Installation and operation of the DP25 for TWAIN

INSTRUCTIONS
DP25
MICROSCOPE DIGITAL CAMERA
This instruction manual is for the OLYMPUS DP25 Microscope Digital Camera. To
ensure safety, obtain optimum performance and familiarize yourself fully with the use of
this camera, we recommend that you study this manual thoroughly before operating the
camera.
Retain this manual in an easily accessible place near a system for future reference.
⎯⎯This publication is printed on 100% recycled paper.⎯⎯
Any copyrights relating to this manual shall belong to OLYMPUS CORPORATION. We at
OLYMPUS CORPORATION have tried to make the information contained in this manual as
accurate and reliable as possible. Nevertheless, OLYMPUS CORPORATION disclaims any
warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this
manual, including without limitation the merchantability or fitness for any particular purpose.
OLYMPUS CORPORATION will from time to time revise the software described in this manual
and reserves the right to make such changes without obligation to notify the purchaser. In no event
shall OLYMPUS CORPORATION be liable for any indirect, special, incidental, or consequential
damages arising out of purchase or use of this manual or the information contained herein.
No part of this document may be reproduced or transmitted in any form or by any means,
electronic or mechanical, for any purpose, without the prior written permission of
OLYMPUS CORPORATION.
Microsoft Windows, Microsoft Word, Microsoft Excel and Microsoft Access are trademarks of
Microsoft Corporation which can be registered in various countries. Adobe and Acrobat are
trademarks of Adobe Sytems Incorporated which can be registered in various countries.
© OLYMPUS CORPORATION
All rights reserved
Printed in Germany
Contents
Important information....................................................... 3
About the contents of this manual ..................................................................3
Warning ............................................................................................................3
Maintenance and Storage ................................................................................4
Conformity of the System ................................................................................4
Restrictions .................................................................................................................4
Operating Environment ...............................................................................................5
System Chart..................................................................... 6
Nomenclature .................................................................... 7
Installation and operation of the DP25 for TWAIN......... 8
System Requirements ......................................................................................8
Installation of the camera ................................................................................9
Using the TWAIN Interface .............................................................................20
Acquiring images ......................................................................................................21
Adjusting the exposure time .....................................................................................21
Carrying out a white balance ....................................................................................24
Selecting the resolution ............................................................................................24
Trimming the image (subarray) .................................................................................25
Focusing the sample .................................................................................................25
Zooming the image ...................................................................................................26
Shift Metering Regions .............................................................................................26
Settings .....................................................................................................................26
Installation of the DP25 for the DP2-BSW software..... 35
System Requirements ....................................................................................35
Installation of the camera ..............................................................................36
Acquiring your first images ...........................................................................47
Special camera functions ..............................................................................48
Contents
Specifications.................................................................. 50
Help with Problems......................................................... 52
Your image doesn't look as good as it should ..........................................................52
"Live" and "Snap" are not active ...............................................................................54
When connecting the camera to the Firewire interface before installing the DP25
TWAIN driver software or the DP2-BSW software ....................................................54
Declarations of Conformity............................................ 61
Important information
Important information
About the contents of this manual
The DP25 is a high resolution CCD color camera for large scale applications in the
brightfield microscopy field. Impressive features of this camera are its superior color
quality and rendition. Not to mention the DP25 camera's compact design and ease
of use.
The DP25 is a camera that you can use both with the DP2-BSW software of
OLYMPUS, as well as with any other software you wish, that supports TWAIN.
This manual is made up of two parts. Should you work with the DP2-BSW software,
you will only need to read the part "Installation of the DP25 for the DP2-BSW software", beginning on page 35.
Should you wish to use the camera without the DP2-BSW software, read only the
part "Installation and operation of the DP25 for TWAIN", beginning on page 8.
Warning
Please install the DP 25 TWAIN driver software (TWAIN Interface) or the software
with DP25 camera driver (especially DP2-BSW) before connecting the camera to the
FireWire interface.
Warning
Do not disassemble the camera housing! CCD image sensors are easily damaged
by static discharge.
Warning
To avoid damage to the cable and the risk of fire, make sure that the camera cables
do not come into contact with the light source or other sources of heat.
Warning
In case of a fire toxic gases can be given off!
Warning
Arrange the cables in such a way that nobody can stumble or trip over them.
Warning
Do not touch cover slip (over the CCD chip) with your hand or have any object come
in contact with glass surface. Should dust stick to the cover slip, blow it off gently with
an air blower. (For dust stuck due to static electricity, ionized air is recommended.)
Warning
Do not subject the camera to too much mechanical shock.
Warning
If the FireWire cable is damaged or severed, you risk an electric shock if you come
in direct contact with it. Shut off the hardware and change the FireWire cable immediately!
3
Warning
Important information
Warning
To prevent the microscope from toppling down, avoid using microscope attachments
that may make the total height of the microscope above 1 m when they are attached.
Operating
Environment
• Do not expose to strong light (sunlight) for long periods.
• High temperatures (> 40°C) and humidity (> 80%) can influence the camera's
capabilities. Avoid storage or usage under such conditions.
Start up
• The FireWire cable plug is not symmetrical. You must make sure that the cable
•
plug is facing the same way as the camera socket when you plug the cable into
the camera jack.
Do not disconnect the FireWire cable while the TWAIN Interface or the
DP2-BSW software is running.
Maintenance and Storage
1) To clean the lenses and other glass components, simply blow dirt away using a
commercially available blower and wipe gently using a piece of cleaning paper
(or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it with
gauze slightly moistened with commercially available absolute alcohol.
Warning
Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electric sparks - for
example, electrical equipment that is being switched on or off. Also remember to
always use these chemicals only in a well-ventilated room.
2) Parts other than the glass components should be cleaned by wiping with a clean
cloth. Do not use organic solvents to remove major stains. Use a soft cloth
slightly moistened with a neutral detergent solution.
3) Do not disassemble any part of the camera as this could result in malfunction or
reduced performance.
Conformity of the System
4
Restrictions
1) The recommended camera adapter is the U-TV0.5XC-3, U-TV0.63XC, MVXTV0.63XC or the combination U-TV1X-2 + U-CMAD3.
The U-TV0.5XC should not be used because it deteriorates the image flatness.
A camera adapter with a magnification below 0.5X cannot be used because part
of image will be cut off.
2) When the D25 is connected to the rear port of the U-DPT or U-MPH, the peripheral part of the recorded image may be deteriorated due to the optical performance of the U-DPT or U-MPH.
3) When the U-TV0.5XC-2 or U-TV0.5XC-3 is used, using two or more intermediate attachments* may obscure or cut off the peripheral part of the field of
view or may make flare noticeable.
Important information
* Example of two intermediate attachments: BX microscope + Vertical illuminator + Intermediate attachment with a length equivalent to the U-CA
4) Under fluorescent ring illumination or other AC-driven illumination such as a
phase control light intensity adjusting illumination system, the following phenomena may be observed when the light intensity is increased and exposure
time is decreased:
• Flickering of the displayed image.
• Instability in exposure.
However, provided that the brightness can be adjusted using the light intensity
control knob is possible or ND filters, the above phenomena may be attenuated
by adjusting the brightness so that the exposure time exceeds 20 ms.
For details on the microscope models using AC-driven illumination, consult
Olympus.
