FluorEssence™ User`s Guide for software version 3.5

FluorEssence 3.5 User’s Guide rev. C (26 Jan 2012)
FluorEssence™
User’s Guide for software
version 3.5
with Multigroup software
rev. C
http://www.Horiba.com/Scientific
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FluorEssence 3.5 User’s Guide rev. C (26 Jan 2012)
Copyright © 2012 by HORIBA Instruments Incorporated
All rights reserved. No part of this work may be reproduced,
stored, in a retrieval system, or transmitted in any form by any
means, including electronic or mechanical, photocopying and
recording, without prior written permission from HORIBA
Instruments Incorporated. Requests for permission should be
requested in writing. Origin® is a registered trademark of
OriginLab Corporation. RealPlayer® is a registered trademark of
Real Networks, Inc. InstallShield® is a registered trademark of
InstallShield Software Corporation. Windows® is a trademark of
Microsoft Corporation.
Information in this manual is subject to change without notice, and
does not represent a commitment on the part of the vendor.
January 2012
revision C
Part Number J810000
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FluorEssence 3.5 User’s Guide rev. C (26 Jan 2012)
Table of Contents
0: Introduction .............................................................4
About FluorEssence™ ........................................................................ 4
About Multigroup................................................................................. 4
Disclaimer ........................................................................................... 5
Symbols used in this manual .............................................................. 7
1: Installation .............................................................8
Requirements ..................................................................................... 8
Installation instructions ....................................................................... 9
Loading correction-factor files .......................................................... 25
2: Quick Guide to Running a Scan .................................... 29
3: Tips & Tricks ......................................................... 37
Calibration of your instrument ........................................................... 37
Using corrected signals .................................................................... 45
Projects and files .............................................................................. 46
Saving and recalling a file ................................................................. 52
4: Shutting Down ........................................................ 56
5: Multigroup Software ................................................ 57
About Multigroup............................................................................... 58
Requirements ................................................................................... 58
Installation......................................................................................... 59
Running Multigroup........................................................................... 62
Working with experiments and data ................................................. 67
6: Un-Installation ....................................................... 70
FluorEssence™ ................................................................................ 70
Multigroup ......................................................................................... 73
7: FluorEssence™ Troubleshooting & Technical Support......... 76
Troubleshooting ................................................................................ 76
On-line help ...................................................................................... 77
Frequently-asked questions about FluorEssence™......................... 78
Video tutorials ................................................................................... 79
If you have a technical problem, ....................................................... 80
Contact information........................................................................... 82
7: Index ................................................................... 83
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FluorEssence 3.5 User’s Guide rev. C (26 Jan 2012)
0: Introduction
About FluorEssence™
FluorEssence™ is the easiest data-acquisition software ever
created by HORIBA Scientific. All aspects of spectrofluorometer
control are available with only a few mouse-clicks or keystrokes,
with a minimum of overlapping screens and windows. Data can be
previewed while they are being recorded, and then immediately
used with Origin® presentation and graphical analysis.
FluorEssence™ runs using Windows® 2000 or higher.
About Multigroup
Multigroup is a special data-acquistion software in which multiple
steps can be automated. Repeating loops, delays, with multiplewavelength acquisition are possible. Multigroup runs using
Windows® 2000 or higher.
Note: Keep this and the other reference manuals near
the system.
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FluorEssence 3.5 User’s Guide rev. C (26 Jan 2012)
Disclaimer
By setting-up or starting to use any HORIBA Instruments
Incorporated product, you are accepting the following terms: You
are responsible for understanding the information contained in this
document. You should not rely on this information as absolute or
all-encompassing; there may be local issues (in your environment)
not addressed in this document that you may need to address, and
there may be issues or procedures discussed that may not apply to
your situation. If you do not follow the instructions or procedures
contained in this document, you are responsible for yourself and
your actions and all resulting consequences. If you rely on the
information contained in this document, you are responsible for:
Adhering to safety procedures
Following all precautions
Referring to additional safety documentation, such as Material
Safety Data Sheets (MSDS), when advised
As a condition of purchase, you agree to use safe operating
proceduresin the use of all products supplied by HORIBA
Instruments Incorporated, including those specified in the MSDS
provided with any chemicals and all warning and cautionary
notices, and to use all safety devices and guards when operating
equipment. You agree to indemnify and hold HORIBA
Instruments Incorporated harmless from any liability or obligation
arising from your use or misuse of any such products, including,
without limitation, to persons injured directly or indirectly in
connection with your use or operation of the products. The
foregoing indemnification shall in no event be deemed to have
expanded HORIBA Instruments Incorporated’s liability for the
products.
HORIBA Instruments Incorporated products are not intended for
any general cosmetic, drug, food, or household application, but
may be used for analytical measurements or research in these
fields, or for forensic applications. A condition of HORIBA
Instruments Incorporated’s acceptance of a purchase order is that
only qualified individuals, trained and familiar with procedures
suitable for the products ordered, will handle them. Training and
maintenance procedures may be purchased from HORIBA
Instruments Incorporated at an additional cost. HORIBA
Instruments Incorporated cannot be held responsible for actions
your employer or contractor may take without proper training.
Due to HORIBA Instruments Incorporated’s efforts to
continuously improve our products, all specifications, dimensions,
internal workings, and operating procedures are subject to change
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FluorEssence 3.5 User’s Guide rev. C (26 Jan 2012)
without notice. All specifications and measurements are
approximate, based on a standard configuration; results may vary
with the application and environment. Any software manufactured
by HORIBA Instruments Incorporated is also under constant
development and subject to change without notice.
Any warranties and remedies with respect to our products are
limited to those provided in writing as to a particular product. In no
event shall HORIBA Instruments Incorporated be held liable for
any special, incidental, indirect or consequential damages of any
kind, or any damages whatsoever resulting from loss of use, loss of
data, or loss of profits, arising out of or in connection with our
products or the use or possession thereof. HORIBA Instruments
Incorporated is also in no event liable for damages on any theory
of liability arising out of, or in connection with, the use or
performance of our hardware or software, regardless of whether
you have been advised of the possibility of damage.
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Symbols used in this manual
Certain symbols are used throughout the text for special conditions
when operating the instruments:
Note:
General information is given concerning
operation of the equipment.
