Spectrophotometer User Guide

Spectrophotometer
User Guide
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269-220300, Rev. A
Contents
Introduction.........................................................................................1
Conventions used in this manual .............................................................. 2
Questions or concerns............................................................................... 2
Spectrophotometer Basics ....................................................................3
Keypad and LCD display .......................................................................... 3
Connectors ............................................................................................... 5
Sample holders.......................................................................................... 6
Software................................................................................................7
Local and Computer Control.................................................................... 8
Basic operation.......................................................................................... 8
Parameter entry......................................................................................... 9
Saving and renaming methods and data .................................................. 10
Saving a method .................................................................................. 10
Saving data........................................................................................... 11
Scan ....................................................................................................13
Scan Method screen ................................................................................ 14
Scan Graph function keys ....................................................................... 18
Manipulate menu options .................................................................... 18
Track Table screen............................................................................... 22
Peak Table screen................................................................................. 23
Ratio Table screen................................................................................ 23
Peak Height screen............................................................................... 24
Fixed...................................................................................................25
Fixed method parameters ........................................................................ 25
Fixed Results screen ................................................................................ 29
Quant .................................................................................................30
Quant method parameters ...................................................................... 30
Quant Standards screen........................................................................... 34
Quant Calibration screen ........................................................................ 35
Quant Results screen............................................................................... 36
Spectrophotometer User Guide
i
Rate ....................................................................................................37
Rate Method screen................................................................................. 38
Rate Graph screen ...................................................................................41
Manipulate menu options .................................................................... 42
Rate Results screen ..................................................................................46
Parallel Rate measurements using the Cell Changer.................................46
Multicomponent Analysis (MCA) ......................................................48
MCA Method screen............................................................................... 48
MCA Standards screen............................................................................ 51
MCA Wavelength screen.........................................................................52
MCA Calibration screen .........................................................................53
Analyzing a sample..................................................................................53
Bio Tests.............................................................................................55
BioMate applications...............................................................................55
Nucleic acid measurements .................................................................. 56
Direct UV measurements of nucleic acids ............................................... 59
Oligonucleotide measurement – calculated factor ................................ 61
Protein measurements – Standard Curve method....................................63
Direct UV measurements of proteins ......................................................65
Warburg-Christian test ........................................................................... 67
Running the Warburg-Christian test.................................................... 67
Cell growth test....................................................................................... 68
Measuring cell growth..........................................................................68
Oligo calculator functions .......................................................................69
Specifying a base sequence ...................................................................69
Using the oligonucleotide calculator..................................................... 69
AquaMate Methods ............................................................................70
How to run an AquaMate method ..........................................................71
Loading a method ................................................................................71
Saving a method to the Library ............................................................72
About the method results .....................................................................72
Disk 1 - Merck Spectroquant® methods...................................................73
Operation ............................................................................................ 73
Test results........................................................................................... 74
Disk 2 – Hach test kit methods...............................................................75
.FXD method files................................................................................ 75
.QNT method files .............................................................................. 77
Disk 3 – Dr. Lange cuvette and pipette test kit methods ......................... 80
Operation ............................................................................................ 80
Test results........................................................................................... 80
Disk 4 – CHEMetrics Vacu Vial methods .............................................. 82
Operation ............................................................................................ 82
ii
Spectrophotometer User Guide
Test results........................................................................................... 82
AquaMate method descriptions............................................................... 84
Merck .................................................................................................. 84
Hach.................................................................................................... 93
Lange ................................................................................................... 98
CHEMetrics ...................................................................................... 101
Library..............................................................................................103
Instrument Library screen ..................................................................... 103
Working with files stored in the instrument library............................ 105
USB Memory Device Library screen ..................................................... 106
Working with files stored on a USB Memory device .......................... 107
UVcalc ..............................................................................................108
Specification ......................................................................................... 109
Operation ............................................................................................. 109
Defining a formula............................................................................. 110
Setting up a Scan calculation.............................................................. 111
Setting up a Fixed calculation ............................................................ 113
Setting up a Quant calculation........................................................... 114
Modifying an equation by adding parameters .................................... 115
Modifying an equation by adding constants....................................... 116
UVcalc Error Messages.......................................................................... 117
Setup ................................................................................................119
Setup screen .......................................................................................... 119
Clock screen.......................................................................................... 120
Printer menu options ............................................................................ 120
Environment screen .............................................................................. 121
Language............................................................................................ 122
Sound ................................................................................................ 122
Date Format ...................................................................................... 122
Automatic Cal. Val. ........................................................................... 122
Default File Type............................................................................... 123
LIMS Support.................................................................................... 123
Use Sample IDs ................................................................................. 123
AutoSave Result ................................................................................. 124
AutoPrint Results............................................................................... 125
User Log-On...................................................................................... 125
History File........................................................................................ 127
Wavelength Calibration screen.............................................................. 128
Optical Initialization screen .................................................................. 129
White Light screen................................................................................ 130
Setup CVC screen................................................................................. 130
Lamps screen......................................................................................... 130
Spectrophotometer User Guide
iii
Cell Changer ....................................................................................132
Installing and removing the Cell Changer .............................................132
Installing the Cell Changer ................................................................ 132
Removing the Cell Changer ...............................................................133
Operating the Cell Changer ..................................................................133
SuperSipper......................................................................................136
Sipper screen .........................................................................................137
SuperSipper Calibration ........................................................................138
Sipper Calibration screen ...................................................................139
Calibrate Sipper screen.......................................................................139
MiniSipper .......................................................................................141
Sipper screen .........................................................................................142
MiniSipper Calibration .........................................................................143
Sipper Calibration screen ...................................................................143
Calibrate Sipper screen.......................................................................144
Calibration Verification Carousel.....................................................145
CVC Setup ........................................................................................... 145
CVC Setup screen..............................................................................146
Automatic CVC calibration ...............................................................147
Installing the CVC carousel ..................................................................147
CVC Home screen................................................................................148
Results screens....................................................................................... 149
Removing the CVC carousel .................................................................149
Analog Data Output.........................................................................150
Connection ........................................................................................... 150
Setup.....................................................................................................150
Maintenance.....................................................................................152
Error Codes........................................................................................... 153
Routine maintenance ............................................................................154
Cleaning the instrument exterior........................................................ 154
Removal and replacement of the tungsten-halogen lamp .......................155
Removal and replacement of the deuterium lamp..................................157
BioMate 6 Test Parameters...............................................................160
Calculations for BioMate 6 Tests......................................................165
BioMate Oligo Calculator ................................................................169
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Spectrophotometer User Guide
Introduction
This manual explains how to operate the following spectrophotometers:
•
Helios™ Zeta
•
UV-10
•
Helios™ Omega
•
AquaMate™ Vis
•
Evolution™ 160
•
AquaMate™ Plus UV-Vis
•
BioMate™ 6
All of these instruments can be run from the integral keypad and LCD
display, or from an external computer (additional software is required).
Each system is comprised of a spectrophotometer with integral keypad,
LCD display with adjustable contrast, and embedded Local Control
Software, plus two USB ports for connecting an external memory device
and printer.
Note
A USB memory device ships with each system.
The embedded Local Control software controls all aspects of the system’s
operation. You can collect data at fixed wavelengths, at all points in a
spectral range, at one location over a period of time, or run quantitative
experiments. The Local Control software includes our UVcalc application
which automatically calculates results from measurements using userdefined equations in Scan, Fixed and Quant modes.
Spectrophotometer User Guide
1
Conventions used in this manual
This manual includes safety precautions and other important information
presented in the following format:
Note
Notice
Caution
Notes contain helpful supplementary information.
Follow instructions labeled “Notice” to avoid damaging the system
hardware or losing data.
Indicates a hazardous situation which, if not avoided, could result in minor
or moderate injury.
Questions or concerns
In case of emergency, follow the procedures established by your facility. If
you have questions or concerns about safety or need assistance with
operation, repairs or replacement parts, you can contact our sales or service
representative in your area or visit our web site at
www.thermo.com/spectroscopy.
2
Spectrophotometer User Guide
Spectrophotometer Basics
This chapter describes the major components of your spectrophotometer.
Keypad and LCD display
To adjust the contrast for the LCD
display, press Home and then press the
left or right arrow key.
Keypad and LCD display
Button
Description
Function
Arrow keys
• Select an option on the current
screen or popup menu.
• From any graph with the Track
option selected, move the
crosshairs right or left.
• Move the Cell Changer.
• Change display contrast (from
Home or initialization screens
only).
Spectrophotometer User Guide
3
Button
Description
Function
Numeric keys
Enter a number, minus sign or
decimal point.
Function keys
Access and perform system
functions as indicated by
associated software labels.
Available functions depend on
screen in use.
ESC
• Delete entry.
• Remove pop-up box.
• Clear error message.
Enter
Accept changes to field or
parameter value.
Run
Measure sample according to
current method.
Home
Return to Home screen.
Zero/Base
For Scan methods, performs a
baseline scan.
For Fixed, Quant and Rate methods,
zeros the instrument.
Key functions
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Spectrophotometer User Guide
Connectors
2
1
4
3
Front panel features
1
Sample compartment
3
Keypad
2
LCD display
4
USB memory device port
1
2
3
4
5
Rear panel features
1
USB printer port
4
Power switch
2
RS-232 PC/LIMS port
5
Power connector
3
Connectors to control optional
accessories
Spectrophotometer User Guide
5
Sample holders
Variable pathlength cell holder (supplied with all
instrument models)
Holder for 1-inch square Hach® Cells and AccuVac®
Ampule (supplied with AquaMate models)
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Spectrophotometer User Guide
Test tube holder (supplied with AquaMate
models)
Software
The Local Control Software is organized in a tree structure with all
functions accessed initially from the Home screen. You can collect and
analyze data in five modes:
•
Scan – Measures absorbance at all points in a defined wavelength
range.
•
Fixed – Measures absorbance or % Transmittance at up to 20 fixed
wavelengths.
•
Quant – Determines sample concentration by comparing measured
absorbance values against a concentration curve.
•
Rate – Measures absorbance at one wavelength over a defined period of
time.
•
MCA – Quantifies up to 20 components in a sample mixture by
comparing measured absorbance values against the absorbance of
known standards.
The Scan, Fixed, Rate, Quant and MCA options available from the Home
screen are independent applications. Only one application can operate at a
time.
Notice
Note
Loading another application will overwrite any current data.
Your instrument may display a Home screen that lists purchased methods
or other individual methods that were selected manually for display at start
up. To display the default Home screen with the options listed above from
the start-up screen, choose General Tests.
Spectrophotometer User Guide
7
Local and Computer Control
After power up, the instrument is automatically set to local control. Follow
these steps to switch between local control and control from an external
computer.
Remote computer
To switch from local control to an external computer via the RS-232C
port:
1. Display the Home screen.
2. Wait until the instrument is idle.
3. Press Remote.
REMOTE
Local computer
To return to local control:
1. Wait until the instrument is idle.
2. Press Home.
The main menu is displayed and the embedded keypad is
operational.
Basic operation
To operate the Local Control software:
8
Spectrophotometer User Guide
•
Use the function keys directly below the LCD display to move between
software screens within an application.
•
To initiate an action, use the arrow keys to select an option on the
current screen or popup menu and then press Enter.
•
To return to the Home screen, press Home.
Parameter entry
The Local Control software provides the following types of screens and
menus for setting and editing parameters:
Pop-up entry box
Use to enter numerical values. The valid range for the parameter is
displayed in the menu. This example sets the starting wavelength for
scanning:
EDIT VALUE
START
:
400
MINIMUM
:
190.0
MAXIMUM :
1100.5
Use the numeric keypad to enter a new value and then press Enter. Press
ESC to close the menu without changing the parameter.
Pop-up menu
Use to select from a list of available options. This example defines the
level of smoothing applied to the collected data.
SMOOTHING
NONE
LOW
MEDIUM
HIGH
Use the arrow keys to highlight an option and then press Enter.
Toggle
Text entry screen
Alternates between two available settings (e.g., yes/no or on/off) when
you press Enter.
Use to enter alphanumeric characters such as the Test Name. The
software displays the available characters.
To enter a letter or symbol, use the arrow keys to select the character on
the display and press Enter. Numbers can be entered using the numeric
keypad.
The left arrow function key works as a backspace. To remove the entire
text string, press ESC.
Spectrophotometer User Guide
9
When the entry is complete, press Accept to input the new text or Cancel
to close the screen without changing the parameter.
The following function keys are available from the text entry screen.
Function key
Description
Cancel
Cancels the operation and returns the previous screen.
Accept
Accepts the text and returns to the previous screen.
Å
Clears the last character in the text string.
Saving and renaming methods and data
The Save screen appears in many places in the Local Control software. The
options available on the Save screen depend on the type of data being saved
(method or data).
Saving a method
To save a method, display the method parameters screen and press Save
Method. The Save screen is displayed:
* SAVE *
TYPE
FILENAME
SMART START
TEST NAME
DRIVE
CANCEL
M SCAN
.SCN
: NO
LIBRARY
SAVE
Save screen for saving methods
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Spectrophotometer User Guide
Item
Function
Type
This field is assigned by the software, depending on the type of
method being saved.
Filename
This field is selected automatically when you first enter the Save
screen. Use the simulated keyboard or the numeric keypad to enter
up to 8 characters for the filename and then press Accept. See Text
Entry screen for details.
Item
Function
Smart Start
Selects whether the file will be displayed on the start-up screen.
Press Enter to toggle the Smart Start setting between Yes and No.
When one or more files are selected for display on the start-up
screen, the start-up screen appears when the instrument is turned
on (instead of the default Home screen).
Press Home to see the new start-up screen.
From the start-up screen, press General Tests to display the default
Home screen.
Test Name
Use the simulated keyboard or the numeric keypad to enter a
descriptive name for the method and then press Accept. See Text
Entry screen for details.
Drive
Select a destination for the method file. Press Enter to toggle the
Drive setting between:
Library – saves the method in the instrument library.
USB Memory – saves the method on the Library USB memory
device installed in the USB Memory Device port on the front of the
instrument.
Function key
Description
Save
Stores your entries and displays the method parameters screen.
Cancel
Cancels the Save operation and displays the method parameter
screen.
Saving data
To save displayed data, press Save Data. The Save screen is displayed:
* SAVE *
TYPE
FILENAME
FILE TYPE
TEST NAME
DRIVE
CANCEL
D SCAN
.SCN
: NORMAL
LIBRARY
SAVE
Save screen for saving data
Item
Function
Type
This field is assigned by the software, depending on the type of data
being saved.
Spectrophotometer User Guide
11
Item
Function
Filename
This field is selected automatically when you first enter the Save
screen. Use the simulated keyboard or the numeric keypad to enter
up to 8 characters for the filename and then press Accept. See Text
Entry screen for details.
File Type
Selects a file format:
Normal – the native file type of the Local Control Software. This is
the only file type that can be saved in the instrument library.
CSV – Comma separated variable)
JCAMP-DX – JCAMP data exchange format.
Test Name
Use the simulated keyboard or the numeric keypad to enter a
descriptive name for the data and then press Accept. See Text
Entry screen or details.
Drive
Select a destination for the data file. Press Enter to toggle the Drive
setting between:
Library – saves the file in the instrument library. (Only Normal file
types are accepted.)
USB Memory – saves the file on the Library USB memory device
installed in the USB Memory Device port on the front of the
instrument. (Saves all file types.)
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Spectrophotometer User Guide
Function key
Description
Save
Stores your entries and displays the data screen.
Cancel
Cancels the Save operation and displays the data screen.
Scan
Select the Scan application on the Home screen to collect and analyze data
at all points in a defined wavelength range.
Use the Scan Method screen to set data collection and analysis parameters.
When you are finished setting parameters, press Zero/Base to perform a
baseline scan with the current method. When you are ready to analyze the
first sample, place the sample cell in the sample holder and press Run.
The spectrophotometer performs the scan and displays the result on the
Scan Graph screen. From there the spectrum can be manipulated and saved
to a Library or USB memory device.
Spectrophotometer User Guide
13
Scan Method screen
Use this screen to set instrument and analysis parameters for collecting and
analyzing spectra. To change a parameter setting, highlight the parameter
and press Enter. See Parameter Entry for more information.
* SCAN *
SCAN TYPE
TEST NAME
MODE
START
STOP
BANDWIDTH
SPEED
DATA INTERVAL
PEAK TABLE
GRAPH HIGH
GRAPH LOW
SMOOTHING
LAMP CHANGE
USER
UVCALC
UV CALC
RESULTS
Note
PRINT
METHOD
STANDARD
TEST 1
ABS
400.0 nm
600.0 nm
2.0 nm
1200 nm/min
1.0 nm
OFF
2.000
0.000
NONE
325 nm
USER 1
0
SAVE
METHOD
VIEW
GRAPH
VIEW
RESULTS
The current spectrum will be lost if you change any of the Scan method
parameters except the user name (User).
Parameter
Function
Scan Type
Sets scan speed and data interval.
Standard mode - Allows you to manually set the scan speed and data interval.
Intelliscan™ mode – Sets the data interval automatically and varies the scan speed according to the
absorption of the sample.
Test Name
14
Use the Text Entry screen to enter a descriptive name for the method. The Test Name is saved with the
method and any spectra produced by the method.
Spectrophotometer User Guide
Parameter
Function
Mode
Selects the format used to measure and display the collected spectrum.
ABS - Absorbance vs. Wavelength
%T - % Transmittance vs. Wavelength
I - Light Beam Intensity mode vs. Wavelength
1D - First derivative of the Absorbance vs. Wavelength spectrum
2D - Second derivative of the Absorbance vs. Wavelength spectrum
3D - Third derivative of the Absorbance vs. Wavelength spectrum
4D - Fourth derivative of the Absorbance vs. Wavelength spectrum
Start
Defines the starting wavelength of the scan (must be at least 4 nm less than the Stop wavelength). Enter
a wavelength between 190.0 nm and 1096.0 nm (or between 315 nm and 1096 nm for the AquaMate Vis).
If the start wavelength requires the deuterium lamp, the lamp will activate automatically.
Stop
Defines the ending wavelength of the scan (must be at least 4 nm greater than the Start wavelength).
Enter a wavelength between 190.0 nm and 1100.0 nm (or between 319 nm and 1100 nm for the
AquaMate Vis).
Bandwidth
This parameter is fixed at 2.0 nm.
Speed
Sets the scan speed. The available options depend on the setting for Scan Type (above).
If Scan Type = Intelliscan Mode, select from Color/Zip/Survey/Normal/Quant/Hi-Res.
If Scan Type = Standard Mode, select from 3800, 2400, 1200, 600, 240, 120, 30, 10 or 1 nm per minute.
Data interval
Sets the frequency of data points in the spectrum. The available options depend on the setting for Scan
Type (above).
If Scan Type = Intelliscan Mode, the data interval is defined by the Intelliscan Mode setting according to
the table below.
Intelliscan Mode Setting
Data Interval
Color
10 nm
Zip
4 nm
Survey
2 nm
Normal
1 nm
Quant
0.5 nm
Hi-Res
0.2 nm
Spectrophotometer User Guide
15
Parameter
Function
If Scan Type = Standard Mode, the allowable data interval is defined by the Standard Mode scan speed
setting according to the table below. .
Speed
Data interval
3800
10, 4
2400
10, 4, 2
1200
10, 4, 2
600
10, 4, 2, 1, 0.5
240
10, 4, 2, 1, 0.5, 0.2
120
10, 4, 2, 1, 0.5, 0.2
30
10, 4, 2, 1, 0.5, 0.2
10
10, 4, 2, 1, 0.5, 0.2
1
10, 4, 2, 1, 0.5, 0.2
Parameter
Function
Peak Table
Selects the type of peak/point picking done automatically as part of the method. Results are reported on
the Peaks screen. Peaks information is stored with any saved spectrum. Available options include:
Off - Sets Peak Table to Off. No peaks information is produced as part of the scan.
Peaks - Picks the highest peaks in a spectrum up to a maximum of 10 peaks.
Valleys - Picks the lowest valleys in a spectrum up to a maximum of 10 valleys.
Pks & Valleys - Picks the 5 highest peaks and the 5 lowest valleys.
Zero Cross - Picks all the points where the spectrum crosses zero up to a maximum of 10 crossing points.
Track - Allows the data values to be reported at up to 10 user selected wavelengths.
Ratio - Allows you to specify a ratio (λ1 / λ2) to be automatically calculated at the end of the scan.
Enter each wavelength at the prompt and press Enter.
Corr Ratio - Allows you to specify the ratio of two wavelengths to be calculated relative to a third
wavelength [(λ -λ3)/(λ2 -λ3)] at the end of a scan. Enter the wavelengths for the numerator and
denominator at the prompts and press Enter.
Peak Height - Allows the height of a peak to be calculated relative to a drawn baseline rather than y = 0.
Enter the Baseline 1, Peak, Baseline 2 wavelengths at the prompts and press Enter.
Note: After the wavelengths have been entered, go back to the Scan method screen and save the
method.
16
Spectrophotometer User Guide
Parameter
Function
Graph High
Sets the upper graph limits on the Scan Graph screen.
Select from range (Graph Low + 0.01) to 6.00. Graph High must be 0.01 greater than Graph Low.
Graph Low
Sets the lower graph limits on the Scan Graph screen.
Select from range -0.3 to (Graph High - 0.01). Graph Low must be 0.01 less than Graph High.
Smoothing
Applies No, Low, Medium or High modified/improved Savitzky-Golay smoothing to the spectrum.
Lamp Change
Selects the wavelength at which the source is changed between the tungsten (W) and deuterium (D2)
lamp. Select from 315, 320, 325, 330, 335, 340, D2, W.
Note: Selecting D2 or W manually overrides the Lamp Change setting and the selected lamp will be used
regardless of the wavelength set.
Note: This parameter is not available for the AquaMate Vis.
User
Use the Text Entry screen to enter a user name. The user name is saved with the method and any spectra
produced by the method.
Note: Changing the user name will not cause the current spectrum to be lost.
Note: If User Log-on is in operation, the user name cannot be changed.
UVcalc
Displays the UVcalc screen. See UVcalc for more information.
Function key
Description
View Results
Displays the Scan Peak Table screen after you perform a peak function or the Track Table screen after
you use Track.
View Graph
Displays the Scan Graph screen.
Save Method
Displays the Filename Function screen and then saves the method, including User Name, Test Name and
track wavelengths if Peak Table is set to Track.
Print Method
Prints the current method parameters using the selected printer.
UVcalc Results
Displays the UVcalc results screen if an equation has been entered and results are available.
Spectrophotometer User Guide
17
Scan Graph function keys
Function key
Description
View Results
Displays the Scan Peak Table screen.
Scan Page
Displays the Scan screen
Save Data
Displays the Save screen for saving methods and data to a USB
memory device.
Print Graph
Prints the displayed data using the selected printer.
Manipulate
Displays the Manipulate popup-menu (see descriptions below).
Scan Graph function keys
Press Run to start a scan using the current method.
Press Zero/Base to start a baseline using the current method.
Manipulate menu options
MANIPULATE
TRACK
RESCALE
COMPARE
MODE
PEAKS
SMOOTHING
ORIGINAL
18
Spectrophotometer User Guide
Menu Option
Function
Track
Reports x- and y-axis values selected with the tracking
cursor.
Rescale
Changes x- and y-axis scales automatically or
manually.
Compare
Loads a reference spectrum for comparison.
Mode
Defines the format of the collected and displayed data.
Select from %T / ABS / 1D / 2D / 3D / 4D.
Peaks
Finds spectral peaks. Select from Peaks/Valleys/Peaks
& Valleys/Zero Cross/Ratio/Corr. Ratio/Pk Height.
Smoothing
Applies Low, Medium or High modified/improved
Savitsky-Golay smoothing to the spectrum.
Original
Resets the graph to display the data as originally
collected.
Track
This option displays the tracking cursor (crosshairs) which can be used to
select up to 10 x-axis locations to be measured and reported.
To mark a wavelength, move the cursor to the desired location and press
Enter. The cursor always moves to a data point regardless of the displayed
scales.
Press View Table to see a table of measured values for the selected locations.
If you exit the Track graph, the markers will be deleted.
Track graph function keys
Function key
Description
View Table
Displays the Track Table which lists the measured value at each
selected wavelength.
Fast/Slow
Toggles between two cursor speeds. In Fast mode, the cursor
jumps 5% of the graph or to the next data point, whichever is
greater. In Slow mode, the cursor jumps to the next data point or
the next display pixel, whichever is greater. The function key
label shows the deselected speed; i.e., the opposite of the one
you are currently using.
Clear All
Deletes all the markers and the data from the Track Table.
Print Graph
Prints the displayed data (including markers and x- and y-axis
values) using the selected printer.
Scan Graph
Displays the Scan Graph screen.
Track screen function keys
Press ESC to delete markers in sequence, starting with the marker that has
the highest assigned number.
Rescale
RESCALE
AUTO
GRAPH HIGH
GRAPH LOW
GRAPH START
GRAPH STOP
PROCEED
Spectrophotometer User Guide
19
Menu Option
Function
Auto
Displays the Scan Graph screen with the x- and y-axes
rescaled so that the spectrum fills the screen.
Graph High
Pops up a window to enter the Graph High limit.
Graph Low
Pops up a window to enter the Graph Low limit.
Graph Start
Pops up a window to enter the required start
wavelength.
Graph Stop
Pops up a window to enter the required stop
wavelength.
Proceed
Used after Graph High, Graph Low, Graph Start or
Graph Stop to return to the Scan Graph screen with the
graph rescaled using the new parameters.
Compare
This option allows you to display a reference spectrum for comparison.
When selected, Compare goes to the Library screen and displays a list of
scan data files. Select a reference file and press Enter. The reference
spectrum appears as a dotted trace.
The reference spectrum remains on the screen (and is printed) with all
subsequent scans until you removed it. To remove the reference, select
Manipulate and then Original or load a new method.
Mode
To change the format of the displayed spectrum, choose an option below.
20
Spectrophotometer User Guide
Menu option
Function
ABS
Absorbance.
%T
% Transmittance.
1D
First derivative (records the first derivative of the
Absorbance spectrum).
2D
Second derivative (records the second derivative of the
Absorbance spectrum).
3D
Third derivative (records the third derivative of the
Absorbance spectrum).
Menu option
Function
4D
Fourth derivative (records the fourth derivative of the
Absorbance spectrum).
Peaks menu
This option enables the spectrum to be automatically searched for peaks,
valleys or zero crossing points. To perform a search, select an option in the
menu below and press Enter.