5) Non-Olympus microscopes and commercially available C-mount lenses can be
used provided that they match a CCD with a size of no less than 2/3 inch and
the lens projection length from the C-mount body attaching section is no more
than 6 mm. However, problems due to optical adaptability, such as shading,
may be observed.
6) When the specimen has a low contrast (near transparent) or high reflectance
(mirror status) and the aperture iris diaphragm is stopped down near the smallest aperture, spot flare may be noticeable.
7) When the edge of a non-transmitting object is observed under the STM6 transmitted illumination, flare may be noticeable due to the difference in brightness
between the transmitted sections (over-exposure) and non-transmitting section
(underexposure).
To reduce the flare, set a lower exposure using the exposure correction function
or setting the exposure manually.
8) When a low-power objective (below 4X) is used, the peripheral part of the field
of view may be obscured. In this case, use an ultralow-magnification condenser
(U-ULC-2).
9) This system cannot photograph dark specimens in reflected light fluorescence
or darkfield observation (a specimen that required exposure of 1/2 sec. or more
when the ISO is equivalent to 100).
11) Do not shut the aperture iris and field iris diaphragms of the microscope more
than required.
Operating Environment
Temperature: 5° to 40° C. Humidity: 5% to 80% (without condensation).
5
10) When using a stereo microscope at low zoom magnification, insufficient peripheral illumination may lead to the image brightness shading and/or vignetting.
System Chart
System Chart
Please note: Microscopes that are not listet in the system chart may also be applicable. For details, please
consult Olympus.
DP25
Camera Head
Monitor
IEEE 1394a
FireWire Cable
IEEE 1394a
FireWire PCI Board
DP2-BSW
Basic Software
DP25
TWAIN Driver
GX-TV0.5XC
C-Mount
Camera Adapter
U-CMAD3
C-Mount
Adapter
U-TV1X-2
Camera
Adapter
PC
GX-TV0.7XC
C-Mount
Camera Adapter
U-TV0.5XC-3
C-Mount
Camera Adapter
U-TV0.63XC
C-Mount
Camera Adapter
MVX-TV0.63XC
C-Mount
Camera Adapter
6
MVX-TV1 XC
C-Mount
Camera Adapter
STM6
Measuring
Microscope
IX81/IX71/IX51
Inverted
Microsope
MVX10
BX61/BX51/BX41
CX41/CX31
MX80/MX50/MX40
MX61L/MX61/MX51
Microscope
SZX16/SZX10
SZX12/SZX9
SZX7/SZ61
Stereo
Microscope
GX71/GX51
Inverted
Metallurgical
Microscope
Nomenclature
Nomenclature
Camera Head
Connector
C-Mount thread
FireWire PCI Board
(PCI standard half size)
Connectors
7
DP25 TWAIN driver (CD-ROM)
IEEE 1394a
FireWire PCI Cable
Installation and operation of the DP25 for TWAIN
Installation and operation of the DP25
for TWAIN
With the TWAIN Interface you control the acquisition of images. You can open the
TWAIN Interface from every application program that supports TWAIN. In this way,
you can easily acquire, look at, and edit images, with a software that you are familiar
with, for example, with Microsoft Word®.
Warning
Please install the TWAIN driver software (TWAIN Interface) before connecting the
camera to the FireWire interface.
System Requirements
To run your DP25 you have to use the Microsoft Windows XP operating system.To
make proper use of your DP25 via TWAIN, the following system requirements for
your PC have to be observed:
8
Hard- / Software
Required
Recommended
CPU
Pentium 4, 2.4 GHz or higher
Pentium M, 1.4 GHz or higher
Pentium D930, 3.0 GHz, Dual Core
Pentium M, 2.6 GHz
Hard Disk Storage
5 GB or more
20 GB
Memory
512 MB or more
1024 MB
Operating system
Microsoft Windows XP Pro SP 2
Ports
IEEE 1394a (Firewire™)
6 pin type
Required power supply capacity : 12 V, 0.2 A
Application program
Application program that supports TWAIN
Installation and operation of the DP25 for TWAIN
Installation of the camera
PLEASE NOTE Please read this chapter carefully before you begin the installation.
If you wish to run your camera with TWAIN, you will need to complete the following
steps.
Check if a FireWire interface is available on your PC.
If necessary, install a FireWire PCI board.
(The FireWire PCI board is included in your camera package.)
Install the TWAIN Interface.
Mount the camera on your microscope and connect it to your PC.
Select the TWAIN driver.
Start the application program that supports TWAIN.
9
Installation and operation of the DP25 for TWAIN
Checking if a FireWire interface is available on
your PC
If your PC already has a FireWire interface onboard or a separate FireWire PCI board
installed, you do not need to install the board provided with the camera.
If you are not sure whether a FireWire interface is available, open the Microsoft Windows Device Manager via Start > Control Panel > System > System Properties >
Hardware > Device Manager... . Check that the IEEE 1394 Bus host controllers entry
is there.
10
Installing the FireWire PCI board IEEE 1394a
Warning
When you install the FireWire PCI board, sharp edges may cut your fingers, so take
extra care.
1) Turn off the PC and disconnect the power supply.
2) Remove the cover from the PC.
3) One PCI slot is required for the FireWire board. Insert the board into the slot.
Installation and operation of the DP25 for TWAIN
FireWire PCI board
installed
4) Replace the cover on the PC.
5) Restart your PC.
Installing the TWAIN Interface
Warning
Please install the TWAIN driver software (TWAIN Interface) before connecting the
camera to the FireWire interface.
1) Put the CD-ROM that has been supplied into your CD-ROM drive.
" The installation program will start automatically - unless you have deactivated the autorun function. If so, please start the setup.exe file manually.
11
Installation and operation of the DP25 for TWAIN
The TWAIN installation
will be prepared.
12
A dialog box with the
license text will appear
automatically.
2) Read the license agreement carefully.
If you agree, select the Yes ... option and click the Next > button.
Installation and operation of the DP25 for TWAIN
The Customer Information dialog box will open.
3) In the Customer Information dialog box, enter your name and your company
name.The name, that has been used for the registration of the operating
system, will be suggested. In this dialog box, you also specify the users who will
be allowed to use the TWAIN interface on your PC.
4) Click the Next > button.
The Choose Destination
Location dialog box will
open.
13
Installation and operation of the DP25 for TWAIN
5) If you want the change the directory on which the TWAIN interface is to be
installed, click the Browse... button. Select the desired directory. You should
only change the suggested directory if there is an important reason for it.
6) Click the Next > button.
The Start Copying Files
dialog box will open.
7) To start the actual installation click the Next > button.
14
The installation of the
TWAIN Interface has
been completed. Click
the Finish button to exit
the Setup Wizard dialog
box.
8) Click the Finish button to finalize the installation.
" The TWAIN Interface has now been installed.
Installation and operation of the DP25 for TWAIN
Mounting the camera and selecting the camera
driver
Warning
Please install the TWAIN driver software (TWAIN Interface) before connecting the
camera to the FireWire interface.
Mounting the camera
1) Remove the protective cap from the DP25.
2) Screw the camera adapter securely to the DP25 camera. This should be done
with the CCD chip side of the camera facing down, to minimize the chance of
dust landing on its surface.
3) Install the camera and the camera adapter onto the microscope's camera port.