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1: FluorEssence™
Installation
Requirements
To successfully install FluorEssence™, your host computer needs
the following:
Software
Windows® 2000, Windows® XP Pro, Windows® 7 (in
compatibility mode), or Windows® Vista
Hardware
Supports Windows® 2000, Windows® XP Pro, Windows® 7
(in compatibility mode), or Windows® Vista
1GB RAM
1 GB hard-disk space
One DVD-ROM drive
One available USB port
Video resolution of at least 1024 × 768
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Installation instructions
1 Remove any HORIBA USB software key (if
inserted) from the host computer before
starting the installation.
2 Insert the FluorEssence™ CD-ROM in the
host computer’s CD-ROM drive.
3 If Autorun is not operating, continue here:
a
On the desktop, open the My Computer
icon.
b
The My Computer window opens:
c
Double-click on the CD-ROM drive to open the
FluorEssence™ CD-ROM:
d
Click the Setup.exe icon.
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e
Continue with step 4 below.
4 If Autorun is operating, continue here, to
install FluorEssence™ software:
The InstallShield® Wizard starts.
a
Click the Next > button.
The License Agreement appears.
b
Click I accept the terms of the license agreement
radio button, then the Next > button.
The Customer Information area appears.
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Enter your User Name and Company Name. The Next >
button activates.
c
Click the Next > button.
The Choose Destination Location area appears.
d
Choose the location where FluorEssence™ is to be
installed.
Most people prefer the default location. Click the Change button to find a different location.
e
Click the Next > button.
The Ready to Install the Program area appears:
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f
g
Click the Install button.
The computer starts copying the files from the CD-ROM
to the hard-drive, and the Setup Status area appears:
Eventually the Horiba Jobin Yvon USB Installer window appears:
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h
Click the Next > button.
The End User License Agreement area appears:
i
Click the I accept this EULA radio button, then click the
Next > button.
j
A Software Installation warning window may appear:
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k
Click the Continue Anyway button.
The Installing the software for your HJY USB device... area appears.
When complete, the Congratulations! You are finished installing your HJY USB device. area appears:
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l
Click the Finish button.
The Horiba Jobin Yvon USB Installer window closes. The
InstallShield Wizard Complete area appears.
m Click the Finish button.
Installation of FluorEssence™ is complete.
n
Plug in all HORIBA software keys. Remove the
FluorEssence™ CD-ROM from the host computer.
5 Start FluorEssence™.
a On the desktop, double-click the
FluorEssence V3.5 icon.
The Fluorescence window appears:
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6 Choose a hardware configuration to run.
a Choose Collect.
A drop-down menu appears.
b
Choose Advanced Setup.
A drop-down menu appears.
c
Choose System
Configuration.
The Select Hardware Configuration window appears.
d
If Config list empty is
shown, click the New
button to create a new
instrument
configuration.
The System Configuration
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Wizard appears:
e
Choose one possible hardware configuration that your
system can run correctly. You may choose a radio button
for:
Load Factory Configuration The exact hardware setup
that HORIBA Scientific built for you.
Standard Instrument Configuration A basic hardware
configuration, for example, a typical FluoroMax®-4.
Detailed Component Configuration Your own hardware setup in which every component can be tailored.
Note: Most users do not choose the Detailed
Component Configuration.
f
Click the Next >> button.
If you chose Load Factory Configuration:
The Select a File window opens.
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g
Browse through the folders, and select the desired .jyc
file.
The InstallShield Wizard Complete window opens.
h
Continue with step 6 on page 21.
If you chose Standard Instrument Configuration:
The System Selection Page opens.
a
From the drop-down menus, choose the System Type
and Model. If your particular instrument includes
TCSPC, activate the TCSPC Enable checkbox.
b
Click the Next > button.
The Configuration Name page opens:
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c
d
Use the default name, or enter your own in the field.
Click the Next > button.
The Instrument Configuration page opens:
e
Choose the appropriate settings, leave the defaults, or
adjust as desired.
Click the checkboxes in the Available Accessories area to
activate all desired and available accessories.
Note: If you select a Sample Changer as an
accessory, a window appears asking which
Sample Changer: 2-position or 4-position. If
you select a Temperature Controller or Microscope as an accessory, a window appears asking for its details: be sure to choose
the correct Manufacturer.
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Note: Some parameters are not available for
certain systems (e.g., FluoroMax®), and thus
are grayed-out automatically.
f
Click the Next > button.
The Summary page opens.
g
Examine the Summary page to be sure that your
configuration is correct. To change the entries, click the
< Back button.
h
Click the Finish
button.
The Select Hardware
Configuration window
re-appears, with the
newly created hardware configuration in
the list.
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If you chose Detailed Component Configuration:
The Device Configuration screen opens.
a
Choose a component of your instrument to add from the
menu. In this case, a monochromator was selected.
b
Click the Next > button.
The Module type screen opens.
c
Choose the particular model of the component from the
menu.
d
Click the Next > button.
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The Communications Parameters screen opens.
e
f
Choose the parameters, or accept the default values.
g
Enter a name or description for the new component, or
use the default provided.
Click the Next > button.
The General information screen opens.
h
Click the Next > button.
The Accessory information screen opens:
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i
j
Choose the parameters, or accept the default values.
k
l
Review for correctness.
Click the Next > button.
The Summary screen appears.
Click the Validate Hardware button to verify that the
host computer communicates with the hardware.
m Click the Finish button.
The Device Configuration window reappears.
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n
Continue to add new components until the system
configuration is complete.
o
When complete, choose the Cancel button.
A Warning window
asks you to confirm
cancellation.
p
Click the Yes
button to stop
adding items to
the configuration.
The New Configuration
window appears.
q
Enter a brief name for
the hardware
configuration in the
ConfigID field.
r
Click the OK button.
The Select Hardware Configuration window re-appears, with
the newly created hardware
configuration in the list.
7 Choose the desired
hardware configuration, and click the OK
button.
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Loading correction-factor files
Correction-factor files adjust specific instruments for their optical
responses.
1 In the main FluorEssence window, choose
Collect.
A drop-down menu appears.
2 Choose Advanced Setup.
A drop-down menu appears.
3 Choose System Configuration.
4 If there is more
than one hardware
configuration available, the Select
Hardware Configuration menu appears.
Choose the desired
hardware configuration for the correction-factor file,
then click the Edit button.
The System Configuration window appears.
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5 Click the Preferences button.
6 Click the Instrument Correction Files icon.
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7 Choose the detector from its drop-down
menu, the monochromator from its dropdown menu, and the grating from its dropdown menu:
Click in each field to see the drop-down menu.
8 Browse for the appropriate correctionfactor file in the File field.
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9 If you need an extra row in the table for
additional combinations of detectors, monochromators, and gratings, click the Insert
button.
