38
The Reagent Layout facility is used to
assign the reagents to the most efficient
layout to allow all selected procedures to
be run.
1. Use DEFINE REAGENTS to enter the names of reagents to be used
2. Use DEFINE PROTOCOLS to enter the parameters for each
program step
3. Use REAGENT Layout to select the procedures currently required
4. Use LOAD REAGENTS to confirm each reagent has been placed in
position
Define Reagents Define Protocols
Varistain Gemini
1 STANDARD - agitation occurs
when the slides are first placed in a
reagent, and then every 2 minutes
(approximately) apart from the last
minute of a step
4.2.5 Using the Reagent Layout
Facility
A78010402 Issue 4
This screen lists the procedures to be run
simultaneously on the Varistain Gemini
stainer.
Notes
1 [ADD PROCEDURE] introduces
a new procedure and adds any
reagents required into the free
troughs on the stainer
1/1/2000
10:42
Quick Reference for using the Varistain Gemini stainer
You cannot reprogram the instrument or alter its Reagent Layout
while it is staining slides. Wait for all batches to complete and to be
unloaded or use the Abort / Auto Return Facilities in the Monitoring
screens.
Press EXIT to return to the previous Menu.
6. When a batch has completed its staining protocol it will be placed behind
and unload station door and its acknowledge button light will flash. Open
the door and follow the instructions on the screen.
2
[DELETE PROCEDURE] removes
a procedure and allows the reagent troughs used by that procedure to be used
for other reagents
3
[REAGENT LAYOUT 2] is used to view the current reagent layout and allows the
operator to optimise the layout either manually or automatically
A78010402 Issue 4
39
4
If a procedure is chosen and the
required reagents will not fit into the
existing Reagent Layout, a warning
box will be displayed. If there are
duplicate reagent troughs that
can be removed so the procedure
will fit, then the program will give
the option to remove or retain the
duplicates
Varistain Gemini
Reagent Layout - 1
a
Batches per day (approximately)
b
Load Door (A & B) information
URGENT START - Use this to start an urgent procedure. It will ‘overtake’ any
standard procedures in use if it can do so without compromising the programmed
requirement of either procedure.
2
STANDARD START - Use this for routine procedures
3
DO NOT LOAD HERE - Use this selection (displayed as N/A) if you do not want
to start a procedure from this door.
10:42
Loading Door options : Select from Standard, Urgent or Not Used.
Procedure Name and notes Batches /day Door A Door B Door C Door D
H&E
PAP
PAS
Standard times
Dr X preferred times
25 Standard Standard No Print No Print
30 Urgent N/A
N/A
Print
2 Standard N/A
No Print N/A
The reagents for each Load Door and each Unload Door are currently as shown:
Load Door A
Unload Door C
Each procedure requires the following
information to be entered:
1/1/2000
1
:
:
Dry Storage
Load Door B
Xylene
Unload Door D
:
:
Dry Storage
Xylene
c
Unload door information
This option controls the operation of the unload acknowledge keys [C] and [D].
Add Procedure
Delete Procedure
Reagent Layout 2
The second function key (d) switches between the following options:
Varistain Gemini
Reagent Layout - 1
1/1/2000
10:42
Loading Door options : Select from Standard, Urgent or Not Used.
1
Procedure Name and notes Batches /day Door A Door B Door C Door D
c
Unload Door (C & D) information
Use the direction arrows to move across to
each field in turn.
The procedure selected requires additional reagents which are not
in the current Reagent Layout. There are insufficient duplicate pots
to allow this procedure on to run on the stainer without removing one
of the existing procedures. Press CANCEL and then use DELETE
PROCEDURE to create the number of unallocated reagent pots
required.
The reagents for each Load Door and each Unload Door are currently as shown:
Load Door A
Unload Door C
a
:
:
Dry Storage
Load Door B
Xylene
Unload Door D
:
:
Dry Storage
Xylene
UNLOAD - NO PRINT - Select this
option if you do not require a print
out
c
Varistain Gemini
Reagent Layout - 1
1/1/2000
10:42
Loading Door options : Select from Standard, Urgent or Not Used.
Procedure Name and notes Batches /day Door A Door B Door C Door D
H&E
PAP
PAS
Standard times
Dr X preferred times
25 Standard Standard No Print No Print
30 Urgent N/A
N/A
Print
2 Standard N/A
No Print N/A
Batches per day
Use the number keys to enter the
estimated number of times this procedure
will be run in one day.
a
Note
1 Print outs can be requested for
individual batches of slides from the
‘Acknowledge / Unload’ screen
b
Varistain Gemini
b
2
UNLOAD AND PRINT - Select this
option if you require an automatic
print-out of every batch as it is
unloaded
Load doors
1/1/2000
Reagent Layout - 1
3
10:42
Loading Door options : Select from Standard, Urgent or Not Used.
This option controls whether a procedure
can start from each load door, and the
priority that will be given to the procedure
if the load acknowledge button ([A] or [B])
on the Load/Unload Panel is pressed.
Procedure Name and notes Batches /day Door A Door B Door C Door D
H&E
PAP
PAS
Standard times
Dr X preferred times
25 Standard Standard No Print No Print
30 Urgent N/A
N/A
Print
2 Standard N/A
No Print N/A
The reagents for each Load Door and each Unload Door are currently as
shown:
Load Door A
Unload Door C
The second function key (d) switches
between the following options:
:
:
Dry Storage
Load Door B
Xylene
Unload Door D
Urgent Start
:
:
Dry Storage
Xylene
Reagent Layout 2
DO NOT UNLOAD HERE - Use this
selection (displayed as N/A) if you
do not want a procedure to finish at
this door.
The reagents for each Load Door and each Unload Door are currently as
shown:
Load Door A
Unload Door C
:
:
Dry Storage
Load Door B
Xylene
Unload Door D
Unload and Print
:
:
Dry Storage
Xylene
Reagent Layout 2
d
Notes
1 The cursor will not move to an invalid field - i.e. one where the reagent is
incorrect
2
The [ADD PROCEDURE] key will disappear if there are no more procedures that
can use the reagents currently in the load and unload positions
d
40
A78010402 Issue 4
A78010402 Issue 4
41
3
If a required procedure is not displayed on the ‘Reagent Layout 1’ selection list, it
could be that the procedure uses load or unload reagents not used by the other
listed procedures. To use the required procedure, either:
i
Select each of the other procedures in turn and press [DELETE PROCEDURE]
to remove them, or
ii
Select each of the other procedures in turn and switch the selection for one
load door (either [A] or [B]) and one unload door (either [C] or [D]) to N/A. (i.e.
select ‘DO NOT LOAD HERE’ and ‘DO NOT UNLOAD HERE’)
Note
If the cursor is in area (d) the notes for each procedure will be displayed (f).
1
a
e
1/1/2000
UPPER
LEVEL
REAGENTS
When all the selections are complete, press [REAGENT LAYOUT 2] to view the current
layout and optimise it if necessary.
4.2.7
10:42
UPPER
LEVEL
REAGENTS
18
A
^^
21 22 23 W1 W2 W3
H1
H2
H3
H4 H5
W4 W5 W6 24 25 26 B
LOWER
LEVEL
REAGENTS
Reagent Layout 2 Screen
LOWER
LEVEL
REAGENTS
C
The Shandon Varistain Gemini stainer allocates positions to all the reagents required for
every procedure selected. This screen displays detailed information about the reagents.
The screen (see page 43) is divided into five areas showing the following information:
1
2
3
4
5
6
7
8
9
10
11 12 13 14 15 16 17 18 19 20 D
UPPER LEVEL REAGENTS Set Times
Trough Reagent
Conc.%Uses Used
LOWER LEVEL REAGENTS Set Times
Trough Reagent
Conc.%Uses Used
A
Dry Storage
-
3
C
Xylene
21
Bluing Reagent
7
4
1
Xylene
22
Haematoxylin
23
Acid Alcohol
W1
W2
W3
-
3
7
*
2
Xylene
7
*
7
8
< 3
Xylene
7
8
Running Water
-
-
4
Alcohol
90
-
-
Running Water
-
-
5
Alcohol
70
-
-
Running Water
-
-
6
Alcohol
-
-
Haematoxylin
-
-
1
batches
PROCEDURES
Name / day Notes or staining steps
H&E
20
H&E
100
PAS
6
Notes for procedure
Dr xxxx procedure
Notes
<
7
a
Reagent layout - this shows the position of each reagent trough in the upper and
lower levels of the stainer.
A&B
1 - 26
H1 - H5
W1 - W6
C&D
Load positions / doors
Reagent troughs
Heater positions / dry storage
Water wash positions
Unload positions / doors
H1
Dry Storage
-
-
8
H2
Dry Storage
-
-
9
H3
Dry Storage
-
-
10
Alcoholic Eosin
-
-
H4
Dry Storage
-
-
11
Alcohol
-
-
H5
Dry Storage
-
-
12
Alcohol
-
-
13
Alcohol
-
-
14
W4
Running Water
-
-
15
Alcohol
-
-
W5
Running Water
-
-
16
Alcohol
-
-
W6
Running Water
-
-
17
Xylene
-
-
24
Periodic Acid
7
5
18
Xylene
7
5
25
Schiffs Reagent
7
4
19
Xylene
7
4
20
Xylene
7
4
D
Xylene
-
4
26
B
b
Details about the upper level reagents
c
Details about the lower level reagents
d
A list of the procedures entered in the ‘Reagent Layout 1’ screen.
e
Batch indicators showing the number and exact position of each batch that is
being processed.
42
1
Dry Storage
Print
A78010402 Issue 4
b
A78010402 Issue 4
-
4
100
Complete
Add Procedure
c
Optimise
d
f
43
Note
1 If the cursor is in area (b) or (c), details of the steps where the highlighted reagent is
used in each procedure will be displayed (g).
In the example shown, 100% alcohol in reagent troughs 9 and 15 are used in step 2 of
the H&E procedure, step 5 of the PAP and step 6 of the PAS procedure.
e
Reagent Layout 2 Function Buttons
The Function Buttons change depending on the status of the screen (see pages 43
and 44). This section describes all the Function Buttons that will be encountered in the
Reagent Layout 2 screen.
g
a
[ADD PROCEDURE] will go back to the ‘Reagent Layout 1’ screen to allow another
procedure to be added.
1/1/2000
UPPER
LEVEL
REAGENTS
UPPER
LEVEL
REAGENTS
18
^^
A
21 22 23 W1 W2 W3
H1
H2
H3
H4 H5
W4 W5 W6 24 25 26 B
LOWER
LEVEL
REAGENTS
LOWER
LEVEL
REAGENTS
C
1
2
3
4
5
UPPER LEVEL REAGENTS Set Times
Trough Reagent
Conc.%Uses Used
6
7
8
9
10
batches
PROCEDURES
Name / day Notes or staining steps
Dry Storage
-
3
C
Dry Storage
-
3
H&E
20
Step 2
21
Schiff’s Reagent
-
-
- 1
Xylene
-
-
PAP
100
Step 5
22
Haematoxylin
7
*
2
Xylene
7
*
PAS
6
Step 6
23
<
Acid Alcohol
7
8
3
Alcohol
7
8
4
Alcohol
90
-
-
W1
Running Water
-
-
5
Alcohol
70
-
-
W2
Running Water
-
-
6
Alcohol
-
-
W3
Running Water
-
-
7
H1
Dry Storage
-
-
8
9
Eosin
Alcohol
-
-
H2
Dry Storage
-
-
10
EA50
-
-
H3
Dry Storage
-
-
11
Alcohol
-
-
H4
Dry Storage
-
-
12
OG6
-
-
H5
Dry Storage
-
-
13
Alcohol
95
-
-
Alcohol
100
1
100
95
W4
Running Water
-
-
14
15
-
-
W5
Running Water
-
-
16
Xylene
-
-
W6
Running Water
-
-
17
Xylene
-
-
24
Acid Alcohol
7
5
18
Xylene
7
5
25
Haematoxylin
7
4
19
Xylene
7
4
26
Bluing Reagent 1
7
4
20
Xylene
7
4
B
Dry Storage
-
4
D
Xylene
-
4
1
Change Groups
44
[CHANGE GROUPS]. Press this Function Button to access the following three Sharing
Control screens - Reagent Sharing Control, Water Wash Sharing Control and Heater
Sharing Control.