You may also need to align the orientation of the camera with that of the microscope stage, and also match the focus of the camera image with that of the eyepiece image. This alignment can be made later when you are able to obtain an
image on the screen.
Selecting the camera driver
4) Plug either end of the FireWire cable into the camera connector.
PLEASE NOTE The FireWire cable plug is not symmetrical. You must make sure that the cable plug
is facing the same way as the camera socket when you plug the cable into the
camera jack. The camera chip may be ruined otherwise.
Make sure the cable
plug is facing the right
way before you plug it
into the camera socket.
6) You then answer the question Can Windows connect to Windows Update to
search for software?, with No, not this time. Then click the Next> button.
15
5) Plug the other end into one of the free connector sockets on your PC's FireWire
PCI board.
" There are two or more sockets depending on the FireWire board. You can
use any of them.
" The Found New Hardware Wizard dialog will automatically appear.
Installation and operation of the DP25 for TWAIN
You then answer the
question Can Windows
connect to Windows
Update to search for
software?, with No, not
this time.
" A new dialog box entitled Found New Hardware Wizard will open.
7) Select the Install from a list or specific location (Advanced) option, and click the
Next > button.
16
In this dialog box, select
the Install from a list or
specific location
(Advanced) option.
" A new dialog box entitled Found New Hardware Wizard will open.
Installation and operation of the DP25 for TWAIN
8) Select the Don’t search. I will choose the driver to install option, and click the
Next > button.
In this dialog box, select
the Don’t search. I will
choose the driver to
install option.
" A new dialog box entitled Found New Hardware Wizard will open. It shows
the correct OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard)
driver.
The correct FireWire
camera driver is displayed in the Model list
of this dialog box.
17
Installation and operation of the DP25 for TWAIN
9) Select the OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard)
entry in the list.
10) Click the Next > button.
" The next Found New Hardware Wizard dialog box will be opened in the
background, displaying the message to wait while the Wizard installs the
software.
The Hardware
Installation dialog box
shown here may
pop up.
18
11) As the information given in this dialog box is not important for the installation,
simply click the Continue Anyway button.
" The Hardware Installation dialog box closes.
" The Found New Hardware Wizard dialog box which had been in the background, comes to the foreground.
Installation and operation of the DP25 for TWAIN
12) When the camera driver for the DP25 has been selected, the following dialog
box will open.
19
13) Click the Finish button to end the Found New Hardware Wizard.
" The DP25 is now ready for use.
Installation and operation of the DP25 for TWAIN
Starting the application program
Start the application program of your choice that supports TWAIN.
" The DP25 is now registered as a TWAIN device and can be selected.
Using the TWAIN Interface
Select the DP25 as a TWAIN source, and start the image acquisition. The exact
name of the commands, used to select the TWAIN source and start the image acquisition, depends on the application program you are using. For more detailed information please refer to the user’s manual of the application program you are using.
" The Olympus Twain window will open. This is the TWAIN Interface.
" The TWAIN Interface's image window displays the live-image.
Camera Control
20
Image Window
The TWAIN Interface's image window displays the live-image.
Installation and operation of the DP25 for TWAIN
Acquiring images
Click the Snap button.
" The image will be transferred to the software application from which you
started TWAIN. You can edit or save it there.
On the right-hand side
of the Olympus Twain
window, you will find all
of the controls you need
to optimally adjust
images.
Perform a white balance,
Use Shift Metering Regions
Close TWAIN Interface /
Acquire Image
Select exposure time mode and
Set exposure time
Select resolution (and Binning)
Use focus indicator
Trim the image (subarray)
Open the Camera Settings dialog box
An automatic exposure time mode, which will already produce excellent results for a
great variety of acquisitions, is preset. Depending on the properties of your sample,
however, it can become necessary to switch to the manual exposure time mode (e.g.,
samples with a strongly reflecting surface).
If you want to change the exposure time mode, click the option you want in the Exposure group (either Manual or Automatic).
21
Adjusting the exposure time
Installation and operation of the DP25 for TWAIN
Manual exposure In the manual exposure time mode you can enter the exposure time yourself. This
time mode mode can be recommended, for example, when you want to make a variety of different acquisitions of a sample to be able to compare their brightness.
This is how you set the exposure time manually
You have the following options:
• Use the slide controls.
• Click the plus and minus buttons.
• Click the arrow buttons at the right-hand end of the edit field.
• Enter an exposure time in the edit field, then press the [Enter] key.
In the manual exposure
time mode you can
select the exposure time
yourself.
" The exposure time will be adjusted accordingly.
If you wish, the TWAIN Interface can supply you with a benchmark, with the help of
which you will be able to set a suitable exposure time. To obtain this, click the One
time Auto Exposure button.
" The value set will be that which the automatic exposure time mode currently
supplies. You can then adjust this value as you wish.
Automatic In the automatic exposure time mode, the TWAIN Interface automatically defines the
exposure time exposure time for each single acquisition, so that the brightest part of the image
mode always remains the same. This way you can always see the optimal image.
22
In automatic exposure
time mode the exposure
time is constantly
checked and updated
automatically.
This is how you set the exposure time automatically
If it is not already selected, click the Automatic option. You will find this option in the
Exposure group.
" The TWAIN Interface will then calculate the exposure time automatically.
PLEASE NOTE In the camera settings, maximum exposure times that limit the upwards search for
the correct exposure times, are defined. You can see these values in the Camera
Settings dialog box, and can also change them there. Click the Settings button in the
camera control. Activate the DP25 > Exposure option. Then you will see the Maximum exposure time group.
Installation and operation of the DP25 for TWAIN
This is how you configure the automatic exposure time
Click the Lock Current Exposure Time button to freeze the current exposure time.
" Even when you move to a completely different place on your sample which
exhibits a different brightness, the exposure time will not be adjusted
accordingly.
" The Lock Current Exposure Time button will remain active (also for later
images), until you switch it off again.
Region If you want to, you can determine that the exposure time will only be measured on a
part of the image, you do this in the Region field. Select an entry from the picklist.
" If you have selected a spot, you have to define the Region of Interest (ROI)
on which the exposure time is to be measured, in the image.
" Should you want to position the spot in the center of the image, you should
use the Center Spot on Image button for this.
" Select the Full Image entry, if the exposure time is to be measured on the
whole image.
23
Adjustment If you want to, you can select a value from the Adjustment picklist. With this value
you can correct the automatic exposure time that has been calculated by the TWAIN
Interface.
" To correct the automatic exposure time, switch to the live-mode and select
a value from the Adjustment list. The largest possible correction value is 4,
the smallest -4.
" Should your images be overexposed or appear to be too bright, when you
use the automatic exposure time, select a negative correction value.
Should your images be underexposed when you use the automatic exposure time, select a positive correction value.
Select the value 0, if there is no need to correct the automatic exposure time.
" Test the effect of the exposure time correction in the live-image.
Installation and operation of the DP25 for TWAIN
Carrying out a white balance
Before an acquisition you must carry out a white balance if the colors are not correctly
reproduced. When using white balance, the individual color channels are scaled in
such a way that the white or neutral gray area of the image displayed on the monitor
is displayed correctly as white or gray.
Carrying out a white balance
1) Click the White Balance on ROI button. You will find this button above the Close
and Snap buttons.
2) In the image window, draw a frame with the left mouse button depressed.
The area within this frame is set to white. Change the position and the size of
the frame in such a way that the image area covered by the frame is well suited
for performing the white balance.