10Click the OK button when you are finished.
Note: You can have separate correction files for
different gratings on the same monochromator.
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2: Quick Guide to Running
a Scan
1 Turn on the host computer, and all instruments and accessories, as explained in
their respective instruction manuals.
2 Click on the FluorEssence shortcut to start FluorEssence™.
The FluorEssence window opens.
There are special buttons for running experiments in
FluorEssence™:
Experiment Menu
button
Choose an overall type of
experiment to run, such as
Phosphorescence, general Spectra
(e.g., emission or excitation), or
Single Point.
Previous
Experiment button
Modify slightly a previously set-up
experiment, and run it.
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Auto Run Previous
Experiment button
Run a previously set-up
experiment without modification.
Run JY Batch
Experiments button
Run an automated series of
experiments, including adjustable
repeats and delays between
experiments.
Open the Real Time Control
window directly, to adjust
experimental parameters in real
time.
With an existing graph selected,
create an .SPC file for use as an
overlay file. The existing graph
should contain a single spectrum.
Real Time Control
button
Make Overlay File
button
Create/Use
Calibration Curve
from CWA Data
button
3D Scan to 3D
Profile button
2D Intensity Map
button
From Single Point experiments,
create and use a calibration curve
for analytical measurements.
Extract excitation and emission
profiles from an excitationemission matrix. The active file
must be such a data matrix.
Create a two-dimensional intensity
map from microscope mapping
data.
Show Events button
The left E button reveals hardware
triggering events recorded during a
kinetics scan, for example, using a
hand-held pushbutton. A red line
appears at each event.
Hide Events button
The right E button hides red-linedenoted hardware triggering events
recorded during a kinetics scan.
Switch menu
between HJY
Software
Application and
Origin Std. button
Switches the menus at the top of
the main FluorEssence window
between FluorEssence™ and
Origin® functions.
Multigroup button
Close FluorEssence™ software,
and open Multigroup software.
Launch
DataStation button
Close the FluorEssence™
software, and start DataStation
software.
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Quantum Yield
calculator button
Overlay graph(s)
button
Opens the quantum-yield
calculator spreadsheet to calculate
the quantum yield of a sample and
chromaticity.
Overlays one plot on top of
another.
Blank Subtraction
button
Automatically subtracts a blank
(solvent) set of data from the
sample data.
Normalize Data
button
Automatically normalizes data to a
minimum intensity, a maximum
intensity, or a user-defined
constant.
Lets you see all the parameters for
the experiment in a single window.
View Experiment
Settings button
Extract Experiment
file from Data
(Notes) button
Extracts the experimental
parameters from Notes in a data
file, and creates an experiment file
from them.
Rescale Y button
Rescales the y-axis on a graph to
fit data on-scale.
Convert XYY data
to Contour Plot
button
Converts a table of data with
multiple y-columns into a contour
plot .
From many of these buttons, upon initial start-up of the software,
you can choose a hardware configuration and experiment type.
After a hardware configuration is loaded, each button has its own
separate function.
The Collect menu near the top of the
main window also has some of these
functions, plus another important
command, System UI Reset. In case
the six special buttons are grayed out,
choose the System UI Reset
command. See Chapter 7 for more
details.
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3 Click the Experiment Menu button.
The Select Hardware
Configuration window opens.
To force the appearance of
the Select Hardware
Configuration window:
a
Immediately upon
opening
FluorEssence™,
press the F8 key and
simultaneously click
the Experiment
Menu button
,
Or
b
At any time within FluorEssence™, press the F8 key
while choosing the Collect Menu / Advanced Setup /
System ReInitialization.
Note: This window does not appear if you have
only one hardware configuration installed. Skip to
page 34.
4 Choose a
system
configuration from
the list,
then click
the OK
button.
FluorEssence™ loads the chosen system configuration. The
Fluorescence Main Experiment Menu appears:
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5 Click an
available
scantype
button,
say,
Spectra.
The
Experiment Type window appears.
6 Choose an Experiment Type from
the menu, say,
Emission.
The parameters required for
an emission scan are loaded
into the Experiment Setup
window, which appears.
For an emission scan, the
default parameters are a
water-Raman scan.
7 Click the Next >>
button.
The Experiment Setup window opens:
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8 Click the Experiment File field, and enter a
new file name, or select a previously saved
file with the Load button.
9 Verify that the experimental parameters are
correct.
Be certain to check all parameters under all icons in the lefthand
column.
10Insert the sample into the sample compartment, and close the cover of the sample compartment.
11Click the Run button
The collected spectrum
is displayed on the
Intermediate Display
screen:
.
Note: If the scan is extremely fast, the Intermediate Display may be only
incompletely or rapidly
displayed before the
Origin window appears.
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You can watch the incoming data in real time, along with how the
positions of accessories vary. The scan may be paused, continued,
or aborted. After all data are recorded, the Intermediate Display
vanishes. For a new project, the Project name window appears:
12Enter a name for the entire project, or
browse for an existing project name with
the Browse button, then click the OK button.
All data are moved to Origin®’s workbook window:
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13Do post-processing as needed, using the
Analysis menu in the toolbar:
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3: FluorEssence™ Tips &
Tricks
Calibration of your instrument
Excitation calibration
Monochromator parameters for the xenon-lamp scan:
Monochromator Initial
Final
Increment Slits
(1200
wavelength wavelength
(bandpass)
grooves/mm)
Excitation
200 nm
600 nm
1 nm
1 nm
Emission
350 nm
--1 nm
Detector parameters for the xenon-lamp scan:
Detector (Signal) Integration time Units
Signal (S1)
100 ms
CPS
Reference (R1)
100 ms
mA
1 Close the sample compartment’s lid.
2 Start FluorEssence™.
3 In the main FluorEssence window, choose
the Experiment Menu button
.
The Fluorescence Main Experiment Menu appears.
4 Choose the
Spectra button.
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The Experiment Type window appears.
5 Choose Excitation, then click the
Next >> button.
The Experiment Setup
window for the xenon-lamp
scan automatically loads.
6 Click the Run button
.
The Intermediate Display opens. The xenon-lamp scan runs:
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Above is an uncalibrated FluoroMax® lamp-scan. The main peak
ought to be at 467 nm, but here appears near 480 nm.