FUNCTION
PEAKS
VALLEYS
PKS & VALLEYS
ZERO CROSS
RATIO
CORR RATIO
PK. HEIGHT
When the search is complete, the spectrum is displayed with the peak
positions marked. For a peak to be found, there must be more than 15 data
points between that point and a previous peak.
The menu options are explained below. For Ratio and Corr Ratio, enter
the wavelengths as prompted. All results can be viewed by pressing View
Results.
Menu Option
Function
Peaks
Marks the 10 highest peaks.
Valleys
Marks the 10 lowest valleys.
Pks & Valleys
Marks the 5 highest peaks and the 5 lowest valleys.
Zero Cross
Marks the first 10 zero crossings.
Ratio
Calculates the ratio λ1/ λ2
Corr Ratio
Calculates the ratio (λ-λ3) / (λ2-λ3)
Pk Height
Calculates the peak maximum relative to a local
baseline.
Spectrophotometer User Guide
21
Smoothing
This option displays a pop-up menu that can be used to apply a SavitzkyGolay smoothing algorithm to the spectrum. You can smooth with a low,
medium or high number of data point. In each case, data points are lost
from both ends of the spectrum.
Smoothing
No. of Points Used
Points Lost at Each End
None
0
0
Low
9
4
Medium
17
8
High
33
16
Original
Use this option to remove any manipulation and display the spectrum as
originally collected and specified by the scan method. It also clears any
reference spectrum that was added with the Compare option. .
Track Table screen
The screen lists the y-axis values of the spectrum for the wavelengths
marked using Track. To access this screen, press the View Table function
key from the Track screen. The format of the measured values (ABS, %T,
Intensity, or 1st, 2nd, 3rd, 4th derivative) depends on the current setting for
the Mode parameter in the Manipulate menu.
The track markers are saved with the spectrum and will be displayed when
the spectrum is reloaded.
Function key
Description
View Graph
Displays the Track Graph which can be used to add or delete
markers.
LIMS Export
Sends the results via the RS-232 port.
Print List
Prints the information in the table using the selected printer.
Scan Graph
Displays the Scan Graph screen.
Track table function keys
22
Spectrophotometer User Guide
Peak Table screen
The screen lists the positions and values of the peaks found by the previous
peak search. To display this screen, press the View Results function key
from the Peaks, Valleys, Pks & Valleys, or Zero Cross option in the Peaks
menu. The format of the found peaks (ABS, %T, Intensity, or 1st, 2nd, 3rd,
4th derivative) depends on the current setting for the Mode parameter in
the Manipulate menu. The list is sorted by wavelength.
Each marker is identified as a peak, valley or zero crossing.
Function key
Description
LIMS Export
Sends the results via the RS-232 port.
Print List
Prints the information in the table using the selected printer.
Scan Graph
Displays the Scan Graph screen.
Peak Table function keys
Ratio Table screen
The screen shows the positions and values of the wavelengths and the ratio
as selected by the Ratio or Corr. Ratio functions. To display this screen,
press the View Table function key from the Ratio or Corr Ratio option in
the Peaks menu.
Function key
Description
View Graph
Displays the Scan Graph screen.
LIMS Export
Sends the results via the RS-232 port.
Print List
Prints the information in the table using the selected printer.
Scan Graph
Displays the Scan Graph screen.
Ratio Table function keys
Spectrophotometer User Guide
23
Peak Height screen
This screen shows the locations and measured values of the peaks selected
with the Pk Height function. To display this screen, press the View Table
function key from the Pk Height option in the Peaks menu.
Function key
Description
View Graph
Displays the Scan Graph screen.
LIMS Export
Sends the results via the RS-232 port.
Print List
Prints the information in the table using the selected printer.
Scan Graph
Displays the Scan Graph screen.
Peak Height function keys
24
Spectrophotometer User Guide
Fixed
Select the Fixed application on the Home screen to measure absorbance
values at up to 20 fixed wavelengths.
Use the Fixed Method screen to set data collection and analysis parameters.
When you are finished setting parameters, press Zero/Base to perform a
baseline scan with the current method. When you are ready to analyze the
first sample, place the sample cell in the sample holder and press Run.
The spectrophotometer performs the measurements and displays the results
on the Fixed Results screen. After all the results have been collected, save
the data.
Fixed method parameters
Use this screen to set instrument and analysis parameters for measuring and
reporting absorbance values at up to 20 fixed wavelengths. To change a
parameter setting, highlight the parameter and press Enter. See Parameter
Entry for more information.
* FIXED *
MODE
TEST NAME
λ SELECT
WAVELENGTH(S)
BANDWIDTH
INTEGRATION
DELAY TIME
LAMP CHANGE
USER
UVcalc
PRINT
METHOD
ABS
SINGLE λ
550.0 nm
2.0 nm
1 s
00:00
325 nm
0
SAVE
METHOD
VIEW
RESULTS
For Zeta, Omega, Evolution 160, UV-10, BioMate 6 models
Spectrophotometer User Guide
25
* FIXED *
MODE
TEST NAME
λ SELECT
WAVELENGTH(S)
BANDWIDTH
INTEGRATION
TIMER(S)
LAMP CHANGE
USER
UVcalc
PRINT
METHOD
ABS
SINGLE λ
550.0 nm
2.0 nm
1 s
0
325 nm
0
SAVE
METHOD
VIEW
RESULTS
For AquaMate models
Parameter
Function
Mode
Selects a format for displaying the data.
ABS – Absorbance
%T - % Transmittance.
Test Name
Displays the Text Entry screen used to enter a descriptive name for the scan method. The Test Name is
saved with the method and any results produced by the method.
λ Select
Selects the number and sequence of wavelengths measured for each sample.
Single λ - Measures each sample at a single wavelength which is the same for each sample.
Multi λ - Allows each sample to be measured at up to 20 wavelengths, which are the same for each
sample.
Serial λ - Allows a single wavelength measurement to be made at a different wavelength for each
sample for up to 9 samples.
Wavelength(s)
Specifies the wavelength values.
Single λ - Enter the required wavelength at the prompt and press Enter.
Multi λ - Select the first wavelength and press Enter to display a pop-up entry box. Enter the wavelength
and press Enter. The instrument displays the Multi λ screen with the next wavelength in the list
highlighted. Up to 20 wavelengths may be entered. When the list is finished press Accept to accept the
new list or Cancel to return to the Fixed Method screen without changing the wavelength list.
Serial λ - Press Enter to display the entry box for the wavelength to be used for the first sample. Data
entry is as for Multi λ above. When the required wavelengths have been entered press Accept to accept
the new list, or press Cancel to return to the Fixed Method screen leaving the original list unchanged.
Bandwidth
This parameter is fixed at 2.0 nm.
Integration
Defines the integration time for which the result is measured. Use the pop-up box to enter a value in
seconds.
Note: The current data will be lost if the integration time is changed.
26
Spectrophotometer User Guide
Parameter
Function
Delay Time
Specifies a delay between pressing Run and the start of the measurement. Enter a value from 0 to 99
minutes and 59 seconds. Use a decimal point to separate minutes and seconds (e.g., 99:59). The number
of seconds must always be entered explicitly.
(not available for
AquaMate models)
Timer(s)
(for AquaMate
models only)
Allows you to add up to 4 timers in a method for specific purposes. For each timer, define the following:
Title – Select a name that indicates the purpose of the timer (Timer, Wait, Shake, Invert, Swirl, Boil or
Heat).
Duration – Specify a delay time from 1 to 100 seconds in digital format with a period separator (e.g., 00.01
to 99.59).
Action – Select whether to display a user prompt when the delay time has passed.
Choose Pause to display a user prompt with three choices (Stop, Zero or Continue).
Choose Continue to skip the user prompt. After the delay time has passed, the system automatically
proceeds to the next task in the measurement sequence.
Note: Timers can be used with a sipper accessory that is in Auto mode. You cannot use a timer with a
cell programmer in Auto mode.
See Timer Function Keys for more information.
Lamp Change
Selects the wavelength at which the source is changed between the tungsten and deuterium lamps.
Select from 315, 320, 325, 330, 335, 340, D2, W.
Note: Selecting D2 or W manually overrides the Lamp Change setting and the selected lamp will be used
regardless of the wavelength set.
Note: This parameter is not available for the AquaMate Vis.
User
Displays the Text Entry screen to enter a user name. The user name is automatically saved with the
method and any data produced by the method.
Note: If User Log-on is in operation, the user name cannot be changed.
UVcalc
Displays the UVcalc screen.
Function key
Description
View Results
Displays the Fixed Results screen.
Save Method
Displays the Save screen, which allows the method to be saved to a USB memory device.
Print Method
Prints the current method parameters using the selected printer.
Note
If the selected wavelength requires the deuterium lamp, the lamp will
activate automatically. The current data will be lost if the wavelength is
changed.
Pressing Run starts a fixed measurement using the current method and then
switches to the Fixed Results screen.
Spectrophotometer User Guide
27
Pressing Zero starts a zero using the current method.
Note
Any changes to the Wavelength, Bandwidth, Integration or Lamp Change
parameters will invalidate the current results.
If Autoprint is selected (see Setup for details), a change to the Mode
parameter will invalidate the current results.
Timer function keys
Function key
Description
Change Mode
Sets the operating mode for the timers.
Single Use – Runs all timers before the first measurement only.
Multiple Use – Runs all timers before each measurement.
Run Timers
Runs the timers without initiating a measurement sequence.
Accept
Stores the timer settings and displays the Fixed Method screen.
Cancel
Cancels the timer settings and displays the Fixed Method screen.
If one or more timers are defined in a method, the first timer starts when
you press Run. The system shows the remaining time for the current timer.
If you need to stop the timer, press Stop. If you allow the timer to continue
and no user prompt is defined, after the delay time has passed, the system
automatically proceeds to the next task in the measurement sequence.
If the timer includes a user prompt, the prompt appears with the following
options:
Stop – Interrupts the measurement and displays the Fixed Results screen.
Zero – Takes a baseline measurement.
Proceed – Continues to the next task in the measurement sequence.
After the last timer is completed, the system proceeds to the next
measurement task.
28
Spectrophotometer User Guide
Fixed Results screen
The layout of the screen depends on the current settings for the Mode and
λ Select parameters on the Fixed Method screen.
Parameter
Function
Single λ
In ABS or %T modes, up to 2 columns of results are displayed
per page.
Multi λ
Two columns of results are displayed per page. Results of each
sample always start on a new page.
Serial λ
One column of results is displayed per page. Results accumulate
on the same page until it is full.
Function key
Description
Clear Results
All results are cleared, ready to start the next batch.
Fixed Page
Displays the Fixed Method screen
Save Data
Displays the Save screen, which allows the results to be saved
to a USB memory device.
Print List
Prints the current list using the selected printer.
LIMS Export
Sends the results via the RS-232 port.
Use the up/down arrow keys to display the previous or next page of results.
Results are numbered sequentially, up to 600 samples per batch.
Press Run to take another sample measurement.
Press Zero/Base to zero the instrument at the wavelength(s) specified in the
method.
Spectrophotometer User Guide
29
Quant
Select the Quant application on the Home screen to measure absorbance
values and compare them to a concentration curve in order to determine
sample concentration.
Use the Quant Method screen to set data collection and analysis
parameters. When you are finished setting parameters, press Zero/Base to
perform a baseline scan with the current method. When you are ready to
analyze the first sample, place the sample cell in the sample holder and
press Run.
The spectrophotometer performs the measurement and displays the result
on the Quant Results screen. After all the results have been collected, save
the data.
Quant method parameters
Use this screen to set instrument and analysis parameters for performing
quantitative measurements. To change a parameter setting, highlight the
parameter and press Enter. See Parameter Entry for more information.
* QUANT *
TEST NAME
WAVELENGTH
BANDWIDTH
INTEGRATION
STANDARDS
REPLICATES
UNITS
CURVE FIT
LAMP CHANGE
USER
UVcalc
MEASURE STDS
CALIBRATE
PRINT
METHOD
550.0 nm
2.0 nm
2 s
0
3
LINEAR
325 nm
0
YES
SAVE
METHOD
VIEW
RESULTS
VIEW
CALIB
For Zeta, Omega, Evolution 160, UV-10, and BioMate 6 models
Spectrophotometer User Guide
30
* QUANT *
TEST NAME
WAVELENGTH
BANDWIDTH
INTEGRATION
STANDARDS
REPLICATES
UNITS
CURVE FIT
MEASURE STDS
TIMER(S)
LAMP CHANGE
USER
UVcalc
CALIBRATE
PRINT
METHOD
550.0 nm
2.0 nm
2 s
0
3
LINEAR
YES
0
325 nm
0
SAVE
METHOD
VIEW
RESULTS
VIEW
CALIB
For AquaMate models
Option
Function
Test Name
Displays the Text Entry screen used to enter a descriptive name for
the quant method. The Test Name is saved with the method and any
results produced by the method.
Wavelength
Specify a wavelength for measuring the samples. Enter a value
between 190.0 nm and 1100.0 nm (or between 325 and 1100nm for
the AquaMate Vis).
If the selected wavelength requires the deuterium lamp, the lamp
will activate automatically.
Note: The current data will be lost if the wavelength is changed.
Bandwidth
This parameter is fixed at 2.0 nm.
Integration
Defines the integration time for which the result is measured. Enter
a value between 1 and 9999 seconds.
Standards
Displays the Standards screen for entering concentrations values
for the method standards. See “Standards screen” in the next
section for details.
Replicates
Defines the number of times each standard will be measured (1–3).
All values are used in the calibration.
Units
Displays the Text Entry screen used to enter the concentration unit
for the standards.
Spectrophotometer User Guide
31
Option
Function
Curve Fit
Selects the curve fit algorithm used in the calibration.
Linear - Performs a linear calibration. At least two standards are
required.
Linear to 0 - Performs a linear calibration forced through zero.
Quadratic - Performs a quadratic fit on the data. At least three
standards are required.
Quad to 0 - Performs a quadratic fit with the data forced through
zero. At least two standards are required.
Measure Stds
Toggles between internal calibration (Yes) and external calibration
(No).
Choose Yes to measure the standards and calibrate using the
concentrations entered on the Standards screen. When ready,
press Calibrate. At the prompt, insert the first standard in the beam
and press Run. Repeat for the remaining standards in order.
Choose No to specify absorbance values for the standards from an
external calibration. When ready, press Calibrate. Enter the
absorbance value of the first standard at the prompt and press
Enter. Repeat for the remaining standards in order.
Timer(s)
(for
AquaMate
models only)
Allows you to add up to 4 timers in a method for specific purposes.
For each timer, define the following:
Title – Select a name that indicates the purpose of the timer (Timer,
Wait, Shake, Invert, Swirl, Boil or Heat).
Duration – Specify a delay time from 1 to 100 seconds in digital
format with a period separator (e.g., 00.01 to 99.59).
Action – Select whether to display a user prompt when the delay
time has passed.
Choose Pause to display a user prompt with three choices (Stop,
Zero or Continue).
Choose Continue to skip the user prompt. After the delay time has
passed, the system automatically proceeds to the next task in the
measurement sequence.
Note: Timers can be used with a sipper accessory that is in Auto
mode. You cannot use a timer with a cell programmer in Auto mode.
See Timer Function Keys for more information.
32
Spectrophotometer User Guide
Option
Function
Lamp Change
Selects the wavelength at which the source is changed between
the tungsten and deuterium lamps. Select from 315, 320, 325, 330,
335, 340, D2, W.
Note: Selecting D2 or W manually overrides the Lamp Change
setting and the selected lamp will be used regardless of the
wavelength set.
Note: This parameter is not available for the AquaMate Vis.
User
Displays the Text Entry screen used to enter a user name. The user
name is automatically saved with the method and any data
produced by the method.
Note: Changing the user name will not cause any current data to be
lost.
Note: If User Log-on is in operation, the user name cannot be
changed.
UVcalc
Displays the UVcalc screen. See UVcalc for more information.
Function key
Description
View Calib
Displays the Quant Graph screen if valid calibration exists.
View Results
Displays the Quant Results screen if sample results exist for this
method.
Save Method
Displays the Save screen, which allows the method to be saved to a
USB memory device.
Print Method
Prints the current method parameters and the standards table using
the selected printer.
Calibrate
Displays the Standards screen.
Note
The current data will be lost if the integration time is changed.
Note
Changing the standards will cause any current data to be lost.
Note
Changing the curve fit will cause the existing calibration to be recalculated.
Any results associated with the previous calibration will be lost.
Note
Any current data will be lost if the lamp changeover wavelength is
changed.
Spectrophotometer User Guide
33
Timer function keys
Function key
Description
Change Mode
Sets the operating mode for the timers.
Single Use – Runs all timers before the first measurement only.
Multiple Use – Runs all timers before each measurement.
Run Timers
Runs the timers without initiating a measurement sequence (for
example, when collecting standards for a method that uses timers).
Accept
Stores the timer settings and displays the Fixed Method screen.
Cancel
Cancels the timer settings and displays the Fixed Method screen.
If one or more timers are defined in a method, the first timer starts when
you press Run. The system shows the remaining time for the current timer.
If you need to stop the timer, press Stop. If you allow the timer to continue
and no user prompt is defined, after the delay time has passed, the system
automatically proceeds to the next task in the measurement sequence.
If the timer includes a user prompt, the prompt is displayed with the
following options:
Stop – Interrupts the measurement and displays the Fixed Results screen.
Zero – Takes a baseline measurement.
Proceed – Continues to the next task in the measurement sequence.
After the last timer is completed, the system proceeds to the next
measurement task.
Quant Standards screen
This screen lists the standards for the Quant method. Before the system can
be calibrated, each standard must have a concentration entered.
To enter the concentration values of the standards, select a standard and
press Enter to display the concentration entry box. Enter the concentration
of the standard and press Enter. The Standards screen is displayed with the
new value displayed and the next standard highlighted. Up to 20 standards
can be specified.
34
Spectrophotometer User Guide
When all the standards have been entered, press Accept to return to the
Quant Method screen with the new list of standards, or Cancel to return
leaving the old list unchanged.
If a calibration has been done, the correlation coefficient and the equation
are displayed.
If a calibration has not been done, pressing Run causes the warning prompt
"CANNOT RUN WITHOUT CALIBRATION" to appear. Otherwise it
takes a sample measurement and switches to the Quant Results screen.
Pressing Zero/Base starts a zero using the current method.
Function key
Description
View Calib
Displays the Quant Graph screen if valid calibration exists.
View Results
Displays the Quant Results screen if sample results exist for
this method.
Quant Page
Displays the Quant screen.
Edit Std (appears
after calibration)
Allows you to specify whether each standard will be used,
ignored or re-measured.
Edit Curve
(appears after
calibration)
Allows you to change the curve fit.
Quant Standards function keys
Quant Calibration screen
Press Zero/Base to zero the instrument with the current method.
To start the calibration, display the Quant Method screen and press
Calibrate. The Quant Calibration graph is displayed and the instrument
prompts for each standard (and replicate) in turn. As the measurements of
the standards proceed, the data points are marked on the graph. When all
the standards have been measured, the system calculates the equation,
rescales the graph and then draws and displays the line of best fit on the
graph.
To stop the calibration, press Stop. The calibration is aborted and the
software returns to the Quant Standards screen. Any values obtained are
lost.
Spectrophotometer User Guide
35
Press Run to start the first sample measurement. The sample results appear
automatically on the Quant Results screen.
Note
If you press Run before the calibration step is completed, the message
"CANNOT RUN WITHOUT CALIBRATION" is displayed. Press ESC
to clear the error message.
Function key
Description
View Results
Displays the Quant Results screen if sample results exist for
this method.
Quant Page
Displays the Quant screen.
Standards
Displays the Standards screen.
Print Graph
Prints the Quant method and calibration graph.
Save Method
Calibrate function keys
Quant Results screen
Results are numbered sequentially, up to 600 samples per batch. Use the
up/down arrow keys to display the previous or next page of results.
Press Run to take another sample measurement. The results are displayed
automatically.
Function key
Description
Clear Results
Deletes all data from the Quant Results table.
Quant Page
Displays the Quant screen.
Save Data
Displays the Save screen.
Print List
Prints the Quant Results using the selected printer.
LIMS Export
Sends the results via the RS-232 port.
Quant Results function keys
36
Spectrophotometer User Guide
Rate
Select the Rate application on the Home screen to measure absorbance at
one wavelength over a period of time.
Use the Rate Method screen to set data collection and analysis parameters.
When you are ready to analyze the first sample, press Zero to zero the
instrument. Then place the sample cell in the sample holder and press Run.
The spectrophotometer performs the measurements and displays the result
on the Rate Graph screen. From there the spectrum can be manipulated
and saved to a Library or USB memory device.
Spectrophotometer User Guide
37
Rate Method screen
Use this screen to set instrument and analysis parameters for measuring and
reporting absorbance values collected over a period of time. To change a
parameter setting, select the parameter and press Enter. See Parameter
Entry for more information.
Note
The current data will be lost if any of the method parameters (except for
the Test Name, Slope, Factor, Units and User name) are changed.
* RATE *
TEST NAME
WAVELENGTH
BANDWIDTH
MEASURE TIME
DELAY TIME
ABS DISPLAY
GRAPH HIGH
GRAPH LOW
FACTOR
UNITS
LAMP CHANGE
USER
CHART HIGH
CHART LOW
PRINT
METHOD
340.0 nm
2.0 nm
00:30
00:00
ABSOLUTE
2.000
0.000
1.000
325 nm
:
6.0000
3.0000
SAVE
METHOD
VIEW
GRAPH
VIEW
RESULTS
For Zeta, Omega, Evolution 160, UV-10, AquaMate Plus, AquaMate Vis
models
38
Spectrophotometer User Guide
* RATE *
TEST NAME
RATE MODE
WAVELENGTH
BANDWIDTH
MEASURE TIME
DELAY TIME
ABS DISPLAY
GRAPH HIGH
GRAPH LOW
FACTOR
UNITS
LAMP CHANGE
USER
CHART HIGH
CHART LOW
PRINT
METHOD
SERIAL
340.0 nm
2.0 nm
00:30
00:00
ABSOLUTE
2.000
0.000
1.000
325 nm
6.0000
-3.0000
SAVE
METHOD
VIEW
GRAPH
VIEW
RESULTS
For BioMate 6 model
Menu Item
Function
Test Name
Use the Text Entry screen to enter a descriptive name for the method. The Test Name is saved with the
method and any spectra produced by the method.
Rate Mode
When the 7-Cell Changer is installed, Rate Mode toggles between the Serial and Parallel settings:
Parallel - Rate measurements for up to 7 samples may be made in parallel. In this mode, MEASURE
INTERVAL sets the time between each cycle, i.e. the length of time between successive measurements on
the first sample. The number of measurements taken on each sample is set by the MEASURE CYCLES
parameter. The total time over which the measurements are made is the product of the MEASURE
INTERVAL and the MEASURE CYCLES. For example, an analysis using 4 cells with MEASURE INTERVAL
set to 15 seconds and MEASURE CYCLES set to 20 seconds would give a total measurement time of
5 minutes.
Serial - Each sample is measured individually. In this mode, MEASURE TIME replaces MEASURE
INTERVAL and sets the total time over which the sample is measured. (Use this setting when you want the
7-cell changer to behave like a single cell holder.)
When the single cell holder is installed, Rate Mode is automatically set to Serial. .
Wavelength
Selects a wavelength for measuring the samples. Enter a value between 190 nm and 1100 nm (or between
325 and 1100nm for the AquaMate Vis).
If the selected wavelength requires the deuterium lamp, the lamp will activate automatically.
Note: Any current data will be lost if the wavelength is changed.
Bandwidth
This parameter is fixed at 2.0 nm.
Spectrophotometer User Guide
39
Menu Item
Function
Measure Time
Defines the time period over which the sample will be measured. Enter a value in the range between 00:05
seconds and 99:59 seconds in steps of 1 second.
If the Cell Changer is On, the measurement time sets the time between individual measurements on the
first cell (i.e., the time for each cycle).
Delay Time
Sets the time between pressing Run and the start of the measurement. Enter a value in the range between
00:05 and 99:59 seconds in steps of 1 second.
Abs Display
Toggles the graphical display between the Absolute Values and Relative Values settings.
Absolute Values – Plots the measured absorbance values in Absorbance vs. Time. When Abs Display =
Absolute, the Graph High and Graph Low parameters appear on the Rate Method screen.
Relative Values – Plots the measured absorbance values relative to the first measurement (subtracts the
first measured absorbance value from all subsequent measurements). This causes the plot to start at the
0,0 coordinate (change in Absorbance vs. Time). When Abs Display = Relative, the Slope and Range
parameters appear on the Rate Method screen.
Graph High
Sets the maximum y-axis value on the displayed graph. Enter a value from 0.010 to 3.000.
Note: This parameter appears only when ABS DISPLAY is set to ABSOLUTE.
Graph Low
Sets the minimum y-axis value on the displayed graph. Enter a value in the range between 0.010 and 2.990.
Note: This parameter appears only when ABS DISPLAY is set to ABSOLUTE.
Slope
Sets the graph to display positive or negative changes in Absorbance.
Positive – Select this option if Absorbance increases with time.
Positive – Select this option f Absorbance decreases with time.
Note: This parameter appears only when ABS DISPLAY is set to RELATIVE.
Range
This sets the graph y-axis. Enter a number in the range between 0 and 3 A. Enter a number slightly larger
than the expected change in Absorbance.
Note: This parameter appears only when ABS DISPLAY is set to RELATIVE.
Factor
Enter the factor for Activity as a number in the range between 0.001 and 9999.999.
Units
Displays the Text Entry screen used to enter the units of Activity. Enters the required description or units of
Activity up to 11 alphanumeric characters.
Lamp Change
Selects the wavelength at which the source is changed between the tungsten and deuterium lamps.
Select from 315, 320, 325, 330, 335, 340, D2, W.
Note: Selecting D2 or W manually overrides the Lamp Change setting and the selected lamp will be used
regardless of the wavelength set.
Note: This parameter is not available for the AquaMate Vis.
40
Spectrophotometer User Guide
Menu Item
Function
User
Displays the Text Entry screen to enter a user name. The user name is automatically saved with the
method and any data produced by the method. Changing the user name will not cause the current data to
be lost.
Note: If User Log-on is in operation, the user name cannot be changed.
Chart High
Sets the maximum y-axis value on the analog output. Select from -2.9999 to 6.0000.