3) When you are satisfied with the frame's position, click the right mouse button
and select Confirm Input. Alternatively you can doubleclick in the frame.
" The settings for the individual color channels will be optimized in the set
area. You can see the results immediately in the image.
Selecting the resolution
Your DP25 can acquire images with a variety of different resolutions. The following
resolutions are available: 2560 x 1920, 1280 x 960 (binning), 854 x 640 (binning) and
640 x 480 (binning).
24
The following
resolutions are available: 2560x1920,
1280x960, 854x640 and
640x480.
By default, the "2560x1920" option is always preset. When the highest resolution has
been set, all of the data the camera has acquired will be read out, and therefore the
image will contain the most information.
The other resolutions require lower exposure times.
Changing the To alter the resolution, select the value you want from the Snap Resolution picklist.
resolution
Installation and operation of the DP25 for TWAIN
Trimming the image (subarray)
If you are only interested in a part of the image on display, you can determine that
only the data pertaining to this part of the image will be read out of the camera. You
can define which part that is in the image.
With a subarray you limit the amount of data that is exported from the camera to the
TWAIN Interface. You trim the image already before the acquisition. This will
increase the frame rate, and reduce the file size of the acquired images.
This is how you set up a subarray
1) Define which part of the image you are interested in. To do so, click the Set ROI
for Subarray button.
" The last previously defined ROI (Region of Interest) will be displayed.
2) Move the ROI to the place in the image that interests you. To do this, click in the
ROI, then with the left mouse button depressed, drag it to the place you want it.
3) Then adjust its size. To do this, drag the handles of the ROI. Confirm the ROI
either by doubleclicking in it, or by selecting the Confirm Input command in the
context menu. Rightclick to open the context menu.
" By doing so, you switch the subarray on. The image will then only show the
image segment you have defined.
This is how you switch off a subarray
Click the Toggle Subarray button to switch the subarray method on or off.
Focusing the sample
This is how you bring the sample into focus by using the Focus Indicator
1) Go to the place in the sample that interests you.
2) Click the Toggle Focus Indicator button to switch the Focus Indicator on.
" The sharpness monitor in the Focus Indicator will appear.
" A yellow rectangle will appear in the image. Only the pixels that are within
this rectangle will be taken into account for the measurement of the sharpness of your image.
25
Focus Indicator Use the Focus Indicator to bring the sample into focus. Unlike the human eye, the
Focus Indicator calculates the sharpness of the image on a mathematical basis.
The Focus Indicator enables you to control the focus settings. To begin with, you
define a rectangular area of the image that is to be analyzed for this calculation. The
maximum contrast serves as a monitor for the sharpness in this area.
The Focus Indicator has a display that indicates the relative degree of sharpness by
means of two superimposed bars. One of these bars is red, the other blue. The red
bar shows the greatest sharpness that has currently been achieved. The blue bar
shows the sharpness that is being achieved with the current focusing.
Installation and operation of the DP25 for TWAIN
3) Change the position and size of the rectangle, so that an area of the sample that
especially interests you will be measured. Please, make sure that the area
within the yellow rectangle shows a high contrast portion of the sample.
4) Turn the precision focusing knob on your microscope to change the focusing.
While doing this, watch the display in the Focus Indicator.
" The red bar shows the greatest sharpness that has currently been achieved.
" The blue bar shows the sharpness that is being achieved with the current
focusing.
5) Change the focusing on your microscope until the display is as completely blue
as possible.
6) Click the Reset Focus Indicator button, to reset the sharpness monitor's bars at
the relative value of 75% for a better visualization.
PLEASE NOTE A binning that has been set does not influence the functionality of the Focus
Indicator.
Zooming the image
The image located in the TWAIN interface’s image window can be zoomed in or out.
Zooming in the If your mouse is equipped with a mouse wheel, turn the mouse wheel backwards to
image zoom in the image. Or, press the [Crtl] key and then click the left mouse button.
Zooming out the If your mouse is equipped with a mouse wheel, turn the mouse wheel forwards to
image zoom out the image. Or, press the [Crtl + Alt] keys simultaneously and then click the
left mouse button.
Shift Metering Regions
26
For two of the DP25 functions you will need to define a rectangular image area. One
of these functions is the Spot mode, used for the calculation of the automatic exposure time, the other is the Focus Indicator.
The areas you have defined can, at any time, be moved or changed in size. Release
the Shift Metering Regions button when you want to fix the position of these areas on
an image.
Settings
If you want to change the camera settings, click the Settings... button. The dialog
box's tree view offers you access to the General, Color, Adjustment, Exposure and
Information dialog boxes. Select an entry in the tree view to load the respective dialog
box.
Click the Default button to return the settings in the active dialog box to their default
values. The current settings will then be lost. Confirm your settings with OK.
Installation and operation of the DP25 for TWAIN
General
Use this dialog box in the Camera Settings to make a variety of general camera settings. You can, for example, mirror the camera image, or select a pseudo color table.
The General dialog box
Mirror Select the Horizontal check box to mirror the image horizontally during acquisition.
Select the Vertical check box to mirror the image vertically during acquisition.
The two forms of mirroring can be combined with each other.
Image Type Select the default option "24 bit RGB Color", to acquire images of the "24-bit truecolor" type.
Select the "8 bit Gray Scale" option to acquire images of the "8-bit gray-value" type.
27
Pseudo Color You can use pseudo color tables to have images displayed in color on your monitor.
By default, when you acquire images, no pseudo color tables will be used. In this
case, the Pseudo Color > Off option is selected.
Select the Use high/low pseudo color table option to have all of the pixels with a low
intensity value displayed in blue, and all of those with a high intensity value displayed
in red. When you acquire this image, it will show all of the pixels in their actual color.
With this pseudo color table you can immediately see if the image is well illuminated.
When there are both blue and red pixels in the image, the camera's complete
dynamic range will be in use. If too many blue pixels can be seen, the image is underexposed. If too many red pixels can be seen, the image is overexposed.
Installation and operation of the DP25 for TWAIN
Color
Use this dialog box in the Camera Settings to change the camera's color settings
manually.
In this way you can change the Parameters Gain, Offset and Saturation individually
for each color channel. To do this, move their slide controls. R, G and B stand for the
Red, Green and Blue color channels.
The Color dialog box
Gain With the Gain (White Balance) value, you can weight the individual color channels
(White Balance) separately. Increase, e.g. the R value, to increase the amount of red in the image.
28
Saturation Increase the Saturation value, if the color is too pale, and you want a better color
saturation.
Installation and operation of the DP25 for TWAIN
Adjustment
Use this dialog box in the Camera Settings to change the camera's contrast settings
manually.
The Adjustment dialog
box
You will change the settings for all color channels simultaneously. If you want to
accentuate or tone down one particular color channel, use the functions in the Color
dialog box.
Gamma Gamma increases, respectively lowers the image contrast, subject to the intensity I
of a pixel, according to the following equation.
I0 = I
1⁄γ
Sharpness Use the sharpen filter to increase or decrease the sharpness of the image by edge
enhancement. You change the strength of the sharpen filter by moving its slide control.
Values ranging from -30 to 30 are possible, whereas the default value is 0. The image
gains a smoother appearance when you use negative values and a sharper one
when you use positive values. Raising sharpness will accentuate edges, but also
increases image noise. Entering a value of 0 will result in the sharpen filter having no
effect at all.