7 Calibrate the excitation monochromator, if
required.
a
Double-click on the graph to un-embed it from the
workbook.
b
c
Expand the plot by clicking the Expand button
d
Click on the graph near the peak, to place the cursor on
the graph.
e
Using the left and right arrows on the keyboard, move the
cursor to the top of the peak.
f
Read the x-value of the plot: this is the wavelength of the
peak:
.
Click the cursor button to start the cursor function.
Note: Your lamp scan may appear different, depending on the
instrument and its configuration.
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Cursor
Wavelength
in nm
This example shows the peak at 477 nm, which is 10 nm
too high. Therefore we must calibrate the
monochromator.
g
Click the Previous Experiment button
.
The Experiment Setup window appears:
h
Click the RTC button.
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The Real Time Control window opens.
i
Click the Monos icon in the left column.
j
Enter the current, observed position of the peak in the
Position field (here, 477 nm).
k
Click the Calibrate Excitation 1 button.
The Calibrate window opens:
l
In the Peak of Interest field, enter the actual or expected
position of the peak (it ought to be 467 nm), then click the
OK button.
m At the bottom right of the Real Time Control window,
click the Cancel button.
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n
In the Experiment Setup window, click the Run button to
confirm the correct peak position. A correct scan is shown
below (peak is at 467 nm):
Cursor
Wavelength
in nm
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Emission calibration
Monochromator parameters for the water-Raman scan:
Monochromat Initial
Final wave- Increment Slits
or (1200
wavelength
(bandpa
grooves/mm) length
ss)
Excitation
350 nm
--5 nm
Emission
365 nm
450 nm
1 nm
5 nm
Detector parameters for the water-Raman scan:
Detector (Signal) Integration time Units
Signal (S1)
100 ms
CPS
Reference (R1)
100 ms
mA
Note: You can calibrate a T-side emission
monochromator in this way also.
1 Insert a cuvette with HPLC-grade, tripledistilled water in the sample compartment.
2 Close the sample compartment’s lid.
3 Start FluorEssence™.
4 In the main FluorEssence window, choose
the Experiment Menu button
.
The Fluorescence
Main Experiment
Menu appears.
5 Choose the
Spectra button.
The Experiment Type
window appears:
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6 Choose Emission,
then click the
Next >> button.
The Experiment Setup
window for an emission
scan appears, with the
water-Raman experiment
parameters automatically
loaded:
7 Click the Run button
.
The Intermediate Display opens. The water-Raman scan runs.
8 If the water-Raman scan is not at 397 nm,
calibrate the emission monochromator as
shown on pages 39–42.
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Using corrected signals
Introduction
Subtracting blanks, removing dark noise, and correcting for
inhomogeneities in the instrument or detector response give more
accurate spectra. Take special precautions to incorporate these
functions properly into a FluorEssence™ experiment. If S is
defined as the signal, correction follows the equation
Scorrected = (Smeasured – Sdark – Sblank) × Correction-factor file
Method
Any corrected signal (with a subscript “c”) or algebraic use of
corrected signals must explicitly include all desired corrected
signals in the Formulas list.
Corrected signals include:
Dark offset
Note: All desired corBlank subtraction
rections must be actiCorrection-factor file
vated in their respective checkboxes.
Example
The corrected signal, S1c, and corrected reference, R1c, along with
their ratio, S1c/R1c, all must be included in the Formulas list in
the Signal Algebra area. If unchecked, Sdark = 0, Sblank = 0, and
Correction-factor file = 1
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Projects and files
What is a project?
A project is a collection of workbooks of data, which hold:
Graphs (visual diagrams of the data)
Worksheets (tables of data)
Notes (comments about the data)
Project
Workbook
Graphs
Worksheets
Notes
Layers
Datasets
Axes
Backgrounds
Graphs themselves may contain multiple kinds of information,
including separate layers describing the data, the axes, the
background colors, etc.
Concerning worksheets, a dataset must contain at least two
columns, corresponding to x-y data pairs. Multiple y columns may
correspond to a single x column.
Note: For greater detail about projects, graphs, layers, and how to merge, combine, and separate them,
see the Origin® on-line help files.
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Combining two plots (datasets) into one graph
1 Make the first graph active.
2 Click the Overlay graphs button
.
The HJY_overlay window appears:
3 Click the Browse button
to browse for
the files to combine.
4 Activate the listview checkbox.
5 Select the desired graphs to combine.
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6 Click the >> button to add the desired
graphs to the combining list.
7 Click the OK button.
8 A window asks you to
choose which signal.
For example, in an excitation scan,
ratio the signal (S or T) to the
reference (R).
9 Click the OK button.
The window closes, and the Preview
updates with both graphs together.
10Click the OK button.
The overlaid graph appears.
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Merging two or more graph windows
This puts all the open layers on one single page.
1 Close all graph windows you don’t want to
merge.
2 Click the Merge button .
3 The Graph Manipulation merge_graph window appears:
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4 Click the Browse button
5
6
7
8
to browse for
the files to merge.
Activate the listview checkbox.
Select the desired graphs to merge.
Click the >> button to add the desired
graphs to the combining list.
Click the OK button.
The window closes, and the Preview updates with both graphs
together.
9 Click the OK button.
The merged graph appears:
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Splitting two graphs by extraction
This extracts each plot to a separate layer in the graph.
1 Click on the desired plot to activate it.
2 In the toolbar, choose the Extract to Layers
button
.
The Graph Manipulation layextract window appears:
3 Click the OK button.
The new graphs appear.
Note: Other buttons available using the Customize
Toolbar command are the button for splitting each
layer into a separate graph window, and the button for merging all open graph windows into one
graph. See the Origin® on-line help for more information.
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Saving and recalling a file
To save a project, when in a new, untitled project
Note: To determine if you are in an untitled, new
experiment, examine the path shown at the top of
the main FluorEssence window. It should show the
word “UNTITLED” at the end of the path.
1 Run an experiment.
When the experiment is complete, the Intermediate Display
disappears. The Project Name window appears.
2 Enter a
new name
for the project, or
browse for
an existing one.
Note: If you are using an existing project name, the
software will allow you to overwrite existing data, or
append the new data to the project.
3 Click the OK button.
The path of the project appears at the top of the main FluorEssence
window. The data are now saved.
To save data into a new project when another
project is already open
1 Run the experiment.
2 Choose File.
The File menu opens:
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3 Choose New....
The New window opens:
4 Choose Project from the list of
objects to create, then click the
OK button.
Note: Only a Graph or a Matrix lets you pick a
new Path and Name.
The data are now in an untitled project. Next you must create the
name for the file.
5 Choose File again.
The File menu opens.