Chart Low
Sets the minimum y-axis value on the analog output. Enter a number in the range between -3.0000 and
5.9999.
Number of Samples
Available only when the Cell Changer is installed and ABS Display is set to Parallel. See Parallel Rate
Measurements using the Cell Changer for more information.
Measure Cycles
Available only when the Cell Changer is installed and ABS Display is set to Parallel. See Parallel Rate
Measurements using the Cell Changer for more information.
Function key
Description
View Results
Displays the Rate Results screen.
View Graph
Displays the Rate Graph screen.
Save Method
Displays the Save screen, which allows the method to be saved to a USB memory device.
Print Method
Prints the current method parameters using the selected printer.
Rate Graph screen
This screen displays the Rate curve and allows it to be manipulated.
Function key
Description
View Results
Displays the Rate Results screen.
Rate Page
Displays the Rate Method screen.
Save Data
Displays the Save screen, which allows the method and data to be
saved to a USB memory device.
Print
Prints the rate graph and the results using the selected printer. (If
a Cell Programmer is fitted, see the note below.)
Manipulate
Displays the Manipulate pop-up menu (see descriptions below).
Rate Graph function keys
If you are using the Cell Changer to run more than one rate in parallel,
three more print options are available:
Spectrophotometer User Guide
41
All Overlay
All Sequential
One Result
Prints the results of all cells in the run on one sheet of paper up to a
maximum of 4 results. If more than four results are present, the rest are
printed on a second sheet.
Prints each result in the run on a separate sheet of paper.
Prints only the displayed results.
Press Run to start a measurement using the current method.
Press Zero/Base to zero the instrument using the wavelength specified in the
current method.
Manipulate menu options
MANIPULATE
TRACK
RESCALE
SECTION
SMOOTHING
ABS DISPLAY
ORIGINAL
ANOTHER CELL
42
Spectrophotometer User Guide
Item
Function
Track
Sets the start and stop time for the rate calculation.
Rescale
Changes x- and y-axis scales automatically or
manually.
Section
Sets sequential start and stop times to enable rates to
be calculated on up to four sections of the rate curve.
Smoothing
Allows three levels of smoothing to be applied to the
Rate Curve.
ABS Display
Toggles the graphical display between Absolute Values
(plots the measured absorbance of each sample vs.
time) and Relative Values (plots the change in
absorbance relative to the first measurement over
time.)
Original
Resets the graph to display the data as originally
collected.
Another Cell
Only present if the Cell Changer has been used.
Enables the results of another cell from the same run to
be displayed.
Track
Use the arrow keys to move the cursor (vertical line) across the screen. The
cursor always moves to a data point regardless of the displayed scales.
Pressing Enter places a marker at the current time.
To delete a marker, place the cursor on the marker and press Clear.
The x-axis values are used to recalculate the rate of change of Absorbance
between the new start and stop times. Results are listed on the Rate Results
screen.
Up to four discrete pairs of cursors can be placed on the graph. Arrows are
placed on the cursors and results are displayed on the Rate Results screen
for those parts of the graph indicated by the arrows. The minimum interval
between Track cursors is one second.
Selecting Section will remove the Track markers.
Track graph function keys
Function key
Description
View Results
Displays the Rate Results screen.
Fast/Slow
Toggles between two cursor speeds. In Fast mode, the cursor
jumps 5% of the graph or to the next data point, whichever is
greater. In Slow mode, the cursor jumps to the next data point or
the next display pixel, whichever is greater. The function key
label shows the deselected speed, i.e., the opposite of the one
you are currently using.
Clear All
Deletes all the markers from the Track Graph.
Print
Prints the displayed data using the selected printer.
Rate Graph
Displays the Rate Graph screen.
Track screen function keys
Section
Use the arrow keys to move the cursor (vertical line) across the screen. The
cursor always moves to a data point regardless of the displayed scales.
Pressing Enter places a marker at the current time.
To delete a marker, place the cursor on the marker and press Clear.
Spectrophotometer User Guide
43
Up to five markers can be placed on the graph. Rate results will be reported
between markers providing a maximum of four sets of results (Sections).
The minimum Section size is one second.
Results are listed on the Rate Results screen.
Selecting Track will remove the Section markers.
Rescale
This option displays a pop-up menu for changing the graph y-axis scale.
The options available in the menu depend on the current setting for the
ABS Display option in the Manipulate menu described above.
Absolute Absorbance
If ABS Display (Manipulate menu) is set to Absolute Values, the Rescale
menu has the following options:
RESCALE
AUTO
GRAPH HIGH
GRAPH LOW
Relative Absorbance
Option
Function
Auto
Displays the Rate Graph with the y-axis rescaled so that
the trace fills the screen.
Graph High,
Graph Low
Sets the upper and lower y-axis limits for the Rate
Graph.
If ABS Display (Manipulate menu) is set to Relative Values, the Rescale
menu has the following options:
RESCALE
AUTO
RANGE
44
Spectrophotometer User Guide
Option
Function
Auto
Displays the Rate Graph with the y-axis rescaled so that
the trace fills the screen.
Range
Allows the user to set the upper y-axis limit.
ABS Display
This option toggles the graphical display between the Absolute Values and
Relative Values settings.
Absolute Values – Plots the measured absorbance values in Absorbance vs.
Time.
Relative Values – Plots the measured absorbance values relative to the first
measurement (subtracts the first measured absorbance value from all
subsequent measurements). This causes the plot to start at the 0,0
coordinate (change in Absorbance vs. Time).
Smoothing
This option displays a pop-up menu that can be used to apply a SavitzkyGolay smoothing algorithm to the data. You can smooth with a low,
medium or high number of data point. A moving point average is
performed on the data and, in each case, data points are lost from both
ends of the data.
Smoothing
No. of Points Used
Points Lost at Each End
None
0
0
Low
9
4
Medium
17
8
High
37
18
Original
Use this option to remove any manipulation and display the rate graph as
originally specified by the rate method.
Another Cell
If you used the Cell Changer to run more than one rate in parallel, use this
function to select and display the results from any cell in the run. Enter the
number of the cell you wish to display.
Spectrophotometer User Guide
45
Rate Results screen
This screen displays the Initial and Final Absorbance, Initial and Final
Time, the change in absorbance per minute, calculated Activity,
Correlation Coefficient of the best fit line and the smoothing parameter
used.
If the rate curve has been tracked, the Initial and Final Absorbance with the
Initial and Final Time will reflect those chosen by the two cursors.
Function key
Description
View Graph
Displays the Rate Graph.
Rate Page
Displays the Rate Method screen
Save Data
Displays the Save screen for saving methods and data to a USB
memory device.
Print
Prints the Rate Graph and Rate Results using the selected
printer.
LIMS Export
Sends the results via the RS-232 port.
Rate Results function keys
Parallel Rate measurements using the Cell Changer
The 7-Cell Changer can be used in conjunction with the Rate software to
measure between 2 and 7 cells in parallel.
For the Cell Changer to be used it must be On with Mode set to Auto.
The Rate Method setup and Manipulate functions are exactly the same
except that the Measure Time now sets the time between each cycle;
i.e., the length of time between measurements on the first cell. The number
of measurements taken on each cell is set by the Cell Cycles parameter on
the Cell Changer screen. The total time over which the measurements are
made is the result of the time between measurements (Measure Time) and
the number of measurements (Cell Cycles). For example, an analysis using
four cells with Measure Time set to 15 seconds and Cell Cycles set to 20
would give a total measurement time of 5 minutes.
46
Spectrophotometer User Guide
To set up a parallel rate method, following these steps:
1. Set up the method as normal on the Rate Method screen.
Note
Measure Time specifies the time between each measurement on the first
cell.
2. Display the Cell Changer screen.
Press Home then Accessories and select Cell Prog.
3. Set up the Cell Changer method as required.
4. Return to the Rate Method screen and press Zero/Base to zero the
instrument (if required).
5. Press Run to start the analysis
The rate graph shows the data for the first cell.
To view the result of any other cell, press Manipulate on the Rate Graph
screen and select Another Cell. The cell number currently displayed is
shown to the right of the ID: line.
To print the results, press Print from the Rate Graph screen or the Rate
Results screen. The following options appear:
All Overlay – Prints the results of all cells in the run together in batches of
4. Track and Section markers are not included.
All Sequential – Prints each result in the run separately. Track and Section
markers are included.
One Result – Prints the result currently displayed. Track and Section
markers are included.
Spectrophotometer User Guide
47
Multicomponent Analysis (MCA)
Select the MCA application on the Home screen to measure up to 20
components in a sample mixture. You can set up the method to measure
up to 20 wavelengths per sample.
Standards can be measured at run time or loaded from files obtained using
the Multi λ function available from the Fixed application.
Use the MCA Method screen to set data collection and analysis parameters.
When you are finished setting parameters, press Zero/Base to perform a
baseline scan with the current method. When you are ready to start the
analysis, place the sample cell in the sample holder and press Run.
The spectrophotometer performs the measurement and displays the result
on the MCA Results screen. After all the results have been collected, save
the data.
MCA Method screen
Use this screen to set instrument and analysis parameters for performing a
multi-component analysis. To change a parameter setting, highlight the
parameter and press Enter. See Parameter Entry for more information.
* MCA *
TEST NAME
MEASURE STDS
STANDARDS
UNITS
WAVELENGTH(S)
BANDWIDTH
INTEGRATION
DELAY TIME
LAMP CHANGE
USER
CALIBRATE
PRINT
METHOD
YES
0
1
2.0 nm
1 s
00:00
325 nm
SAVE
METHOD
VIEW
RESULTS
VIEW
CALIB
Spectrophotometer User Guide
48
Item
Function
Test Name
Displays the Text Entry screen used to enter a descriptive
name for the quant method. The Test Name is saved with the
method and any results produced by the method.
Measure Stds
Toggles between internal calibration (Yes) and external
calibration (No).
Choose Yes to measure the standards with the run. All fields
remain editable.
Choose No to load standards from a Library or USB memory
device. The Wavelength(s), Bandwidth, Integration, Delay time,
Lamp change and User Name are also loaded with the
standards and cannot be edited. Any attempt to do so causes
the prompt “Change method by loading MCA standards” to
appear and no action is taken.
Standards
Displays the Standards screen for entering concentrations
values for up to 20 method standards. See MCA Standards
screen for details.
Units
Displays the Text Entry screen used to enter the concentration
unit for the standards.
Wavelength(s)
Specify up to 20 wavelengths for measuring the samples. Each
wavelength can be between 190 nm and 1100 nm (or 325 and
1100nm for the AquaMate Vis).
If the selected wavelength requires the deuterium lamp, the
lamp will activate automatically.
Note: The current data will be lost if the wavelength is
changed.
Bandwidth
This parameter is fixed at 2.0 nm.
Integration
Defines the integration time for which the result is measured.
Enter a value between 1 and 9999 seconds.
Delay Time
Specifies a delay between pressing Run and the start of the
measurement. Enter a value from 0 to 99 minutes and 59
seconds. Use a decimal point to separate minutes and seconds
(e.g., 99.59). The number of seconds must always be entered
explicitly.
Note: This field is only available if UVcalc is installed.
Spectrophotometer User Guide
49
Item
Function
Lamp Change
Selects the wavelength at which the source is changed
between the tungsten and deuterium lamps. Select from 315,
320, 325, 330, 335, 340, D2, W.
Note: Selecting D2 or W manually overrides the Lamp Change
setting and the selected lamp will be used regardless of the
wavelength set.
Note: This parameter is not available for the AquaMate Vis.
User
Displays the Text Entry screen to enter a user name. The user
name is automatically saved with the method and any data
produced by the method. Changing the user name will not
cause any current data to be lost.
Note: If User Log-on is in operation, the user name cannot be
changed.
50
Spectrophotometer User Guide
Function key
Description
View Results
Displays the Results table if it contains results.
View Stds
Displays the Standards results table if a calibration has been
performed.
Save Method
Displays the Save screen, which allows the method to be saved
to a USB memory device.
Print Method
Prints the current method parameters using the selected
printer.
Calibrate
Displays the Calibration screen to perform a calibration if
standards and wavelengths have been entered.
Note
Changing the Measure Stds parameter will cause all previous data to be
lost.
Note
The current data will be lost if the integration time is changed.
Note
The current data will be lost if the lamp change parameters are changed.
MCA Standards screen
When Measure Stds is set to Yes, this screen lists the standards for the
MCA method. Before the system can be calibrated, each standard must
have a name and associated concentration entered.
To define the standards, select a standard and press Enter. The Text Entry
screen is displayed. Enter the name of the standard and press Accept. The
instrument returns to the Standards screen and the concentration field is
highlighted for the current standard. Press Enter to display the pop-up
entry box. Use the numeric keys to enter the concentration of the standard
and press Enter. The Standards screen is displayed with the next standard
highlighted. Up to 20 standards can be specified with up to 10 standards
per page. (Press Std11-Std20 to display page 2 or Std1-Std10 to display
page 1).
When Measure Stds is set to No, press Enter to display the Library screen.
Use the Library functions to select and load the file for each standard in
sequence.
Note
Use the Multi λ function available from the Fixed application to build a
library of standards for your multi-component analysis. If you do that,
make sure you use the same method every time you run your standards and
those conditions will be used in your MCA automatically.
The same method must be used for each Multi λ result, and will be used
for the MCA analysis. When the first Multi λ file is loaded, the current
MCA method is changed to that used to obtain the Multi λ result.
Standards can thus be used in any combination without having to
recalibrate for each new mixture.
Function key
Description
Std1-Std10
Std11-Std20
Toggles between page 1 and page 2 of the Standards screen.
Accept
Accepts any changes made to the Standards list and displays
the MCA Methods screen.
Cancel
Deletes any changes made to the Standards list and displays
the MCA Methods screen.
MCA Standards function keys
Spectrophotometer User Guide
51
MCA Wavelength screen
This screen lists the wavelengths to be measured. You can measure up to
20 wavelengths. You must enter at least one wavelength for each standard.
To enter the wavelengths, select a wavelength and press Enter. The
wavelength entry box is displayed. Enter the wavelength and press Enter.
You do not need to enter them in numerical order; however, the analysis
will be faster if you do. The Wavelength screen is displayed with the new
value displayed and the next wavelength highlighted. When all wavelengths
have been entered, press Accept to return to the MCA method screen with
the new list of wavelengths or Cancel to return leaving the old list
unchanged.
Alternatively wavelengths may be entered directly from a scan. Clear the
beam(s) and press Zero Base to perform a baseline scan, then put the sample
in the cell holder and press Scan Graph. The instrument performs a scan
using the method currently entered in the Scan Parameters screen.
Move the vertical cursor to a suitable wavelength and press Enter to mark
it. Repeat until all required wavelengths have been marked. Marks can be
removed using Clear, or Clear All. When all required wavelengths have
been marked, press Accept to accept the list and return to the MCA
Methods screen.
Wavelengths can be selected either from a scan of the mixture to be
analyzed or by performing a scan on each standard in turn and selecting
suitable wavelengths. Wavelengths already entered in the table are shown
on the Scan Graph.
Function key
Description
Accept
Accepts any changes made to the Wavelength list and displays
the MCA Methods screen.
Fast/Slow
Toggles between two cursor speeds. In Fast mode, the cursor
jumps 5% of the graph or to the next data point, whichever is
greater. In Slow mode, the cursor jumps to the next data point
or the next display pixel, whichever is greater.
Clear All
Clears all marks.
Rescale
Rescales the x- and y-axes so that the spectrum fills the
screen.
MCA Wavelengths function keys
52
Spectrophotometer User Guide
MCA Calibration screen
Before you calibrate, use the MCA Standards screen to define the standards
and the MCA Wavelengths screen to specify the wavelengths to be
measured.
When you are ready, clear the beam(s) and press Zero Base to perform a
baseline scan.
To start the calibration, display the MCA Method screen and press
Calibrate.
Notice
If the results from a previous calibration are displayed, you will be given the
option to proceed or cancel. If you select Proceed, the previous data will be
lost.
Press Enter to start the calibration. The message “Press Run to measure
STD1:” is displayed. Place the first standard in the beam and press Run.
Continue until all standards have been measured. The results for each
standard are stored in the Calibration Results Table; the data for each
standard is displayed on a new page. Use the arrow keys to display the next
or previous page.
When you have finished measuring the standards, place the first sample in
the beam and press Run (see the next section for more information).
Analyzing a sample
When a calibration has been performed, or loaded with the method, the
MCA application is ready to use.
To measure a sample, place the sample in the beam and press Run from the
MCA Method or Results screen. The instrument measures the absorbance
of the sample at each of the wavelengths specified in the method and
compares it with the absorbance values of the standards at these
wavelengths. The concentration of each component is calculated and
displayed on the Results screen. The results for each sample are displayed
on a new page. Use the arrow keys to display the next or previous page.
Spectrophotometer User Guide
53
54
Spectrophotometer User Guide
Function key
Description
Clear Results
Clears the Results Table.
MCA Page
Displays the MCA Method screen.
Save Data
Displays the Save screen for saving data to the Library or a
USB memory device.
Print List
Prints the data using the selected printer.
Bio Tests
This section applies to the BioMate 6 spectrophotometers only.
The BioMate 6 provides an assortment of general tests used to characterize
biological and biochemical substances. These tests fall into the following
categories:
•
•
•
Nucleic acid measurements
Protein measurements
Cell growth analysis
These tests are accessed from the BioMate Tests screen, which appears
when you start the instrument or press Home.
* BIOMATE TESTS *
NUCLEIC ACID TESTS
PROTEIN TESTS
CELL GROWTH
OLIGO CALCULATOR
LIBRARY
SETUP
Note
GENERAL
TESTS
STORED
TESTS
REMOTE
To display the default Home screen for Local Control Software, from the
BioMate Tests start-up screen choose General Tests.
BioMate applications
All of the parameters for the BioMate applications described in this section
are factory set. This means if you want to change the parameters, you will
need to save them with a different name.
Note
See BioMate 6 Test Parameters for a list of all the parameters used in each
pre-set test. See Calculations for BioMate 6 Tests for a list of calculations
used by each test.
Spectrophotometer User Guide
55
Nucleic acid measurements
These tests can be used to determine the concentration and purity of
nucleic acid in an unknown sample.
DNA – Measures absorbance at 260 nm and 280 nm or at 260 nm and
230 nm and determines concentration and purity based on the absorbance
ratio and absorbance difference.
DNA with scan – Records absorbance scan between 260 nm and 280 nm
or between 260 nm and 230 nm and determines concentration and purity
based on the absorbance ratio and absorbance difference.
DNA/RNA Conc – Measures absorbance at 260 nm and calculates
concentration based on the absorbance and concentration factor.
RNA – Measures absorbance at 260 nm and calculates concentration based
on the absorbance and concentration factor.
Oligonucleotides – Measures absorbance at 260 nm and calculates
concentration based on either the absorbance and concentration factor or
the absorbance and concentration factor determined by the oligo calculator
application.
Several of these categories include multiple tests that are similar. For
example, the parameters are the same for the Direct UV measurement of
ssDNA and RNA tests, but the factor used to convert absorbance to
concentration is different. Similarly, the parameters for the Direct UV
measurement of oligonucleotide tests are also the same but the factors used
to convert absorbance to concentration are different. Screen images are
provided as examples but are not comprehensive. For a complete list of all
parameters and calculations for each test, refer to the appendices.
To change a parameter setting, highlight the parameter and press Enter. See
Parameter Entry for more information.
DNA tests
This group includes two tests that function almost identically—the only
difference is in the wavelengths used for the measurements (one measures
absorbance at 260 nm and 280 nm; the other at 260 and 230 nm.)
56
Spectrophotometer User Guide
The example below shows the parameters for the DNA (260/280) test. For
the DNA (260/230) test, Wavelength 2 is set to 230 nm.
* DNA (260/280) *
TEST NAME
WAVELENGTH 1
WAVELENGTH 2
REF. WAVELENGTH CORRECTION
DNA FACTOR (λ1)
DNA FACTOR (λ2)
DISPLAY PROTEIN
DILUTION MULTIPLIER
UNITS
ID# (0=OFF)
AUTOPRINT
USER
PRINT
TEST
SAVE
TEST
DNA (260/280)
260.0 nm
280.0 nm
OFF
50.00
0.000
NO
1.00
µg/ml
0
OFF
STORED
TESTS
VIEW
RESULTS
DNA (260/280) test parameters
Measuring DNA
1. Display the appropriate DNA parameter screen and enter the initial
sample number (ID#).
2. Place the blank in the cell holder.
3. Press Zero/Base to measure the blank.
4. Place the unknown sample in the cell holder.
5. Press Run to start the measurement.
The DNA measurement screen is displayed. When the instrument is
finished measuring the absorbance of the sample, it displays the
absorbance, DNA ratio and DNA concentration similar to the example
below.
Note
Use the arrow keys to display the next or previous page.
Spectrophotometer User Guide
57
TEST NAME: DNA (260/280)
ID#
ABS(λ1)
260.0 nm
ABS(λ2)
280.0 nm
1
0.123
DNA RATIO
DNA CONC
PROTEIN CONC
0.456
= 1.7
= 1234.5 µg/ml
= 1111.1 µg/ml
PRINT
LIST
SAVE
DATA
TEST
PAGE
CLEAR
RESULTS
DNA (260/280) test results
DNA with scan tests
This group includes two tests that function almost identically – the only
difference is in the wavelengths range used for the measurements (one
measures absorbance between 260 nm and 280 nm; the other between
260 nm and 230 nm.)
The example below shows the parameters for the DNA with scan
(260/280) test. For the DNA with scan (260/230) test, Wavelength 2 is set
to 230 nm.
* DNA WITH SCAN *
TEST NAME
WAVELENGTH 1
WAVELENGTH 2
REF. WAVELENGTH
CORRECTION
DNA FACTOR (λ1)
DNA FACTOR (λ2)
DISPLAY PROTEIN
DILUTION MULTIPLIER
UNITS
ID# (0=OFF)
AUTOPRINT
USER
SETUP
SCAN
PRINT
TEST
SAVE
TEST
DNA WITH SCAN
260.0 nm
280.0 nm
OFF
50.00
0.000
NO
1.00
µg/ml
0
OFF
STORED
TESTS
DNA with scan (260/280) test parameters
58
Spectrophotometer User Guide
VIEW
RESULTS
Measuring DNA using scans
1. Display the appropriate DNA parameter screen and enter the initial
sample number (ID#).
2. Place the blank in the cell holder.
3. Press Zero/Base to measure the blank.
4. Place the unknown sample in the cell holder.
5. Press Run to start the measurement.
The DNA measurement screen is displayed. The instrument scans the
sample and displays a graph of the scan along with the sample ID#,
DNA ratio, DNA concentration and protein concentration. Here is an
example.
TEST NAME: DNA WITH SCAN
Graph of scan with data at 260 nm and
280 nm marked (if Ref wl = On)
ID#
99
DNA RATIO
1.7
RESCALE
PRINT
ALL
DNA
254.6 µg/ml
SAVE
DATA
TEST
PAGE
PROTEIN
18.1 µg/ml
CLEAR
RESULTS
DNA with scan test results
Direct UV measurements of nucleic acids
This group includes:
•
•
•
DNA/RNA tests
RNA tests
Oligonucleotide tests using an entered factor
Spectrophotometer User Guide
59
All of these tests use similar test parameters. For example, the Factor used
to convert absorbance to concentration for the direct UV measurement of
ssDNA is 50 (see the example below). The Factor is 40 for the direct UV
measurement of RNA and 33 for the direct UV measurement of
oligonucleotides.
Direct UV measurement of dsDNA test parameters
Running Direct UV measurements of nucleic acids
1. Display the appropriate Direct UV parameter screen and enter the
initial sample number (ID#).
2. Place the blank in the cell holder.
3. Press Zero/Base to measure the blank.
4. Place the unknown sample in the cell holder.
5. Press Run to start the measurement.
The Direct UV measurement screen is displayed. When the instrument
is finished measuring the absorbance of the sample, it displays the ID#,
absorbance and concentration. Here is an example:
60
Spectrophotometer User Guide
Direct UV measurement of ssDNA test results
Oligonucleotide measurement – calculated factor
The Oligonucleotide test with a calculated factor measures absorbance at
260 nm and uses a calculated factor to convert absorbance to
concentration. The instrument uses the molecular weight and the
extinction coefficient to calculate the factor.
* OLIGOS (CALC) *
TEST NAME
WAVELENGTH
DILUTION MULTIPLIER
UNITS
ID# (0=OFF)
AUTOPRINT
OLIGOS (CALC)
260.0 nm
1.00
µg/ml, pmol/µl
0
OFF
BASE SEQUENCE =
ATCGTCGATTGAGCATCAGCATGACTAGAGATCAGAA
TCGCG
BASE SEQUENCE FACTOR
26.68
BASE
SEQ.
PRINT
TEST
SAVE
TEST
STORED
TESTS
VIEW
RESULTS
Oligonucleotide test parameters (calculated factor)
Spectrophotometer User Guide
61
Measuring oligonucleotides using a calculated factor
1. Enter a base sequence.
With the Oligos (calc factor) parameter screen displayed, press Base
Seq. to view the Base Sequence screen and then follow the instructions
in the Oligo calculator functions section of this manual to specify the
sequence.
2. Enter the initial sample number (ID#).
3. Place the blank in the cell holder.
4. Press Zero/Base to measure the blank.
5. Place the unknown sample in the cell holder.
6. Press Run to start the measurement.
The Oligos (calc factor) measurement screen is displayed. When the
instrument is finished measuring the absorbance of the sample, it
displays the ID#, absorbance and concentration. Here is an example:
Note
Use the arrow keys to display the next or previous page.
TEST NAME: OLIGOS (CALC)
ID#
ABS(λ1)
260.0 nm
OLIGOS
µg/ml
OLIGOS
pmol/µl
999
1
2
3
-0.123
0.345
0.678
1.234
123.4
12.34
1.234
12345
56.7
5.67
0.567
578980
PRINT
LIST
SAVE
DATA
TEST
PAGE
CLEAR
RESULTS
Oligonucleotide test results (calculated factor)
62
Spectrophotometer User Guide
Protein measurements – Standard Curve method
These tests can be used to determine the concentration of protein in an
unknown sample based on any of the following analytical methods:
Coomassie/Bradford Standard or Coomassie/Bradford Micro –
Measures absorbance at 595 nm.
Lowry – Measures absorbance at 550 nm.
Pierce-Modified Lowry – Measures absorbance at 750 nm.