11 12 13 14 15 16 17 18 19 20 D
LOWER LEVEL REAGENTS Set Times
Trough Reagent
Conc.%Uses Used
A
b
10:42
1
1
Move
These functions can be used to make sure that certain positions are not be shared
between different procedures, for example with gynaecological and non-gynaecological
procedures.
<
Note
1 A group is a set of troughs that are used interchangeably. Groups may be
created to improve performance or to extend reagent life
Place the cursor on a reagent position and press [CHANGE GROUPS] to display the
‘Reagent Sharing Control’ screen.
Place the cursor on a water wash position and press [CHANGE GROUPS] to display
‘Water Wash Sharing Control’ screen.
Duplicate
c
d
Place the cursor on a heater position (heater version only) and press
[CHANGE GROUPS] to display the ‘Heater Sharing Control’ screen.
A78010402 Issue 4
A78010402 Issue 4
45
a
‘Reagent Sharing Control’ screen
b
This screen shows all the water wash stations and which steps of the procedures use
them.
This screen shows information about the sharing of reagents:
i
ii
The reagent that was selected when
[CHANGE GROUPS] was pressed
(b)
All the procedures that use that
reagent (a)
a
e
d
c
b
‘Water Wash Sharing Control’ screen
Reagent Sharing Control
1/1/2000
10:42
If ‘xxx’ is displayed, that Water Wash
Station is to be used. If nothing is
displayed the Water Wash Station will not
be used.
Water Wash Sharing Control
RUNNING WATER STEPS
No
Procedure Name & Notes Step
iii
iv
v
The steps where the reagent is
used (c)
The troughs that contain the reagent
(d)
Procedure Name & Notes
1
H&E
2
PAS
Step
3
2
3
4
xxx
3
5
6
xxx
7
In the example, Water Wash Station W2
will not be used for H&E step 10, and W1
will not be used for PAS step 12.
12
xxx
xxx
xxx
To change the setting for the Water Wash
Stations, use the direction arrows to the
particular step and Water Wash Station,
and press [USE] or [DO NOT USE].
Any spare troughs (e).
Reagent Sharing Control
Only one spare trough will be
displayed at any one time. If there
are no spare troughs available, the
spare trough box (e) will not be
displayed.
8
10
12
14
16
xxx
xxx
xxx
xxx
xxx
xxx xxx
xxx
xxx xxx
xxx xxx
xxx
xxx
xxx
xxx
xxx
xxx
xxx
xxx
xxx
xxx
xxx
2
PAS
8
10
12
15
17
xxx xxx xxx
xxx xxx xxx
xxx xxx
xxx xxx xxx
xxx xxx xxx
xxx
xxx
xxx
xxx
xxx
xxx xxx
xxx xxx
xxx xxx
xxx
xxx xxx
To make sure that a particular trough of
reagent is not shared between procedures,
use [
] and [
] to highlight the trough
to be changed and move the ‘xxx’ to the
spare trough using [
] and [
].
1/1/2000
Use
Do Not Use
10:42
-%
No
Procedure Name & Notes
1
H&E
PAS
Step
3
2
3
4
xxx
3
5
6
xxx
7
c
12
‘Heater Sharing Control’ screen
This screen works in the same way as the ‘Water Wash Sharing Control’ screen for the
allocation of heater stations on the heater version of the Shandon Varistain Gemini.
xxx
xxx
xxx
xxx
The ‘Heater Sharing Control’ screen also controls the use of ‘dry storage’ steps (if
selected) on both versions on the Shandon Varistain Gemini.
Reagent Sharing Control
1/1/2000
10:42
GROUPS OF REAGENT POTS
Pre-Stain Xylene
-%
No
Procedure Name & Notes
1
H&E
2
PAS
Step
3
2
3
4
xxx
3
5
6
xxx
7
[CHANGE OPTIONS] is displayed when the door positions are highlighted. If pressed,
the ‘Reagent Layout 1’ screen will be shown.
12 10
[COMPLETE]. Press this Function Button when you are satisfied with the reagent
layout, before you move on to the ‘Load Reagents’ screen.
xxx
xxx
xxx
xxx
[DELETE] is only available if any duplicates are highlighted in the ‘Reagent Layout
2’ screen. It removes a selected duplicate reagent from the layout. It will only remove
reagents that are duplicated, and will not delete the last trough of any reagent group.
The instrument will automatically allocate a spare trough for this reagent.
In the example, step 5 of the PAS procedure has been reallocated to trough 10.
46
xxx
xxx
xxx
xxx
xxx
GROUPS OF REAGENT POTS
Pre-Stain Xylene
2
The example screen shown shows that
Xylene (b) is used in steps 2, 3 and 4 of
the H&E procedure and steps 3, 5 and 6
of the PAS procedure (a & c). Troughs 3, 7
and 12 contain Xylene (d) and there is at
least one spare trough (e).
W4 W5 W6
xxx
Note
1
W1 W2 W3
H&E
-%
No
10:42
1
GROUPS OF REAGENT POTS
Pre-Stain Xylene
1/1/2000
WATER WASH STATIONS
A78010402 Issue 4
In the example shown on page 44, either reagent 9 or reagent 15 can be deleted, but
not both.
A78010402 Issue 4
47
[DUPLICATE] allows a reagent to be copied to an empty position. The original reagent
and any duplicates will be grouped, and will be shared for any steps where that reagent
is required.
WARNING
Refer to ‘Safety and Chemicals’ section on page 7 before the instrument is
filled with reagents.
DO NOT FILL HEATER STATION TROUGHS WITH REAGENTS.
In the example shown, the 100% alcohol in position 9 has been copied to position 15.
The steps where this reagent is used will now use either trough 9 or trough 15.
DO NOT SPLASH OR POUR REAGENTS NEAR TO THE HEATER STATION
TROUGHS.
[MOVE] allows a reagent to be moved to an empty position if required.
DO NOT PLACE STANDARD REAGENT TROUGHS INTO THE HEATER
POSITIONS - REAGENTS MAY BE INADVERTENTLY HEATED AND THE
INSTRUMENT MAY NOT OPERATE CORRECTLY.
[OPTIMISE]. Press this Function Button to access the following three Function Buttons:
[AUTO ALLOCATE] first removes all existing duplicates. Then it adds the best
duplicates in positions to optimise the performance of the instrument for a day’s
work.
a
Reagent Monitoring Screen
Note
1
b
c
Before any procedures can be run,
the reagents allocated by the Reagent
Allocation programme must be loaded into
the instrument in the correct positions.
If a day’s work can be carried out without a replenishment stop or any
bottlenecks, [AUTO ALLOCATE] will not make any changes and may leave
some troughs empty.
[AUTO ARRANGE] optimises the positions of the current reagents for the best
possible performance. It will not add or remove duplicates.
[REMOVE DUPLICATES] optimises the reagent layout for the cheapest option.
All duplicates are removed - only one trough of each required reagent is left.
From the ‘How To Stain’ screen, press
[LOAD REAGENTS] to enter the ‘Reagent
Monitoring’ screen.
1/1/2000
10:42
Quick Reference for using the Varistain Gemini stainer
1.Use DEFINE REAGENTS to enter the names of reagents to be used
2.Use DEFINE PROTOCOLS to enter the parameters for each program step
3.Use REAGENT LAYOUT to select the procedures currently required
4.Use LOAD REAGENTS to confirm each reagent has been placed in position
5.To stain a batch of slides open the Load Door and follow the on screen
instructions for selecting the procedure required
6.When a batch has completed its staining protocol it will be placed behind an
unload station door and its acknowledge button light will flash. Open the door
and follow the instructions on the screen.
Define Reagents
Define Protocols
Reagent Layout
Load Reagents
The ‘Reagent Monitoring’ screen (see page 50) shows the following information:
i
The exact positions of each batch of slides (a)
ii
‘<’ shows if any reagent has exceeded its set number of uses (b)
iii
The positions where each reagent must be loaded to ensure correct operation of
the instrument (c)
iv
‘>’ shows that a reagent has not been loaded in position (i.e. [CONFIRM LOADED]
has not been pressed) (d)
v
How many weeks the Charcoal Filter has been installed (e)
[PRINT] will print the reagent layout if a printer is connected.
4.2.8
Varistain Gemini
How to Stain
To Load Reagents
USE GOOD LABORATORY PRACTICE WHEN HANDLING REAGENTS. BE
AWARE OF ANY HAZARDS ASSOCIATED WITH THEM. ALWAYS USE COMMON
SENSE.
48
A78010402 Issue 4
A78010402 Issue 4
49
To Load Reagents into the Shandon Varistain Gemini
Carefully pour the required reagent into a reagent trough to the marked fill line.
a
a
Note
1 The reagent troughs will hold
320ml of reagent - enough to
cover 1 - 20 slides.
b
Reagent Monitoring
UPPER
LEVEL
REAGENTS
UPPER
LEVEL
REAGENTS
A
21
22
23
C
1
2
3
W1 W2 W3
H1
H2
H3
H4
H5
W4 W5 W6 24
25
26
B
19
20
D
LOWER
LEVEL
REAGENTS
Reagent Monitoring
1/1/2000
UPPER
LEVEL
REAGENTS
UPPER
LEVEL
REAGENTS
18
A
^^
21 22 23 W1 W2 W3
H1
H2
H3
H4 H5
W4 W5 W6 24 25 26 B
LOWER
LEVEL
REAGENTS
LOWER
LEVEL
REAGENTS
C
1
2
3
4
5
6
7
8
9
10
11 12 13 14 15 16 17 18 19 20 D
UPPER LEVEL REAGENTS Set Times
Trough Reagent
Conc.%Uses Used
LOWER LEVEL REAGENTS Set Times
Trough Reagent
Conc.%Uses Used
batches
PROCEDURES
Name / day Notes or staining steps
A>
Dry Storage
C>
Xylene
H&E
20
21>
Bluing Reagent
7
4
1>
Xylene
H&E
100
22>
Haematoxylin
7
4
2>
Xylene
PAS
6
23>
Acid Alcohol
7
8
3>
Xylene
W1
Running Water
-
-
4>
Alcohol
90
W2
Running Water
-
-
5>
Alcohol
70
W3
Running Water
-
-
6>
Alcohol
H1
Dry Storage
-
-
H2
Dry Storage
-
-
H3
Dry Storage
-
-
H4
Dry Storage
-
-
H5
Dry Storage
-
-
W4
Running Water
-
-
W5
Running Water
-
-
W6
Running Water
-
-
24>
Periodic Acid
7
5
25>
Schiffs Reagent
7
4
1
7
*
7
8
-
-
-
-
-
-
Haematoxylin
-
-
10>
Alcoholic Eosin
-
-
11>
Alcohol
-
-
12>
Alcohol
-
-
13>
Alcohol
-
-
1
26>
B>
Dry Storage
Place the trough in the correct position
in the instrument.