29
with I = intensity value of the destination image, I0= the intensity value of the source
image.
The values for Gamma vary between 0.2 and 5. Values smaller than 1 make the
image altogether darker. At the same time, the contrast in bright areas of the image
will be increased. Values greater than 1 make the image altogether brighter. At the
same time, the contrast in dark areas of the image will be increased.
Installation and operation of the DP25 for TWAIN
Gain The intensity value will be multiplied by the Gain value. The image will then become
altogether brighter.
Contrast Move the Contrast slide to change the contrast values of the image. Values smaller
than 1 lower the image contrast. Values greater than 1 enhance the image contrast.
When changing the image contrast the brightest part of the image always remains
the same. After changing the image contrast it may be that dark or bright image areas
are not displayed optimally anymore. In this case use the Brightness slider to make
the whole image brighter or darker.
Brightness Use the Brightness slider, if the whole image is too bright or too dark. Values smaller
than 0 lower the image brightness. Values greater than 0 increase the image brightness.
Shading correction
In the Shading Correction group you can correct two different shadings. First of all,
the shading correction corrects the effect of an inhomogeneous illumination
(Illumination correction). Additionally, the shading correction corrects the different
sensitivity of individual pixels on the CCD chip (Noise correction).
Select the Dark option to activate the Noise Correction. Select the Flatfield option to
activate both the Illumination Correction and the Noise Correction. Select the Off
option to disable the a shading correction.
Click the Edit... button to check whether correction images have already been
acquired or not. If correction images exist, you will find them listed in this dialog box.
In the Image Type column the correction images used for the Noise Correction are
indicated by the Dark entry, the correction images used for the Illumination Correction by the Flatfield entry.
30
In this dialog box check,
whether there are
correction images of the
Dark or Flatfield type, or
not.
Definition Every optical system with a camera and microscope creates, even when the devices
"Shading" have been set up very carefully set up, image inhomogeneities in the illumination of
the sample. These are called shading.
Installation and operation of the DP25 for TWAIN
The left illustration
shows an image where
the online shading
correction has not been
activated. In comparison to the image on the
right, one can notice the
inhomogoneities in the
illumination.
For a shading correction, the shading will first be determined in order to be able to
make corrections to the acquired image. To determine which shading there is in the
image, two types of correction images will be acquired:
Dark correction The Dark correction image is an acquisition during which no light falls on the camera.
image Here, pixels with different brightness resulting from noise or different sensitivity of
individual pixels on the camera chip can be corrected by the correction image. The
Dark correction image is characteristic for each camera and need only be acquired
once. It is valid for all objectives.
Flatfield correction On the Flatfield correction image the illumination of the complete optical system withimage out a sample will be shown. An individual correction image must be made for every
objective and for every resolution of your camera.
PLEASE NOTE You will need to acquire new illumination correction images every time you make
changes in your optical system. For example, when you clean your microscope,
change the objectives or change the camera.
Acquiring images for the shading correction
1) Open the Camera Settings > Adjustment dialog box. Click the Calibrate...
button.
" The Shading Correction wizard will open.
31
Installation and operation of the DP25 for TWAIN
The wizard leads you
step by step through the
calibration of the
shading correction.
2) If you have already acquired a Dark correction image, the Skip recording dark
correction image check box will be automatically selected.
Clear the check box, if you want to acquire a new Dark correction image.
Click the Next > button.
32
The Dark correction
image is an acquisition
during which no light
falls on the camera. This
image is subtracted
from the acquired
image.
3) Then follow the instructions given you by the software wizard. Close the light
path selector knob on your microscope, so that no light can fall on the camera.
Then click Next.
" The Dark correction image will be acquired.
" The next dialog box of the wizard will open.
Installation and operation of the DP25 for TWAIN
On the Flatfield
correction images the
illumination of the complete optical system
without a sample will be
shown.
PLEASE NOTE The Flatfield correction image depends on the objective being used. Correspondingly, an individual correction image must be made for every objective. If you want to
use another objective for acquiring images, you will have to acquire a new Flatfield
correction image.
4) Move to a position where is no sample and no scratches, and defocus.
5) Click the Next button and acquire the correction images for the currently
selected objective.
" The TWAIN interface automatically acquires the correction images for all
camera resolutions.
33
6) Click the Finish button to finalize the calibration and return to the Camera Settings > Adjustment dialog box.
Installation and operation of the DP25 for TWAIN
Exposure
Use this dialog box in the Camera Settings to set the maximum possible exposure
times for the image acquisition. Set an upwards limit to the maximum possible exposure time, to make sure that the live-image is in any case quick enough to be able to
focus comfortably (also see "Adjusting the exposure time" on page 21).
The Exposure dialog
box
Information
34
Use this dialog box in the Camera Settings to view information about your
DP25 camera.
Installation of the DP25 for the DP2-BSW software
Installation of the DP25 for the
DP2-BSW software
System Requirements
To run your DP25 you have to use the Microsoft Windows XP operating system.To
make proper use of your DP25, the following system requirements for your PC and
the DP2-BSW software have to be observed:
Hard- / Software
CPU
Required
Pentium 4, 2.4 GHz or higher
Pentium M, 1.4 GHz or higher
Recommended
Pentium D930, 3.0 GHz,
Dual Core
Pentium M, 2.6 GHz
Hard Disk Storage
5 GB or more
20 GB
Memory
512 MB or more
1024 MB
Operating system
Microsoft Windows XP Pro SP 2
Ports
IEEE 1394a (Firewire™)
6 pin type
Required power supply capacity 12 V, 0.2 A
Image analysis
application
DP2-BSW
35
Installation of the DP25 for the DP2-BSW software
Installation of the camera
In order to avoid problems, you will have to follow the steps of the procedure in the
order given here.
Check if a FireWire interface is available on your PC.
If necessary, install a FireWire PCI board.
(The FireWire PCI board is included in your camera package.)
Install the DP2-BSW program on your PC.
(The camera driver will also be automatically installed.)
Mount the camera on your microscope and connect it to your PC.
Select the camera driver.
36
Start the DP2-BSW software.
Checking if a FireWire interface is available on
your PC
If your PC already has a FireWire interface onboard or a separate FireWire PCI board
installed, you do not need to install the board provided with the camera.
If you are not sure whether a FireWire interface is available, open the Microsoft Windows Device Manager via Start > Control Panel > System > System Properties >
Hardware > Device Manager... . Check that the IEEE 1394 Bus host controllers entry
is there.
Installation of the DP25 for the DP2-BSW software
Installing the FireWire PCI board IEEE 1394a
Warning
When you install the FireWire PCI board, sharp edges may cut your fingers, so take
extra care.
1) Turn off the PC and disconnect the power supply.
2) Remove the cover from the PC.
3) One PCI slot is required for the FireWire board. Insert the board into the slot.
37
Installation of the DP25 for the DP2-BSW software
FireWire PCI board
installed
4) Replace the cover on the PC.
5) Restart your PC.
Installing the DP2-BSW software
Warning
Please install the DP2-BSW software before connecting the camera to the FireWire
interface.
Should the DP25 have already been connected to the PC before the DP2-BSW software was installed, Microsoft Windows XP will have installed its own driver, which will
prevent the installation of the correct driver.