BCA (Bicinchoninic Acid) Standard or Pierce Micro BCA – Measures
absorbance at 562 nm.
Biuret - Measures absorbance at 540 nm.
Many of these tests use similar parameters. The example below shows the
parameters for the standard Bradford test. For a list of all parameters and
calculations for each test, refer to Appendices A and B.
* COOMASSIE/BRADFORD STANDARD *
TEST NAME
DATE STANDARDS
MEASURED
WAVELENGTH
CURVE FIT
STANDARDS
UNITS
ID# (0=OFF)
LOW/HIGH LIMITS
STATISTICS
AUTOPRINT
USER
CALIB
RATE
PRINT
TEST
Coomassie/Bradford
260.0 nm
595.0 nm
QUADRATIC
8
µg/ml
0
-9999/9999
OFF
OFF
SAVE
TEST
STORED
TESTS
VIEW
RESULTS
Coomassie/Bradford Standard test parameters
Preparing and running a standard curve
1. Display the appropriate protein parameter screen and then select
Standards and press Enter.
2. Edit any changed concentration values and press Accept.
Spectrophotometer User Guide
63
3. When all the parameters are correct, press Calibrate.
4. Follow the on-screen instructions to start the calibration by
inserting a blank or standard at each prompt and pressing Run.
After the instrument has measured the last standard, the Calibration
screen shows the absorbance of each standard along with the equation
of the calculated standard curve.
Editing a standard curve
You may re-measure any standard on a standard curve, delete specific
points from a curve or select a different curve fit.
To edit a standard:
1. Press Standards on the Calibration screen.
2. Press Edit Std on the Standards Results screen.
3. Select the standard you want to edit by entering its number in the
pop-up entry box.
A pop-up menu appears. Select the option to remove the standard from
the calibration, restore a previously ignored value or remeasure the
standard’s absorbance and follow the on-screen instructions.
To select a different fit for a standard curve:
1. Press Standards on the Calibration screen
2. Press Edit Curve on the Standards Results screen.
3. Select a fit for the standard curve and press Enter.
The instrument applies the selected curve fit to the data and displays
the new calibration equation and coefficient.
Measuring protein
1. Place the blank in the cell holder.
2. With the appropriate protein parameter screen displayed, press
Zero/Base to measure the blank.
64
Spectrophotometer User Guide
3. Place the unknown sample in the cell holder.
4. Press Run.
The protein measurement screen is displayed and the measurement
starts. When the instrument is finished measuring the absorbance of
the sample, it displays the ID#, absorbance and concentration. Here is
an example:
Note
Use the arrow keys to display the next or previous page.
TEST NAME: BRADFORD-STD
ID#
ABS(λ1)
260.0 nm
CONC
µg/ml
999
1
2
0.121
0.234
0.345
123.45
2345.6
345678
PRINT
LIST
SAVE
DATA
TEST
PAGE
CLEAR
RESULTS
Coomassie/Bradford Standard test results
Direct UV measurements of proteins
The Direct UV method determines protein concentration based on
absorbance at 280 nm or 205 nm.
The example below shows the parameters for the Direct UV protein test at
280 nm. For the Direct UV test at 205 nm, Wavelength is set to 205 nm.
Spectrophotometer User Guide
65
Running Direct UV measurements of proteins
1. With the appropriate Direct UV parameter screen displayed, enter
the initial sample number (ID#).
2. Place the blank in the cell holder.
3. Press Zero/Base to measure the blank.
4. Place the unknown sample in the cell holder.
5. Press Run to start the measurement.
When the instrument is finished measuring the absorbance of the
sample, it displays the ID#, absorbance and concentration. Here is an
example:
66
Spectrophotometer User Guide
Warburg-Christian test
The Warburg Christian test uses an absorbance difference measurement (at
280 and 260 nm) to determine the concentration of protein in an
unknown sample.
* WARBURG-CHRISTIAN *
TEST NAME
WAVELENGTH 1
WAVELENGTH 2
PROTEIN FACTOR (λ1)
PROTEIN FACTOR (λ2)
DILUTION MULTIPLIER
UNITS
ID# (0=OFF)
LOW/HIGH LIMITS
STATISTICS
AUTOPRINT
USER
PRINT
TEST
SAVE
TEST
WARBURG-CHRISTIAN
280.0 nm
260.0 nm
1.550
0.760
1.00
mg/ml
0
-9999/9999
OFF
OFF
STORED
TESTS
VIEW
RESULTS
Warburg-Christian test parameters
Running the Warburg-Christian test
1. With the Warburg-Christian parameter screen displayed, enter the
initial sample number (ID#).
2. Place the blank in the cell holder.
3. Press Zero/Base to measure the blank.
4. Place the unknown sample in the cell holder.
5. Press Run to start the measurement.
When the instrument is finished measuring the absorbance of the
sample, it displays the sample ID#, its absorbance at 280 and 260 nm
and its protein concentration. Here is an example:
Spectrophotometer User Guide
67
TEST NAME: WARBURG-CHRISTIAN
ID#
ABS(λ1)
280.0 nm
999
0.123
0.456
PROTEIN CONC
= 1111.1 mg/ml
1.234
1.567
PROTEIN CONC
= 33.8
mg/ml
1
PRINT
LIST
SAVE
DATA
ABS(λ2)
260.0 nm
TEST
PAGE
CLEAR
RESULTS
Warburg-Christian test results
Cell growth test
The cell growth test uses absorbance at 600 nm to indicate the progress of
cell growth in a sample. The instrument does not perform any calculations
or graphing for the data.
* CELL GROWTH *
TEST NAME
WAVELENGTH
SAMPLE POSITIONER
ID# (0=OFF)
LOW/HIGH LIMITS
STATISTICS
AUTOPRINT
USER
PRINT
TEST
SAVE
TEST
CELL GROWTH
600.00 nm
OFF
0
-9999/9999
OFF
OFF
STORED
TESTS
VIEW
RESULTS
Cell growth test parameters
Measuring cell growth
1. Display the cell growth parameter screen and enter the initial
sample number (ID#).
2. Place the blank in the cell holder.
3. Press Zero/Base to measure the blank.
4. Place the unknown sample in the cell holder.
68
Spectrophotometer User Guide
5. Press Run to start the measurement.
When the instrument is finished measuring the absorbance of the
sample, it displays the sample number and absorbance on the screen.
Oligo calculator functions
The oligonucleotide calculator determines the following data for a base
sequence that you enter:
•
•
•
•
•
•
Number of bases
Percent GC content
Molecular weight
Absorptivity (ε)
Conversion factor to be used in Oligonucleotide measurements
Tm for oligos up to 20-mers, DNA-DNA hybrids, DNA-RNA hybrids
and RNA-RNA hybrids
Specifying a base sequence
You must enter a base sequence before you can run the oligonucleotide
calculations.
1. Display the Base Sequence screen and select the required base.
2. Press Enter to add the base to the sequence.
Repeat these steps until you have specified the entire base sequence. The
displayed number of bases, percent GC content, molecular weight,
absorptivity (ε), and conversion factor will be updated as each new base is
added to the sequence.
Using the oligonucleotide calculator
To view the Melting Point calculator, display the Base Sequence screen and
press Tm Calc. Set the parameters as desired (see Parameter Entry for
details).
When you have finished setting parameters, the relevant set of melting
point predictions is displayed.
Spectrophotometer User Guide
69
AquaMate Methods
This section applies to the AquaMate spectrophotometers only.
The AquaMate models include a variety of methods to measure specific
compounds. The methods determine sample concentration by measuring
sample absorbance and then comparing to standards measured at fixed
wavelength locations (i.e., the Fixed application) or a concentration curve
(i.e., the Quant application).
The tests are provided on a USB memory device. Descriptions of the tests,
including the analyte, measurement range, program and file number, are
provided at the end of this section.
Spectrophotometer User Guide
70
How to run an AquaMate method
This section gives general information about:
•
How to load one of the provided AquaMate methods
•
How to save a method to the Library
•
The method results
For information about running a specific method, such as one of the Hach
or Merck® methods, see the chapter with that name below.
Loading a method
To load an AquaMate method from the Methods USB memory device:
1. Insert the device into the drive.
2. Display the Home screen and select USB MEM.
3. Select Load and press Enter.
To run an AquaMate method from the Library:
1. Display the Home screen and select Library.
2. Select a test and press Enter.
After you select a test, the Fixed or Quant screen appears, depending on the
file type of the selected file (.QNT or .FXD). The file name of the selected
test appears at the top of the screen.
Spectrophotometer User Guide
71
Saving a method to the Library
To save a method to the Library, select Save To Library.
Note
You can delete the 20 preinstalled methods found in the library to make
space for your preferred methods. These 20 methods are also included on
the Merck/Hach methods USB memory device in case you need them in
the future.
About the method results
Each time you use an AquaMate method to measure a sample, the Fixed
Results or Quant Results screen appears (depending on the file type of the
method file) with the following information:
•
•
•
absorbance of the sample
concentration of the analyte
Pass/Fail result.
The Pass/Fail result indicates whether the recorded concentration of the
sample falls within the measurement range of the test. The Pass/Fail result
has three possible states:
72
Spectrophotometer User Guide
Result
Meaning
Solution
Pass
Analyte concentration of the sample
is within the measurement range of
the selected test.
Save the result.
Fail
Analyte concentration in the sample
is too low.
Select a different method with an
appropriate range.
FAIL
Analyte concentration is too high.
Select a different method with an
appropriate range, or dilute the
sample to fit within the
measurement range of this method.
Disk 1 - Merck Spectroquant® methods
All the Merck Spectroquant methods are Fixed (.FXD) files, which measure
sample absorbance values at fixed wavelengths and compare them to known
standards measured at the same locations. The majority of files have the
following format:
The methods determine sample concentration by measuring sample
absorbance and then comparing to standards measured at fixed wavelength
locations (i.e., the Fixed application) or a concentration curve (i.e., the
Quant application).
14xxxPyy.FXD
where:
Note
14xxx
=
Merck Catalog Number
yy
=
Pathlength of cell in nm
FXD
=
Fixed application in software
In some cases, the Merck catalog number is of the format 10xxxx. In this
case the AquaMate files have the format “0xxxxPyy.FXD”.
Operation
Prepare the sample and blank according to the instructions supplied with
the test kit.
1. Load the method (see Loading a method for details).
2. Place the blank in the cell holder and press Zero Base to measure
the baseline.
3. Insert the prepared sample into the cell holder and press Run to
measure the sample.
The Fixed Results screen appears.
4. To measure another sample, insert the sample and press Run.
Spectrophotometer User Guide
73
Test results
In all but one method (14825P50.FXD) the relationship between
absorbance and concentration is linear over the specified measurement
range and takes the general form:
C = A x FACTOR
Therefore, the UVcalc equation typically takes the following form:
Method: 14566P16.FXD
Equation:
Zinc
M1*4.88
The factors entered are those documented by Merck; however, the values of
these factors may be affected by local conditions. In all cases, we
recommend that you check the factors using standard solutions appropriate
to your laboratory and modify the equation accordingly.
Note that the Merck methods do not use timers. You can easily add them,
if desired, by referring to the Timer parameter description in the Fixed
section of this manual.
74
Spectrophotometer User Guide
Disk 2 – Hach test kit methods
The Hach test kit includes two types of method files:
•
.FXD (measures absorbance at up to 20 fixed wavelengths)
•
.QNT (compares measured absorbance values against a concentration
curve to determine sample concentration).
Instructions for running the Hach methods on the AquaMate are stored in
PDF format on this CD.
.FXD method files
The .FXD files have the following format:
Hxxxx.FXD
where:
xxxx
=
Hach program number
FXD
=
Fixed application in software
If the Hach method indicates that timers are required, then a number
between 1 and 4 will appear next to the Timer(s) option on the Fixed
screen.
Operation
Load the method (see Loading a method for instructions). Follow the Hach
procedure until the first timer is required. Then continue with the steps
below:
1. Press Run to start the first timer.
Note
Some procedures require a zero measurement before the timer sequence is
activated. In this case, insert the blank and press Zero Base to measure the
baseline. Then press Run to activate the first timer.
The screen shows the action to be carried out and the time remaining.
Here is an example:
SHAKE
REMAINING TIME
:
02.46
Spectrophotometer User Guide
75
The instrument beeps to indicate the end of the time period and a
Timer Finished menu box is displayed with the following options:
•
•
•
Proceed (starts the next timer)
Zero (takes a baseline measurement)
Stop (cancels the current operation)
2. When you are ready to start the next timer, select Proceed and press
Enter.
At the end of this timer, the same Timer Finished menu box is
displayed.
3. If the method requires a zero measurement, insert the blank, select
Zero and press Enter.
The instrument measures the baseline and displays a menu box with
options to Proceed or Stop.
4. Insert the sample into the cell holder and select Proceed.
The instrument measures the sample and displays the Fixed Results
screen.
5. To measure another sample, insert the sample and press Run.
Test results
In all cases the relationship between absorbance and concentration takes
the general form:
C = A x FACTOR
Therefore, the UVcalc equation typically takes the following form:
Method:
Equation:
H1310.FXD
Bromine
M1 2.25
The factors entered are generic. In all cases, we recommend that you check
the factors with standard solutions appropriate to your laboratory and
modify the equation accordingly.
76
Spectrophotometer User Guide
.QNT method files
The .QNT files have the following format:
Hxxxx.QNT
where:
xxxx
=
Hach program number
QNT
=
Quant application in software
QNT files are set up for methods that require a calibration graph for each
new batch of reagent.
Calibration
Calibrations have been prepared for most of the Hach Quant methods.
These methods are ready for use as soon as they have been loaded.
However, these calibrations may be affected by local conditions. In all
cases, we recommend that you recalibrate with standard solutions
appropriate to your laboratory and store the method with a new file name.
In a few cases, new calibrations are required for each reagent batch or
plating bath formulation. You must calibrate these methods before you use
them.
General instructions for performing a calibration follow below. Specific
instructions and details of standard preparation are included in the PDF
file for the method.
•
To view the standards to be prepared, select Standards from the Quant
screen and press Enter. Compare these standards to those detailed on
the Hach procedure sheet. If the preparation of standards requires the
same timers as the samples, run the timers by selecting the Timer(s)
option from the Quant screen and pressing Run Timers.
•
When the standards are ready for measurement, press Calibrate. Follow
the on-screen instructions to measure the standards. After all standards
have been measured, the calibration graph is displayed along with the
coefficient.
•
Make sure you save the method file to the Library or USB memory
device by pressing Save Method. The program is now ready to use for
measuring samples.
Spectrophotometer User Guide
77
Operation
Load the method (see Loading a method for instructions). Follow the Hach
procedure until the first timer is required. Then continue with the steps
below:
1. Press Run to start the first timer.
Note
Some procedures require a zero measurement before the timer sequence is
activated. In this case, insert the blank and press Zero Base to measure the
baseline. Then press Run to activate the first timer.
The screen shows the action to be carried out and the time remaining.
Here is an example:
SHAKE
REMAINING TIME
:
02.46
The instrument beeps to indicate the end of the time period and a
Timer Finished menu box is displayed with the following options:
•
•
•
Proceed (starts the next timer)
Zero (takes a baseline measurement)
Stop (cancels the operation)
2. To start the next timer, select Proceed and press Enter.
At the end of this timer, the same Timer Finished menu box is
displayed.
3. If the method requires a zero measurement, insert the blank, select
Zero and press Enter.
The instrument measures the baseline and displays a menu box with
options to Proceed or Stop.
4. Insert the sample into the cell holder and select Proceed.
The instrument measures the sample and displays the Fixed Results
screen.
5. To run another sample, insert the sample and press Run.
78
Spectrophotometer User Guide
Test results
The Quant methods automatically take measurements from the calibration
graph in concentration units. The UVcalc equation is therefore of the form:
Method:
H1260.QNT
Equation:
Boron
M1
In effect, the UVcalc equation is used to indicate the chemical form and set
the measuring range limits.
Spectrophotometer User Guide
79
Disk 3 – Dr. Lange cuvette and pipette test kit methods
All the Dr. Lange cuvette and pipette tests are Fixed (.FXD) methods.
These files have the following format:
Kxxxyyy.FXD
Wxxxyyy.FXD
where:
Kxxx or Wxxx
=
Last four digits of the Lange test kit
FXD
=
Fixed application in software
Operation
Prepare the sample and blank according to the instructions supplied with
the test kit.
1. Load the method (see Loading a method for details).
The Fixed screen is displayed with the method file name at the top.
2. Place the blank in the cell holder and press Zero Base to measure
the baseline.
3. Insert the prepared sample into the cell holder and press Run.
The instrument measures the sample and displays the Fixed Results
screen.
4. To measure another sample, insert the sample and press Run.
Test results
In all methods, the relationship between absorbance and concentration is
linear and takes the general form:
C = A x FACTOR
Therefore, the UVcalc equation typically takes the following form:
Method: K307CT.FXD
Equation:
80
Spectrophotometer User Guide
M1 1.74
Boron
The factors entered are those documented by Dr Lange. However, the
values of these factors may be affected by local conditions. In all cases, we
recommend that you check the factors using standard solutions appropriate
to your laboratory and modify the equation accordingly.
Note that timers are not incorporated into the Dr. Lange methods. You
can easily add them, if desired, by referring to the Timer parameter
description in the Fixed section of this manual.
Spectrophotometer User Guide
81
Disk 4 – CHEMetrics Vacu Vial methods
All the CHEMetrics® Vacu-Vial® methods are Fixed (.FXD) files, which
measure sample absorbance values at fixed wavelengths and compare them
to known standards measured at the same locations. . These files have the
following format:
Cxxxx.FXD
Operation
Prepare the sample and blank according to the instructions supplied with
the test kit.
1. Load the method (see Loading a method for details).
2. Place the blank in the cell holder and press Zero Base to take a
baseline measurement.
3. Insert the prepared sample into the cell holder and press Run to
measure the sample.
4. To measure another sample, insert the sample and press Run.
Test results
In all of these methods, the relationship between absorbance and
concentration is linear and takes the general form:
C = A x FACTOR + INTERCEPT
Therefore, the UVcalc equation typically takes the following form:
METHOD: C1603.FXD
Bromine
EQUATION: M1*7.89 + 0.04
CHEMetrics determines the Factor and Intercept values specifically for the
AquaMate. However, the values of these factors may be affected by local
conditions. In all cases, we recommend that you check the factors using
standard solutions appropriate to your laboratory and modify the equation
accordingly.
82
Spectrophotometer User Guide
Note that the CHEMetrics methods do not use timers. You can easily add
them, if desired, by referring to the Timer parameter description in the
Fixed section of this manual.
Spectrophotometer User Guide
83
AquaMate method descriptions
This section describes the AquaMate methods in each test kit, including
the analyte, test description, type of cell used (when applicable),
measurement range, the manufacturer’s program number and file name,
Merck
Analyte
Range
Cell Type
Units
Program
AquaMate File Name
Alcohol
0.40 - 5.00 g/l
16 mm Round
g/l Alco
14965
14965P16.FXD
0.40 - 5.00 g/l
10 mm Rectangular g/l Alco
14965
14965P10.FXD
0.02 - 1.50 mg/l
10 mm Rectangular mg/l Al
14825
14825P10.FXD
0.05 - 0.75 mg/l
20 mm Rectangular mg/l Al
14825
14825P20.FXD
0.05 - 0.35 mg/l
50 mm Rectangular mg/l Al
14825
14825P50.FXD
Boron
0.050 - 0.800 mg/l 10 mm Rectangular mg/l B
14839
14839P10.FXD
Cadmium
0.025 - 1.000 mg/l 16 mm Round
mg/l Cd
14834
14834P16.FXD
0.025 - 1.000 mg/l 20 mm Rectangular mg/l Cd
14834
14834P20.FXD