Notes
1 The position number of each
reagent trough is marked in the
instrument.
2
Notes for procedure
Dr xxxx procedure
Notes
Rotate the Turntable when the
lower level reagents are loaded,
taking care to avoid spillage.
5
Alcohol
-
-
16>
Alcohol
100
-
-
17>
Xylene
-
-
18>
Xylene
7
5
19>
Xylene
7
4
20>
Xylene
7
4
D>
Xylene
8
C>
Xylene
7
4
1>
Xylene
22>
Haematoxylin
7
4
2>
Xylene
Acid Alcohol
1
7
10
11
12
LOWER LEVEL REAGENTS
Set
Trough Reagent
Conc.%Uses
Times
Used
Dry Storage
23>
9
3>
8
13
14
QUALITY CONTROL WARNING
The Procedure selected cannot be run without exceeding
the limits set within the Define Procedures screen, for
reagents marked <.
Xylene
7
8 <
Running Water
-
-
4>
Alcohol
90
-
-
-
-
5>
Alcohol
70
-
-
W3
Running Water
-
-
6>
Alcohol
-
-
7
8>
-
Haematoxylin
-
-
H2
Dry Storage
-
-
9
H3
Dry Storage
-
-
10>
Alcoholic Eosin
-
-
H4
Dry Storage
-
-
11>
Alcohol
-
-
H5
Dry Storage
-
-
12>
Alcohol
-
-
13>
W4
Running Water
-
Running Water
-
-
Running Water
-
-
Periodic Acid
Schiffs Reagent
1
7
5
7
4
Alcohol
Alcohol
17>
Dry Storage
Cancel
15>
16>
18>
26>
B>
Alcohol
-
To replenish reagents use the arrow keys to select the
reagents and press CLEAR after each replenishment.
Press CONTINUE when all warnings have been
cleared. Press OVERRIDE to ignore any
remaining
warnings. The stainer will restart automatically, including the latest batch.
Pressing RESTART will only continue staining the interrupted batches. Press CANCEL to abort the loading
of the latest batch.
-
14
-
W5
W6
18
*
Running Water
-
17
<<<<<<< QUALITY CONTROL WARNING >>>>>>>
7
W1
Dry Storage
16
Times
Used
W2
H1
15
Xylene
Xylene
100
-
-
-
-
7
5
19>
Xylene
7
4
20>
Xylene
7
4
D>
Xylene
Confirm Loaded
HARDWARE QUALITY COUNTS
Limit
13
Charcoal Filter Replacement
Count
14 <
Override
<
QUALITY CONTROL WARNING
As each reagent is placed in position, press
[CONFIRM LOADED] to acknowledge that the reagent
is in the correct position. (The ‘>’ displayed next to each
reagent position will disappear as soon as the reagent
has been acknowledged. (See (c) on ‘Reagent Monitoring
screen on page 50))
<<<<<<< QUALITY CONTROL WARNING >>>>>>>
The Procedure selected cannot be run without exceeding the limits set within the Define
Procedures screen, for reagents marked <.
To replenish reagents use the arrow keys to
select the reagents and press CLEAR after each
replenishment.
Press CONTINUE when all warnings have
been cleared. Press OVERRIDE to ignore any
remaining warnings. The stainer will restart
automatically, including the latest batch.
REAGENTS MUST BE PLACED IN THE CORRECT
POSITIONS AND [CONFIRM LOADED] PRESSED
FOR THE INSTRUMENT TO WORK CORRECTLY.
HARDWARE QUALITY COUNTS
Charcoal Filter Replacement
Stop
Limit
13
Count
14 <
Batch Monitoring
e
Pressing RESTART will only continue staining the
interrupted batches. Press CANCEL to abort the
loading of the latest batch.
Note
1
d
7
Bluing Reagent
14
15>
6
A>
24>
9
Confirm Loaded
c
UPPER LEVEL REAGENTS
Set
Trough Reagent
Conc.%Uses
4
21>
25>
7
8>
LOWER
LEVEL
REAGENTS
10:42
If the loading of reagents has not been confirmed before the procedure is
started, the red Quality Control Warning screen (see Section 4.2.11) will
automatically be displayed. A ‘>’ next to a reagent means that the loading of that
reagent has not yet been confirmed.
When the cursor is in either of the reagent boxes (a), press [CONFIRM LOADED] to
confirm the reagent.
50
A78010402 Issue 4
A78010402 Issue 4
51
Batch Monitoring Screen
iii
The time each batch started (c)
From the ‘Reagent Monitoring’ screen, press [BATCH MONITORING] to enter the
‘Batch Monitoring’ screen.
iv
The procedure and procedure number being used to stain each batch of slides
(d)
v
The procedure step that the batch has reached (e)
vi
The reagent that the batch has reached (f)
The ‘Batch Monitoring’ screen will be used regularly to view the progress of each batch
of slides. The following information is displayed:
i
The batch numbers of the slides that are currently being stained (a)
vii The time remaining at that step (g)
ii
The exact positions of each batch of slides (b)
viii The trough position where the batch of slides has reached (h)
b
Batch Monitoring
1/1/2000
UPPER
LEVEL
REAGENTS
^^
21 22 23 W1 W2 W3
LOWER
LEVEL
REAGENTS
H1
H2
H3
H4 H5
W4 W5 W6 24 25 26 B
LOWER
LEVEL
REAGENTS
19
C
^^
1
2
Batch
#
Start
Time
Procedure
18
19
10:55
11:10
1
2
c
UPPER
LEVEL
REAGENTS
18
A
a
10:42
H&E
PAS
d
3
4
5
Step
7
2
e
6
7
8
9
10
11 12 13 14 15 16 17 18 19 20 D
Reagent
Time
Pot
End Time & Place
Priority
Running Water
Xylene
1:23
0:50
W3
2
11:21
11:40
Standard
Standard
f
g
h
i
D
C
j
k
ix
The time when the batch should finish its procedure (i)
x
The output door where the batch will finish (j)
xi
The priority of the batch (k)
Note
1 When loading another batch of
slides, if the instrument calculates
that during a multiple batch run, a
reagent will exceed its set uses, the
red Quality Control Warning Screen
(see Section 4.2.11) will be displayed.
The reagent to be replenished will be
marked with a ‘<’ next to it. Follow the
on-screen instructions to replenish
the reagent. Once the reagent has
been replenished, press [CLEAR].
Reagent Monitoring
A
21
22
23
C
1
2
3
Auto Return
52
Stop
Reagent Monitoring
A78010402 Issue 4
A78010402 Issue 4
UPPER
LEVEL
REAGENTS
W1 W2 W3
H1
H2
H3
H4
H5
W4 W5 W6 24
25
26
B
19
20
D
LOWER
LEVEL
REAGENTS
UPPER LEVEL REAGENTS
Set
Trough Reagent
Conc.%Uses
A>
Dry Storage
21>
Bluing Reagent
22>
23>
7
Haematoxylin
Acid Alcohol
7
1
7
4
Times
Used
4
4
8
5
6
7
8
9
10
11
Xylene
Xylene
2>
3>
4>
Xylene
7
Xylene
7
Alcohol
90
Running Water
-
-
5>
Alcohol
70
Running Water
-
-
6>
Alcohol
Running Water
-
-
13
14
H1
H2
Dry Storage
-
Dry Storage
-
-
H3
Dry Storage
-
-
Dry Storage
-
-
H5
Dry Storage
-
-
8>
Running Water
-
-
Running Water
-
-
Running Water
-
-
24>
25>
Periodic Acid
Schiffs Reagent
Dry Storage
Cancel
1
7
5
7
4
17
18
QUALITY CONTROL WARNING
*
The procedure selected cannot be run without exceeding
the limits set within the Define Procedures screen, for
reagents marked <.
-
8 <
-
-
-
-
-
To replenish reagents press STOP and follow the onscreen prompts. When the stainer has stopped use the
arrow keys to select reagents and press CLEAR after
each replenishment.
Press CONTINUE when all warnings have been cleared.
Haematoxylin
-
-
9
10>
Alcoholic Eosin
-
-
11>
Alcohol
-
-
12>
Alcohol
-
-
13>
W4
W5
W6
16
Times
Used
7
H4
15
<<<<<<< QUALITY CONTROL WARNING >>>>>>>
W2
W1
12
LOWER LEVEL REAGENTS
Set
Trough Reagent
Conc.%Uses
C>
1>
W3
26>
The count will not necessarily be
reset to zero. The number displayed
on the red Quality Control Warning
screen is the total number of times
that a reagent trough will have been
used by the time all batches have
completed their staining procedure
(including the batch being loaded).
If [CLEAR] is pressed, all the uses
that have already happened will be
removed, but those that are still to
occur are left.
10:42
18
^^
LOWER
LEVEL
REAGENTS
B>
2
1/1/2000
UPPER
LEVEL
REAGENTS
Alcohol
-
Press OVERRIDE to remove the warning screen and
continue to stain using the current reagents.
-
14
15>
Alcohol
100
-
-
16>
Alcohol
-
-
17>
Xylene
-
-
18>
19>
Xylene
7
5
Xylene
7
4
20>
Xylene
7
4
D>
Xylene
Clear
HARDWARE QUALITY COUNTS
Limit
13
Charcoal Filter Replacement
Restart
Count
14 <
Override
QUALITY CONTROL WARNING
<<<<<<< QUALITY CONTROL WARNING >>>>>>>
The procedure selected cannot be run without exceeding the limits set within the Define
Procedures screen, for reagents marked <.
To replenish reagents press STOP and follow
the on-screen prompts. When the stainer has
stopped use the arrow keys to select reagents
and press CLEAR after each replenishment.
Press CONTINUE when all warnings have been
cleared.
Press OVERRIDE to remove the warning screen
and continue to stain using the current reagents.
53
4.2.9
To Stain Slides
[AUTO RETURN] will return a specified batch to the trough behind the load door.
Use the direction arrows to move the cursor to highlight the required batch and press
[AUTO RETURN].
Once all the reagents have been correctly placed in the stainer, slides can be stained.
Note
1 The [AUTO RETURN] button on the Load / Unload Touch Panel (paragraph
3.3.3) will return only the last batch of slides loaded onto the instrument.
The Load Acknowledge LED (on the Load
Buttons [A] and [B]) will be lit if that load
door can be used (a).
Varistain Gemini
1/1/2000
10:42
Door A - Loading Slides - Batch 57
[STOP] (on both ‘Batch Monitoring’ and ‘Reagent Monitoring’ screens) is used to stop
a batch of slides. The instrument will finish its current movement and will then stop the
time countdowns in all procedures to allow the batch to be retrieved.
A
C
AUTO
RETURN
Open the sliding door at either A or B
and place a basket and carrier into the
loading trough directly behind the door
(marked A and B on the base adjacent
to the troughs). The ‘Loading’ screen will
automatically appear on the monitor. Use
the direction arrows [
] and [
] to
select the required procedure.
B
D
To use [STOP]:
A
C
AUTO
RETURN
PROCEDURE
Note for selected procedure
No.Procedure name / Notes
4
PROCEDURE Note for selected procedure
1
PAP
Dr xxxx special PAP
Close the load door, select the required
procedure and press the appropriate START.
Standard Start
Urgent Start
Set As Default
Cancel
1
Wait until the instrument places all the batches of slides in safe stop positions.
2
Press [STOP].
3
Press [STOP NOW] to confirm that you do want to stop the instrument, or
[DO NOT STOP] to return to the ‘Batch Monitoring’ screen.
