GeNei™ Imaging System

High Performance
UV Transilluminators
Instruction Manual
UVP, LLC
2066 W 11th Street, Upland, CA 91786
Tel: (800) 452-6788 / (909) 946-3197
Fax: (909) 946-3597
Ultra-Violet Products Ltd.
Unit 1,Trinity Hall Farm Estate,
Nuffield Road Cambridge CB4 1TG UK
Tel: +44(0)1223-420022 / Fax: +44(0)1223-420561
Web site: www.uvp.com
81-0278-01 Rev B
High Performance UV Transilluminators
Page 2
Introduction
The UVP Transilluminator offers the researcher uniform and intense sources of ultraviolet light
(radiation) in a compact package. The special design emits high intensity excitation UV wavelength
for back-illumination of transparent fluorescent materials. The 302nm UV back-illumination provides
a highly sensitive method to detect double-stranded nucleic acids that have been labeled with
fluorescent dyes such as ethidium bromide or acridine orange. Single stranded nucleic acids may
be detected, but with a lower excitation wavelength more sensitive for nucleic acid visualization
than the 365nm model.
High Performance UV Transilluminators are fitted with the exclusive 25 watt UV tubes. The
transilluminator is uniquely designed with increased UV intensity and uniformity, instant on
capabilities, no lamp flicker and reduced electrical consumption. Select models include variable
intensity with High, Medium and Low settings. The electronic ballast has a universal voltage
capabilities.
NOTE: Though the manual refers to the midrange UV waveband as 302nm, others refer to this
region as 300nm or 312nm. The spectral output of all these regions is the same.
A Word of Caution: UV Transilluminators are powerful sources of UV radiation that will cause
damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area
are properly protected. If not using the transilluminator with an imaging system darkroom, a UV
Blocking Cover should be attached to the transilluminator. Even though this cover blocks the
ultraviolet radiation emitted by the unit, UV Blocking Eyewear should be worn as well.
UVP UV Transilluminator features:
UVP’s long life filters are
available in a variety of
filter sizes from 15 x
15cm to 25 x 26cm
UV Blocking Cover is
included with the
transilluminator. The cover
is hinged and adjustable to
varying heights
Models include a control
knob for selection of
wavelength or intensity
Power switch
High Performance UV Transilluminators
Page 3
Specifications
Single UV High Performance Transilluminators
Single UV High Performance Transilluminators are designed with variable intensity settings.
Units are equipped with an electronic ballast and 25 watt tubes.
Width: 19.13” (486mm) Depth: 13.25” (336mm) Height: 5.63” (143mm) Height includes cover
The following models have 302nm midrange UV:
Part
Wave-
No. of
Intensity
Model
Number
length
Volts/Hz
Filter Size
Tubes
Style
TFM-20V
TFM-20V
TFM-26V
TFM-26V
TFM-30V
TFM-30V
TFM-40V
TFM-40V
95-0423-01
95-0423-02
95-0422-01
95-0422-02
95-0424-01
95-0424-02
95-0421-01
95-0421-02
302nm
302nm
302nm
302nm
302nm
302nm
302nm
302nm
100-115V/60Hz
230V/50Hz
100-115V/60Hz
230V/50Hz
100-115V/60Hz
230V/50Hz
100-115V/60Hz
230V/50Hz
20 x 20 cm
20 x 20 cm
21 x 26 cm
21 x 26 cm
25 x 30 cm
25 x 30 cm
20 x 40 cm
20 x 40 cm
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
Variable
Variable
Variable
Variable
Variable
Variable
Variable
Variable
20 x 40 cm
20 x 40 cm
4 x 25W
4 x 25W
Variable
Variable
No. of
Intensity
Style
The following models have 365nm longwave UV:
TFL-40V
TFL-40V
95-0420-01
95-0420-02
365nm
365nm
100-115V/60Hz
230V/50Hz
The following models have 254nm shortwave UV:
Part
Wave-
Model
Number
length
Volts/Hz
Filter Size
Tubes
TFS-20V
TFS-20V
TFS-26V
TFS-26V
TFS-30V
TFS-30V
TFS-40V
TFS-40V
95-0427-01
95-0427-02
95-0428-01
95-0428-02
95-0429-01
95-0429-02
95-0430-01
95-0430-02
254nm
254nm
254nm
254nm
254nm
254nm
254nm
254nm
100-115V/60Hz
230V/50Hz
100-115V/60Hz
230V/50Hz
100-115V/60Hz
230V/50Hz
100-115V/60Hz
230V/50Hz
20 x 20 cm
20 x 20 cm
21 x 26 cm
21 x 26 cm
25 x 30 cm
25 x 30 cm
20 x 40 cm
20 x 40 cm
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
Variable
Variable
Variable
Variable
Variable
Variable
Variable
Variable
High Performance UV Transilluminators
Page 4
2UV High Performance Transilluminators
Units are equipped with an electronic ballast and 25 watt tubes. Four tubes of each wavelength are
installed. These models have a single intensity. The physical dimensions of all models are:
Width: 14” x Depth: 11” x Height: 4.8” (356 x 279 x 122mm)
Model
Part
Number
Wavelength
TFML-20
TFML-20
TFML-26
TFML-26
TFML-30
TFML-30
TFML-40
TFML-40
95-0431-01
95-0431-02
95-0425-01
95-0425-02
95-0432-01
95-0432-02
95-0436-01
95-0436-02
302/365nm
302/365nm
302/365nm
302/365nm
302/365nm
302/365nm
302/365nm
302/365nm
Volts/Hz
Filter Size
No. of
Tubes
Intensity
Style
100-115V/60Hz
230V/50Hz
100-115V/60Hz
230V/50Hz
100-115V/60Hz
230V/50Hz
100-115V/60Hz
230V/50Hz
20 x 20 cm
20 x 20 cm
21 x 26 cm
21 x 26 cm
25 x 30 cm
25 x 30 cm
20 x 40 cm
20 x 40 cm
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
4 x 25W
Single
Single
Single
Single
Single
Single
Single
Single
Transilluminator Operation
Safety Precautions
When the UV Blocking Cover is not being
used, UV light may escape through the
holes dedicated to accepting the bracket
pins of the UV Blocking Cover.