0.025 - 1.000 mg/l 10 mm Rectangular mg/l Cd
14834
14834P10.FXD
0.010 - 0.300 mg/l 50 mm Rectangular mg/l Cd
14834
14834P50.FXD
5 - 80 mg/l
20 mm Rectangular mg/l Ca
14815
14815P20.FXD
10 - 160 mg/l
1.0 - 15.0 mg/l*
10 mm Rectangular mg/l Ca
mg/l Ca
14815
14815P10.FXD
5 - 125 mg/l
16 mm Round
mg/l Cl-
14730
14730P16.FXD
5 - 125 mg/l
20 mm Rectangular mg/l Cl-
14730
14730P20.FXD
5 - 125 mg/l
10 mm Rectangular mg/l Cl-
14730
14730P10.FXD
0.01 - 1.50 mg/l
50 mm Rectangular mg/l Cl2
14828
14828P50.FXD
0.05 - 4.00 mg/l
20 mm Rectangular mg/l Cl2
14828
14828P20.FXD
0.10 - 7.50 mg/l
10 mm Rectangular mg/l Cl2
14828
14828P10.FXD
Aluminum
Calcium
Chloride
Chlorine
84
Spectrophotometer User Guide
Analyte
Range
Cell Type
Units
Program
AquaMate File Name
Chlorine, Chlorine Dioxide 0.01 - 1.00 mg/l
& Ozone
0.02 - 1.00 mg/l
0.01 - 1.00 mg/l
50 mm Rectangular mg/l Cl2
mg/l ClO2
mg/l O3
14732
14732P50.FXD
0.05 - 2.50 mg/l
0.05 - 2.50 mg/l
0.05 - 2.50 mg/l
20 mm Rectangular mg/l Cl2
mg/l ClO2
mg/l O3
14732
14732P20.FXD
0.10 - 5.00 mg/l
0.10 - 5.00 mg/l
0.10 - 5.00 mg/l
10 mm Rectangular mg/l Cl2
mg/l ClO2
mg/l O3
14732
14732P10.FXD
0.05 - 2.00 mg/l
16 mm Round
mg/l Cr
14552
14552P16.FXD
0.05 - 2.00 mg/l
20 mm Rectangular mg/l Cr
14552
14552P20.FXD
0.05 - 2.00 mg/l
10 mm Rectangular mg/l Cr
14552
14552P10.FXD
0.010 - 0.600 mg/l 50 mm Rectangular mg/l Cr
14758
14758P50.FXD
0.03 - 1.50 mg/l
20 mm Rectangular mg/l Cr
14758
14758P20.FXD
0.05 - 3.00 mg/l
10 mm Rectangular mg/l Cr
14758
14758P10.FXD
4.0 - 40.0 mg/l
16 mm Round
mg/l COD
14560
14560P16.FXD
10 - 150 mg/l
16 mm Round
mg/l COD
14540
14540P16.FXD
15 - 300 mg/l
16 mm Round
mg/l COD
14895
14895P16.FXD
50 - 500 mg/l
16 mm Round
mg/l COD
14690
14690P16.FXD
100 - 1500 mg/l
16 mm Round
mg/l COD
14541
14541P16.FXD
300 - 3500 mg/l
16 mm Round
mg/l COD
14691
14691P16.FXD
500 - 10000 mg/l
16 mm Round
mg/l COD
14555
14555P16.FXD
0.10 - 8.00 mg/l
16 mm Round
mg/l Cu
14553
14553P16.FXD
0.05 - 3.00 mg/l
20 mm Rectangular mg/l Cu
14553
14553P20.FXD
0.10 - 6.00 mg/l
10 mm Rectangular mg/l Cu
14553
14553P10.FXD
0.02 - 1.20 mg/l
50 mm Rectangular mg/l Cu
14767
14767P50.FXD
0.05 - 3.00 mg/l
20 mm Rectangular mg/l Cu
14767
14767P20.FXD
0.10 - 6.00 mg/l
10 mm Rectangular mg/l Cu
14767
14767P10.FXD
Chromium
COD, Oxygen Demand,
Chemical
Copper
Spectrophotometer User Guide
85
Analyte
Range
Cyanide
0.010 - 0.500 mg/l 16 mm Round
Fluoride
Formaldehyde
Gold
Units
Program
AquaMate File Name
mg/l CN-
14561
14561P16.FXD
0.005 - 0.250 mg/l 20 mm Rectangular mg/l CN-
14561
14561P20.FXD
0.010 - 0.500 mg/l 10 mm Rectangular mg/l CN-
14561
14561P10.FXD
0.002 - 0.100 mg/l 50 mm Rectangular mg/l CN-
14800
14800P50.FXD
0.005 - 0.250 mg/l 20 mm Rectangular mg/l CN-
14800
14800P20.FXD
0.010 - 0.500 mg/l 10 mm Rectangular mg/l CN-
14800
14800P10.FXD
0.002 - 0.100 mg/l 50 mm Rectangular mg/l CN-
109701
09701P50.FXD
0.005 - 0.250 mg/l 20 mm Rectangular mg/l CN-
109701
09701P20.FXD
0.010 - 0.500 mg/l 10 mm Rectangular mg/l CN-
109701
09701P10.FXD
0.10 - 1.50 mg/l
16 mm Round
mg/l F-
14557
14557P16.FXD
0.10 - 1.50 mg/l
20 mm Rectangular mg/l F-
14557
14556P20.FXD
0.10 - 1.50 mg/l
10 mm Rectangular mg/l F-
14557
14557P10.FXD
0.025 - 0.500 mg/l 50 mm Rectangular mg/l F-
14557
14557P50.FXD
0.1 - 10.0 mg/l
16 mm Round
mg/l HCHO
14500
14500P16.FXD
0.05 - 6.00 mg/l
20 mm Rectangular mg/l HCHO
14500
14500P20.FXD
0.1 - 10.0 mg/l
10 mm Rectangular mg/l HCHO
14500
14500P10.FXD
0.02 - 1.50 mg/l
50 mm Rectangular mg/l HCHO
14678
14678P50.FXD
0.05 - 4.00 mg/l
20 mm Rectangular mg/l HCHO
14678
14678P20.FXD
1.00 - 9.00 mg/l
10 mm Rectangular mg/l HCHO
14678
14678P10.FXD
0.5 - 12.0 mg/l
10 mm Rectangular mg/l Au
14821
14821P10.FXD
16 mm Round
mg/l Ca
14683
14683P16.FXD
0.25 - 2.50 mg/l
20 mm Rectangular mg/l Ca
14683
14683P20.FXD
0.50 - 5.00 mg/l
10 mm Rectangular mg/l Ca
14683
14683P10.FXD
Hardness, Residual
86
Cell Type
Spectrophotometer User Guide
Analyte
Range
Cell Type
Units
Program
AquaMate File Name
Hardness, Total
5 - 150 mg/l
16 mm Round
mg/l Ca
14565
14565P16.FXD
5 - 100 mg/l
20 mm Rectangular mg/l Ca
14565
14565P20.FXD
5 - 150 mg/l
10 mm Rectangular mg/l Ca
14565
14565P10.FXD
0.02 - 1.00 mg/l
50 mm Rectangular mg/l N2H4
14797
14797P50.FXD
0.10 - 2.50 mg/l
20 mm Rectangular mg/l N2H4
14797
14797P20.FXD
0.20 - 5.00 mg/l
10 mm Rectangular mg/l N2H4
14797
14797P10.FXD
0.005 - 0.400 mg/l 50 mm Rectangular mg/l N2H4
109711
09711P50.FXD
0.01 - 1.00 mg/l
20 mm Rectangular mg/l N2H4
109711
09711P20.FXD
0.02 - 2.00 mg/l
10 mm Rectangular mg/l N2H4
109711
09711P10.FXD
2.0 - 20.0 mg/l
16 mm Round
mg/l H2O2
14731
14731P16.FXD
0.25 - 5.00 mg/l
50 mm Rectangular mg/l H2O2
14731
14731P50.FXD
2.0 - 20.0 mg/l
10 mm Rectangular mg/l H2O2
14731
14731P10.FXD
0.05 - 4.00 mg/l
16 mm Round
mg/l Fe
14549
14549P16.FXD
0.03 - 2.50 mg/l
20 mm Rectangular mg/l Fe
14549
14549P20.FXD
0.05 - 5.00 mg/l
10 mm Rectangular mg/l Fe
14549
14549P10.FXD
0.005 - 1.000 mg/l 50 mm Rectangular mg/l Fe
14761
14761P50.FXD
0.03 - 2.50 mg/l
20 mm Rectangular mg/l Fe
14761
14761P20.FXD
0.05 - 5.00 mg/l
10 mm Rectangular mg/l Fe
14761
14761P10.FXD
1.0 - 50.0 mg/l
16 mm Round
mg/l Fe
14896
14696P16.FXD
1.0 - 50.1 mg/l
20 mm Rectangular mg/l Fe
14896
14896P20.FXD
1.0 - 50.0 mg/l
10 mm Rectangular mg/l Fe
14896
14896P10.FXD
0.10 - 5.00 mg/l
16 mm Round
mg/l Pb
14833
14833P16.FXD
0.10 - 5.00 mg/l
20 mm Rectangular mg/l Pb
14833
14833P20.FXD
0.10 - 5.00 mg/l
10 mm Rectangular mg/l Pb
14833
14833P10.FXD
5.0 - 50.0 mg/l
16 mm Round
mg/l Mg
14684
14684P16.FXD
5.0 - 50.0 mg/l
20 mm Rectangular mg/l Mg
14684
14684P20.FXD
5.0 - 50.0 mg/l
10 mm Rectangular mg/l Mg
14684
14684P10.FXD
Hydrazine
Hydrogen Peroxide
Iron
Lead
Magnesium
Spectrophotometer User Guide
87
Analyte
Range
Cell Type
Manganese
0.01 - 2.00 mg/l
Nickel
Nitrogen, Ammonia
88
Program
AquaMate File Name
50 mm Rectangular mg/l Mn
14770
14770P50.FXD
0.25 - 5.00 mg/l
20 mm Rectangular mg/l Mn
14770
14770P20.FXD
0.50 - 10.00 mg/l
10 mm Rectangular mg/l Mn
14770
14770P10.FXD
0.10 - 6.00 mg/l
16 mm Round
mg/l Ni
14554
14554P16.FXD
0.05 - 2.50 mg/l
20 mm Rectangular mg/l Ni
14554
14554P20.FXD
0.10 - 5.00 mg/l
10 mm Rectangular mg/l Ni
14554
14554P10.FXD
0.05 - 2.00 mg/l
50 mm Rectangular mg/l Ni
14785
14785P50.FXD
0.20 - 5.00 mg/l
20 mm Rectangular mg/l Ni
14785
14785P20.FXD
0.10 - 5.00 mg/l
10 mm Rectangular mg/l Ni
14785
14785P10.FXD
0.01 - 2.00 mg/l
0.01 - 2.60 mg/l
16 mm Round
mg/l NH4-N
mg/l NH4+
14739
14739P16.FXD
0.01 - 2.00 mg/l
0.01 - 2.60 mg/l
10mm Rectangular mg/l NH4-N
mg/l NH4+
14739
14739P10.FXD
0.20 - 8.00 mg/l
0.30 - 10.00 mg/l
16 mm Round
mg/l NH4-N
mg/l NH4+
14558
14558P16.FXD
0.20 - 8.00 mg/l
0.30 - 10.00 mg/l
10 mm Rectangular mg/l NH4-N
mg/l NH4+
14558
14558P10.FXD
0.5 - 16.0 mg/l
0.6 - 21.0 mg/l
16 mm Round
mg/l NH4-N
mg/l NH4+
14544
14544P16.FXD
0.5 - 16.0 mg/l
0.6 - 21.0 mg/l
10 mm Rectangular mg/l NH4-N
mg/l NH4+
14544
14544P10.FXD
4.0 - 80.0 mg/l
5.0 - 100.0 mg/l
16 mm Round
mg/l NH4-N
mg/l NH4+
14559
14559P16.FXD
4.0 - 80.0 mg/l
5.0 - 100.0 mg/l
10 mm Rectangular mg/l NH4-N
mg/l NH4+
14559
14559P10.FXD
0.010 - 0.500 mg/l 50 mm Rectangular mg/l NH4-N
mg/l NH4+
0.010 - 0.650 mg/l
14752
14752P50.FXD
0.03 - 1.50 mg/l
0.04 - 1.90 mg/l
20 mm Rectangular mg/l NH4-N
mg/l NH4+
14752
14752P20.FXD
0.05 - 3.00 mg/l
0.06 - 3.90 mg/l
10 mm Rectangular mg/l NH4-N
mg/l NH4+
14752
14752P10.FXD
Spectrophotometer User Guide
Units
Analyte
Range
Cell Type
Units
Program
AquaMate File Name
Nitrogen, Nitrate
0.11 - 3.40 mg/l
0.5 - 15.0 mg/l
16 mm Round
mg/l NO3-N
mg/l NO3-
14556
14556P16.FXD
0.05 - 1.50 mg/l
0.25 - 6.50 mg/l
20 mm Rectangular mg/l NO3-N
mg/l NO3-
14556
14556P20.FXD
0.10 - 3.00 mg/l
0.5 - 13.0 mg/l
10 mm Rectangular mg/l NO3-N
mg/l NO3-
14556
14556P10.FXD
1.0 - 50.0 mg/l
4 - 220 mg/l
16 mm Round
mg/l NO3-N
mg/l NO3-
14764
14764P16.FXD
1.0 - 50.0 mg/l
4 - 220 mg/l
10 mm Rectangular mg/l NO3-N
mg/l NO3-
14764
14764P10.FXD
0.5 - 18.0 mg/l
2.0 - 80.0 mg/l
16 mm Round
mg/l NO3-N
mg/l NO3-
14542
14542P16.FXD
0.02 - 10.0 mg/l
1.0 - 45.0 mg/l
20 mm Rectangular mg/l NO3-N
mg/l NO3-
14542
14542P20.FXD
0.5 - 20.0 mg/l
2.0 - 90.0 mg/l
10 mm Rectangular mg/l NO3-N
mg/l NO3-
14542
14542P10.FXD
0.5 - 25.0 mg/l
2 - 110 mg/l
16 mm Round
mg/l NO3-N
mg/l NO3-
14563
14563P16.FXD
0.25 - 12.5 mg/l
1.0 - 55.0 mg/l
20 mm Rectangular mg/l NO3-N
mg/l NO3-
14563
14563P20.FXD
0.5 - 25.0 mg/l
2 - 110 mg/l
10 mm Rectangular mg/l NO3-N
mg/l NO3-
14563
14563P10.FXD
0.2 - 10.0 mg/l
1.0 - 45.0 mg/l
20 mm Rectangular mg/l NO3-N
mg/l NO3-
14773
14773P20.FXD
0.5 - 20.0 mg/l
2.0 - 90.0 mg/l
10 mm Rectangular mg/l NO3-N
mg/l NO3-
14773
14773P10.FXD
mg/l NO2-N
mg/l NO2-
14547
14547P16.FXD
0.010 - 0.500 mg/l 20 mm Rectangular mg/l NO2-N
mg/l NO20.03 - 1.60 mg/l
14547
14547P20.FXD
0.020 - 1.000 mg/l 10 mm Rectangular mg/l NO2-N
mg/l NO20.100 - 3.00 mg/l
14547
14546P10.FXD
0.005 - 0.200 mg/l 50 mm Rectangular mg/l NO2-N
14776
14776P50.FXD
Nitrogen, Nitrite
0.020 - 0.610 mg/l 16 mm Round
0.05 - 2.00 mg/l
Spectrophotometer User Guide
89
Analyte
Range
Cell Type
Program
AquaMate File Name
0.010 - 0.500 mg/l 20 mm Rectangular mg/l NO2-N
mg/l NO20.03 - 1.60 mg/l
14776
14776P20.FXD
0.02 - 1.00 mg/l
0.10 - 3.00 mg/l
10 mm Rectangular mg/l NO2-N
mg/l NO2-
14776
14776P10.FXD
0.5 - 15.0 mg/l
16 mm Round
mg/l N
14537
14537P16.FXD
0.3 - 10.0 mg/l
20 mm Rectangular mg/l N
14537
14537P20.FXD
0.5 - 15.0 mg/l
10 mm Rectangular mg/l N
14537
14537P10.FXD
10 - 150 mg/l
16 mm Round
mg/l N
14763
14763P16.FXD
10 - 150 mg/l
10 mm Rectangular mg/l N
14763
14763P10.FXD
0.5 - 12.0 mg/l
16 mm Round
mg/l O2
14694
14694P16.FXD
0.5 - 12.0 mg/l
20 mm Rectangular mg/l O2
14694
14694P20.FXD
0.5 - 12.0 mg/l
10 mm Rectangular mg/l O2
14694
14694P10.FXD
0.10 - 2.50 mg/l
16 mm Round
mg/l phenol
14551
14551P16.FXD
0.025 - 1.000 mg/l 50 mm Rectangular mg/l phenol
14551
14551P50.FXD
0.10 - 2.50 mg/l
20 mm Rectangular mg/l phenol
14551
14551P20.FXD
0.10 - 2.50 mg/l
10 mm Rectangular mg/l phenol
14551
14551P10.FXD
0.01 - 1.00 mg/l
0.05 - 3.00 mg/l
50 mm Rectangular mg/l PO4-P
mg/l PO43-
14848
14848P50.FXD
0.03 - 2.50 mg/l
0.10 - 7.50 mg/l
20 mm Rectangular mg/l PO4-P
mg/l PO43-
14848
14848P20.FXD
0.05 - 5.00 mg/l
0.2 - 15.0 mg/l
10 mm Rectangular mg/l PO4-P
mg/l PO43-
14848
14848P10.FXD
0.05 - 5.00 mg/l
0.2 - 15.3 mg/l
16 mm Round
mg/l PO4-P
mg/l PO43-
14543
14543P16.FXD
0.03 - 2.50 mg/l
0.10 - 7.50 mg/l
20 mm Rectangular mg/l PO4-P
mg/l PO43-
14543
14543P20.FXD
0.05 - 5.00 mg/l
0.2 - 15.0 mg/l
10 mm Rectangular mg/l PO4-P
mg/l PO43-
14543
14543P10.FXD
0.5 -25 mg/l
16 mm Round
14729
14729P16.FXD
-
0.015 - 0.650 mg/l
Nitrogen, Total
Oxygen, Dissolved
Phenols
Phosphorus, PMB
90
Spectrophotometer User Guide
Units
mg/l NO2
mg/l PO4-P
Analyte
Range
Cell Type
Potassium
Silica
Silver
Sulphate
Sulphide
Program
AquaMate File Name
3-
1.5 - 75.0 mg/l
Phosphorus, VM
Units
mg/l PO4
0.5 -25 mg/l
1.5 - 75.0 mg/l
10 mm Rectangular mg/l PO4-P
mg/l PO43-
14729
14729P10.FXD
0.5 - 25.0 mg/l
1.5 - 75.0 mg/l
16 mm Round
mg/l PO4-P
mg/l PO43-
14546
14546P16.FXD
0.5 - 15.0 mg/l
1.5 - 45.0 mg/l
20 mm Rectangular mg/l PO4-P
mg/l PO43-
14546
14546P20.FXD
1.0 - 30.0 mg/l
3.0 - 90.0 mg/l
10 mm Rectangular mg/l PO4-P
mg/l PO43-
14546
14546P10.FXD
0.5 - 15.0 mg/l
1.5 - 45.0 mg/l
20 mm Rectangular mg/l PO4-P
mg/l PO43-
14842
14842P20.FXD
1.0 - 30.0 mg/l
3.0 - 90.0 mg/l
10 mm Rectangular mg/l PO4-P
mg/l PO43-
14842
14842P10.FXD
5.0 - 50.0 mg/l
16 mm Round
mg/l K
14562
14562P16.FXD
5.0 - 50.0 mg/l
20 mm Rectangular mg/l K
14562
14562P20.FXD
5.0 - 50.0 mg/l
10 mm Rectangular mg/l K
14562
14562P10.FXD
0.005 - 0.750 mg/l 50 mm Rectangular mg/l Si
14794
14794P50.FXD
0.05 - 2.50 mg/l
20 mm Rectangular mg/l Si
14794
14794P20.FXD
0.1 - 5.00 mg/l
10 mm Rectangular mg/l Si
14794
14794P10.FXD
0.25 - 1.50 mg/l
20 mm Rectangular mg/l Ag
14831
14831P20.FXD
0.50 - 3.00 mg/l
10 mm Rectangular mg/l Ag
14831
14831P10.FXD
5 - 250 mg/l
16 mm Round
mg/l SO42-
14548
14548P16.FXD
5 - 250 mg/l
20 mm Rectangular mg/l SO42-
14548
14548P20.FXD
5 - 250 mg/l
10 mm Rectangular mg/l SO42-
14548
14548P10.FXD
100 - 1000 mg/l
16 mm Round
mg/l SO42-
14564
14564P16.FXD
100 - 1000 mg/l
20 mm Rectangular mg/l SO42-
14564
14564P20.FXD
100 - 1000 mg/l
10 mm Rectangular mg/l SO42-
14564
14564P10.FXD
25 - 300 mg/l
10 mm Rectangular mg/l SO42-
14791
14791P10.FXD
14779
14779P50.FXD
0.020 - 0.500 mg/l 50 mm Rectangular mg/l S2-
Spectrophotometer User Guide
91
Analyte
Range
Cell Type
Sulphite
0.05 - 3.00 mg/l
Program
AquaMate File Name
50 mm Rectangular mg/l SO32-
14394
14394P50.FXD
0.5 - 15.0 mg/l
20 mm Rectangular mg/l SO32-
14394
14394P20.FXD
1.0 - 25.0 mg/l
10 mm Rectangular mg/l SO32-
14394
14394P10.FXD
1.0 - 25.0 mg/l
16 mm Round
mg/l SO32-
14394
14394P16.FXD
Surfactants Determination
0.05 - 2.0 mg/l
16 mm Round
mg/l MBAS
14697
14697P16.FXD
Tin
0.10 - 2.50 mg/l
16 mm Round
mg/l Sn
14622
14622P16.FXD
0.10 - 1.50 mg/l
20 mm Rectangular mg/l Sn
14622
14622P20.FXD
0.10 - 2.50 mg/l
10 mm Rectangular mg/l Sn
14622
14622P10.FXD
0.050 - 0.500 mg/l 50 mm Rectangular mg/l Zn
14566
14566P50.FXD
0.20 - 5.00 mg/l
20 mm Rectangular mg/l Zn
14566
14566P20.FXD
0.20 - 5.00 mg/l
10 mm Rectangular mg/l Zn
14566
14566P10.FXD
0.20 - 5.00 mg/l
16 mm Round
mg/l Zn
14566
14566P16.FXD
0.05 - 2.50 mg/l
10 mm Rectangular mg/l Zn
14832
14832P10.FXD
Zinc
92
Spectrophotometer User Guide
Units
Hach
Analyte
Method
Range
Program AquaMate File Name
Aluminum
Eriochrome Cyanine R
0 - 0.250 mg/l
1010
H1010.QNT
Aluminum
0 - 0.800 mg/l
1000
H1000.QNT
Arsenic
Silver Diethyldithiocarbamate
0 - 0.200 mg/l
1050
H1050.QNT
Barium
Turbidimetric
0 - 100 mg/l
1100
H1100.QNT
Turbidimetric (AccuVac)
0 - 100 mg/l
1110
H1110.QNT
Carmine
0 - 14.0 mg/l
1250
H1250.QNT
AzoMethine-H
0 - 1.5 mg/l
1260
H1260.QNT
DPD
0 - 4.50 mg/l
1300
H1300.FXD
DPD (AccuVac)
0 - 4.50 mg/l
1310
H1310.FXD
Cadmium
Dithizone
0 - 80 μg/l
1350
H1350.QNT
Chloride
Mercuric Thiocyanate
0 - 25.0 mg/l
1400
H1400.QNT
Chlorine, Free
DPD
0 - 2.00 mg/l
1450
H1450.FXD
DPD (AccuVac)
0 - 2.00 mg/l
1460
H1460.FXD
DPD
0 - 5.00 mg/l
1470
H1470.FXD
DPD (TNT)
0 - 5.00 mg/l
1480
H1480.FXD
DPD
0 - 2.00 mg/l
1450
H1450.FXD
DPD (AccuVac)
0 - 2.00 mg/l
1460
H1460.FXD
DPD
0 - 5.00 mg/l
1470
H1470.FXD
DPD (TNT)
0 - 5.00 mg/l
1480
H1480.FXD
Chlorophenol Red
0 - 1.00 mg/l
1500
H1500.FXD
Direct Reading
0 - 50 mg/l
1510
H1510.FXD
Direct Reading
0 - 1000 mg/l
1520
H1520.FXD
Chromium, Hexavalent 1,5-Diphenylcarbohydrazide
0 - 0.700 mg/l
1560
H1560.QNT
1,5-Diphenylcarbohydrazide
0 - 0.700mg/l
1570
H1570.QNT
Boron
Bromine
Chlorine, Total
Chlorine Dioxide
Spectrophotometer User Guide
93
Analyte
Method
Range
Program AquaMate File Name
Chromium, Total
Alkaline Hypobromite Oxidation
0 - 0.700 mg/l
1580
H1580.QNT
Chromium, Trivalent
Direct Reading
0 - 20.0 g/l
1550
H1550.FXD
Cobalt
PAN
0 - 2.00 mg/l
1600
H1600.QNT
COD, Oxygen Demand, Reactor Digestion
0 - 40 mg/l
2700
H2700.QNT
Chemical
Reactor Digestion
0 - 150 mg/l
2710
H2710.QNT
Reactor Digestion (Hg Free)
0 - 150 mg/l
Reactor Digestion
0 - 1500 mg/l
Reactor Digestion (Hg Free)
0 - 1500 mg/l
Reactor Digestion
0 - 15 g/l
2720
H2720+.QNT
Manganese III
20 - 1000mg/l
2730
H2730.QNT
Platinum-Cobalt
0 - 500 units
1670
H1670.QNT
Platinum-Cobalt
0 - 500 units
1680
H1680.QNT
Porphyrin
0 - 210.0 μg
1720
H1720.QNT
Bicinchoninate
0 - 5.000 mg/l
1700
H1700.QNT
Bicinchoninate (AccuVac)
0 - 5.000 mg/l
1710
H1710.QNT
Copper, Autocatalytic
Colorimetric
0 - 3.00 g/l
1690
H1690.QNT
Cyanide
Pyridine-Pyrazalone
0 - 0.240 mg/l
1750
H1750.QNT
Detergents, Anionic
Crystal Violet
0 - 0.275 mg/l
1850
H1850.QNT
Fluoride
SPADNS
0 - 2.00 mg/l
1900
H1900.QNT
SPADNS (AccuVac)
0 - 2.00 mg/l
1910
H1910.QNT
Formaldehyde
MBTH
0 - 500 μg/l
1950
H1950.QNT
Hardness
Chlorophosphonazo
0 - 1000 μg/l
2000
H2000.FXD
Hardness, Calcium or
Calmagite, Colorimetric
0 - 4.00 mg/l
2020
(Mg)
H2020.QNT
Color, True and
Apparent
Copper
94
Spectrophotometer User Guide
H2715.QNT
2720
H2720.QNT
H2725.QNT
Analyte
Method
Range
Magnesium
Program AquaMate File Name
2010 (Ca) H2010.QNT
0 - 600 μg/l
2050
H2050.QNT
p-Dimethylamino-benzaldehyde (AccuVac) 0 - 600 μg/l
2060
H2060.QNT
DPD
0 - 7.00 mg/l
2100
H2100.FXD
DPD (AccuVac)
0 - 7.00 mg/l
2110
H2110.FXD
FerroZine
0 - 1.400 mg/l
2175
H2175.QNT
FerroMo
0 - 1.800 mg/l
2160
H2160.QNT
TPTZ
0 - 1.800 mg/l
2190
H2190.QNT
TPTZ (AccuVac)
0 - 1.800 mg/l
2195
H2195.QNT
FerroVer
0 - 3.00 mg/l
2165
H2165.QNT
FerroVer (AccuVac)
0 - 3.00 mg/l
2170
H2170.QNT
1,10-Phenanthroline
0 - 3.00 mg/l
2150
H2150.QNT
1,10-Phenanthroline (AccuVac)
0 - 3.00 mg/l
2155
H2155.QNT
Fast Column Extraction (LeadTrak)
0 - 150 μg/l
2210
H2210.QNT
Dithizone
0 - 300 μg/l
2200
H2200.QNT
PAN
0 - 0.700 mg/l
2260
H2260.QNT
Periodate Oxidation
0 - 20.0 mg/l
2250
H2250.QNT
Ternary Complex
0 - 3.00 mg/l
2300
H2300.QNT
Mercaptoacetic Acid
0 - 50.0 mg/l
2310
H2310.QNT
Mercaptoacetic Acid (AccuVac)
0 - 50.0 mg/l
2320
H2320.QNT
Nickel
Heptoxime
0 - 1.80 mg/l
2360
H2360.QNT
Nickel, Autocatalytic
Photometric
0 - 8.00 g/l
2350
H2350.QNT
Nitrogen, Ammonia
Salicylate
0 - 0.80 mg/l
2455
H2455.QNT
Nessler (TNT)
0 - 2.50 mg/l
2400
H2400.QNT
Salicylate (TNT)
0 - 2.500 mg/l
2460
H2460.QNT
Salicylate (TNT)
0 - 50.0 mg/l
2465
H2465.QNT
Hydrazine
Iodine
Iron, Total
Iron, Ferrous
Lead
Manganese
Molybdenum,
Molybdate
p-Dimethylamino-benzaldehyde
Spectrophotometer User Guide
95
Analyte
Method
Range
Program AquaMate File Name
Nitrogen,
Monochloramine and
free ammonia
Salicylate (PP or AccuVac)
0 - 0.50 mg/l
2470
H2470.FXD
Nitrogen, Nitrate
Cadmium Reduction
0 - 0.50 mg/l
2515
H2515.QNT
Cadmium Reduction
0 - 5.0 mg/l
2520
H2520.QNT
Cadmium Reduction (AccuVac)
0 - 5.0 mg/l
2525
H2525.QNT
Cadmium Reduction
0 - 30.0 mg/l
2530
H2530.QNT
Cadmium Reduction (AccuVac)
0 - 30.0 mg/l
2535
H2535.QNT
Chromotropic Acid (TNT)
0 - 30.0 mg/l
2511
H2511.QNT
Diazotization
0-0.3000 mg/l
2610
H2610.FXD
Diazotization (AccuVac)
0-0.3000 mg/l
2620
H2620.FXD
Diazotization (TNT)
0-0.5000 mg/l
2630
H2630.FXD
Ferrous Sulphate
0 - 250 mg/l
2600
H2600.FXD
Titanium Reduction (TNT)
0 - 25.0 mg/l
2550
H2550.QNT
Nitrogen, Total Kjeldahl Nessler
0 - 150 mg/l
2410
H2410.QNT
Nitrogen, Total
Persulphate Digestion (TNT)
0 - 25 mg/l
2558
H2558.QNT
Palladium
N,N'-Dimethyldithiooxamide
0 - 250 mg/l
2850
H2850.QNT
Phenols
4-Aminoantipyrine
0 - 0.200 mg/l
2900
H2900.QNT
Phosphonates
Persulphate/UV Oxidation
0 - 2.50 to 0 125 mg/l
2950
H2950.QNT
Phosphorus, Reactive
PhosVer 3, Ascorbic Acid
0 - 2.500 mg/l
3025
H3025.QNT
PhosVer 3, (AccuVac)
0 - 2.500 mg/l
3030
H3030.QNT
PhosVer 3 (TNT)
0 - 5.00 mg/l
3035
H3035.QNT
Amino Acid
0 - 30.00 mg/l
3010
H3010.QNT
Molybdovanadate
0 - 45.00 mg/l
3015
H3015.QNT
Molybdovanadate (AccuVac)
0 - 45.00 mg/l
3020
H3020.QNT
PhosVer 3 (TNT)
0 - 3.50 mg/l
3036
H3036.QNT
Nitrogen, Nitrite
Nitrogen, Total
Inorganic
Phosphorus, Total
96
Spectrophotometer User Guide
Analyte
Method
Range
Program AquaMate File Name
Phosphorus, Acid
Hydrolyzable
Ascorbic Acid (TNT)
0 - 5.00 mg/l
3037
H3037.QNT
Platinum
N,N'-Dimethyldithiooxamide
0 - 10 g/l
3150
H3150.QNT
Potassium
Colorimetric
0 - 7.0 mg/l
3100
H3100.QNT
Quaternary Ammonium Direct Binary Complex
Compounds
0 - 5.00 mg/l
3200
H3200.QNT
Selenium
Diaminobenzidine
0 - 1.000 mg/l
3300
H3300.QNT
Silica
Heteropoly Blue
0 - 1.600 mg/l
3360
H3360.QNT
Silicomolybdate
0 - 100 mg/l
3350
H3350.QNT
Silver
Colorimetric
0 - 0.700 mg/l
3400
H3400.FXD
Sulphate
SulfaVer 4
0 - 70.0 mg/l
3450
H3450.QNT
SulfaVer 4 (AccuVac)
0 - 70.0 mg/l
3460
H3460.QNT
Sulphide
Methylene Blue
0 - 800 μg/l
3500
H3500.FXD
Tannin and Lignin
Tyrosine
0 - 9.0 mg/l
3550
H3550.QNT
Turbidity
Radiation Attenuation
0 - 5000 FAU
3750
H3750.QNT
Volatile Acid
Esterification
0 - 2800 mg/l
3800
H3800.QNT
Zinc
Zincon
0 - 3.000 mg/l
3850
H3850.QNT
Spectrophotometer User Guide
97
Lange
Units
Cell
Program
AquaMate File Name
BOD Oxygen demand, biological 0.5-12 mg/l
(5 day)
BOD5
11 mm Round
LCK554
K554CT.FXD
Carbonate/Carbon dioxide
55 - 550 mg/l
CO2
11mm Round
LCK 388 K388CT.FXD
Chloride
70-1000 mg/l
Cl-
11mm Round
LCK 311 K311CT.FXD
Chlorine, Total
0.05 - 1.5 mg/l
0.05 - 1.5 mg/l
mg/l Cl2
mg/l O3
11mm Round
LCW 510 W510RC.FXD
0.03 - 0.4 mg/l
0.03 - 0.4 mg/l
mg/l Cl2
mg/l O3
50 mm Rectangular
LCW 510 W510P50.FXD
0.03 - 1.0 mg/l
mg/l Cr
11 mm Round
LCK 313 K313CT.FXD
50 mm Rectangular
LCK 313 K313P50.FXD
Analyte
Chromium
Range
0.005 - 0.25 mg/l mg/l Cr
COD, Oxygen Demand,
15 - 150 mg/l
mg/l COD 11 mm Round
LCK 314 K314CT.FXD
Chemical
50 - 300 mg/l
mg/l COD 11 mm Round
LCK 614 K614CT.FXD
150 - 1000 mg/l mg/l COD 11 mm Round
LCK 114 K114CT.FXD
100 - 2000 mg/l mg/l COD 11 mm Round
LCK 514 K514CT.FXD
5 - 60 g/l
g/l COD
11 mm Round
LCK 914 K914CT.FXD
0.01 - 1.0 mg/l
mg/l COD
11 mm Round
LCK 529 K529CT.FXD
0.1 - 8.0 mg/l
mg/l COD
11 mm Round
LCK 329 K329CT.FXD
0.01 - 0.60 mg/l mg/l CN
11 mm Round
LCK 315 K315CT.FXD
0.01 - 0.60 mg/l mg/l CN
11 mm Round
LCK 316 K316CT.FXD
0.01 - 0.80 mg/l DE
50 mm Rectangular
LCW 017 W017P50.FXD
0.1 - 2.0 mg/l
DE
10 mm Rectangular
LCW 017 W017P10.FXD
0.01 - 1.0 mg/l
mg/l
HCHO
50 mm Rectangular
LCK 325 K325P50.FXD
0.5 - 10.0 mg/l
mg/l
HCHO
11 mm Round
LCK 325 K325CT.FXD
Hydrazine
0.01 - 2.0 mg/l
mg/l N2H4
10 mm Rectangular
LCW 025 W025P10.FXD
Iron
0.01 - 1.0 mg/l
mg/l Fe
11 mm Round
LCK 521 K521CT.FXD
Copper
Cyanide
Detergents, Anionic