THE SLIDING DOORS ARE ONLY INTENDED FOR THE OPERATOR TO ACCESS
THE LOAD AND UNLOAD TROUGHS DIRECTLY BEHIND THE DOORS. DO NOT
REACH FURTHER INTO THE INSTRUMENT - THE ARM CAN MOVE SUDDENLY.
4
Use the direction arrows to select the
batch required and press [ABORT] when
the slides are in a convenient position to
be removed from the instrument.
THE ARM AND BASKET CAN MOVE SUDDENLY BOTH HORIZONTALLY AND
VERTICALLY.
B
D
A
C
AUTO
RETURN
B
D
DO NOT REST HANDS ON TOP OF THE TROUGHS.
ANY BATCHES THAT ARE ABORTED
DURING A PROCEDURE MUST BE
REMOVED FROM THE INSTRUMENT.
A
C
5
6
AUTO
RETURN
B
D
A
C
AUTO
RETURN
DO NOT TOUCH THE ARM MECHANISM IN THE CENTRE OF THE INSTRUMENT.
A
C
AUTO
RETURN
B
D
A
C
AUTO
RETURN
B
D
Press [RESTART] to resume the staining
procedures of all other batches in the
instrument.
A
C
AUTO
RETURN
Alternatively, select [STANDARD START] or [URGENT START] from the ‘Loading’
screen.
a
B
D
Note
1 A commonly used procedure can be set as the default program. This allows the
operator to open one of the load doors, A or B, load the slides, close the door
and press either [A] or [B] on the Load / Unload Panel to acknowledge.
CHECK THAT EVAPORATION COVERS
ARE REMOVED FROM ALL TROUGHS
BEFORE USE.
54
B
D
Close the doors and if the procedures are set up, press [A] or [B] on the Load / Unload
Panel to acknowledge.
B
D
Remove the correct batch from the
instrument and close the doors.
A
C
AUTO
RETURN
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4.2.11 Quality Control Warning Screen
The default procedure will start automatically on Standard or Urgent, depending
on the Load Door Option selected in the ‘Reagent Layout 1’ screen.
The Quality Control Warning Screen is automatically displayed whenever there is
a conflict that will prevent the instrument loading a batch of slides and staining it
according to its set uses for each reagent.
To set a procedure as the default procedure, move the cursor using the direction
arrows [
] and [
]. Press [SET AS DEFAULT] in the ‘Loading’ screen.
The default procedure will be highlighted when the ‘Loading’ screen is first
displayed. If no procedure is set as default, the first procedure on the list will be
highlighted.
A
C
AUTO
RETURN
B
D
A
C
AUTO
RETURN
A
C
AUTO
RETURN
B
D
Follow the instructions given in the Quality Control Warning box to clear the conflict.
An example is shown here.
B
D
4.2.10 To Unload Slides
Note
If a ‘<’ is displayed in other screens, then a Quality Control conflict will exist by
the time the batch to be loaded has run through its procedure.
1
Once a batch of slides has been stained and
the batch of slides is ready to be removed from
the instrument, the Unload Acknowledge LED
on the relevant Unload Button ([C] or [D]) will
flash (a) to show which door should be opened
to retrieve the slides.
Reagent Monitoring
10:42
1/1/2000
UPPER
LEVEL
REAGENTS
UPPER
LEVEL
REAGENTS
18
^^
A
21
22
23
C
1
2
3
H1
W1 W2 W3
H3
H2
H4
H5
W4 W5 W6 24
25
26
B
18
19
20
D
LOWER
LEVEL
REAGENTS
LOWER
LEVEL
REAGENTS
A
C
In the example shown, the LED on Unload
Button [C] is flashing (a). The batch of slides in
this case is in the trough behind Unload Door
[C].
A
C
A
C
AUTO
RETURN
AUTO
RETURN
B
D
A
C
AUTO
RETURN
AUTO
RETURN
B
D
A
C
AUTO
RETURN
B
D
Set
UPPER LEVEL REAGENTS
Conc.%Uses
Trough Reagent
B
D
B
D
A>
Dry Storage
21>
Bluing Reagent
22>
Haematoxylin
23>
Acid Alcohol
W1
Running Water
W2
W3
4
Times
Used
6
5
7
8
9
10
11
Set
LOWER LEVEL REAGENTS
Conc.%Uses
Trough Reagent
C>
Xylene
14
13
Times
Used
4
1>
Xylene
7
4
2>
Xylene
7
8
3>
Xylene
-
-
4>
Alcohol
90
Running Water
-
-
5>
Alcohol
70
Running Water
-
-
6>
Alcohol
H1
Dry Storage
-
-
8>
H2
Dry Storage
-
-
9
H3
Dry Storage
-
-
10>
H4
Dry Storage
-
-
H5
Dry Storage
-
-
W4
Running Water
-
-
W5
Running Water
-
-
W6
Running Water
-
-
24>
Periodic Acid
7
5
25>
Schiffs Reagent
7
4
7
*
7
8 <
-
-
-
-
-
-
Haematoxylin
-
-
Alcoholic Eosin
-
-
11>
Alcohol
-
-
12>
Alcohol
-
-
13>
Alcohol
-
-
7
Open the Unload Door and remove the batch
of slides. Press the relevant Unload Button
to acknowledge that the slides have been
removed and that the Unload Door is now free
for another batch.
A
C
AUTO
RETURN
B
D
a
1
26>
B>
Dry Storage
Cancel
Alternatively, [ACKNOWLEDGE]
pressed in the ‘Unload’ screen.
56
can
15
16
17
QUALITY CONTROL WARNING
QUALITY CONTROL WARNING
<<<<<<< QUALITY CONTROL WARNING >>>>>>>
7
1
12
The Procedure selected cannot be run without exceeding
the limits set within the Define Procedures screen, for
reagents marked <.
To replenish reagents use the arrow keys to select the reagents and press CLEAR after each replenishment.
Press CONTINUE when all warnings have been cleared.
remaining warnPress OVERRIDE to ignore any
automatically, including
ings. The stainer will restart
the latest batch.
Pressing RESTART will only continue staining the interrupted batches. Press CANCEL to abort the loading
of the latest batch.
<<<<<<< QUALITY CONTROL WARNING >>>>>>>
The Procedure selected cannot be run without exceeding the limits set within the Define
Procedures screen, for reagents marked <.
To replenish reagents use the arrow keys to
select the reagents and press CLEAR after each
replenishment.
14
15>
Alcohol
-
-
16>
Alcohol
-
-
17>
Xylene
-
-
18>
Xylene
7
5
19>
Xylene
7
4
20>
Xylene
7
4
D>
Xylene
Confirm Loaded
100
HARDWARE QUALITY COUNTS
Limit
13
Charcoal Filter Replacement
Override
Count
14 <
Press CONTINUE when all warnings have been
cleared. Press OVERRIDE to ignore any remaining warnings. The stainer will restart automatically, including the latest batch.
Pressing RESTART will only continue staining the
interrupted batches. Press CANCEL to abort the
loading of the latest batch.
be
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57
4.3 HOUSEKEEPING TASKS
Varistain Gemini
Options
Select [OPTIONS] from the Main Menu to
access the Housekeeping tasks.
4.3.1
Date
To Change the Time and
1/1/2000
10:42
The Charcoal filter life warning level can
be set in this screen (in weeks). It is
recommended that the filter is replaced at
least every 13 weeks.
Varistain Gemini
Quality Control
Please select the required action
1/1/2000
Reagent Usage Warning
Press SET CLOCK to alter the time or date.
Press WARNINGS for Quality Control and audible warnings.
Press DATA MANAGEMENT to use the disc drive or to obtain printouts.
Press ACCESS CODES to set or alter the protection code.
If the filter has exceeded the limit set, a
warning will be displayed at the start of
each staining run until the filter is replaced.
Filter Replacement Warning Interval
10:42
On
13 weeks
Press [SET CLOCK] to display the ‘Set
Clock’ screen.
Set Clock
Warnings
Data Management
Access Code
Press [12 / 24 Hour Clock] to switch
between 12 and 24 hour clock format.
Varistain Gemini
Press [DAY <-> MONTH] to switch
between the date options of Day / Month
or Month / Day.
Use the direction arrows to move to the
time and date settings to change them.
Use the number keypad on the Power
Supply Unit to enter the new time and
date.
Set Clock
1/1/2000
10:42
Select display format
Time
Date
24 Hour
Day / Month
Hours
Minutes
Day
Month
Year
10
42
1
1
2000
4.3.3
On / Off
To Change the Audio Setup
Select [WARNINGS] and then select
[AUDIO].
Varistain Gemini
Select time and date
Audio Configuration
Press SET to change the clock to the new values
Set
12/24 Hour Clock
Day <-> Month
Note
1 The ‘Set Clock’ screen cannot be accessed while a staining procedure is in
progress.
4.3.2
Press [ON / OFF] to turn the warning off if
a fume extraction adaptor is fitted.
Quality Control Settings
1/1/2000
10:42
Use the direction arrows to select the
required event.
Please select the required option and then press DEMONSTRATE
to check the audible warning given when each event occurs. Press
NEW TUNE to alter the selection. Press Silence to turn off the audible
warning for that event.
Press [DEMONSTRATE] to hear the
warning sound chosen.
1
2
3
4
5
6
7
8
9
Press [NEW TUNE] to select a new
warning sound. A selection of warning
sounds will be displayed.
Event
Sound
Start Up Complete
Signature Tune
A keypanel key is pressed
Short blip
A load door is left open for 15 seconds
4 falling notes
A load door is left open for 30 seconds
4 falling notes
A batch finishes staining
4 rising notes
A Load / Unload door is opened in error 4 falling notes
A Basket Transport Arm error occurs
Error notes
A Main Door is opened in error
Error notes
Load sequence incomplete for 30 seconds 4 falling notes
Demonstrate
New Tune
Silence
Repeats
0
0
2
10
0
0
Continuous
Continuous
2
Continuous
Press [SILENCE] to turn off the warning sound for the chosen event.
Select [WARNINGS] and then select [QUALITY CONTROL].
Press [CONTINUOUS] to set the number of repeats to continuous. The warning sound
will continue until [
] on the PSU is pressed.
Press [ON / OFF] to turn the Reagent Usage warnings on or off.
If ‘On’ is chosen, warnings are shown on the ‘Reagent Monitoring’ screen if any of the
reagents have reached the usage limits set when the procedure was defined.
Note
1
58
A78010402 Issue 4
To remove ‘Continuous’ from the repeat column, enter another number.
A78010402 Issue 4
59
4.3.4
To Print Information
Select [OPTIONS] from the Main Menu,
and then select [DATA MANAGEMENT].
Information can be printed out on an IBM
compatible Centronics parallel printer.
Select [DISC DRIVE] from the Options,
Data Management screen.
Varistain Gemini
Data Management
Select [OPTIONS] from the Main Menu,
and then select [DATA MANAGEMENT].
1/1/2000
10:42
Use the direction arrows [
Please select the required action
Press DISC DRIVE to store or retrieve data using a floppy disc.
Select [PRINTOUTS] from the Options,
Data Management screen.
Press PRINTOUTS to obtain printouts of Event Logs, Procedures and
Reagent Layouts. All printouts can be previewed on screen before printing
Use the direction arrows [
] and [
]
or the number keys to select the required
option, and then press the correct function
key to choose the required destination for
the information:
Disc Drive
] and [
]
or the number keys to select the required
1/1/2000
10:42
Please select the required option and then press START
1
2
3
4
5
6
7
8
9
Write a copy of a Procedure to a floppy disc
Write all Procedures to a floppy disc
Write Reagent Layout and Door Configuration to a floppy disc
Write the entire Instrument Setup to a floppy disc
Read a Procedure from a floppy disc
Read all Procedures from a floppy disc
Read Reagent Layout and Door Configuration from a floppy disc
Read the entire Instrument Setup from a floppy disc
Load new features from a floppy disc
option, and then press [START] to access
the disc drive.