6 Choose Save Project As....
The Save As window appears:
7 In the File name field, enter a name. In the
Save as type field, choose Project (*.opj)
from the list.
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8 Click the Save button.
Now the project has a new name.
To recall and open an existing project
1 Click File.
The File menu opens.
2 Choose Open....
The Open window appears:
3 Browse for the desired project, or examine
the Recent Projects list.
4 Click the Open button.
The project opens.
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4: Shutting Down
FluorEssence™
1 Save experiment files (and data files, if
created).
2 In the Experiment Setup window, click the
Close button
or the Cancel button
3 Close the FluorEssence
window, using either
the Close button ,
or, in the File dropdown menu, Exit.
.
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5: Multigroup Software
About Multigroup
Multigroup runs sequential and repeated fluorescence experiments.
Delays, temperature ramps, and multiple samples and wavelengthgroups are all included within Multigroup. You can sequentially
excite a sample with different wavelengths, then plot the emission
data on one view. This method is useful for energy-transfer studies,
and dual-wavelength experiments with fluorescent probes to
examine ion-transport.
An automated 2- or 4-position sample-changer is usually used with
Multigroup.
Below is a schematic of how the levels of multigroup looping and
repeat system can be set up, with two samples at two wavelengthgroups, plus a temperature-control accessory.
Repeat Time-Sequence Loops 1-2 (Time 0:00:00–0:01:30)
Repeat Time-Sequence 1 (40 seconds)
10 s delay
Repeat Time-Sequence 2 (40 seconds)
Sequential Accessory-Scan Loops 1-2 (Time 0:00:00–0:00:40)
Temperature-Control Point 1 (20 seconds)
Temperature-Control Point 2 (20 seconds)
Time-Sequence Interval Loops (Time 0:00:00–0:00:20)
Interval 1 (10 seconds)
Interval 2 (10 seconds)
Interleave Accessory Acquisition Interval 1 (Time 0:00:00–0:00:10)
sample 1,
wavelengthgroup 1
Acquire data
sample 1,
wavelengthgroup 2
Acquire data
sample 2,
wavelengthgroup 1
Acquire data
sample 2,
wavelengthgroup 2
Acquire data
Interval to
wait
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Requirements
To successfully install Multigroup, your host computer needs the
following:
Software
Windows® 2000, Windows® XP Pro, Windows® 7, or
Windows® Vista
Microsoft® .NET Framework 3.5.
Note: If the host computer does not have Mi-
crosoft® .NET Framework 3.5 installed, then
the Multigroup Installer attempts to access the
internet to download .NET Framework 3.5. If
there is no internet connection to the host
computer, contact HORIBA Scientific for advice.
Same version of FluorEssence™ (but 2.5.2 or higher) as
Multigroup
Hardware
Supports Windows® 2000, Windows® XP Pro, Windows® 7,
or Windows® Vista
1GB RAM
1 GB hard-disk space
One DVD-ROM drive
One available USB port
Video resolution of at least 1024 × 768
Usually an automated 2- or 4-position sample-changer
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Installation
1 From the Multigroup CD-ROM, run the installer.
If your host computer does not have Microsoft® .NET
Framework 3.5, the HJY Multigroup window appears.
2 Click the Yes button to download the software.
3 Follow the instructions on the Microsoft®
website for installing .NET Framework.
The program is large; the download and installation may take some
time.
4 Continue with the installer.
5 Click the
Next > button on the
HJY
Multigroup
window.
The Select
Installation
Folder area
appears:
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6 Leave the
default
folder, or
choose a
different
folder using the
Browse...
button.
7 Click the
Next >
button.
The Confirm
Installation
area appears.
8 Click the
Next >
button.
The Installing
HJY
Multigroup
area appears,
with a progress
bar.
When installation is complete, the Installation Complete area
appears:
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9 Click the
Close button to exit
the HJY
Multigroup
window.
An HJYMultigroup icon has been placed on
the computer’s desktop.
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Running Multigroup
1 Start the software in one of three ways.
a If you are working in FluorEssence™, save the project
file, then click the Multigroup button
FluorEssence™ toolbar.
in the
FluorEssence™ automatically closes.
or
b
On the desktop, double-click the
HJYMultigroup icon,
Be sure to exit from all Origin® or
FluorEssence™ software.
Note: FluorEssence™ and Origin® run in the
background as a shared resource while
Multigroup is active.
or
c
In the Windows® Start menu, choose All Programs.
A drop-down menu appears.
d
Choose Jobin Yvon.
A drop-down menu
appears.
e
Choose
HJYMultigroup.
The HJY Multigroup
window appears after
the splashscreen:
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2 Set up the experiment.
a In the System Configuration tab, choose the instrument
configuration from the drop-down menu.
b
Click the Connect Config button to connect to the
instrument.
Multigroup attempts to gain access to the desired instrument configuration in FluorEssence™. If unsuccessful, a
Devices Not Found window appears, asking you to emulate. Choose the Yes button if you want to emulate. If you
want to force Multigroup to emulate an instrument, activate the Force Emulate checkbox.
The instrument configuration automatically activates experimental parameters, which you can change manually.
c
In the Monochromators tab, activate the excitation and
emission monochromators’ checkboxes, if necessary.
d
In the Detectors tab, activate the desired detector
checkboxes, if necessary.
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To apply voltage to the detectors, click the Activate High
Voltage button.
e
In the Ratios tab, to record the corrected output using the
reference detector, activate the S/R button.
f
In the Time tab, enter an Integration Time, and choose
from the drop-down menu the units. Enter an Interval
Time and choose from the drop-down menu the units.
Enter a Total Time and choose from the drop-down menu
the units.
You also can adjust these parameters in the Time / Accessories tab.
g
In the Accessory Scan Settings tab, choose the
accessory for Sequential or Interleaving scans, using the
drop-down menus.
h
Click the Monos / Detectors tab to see the
monochromator and detector parameters.
i
Enter an excitation wavelength and emission wavelength
in row 1 of the Multigroup Mono Wavelengths table.
j
k
Add another row using the Add Grp button.
Change the Monochromator Parameters if necessary,
by entering a new value next to each parameter, or
choosing the parameter from a drop-down menu.
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l
Change the High Voltage or Data Units of each detector
by entering a new value if necessary.
m Click the Time / Accessories tab.
n
Enter the Interval Time and Sequence Duration, and
choose their units from the drop-down menus.
o
To add another sequence row, click the Add to List
button.
3 Run the experiment.
a Click the Acquire/View tab.
b
c
Click the Start button.