Please note that this does not apply to the DP20! Should you decide to use the DP20
instead of the DP25, the PC will not automatically install the camera driver. When
using the DP20 with the DP2-BSW software, you will need to manually install the
camera driver from the CD-ROM.
38
You can install the DP2-BSW software on your PC even if you have already installed
another software package from Olympus Soft Imaging Solutions on it.
1) Insert the software protection key (dongle) into a USB port of your PC.
Installation of the DP25 for the DP2-BSW software
Your DP2-BSW software is protected by a
dongle. Insert it into a
USB port of your PC.
2) Turn on your PC and, if necessary, start up the operating system.
" The Found New Hardware dialog box will open. The operating system will
then try to install a driver for the dongle.
When you have plugged
in the dongle, this dialog
box will appear.
39
3) Since the correct dongle driver can only be installed during the installation of the
image analysis program, you will then need to click the Cancel button.
" The Found New Hardware dialog box will close. During the installation of
your image analysis program, the correct dongle driver will be automatically
installed.
4) Close any and all application programs.
5) Put the installation CD-ROM into your CD-ROM drive.
Installation of the DP25 for the DP2-BSW software
" The installation program will open automatically - unless you have deactiva-
ted the auto run function. If so, start the setup.exe file via Microsoft Windows
Explorer.
6) To make the installation, simply follow the instructions of the installation wizard,
and each time you have made a necessary entry, click the Next > button to continue.
In the Image Source
Selection dialog box,
select Olympus from the
Manufacturer/Image
Source list and the
DP25 entry located in
the Device list.
40
The installation of the
DP2-BSW software is
complete. Click the
Finish button to exit the
InstallShield Wizard
Complete dialog box.
Installation of the DP25 for the DP2-BSW software
Mounting the camera
Warning
Please install the DP2-BSW software before connecting the camera to the FireWire
interface.
Mounting the camera
1) Remove the protective cap from the DP25.
2) Screw the camera adapter securely to the DP25 camera. This should be done
with the CCD chip side of the camera facing down, to minimize the chance of
dust landing on its surface.
3) Install the camera and the camera adapter onto the microscope's camera port.
You may need to align the orientation of the camera with that of the microscope
stage, and also match the focus of the camera image with that of the eyepiece
image. This alignment can be made later when you are able to obtain an image
on the screen.
PLEASE NOTE
4) Plug either end of the FireWire cable into the camera connector.
5) The FireWire cable plug is not symmetrical. You must make sure that the cable
plug is facing the same way as the camera socket when you plug the cable into
the camera jack. The camera chip may be ruined otherwise.
Make sure the cable
plug is facing the right
way before you plug it
into the camera socket.
41
6) Plug the other end into one of the free connector sockets on your PC's FireWire
PCI board. There are two or more sockets depending on the FireWire board.
You can use any of them.
Installation of the DP25 for the DP2-BSW software
Selecting the camera driver
Prerequisite You have installed the DP2-BSW software and then connected the DP25 camera to
the FireWire slot.
" The Found New Hardware Wizard dialog will automatically appear.
1) You then answer the question Can Windows connect to Windows Update to
search for software?, with No, not this time. Then click the Next> button.
You then answer the
question Can Windows
connect to Windows
Update to search for
software?, with No, not
this time.
" A new dialog box entitled Found New Hardware Wizard will open.
42
2) Select the Install from a list or specific location (Advanced) option, and click the
Next > button.
Installation of the DP25 for the DP2-BSW software
In this dialog box, select
the Install from a list or
specific location
(Advanced) option.
" A new dialog box entitled Found New Hardware Wizard will open.
3) Select the Don’t search. I will choose the driver to install option, and click the
Next > button.
In this dialog box, select
the Don’t search. I will
choose the driver to
install option.
43
Installation of the DP25 for the DP2-BSW software
" A new dialog box entitled Found New Hardware Wizard will open. It shows
the correct OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard)
driver.
The correct FireWire
camera driver is displayed in the Model list
of this dialog box.
4) Select the OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard)
entry in the list.
If this entry is missing, click the Cancel button. Repeat the installation of the
DP2-BSW software including the selection of the DP25 camera.
44
5) Click the Next > button.
" The next Found New Hardware Wizard dialog box will be opened in the
background, displaying the message to wait while the Wizard installs the
software.
Installation of the DP25 for the DP2-BSW software
The Hardware
Installation dialog box
shown here may pop
up.
6) As the information given in this dialog box is not important for the installation,
simply click the Continue Anyway button.
" The Hardware Installation dialog box closes.
" The Found New Hardware Wizard dialog box which had been in the background, comes to the foreground.
45
7) After completing the installation of the driver for the DP25 camera, the following
dialog box is opened.
Installation of the DP25 for the DP2-BSW software
8) Click the Finish button to end the Found New Hardware Wizard.
" The DP25 is now ready for use.
46
Starting the DP2-BSW software
Installation of the DP25 for the DP2-BSW software
Acquiring your first images
Prerequisites You have successfully installed your system (DP2-BSW software, camera, and
camera driver). You would like to acquire your first images.
1) Start the DP2-BSW software.
" You will automatically be in the Acquisition layout. This means that all of the
"
software controls are optimally positioned on your monitor for acquiring
images.
On the right-hand edge of your screen you will find the Camera Control tool
window. There, the Live and Snap buttons are active. You can now acquire
images and make changes in the camera settings.
Live
Click the Live button in the Camera Control tool window or use the Acquire > Live
command.
The acquired image is shown live in the document window.
Use the live-mode for focussing or when arranging the camera or the sample.
Snap
Click the Snap button in the Camera Control tool window or use the Acquire > Snap
command.
Use the Snap mode, to stop the live-image or to acquire a snapshot.
You can edit or save the image you have acquired. The way to make image acquisitions is described in detail in your DP2-BSW software manual.
47
Installation of the DP25 for the DP2-BSW software
Special camera functions
In the following you will find a description of two controls that can be found in your
DP2-BSW software's Camera Control tool window when you work with the DP25.
You will only find these two controls when you work with the DP25. All of the other
functions you will find described in detail in your DP2-BSW software's manual.
The DP2-BSW
program's Camera
Control tool window.
Here, only the two
functions that are
specific to the DP25,
"Field Update" and
"Trimming the image"
will be described.
48
DP25 > Field Update
Trim the image (subarray)
Installation of the DP25 for the DP2-BSW software
Trimming the image (subarray)
If you are only interested in a part of the image on display, you can determine that
only the data pertaining to this part of the image will be read out of the camera (Partial
Readout). You can define which part that is in the live-image.
With a subarray you limit the amount of data that is exported from the camera to your
DP2-BSW system. You trim the image already before the acquisition. The frame rate
of the live-image will then increase and the size of the file in which the acquired
images are saved will be reduced.
Setting a subarray
1) First you define which part of the image you are interested in. To do so, click the
Set ROI for Subarray button.
" The mouse pointer will appear within the image. The last previously defined
ROI (Region of Interest) will be displayed.
2) Move the ROI to the place in the image that interests you. To do this, click in the
ROI, then with the left mouse button depressed, drag it to the place you want it.
3) Then adjust its size. To do this, drag the handles of the ROI. Confirm the ROI
either by doubleclicking in it, or by selecting the Confirm Input command in the
context menu. Rightclick to open the context menu.