Start
Note
Printouts
1
4.3.6
Note
1
Varistain Gemini
Disc Drive Options
[PRINTER] will print the information.
2
[ON-SCREEN] will display the information on the monitor
3
[DISC DRIVE] will save the information to disc in the form of a text file that
can be read and edited using a standard PC text editor. (Use standard DOS
compatible 1.44Mb 31⁄2 inch floppy disks.)
The file saved can only be used by a Shandon Varistain Gemini. If a record of the
instrument settings is required, choose the Printouts option.
Access Codes
Access codes can be used as a security
measure
when
programming
the
instrument, changing the Reagent Layout
or items 6 - 9 from the Disc Drive Options
menu.
Varistain Gemini
Access Codes
1/1/2000
10:42
The Access Code is not set
Select [OPTIONS] from the Main Menu,
and then select [ACCESS CODES].
Varistain Gemini
4.3.5 To
Save
or
Retrieve
Information From a Floppy Disk
The Shandon Varistain Gemini stainer has
a 31⁄2 inch disk drive to allow the operator
to save particular procedures or records
of specific batches of slides, and also
to download standard procedures and
information to set up the instrument.
Printout Options
1/1/2000
10:42
To set an access code, press [SET
ACCESS CODE].
Set Access Code
Please select the required option and then press PRINTER, ON-SCREEN
or DISC DRIVE.
1
2
3
4
5
6
Use the number keypad to enter a 4 digit code. Press [ENTER]. Re-enter the code
followed by [ENTER] when prompted. The access code is now active.
Print a copy of a Procedure
Print all Procedures
Print Reagent Layout and Door Configuration
Print Event Log for a specific batch of slides
Print Event Logs for all batches of slides
Print the entire Instrument Setup
To change an access code, press [SET ACCESS CODE] and enter the old access
code followed by [ENTER].
Use standard DOS compatible 1.44Mb 31⁄2
inch floppy disks.
60
Printer
On-Screen
Disc Drive
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61
Enter a new 4 digit code followed by
[ENTER] when prompted. This new code
must be entered again before the access
code is changed.
Access Codes
1/1/2000
10:42
For the continued safe and efficient operation of the instrument, it is important that
regular inspection of the instrument during the following cleaning and maintenance
procedures is carried out.
The Access Code is set
To clear an access code, enter the old
code and press [ENTER] twice when
prompted for a new code.
If you forget the password, press
[CANCEL ACCESS CODE] and wait for
2 days.
CLEANING AND MAINTENANCE
Varistain Gemini
IF HAZARDOUS MATERIAL IS SPILT ON, OR INSIDE, THE INSTRUMENT, THE
USER SHOULD CARRY OUT THE APPROPRIATE DECONTAMINATION.
Set Access Code
CLEANING OR DECONTAMINATION METHODS, OTHER THAN THOSE
RECOMMENDED IN THE OPERATOR GUIDE, SHOULD BE CHECKED WITH
A THERMO AGENT TO ENSURE THAT THE PROPOSED METHOD WILL NOT
DAMAGE THE EQUIPMENT.
Cancel Access Code
Notes
1
The time will count down on the top of each screen.
2
To keep the access code and stop the countdown, press [ * ] from any screen.
5.1 CLEANING
TURN OFF THE POWER AND REMOVE THE MAINS PLUG FROM THE MAINS
SUPPLY BEFORE CLEANING THE SHANDON VARISTAIN GEMINI STAINER.
To use the access code, enter the code whenever the screen requests it. Once the
access code has been used, press [ * ] to re-enable it.
ALWAYS WIPE UP ANY SPILLS IMMEDIATELY. IN THE EVENT OF A MAJOR
SPILLAGE, DISCONNECT THE INSTRUMENT FROM THE MAINS SUPPLY
WITHOUT DELAY AND DO NOT RECONNECT AND SWITCH ON UNTIL THE
INSTRUMENT HAS BEEN THOROUGHLY DRIED OUT AND CHECKED BY A
SERVICE ENGINEER.
5.1.1
Cleaning the Stainer
Clean the instrument regularly as part of your normal housekeeping routine.
WARNING
Any accidental spillage of stains on the touch control panel should be
removed by wiping with alcohol immediately.
To clean the inside and outside of the Shandon Varistain Gemini stainer, wipe with a
cloth damped in soapy water, then polish with a dry cloth. DO NOT USE EXCESSIVE
LIQUID.
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63
5.1.2
TROUBLE SHOOTING
Cleaning the Power Supply Unit
The case of the Power Supply Unit only requires wiping with a clean, dry cloth.
Any accidental spillage of stains on the touch control panel should be
WARNING removed by immediately wiping with a cloth and a small amount of alcohol.
POTENTIALLY LETHAL VOLTAGES ABOVE 110V A.C. ARE PRESENT INSIDE
THE POWER SUPPLY UNIT. DO NOT REMOVE ANY ACCESS COVERS.
5.1.3
Cleaning the Reagent Troughs
The plastic reagent and water wash troughs supplied with this instrument have been
found suitable for use with most solutions and reagents used in staining routines.
6.1 INTRODUCTION
Correct service and maintenance is essential for the long term serviceability of
precision engineered products such as the Shandon Varistain Gemini stainer. Only a
qualified Thermo service engineer must set up and service the instrument, to ensure
future reliability and consistency of performance.
Table 1 shows remedial action to be taken if the Shandon Varistain Gemini stainer does
not operate correctly.
Poor staining may be due to:
Solutions containing iodine may be used in the troughs, although ageing is accelerated
and the troughs will need to be replaced at approximately six-monthly intervals.
5.1.4
Cleaning the Heater Station Troughs (where applicable)
Use an appropriate wax removing solvent to clean away all traces of wax from the
Heater Station troughs.
5.1.5
•
•
•
•
•
•
Varying fill levels - the slides are not fully covered by reagents during the staining
process
Wax is not completely removed from the specimen,
Inadequate fixation of the specimen
Inadequate dehydration of the specimen
Inadequate clearing of the specimen
Inadequate impregnation of the specimen with paraffin wax
The following tables list some typical problems and their remedies that may occur
during Haematoxylin and Eosin (H&E) or Papanicolaou (PAP) staining:
Washing Procedures
It is recommended that the troughs, baskets and carriers are washed at a maximum
temperature of 45°C and that they are allowed to drain or hand dry.
Table 2 - NUCLEAR STAINING PROBLEMS
Table 3 - CYTOPLASMIC STAINING PROBLEMS
Table 4 - MISCELLANEOUS STAINING PROBLEMS.
WARNING Do not use a dishwasher.
Table 5 - TROUBLESHOOTING HAEMATOXYLIN STAINING
WARNING Do not use detergents that contain bleach.
5.1.5
Table 6 - TROUBLESHOOTING PAPANICOLAOU STAINING
Monitor
IF A WARNING SCREEN IS DISPLAYED AT ANY TIME, FOLLOW THE
INSTRUCTIONS ON THE SCREEN.2 If the cursor is in area (b) or (c), details of the
steps where the highlighted reagent is used in each procedure will be displayed (g).
THE MONITOR IS NOT RESISTANT TO SOLVENTS.
5.2 MAINTENANCE
The Shandon Varistain Gemini stainer requires annual serving by a qualified service
engineer.
64
A78010402 Issue 4
In the example shown on page 43, 100% alcohol in reagent troughs 9 and 15 are used
in step 2 of the H&E procedure, step 5 of the PAP and step 6 of the PAS procedure.
A78010402 Issue 4
65
TABLE 1 - EQUIPMENT STATUS CHECKS
SYMPTOM
1
Standby LED not lit on the
PSU
Arm does not move
(+ = H&E only; * = PAP only)
CAUSE
a
b
2
TABLE 2 - NUCLEAR STAINING PROBLEMS
Power Supply Unit not
connected
Power Supply Unit not
switched on
c
Mains fuse blown
d
Instrument fuse blown
REMEDY
a
SYMPTOM
Check the connections
b
Switch on PSU
c
d
Replace mains fuse
Replace the instrument
fuse
1
No nuclear staining
or staining too light
Note:
Only an authorised
technician should
replace fuses
a
Main door is open
a
Make sure that both main
doors are closed
b
Load / Unload door is
open
b
Make sure that the Load
and Unload doors are
closed
CAUSE
REMEDY
a
Haematoxylin exhausted
a
Replace with fresh
haematoxylin
b
Differentiation too strong
(over-differentiation)
b
Dilute differentiating solution
Remove, or decrease time
c
Chlorine in tap water
c
Use bluing reagent
Use distilled water
d
Staining time too short in
haematoxylin
d
Increase staining time
*e Cells air dried
e
Irretrievable. Fix promptly
f
Haematoxylin too weak
f
Choose the type of
haematoxylin with the correct
strength
g
Haematoxylin expired
g
Replace with fresh
haematoxylin
+h Autopsy specimen
h
Increase time in haematoxylin
i
Autolysis started
i
Irretrievable. Fix promptly
+j Prolonged specimen
storage in formalin
j
Increase time in haematoxylin
k
k
Check dye content and adjust
formula accordingly
Haematoxylin prepared
inaccurately
Check formula to assure that
correct dilution is done
l
Stain cold
m pH of haematoxylin
incorrect
66
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n
Incomplete hydration
o
Slides allowed to dry after
initial hydration
l
Use warmer stains. The colder
they are, the slower they work
m Adjust pH with glacial acetic
acid
n
Thoroughly hydrate slides prior
to immersion in haematoxylin
o
Do not let slides dry from
initial submersion through
coverslipping
67
TABLE 2 - (Continuation)
TABLE 2 - (Continuation)
(+ = H&E only; * = PAP only)
(+ = H&E only; * = PAP only)
SYMPTOM
SYMPTOM
CAUSE
REMEDY
+p Deparaffinisation incomplete
p
Return to xylene to complete
deparaffinisation
q
Immerse in saturated mercuric
chloride for 10 min.
