NikonA1-AandA1-Bdifferences
Thereare4majordifferencesbetweentheNikonA1AandtheNikonA1B
NIKONA1A
NIKONA1B
Whenturningthesystemonoroff,thereare
Whenturningthesystemonoroff,thereis
fourindividualkeyswhichcontrolthefour
onekeythatcontrolsthefourlasers.Turnthe
separatelasers.Allmustbeturnedto“ON”or
keyto“ON”or“Standby.”
“Standby.”
NIKONA1A
Toopentheshutter,usetheshutterwheelon
theXCitelightsourcebox.
1click=25%power
2clicks=50%power
3clicks=75%power
4clicks=100%power
NIKONA1A
Tobeginanewsample,powerforall4lasers
shouldbesetto3%.
NIKONA1B
Toopentheshutter,usetheblackshutter
buttontotherightofthemicroscope(press
downgentlyonce.)
Tochangethe%power,turnthebigbutton
itself.
NIKONA1B
Tobeginanewsamples,powerfortheDAPI
andfarredlasershouldbesetto3%.Power
for488and561lasersshouldbesetto1%as
thesehavehighsensitivitydetectors.
3
3
3
1
3
1 NIKONA1A
10xisaDRYobjective.
20xisOIL(orwater,orglycerol)
40xand60xobjectivesareOIL.
NIKONA1B
10xand20xarebothDRYobjectives.
Theconsecutive40xand60xobjectivesare
OIL(blackringaroundthetopoftheobjective)
Thefollowing40xand60xobjectivesare
WATER(whiteringaroundthetopofthe
objective)
Whenusingeitherofthemicroscopesforthefirsttime,you’llneedtoenablethemousescroll
wheeltoadjustfocusandHV/offsetsettings.Todothis,goto“Devices”inthetoptab,then
“Enablemousejoystick.”