Remove the black safety plugs from
their package

Insert the safety plugs through the
holes as shown.
Safety Plug
Set-Up

Place the transilluminator on a level work surface. Be sure that an air space exists
around the bottom of the work surface. This space allows for the proper air
circulation through the unit.

Plug the female end of the power cord into the transilluminator. For 230 volt
models, or those requiring special power cord connectors, ensure that the proper
configuration of male connector or plug is properly connected to the power cord.

Plug the male end of the power cord into a properly grounded electrical outlet. The
proper voltage of the transilluminator is found on the product information label. If
using the transilluminator with an imaging system, a jumper cable is required for
connecting to the darkroom. Refer to the imaging system documentation for
additional instructions.

The transilluminator may be equipped with a UV Blocking Cover. Remove the
brown protective paper from the cover. Insert the bracket pins on the cover into the
High Performance UV Transilluminators
Page 5
holes on the front of the transilluminator. The cover is adjustable to varying angles
for access to the filter surface.
If you are not using the transilluminator with an imaging system darkroom, do not
operate the unit without securing the cover. If the cover is missing, a UV Blocking
Faceshield must be worn to avoid UV exposure to the skin. UV Blocking Eyewear
should be worn even with the cover in place to avoid accidental UV exposure.
Using the Transilluminator
Place gel/sample on the filter area. It is recommended to place the gels on a Gel-Tray to
protect the filter surface from cuts and scratches. It is recommended that gloves be worn
to avoid contact with gel and staining agents.
Press the ON/OFF switch to ON. The UV tubes within the unit should be glowing beneath
the filter. If using the transilluminator with an imaging system, the system’s main power is
required to be in the ON position.
When using a transilluminator with multiple UV wavelengths, dial the knob to the
appropriate wavelength setting.
When using the Variable Intensity models, use the variable intensity settings as follows:



High: allows for UV excitation of fluorophores on gels for routine photography
and for excitation of gels with low sample concentration
Medium: Excellent for viewing and quick single-band excision
Low: Allows for positioning and preparation of gels, excising multiple bands and
focusing for photography
After viewing/photographing the sample, turn the transilluminator off.
Cleaning and Care of the Transilluminator
Clean unit surface with a damp soft cloth or sponge. Never use abrasive cleaners (can
damage the UV filter surface).
To protect the filter glass and minimize moisture and liquids on the glass, it is
recommended that you use a UV transmitting Gel-Tray. Refer to the Replacement Parts
for ordering information.
Replacing Tubes in the Transilluminator
1. Disconnect the transilluminator for the electrical supply.
2. Remove the filter cover: Use a Phillips head screwdriver to remove the four screws
on the sides of the unit. Lift the filter cover off the unit.
3. Remove the reflectors on the left and right side of the unit: Slide the reflectors up out
of the unit.
4. Remove the tube: Carefully rotate the tube and slide out of the socket. Replace with
a new tube by sliding the tube into the socket and rotating into place.
5. Insert the reflectors back into place and reattach the filter cover.
High Performance UV Transilluminators
Page 6
Maintenance/Repair/Technical Assistance
Replacement Parts/Accessories
For replacement parts or components not shown here, please call UVP Customer Service or place
of purchase. Please have the transilluminator model number available when you call.
Replacement Part Description
Part Number
Filter Assembly (TFM-20V, TFS-20V, TFML-20)
Filter Assembly (TFM-26V, TFS-26V, TFML-26)
Filter Assembly (TFM-30V, TFS-30V, TFML-30)
Filter Assembly (TFM-40V, TFS-40V, TFL-40V)
Filter Assembly (TFML-40)
Cover, UV blocking
Tube, 25 Watt, 302nm midrange UV
Tube, 25 Watt, 365nm longwave UV
Tube, 25 Watt, 254nm shortwave UV
Ballast, electronic universal voltage, 2UV models
Ballast, elect. universal voltage, variable intensity models
Fuse, 3.2A, 250V, Slo-Blo
Fuseholder, 5 x 20mm, RoHS
Switch
38-0190-02
38-0190-09
38-0190-11
38-0190-10
38-0190-04
19-0112-01
34-0072-01 – Qty 4
34-0060-01 – Qty 4
34-0073-01 – Qty 4
42-0068-02
42-0067-02
56-0002-05 – Qty 2
56-0023-02 – Qty 2
53-0126-03
Accessories Description
Gel-Cutter
Gel-Scooper
Gel-Tray, UV Transmitting, Sm. 11.5”W x 9”D (29 x 23cm)
Gel-Tray, UV Transmitting, Lg. 16.5”W x 10.5”D (42 x 27cm)
Gel-Tray, High Performance UV Transmitting (25 x 26cm)
Gel-Ruler, UV Fluorescing
Spectacles, UV Blocking
Goggles, UV Blocking
Faceshield, UV Blocking
Part Number
85-0002-01
85-0006-01
85-0007-01
85-0005-01
38-0296-03
85-0003-01
98-0002-01
98-0002-02
98-0002-04
High Performance UV Transilluminators
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Contacting Technical Support
UVP offers expert technical support on all of our products. If you have any questions about the product’s use,
operation or repair, please contact our offices at the locations below.
Note: A Returned Goods Authorization (RGA) number must be obtained from UVP’s Customer Service prior to
returning any product.
If you are in North America, South America,
East Asia or Australia:
If you are in Europe, Africa, the Middle East
or Western Asia:
Call (800) 452-6788 or (909) 946-3197, and ask
for Technical Support during regular business
days, between 7:00 am and 5:00 pm, PST.
Call +44(0) 1223-42002, and ask for
Customer Service during regular business
days between 8:30 am and 5:30 pm.
E-mail your message to: [email protected] or
[email protected]
E-mail your message to: [email protected]
Fax Technical Support at (909) 946-3597
Fax Customer Service at
+44(0) 1223-420561
th
Write to: UVP, LLC 2066 W. 11 Street, Upland,
CA 91786 USA
Write to: Ultra-Violet Products Ltd. Unit 1,
Trinity Hall Far Estate, Nuffield Road,
Cambridge CB4 1TG UK
Warranty
UVP, LLC warrants its Ultraviolet Transilluminators to be free of detects in materials and workmanship
for a period of two (2) years from date of purchase. The foregoing warranty of UVP shall be of no force
and effect if buyer has modified or damaged the product. Tubes and filters are warranted for 90 days.
All warranties or merchantability and fitness for any purpose and all other warranties, expressed or
implied, except those expressly set forth herein, are deemed waived and excluded.
UVP’s duty under the warranty is limited to replacement and/or repair of the defective part at the option
of UVP, LLC. UVP shall not be liable for any expenses or damages incurred by the purchaser except
as expressly set forth herein, and in no event shall UVP be liable for any special, incidental or
consequential damages of any kind. This warranty does not supersede any statutory rights that may be
available in certain countries.
UVP … Providing Quality Products for the Researcher Since 1932
From its start in 1932, UVP has become a story of growth and value fulfillment. Today, UVP services the needs
of science, industry and education throughout the world. A special significance of UVP research and
development arises from our constant communication with customers. UVP continually invites your comments
toward the improvement of UVP products or the development of custom types of ultraviolet light sources. UVP
will work with you through development and production of a product that meets your specific needs. From all of
us at UVP, Thank You.