+q Over decalcification
*r
2
Nuclear detail
lacking (smudged,
grey)
No “crisp”
chromatin, bland
appearance
Incomplete removal of
Carbowax
r
Soak slides in 95% alcohol for
10 min. prior to staining
Haematoxylin exhausted
a
Replace with fresh
haematoxylin
b
Chlorine in tap water
b
Use distilled water
*c Cells air dried
c
Irretrievable. Fix promptly
d
d
Adjust pH with glacial acetic
acid
e
f
Incomplete hydration
Slides allowed to air dry after
initial hydration
e
f
3
Do not let slides dry from
initial submersion through
coverslipping
g
Return to xylene to complete
deparaffinisation
h
Immerse in saturated mercuric
chloride for 10 minutes
i
Soak slides in 95% alcohol for
10 min. prior to staining
j
Irretrievable. Fix promptly
k
Check pH pf bluing reagent
and change if necessary
+h Overdecalcification
Incomplete removal of
carbowax
j
Autolysis started
k
Bluing step ineffective
+l Prolonged specimen storage
in formalin
68
Increase time in bluing reagent
l
Nuclear staining too
dark
Thoroughly hydrate slides
prior to immersion in
haematoxylin
4
+g Deparaffinisation incomplete
*i
REMEDY
m Wash specimens more
thoroughly
+n Burned tissue
a
pH of haematoxylin too high
CAUSE
+m Burned tissue
Nuclei reddish/
purple
o
Haematoxylin expired
p
Washes after
haematoxylin, acid
rinse and bluing steps
exhausted
a
Haematoxylin time too
long
b
Haematoxylin too strong
c
Differentiating time too
short
d
Differentiating solution too
weak
a
Bluing ineffective
n
Irretrievable.
o
Change haematoxylin
p
Use running water rinses, or
change more frequently
a
Reduce time in haematoxylin
b
Dilute haematoxylin
Change to Gill or Mayer’s
c
Increase time
d
Use stronger (more acidic)
solution
a
Increase time in bluing reagent
Check pH of bluing reagent aid
change if necessary
b
Nuclei taking up eosin
b
Increase time in differentiating
step after counterstain
Lower the pH of the eosin
Change alcohols after
counterstain
5
Brown nuclei
a
Over oxidised
haematoxylin
a
Use fresh haematoxylin
Mordant in Zenker’s or Bouin’s
before staining
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69
TABLE 2 - (Continuation)
TABLE 3 - CYTOPLASMIC STAINING PROBLEMS
(+ = H&E only; * = PAP only)
SYMPTOM
6
7
Nucleoli taking up
haematoxylin
Artificially enlarged
nucleoli
(+ = H&E only; * = PAP only)
CAUSE
a
Haematoxylin too
concentrated
REMEDY
a
Dilute haematoxylin
SYMPTOM
1
Counterstain too dark
CAUSE
a
Stain too strong
REMEDY
a
Dilute stain (use appropriate
solvent)
Check percentage dye content
and adjust formula if necessary
b
Staining time too long
b
Decrease time in haematoxylin
c
Differentiating solution too
weak
c
Change solution
b
Stain diluted with
improper solvent
b
Use correct solvent
d
Differentiating step too
short
d
Increase time
c
Staining time too long
c
Decrease time
d
Differentiating time too
short
d
Increase time
e
Differentiating solution
exhausted
e
Use fresh solution
f
Differentiating solution
not strong enough
f
Use different solvent
g
Stain warm, penetrates
faster
g
Adjust time accordingly
+a Specimen in fixative too
long
a
Increase staining time
+-b Autopsy specimen
b
Increase staining time
c
c
Do not dilute if intended for full
strength use
a
Air drying
a
Irretrievable. Fix promptly
2
Counterstain too light
Stain too weak
Increase time in stain
Check percentage dye content
and adjust if necessary
If exhausted, replace with fresh
stain
d
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pH of stain incorrect
d
Adjust pH with glacial acetic
acid
71
TABLE 3 - (Continuation)
TABLE 3 - (Continuation)
(+ = H&E only; * = PAP only)
SYMPTOM
(+ = H&E only; * = PAP only)
CAUSE
e
Differentiation too
strong
REMEDY
e
Dilute differentiating solution
SYMPTOM
CAUSE
REMEDY
6
a
Poor levelling
a
Increase time in counterstain
b
Solutions evaporated
b
All solutions should cover
specimen Replenish
c
Change xylene
Uneven cytoplasmic
staining
Use different solvent
Decrease time
+c Incomplete
deparaffinisation
f
Expired stain
f
Use fresh stain
g
Stain diluted with
improper solvent
g
Use correct solvent
h
Stain cold
h
Increase staining time
a
High RNA content
a
Increase differentiation time
Increase time in clearing agent
b
pH of haematoxylin too
high
b
Readjust pH with glacial acetic
acid
c
Haematoxylin in too
strong
c
Decrease time in haematoxylin
Level of xylene should exceed
previous absolute alcohol which
in turn should exceed previous
95%
a
Not enough light green
a
Use EA-50 instead of EA-65
b
Improper fixation
b
Irretrievable
c
Inflammation
c
Expected finding
d
Inadequate
differentiation after EA
step
d
Increase time in alcohol
Use fresh solution
a
Poor levelling
a
b
pH of EA incorrect
c
d
Deparaffinize longer
7
3
Cytoplasm is taking
up haematoxylin
4 * Pseudoeosinophilia
5* Amphophilia
72
Poor cytoplasmic
transparency (cells
appear dull, murky)
d
Inadequate time
d
Increase time in counterstain
a
Improper clearing
a
Change absolute alcohol &
xylene frequently to prevent
contamination with water
+
Xylene used for deparaffinisation
should not be used to clear
b
Too much mounting
media used
b
Use minimal amount
c
Microscope not cleaned
/ aligned
c
Clean microscope and use
Kohler illumination
Increase time in EA
d
Stain expired
d
Change counterstain
b
Check pH and adjust if
necessary
e
Stain exhausted
e
Change counterstain
EA expired
c
Use fresh EA
f
Incomplete
deparaffinisation
f
Deparaffinize completely
EA exhausted
d
Use fresh EA
*g Incomplete removal of
carbowax
g
Soak slides for 10 min. in 95%
alcohol prior to staining
h
h
Irretrievable
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Poor fixation
73
TABLE 4 - MISCELLANEOUS STAINING PROBLEMS
TABLE 3 - (Continuation)
(+ = H&E only; * = PAP only
(+ = H&E only; * = PAP only)
SYMPTOM
8* No OG uptake
CAUSE
10 Counterstain in
nuclei
CAUSE
a
Appears that albumin/
gelatin adhesive stained
by haematoxylin or
haematoxylin affinity for
glass
REMEDY
a
Add acid rinse step
i
Change alcohol rinses following
counterstains frequently
j
Inadequate dehydration
j
Dehydrate properly before
counterstaining
a
No keratinized cells
present
a
None - expected finding
Increase strength of acid solution
(increase acid, decrease alcohol
content)
b
pH of OG-6 too high
b
Adjust pH with glacial acetic acid
Changed differentiating solution
c
Change stain
d
Change stain
e
Use 95% ethanol
f
Increase time
g
Decrease time
d
Brown artifact (corn
flakes)
1+ Blue haze
surrounding
sections
Differentiating solutions
exhausted
c
9
SYMPTOM
REMEDY
i
2
OG-6 exhausted
Blue/black
precipitate seen
a
Haematoxylin has
precipitated
Increase time in acid
differentiation step
a
If precipitate will not filter out
(Gill’s haematoxylin) change and
use fresh
OG-6 expired
e
Solvent used to
differentiate too strong
f
Staining time too short
Filter haematoxylin
3+ Formalin pigment
g
Differentiation time too
long
a
Xylene drying
a
Promptly coverslip slides after
removal from xylene
a
Nuclear staining not
adequate
a
Increase time in haematoxylin
a
Tissue fixed in
nonbuffered formalin
a
To remove pigment, immerse
sections in saturated picric acid in
95% alcohol
To prevent pigment, use buffered
formalin
4+ Sections yellow
a
Tissue stored in Bouin’s
fixative (Picric acid)
a
Place sections in a filtered
saturated alcoholic solution of
lithium carbonate until yellow
is removed and proceed with
hydration
5+ Sections falling off
slide
a
Not “baked” thoroughly
a
Increase time/temperature
b
No adhesive used
b
Add gelatin to waterbath or use
albuminized slides
c
Dirty slides
c
Clean slides
d
Bluing reagent too harsh
d
Use gentle bluing reagent
Increase strength of hematoxylin
Decrease time in differentiating
step
Increase strength of
differentiating step
b
b
Cytoplasmic stain not
differentiated completely
Change differentiating solutions,
use fresh
Increase time in differentiating
solution
Use stronger solvent for
differentiation
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75
TABLE 4 - (Continuation)
TABLE 4 - (Continuation)
(+ = H&E only; * = PAP only)
(+ = H&E only; * = PAP only)
SYMPTOM
CAUSE
REMEDY
6
Floaters
a
Stains and reagents
contain dislodged cells
a
Change or filter solution
7
Halos seen
a
Microscope not aligned
a
Use Kohler illumination
b
Mounting medium has
incorrect refractive index
b
Change mounting medium
8* Shrunken cells
a
Inappropriate fixative
used
a
Use correct fixative
9
a
Contaminated with water
a
Change xylene and absolute
alcohols using clean dry dishes
b
Change absolute alcohols
more frequently
Xylene is cloudy
(milky)
10 Water droplets seen
a
Contaminated clearing
agent or mounting
medium
a
Use fresh solutions
11 Air bubbles
a
Air trapped during
coverslipping
a
If they can’t be “teased” out,
remove coverslip and reapply,
using minimal amount of
mounting medium
SYMPTOM
CAUSE
REMEDY
13+Clear patches
in sections after
immersion in absolute
alcohol, following
deparaffinisation
a
Deparaffinisation is
incomplete
a
Return slides to xylene and
hydrate as usual
14 Slides appear
opaque or cloudy
after clearing
a
Inadequate time in
dehydrating solution
a
Increase time
b
Dehydrating solution
contains water
b
Use fresh solutions
a
Water is trapped under
paraffin, slide not dried &
deparaffinized properly
a
Place slides in absolute
alcohol for several minutes,
return to xylene to complete
deparaffinisation and hydrate
as usual
15+ White patches
in sections after
hydration
Use thinner mounting medium
12 At completion of
hydration, slides appear
“bubbly”
76
a
Hydration is incomplete
a
Return slides to water until
runoff is smooth
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TABLE 5 - TROUBLESHOOTING HAEMATOXYLIN STAINING
TABLE 6 - TROUBLESHOOTING PAPANICOLAOU STAINING
SYMPTOM
SYMPTOM
1
2
3
4
No Stain
Grey
Purple
Red to brown
Resources -
REMEDY
a
Exhausted haematoxylin
b
Excessive chlorine in tap water used for
bluing or rinsing
a
Over-oxidation
b
Over-processed specimen
a
Acidic Tap Water
b
Too little time in bluing
c
REMEDY
1
Red nuclei
a
Increase time in bluing reagent
2
Nuclear staining too weak
a
Increase staining time
b
Use higher strength haematoxylin
c
Reduce concentration (not time) of acid
alcohol
3
Background haematoxylin staining
a
Increase time in acid alcohol
4
Overall colour too blue
a
Increase time in acid alcohol
Exhausted bluing solution
5
Cytoplasm too pink
a
Increase time in OG-6
a
Badly over-oxidised
6
Cytoplasm too orange
a
Increase time in alcohol after OG-6
b
Long term exposure to air or sunlight
7
Cytoplasm too blue
a
Increase time in EA-50
8
Cytoplasm too green
a
Increase time in alcohol after EA-50
Thermo staff and Luna, L; Histologic Methods and Color Atlas
of Special Stains and Tissue Artifacts, p. 70. 1992. Copyright
American Histolabs Inc.
It is important to remember that a number of minor modifications may be required to
achieve the final desired result.
Alteration to the procedure should be done in a step-by-step process towards the
desired goal. Start with the major problem and then build on that. For high quality
results, it is important to ensure that all haematoxylin is removed from the cytoplasm.
Therefore, a long treatment in acid alcohol is beneficial.
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SPECIFICATION AND ACCESSORIES
7.1.4
7.1 SPECIFICATION
Power On
Power Off
7.1.1
Switch Convention
I
O
Physical
Stainer Unit
Width
Depth
Height
Weight
720mm (281⁄2 ins)
710mm (28 ins)
880mm (341⁄2 ins)
80kg (176lbs)
7.1.5
Water Supply
Minimum
Maximum
0.2 millibars (3psi)
10.0 millibars (145psi)
If water pressure is more than 10 millibars, install a pressure-reducing valve.