Formaldehyde
Spectrophotometer User Guide
98
Analyte
Manganese
Nickel
Nitrogen, Ammonia
Nitrogen, Nitrate
Nitrogen, Nitrite
Units
Cell
Program
0.2 - 6.0 mg/l
mg/l Fe
11 mm Round
LCK 321 K321CT.FXD
0.2 - 6.0 mg/l
mg/l Fe(II)
11 mm Round
LCK 320 K320CT.FXD
0.02 - 1.0 mg/l
mg/l Mn
50 mm Rectangular
LCW 032 W032P50.FXD
0.2 - 5.0 mg/l
mg/l Mn
10 mm Rectangular
LCW 032 W032P10.FXD
0.05 - 1.0 mg/l
mg/l Ni
50 mm Rectangular
LCK 537 K537P50.FXD
0.1 - 6.0 mg/l
mg/l Ni
11 mm Round
LCK 337 K337CT.FXD
0.02 - 2.50 mg/l NH4
0.015 - 2.0 mg/l NH4-N
11 mm Round
LCK 304 K304CT.FXD
1.3 - 15.0 mg/l
1 - 12 mg/l
NH4
NH4-N
11 mm Round
LCK 305 K305CT.FXD
2.5 - 60.0 mg/l
2 - 47 mg/l
NH4
NH4-N
11 mm Round
LCK 303 K303CT.FXD
60 - 167 mg/l
47 - 130 mg/l
NH4
NH4-N
11 mm Round
LCK 302 K302CT.FXD
1 - 60 mg/l
NO3
0.23 - 13.50 mg/l NO3-N
11 mm Round
LCK 339 K339CT.FXD
22 - 155 mg/l
5 - 35 mg/l
NO3
NO3-N
11 mm Round
LCK 340 K340CT.FXD
0.05 - 2.0 mg/l NO2
0.015 - 0.6 mg/l NO2-N
11 mm Round
LCK 341 K341CT.FXD
0.005 - 0.100
NO2
mg/l
0.002 - NO2-N
0.030 mg/l
50 mm Rectangular
LCK 341 K341P50.FXD
2 - 20 mg/l
NO2
NO2-N
11 mm Round
LCK 342 K342CT.FXD
1 – 10 mg/l
mg/l TKN
11 mm Round
LCW909 W909CT.FXD
10 – 200 mg/l
mg/l TKN
11 mm Round
LCK 345 K345CT.FXD
11 mm Round
LCW907 W907CT.FXD
0.6 - 6.0 mg/l
Nitrogen, Total Kjeldahl
AquaMate File Name
Range
200 – 2000 mg/l mg/l TKN
Phenols
0.05 - 5.0 mg/l
Phenol
Organic Complexing Agents
3 – 20 mg/l NTA NTA
Spectrophotometer User Guide
99
AquaMate File Name
Analyte
Range
Units
Cell
Program
Orthophosphate
5 - 90 mg/l
1.6 - 30.0 mg/l
3.7 - 70.0 mg/l
PO4
PO4-P
P2O5
11 mm Round
LCK 049 K049CT.FXD
Phosphorus, Total
0.01 – 0.50 mg/l PO4-P
0.03 – 1.50 mg/l PO4
0.02 – 1.20 mg/l P2O5
50 mm Rectangular
LCK349
0.05 - 1.50 mg/l PO4-P
0.15 - 4.50 mg/l PO4
0.15 - 3.50 mg/l P2O5
11 mm Round
LCK 349 K349CT.FXD
0.5 - 5.0 mg/l
1.5 - 15.0 mg/l
1.2 - 11.5 mg/l
PO4-P
PO4
P2O5
11 mm Round
LCK 348 K348CT.FXD
2 - 20 mg/l
6 - 60 mg/l
4.5 - 45.0 mg/l
PO4-P
PO4
P2O5
11 mm Round
LCK 350 K350CT.FXD
Potassium
8 - 50 mg/l
K
11 mm Round
LCK 328 K328CT.FXD
Silicic Acid
0.01 - 0.80 mg/l SiO2
0.005 - 0.40 mg/l Si
50 mm Rectangular
LCW 028 W028P50.FXD
K349P50.FXD
0.8 – 20 mg/l
0.4 – 10 mg/l
20 – 100 mg/l
10 – 50 mg/l
SiO2
Si
SiO2
Si
11 mm Round
W028CT.FXD
Silver
5 - 400 mg/l
400–2500 mg/l
Ag
11 mm Round
LCK 355 K355CT.FXD
Sulphate
40 - 150 mg/l
SO4
11 mm Round
LCK 153 K153CT.FXD
Sulphide
0.1 - 2.0 mg/l
S2-
10 mm Rectangular
LCW 053 W053P10.FXD
Sulphite
0.1 - 5.0 mg/l
SO3
10 mm Rectangular
LCW 054 W054P10.FXD
Surfactants Determination
0.2 - 2.0 mg/l
11 mm Round
LCK 332 K332CT.FXD
0.5 - 25.0 mg/l
50 mm Rectangular
LCW 018 W018P50.FXD
0.02 – 0.80 mg/l Zn
11 mm Round
LCK360
Zinc
100
Spectrophotometer User Guide
K360CT.FXD
CHEMetrics
Analyte
Method
Range
Program AquaMate File Name
Ammonia
Nessler
0 - 7mg/l
1503
C1503.FXD
(Nitrogen)
Nessler
0 - 14mg/l
1523
C1523.FXD
Bromine
DDPD
0 - 9 mg/l
1603
C1603.FXD
Chlorine
DDPD
0 - 4mg/l
2503
C2503.FXD
DPD
0 - 6mg/l
2513
C2513.FXD
Chlorine Dioxide
DPD
0 - 11mg/l
2703
C2703.FXD
Chromate
Diphenylcarbazide
0 - 3.5mg/l
2803
C2803.FXD
Diphenylcarbazide
0 - 7mg/l
2823
C2823.FXD
Bathocuproine
0 - 7mg/l
3503
C3503.FXD
Bathocuproine
0 - 14mg/l
3523
C3523.FXD
Cyanide
Isonicotinic barbituric acid
0 - 0.4mg/l
3803
C3803.FXD
DEHA
PDTS
0 - 2mg/l
3903
C3903.FXD
Formaldehyde
Purpald
0 - 8mg/l
4203
C4203.FXD
Glycol
Purpald
0 - 10mg/l
4403
C4403.FXD
Hydrazine
PDMAB
0 - 0.7mg/l
5003
C5003.FXD
Iron
Phenanthroline
0 - 6mg/l
6003
C6003.FXD
PDTS
0 - 2.5mg/l
6023
C6023.FXD
Phenanthroline
0 -12mg/l
6013
C6013.FXD
Molybdate
Catechol
0 - 25mg/l
6703
C6703.FXD
Nitrate
Cd Reduction/Chromotrophic Acid
0 - 1.5mg/l
6903
C6903.FXD
Cd Reduction/Chromotrophic Acid
0 - 3mg/l
6923
C6923.FXD
Cd Reduction/Chromotrophic Acid
0 - 60mg/l
6933
C6933.FXD
Nitrite
Azo dye
0 - 0.8mg/l
7003
C7003.FXD
COD
Reactor Digestion
0 - 150mg/l
7350
C7350.FXD
Oxygen Demand,
Reactor Digestion
0 - 1500mg/l
7360
C7360.FXD
Copper
Spectrophotometer User Guide 101
Analyte
Method
Range
Program AquaMate File Name
Chemical
Reactor Digestion
0-15000mg/l
7370
C7370.FXD
Oxygen
Indigo carmine
0 - 2mg/l
7503
C7503.FXD
Indigo carmine
0 - 15mg/l
7513
C7513.FXD
Rhodazine D
0 - 0.8mg/l
7553
C7553.FXD
Ozone
DDPD
0 - 2mg/l
7403
C7403.FXD
Peracetic Acid
DDPD
0 - 4mg/l
7903
C7903.FXD
Peroxide
DDPD
0 - 2mg/l
5503
C5503.FXD
DDPD
0 - 4mg/l
5543
C5543.FXD
4-Aminoantipyrine
0 - 8mg/l
8003
C8003.FXD
4-Aminoantipyrine
0 - 16mg/l
8023
C8023.FXD
Vanadomolybdophosphoric Acid
0 - 40mg/l
8503
C8503.FXD
Stannous Chloride
0 - 5mg/l
8513
C8513.FXD
Silica
Heteropoly Blue
0 - 4mg/l
9003
C9003.FXD
Sulphide
Methylene Blue
0 - 1.5mg/l
9503
C9503.FXD
Methylene Blue
0 - 3mg/l
9523
C9523.FXD
Zincon
0 - 3.0mg/l
9903
C9903.FXD
Zincon
0 - 6.0mg/l
9923
C9923.FXD
Phenols
Phosphate
Zinc
102
Spectrophotometer User Guide
Library
The Local Control Software stores methods and data files in a library. The
term “library” refers to an area of instrument memory called the
“instrument library” and any USB memory device that is formatted as a
library.
You can save files to both types of libraries from the method or results
screen of any Local Control Software application.
Instrument Library screen
To display the instrument library screen, select Library on the Home screen
and press Enter. The instrument displays the file name, file type and
description of each library file. Here are some examples:
* LIBRARY *
TYPE
TEST NAME
FILENAME
M QUANT
M FIXED
D QUANT
D FIXED
M FIXED
UV123
UV146
UV146
UV146
IX2
AB123B
DE146G
TEST
TEST2
THRIB
76% LIBRARY SPACE REMAINING
HIGHLIGHT A FILE AND PRESS ENTER
SMART
PRINT
FORMAT
COPY
ALL
START
DIR
LIBRARY
.QNT
.FXD
.QNT
.FXD
.FXD
VIEW
USB MEM
Instrument library screen for all instruments except the BioMate 6
Spectrophotometer User Guide 103
* LIBRARY *
TYPE
TEST NAME
FILENAME
D SCAN
M BIO
M BIO
M BIO
OLIGOS (CALC) 1A
BRADFORD-MICRO02A
BCA-MICRO2A
TEST
01
B2
BC2
76% LIBRARY SPACE REMAINING
HIGHLIGHT A FILE AND PRESS ENTER
ALL
PRINT
FORMAT
COPY
ALL
FILES
DIR
LIBRARY
.SCN
.NAM
.PRM
.PRM
VIEW
USB MEM
Instrument library screen for the BioMate 6
Use the arrow keys to display the next or previous page.
Note
There may be a short delay while the instrument loads the next section of
the directory.
Parameter
Function
Type
Describes the file and the type of information it contains. Here
are some examples:
M Scan
D Scan
M Fixed
D Fixed
M Quant
D Quant
M Rate
D Rate
M MCA
D MCA
M Bio
D Bio
Scan method
Spectrum, data and method
Fixed wavelength method
Fixed wavelength results and method
Quant method, including calibration
Quant results, including method and calibration
Rate method
Rate graphics, data and method
MCA method
MCA results
BioMate test
BioMate test and results
Type is assigned automatically depending on the application
used to create the file and the file’s contents.
104
Spectrophotometer User Guide
Test Name
Shows the descriptive name that was entered when the file was
saved (see Test Name in the method screen for any Local
Control Software application).
Filename
Shows the DOS-compatible file name used by the instrument or
computer. You can edit or rename the file. See Working with
Files Stored in the Instrument Library for details.
Function key
Description
View USB MEM
Displays the directory for the USB memory device that is
currently in the drive.
Copy All
Copies all files from the instrument library to the installed USB
memory device.
Note: Before you select this option, install a compatible USB
memory device that has available space.
Format Library
DELETES all files from the instrument library.
Print Dir
Prints the directory for the instrument library.
Smart Start
Available on all instruments except the BioMate 6.
Select a file to be displayed in the start-up menu and press
Enter. The start-up menu will appear when the instrument is
turned on (instead of the default Home screen).
Press Home to see the new start-up screen.
From the start-up screen, press General Tests to display the
default Home screen.
All Files /Results
Only
Available on BioMate 6 models only. Toggles between two
display formats: methods and results combined, or results only.
Working with files stored in the instrument library
To perform an operation on a library file, select the file and press Enter.
The Instrument Library pop-up menu is displayed:
DATA1
.FXD
LOAD
RENAME
SAVE TO USB MEM
DELETE
Select an option and press Enter.
Menu Option
Function
Load
Loads the selected method or displays the selected results.
Rename
Displays the Save/Rename screen where file name can be
changed.
Save to USB
MEM
Copies the selected file to the installed USB memory device.
Delete
Removes the selected file from the instrument library.
Spectrophotometer User Guide 105
USB Memory Device Library screen
To display the library screen for the installed USB Memory Device, insert
the device, display the Instrument Library screen and select View USB
Mem. The instrument displays the file name, file type and description of
each library file.
Use the arrow keys to display the next or previous page.
Note
There may be a short delay while the instrument loads the next section of
the directory.
Parameter
Function
Type
Describes the file and the type of information it contains. See
Instrument Library screen for examples.
Type is assigned automatically depending on the application
used to create the file and the file’s contents.
106
Spectrophotometer User Guide
Test Name
Shows the descriptive name that was entered when the file was
saved (see Test Name in the method screen for any Local
Control Software application).
Filename
Shows the DOS-compatible file name used by the instrument or
computer. You can edit or rename the file. See Working with
Files Stored on a USB Memory Device for details.
Function key
Description
View Library
Displays the directory for the instrument library.
Read USB Mem
Refreshes the directory for the installed USB memory device.
Print Dir
Prints the directory for the installed USB memory device.
Copy All
Copies all files from the installed USB memory device to the
instrument library.
Working with files stored on a USB Memory device
To perform an operation on a library file, select the file and press Enter.
The USB Library pop-up menu is displayed:
TESTFILE.FXD
LOAD
RENAME
SAVE TO LIBRARY
DELETE
Select an option and press Enter.
Parameter
Function
Load
Loads the selected method or displays the selected results.
Rename
Displays the Save/Rename screen so you can save the file with a
different file name and description.
Save to Library
Copies the selected file to the instrument library.
Delete
Removes the selected file from the installed USB memory device.
Spectrophotometer User Guide 107
UVcalc
Quantitative analytical procedures are built around two fundamental
principles, measurement of the parameter, and subsequent calculations
based on these measurements.
In UV-Visible spectrophotometry and many other ‘mature’ analytical
techniques, the science associated with the measurement of the parameter is
well developed. There are fully-validated ‘test kits’ available from the
leading chemical suppliers in the key areas of bio-chemical and
environmental/water chemistry. In addition, most laboratories also have
their own fully-developed internal procedures.
With defined procedures, many ‘standard methods’ document the final
calculation in the form of an algebraic formula. UVcalc allows these
formulae to be entered into the software method, together with the control
limits.
UVcalc provides automatic calculation of results from measurements using
user-defined equations. The measurement is obtained from the
spectrometer in the form of a reading at a specific wavelength in Scan
methods and individual results in Fixed and Quant methods.
Spectrophotometer User Guide 108
Specification
Up to 4 different equations may be applied to each measurement.
The formula editor supports +, -, *, /, and bracketing.
Allowed operands include measurements, constants (entered via the
numeric keypad), fixed & variable factors (input by the user at run-time)
and UVcalc results from preceding equations.
Each formula may have up to 20 characters.
Each formula supports up to 9 terms including measurements, constants,
variable factors and results.
Equations are automatically saved with the method. If you save sample
results that are produced with a method that contains calculations, the
calculations are also saved with the sample data.
Equations, results, units and pass/fail results are included on the hard copy
output.
Operation
When the UVcalc software is installed, the instrument adds the UVcalc
option to the Scan, Fixed and Quant screens. When no equations are
programmed, the UVcalc field is set to 0.
Select UVcalc and press Enter to display a list of up to four UVcalc
equations (empty when first installed).
* UVCALC *
EQUATION
EQUATION
EQUATION
EQUATION
CANCEL
1
2
3
4
:
:
:
:
ACCEPT
Spectrophotometer User Guide 109
To create or edit an equation, select an equation in the list and press Enter.
The equations parameters screen is displayed in the following format:
Formula
Title
Units
Test Result: No
Upper Limit: 0.000
Lower Limit: 0.000
There will also be lines specific to the particular application (Scan, Fixed or
Quant).
Option
Function
Formula
Defines the terms and operands in the formula.
Title
Specifies a name for the formula. Use the Text Entry screen to
enter a name and press Accept.
Units
Gives the units for the equation. Use the Text Entry screen to
enter a name and press Accept.
Test Result
Toggles between Yes and No.
Upper/Lower
Limit
Define the allowable limits for the test.
Function key
Description
Accept
Stores the entered settings.
Cancel
Displays the Equations screen without storing your entries.
Defining a formula
To enter a formula, select Formula and press Enter. The instrument
displays a simulated keyboard with the following symbols:
λ or M
F
R
(
)
+
-
*
/
Space
Each symbol represents an available formula term or operand (see the table
below for definitions). Use the symbols to build a formula. To add a term
or operand to the formula, select the corresponding symbol and press
Enter.
110
Spectrophotometer User Guide
Option
Function
λ or M
λ (for UVcalc from a Scan Method)- Displays a pop-up entry box to
define the wavelength for the measurement.
M (for UVcalc from a Fixed or Quant method)- Displays a pop up
menu with the following options:
Once Only (Constant) – Measures this value the first time and then
uses that value for subsequent calculations.
Measure Each Run – Measures this value each time.
F
Defines a factor. Each factor can be fixed or variable (can be
entered by the user at run time).
R
UVcalc result from preceding equations.
( and )
Can be used to group terms and operands.
+, -, *, /
Adds the corresponding operand (add, subtract, multiply, divide) to
the formula.
Space
Adds a space to the formula.
Function key
Description
Å
Clears the last character in the formula.
Switch Fields
Toggles the cursor between the formula and the simulated
keyboard.
Accept
Stores the entered settings.
Cancel
Displays the Equations screen without storing your entries.
To clear the entire formula, press C.
To store the formula as displayed, press Accept. The Equations screen is
displayed with the new formula listed in the next available line.
Setting up a Scan calculation
Up to 9 different measurements may be specified for each Scan UVcalc
equation. These are denoted by 1……9. The wavelengths at which these
measurements are made can be specified in one of 2 ways. They can be
entered numerically before the scan, or they can be entered after the scan
using a peak picking process. This appears as an extra parameter on the
equation parameter screen (Use Tracking). The selected wavelengths will
then be fed into the method so that the subsequent final result will be
calculated automatically.
Spectrophotometer User Guide 111
If a factor is used only once in a calculation, the instrument prompts the
operator to input the factor before the first scan.
Note
If you subsequently move from the Results/Graph screens back to the main
menu, the one-off factors will be reset and must be re-entered before the
next run.
This example shows how to set up the following Scan calculation:
λ1/λ2 * F
1. Display the Scan method parameters.
For this example, set Start to 400 nm and Stop to 600 nm.
2. Select UVcalc and press Enter
3. Select Equation 1 and press Enter.
4. Select Formula and press Enter.
5. Select λ and press Enter.
6. Enter 450 for the first wavelength and press Enter.
7. Select / and press Enter.
8. Select λ and press Enter.
9. Enter 500 and press Enter.
10. Select * and press Enter.
11. Select F and press Enter.
A pop-up menu is displayed with the following options:
•
•
Fixed Factor
Variable Factor
12. Select Variable Factor and press Enter.
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Spectrophotometer User Guide
13. Enter a suitable ID and press Accept.
The formula displayed at the top of the screen should look like this:
FORMULA : λ1/λ2*F1
14. Press Accept to complete the formula.
15. Select Title, enter a descriptive name to be displayed in the
UVCALC Equations screen and press Accept.
16. Select Units, enter appropriate units for the equation and press
Accept.
17. Press Accept again to complete the equation.
The new equation is displayed in the Equations screen:
MY CALC : λ1/λ2*F1
18. Press Accept again to return to the Scan parameters screen.
19. Insert the sample and press Run.
20. Enter a factor at the prompt and press Accept.
The instrument scans the sample and displays the results of the calculation.
Setting up a Fixed calculation
Up to 9 different measured results may be specified for each UVcalc
equation. These measurements will comprise a combination of up to 9
different one-off measurements (measured at the start of the run only) and
one measurement which will be re-measured each time Run is pressed.
This example shows how to set up the following Fixed calculation:
M1 *50.0
1. Display the Fixed method parameters, select UVcalc and press
Enter.
2. Select Equation 1 and press Enter.
Spectrophotometer User Guide 113
3. Select Formula and press Enter.
4. Select M and press Enter.
The instrument displays a pop-up menu with the following options:
•
•
Once only – constant
Measure each RUN
5. Select Measure each RUN and press Enter.
6. Select * and press Enter.
7. Enter 50 and press Accept.
The formula displayed at the top of the screen should look like this:
FORMULA : M1 * 50
8. Select Title, enter a descriptive name to be displayed in the
UVCALC Equations screen and press Accept.
9. Select Units, enter appropriate units for the equation and press
Accept.
10. Press Accept to complete the equation.
The new equation is displayed in the Equations screen:
MY CALC : M1 * 50
11. Press Accept again to return to the Fixed parameters screen.
12. Insert the sample and press Run.
The instrument measures the sample and displays the actual absorbance
value and the result of the calculation.
Setting up a Quant calculation
You can specify up to 9 different measurements. These measurements may
be a standard (S1…S6) (measured as part of the normal calibration process)
or a sample (X).
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Spectrophotometer User Guide
Note
One-off factors will be cleared if you select Clear Results from the results
screen or if you return to the main menu.
Modifying an equation by adding parameters
This example shows how to add parameters to the end of an equation (e.g.,
adds a weight correction to M1*50).
M1*50.0*(F1/F2)
where:
F1 = Nominal weight (Fixed Factor)
F2 = Actual weight (Variable Factor)
1. Display the Fixed method parameters, select UVcalc and press
Enter.
2. Select Equation 1 and press Enter.
The equation prepared above is displayed.
M1 *50.0
3. Select Formula and press Enter.
The cursor moves to the end of the existing formula.
4. Select * and press Enter.
5. Select ( and press Enter.
6. Select F and press Enter.
7. Select Fixed Factor and press Enter.
8. Enter a suitable ID for the factor and press Accept.
9. Select / and press Enter.
10. Select F and press Enter.
11. Select Variable Factor and press Enter.
12. Enter a suitable ID for the factor and press Accept.
13. Select ) and press Enter.
Spectrophotometer User Guide 115
14. Press Accept to display the main UVcalc screen.
The formula displayed at the top of the screen should look like this:
FORMULA : M1 * 50 * (F1/F2)
15. Press Accept twice to display the Fixed parameters screen.
16. Insert the sample and press Run.
17. Enter a factor at the prompt and press Accept.
Modifying an equation by adding constants
This example shows how to add constants to an equation (e.g., adds a
second constant to M1*50).
(M2-M1)*50.0
where:
M1 = becomes a once only constant
M2 = measure with each run
1. Display the Fixed method parameters, select UVcalc and press
Enter.
2. Select Equation 1 and press Enter.
The equation prepared above is displayed.
M1 *50.0
3. Select Formula and press Enter.
The cursor moves to the end of the existing formula.
4. Press Switch Fields to select the formula, move the cursor to M1
and press Enter.