Use the various buttons to zoom in on and track the data
as they are recorded.
4 When finished, save the data via the Acquire/View tab:
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a
To export the data to Origin® and FluorEssence™, choose
Export to Origin.
A drop-down menu appears:
Select All Plots –
Current Data Set,
to save all the plots
on the graph.
b
Select Current
“Detector: S1” Data
Set to save only the
currently selected plot.
c
To copy the data to another program, choose Copy to
Clipboard.
A drop-down menu appears:
d
Select All Plots
– Current Data
Set, to save all
the plots on the
graph.
e
Select Current
“Detector: S” Data Set to save only the currently
selected plot.
f
To save the data in a file, choose Save to File, and select
the appropriate data-set.
g
Select All Plots – Current Data Set, to save all the plots
on the graph.
h
Select
Current
“Detector:
S1” Data
Set to save
only the
currently selected plot.
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Working with experiments and data
You can save existing experimental parameters (an “experiment”)
as well as data (“experimental results) to recall later for future use
and reference. An “experiment” file contains only the
experimental parameters, but no results. A “results” file contains
both experimental parameters and data recorded.
To save an experiment
1 Set up experimental parameters.
2 Click the Multigroup button.
A drop-down menu appears.
3 Choose Save
Experiment.
The Save Multigroup
Experiment Files
window appears.
4 Enter a file
name and
browse for the
desired folder.
5 Click the Save button.
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To recall an existing experiment
1 Click the Multigroup button
.
A drop-down menu appears.
2 Choose Open Experiment.
The Open Multigroup Experiment File window appears.
You may also
choose an
experiment
from the
Recent
Experiments
Files list.
3 Browse
for the desired file
and click
the Open button.
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To recall existing data (experimental results)
1 Click the Multigroup button
.
A drop-down menu appears.
2 Choose Open Results.
The Open Multigroup Result File window appears.
You may also choose
data from the Recent
Result Files list.
3 Browse for the
desired data,
and click the
Open button.
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Interleaved and sequential data
The choice of interleaved or sequential data is possible in
Multigroup. Imagine an experiment over time examining a pair of
wavelengths, λ1 and λ2.
Sequential data-acquisition
Sequential data-acquisition compares the first two data-points, then
the next two, then the next two, and so on. Direct comparison
between one λ1 and the next λ1 is only possible over a time interval
Δt:
Time Wavelength 1
t1
λ1
Δt
t2
t3
λ1
Δt
t4
Wavelength 2
λ2
Sequential method
λ2
Interleaved data-acquisition
Interleaved data-acquisition compares the first two data-points,
then the second with the third, then the third with the fourth, and so
on. Each wavelength measurement is used twice, once with the one
before it, and again with the one after it. Comparison between one
λ1 and the next λ1 is possible over a time interval ½Δt, half that of
sequential data-acquisition (see table below). This technique is
better for weak fluorescence or quicker analyses.
Time
t1
t2
t3
t4
Wavelength 1
λ1
½Δt
½Δt
½Δt
λ1
Wavelength 2
λ2
Interleaved method
λ2
Choose the accessory, and whether its method of data-acquisition
is Sequential or Interleave, in the Accessory Scan Settings
tab.
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6: Un-Installation
FluorEssence™
1 Close FluorEssence™.
2 Click the Start button to open the Start
menu.
3 There are two ways to continue:
a Choose Set Program Access and Defaults, or...
b Choose Control Panel.
The Control Panel opens:
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Click Add or Remove Programs.
4 In both cases, continue here.
The Add or Remove Programs window opens.
5 Click the Change or Remove Programs
icon.
A list of currently installed programs on the host computer
appears:
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6 Click HJY Application Software 3.5, which
becomes active:
7 Click the Remove button.
8 Follow the instructions to remove
FluorEssence™.
9 You may need to reboot the host computer.
FluorEssence™ is removed from the host computer.
10Remove the USB key from the USB port.
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Multigroup
1 Close Multigroup.
2 Click the Start button to open the Start
menu.
3 There are two ways to continue:
a Choose Set Program Access and Defaults, or...
b Choose Control Panel.
The Control Panel opens:
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Click Add or Remove Programs.
4 In both cases, continue here.
The Add or Remove Programs window opens.
5 Click the Change or Remove Programs
icon.
A list of currently installed programs on the host computer
appears:
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6 Click HJY Multigroup, which becomes active:
7 Click the Remove button.
8 Follow the instructions to remove
Multigroup.
9 You may need to reboot the host computer.
Multigroup is removed from the host computer.
10Remove the USB key from the USB port.
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7: FluorEssence™
Troubleshooting &
Technical Support
Troubleshooting
If the special buttons are gray,
1 Choose Collect.
A drop-down menu appears.
2 Choose System UI Reset.
The twelve buttons should become active again.
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On-line help files
Access from the Windows® Start menu:
1 Click the Windows® Start button.
A drop-down menu appears.
2 Choose All Programs.
From the drop-down list, select the Jobin Yvon group, then
FluorEssence Help.
The on-line help files appear:
Resize the window to your liking.
Access from the Experiment Setup or Real Time
Control window:
1 Click the Help button
or the F1 key.
Context-sensitive on-line help files appear. Resize the window to
your liking.
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Frequently-asked questions about
FluorEssence™
Many frequently-asked questions (FAQs) about FluorEssence™
may be found on the HORIBA Scientific website.
1 In the FluorEssence toolbar, choose Help.
A drop-down menu appears.
2 Choose FluorEssence FAQs.
If your computer is connected to the internet, your web browser
automatically opens in the FluorEssence™ software webpage:
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Video tutorials
For some common procedures, video tutorials are available to
guide you. The videos are .avi files, which can be played by
software such as RealPlayer®, Windows Media Player, etc.
Access to video tutorials
1 Click the Windows® Start button.
The Start menu appears.
2 Choose All Programs.
3 Choose the Jobin Yvon group.
4 Choose the Video Tutorials subgroup.
5 Click on the desired tutorial.
The tutorial opens in your chosen video-playing software.
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If you have a technical problem,
1 Please consult the FluorEssence™ help
files and this User’s Guide, as well as all
other manuals supplied with the system.
If you are unable to solve the problem,
2 Note the problem and any accompanying
error messages.
3 Determine FluorEssence™’s version number.
a
b
Choose the Help menu.
Choose About FluorEssence....
The About
FluorEssence window opens. Near the
bottom are the
FluorEssence™ and
Origin® version
numbers.
c
Click the View System Info button.