Power Supply Unit
Monitor
Width
Depth
Height
Weight
Refer to manufacturer’s data
Electrical
Power Supply Voltages
Frequency
Power
Monitor Voltage
Frequency
7.1.3
7.1.6
Standard PC compatible colour monitor
Weight
7.1.2
360mm (14 ins)
463mm (181⁄4 ins)
145mm (61⁄2 ins)
11kg (24lbs)
100 - 240Vac (~)5A
50/60 Hz
150VA (non-heated version)/
300VA (heated version)
General
Temperature
(operation)
Indoor use only
+5°C to +40°C
Temperature
(transit/storage)
-25°C to +55°C
Humidity
80% max. for temperatures < 31°C
50% max. for temperatures 31°C to 40°C
(Non-condensing environment)
7.1.7
100 - 240Vac (~)3A
50/60 Hz
Fuses
Environment
Shandon Varistain Gemini Automatic Stainer Part Numbers
No Heat
Dual Voltage
No Monitor
With Monitor
A78000001
A78000002
With Heat
Dual Voltage
No Monitor
With monitor
A78000011
A78000012
Thermo Part Number
Mains plug fuse
Mains input fuses
x2
Non-heated
Heated
13A 250V
T5A
T10A
A78010465
FUSES SHOULD ONLY BE REPLACED BY TECHNICALLY COMPETENT
PERSONNEL.
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7.2 ACCESSORIES
THERMO PART NUMBER
Baskets/Carriers (pack of 5) (each holds 20 slides)
A78010466
Basket Adaptor for large slides (pack of 10)
Slide size - up to 75 x 58mm (3” x 21⁄4”)
A78010510
Part 1
Carrier for Sakura basket (pack of 5)
A78010404
Any instrument or part of any instrument must be clean before being returned, and where necessary
accompanied by a completed Decontamination Certificate. Should the instrument or any part of it be
received in an unclean condition, or Thermo Electron Corporation consider it to be a hazard, the instrument
or part will be returned unrepaired at the expense of the customer.
Consul Clip Transfer Tool
A78010401
PRODUCT RETURN SAFETY DECLARATION
DECONTAMINATION CERTIFICATE
It is important that the certificate is forwarded by post or fax, and a copy attached to the exterior of the
container. Containers will not be opened until the company is in possession of the required certificate.
This form MUST be completed by the customer and NOT a Thermo or distributor employee.
Consul Clip Rod and extension kit
A74110291
If an instrument or part is to be returned to THERMO, please note the following:-
Charcoal Filter
9990610
1 If the instrument or any part of it has been exposed to, or been in contact with potential pathogenic or
radioactive material, it is essential that it is decontaminated.
Reagent Troughs (pack of 3)
(Each trough volume = 320ml)
A78010487
2 Set procedures are laid down in the European Health and Safety Directives for decontamination. To avoid
any misunderstanding, we request that all instruments or parts returned to us must be accompanied by a
certificate stating the following:
We certify that this (Model) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Serial No
· has not been exposed to pathogenic, radioactive or other hazardous material and has been cleaned
Reagent Trough covers - large (pack of 4)
A78010488
OR
Reagent Trough Covers - single trough (pack of 6)
A78010489
· has been decontaminated and cleaned (if exposed to the above) according to approved procedures,
following exposure to:
Vent Kit
A78010467
VGA Monitor
AP14172
VGA Extension Cable
AP14185
Signed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Water Trough
A78010201
Company or Organisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Water Wash Inlet Hose
AP14160
...................................................................................
· Has the instrument been used for work with human, or animal, Transmissible Spongiform
Encephalopathies, e.g. Creutzfeld-Jacob disease, Scrapie or BSE?
YES / NO
If yes, please contact Thermo Service before taking any further action.
Position . . . . . . . . . . . . . . . . . . . . . . . . .
Name (Block Capitals) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Full address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
.....................................................................................
Part 2 Guidelines for Returning Instruments
Please use the checklist below to ensure that the instrument being returned is ready for collection.
· All reagents / wax removed from instrument, including vapour traps (if applicable). . . . . . . . . . . . . . . . .
· Accessories are secured / itemised . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
· Instrument has had transit clamps fitted as per operator guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
· Instrument is packed in original packaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . YES / NO
RMA NUMBER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
CARRIER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
FOR ATTENTION OF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Thermo Electron Corporation, 93-96 Chadwick Road, Astmoor, Runcorn, Cheshire, WA7 1PR, United Kingdom
Tel: +44 (0) 1928 566611; Fax: +44 (0) 1928 565845; www.thermo.com/shandon
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Declaration of Conformity
WARRANTY STATEMENT
This Declaration of Conformity is only valid when the instrument is used in accordance with this Operator Manual
Thermo is proud of its quality and reliability, and of its after-sales service. Thermo
continuously strives to improve its service to its customers.
Ask your distributor or representative about Service Contracts, which can keep your
purchase in peak condition for many years to come.
Manufacturer’s Name:
Manufacturer’s Address:
Thermo Electron Corporation
Chadwick Road, Astmoor, Runcorn,
Cheshire, WA7 1PR
ENGLAND
Product Description: Automatic Stainer (heated or non-heated)
Product Designation: Shandon Varistain Gemini
(A78000001, A78000002,
A78000011, A78000012)
including accessories supplied as standard
Warranty provisions necessarily vary to comply with differences in national and regional
legislation, and you can find details in your delivery documents, or from your dealer or
representative.
Year of Marking (CE):
2000
This product conforms with the essential requirements of the following directives:
In Vitro Diagnostics Directive 98/79/EC
Low Voltage Directive 73/23/EEC (as amended by 93/68/EEC)
Note that your warranty may be invalidated if:
This product complies with the following International Standards:
EMC:
•
The instrument is modified in any way
•
Accessories and reagents are used that are not approved by Thermo
•
The instrument is not operated or maintained in accordance with the instructions
in this Operator Guide
Safety:
EN61326
EN61000-3-2
EN61000-3-3
IEC1010-1 - non-heated version
IEC1010-2-010 - heated version only
CAN / CSA - C22.2 No 1010.1-92
UL Std No. 3101
Issued by:
K Waldron
Quality Manager
Thermo Electron Corporation
Anatomical Pathology, Clinical Diagnostics
Date: 23 September 2003
Optional accessories considered subject to the In Vitro Diagnostic Directive (IVDD) are specifically identified on
this Declaration of Conformity. Further supplies of standard accessories are treated as spares. Convenience aids
offered as accessories are not subject to the IVDD
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APPENDIX A TRANSPORTATION
INSTRUCTIONS
a
Repackage the monitor in accordance with the
manufacturer’s instructions.
A.1 INTRODUCTION
If you ever need to transport the Shandon Varistain Gemini stainer, the following
packaging instructions should be followed.
Empty and dry the water wash troughs. Empty
out as much water as possible from the black
drain hose. Coil the hose at the rear of the
instrument and use cable ties at the two fixing
points (a) to fix the coil to the back panel of the
instrument.
A.2 TO REPACKAGE THE STAINER UNIT
Note
1
b
l
Make sure that all the baskets have been
removed from the instrument and that the
hook is in the left hand hole in the upper
deck (as viewed from the front of the
instrument)
a
0
If the Shandon Varistain Gemini
stainer is to be serviced or returned
to Thermo, it must be cleaned and
decontaminated, and the Product
Return Safety Declaration (see page
83) signed.
Turn off the water supply and disconnect the
blue inlet hose at the right hand rear corner.
Place the inlet hose (AP14160) and Jubilee clip
(P12026) into a bag.
Gently press on the hook to lower it by
approximately 25 - 50mm. Carefully fit the Hook
Support foam piece A to the hook from the top.
This grips the hook and top deck to prevent any
movement of the mechanism.
A
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Product:
Cat No:
Serial No:
Nominal Ratings:
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A78010351 Issue 10
PS0001E0001
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Place a basket and carrier into pots 4, 5, 6, 15,
16, 17 on the lower deck and W2, W3, W4 and
W5 on the upper deck (10 in total).
c
Press the O (OFF) side of the mains power
switch (a) to switch the mains power switch
off.
Place the four multi-trough lids (A78030215)
in two stacks on top of the lower level of
troughs.
Wait for about 30 seconds while the
instrument shuts down, and then press the
O (OFF) side of the Battery Isolation Switch (b).
Disconnect the mains cable from the mains supply and the Power Supply Unit.
D
Place the two semi-circular cardboard
pieces D over the pots - four on each level,
overlapped by 90° as shown.
Disconnect all cables between the Power Supply Unit, the Shandon Varistain Gemini
stainer and the monitor (c).
86
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87
Place the two foam pieces B between the
cardboard on the top deck and the top cover.
These should be pushed into place to keep
the cardboard pieces D in position.
Place the two shorter foam pieces C between
the cardboard on the lower deck and the
underside of the top deck at the water wash
positions. Again, these should be pushed
into place to keep the cardboard pieces D in
position.
Place the bag containing the inlet hose at the rear of the instrument. Cling film the
outside of the unit using packaging film - three full circuits around the unit starting at the
top front and finishing at the bottom.
B
D
Gently push the foam piece E between the
upper sliding door and upper layer of pots.
Gently push the smaller foam piece F between
the lower sliding door and the turntable.
THE SHANDON VARISTAIN GEMINI
STAINER IS HEAVY, GET HELP TO LIFT IT.
HOLD THE STAINER SECURELY AT THE
LIFTING POSITIONS LOCATED UNDER
THE SIDES OF THE INSTRUMENT (b).
C
b
b
Place the stainer onto the skid base so that the
unit sits in the foam perimeter (c).
E
Note
1
F
The front of the instrument is indicated
on the skid base.
e
Place the foam over-shipper over the unit (d).
Place three spare troughs into a bag. (Place them
in line as shown in the diagram (a)).
Place the cardboard over-shipper over the foam
over-shipper (e).
Place six small trough lids (A78030142) into two
polythene bags (three in each), and place in the
bag with the troughs.
a
d
Seal and staple box and place two bands
around to secure the box to the skid. The
stainer is now ready to be transported.
c
Tie wrap the bag containing the three troughs and trough lids to the lower cardboard ‘C’
piece inside the unit.
Close the large doors and ensure the sliding doors are in the central position.
88
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APPENDIX B APPROVED REAGENT
LIST
A.3 TO REPACKAGE THE POWER SUPPLY UNIT
Get help to lift the Power Supply Unit if necessary.
Hold the unit securely at the lifting positions
located under the sides of the unit (a).
B.1 INTRODUCTION
a
Refer to ‘Safety and Chemicals’ section on page 7 before the instrument is
WARNING filled with reagents.
b
The Battery Isolation Switch (b) must be switched
to the OFF position (O on Battery Isolation Switch
pushed inward).
If you want to use a reagent not included in this list, contact your Thermo agent for
advice.
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��
���������� ����������
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�������� ��������
Product:
Cat No:
Serial No:
Nominal Ratings:
�
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A78010351 Issue 10
PS0001E0001
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c
Fit the two side foam pieces
(c) to either side of the
power supply and carefully
place in the packing box (d).
B.2 REAGENTS
d
Place the manual in a polythene bag on top of PSU.
e
This Section lists all the reagents that Thermo specify can be used with the Shandon
Varistain Gemini stainer.
Put each mains lead into a polythene bag (six
mains leads - 2 x UK (AP14202); 2 x USA
(P12032); 2 x Europe (P12822)). Place these into
the spaces in the PSU pack (e).
Put the data lead (A78030174) and monitor
extension lead (AP14185) separate polythene
bags. Place these into the spaces in the
PSU pack (e).