5. Change the selection to Once Only – Constant and press Enter.
6. Press Switch Fields to select the simulated keyboard.
7. Select ( and press Enter.
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Spectrophotometer User Guide
8. Select M, press Enter, select Measure with each RUN and press
Enter.
9. Select “-“ (minus) and press Enter.
10. Press Switch Fields and move the cursor to the *.
11. Press Switch Fields again, select ) and press Enter.
12. Press Accept.
The formula displayed at the top of the screen should look like this:
FORMULA : (M2-M1) * 50
13. Make appropriate entries for Title and Units.
14. Press Accept twice to display the Fixed parameters screen.
UVcalc Error Messages
The following error messages may occur if you make a mistake in entering
an equation or in setting up the system.
Error Message
Problem
ONLY 1 FACTOR MAY BE ENTERED WITH SAMPLE
Formula has two or more factors for each sample.
THIS FORMULA HAS TOO MANY CONSTANTS
Formula has more than 9 numbers.
FORMULA CONTAINS AN INVALID NUMBER
Formula has an invalid input.
BRACES DO NOT MATCH IN FORMULA
Formula has too many brackets at one end.
ALL BINARY OPERATIONS REQUIRE TWO
OPERANDS
Formula has an incomplete arithmetical operation
(e.g., 3-+4).
INVALID COMBINATION OF OPERANDS
You have created a formula with missing user(s) (e.g. F1(M1)).
BRACE MISSING?- UNMATCHED CLOSE BRACE
Closing bracket appears before or without an open bracket.
THE FORMULA CANNOT START WITH THIS TOKEN
Quant mode formula has an invalid initial token; i.e., a user rather than an
operand.
FORMULA CONTAINS OUT OF RANGE STANDARD
A specified standard is no longer in the calibration.
ONLY ONE MULTIPLE MEASUREMENT IS ALLOWED
Fixed mode formula has an invalid initial token.
FORMULA CONTAINS INVALID RESULT TOKEN
The result from an earlier calculation is no longer being produced.
Spectrophotometer User Guide 117
Error Message
Problem
UVCALC :INVALID CELL CHANGER MODE
An invalid Cell Changer mode has been selected. Check settings.
FORMULA CONTAINS OUT OF RANGE
WAVELENGTH
You have selected wavelengths outside the range set for the scan.
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Spectrophotometer User Guide
Setup
Use the Setup function key on the Home screen to access general
instrument parameters. Select a parameter in the list and choose Enter.
To change a parameter setting, select the parameter and press Enter. See
Parameter Entry for more information.
Setup screen
* SETUP *
CLOCK
PRINTER
ENVIRONMENT
WAVELENGTH
INITIALIZE
WHITE LIGHT
CVC
RECORDER
ACCESSORIES
LAMPS
Parameter
Function
Clock
Sets the internal time and date.
Printer
Selects the printer type.
Environment
Sets language, date format, file type, autosave, autoprint, and
user log-on options.
Wavelength
Used for wavelength recalibration.
Initialize
Defines instrument initialization and default baseline.
White Light
Sets zero order for alignment of sample holders.
CVC
Loads CVC calibration data.
Recorder
Allows analog data output.
Spectrophotometer User Guide 119
Function key
Description
Accessories
Displays a pop-up menu to access parameters for accessories
such as the Cell Changer or sipper.
Lamps
Shows lamp status and energy levels, turns lamps on and off,
and resets lamp hours. See Lamps screen for more information.
Clock screen
* CLOCK *
* TIME *
HOURS
MINS
:
:
16
32
* DATE *
DAY
MONTH
YEAR
:
:
:
25
12
08
SETUP
PAGE
CANCEL
ACCEPT
Select this option to set the instrument’s internal time and date. To reset a
parameter, select the parameter and press Enter to display a pop-up entry
box. Type the new value and press Enter. When you are finished setting
parameters, press Accept to save the new settings or Cancel to exit without
saving. Press Setup Page to return to the Setup screen.
Printer menu options
Select this option to set up a printer. The Printer Type is displayed and is
set to the currently selected printer.
To change the selected printer, press Enter. A pop-up menu is displayed
with a list of supported printers. Select a printer and press Enter.
PRINTER
HP LASERJET
HP INKJET
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Spectrophotometer User Guide
Note
Menu Option
Description
HP LaserJet
HP LaserJet Series with PCL 3 only.
HP InkJet
HP InkJet Series with PCL 3 only.
Function key
Description
Setup Page
Displays the Setup screen.
Printers designed to work only in a Windows® environment may not be
compatible with Local Control software.
Before you attempt to print at any point during operation of the
instrument, make sure the printer is ready to print (i.e., power is on,
printer is on-line, paper is loaded). Failure to do so will result in an error
condition. Press ESC to clear the error message. Then correct the problem
with the printer and try again.
Environment screen
Use this screen to turn the beeper on and off and set the date format, the
language used for the software and the default file type used to save results.
You can also enable or disable automatic calibration verification, LIMS
(Laboratory Information Management System) output and the UVcalc
application. These parameters are described in more detail below.
* ENVIRONMENT *
LANGUAGE
: ENGLISH
SOUND
: OFF
DATE FORMAT
: dd/MM/yy
AUTOMATIC CAL. VAL
: OFF
DEFAULT FILE TYPE
: NORMAL
LIMS SUPPORT
: OFF
USE SAMPLE IDS
: OFF
AUTOSAVE RESULTS
: OFF
AUTOPRINT RESULTS
: OFF
USER LOG-ON
: 0FF
HISTORY FILE
: OFF
SETUP
PAGE
HISTORY
FILE
Spectrophotometer User Guide 121
Function key
Description
History File
Appears only when the History File option is enabled.
Change Users
Appears only when User Log-On is enabled and is available
only to the system administrator.
UVcalc Off
Appears only when the UVcalc application is installed. Disables
UVcalc and re-enables functions that UVcalc disables.
Setup Page
Displays the Setup screen.
Language
Sets the language used on the display. A pop-up menu lists the available
languages. Select an option and press Enter. The language used
immediately changes to the one selected.
Sound
Toggles the audio warning on and off. When Sound is set to Off, error
conditions are indicated by on-screen messages only.
Date Format
Sets the date format. A pop-up menu lists the available formats, depending
on whether the day or month is entered first and the number of characters
entered for the year. Select an option and press Enter.
Automatic Cal. Val.
Toggles the power-up performance verification option on and off. The
optional Calibration Verification Carousel (CVC) must be installed to use
this option.
When this option is On and the CVC is installed, the instrument
automatically waits on start-up for the warm-up period (60 minutes) and
then performs the Wavelength, Absorbance and UV Absorbance
calibration tests (see CVC section). Pressing ESC cancels the calibration.
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Spectrophotometer User Guide
Default File Type
Selects the default file type that is displayed when you use the Save/Rename
function to save methods and data.
DEFAULT FILE TYPE
NORMAL
CSV
JCAMP-DX
Menu Option
Function
Normal
The native file type used by the Local Control software.
Note: This is the only option available when saving a
test file.
CSV
Comma Separated Variable.
JCAMP-DX
JCAMP data exchange format.
LIMS Support
Toggles the LIMS (Laboratory Information Management System) output
option on and off.
When this option is On, after each measurement the software
automatically exports results, methods and sample IDs (when selected) to
the central LIMS computer via the RS232 port.
Note
Make sure the LIMS interface is connected before you active the LIMS
Support option in the software.
Use Sample IDs
Sets up automatic sample identification.
DEFAULT FILE TYPE
OFF
SEEDED
PROMPT USER
Spectrophotometer User Guide 123
Menu Option
Function
Off
The system does not attach an identity to the sample.
Seeded
Stores a user-defined identification with each sample. The
sample ID is displayed and any printed results. When LIMS
Support is enabled, the sample ID is also exported with the
sample results and the method used.
When Use Sample ID is set to Seeded, the following options
appear in the Environment screen:
Sample ID – Displays the Text Entry screen. Enter a base name
for the samples (up to 11 characters) and press Accept.
Sample ID Seeded – Displays a pop-up entry box. Enter the
number you want to assign to the first sample and press Enter
(e.g., enter 0 to identify the first sample as 1). The number is
incremented automatically before each sample.
Prompt User
Allows the operator to identify each sample at run time. Before
each run, the Text Entry screen is displayed and the user is
prompted to enter a name for the sample.
Note
When the Cell Changer is used in Auto mode, the Sample ID is
incremented automatically without stopping for ID confirmation between
samples.
Note
The Prompt User option does not work when the Sipper is operating in the
Sip & Run or AutoSampler mode.
AutoSave Result
Toggles the AutoSave option on and off.
Option
Function
On
Sample results are saved automatically after each run.
When AutoSave Result is set to On, the following options are displayed
in the Environment screen.
Filename – Displays the Text Entry screen. Enter a base filename (up to
5 characters) and press Accept.
File Number – Displays a pop-up entry box. Enter the number (0 to 999)
you want to assign to the first sample file and press Enter. The number is
appended to the filename and incremented automatically after each run.
Off
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Spectrophotometer User Guide
Sample results are not saved automatically. Use Save Data, where
applicable, to save a measurement.
AutoPrint Results
Toggles the AutoPrint option on and off.
Note
Option
Function
On
Prints sample results automatically after each run.
Off
Does not print results automatically.
Before you attempt to print at any point during operation of the
instrument, make sure the printer is ready to print (i.e., power is on,
printer is on-line, paper is loaded). Failure to do so will result in an error
condition. Press ESC to clear the error message. Then correct the problem
with the printer and try again.
User Log-On
Toggles the User Log-On option on and off. When User Log-On is set to
On, the system administrator controls what each user can do with the
instrument.
Note
We recommend that you activate User Log-On if you have a multi-user
environment.
Option
Function
On
Displays the Text Entry screen. Enter the correct password to set
up administrative access to the software and press Accept. The
default password is ADMIN (password is case sensitive).
Note: If you enter the wrong password, an error message appears
and User Log-On remains set to Off.
When User Log-On is enabled, each time the instrument is
powered up the system prompts the user to log on by entering the
correct user name and password. This allows access only to
functions that are enabled for each user by the system
administrator. At the close of a session, the user logs off by
pressing Log Off on the Home screen and choosing whether to
Proceed or Stop. When Proceed is selected, the system waits for
the next user to enter his or her name.
Off (default)
Every user can access all the functions available for the
instrument.
Spectrophotometer User Guide 125
Function key
Description
Change Users
Available only when User Log-On is set to On. Allows the system
administrator to set up a user name and password and define
access for each user.
Change Users screen
* CURRENT USERS *
NAME
PASSWORD
ADMIN
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
ADMIN
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
−−−−−−−−−−
E = EDIT METHODS
C = CALIBRATIONS
F = DELETE FILES
I = INITIALISATIONS
CANCEL
ACCEPT
PRIVILEGES
E C F I H L B
√ √ √ √ √ √ √
− − − − − − −
− − − − − − −
− − − − − − −
− − − − − − −
− − − − − − −
− − − − − − −
− − − − − − −
− − − − − − −
− − − − − − −
H = HISTORY FILE
L = RESET LIFETIMES
B = DEFAULT BASELINE
USERS
11 - 20
The system administrator can use this screen to add up to 20 names to the
user list. Each user must have an associated password. Use the Privileges
columns to enable each user access to the available functions. Each function
has an assigned letter ID; the available functions are listed at the bottom of
the screen.
Note
We strongly recommend that you enter a new user name and password for
the system administrator as soon as you activate User Log-On.
Only the Administrator (listed as ADMIN in the user list) can change
passwords, edit the Current Users Screen and reset User Log-On to Off.
Notice
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Spectrophotometer User Guide
Resetting User Log-On to Off clears the list of users and resets the default
User Name and Password to ADMIN.
History File
Toggles the History File option on and off. When this option is On, the
system automatically logs changes to the instrument in the history file. The
following changes are logged with the date, time and user:
•
•
•
•
•
Changes to default baselines
Wavelength calibrations
Sipper calibrations
CVC tests
Maintenance operations (recorded by our service engineers).
When History File is set to On, the History File function key appears on
the Environment screen (unless User Log-On is enabled and the system
administrator has not granted History File access to the current user).
History file menu options
The History File function key displays the History File pop-up menu.
HISTORY FILE
SAVE HISTORY ON USB MEMORY
CLEAR HISTORY
PRINT HISTORY
Item
Function
Save History on
USB Memory
Prompts for a file name and saves the instrument history in CSV
format, which may be read by a compatible spreadsheet or text
editor.
Clear History
Clears the instrument history to make room for more entries.
The History File contains a maximum of 400 entries. When the
number of entries reaches 390, a warning message appears.
The Administrator or a user with the History File privilege
should save and/or print the existing history file and then clear
the file’s contents.
Print History
Prints the instrument history using the selected printer.
Note: Make sure the printer is connected and turned on before
you select this option.
Spectrophotometer User Guide 127
Wavelength Calibration screen
This option is not available for the AquaMate Plus UV-Vis model.
Select this option to optimize the instrument’s wavelength calibration.
* WAVELENGTH CALIBRATION *
CALIBRATION
: USING D2 LAMP
SETUP
PAGE
CALIBRATE
Calibration measures the deuterium lamp emission line at 656.1 nm and
adjusts the calibration accordingly.
Calibrate only when the instrument no longer achieves its quoted
wavelength accuracy specification.
Notice
Do not attempt to recalibrate the instrument unless you are absolutely sure
you need to do so. If you have questions, contact our technical support.
Notice
Clear the sample and reference beams before you recalibrate.
The calibration takes at least 10 minutes. Follow these steps:
1. Make sure the deuterium lamp is On.
2. Wait until the instrument is fully warmed up.
3. Clear the sample and reference beams.
4. From the Wavelength Calibration screen, press Calibrate.
5. When calibration is complete, turn off the instrument.
6. Turn on the instrument.
7. Turn on the deuterium lamp and wait for it to warm up.
8. Run Default Baseline (see Optical Initialization screen).
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Spectrophotometer User Guide
Optical Initialization screen
Use this screen to reset the instrument and define its initialization and
default baseline. These procedures ensure the optimum performance of the
spectrophotometer.
* INITIALIZE*
INITIALIZATION TYPE
: OPTICS
INITIALIZE WITH D2
: ON
SETUP
PAGE
INITIALIZE
Option
Description
Initialization Type
Selects the initialization type:
Optics – During initialization, the instrument performs simple
hardware checks, calculates data tables and measures the dark
current. The filter wheel is then initialized before the instrument
drives to the default wavelength and performs an autozero.
Baseline – During initialization, the instrument re-measures the
default baseline. This process takes about one hour to complete.
Measure the default baseline after you change a source lamp or
perform wavelength calibration and when the instrument is
working at temperatures significantly different from 25 °C.
Note: Before you measure the baseline, make sure both lamps
are on and the spectrophotometer is fully warmed up.
Initialize with D2
Selects whether the instrument will initialize with or without the
deuterium lamp. When this option is On, the instrument
automatically strikes the deuterium lamp during initialization.
Note: This option is unavailable for the AquaMate Plus UV-Vis
model.
Function key
Description
Initialize
Performs the selected initialization type (optics or baseline).
Spectrophotometer User Guide 129
White Light screen
Use this option to align the grating so the zero order diffraction (white
light) passes through the sample compartment. You can see the beam when
you place a white card or similar item in the light path. The visible beam
can be helpful for aligning optical accessories in the sample compartment.
In double beam instruments, the action of the chopper causes the light to
alternate between the sample and reference beams.
To realign the grating, press Initialize.
When alignment is completed, press Stop to return the grating to its
normal position. To display the Setup screen, press Setup Page.
Setup CVC screen
Use this option to access the options available for setting up and running
the Calibration Verification Carousel (CVC). See the CVC section of this
manual for more information.
Lamps screen
The Lamp functions can be accessed from the Setup screen or from the
Home screen, if User Log-on is not in use. The Lamps screen shows the
energy level and status (On, Off or Failed) of the available lamps
(deuterium and/or tungsten halogen). The Lamps screen function keys
differ depending on the instrument model.
TUNGSTEN
HOURS
ENERGY
:
:
:
* LAMPS *
ON
239
10%
D2
HOURS
ENERGY
:
:
:
OFF
55
0%
SWITC
H
D2
D2
ENERG
Y
W
ENERGY
RESET
D2 HRS
RESET
W HRS
For AquaMate Plus, BioMate 6, Omega, Evolution 160
and UV-10 spectrophotometers
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Spectrophotometer User Guide
TUNGSTEN
HOURS
ENERGY
:
:
:
* LAMPS *
ON
239
10%
D2
HOURS
ENERGY
:
:
:
OFF
55
0%
W
ENERGY
RESET
W HRS
For AquaMate Vis spectrophotometer
Parameter
Function
Tungsten
Shows the status of the tungsten halogen lamp (On, Off or
Failed).
Hours
Shows the total hours the tungsten lamp has been used.
Replace the lamp after 2000 hours of use and then reset the
hours to zero (see Reset W Hrs function key).
Energy
D2
Hours
Shows the energy level of the tungsten lamp (50%–100%).
Shows the status of the deuterium lamp (On, Off or Failed).
Shows the total hours the deuterium lamp has been used.
Replace the lamp after 1000 hours of use and then reset the
hours to zero (see Reset W Hrs function key).
Energy
Shows the relative energy level of the deuterium lamp (50%–
100%).
Function key
Description
Reset W HRS
or
Reset D2 Hrs
Resets the hours for the selected lamp (W=Tungsten,
D2=Deterium).
Note: Allow the lamp at least 10 minutes to warm up before
resetting its hours.
A pop-up menu appears with two options (Proceed or Cancel).
Select Proceed and press Enter to reset. The hours change to
zero and the Energy readout shows the energy measured at the
appropriate wavelength.
W Energy
Measures the energy for the selected lamp.
Note: Clear the sample and reference beam and allow the lamp
at least 10 minutes to warm up before measuring its energy.
To exit this screen, press Home.
Spectrophotometer User Guide 131
Cell Changer
The 7-Cell Changer allows you to present up to seven samples for
sequential measurement. The Cell Changer is available as a standard option
on all spectrophotometer models.
This section describes how to install, operate and remove the accessory.
Installing and removing the Cell Changer
Follow these steps carefully each time you remove or install the Cell
Changer carousel.
Installing the Cell Changer
To install the Cell Changer:
1. Remove the cover from the motor drive.
2. Remove the single cell holder or other accessory from the sample
compartment.
3. Install the Cell Changer carousel.
Place the carousel on the motor shaft mounted inside the sample
compartment, taking care to align the tab on the underside of the
carousel with the notch on the motor shaft.
Tab on underside
of carousel
Notch on motor shaft of
Smart base unit
Spectrophotometer User Guide 132
4. Tighten the screw on the top of the carousel by turning the knurled
knob clockwise.
5. From the Cell Prog screen, press Initialize to align the carousel
with the spectrophotometer.
Notice
This step is important to ensure correct operation of the Cell Changer. We
recommend that you initialize the Cell Changer each time you use it.
Removing the Cell Changer
To remove the Cell Changer:
1. Hold the carousel firmly and turn the central screw
counterclockwise until the carousel is released.
2. Lift the carousel off the motor shaft.
3. Replace the cover on the motor drive.
Operating the Cell Changer
When the Cell Changer is installed, a status box is displayed indicating the
presence of the accessory and its current position.
If the status box is displayed, you can use the right/left arrow keys to
manually move the Cell Changer. The software tracks the number of times
the arrow keys are used. For example, press the right arrow key 5 times to
advance the Cell Changer exactly 5 positions.
* PROG *
CELL
CELL POS.
MODE
REF. MODE
LAST CELL
CELL CYCLES
SPEED
:
:
:
:
:
:
1
MANUAL
OFF
7
5
HIGH
INIT
IALIZE
For all instrument models except the BioMate
Spectrophotometer User Guide 133
* PROG *
CELL
CELL POS.
MODE
SPEED
: 1
: ON
: HIGH
INIT
IALIZE
For the BioMate 6
Item
Function
Cell Pos
Shows the current position of the Cell Changer. Use the right/left
arrow keys to move the Cell Changer.
Mode
Selects the Cell Changer run mode.
Manual – Cell changer can be operated manually. Use the right/left
arrow keys to position a cell.
Run&Step – Measures the current cell and automatically moves to
the next cell.
Auto – Cell changer operates automatically (measures each cell in
order). The number of cells measured (maximum of 7) depends on
the value of the Last Cell parameter.
Off – Turns off the Cell Changer. The instrument behaves as if it has
a single cell only.
Note: For the BioMate 6, Mode can only be set to On or Off.
Ref. Mode
Toggles the Reference mode on and off. When On, assigns cell
number 1 as the Reference position and performs a zero or baseline
measurement on that cell. This is true for all Mode settings above.
Note: This parameters is not available for the BioMate 6.
Last Cell
Defines the last cell to be measured in a sequence. Can be set from
1 to 7.
Note: This parameters is not available for the BioMate 6.
Cell Cycles
When Mode is set to Auto, this parameter defines the number of
measurements (cycles) performed on each cell (up to 300). For
example, set Cell Cycles to 4 to measure each cell four times
according to the current method.
Note: This parameters is not available for the BioMate 6.
Speed
134
Spectrophotometer User Guide
Sets the Cell Changer speed of rotation (High, Medium or Low).
Function key
Description
Initialize
Resets the Cell Changer and places cell number 1 in the sample
beam.
Spectrophotometer User Guide 135
SuperSipper
The SuperSipper is an optional accessory that enables samples to be drawn
into a flow cell for automatic measurement. The SuperSipper works with
any kind of flow cell.
After the measurement is complete, the sample may be sent to a waste
receptacle or returned to its original vessel. A continuous pumping mode
can be used to run a rinse solution through the system when required
e.g., between applications.
This section describes how to use Local Control Software to operate the
SuperSipper. For complete installation and operating instructions for this
accessory, see the corresponding manual on the documentation CD.
To operate the SuperSipper, install the accessory as described in the
operating manual. Present the sample to the SuperSipper and press the
switch to draw the required sample volume into the tubing. When the
system beeps, remove the sample to allow the system to draw in the
required air gap. When the measurement is completed, press the switch
again to empty the flow cell. The sample is pumped to a waste receptacle or
the original sample vessel.
When the sipper is connected, a status box appears on the right side of
every method screens. The status box indicates the presence of the
SuperSipper and its status.
Spectrophotometer User Guide 136
Sipper screen
* SIPPER *
SIPPER
MODE
AIR GAP
SAMPLE VOL.
SAMPLE
LOW VOL.
:
:
:
:
:
:
VIEW
CALIB
OFF
SIP
50 cm
1.000 ml
WASTE
OFF
CALIBRATE
Use this screen to set up the SuperSipper for the required analysis. These
parameters are saved with any data produced by the software.
To reach this screen, press Accessories from the Home screen, then select
Sipper and press Enter. To change a parameter setting, select the parameter
and press Enter. See Parameter Entry for more information.
Item
Function
Sipper
Sets the Sipper status (On, Off or Standby).
Mode
Selects the run mode for the SuperSipper.
Sip – Sets the system to fill the flow cell. If Sample is set to Return,
then alternate switch presses will fill and empty the flow cell. In this
mode, instrument operation is completely independent of the
SuperSipper.
Sip&Run – Sets the system to fill the flow cell and automatically
perform a measurement. If Sample is set to Return, then alternate
switch presses will fill and empty the flow cell. The current method
used to produce the result (e.g., if Fixed is current, then the sample
will be measured using the Fixed method as programmed).
Continuous – Sets the system to pump continuously to waste.
Alternate switch presses will start and stop pumping. In this mode,
instrument operation is completely independent of the SuperSipper.
AutoSam – Configures the Sipper to operate with an autosampler.
Air Gap
Enter value between 0 and 500 cm.
Sets the gap between the trailing meniscus of the current sample
and the leading meniscus of the next sample. The gap is measured
to the nearest centimeter.
For best results, set the airgap to ≥ 8 cm from the flow cell.
Spectrophotometer User Guide 137
Item
Function
Sample Vol
Enter a value between 0.2 and 10.000 ml.
Sets the volume of sample to be pumped.
Sample
Selects from Waste or Return.
Waste – After measurement, the sample is pumped through the
flow cell to waste by the act of pumping the next sample.
Return – After measurement, the pump direction is reversed and
the sample is returned to the sample vessel.
Low Vol
Toggles on or off.
Automatically adjusts the pumping time to maintain the correct air
gap for narrow uptake tubing.
Off – Use standard internal diameter (1.1 mm) uptake tube.
On – Use narrow internal diameter (0.8 mm) uptake tube.
Function key
Description
View Calib
Displays the Sipper Calibration screen.
Calibrate
Starts the Sipper calibration procedure.
SuperSipper Calibration
Calibration adjusts the SuperSipper for variations in pump and uptake
tubing and sample viscosities. During calibration, you enter the volume
you want to extract from the sample and the system performs several sips
using the appropriate solvent and tubing. The software uses the entered
volume to calculate a calibration factor. The system uses the factor to adjust
the pumping time to ensure that the correct sample volume is always used.
Details of the calibration used are displayed on the Sipper Calibration
screen.
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Spectrophotometer User Guide
Sipper Calibration screen
This screen displays the current sipper calibration information.
To alter the calibration, press Calibrate.
* SIPPER CALIBRATION *
25/10/08 16:47
NOMINAL VOL
NO. SIPS DONE
TOTAL VOL SIPPED
TUBING CAL
:
:
:
:
1.000 ml
5
5.100 ml
1.020
VIEW
CALIB
CALIBRATE
Function key
Description
View Calib
Displays the Sipper calibration results.
Calibrate
Starts the calibration procedure and displays the Calibrate Sipper
screen.
Calibrate Sipper screen
This screen is displayed when you press Calibrate from the Sipper
Calibration screen.
Using the solvent and tubing that will be used for the sample solutions,
bring a measuring cylinder filled to the highest gradation to the sipper
uptake tube and press the switch plate.
* CALIBRATE SIPPER *
NOMINAL VOL
NO. SIPS DONE
: 1.000 ml
: 5
SIPPER
SCREEN
The sipper pumps a sample and the spectrophotometer issues a beep.
Withdraw the measuring cylinder to allow the sipper to pump the air gap.
The values used for sample volume and air gap are those set on the Sipper
screen.
Spectrophotometer User Guide 139
Repeat this process for a number of cycles up to a maximum of 10, and
then press Enter.
Measure the total volume taken from the measuring cylinder and enter this
value. The calibration appears.