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The Installed Components window appears, displaying all the
software required for FluorEssence™.
d
Record the information by clicking the:
Save To File... button, which saves the information to a
file;
Zip Info button, which compresses the information while
saving it;
Print Info button, which prints out the software information.
e
Click the OK button to close the Installed Components
window.
f
Click the OK button to close the About FluorEssence
window.
4 Write down the software’s version numbers, along with the purchase dates, model
numbers, system configuration, and serial
numbers of the instrument and its accessories.
5 Please contact a HORIBA Scientific Service Department listed below.
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Be prepared to describe the malfunction and the attempts, if
any, to correct it. Note any error messages observed, and
have any relevant spectra (sample, polarization ratio, xenon-lamp
scan, water Raman scan, etc.) and system information
ready for us to assist you.
Contact information
Via the internet:
World-Wide Web
E-mail
www.horiba.com/scientific
service.jyus@horiba.com
In North America:
Telephone
Fax
1-877-546-7422
1-732-494-8796
In France:
Telephone
Fax
+33 (0) 1 64 54 13 00
+33 (0) 1 69 09 93 19
Worldwide:
China
Germany
Italy
Japan
UK
+86 (0) 10 6849 2216
+49 (0) 89 462317-15
+39 (0) 2 57603050
+81 (0) 3 58230141
+44 (0) 20 8204 8142
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8: Index
Key to the entries:
Times Roman subject or
font
keyword
Arial font
command, menu
choice, or dataentry field
Arial
Condensed
Bold font
window
Courier
New font
file name or
expression
<
< Back button ............................. 20
>
>> button .............................. 48, 50
.
.avi files ................................... 79
.NET Framework 3.5 ............ 57–58
.SPC file .................................... 30
A
About FluorEssence window 80–81
About FluorEssence... ............. 80
accessories ............................ 29, 35
Accessory information screen..... 22
Accessory Scan Settings tab . 63,
69
Acquire/View tab ....................... 64
Activate High Voltage button ... 63
Add or Remove Programs 71, 74
Add or Remove Programs window
......................................... 71, 74
Add to List button ...................... 64
Advanced Setup ........... 16, 25, 32
All Plots – Current Data Set ... 65
All Programs ................. 61, 77, 79
Analysis menu ........................... 36
Autorun................................... 9, 10
Auto Run Previous Experiment
button ..................................... 30
Available Accessories area...... 19
B
blank ..................................... 31, 45
blank subtraction ........................ 45
Blank Subtraction button ......... 31
Browse button ......... 35, 47, 50, 59
2
2D Intensity Map button ........... 30
3
3D Scan to 3D Profile button ... 30
C
Calibrate window ....................... 41
calibration ............................. 37, 43
calibration curve ......................... 30
Cancel button ...................... 24, 55
CD-ROM drive ............................. 9
Change button ........................... 11
Change or Remove Programs
icon .................................. 71, 74
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Choose Destination Location
area ......................................... 11
chromaticity ................................ 31
Close button ......................... 55, 60
Collect menu ...... 16, 25 ,76, 31, 32
combining two plots.................... 47
Communications Parameters
screen ..................................... 22
Company Name........................ 11
Config list empty ....................... 16
ConfigID field............................. 24
Configuration Name page ........... 18
Confirm Installation area ........... 59
Congratulations! You are
finished installing your HJY
USB device. area .................. 14
Connect Config button .............. 62
contact information ..................... 82
Continue Anyway button .......... 14
contour plot ................................. 31
Control Panel ...................... 70, 73
Convert XYY data to Contour
Plot button ............................. 31
Copy to Clipboard .................... 65
corrected signals ......................... 45
correction-factor file ....... 25, 27, 45
Create/Use Calibration Curve
from CWA Data button ........ 30
Current “Detector: S1” Data Set
............................................... 65
cursor .......................................... 39
Customer Information area ...... 10
cuvette ......................................... 43
D
dark noise .................................... 45
dark offset ................................... 45
Data Units .................................. 64
DataStation software ................... 30
Detailed Component
Configuration radio button .. 17,
21
detector ... 27–28, 37, 43, 45, 62–64
Detectors tab ............................. 62
Device Configuration window.... 21,
23
Devices Not Found window ........ 62
disclaimer ..................................... 5
DVD-ROM drive .......................... 8
E
Edit button .................................. 25
Emission .............................. 33, 44
emulate ....................................... 62
End User License Agreement
area ........................................ 13
Excitation................................... 38
excitation and emission profiles . 30
excitation-emission matrix ......... 30
Exit ............................................. 55
Expand button ........................... 39
Experiment File field ................ 34
Experiment Menu button... 29, 32,
37, 43
Experiment Setup window .. 33, 38,
40, 42, 44, 55, 77
Experiment Type window 33, 38, 43
export the data ............................ 65
Export to Origin ........................ 65
Extract Experiment file from
Data (Notes) button ............. 31
Extract to Layers button ........... 51
F
F1 key ........................................ 77
F8 key ........................................ 32
FAQs .......................................... 78
File field ......................... 27, 52–54
File menu.............................. 52–55
File name field .......................... 53
Finish button .................. 15, 20, 23
Fluorescence window ................ 15
Fluorescence Main Experiment
Menu .......................... 32, 37, 43
FluorEssence Help .................. 77
FluorEssence short-cut ............ 29
FluorEssence V3.5 icon .......... 15
FluorEssence window ... 25, 29–30,
37, 43, 52, 55
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FluorEssence™ CD-ROM ...... 9, 15
Force Emulate checkbox .......... 62
Formulas list .............................. 45
frequently-asked questions.......... 78
G
General information window ...... 22
Graph Manipulation layextract
window................................... 51
Graph Manipulation merge_graph
window................................... 49
grating ................................... 27–28
H
hard-disk space ....................... 8, 57
hardware configuration .. 16, 17, 20,
24–25, 31
hardware triggering ..................... 30
Help menu .................................. 78
Help button ................................. 77
help files................................ 77, 80
Hide Events button.................... 30
High Voltage.............................. 64
HJY Application Software 3.5 .... 72
HJY Multigroup .......................... 75
HJY Multigroup area.................. 59
HJY Multigroup window . 58, 60–61
HJYMultigroup .......................... 61
HJYMultigroup icon ............ 60–61
HJY_overlay window .................. 47
Horiba Jobin Yvon USB Installer
window............................. 12, 15
host computer .. 8, 9, 15, 23, 29, 71–
72, 74–75
Installing the software for your
HJY USB device... area ....... 14
InstallShield Wizard Complete
area ........................................ 15
InstallShield Wizard Complete
window .................................. 18
InstallShield® Wizard ............... 10