" By doing so, the subarray is switched on. The live-image will then only show
the image segment you have defined.
4) Then determine whether the subarray is only to be employed for displaying the
live-image, or also for the acquisition. If it is also to be employed for the acquired
image, clear the Live only check box.
" By default, the subarray will only be employed for the live-image, that is why
the Live only check box is preselected.
" The display will be updated.
Field Update
49
The Field Update check box switches between a simple and an enhanced frame rate.
The performance of the CPU in your PC determines the extend to which the frame
rate can be enhanced.
Select the Field Update check box to increase the frame rate. By default, the
enhanced frame rate is set. This means that the live-image will be quickly updated,
even if the image information changes quickly. However, this setting will require a
considerable amount of your CPU's performance.
Clear the Field Update check box, if an enhanced frame rate is not especially important for you. This will avoid blocking more of your CPU's performance than necessary.
Specifications
Specifications
DP25 Specifications
Item
Specifications
Camera
Single-CCD color camera
Image pickup device
2/3-inch color CCD
Total pixels: 5.24 million pixels
Effective pixels: 4.92 million pixels
Pixel pitch: 3.4 µm (H) x 3.4 µm (V)
Scanning method: Interline scanning
Cooling
Natural air cooling
Recorded image sizes
2560 x 1920 (1 x 1)
1280 x 960 (2 x 2)
854 x 640 (3 x 3)
640 x 480 (4 x 4)
Lens mount
C-mount
A/D
12 bits
Exposure control*
Exposure modes
Auto
Manual
AE lock
Available
One time AE
Available
Exposure correction**
Correction range: ± 4.0 EV. Steps: 1/3 (<2); 1/2 (>2)
Metering area
Full image
Crosshair
30%
1%
0.1%
Exposure time
200 µs to 16 s
Binning
2 x 2, 3 x 3, 4 x 4
50
Live frame rate**,***
Metering area is freely movable.
Field Update
2560 (H) x 1920 (V) (1 x 1): approximately 8 fps
854 (H) x 640 (V) (3 x 3): approximately 24 fps
640 (H) x 480 (V) (4 x 4): approximately 32 fps
Frame Update
2560 (H) x 1920 (V) (1 x 1): approximately 4 fps
1280 (H) x 960 (V) (2 x 2): approximately 8 fps
854 (H) x 640 (V) (3 x 3): approximately 12 fps
640 (H) x 480 (V) (4 x 4): approximately 16 fps
Color modes
Color (24 bits)
Gray scale ( 8 bits)
White balance mode*
White Balance on ROI
Gain (White Balance)*
Manual; Range: 1 to 10; Increment: 0.01
Offset (Black Balance)**
Manual; Range: 0 to 100; Increment: 1
Saturation*
Range: 0 to 100; Increment: 1
Specifications
Item
Specifications
Gamma*
Range: 0.2 to 5; Increment: 0.001
Sharpness filter*
Range: -30 to 30; Increment: 1
Gain*
Range: 1 to 5; Increment: 0.01
Contrast*
Range: 0.5 to 2; Increment: 0.01
Brightness*
Range: -100 to 100; Increment: 1
Focus indicator*
Contrast bar
Interval recording**
Recording time: 1 s to 9999 h; Fixed Interval: 1 s to 1000 h
Image file formats**
TIFF
BMP
JPEG
JPEG 2000
PSD
PNG
PC Interface
IEEE 1394a (FIrewire)
6 pin type
Required power supply capacity for DP25: 12 V, 0.2 A
Compatible OS
Microsoft Windows® XP Professional SP 2 (not compatible with
x64 Edition)
Dimension and weight
Camera head
86 mm (Ø) x 48 mm (H); approximately 350 g
IEEE 1394a board
121 mm (W) x 132 mm (D) x 21.5 mm (H); approximately: 60 g
IEEE 1394a cable
4.5 m length
Power consumption
12 V, 0.2 A
Operating environment
Indoor use
Ambient temperatures: 5 °C to 40 °C (41 °F to 104 °F)
Relative humidity: 5% to 80% (without condensation)
Supply voltage fluctuations by IEEE 1394a: ± 10%
* These items can be set from the DP2-BSW V1.2 or TWAIN Interface V1.1
** These items can be set from the DP2-BSW V1.2
*** The values of these items are variable depending on the exposure time setting
and the PC operating
51
Help with Problems
Help with Problems
You can solve most of your image problems by changing the camera settings. To
open the Camera Settings dialog box, click the Settings button located in the Camera
Control tool window. By default, you will find the Camera Control tool window on the
right-hand edge of your screen.
Your image doesn't look as good as it should
What should I do, when the live-image suddenly appears to be "frozen", and I
receive an "Error making a snapshot" message?
Check to see if the connector cable between the camera and the PC has been correctly plugged-in. Should the connector be loose or not plugged in correctly, correct
this by plugging it firmly into the corresponding jack. Restart the DP2-BSW software.
Should the problem still exist, contact your sales dealer.
What should I do if the image "quivers"?
Quivering is caused by a frame rate that is too low. Select a lower resolution and/or
a shorter exposure time. And increase the intensity.
What should I do when the image appears to be too dark?
52
• Check on your microscope whether a diaphragm has been closed too much, or
the beam splitter has been adjusted in such a way that no light can reach the
camera.
Should your image still be too dark, then please check the following points one after
the other.
• The exposure time is possibly too short. Select a longer exposure time!
" In the manual exposure time mode: Click the Settings... button in the
Camera Control tool window. In the Camera Settings dialog box select the
Exposure entry from the tree view. In the Camera Settings > Exposure
dialog box, check whether the maximum allowed exposure time has been
reached. If necessary, increase this value. To do so, move the Manual slide
control to the right.
" In the automatic exposure time mode: You should check in the Camera Settings > Exposure dialog box, whether the maximum allowed exposure time
has been reached. If necessary, increase the "Maximum exposure time"
value. To do so, move the Automatic slide control to the right. Alternatively,
you can also enter a higher value directly in the text field.
• It is possible that the exposure time correction has been set too low. In the
Camera Settings > DP25 > Exposure dialog box, click the Exposure Correction... button, and make sure that the exposure time correction does not lie
below 0. Should this exposure time correction show a negative value, then
please reset it to 0.
Help with Problems
What should I do when the image appears to be too bright?
• First of all, make sure that your microscope is correctly set up (diaphragm
opening, beam splitter...).
Should your image still be too bright, then please check the following points one after
the other.
• The exposure time is possibly too long. Select a shorter exposure time!
" In the manual exposure time mode: In the camera control reduce the exposure time.
" In the automatic exposure time mode: The lowest possible exposure time of
the camera (200 µs) has possibly been reached.
In the Camera Settings > Adjustment dialog box set the Gamma, Sharpness, Gain, Contrast, and Brightness values at their resp. default values. To
do this, click the Default button.
• In the Camera Settings > DP25 > Exposure dialog box, click the Exposure
Correction... button, and make sure that the exposure time correction does not
lie above 0. Set the value to 0.
What should I do when the image is monochrome?
First of all, check which type of image has been set. Should the Settings > DP25 >
General > Image type > 24 bit RGB color option not have been set, then set it.
Should the image have been set as a "24 bit RGB color" type of image, but the image
is still monochrome, carry out a white balance with an appropriate sample.