Xylene
Toluene
Shandon Xylene Substitute
Industrial Methylated Spirits (IMS)
Ethanol
Isopropanol (IPA)
Acid Alcohol
Acid Alcohol
Eosin
Haematoxylin
EA50/65
OG6
Phosphotungstic acid
Ammonia Water
Scotts Bluing Reagent
Sodium Hypochlorite
Water
Schiff’s Reagent
Periodic Acid
(Shandon Histosolve)
(up to 5% methanol in ethanol)
(1% HCI in 70% Ethanol)
(10% Glacial Acetic Acid in 70% Ethanol)
(Aqueous and Alcohol based)
(1% in water)
(1% Ammonium Hydroxide in water)
(10% in water)
Close, staple and tape box. The Power Supply Unit is now ready to be transported.
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APPENDIX C EXAMPLE STAINING
PROCEDURES
C.1 INTRODUCTION
These staining protocols are provided for convenience only - you may need to modify
them to suit your reagents, water and other local conditions. Thermo has not validated
the protocols illustrated here, and takes no responsibility for their use. Customers
should always validate protocols before placing any reliance on them.
PRE-STAIN
C.3 PAPANICOLAOU (PAP) STAINING ROUTINE
POST-STAIN
92
TIME (mins:secs)
CONC. %
TIME (mins:secs)
1
Alcohol (IMS)
95.0
1:00
2
Alcohol (IMS)
95.0
1:00
3
Alcohol (IMS)
70.0
1:00
4
Water Wash
1:00
5
Haematoxylin
4:00
6
Water Wash
1:00
7
Acid Alcohol
0:15
8
Water Wash
1:00
9
Bluing Reagent
1:00
10
Water Wash
1:00
11
Alcohol (IMS)
70.0
1:00
12
Alcohol (IMS)
95.0
1:00
13
OG6
REAGENT
1
Xylene
2:00
2
Xylene
1:00
3
Xylene
1:00
4
Alcohol (IMS)
100.0
1:00
5
Alcohol (IMS)
100.0
1:00
6
Alcohol (IMS)
100.0
1:00
14
Alcohol (IMS)
95.0
1:00
7
Water Wash
1:00
15
Alcohol (IMS)
95.0
1:00
8
Haematoxylin
4:00
16
EA50
9
Water Wash
1:00
17
Alcohol (IMS)
95.0
1:00
10
Acid Alcohol
0:15
18
Alcohol (IMS)
100.0
1:00
11
Water Wash
1:00
19
Alcohol (IMS)
100.0
1:00
12
Bluing Reagent
1:00
13
Water Wash
1:00
20
Alcohol (IMS)
100.0
1:00
14
Aqueous Eosin
1:00
21
Xylene
1:00
15
Water Wash
1:00
22
Xylene
1:00
16
Alcohol (IMS)
100.0
1:00
23
Xylene
1:00
17
Alcohol (IMS)
100.0
1:00
18
Alcohol (IMS)
100.0
1:00
19
Xylene
1:00
20
Xylene
1:00
21
Xylene
1:00
STAIN
CONC. %
REAGENT
STEP
POST-STAIN
STAIN
PRE-STAIN
C.2 HAEMATOXYLIN AND EOSIN STAINING ROUTINE
STEP
A78010402 Issue 4
A78010402 Issue 4
2:00
3:00
93
POST-STAIN
STAIN
PRE-STAIN
C.4 PERIODIC ACID SCHIFF (PAS) STAINING ROUTINE
94
STEP
REAGENT
CONC. %
TIME (mins:secs)
1
Xylene
2:00
2
Xylene
1:00
3
Xylene
1:00
4
Alcohol (IMS)
100.0
1:00
5
Alcohol (IMS)
100.0
1:00
6
Alcohol (IMS)
100.0
1:00
7
Water Wash
1:00
8
Periodic Acid
5:00
9
Water Wash
1:00
10
Schiff’s Reagent
5:00
11
Water Wash
5:00
12
Haematoxylin
2:00
13
Water Wash
1:00
14
Bluing Reagent
1:00
15
Water Wash
1:00
16
Alcohol (IMS)
100.0
1:00
17
Alcohol (IMS)
100.0
1:00
18
Alcohol (IMS)
100.0
1:00
19
Xylene
1:00
20
Xylene
1:00
21
Xylene
1:00
A78010402 Issue 4
APPENDIX D TO PERFORM A MEMORY
RESET
D.1 INTRODUCTION
Follow these instructions to perform a memory reset if the sequence for turning the
instrument off has not been carried out correctly.
ALL OPERATIONAL STATUS INFORMATION WILL BE LOST.
D.2 INSTRUCTIONS
1
Switch the instrument on by following the instructions in Section 2.9.
2
When the language select screen is displayed, press [
3
When the Help screen is displayed, press [7].
4
When the Help screen disappears, a full memory reset will have been carried
out.
5
Reset the clock (see paragraph 4.3.1).
6
Reload the instrument setup from disk (paragraph 4.3.5), or follow the instructions
in Section 4.2 to program the instrument.
A78010402 Issue 4
].
95
INDEX
A
Accessory Instructions
Consul® Clip Transfer Tool 21
Vent Kit 23
Access Codes
To change an access code 61
To clear an access code 62
To set an access code 61
To use the access code 62
APPROVED REAGENT LIST 91
Introduction 91
Reagents 91
Audio Configuration Screen
[CONTINUOUS] 59
[DEMONSTRATE] 59
[NEW TUNE] 59
[SILENCE] 59
B
Batch Monitoring Screen 52
[AUTO RETURN] 54
[STOP] 54
Battery Charging 20
Battery Disposal 6
Battery Isolation Switch 19, 86, 90
Battery Type 7
C
CLEANING AND MAINTENANCE
Cleaning
Cleaning the Power Supply Unit 64
Cleaning the Stainer 63
Washing Procedures 64
Maintenance 64
Connecting the system 16
To Connect a Monitor to the Stainer 16
To Connect a Printer 17
Recommended Printer Types 17
Consul® Clip Transfer Tool 10, 21
CONTROLS 25
Description 25
Main Control Panel 25
[*] 27
[CANCEL] 27
[ENTER] 26
[EXIT] 27
Direction Arrow Keys 27
Function Buttons 25
Help Key 27
96
On/Off Switch 18
Wiring Convention 18
Procedure 11
Overall Dimensions - PSU 12
Overall Dimensions - Stainer 11
Siting Requirements 11
Water Supply Position 11
To Adjust the Turntable 15
To Connect the System 16
To Connect a Monitor 16
To Connect a Printer
Recommended Printer Types 17
Number Keys 26
Silence Alarm 27
Standby Key 27
D
DATA MANAGEMENT 58, 60, 61
[DISC DRIVE] 60, 61
[PRINTOUTS] 60, 61
Declaration of Conformity 85
Define Procedure Screen 35
[CHANGE REAGENT] 36
[DELETE STEP] 35
[INSERT STEP] 35
Define Reagent Names 32
DESCRIPTION 9
Overview 9
Disc Drive Options Screen 61
[START] 61
E
Earthing Requirements 18
Electrical Requirements 18
EXAMPLE STAINING PROCEDURES 92
HAEMATOXYLIN AND EOSIN STAINING
ROUTINE 92
INTRODUCTION 92
PAPANICOLAOU (PAP) STAINING ROUTINE 93
PERIODIC ACID SCHIFF (PAS) STAINING
ROUTINE 94
F
Filter, To Fit 14
H
Heater Station Troughs - to remove and replace
31
How to Stain 31, 39, 49
I
INSTALLATION AND SETTING UP 11
Accessories 21
Consul® Clip Transfer Tool 21
Large Slide Basket Adaptor 24
Vent Kit 23
Electrical Requirements 18
Battery Isolation Switch 19, 86, 90
A78010402 Issue 4
To Fit the Filter 14
To Level the Instrument 13
To Adjust the Levelling Feet 13
To Switch on and off 19
To Switch On 20
To Unpack 12
Lifting the Power Supply Unit 12
Lifting the Stainer 12, 13
Positioning the Monitor 13
On / Off 59
On / Off Switch 59
OPERATION 29
Housekeeping Tasks 58
Quality Control Settings 58
To Change the Time and Date 58
To Save or Retrieve Information From a
Floppy Disk 60
How to Stain 29
Introduction 29
Define the Reagents to be Used 30
Define the Staining Procedures to be Used
32
Quality Control Warning Screen 57
Reagent Layout 1 Screen 39
Reagent Layout 2 Screen 42
Heater Sharing Control Screen 47
Reagent Sharing Control Screen 46
Water Wash Sharing Control Screen 47
Select the Stain Program 30
K
P
keyboard 31, 34
Keyboard Screens 31
[CAPS LOCK] 32
[COMPLETE] 32
[CONTROL] 32
Printer 60
Printout Options Screen 60
[DISC DRIVE] 60
[ON-SCREEN] 60
[ PRINTER] 60
Procedure Select Screen 33
[CHANGE NAME] 34
[CHANGE NOTES] 34
[REMOVE] 33
[RETAIN] 33
[SELECT] 33
Product Return Safety Declaration 83
L
language 20, 30, 95
Language, To Select 20
lifting position 89, 90
Lifting Positions 89
loading of reagents 15, 51
Loading Reagents 51
Loading Slides Screen 55
[SET AS DEFAULT] 56
[STANDARD START] 55
[URGENT START] 55
Q
Quality Control Screen 59
[ON / OFF] 59
Quality Control Warning Screen 51, 53, 57
M
R
Main Menu Screen 26, 29, 30, 37, 58, 60, 61
[MONITORING] 26
[OPTIONS] 26
[PROGRAM] 26
[STAIN] 26
Material Safety Data Sheets (MSDSs) 2
Memory Reset 19
memory reset 19, 95
Reagent Layout 1 Screen 33, 39, 42, 45, 47,
56
[ADD PROCEDURE] 39
[DELETE PROCEDURE] 39
[ REAGENT LAYOUT 2] 39
Reagent Layout 2 Screen 39, 42
[ADD PROCEDURE] 45
[CHANGE GROUPS] 45
[CHANGE OPTIONS] 47
[COMPLETE] 47
O
A78010402 Issue 4
97
[DELETE] 47
[DUPLICATE] 48
[MOVE] 48
[OPTIMISE] 48
[AUTO ALLOCATE] 48
[AUTO ARRANGE] 48
[PRINT] 48
Reagent List 91
Reagent Monitoring Screen 49
[BATCH MONITORING] 52
[CONFIRM LOADED] 51
S
V
Vent Kit 14, 23
W
warnings 51, 53, 57, 58
WARRANTY STATEMENT 84
Water Supply and Drainage 14
WELCOME 5
Introduction 5
Safety 5
Safety and Chemicals 7
Safety and Chemicals 7, 49, 91
Setting Up 11
Set Clock 58
[12 / 24 Hour Clock] 58
[DAY <-> MONTH] 58
SPECIFICATION AND ACCESSORIES 80
Specification 80
Electrical 80
Fuses 80
Physical 80
Switch Convention 80
Water Supply 80
Staining 8, 9, 10, 26, 28, 29, 31, 32, 35, 39,
43, 44, 49, 50, 51, 53, 54, 57, 58, 59, 64,
65, 67, 68, 69, 71, 72, 73, 74, 79
SYMBOLS
Biohazard 2
Harmful chemicals 2
High temperatures 2
Safe operation 2
Warning 2
Symbols and Conventions 2
T
TRANSPORTATION INSTRUCTIONS 86
Introduction 86
To Repackage the PSU 90
To Repackage the Stainer 86
TROUBLE SHOOTING 65
CYTOPLASMIC STAINING PROBLEMS
71, 72
EQUIPMENT STATUS CHECKS 66
Introduction 65
NUCLEAR STAINING PROBLEMS 67
U
Unload 28, 41, 42, 55
Unpacking 11
98
A78010402 Issue 4
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