instrument configuration ............ 62
Instrument Configuration page ... 19
Instrument Correction Files icon
............................................... 26
integration time..................... 37, 43
Integration Time ....................... 63
Interleave .................................. 69
interleaved data-acquisition ........ 69
Interleaving ............................... 63
Intermediate Display 34–35, 38, 44,
52
Interval Time ....................... 63–64
J
Jobin Yvon group .......... 61, 77, 79
K
kinetics scan ............................... 30
L
Launch DataStation button ...... 30
License Agreement.................. 10
listview checkbox ................. 47, 50
Load button ................................ 34
Load Factory Configuration
radio button .......................... 17
I
M
I accept this EULA radio button13
increment .............................. 37, 43
Install button ............................... 12
Installation Complete area........ 59
Installed Components window ... 81
Make Overlay File button ......... 30
mapping data .............................. 30
Material Safety Data Sheets ......... 5
Merge button ............................. 49
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merging two or more graph
windows ................................. 49
microscope .................................. 30
Model ......................................... 18
Module type screen ................... 21
monochromator . 21, 27–28, 37, 39–
40, 43–44, 62–63
Monochromator Parameters ... 63
Monochromators tab................. 62
Monos / Detectors tab .............. 63
MSDS ........................................... 5
Multigroup button .... 30, 61, 66–68
Multigroup CD-ROM ................. 58
Multigroup Mono Wavelengths
table ........................................ 63
Multigroup software ................... 30
My Computer icon....................... 9
My Computer window ................... 9
N
New button ................................. 16
New Configuration window......... 24
New window ............................... 53
New... ......................................... 53
Next > button..... 10, 11, 13, 18, 19,
20, 21, 22, 23, 58, 59
Next >> button ......... 17, 33, 38, 44
Normalize Data button .............. 31
O
OK button .... 24, 28, 32, 35, 41, 48,
50–53, 81
Open button ................... 54, 67–68
Open Experiment ..................... 67
Open Multigroup Experiment File
window................................... 67
Open Multigroup Result File
window................................... 68
Open Results ............................ 68
Open window .............................. 54
Open... ....................................... 54
Origin® ........ 2, 4, 30, 35, 61, 65, 80
Overlay graph(s) button ..... 31, 47
P
Peak of Interest field ................ 41
Phosphorescence .................... 29
Position field ............................. 41
Preferences button ................... 26
Preview ................................ 48, 50
Previous Experiment button ... 29,
40
Print Info button ......................... 81
project ....................... 35, 46, 52–54
Project ....................................... 53
Project (*.opj) ......................... 53
Project name window .......... 35, 52
Project Name window ....................
Q
quantum yield ............................. 31
Quantum Yield calculator button
............................................... 31
R
R ............................................... 48
R1...............................................
......................................... 37, 43
R1c ............................................. 45
RAM ....................................... 8, 57
Ratios tab ................................... 63
Ready to Install the Program
area ........................................ 11
Real Time Control button ......... 30
Real Time Control window.. 30, 41,
77
RealPlayer® ................................ 79
Recent Experiments Files list . 67
Recent Projects list .................. 54
Recent Result Files list............ 68
Remove button .................... 72, 75
Rescale Y button ...................... 31
RTC button................................. 40
Run button ............... 34, 38, 42, 44
Run JY Batch Experiments
button ..................................... 30
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FluorEssence 3.5 User’s Guide rev. C (26 Jan 2012)
S
S ............................................... 48
S1 ......................................... 37, 43
S1c ............................................. 45
S1c/R1c ..................................... 45
S/R button .................................. 63
sample-changer ..................... 56–57
sample compartment ....... 34, 37, 43
Save as type field ..................... 53
Save As window.......................... 53
Save button .......................... 54, 66
Save Experiment ...................... 66
Save Multigroup Experiment Files
window................................... 66
Save Project As... ..................... 53
Save to File ............................... 65
Save To File... button................ 81
Select a File window .................. 17
Select Hardware Configuration
menu....................................... 25
Select Hardware Configuration
window................. 16, 20, 24, 32
Select Installation Folder area . 58
Sequence Duration .................. 64
Sequential ........................... 63, 69
sequential data-acquisition .......... 69
Service Department..................... 81
Set Program Access and
Defaults ........................... 70, 73
Setup Status area ...................... 12
Setup.exe icon............................. 9
Show Events button .................. 30
shutting down FluorEssence™ ... 55
Signal Algebra area ................... 45
Single Point ......................... 29–30
slits ........................................ 37, 43
Software Installation warning
window................................... 13
special buttons................. 29, 31, 76
Spectra button ......... 29, 33, 37, 43
splitting two graphs ..................... 51
Standard Instrument
Configuration radio button . 17,
18
Start button ......... 64, 70, 73, 77, 79
Start menu .......... 61, 70, 73, 77, 79
Summary page ............................ 20
Summary window....................... 23
Switch menu between HJY
Software Application and
Origin Std. button ................. 30
System Configuration ........ 16, 25
System Configuration tab ........ 62
System Configuration window ... 25
System Configuration Wizard ..... 17
System Selection Page .............. 18
System ReInitialization ............ 32
System Type ............................. 18
System UI Reset ................ 31, 76
T
T ............................................... 48
TCSPC ........................................ 18
TCSPC Enable checkbox ......... 18
Time / Accessories tab ...... 63–64
Time tab ..................................... 63
toolbar............................. 36, 61, 78
Tools menu ................................ 80
Total Time ................................. 63
troubleshooting ........................... 76
U
units ...................................... 37, 43
USB port ..................... 8, 57, 72, 75
USB software key ............. 9, 72, 75
User Name ................................ 11
V
Validate Hardware button ........ 23
version number ........................... 80
video resolution ...................... 8, 57
video tutorials ............................. 79
Video Tutorials subgroup.......... 79
View Experiment Settings button
............................................... 31
View System Info button .......... 80
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FluorEssence 3.5 User’s Guide rev. C (26 Jan 2012)
W
Warning window ......................... 24
water-Raman scan ..... 33, 43–44, 82
Windows Media Player ............... 79
Windows® ..... 2, 4, 8, 57, 61, 77, 79
workbook .............................. 35, 46
Y
Yes button ...................... 24, 58, 62
Z
Zip Info button ........................... 81
88