Should your image show a red tinge, despite your having carried out a white balance,
this is because your lamp's color temperature is too low. Increase the lamp voltage.
As a next step, you should check the following settings on your PC and its monitor.
Via Control Panel > Display properties > Settings > Color Quality, set your PC's color
depth at a minimum value of 24 bits. Select, for example, the Highest (32 bit) option.
Set your monitor's color temperature either via the monitor's menu navigation or via
the operating system.
This is how you set the color temperature via the operating system
1) Rightclick on the desktop.
2) Select the Properties option in the context menu.
" The Display Properties dialog box will open.
4) Click the Advanced... button.
" A further Display Properties dialog box will open. The appearance and functions of this dialog box depend on the graphics card that has been installed
in your system, and on your monitor.
5) Look for a tab with either the name Color or Color Management.
6) Select a color temperature whose color impression as near as possibly matches
that of the image in the eyepiece.
53
3) Select the Settings tab.
Help with Problems
What should I do if the image is blurred and/or lacks contrast?
Focus the sample visually on your microscope. Set the camera adapter so as to
make the eye piece's focal plane identical to that of your camera.
Take as much care as possible to ensure that your camera and your microscope
optics are kept clean, and that the whole system is mechanically stable.
Make sure that the condenser's aperture stop is not open too wide.
"Live" and "Snap" are not active
What should I do when the "Live" and "Snap" buttons are inactive?
This chapter is only relevant, if you use the DP25 with the DP2-BSW software.
Check to see if the connector cable between the camera and the PC has been correctly plugged-in. Should the connector be loose or not plugged in correctly, correct
this by plugging it firmly into the corresponding jack. Restart the DP2-BSW software.
Should the problem still exist, continue with the following section.
In the Device Manager, check if there is an IEEE 1394 Bus host controllers entry
there, and also that the OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard) entry is there under the Imaging devices branch. Open the Microsoft Device
Manager via Start > Control Panel > System > System Properties > Hardware >
Device Manager.
Should these entries not be present, the driver will then have to be installed afterwards, independently from the installation CD-ROM (see "How can I reinstall the
camera driver?" on page 54).
Should the problem not have been solved even when the driver has subsequently
been successfully installed, check whether an Optical Path has been entered in the
Optical Path dialog box. You can open the Optical Path dialog box via the Acquire >
Devices > Optical Path... command.
54
Should no entry be displayed in the upper list in the Optical Path dialog box, set one
up by using the New Wizard button. Please refer to the online help of the DP2-BSW
software. There you will find a step by step instruction how to set up an Optical Path.
Should the problem still exist, contact your sales dealer.
When connecting the camera to the Firewire interface before
installing the DP25 TWAIN driver software or the DP2-BSW software
How can I reinstall the camera driver?
PLEASE NOTE Should the camera have already been connected to the PC before the TWAIN Interface or the DP2-BSW software was installed, Microsoft Windows XP will have
installed its own driver, which will prevent the installation of the correct driver. Installing the DP2-BSW software afterwards or reinstalling the TWAIN interface will be of
no help! The driver will have to be installed separately.
Help with Problems
1) Open the Microsoft Device Manager via Start > Control Panel > System >
System Properties > Hardware > Device Manager.
To open the MS DeviceManager, first click Start
then click Control Panel.
Then click the Device
Manager button on the
System Properties >
Hardware tab.
You can see that the
camera driver has been
located when the SoftHard Technology
MV4.0 entry is shown in
the Other devices
branch.
55
2) Select the SoftHard Technology MV4.0 entry in the Device Manager, and click
the Update Driver button in the Device Manager.
" The Found New Hardware Wizard dialog box appears.
3) The question Can Windows connect to Windows Update to search for software?
should be answered with No, not this time; after that click the Next> button.
Help with Problems
You then answer the
question Can Windows
connect to Windows
Update to search for
software?, with No, not
this time.
" A new dialog box entitled Found New Hardware Wizard will open.
4) Select the Install from a list or specific location (Advanced) option, and click the
Next > button.
56
In this dialog box, select
the Install from a list or
specific location
(Advanced) option.
Help with Problems
" A new dialog box entitled Found New Hardware Wizard will open.
5) Select the Don’t search. I will choose the driver to install option.
In this dialog box, select
the Don’t search. I will
choose the driver to
install option.
6) Click the Next > button.
The OLYMPUS CORPORATION FireWire
2.0.26 (SoftHard) entry
is offered in the Model
list located in this dialog
box. Select it.
57
7) Select the OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard)
entry in the Model list.
Help with Problems
8) Click the Next > button.
" The next Found New Hardware Wizard dialog box will be opened in the
background, displaying the message to wait while the Wizard installs the
software.
The Hardware
Installation dialog box
shown here may
pop up.
58
9) As the information given in this dialog box is not important for the installation,
simply click the Continue Anyway button.
" You will return to the Found New Hardware Wizard dialog box.
10) Click the Next > button.
Help with Problems
" After completing the installation of the driver for the DP25 camera, the
following dialog box is opened.
11) Click the Finish button to end the Hardware Update Wizard assistant.
12) Switch to the Device Manager.
" In the tree view, you will find the OLYMPUS CORPORATION FireWire
Camera 2.0.26 (SoftHard) entry under the Imaging Devices branch.
13) Restart the PC.
" The camera is now ready for use.
59
Help with Problems
60
After successfully
installing the driver for
the DP25, the
OLYMPUS CORPORATION FireWire Camera
2.0.26 (SoftHard) entry
is displayed in the
Device Manager dialog
box. The camera is now
ready for use.
Declarations of Conformity
Declarations of Conformity
WEEE declaration
Waste Electrical
and Electronic
Equipment
In accordance with European Directive 2002/96/EC on Waste Electrical
and Electronic Equipment, this symbol indicates that the product must not
be disposed of as unsorted municipal waste, but should be collected
separately. Refer to your local distributor for return.
FCC compliance
This equipment has been tested and found to comply with the limits for a Class A
digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to
provide reasonable protection against harmful interference when the equipment is
operated in a commercial environment. This equipment generates, uses, and can
radiate radio frequency energy and, if not installed and used in accordance with the
instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own
expense.
PLEASE NOTE Changes or modifications not expressly approved by the party responsible for
compliance could void the user’s authority to operate the equipment.
CE conformity
This device complies with the requirements of directive 89/336/EEC
concerning electromagnetic compatibility.
The CE marking indicates compliances with the above directive.
61
OLYMPUS CORPORATION
Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku- Tokyo, Japan
OLYMPUS LIFE AND MATERIAL SCIENCE EUROPA GMBH
Postfach 10 49 08, 20034, Hamburg, Germany
OLYMPUS AMERICA INC.
3500 Corporate Parkway, Center Valley, PA18034-0610, U.S.A.
OLYMPUS SINGAPORE PTE LTD.
491 B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373
OLYMPUS AUSTRALIA PTY. LTD.
31 Gilby Road, Mt. Waverley, VIC 3149, Melbourne, Australia
OLYMPUS LATIN AMERICA, INC.
5301 Blue Lagoon Drive, Suite 290, Miami, FL 33126, U.S.A.
OLYMPUS (BEIJING) SALES & SERVICE CO., LTD.
12-13F, NCI Tower, A12 Jianguomenwai Avenue, Chaoyang District, Beijing, 100022, China
Printed in Germany DP25_E_0706