140
Spectrophotometer User Guide
Function key
Description
Sipper Screen
Displays the Sipper screen and cancels the calibration.
MiniSipper
The MiniSipper is an optional accessory that enables samples to be drawn
into a flow cell for automatic measurement. The MiniSipper works with
any kind of flow cell.
After the measurement is complete, the sample is sent to a waste receptacle.
A continuous pumping mode can be used to wash the system with solvent
when required, e.g., between applications.
This section describes how to use Local Control Software to operate the
MiniSipper. For complete installation and operating instructions for this
accessory, see the corresponding manual on the documentation CD.
To operate the MiniSipper, install the accessory as described in the
operating manual. Present the sample to the MiniSipper and press the
switch to draw the required sample volume into the tubing. When the
system beeps, remove the sample to allow the system to draw in the
required air gap. When the measurement is completed, press the switch
again to empty the flow cell. The sample is pumped to a waste receptacle.
When the sipper is connected, a status box appears on the right side of
every method screen. The status box indicates the presence of the
MiniSipper and its status.
Spectrophotometer User Guide 141
Sipper screen
* SIPPER *
SIPPER
MODE
AIR GAP
SAMPLE VOL.
SAMPLE
LOW VOL.
:
:
:
:
:
:
OFF
SIP
50 cm
1.000 ml
WASTE
OFF
VIEW
CALIB
CALIBRATE
Use this screen to set up the MiniSipper for the required analysis. These
parameters are saved with any data produced by the software.
To reach this screen, press Accessories from the Home screen, then select
Sipper and press Enter. To change a parameter setting, select the parameter
and press Enter. See Parameter Entry for more information.
Item
Function
Sipper
Turns the MiniSipper on and off.
Mode
Selects the run mode for the MiniSipper.
Sip – Sets the system to fill the flow cell.
Sip&Run – Sets the system to fill the flow cell and automatically
perform a measurement. The current method is used to produce the
result (e.g., if Fixed is current, then the sample will be scanned
using the Fixed method as programmed).
Continuous – Sets the system to pump continuously to waste.
Alternate switch presses will start and stop pumping. The
instrument will not process any key presses while the MiniSipper is
pumping in continuous mode.
AutoSam – Configures the Sipper to operate with an autosampler.
Air Gap
Enter value between 0 and 500 cm.
Sets the gap between the trailing meniscus of the current sample
and the leading meniscus of the next sample. The gap is measured
to the nearest centimeter.
For best results, set the airgap to ≥8 cm from the flow cell.
Sample Vol
Enter a value between 0.5 and 10.000 ml.
Sets the volume of sample to be pumped.
142
Spectrophotometer User Guide
Item
Function
Sample
Automatically set to Waste. After measurement, the sample is
pumped through the flow cell to waste by the act of pumping the
next sample.
Low Vol
Automatically set to Off. Pumping time is set to maintain the correct
air gap for a standard internal diameter (4.0 mm) uptake tube.
Function key
Description
View Calib
Displays the Sipper Calibration screen.
Calibrate
Starts the Sipper calibration procedure.
MiniSipper Calibration
Calibration adjusts the MiniSipper for variations in pump and uptake
tubing and sample viscosities. During calibration, you enter the volume
you want to extract from the sample and the system performs several sips
using the appropriate solvent. The software uses the entered volume to
calculate a calibration factor. The system uses the factor to adjust the
pumping time to ensure that the correct sample volume is always used.
Details of the calibration used are displayed on the Sipper Calibration
screen.
Sipper Calibration screen
This screen displays the current sipper calibration information.
To alter the calibration, press Calibrate.
* SIPPER CALIBRATION *
25/10/08 16:47
NOMINAL VOL
NO. SIPS DONE
TOTAL VOL SIPPED
TUBING CAL
VIEW
CALIB
:
:
:
:
1.000 ml
5
5.100 ml
1.020
CALIBRATE
Spectrophotometer User Guide 143
Function key
Description
View Calib
Displays the Sipper calibration results.
Calibrate
Starts the calibration procedure and displays the Calibrate
Sipper screen.
Calibrate Sipper screen
This screen is displayed when you press Calibrate from the Sipper
Calibration screen.
Using the solvent that will be used for the sample solutions, bring a
measuring cylinder filled to the highest gradation to the sipper uptake tube
and press the switch.
* CALIBRATE SIPPER *
NOMINAL VOL
NO. SIPS DONE
: 1.000 ml
: 5
SIPPER
SCREEN
The sipper pumps a sample and the spectrophotometer issues a beep.
Withdraw the measuring cylinder to allow the sipper to pump the air gap.
The values used for sample volume and air gap are those set on the Sipper
screen.
Repeat this process for a number of cycles up to a maximum of 10, and
then press Enter.
Measure the total volume taken from the measuring cylinder and enter this
value. The calibration appears.
144
Spectrophotometer User Guide
Function key
Description
Sipper Screen
Displays the Sipper screen and cancels the calibration.
Calibration Verification Carousel
The Calibration Verification Carousel (CVC) measures fundamental
operating parameters to ensure the spectrophotometer is operating
according to specifications. The CVC replaces the standard cell carousel in
the spectrophotometer sample compartment.
Calibration values for the CVC are provided on a PC-format USB memory
device, which are loaded during setup.
Note
Notice
The calibration process of the CVC wavelength and absorbance filters is
accredited by the United Kingdom Accreditation Service (UKAS) to an
ISO/IEC Standard 17025 approved procedure.
We recommend that you back up the calibration data storage device before
use and store the master in a secure location.
This section describes how to install and remove the CVC and to set up
and operate the device using Local Control Software.
CVC Setup
Follow these steps to load the serial number and calibration data for the
CVC into the spectrophotometer’s internal memory:
Note
The first time you run this procedure, the message “W1022 – NVM
Checksum” is displayed. Press ESC to clear the message.
1. Press Home and select Setup.
2. Insert the USB memory device that came with the CVC in the USB
connector on the front of the spectrophotometer.
3. Select CVC and press Enter.
4. Press Load Data.
Spectrophotometer User Guide 145
The instrument loads the serial number and calibration date in the
instrument’s non-volatile memory (NVM) and displays the values in
the Calibration Data section (top portion) of the CVC Setup screen.
CVC Setup screen
* SETUP CVC *
25/10/08 16:47
CALIBRATION DATA
SERIAL NUMBER
CALIBRATION DATE
CAROUSEL
SERIAL NUMBER
SETUP
PAGE
INIT
IALIZE
Item
Function
Calibration Data
Calibration data for the standards in the CVC. These values are
loaded into the spectrophotometer memory during setup.
Serial Number
Shows the serial number of the calibration standards in the
CVC.
Calibration Date
Shows the original calibration date of the standards in the CVC.
Serial Number
Spectrophotometer User Guide
: 32764
LOAD
DATA
Carousel
146
: 32764
: 03/12/08
Calibration data for the standards in the installed CVC (read by
the spectrophotometer when you initialize).
Shows the serial number of the installed CVC.
Function key
Description
Initialize
Reads the serial number of the installed carousel and initializes
the carousel.
Load Data
Loads calibration data from installed USB memory device into
the spectrophotometer memory.
Setup Page
Displays the instrument Setup screen.
Automatic CVC calibration
You can set up the instrument to calibrate automatically on start-up if the
CVC is installed. To set this up, display the Setup screen, select
Environment and set Automatic Cal. Val. to On. When you start the
instrument, the system automatically waits for the warm-up period (60
minutes) and then performs tests 1, 2 and 3 (or tests 1 and 2 for the
AquaMate Vis spectrophotometers).
To cancel automatic calibration, press ESC.
Installing the CVC carousel
1. Remove the cover from the motor drive.
2. Remove the single cell holder or other accessory from the sample
compartment.
3. Install the CVC carousel.
Place the carousel on the motor shaft of the Smart base unit, taking
care to align the tab on the underside of the carousel with the notch on
the motor shaft.
4. Tighten the central screw by turning it clockwise.
5. From the CVC Setup screen, press Initialize to identify and align
the carousel.
6. Check that the Carousel serial number displayed on the CVC Setup
screen (bottom portion) matches the serial number of the
calibration data (top portion).
Notice
This step is important to ensure correct operation of the system. Even
though the spectrophotometer checks for a data-to-carousel match before
running any test, an initial confirmation is recommended.
Spectrophotometer User Guide 147
CVC Home screen
* CVC TEST *
TEST
STATUS
TIME
DATE
1
2
3
4
5
6
7
PASS
PASS
PASS
PASS
PASS
PASS
PASS
11
11
11
11
11
11
12
03/12/08
03/12/08
03/12/08
03/12/08
03/12/08
03/12/08
03/12/08
WAVELENGTH
ABSORBANCE
UV ABSORBANCE
STRAY LIGHT
BANDWIDTH
NOISE
DRIFT
SAVE
RESULTS
PRINT
SUMMARY
PRINT
ALL
:
:
:
:
:
:
:
05
20
25
28
33
38
40
TESTS
1-3
ALL
TESTS
The CVC Test screen lists the available tests and, for each test, reports the
time and date the previous test was run and whether it passed or failed. To
reach this screen, press Home (or General Tests) and then press Cal. Val.
To perform a specific test, select the required option(s) either individually,
using the arrow keys, or as a group, using the appropriate function keys.
Then press Run.
The software calculates the instrument and lamp hours and lamp energies
and displays them on the appropriate screen as each test is run.
Function key
Description
Save Results
Displays the Save screen, which allows you to save the current
set of test results in the instrument library or a library USB device.
Files are saved with a .TST extension.
Print Summary
Prints the summary of results as shown on the screen.
Print All
Prints the summary of results plus full details of each test result.
Tests 1-3
(or Tests 1-2)
Selects the first three tests in the list (tests 1 and 2 for the
AquaMate Vis).
Press Run to run the selected tests in sequence.
All Tests
Selects all the tests in the list.
Press Run to run the tests in sequence.
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Spectrophotometer User Guide
Each time you run a test, the instrument reads the CVC serial number and
records it with the test result. If the serial number of the installed CVC
does not match the serial number of the calibration data stored in
instrument memory, the system reports error E3083 - “Serial Numbers do
not match.”
Results screens
Each test result includes the following information:
•
•
•
•
•
•
The test number and name.
The expected values and the measured values.
The differences, tolerances, etc. (as appropriate).
The pass/fail status of the test.
The spectrophotometer and CVC serial numbers.
The instrument hours, lamp hours and lamp energies.
The following function keys are available from each Test Results screen.
Function key
Description
Test Screen
Displays the Test home screen.
Save Results
Displays the Save screen which can be used to save the test
results to a USB memory device.
Print Result
Prints the test result using the selected printer.
Stop
Cancels the current test. This option is available only while a test is
running. After you press Stop, any results obtained from the test up
to that point are discarded.
Removing the CVC carousel
1. Hold the carousel firmly and turn the central screw
counterclockwise until the carousel is released.
2. Lift the carousel off the motor shaft.
Store the CVC in its protective box.
3. Replace the cover on the motor drive unless you plan to install the
Cell Changer.
Spectrophotometer User Guide 149
Analog Data Output
Our spectrophotometers have an analog output which can be used to
provide a signal for a chart recorder. This section explains how to connect
and set up a recorder.
Connection
Use the Recorder Lead to connect the recorder to the REC port on the
spectrophotometer rear panel. This lead is used for both 0-10 mV and
0-1 V full scale deflection (fsd) chart recorders. Use the blue plug for
0-10 mV or the red plug for 0-1 V.
Note
If your chart recorder operates in either voltage range, we recommend that
you use the 0-1 V setting with the appropriate plugs (red and black).
Setup
Press Home and then press Setup. On the Setup screen, select Recorder and
press Enter to display the Recorder Setup screen.
* RECORDER *
CHART LOW (ABS)
CHART HIGH (ABS)
: -0.3000
: 6.0000
CHART LOW (%T)
CHART HIGH (%T)
: 0.0001
: 100000.0000
CHART LOW (I)
CHART HIGH (I)
: 0.0000
: 99.9999
The Chart High and Chart Low parameters set the full scale deflection on
the analog chart recorder output for each of the available measurement
modes (Absorbance, %Transmittance and Intensity). On startup, these
limits are set to the maximum measurement ranges shown above.
To reset a limit, select the parameter and press Enter to display a pop-up
entry box. Enter the new value and press Enter.
Spectrophotometer User Guide 150
Note
For best results, set Chart Low (ABS) to a small negative value (e.g., 0.1 A)
if you are working with absorbance values that are close to zero.
To operate the recorder, select the appropriate method, insert a blank
sample and press Zero. When the instrument has finished zeroing, adjust
the chart recorder so that its baseline is at the required position.
Note
The chart recorder may be driven off scale while the instrument is
zeroing.
Spectrophotometer User Guide 151
Maintenance
The information in this section deals only with those parts of maintenance
or service which can be safely carried out by the user. Work other than that
detailed must be carried out by a service engineer.
ƒ
ALWAYS ENSURE THAT BOTH THE SAMPLE AND
REFERENCE BEAMS (if double-beam instrument) ARE CLEAR
BEFORE SWITCHING ON THE INSTRUMENT. Failure to do so
will produce abnormal results.
ƒ
Low lamp energy values can be caused by leaving cells in the sample
and/or reference beams during energy measurement. ALWAYS
CHECK THAT BOTH THE SAMPLE AND REFERENCE BEAMS
(if double-beam instrument)ARE CLEAR BEFORE MEASURING
LAMP ENERGIES.
ƒ
Abnormal results will be produced if a sample is left in the beam when
Zero/Base is pressed. ALWAYS ENSURE THAT THE SAMPLE IS
REMOVED AND THAT BOTH THE SAMPLE AND
REFERENCE BEAMS (if double-beam instrument)ARE CLEAR OR
CONTAIN THE APPROPRIATE ZERO REFERENCES BEFORE
ZEROING THE INSTRUMENT OR PERFORMING A
BASELINE SCAN.
ƒ
VERY OFTEN POOR INSTRUMENT PERFORMANCE OR
FAILURE CAN BE ATTRIBUTED TO SIMPLE FAILURE OF
THE TUNGSTEN LAMP - THEREFORE REPLACE (AS BELOW)
USING THE SPARE LAMP PROVIDED BEFORE SEEKING
FURTHER ASSISTANCE.
If any fault occurs (including the above lamp failure), these are reported by
the system as an ‘Error condition’, and an ‘Exxxx’ number is generated.
Descriptive text is also included with this message.
Spectrophotometer User Guide 152
Error Codes
Detailed below are the error codes produced if:
Symptom
Error Codes
The tungsten lamp fails or is poorly aligned
E3010
E3011
E3104
E3015
E3030
The deuterium lamp fails
E3003
E3004
E3005
E3006
E3007
E3008
E3009
E3012
E3013
E3022
E3029
E3044
E3045
The beam(s) is blocked on initialization
E3027
E3056
The Cell Changer is stalled in use (or fails to initialize)
E3001
E3002
E3054
E3055
E3082
E3084
The sample compartment is open
E3053
E3062
E3068
E3069
E3071
A comprehensive list of these codes is available in the service manual for
these products. Generation of a code not related to replacement of either
the tungsten or deuterium lamps usually requires you to contact technical
support.
Spectrophotometer User Guide 153
Routine maintenance
Very little maintenance is required to keep the spectrophotometer in good
working condition. The interior should be kept as dust free as possible and
the sample compartment cleaned regularly; wipe off spilt chemicals
immediately.
Replacement sample compartment liners are available.
Cleaning the instrument exterior
The exterior of the instrument can be cleaned periodically as follows:
Caution
Do not allow moisture to leak into the instrument.
1. Turn off the spectrophotometer and disconnect it from the main
power supply.
2. Wipe the outside surface of the instrument, as necessary, with a
clean, lint-free cloth dampened with a weak solution of detergent
and water.
3. Wipe the same areas with a cloth dampened with plain water.
4. Dry the surface with a clean, dry cloth.
154
Spectrophotometer User Guide
Removal and replacement of the tungsten-halogen lamp
Warning
Avoid shock hazard. Turn off and disconnect the spectrophotometer from
the main power supply and allow the lamp to cool for at least 15 minutes
before proceeding.
1. Turn off the spectrophotometer power and unplug the power
supply from the wall outlet or power strip.
Caution
Allow at least 15 minutes for the instrument to cool before removing
covers.
2. Remove the back corner cover.
Turn the fastener one-quarter turn counterclockwise and slide the cover
up to remove it.
3. Remove the metal housing.
Grab the sides of metal housing and pull upwards.
4. Remove the burned out bulb from its socket.
a. Remove spring clip.
b. Lift the bulb straight up.
Spectrophotometer User Guide 155
Caution
Never touch a bulb with your fingers. Oil from your skin will cause the
bulb to burn out quickly or explode.
5. Use clean finger cots or a clean laboratory tissue to pick up the bulb
and insert the pins into the socket.
Note
These lamps are manufactured to very high tolerances. However, to ensure
optimum energy throughput, align the lamp filament exactly as shown in
the diagram (with the white line on the lamp base facing towards the front
of the instrument).
6. Replace the spring clip.
7. Replace metal housing and rear cover.
8. Plug in the spectrophotometer power cord and turn on the power
switch.
9. Use the Local Control Software to reset the Lamp hours and energy
(if applicable).
156
Spectrophotometer User Guide
Removal and replacement of the deuterium lamp
A deuterium lamp is included with all instrument models except the
AquaMate Vis.
Warning
Avoid shock hazard. Turn off and disconnect the spectrophotometer from
the main power supply and allow the lamp to cool for at least 15 minutes
before proceeding.
Warning
UV radiation from a deuterium lamp can be harmful to the skin and eyes.
Always view the lamp through protective glasses that will absorb UV
radiation. Avoid looking directly at the deuterium arc. Do not expose the
skin to direct or reflected UV radiation.
1. Turn off the spectrophotometer power and unplug the power
supply from the wall outlet or power strip.
Caution
Allow at least 15 minutes for the instrument to cool before removing
covers.
2. Remove the back corner cover.
Turn the fastener one-quarter turn counterclockwise and slide the cover
up to remove it.
Spectrophotometer User Guide 157
3. Disconnect the lamp at the in-line connector.
4. Using the key provided, loosen the three locating screws, rotate the
lamp assembly counterclockwise and lift the lamp out of the
instrument.
Notice
When installing the deuterium lamp avoid handling the silica envelope.
Finger marks become burnt on and cannot be removed after the lamp is
switched on. This can affect the light output characteristics. Handle only
the base of the lamp or the mounting plate. If the silica envelope becomes
contaminated, clean with a powerful degreasing solvent such as absolute
alcohol before the lamp is switched on.
5. Hold the new lamp and locate the notch in the mounting plate.
6. Position the lamp so that the notch points towards the lamp change
mirror.
7. Use the provided key to tighten the locating screws.
8. Re-connect the new lamp at the in-line connector.
9. Replace the rear cover.
158
Spectrophotometer User Guide
10. Connect the spectrophotometer to the mains supply and turn it on.
11. Allow half an hour for the instrument to warm up.
12. Use the Local Control Software to reset the Lamp hours.
Spectrophotometer User Guide 159
BioMate 6 Test Parameters
The following table lists the parameters for the BioMate Tests. The list
includes a brief description of each parameter, the applicable range and the
initial default values. Use this list as a reference when setting up tests.
Parameter
Description
Initial Default Values
Range
%Formamide
Percentage of formamide contained in the
sample
0
1-100
Integer
%GC
Percentage of GC pairs contained in the
sample
empty; not an entry
NA
%Mismatched
Percentage of mismatched bases in the
sample
0
1-100
Integer
Auto Print
Turns automatic printout on or off
Off
On↔Off
Base Sequence
Sequence of bases contained in the
sample
empty field, unless sequence
entered previously
40 characters max.
Cation Molarity
Molarity of cation (Na) contained in the
sample
0.050
0.001-9999
Curve Fit
Type of Line fit calculation
Std Curve: Linear
Linear
Bradford-Standard: Quadratic
Linear Through Zero
Bradford-micro: Quadratic Lowry- Quadratic
Standard: Quadratic Pierce LowryQuadratic Through Zero
micro: Quadratic
BCA-Standard: Quadratic
Pierce Modified BCA: Quadratic
Biuret: Linear Through Zero
Date Standards
Measured
Date when standards were last measured
with this instrument
------
Diluent Volume
Volume of diluent added prior to
measurement
0
0-999
Integer
Spectrophotometer User Guide 160
Parameter
Description
Initial Default Values
Range
Dilution Multiplier
Factor used to correct for sample dilution
1.00
1.00-99999
x.xx
xx.xx
xxx.x
xxxx
xxxxx
Display Protein
Indicates whether results should appear
as units of protein concentration
Off
On↔Off
DNA ε(260)
Extinction coefficient
empty; not an entry
NA
DNA Mol. Wt.
Molecular weight of DNA contained in
sample
empty; not an entry
NA
Factor
See individual calculations for usage
DNA (260/280), DNA with Scan
(260/280):
-0.001 to -99999;
0.001 to 99999
DNA Factor @260: 50
DNA Factor @280: 0
x.xxx
Protein Factor @260: 757.3
xx.xx
Protein Factor @280: 1552
xxx.x
DNA (260/230), DNA with Scan
(260/230):
xxxx
xxxxx
DNA Factor @260: 50
DNA Factor @230: 0
Protein Factor @260: 75.8
Protein Factor @230: 183
ssDNA: 50
RNA: 40
Direct UV-Oligos : 33
Direct UV (280): 1
Direct UV (205): 38
Oligo calculator: blank if no base
sequence entered; calc value if
base sequence entered
Warburg-Christian: 1.55 @ 280;
0.76 @ 260
ID#
Numeric Identifier – autoincrements
during test until reset or test is exited
1
0=OFF
1-999
Integer
Spectrophotometer User Guide 161
Parameter
Description
Initial Default Values
Range
Low/High Limits
Lowest & highest acceptable results,
outside of which the result is flagged as
‘Low’ or ‘High’
-9999/9999
±9999
Number of Bases
Number of bases contained in sample
empty; not an entry
NA
Number of Samples
Number of samples to be measured in the
whole test
1
1-999
Integer
Bradford-micro, Lowry, Biuret: 6
1-20
BCA, Bradford-std: 8
Integer
(if cell changer installed)
Number of Standards
Number of standards to be measured for
calibration curve
Pierce Lowry: 9
Ref. Wavelength
Internal Reference wavelength; for each
reported measurement, measures
analytical wavelength & reference
wavelength.
DNA: 320
190-1100
On↔Off
Reported measurement = abs @ analytical
WL – abs @ Reference WL
Ref. Wavelength
Correction
Turns internal zeroing on or off
Off
Sample Positioner
Manual = moved by buttons
Auto 6 +Ref if 7 Cell Changer fitted Auto 7 / Auto 6 + Ref. /
Manual 7 for 7 Cell
Not displayed if Single Cell Holder Changer;
fitted
no choice for Single Cell
Holder
Auto 6 + Ref = auto moved –Ref, 2,3,4,5,6,7
Auto 7 = auto moved –1,2,3,4,5,6,7
Sample Volume
Total volume of sample
1
1-999
Integer
Scan Start
Start wavelength for scan
225.0nm
190.0 – 1100.0
Scan Stop
Final wavelength for scan
325.0nm
190.0 – 1100.0
162
Spectrophotometer User Guide
Parameter
Description
Initial Default Values
Range
Standard
Concentrations
Concentration of standards used to
generate standard curve for the test
Coomassie/Bradford-Std: 25, 125,
250, 500, 750, 1000, 1500, 2000
0.000-9999
Bradford-micro: 2, 5, 10, 15, 20, 25
Lowry-Std: 0, 100, 200, 500, 1000,
2000
Lowry-micro: 1, 5, 25, 125, 250, 500,
750, 1000, 1500
BCA-Std: 25, 125, 250, 500, 750,
1000, 1500, 2000
BCA-micro: 0, 0.5, 1, 2, 5, 10, 20, 40,
200
Biuret: 0, 2, 4, 6, 8, 10
Statistics
Turns statistics function on or off; if ON,
calculates average and Std Dev of results;
Off
On↔Off
Test Name
Defined by user to identify stored tests
DNA (260/280)
up to 19 characters
DNA (260/230)
DNA WITH SCAN
ssDNA
RNA
DNA/RNA (260)
OLIGOS (FACTOR)
OLIGOS (CALC)
COOMASSIE/BRADFORD-STD
COOMASSIE/BRADFORD-MICRO
LOWRY-STANDARD
PIERCE MODIFIED LOWRY
BCA-STANDARD
PIERCE MICRO BCA
BIURET
DIRECT UV (280)
DIRECT UV (205)
WARBURG-CHRISTIAN
CELL GROWTH
Spectrophotometer User Guide 163
Parameter
Description
Initial Default Values
Range
Tm values
Predicted melting point temperatures
empty; not an entry
NA
Units
Labels concentration results
DNA Ratio: μg/ml for DNA &
Protein
Up to 9 characters
DNA Scan: μg/ml for DNA &
Protein
ssDNA: μg/ml
RNA: μg/ml
Oligos: μg/ml
Bradford Protein: μg/ml
Lowry Protein: μg/ml
BCA-Standard Protein: mg/ml
BCA-micro: μg/ml
Biuret Protein: mg/ml
Direct UV Protein: mg/ml
Warburg-Christian: mg/ml
Wavelength values
Values for the analytical wavelengths
DNA: 260, 280; 260, 230
dsDNA, ssDNA, RNA: 260
DNA scan: 225, 325
Oligos (entered factor): 260
Oligos (calc factor): 260
Bradford-Standard & -micro: 595
Lowry-Standard: 550
Lowry-micro: 750
BCA-Standard & -micro: 562
Biuret: 540
Direct UV: 280
Direct UV: 205
Warburg-Christian: 260, 280
Cell Growth: 600
164
Spectrophotometer User Guide
190.0-1100.0nm
Calculations for BioMate 6 Tests
Test Name
Test Types
Calculation (s)
Default Parameters
Displayed Units
Absorbance
Difference +
Absorbance
Ratio
Dilution Factor (Df ) =
A1 = 260nm
A2 = 280nm
Aref = 320nm
(optional)
f1 = 50
f2 = 0
f3 = 1552
f4 = 757.3
dil.vol. = 0
smp.vol = 1
DNA: μg/ml
A1 = 260nm
A2 = 230nm
Aref = 320nm
(optional)
f1 = 50
f2 = 0
f3 = 183
f4 = 75.8
dil vol. = 0
smp.vol = 1
DNA: μg/ml
Start wavelength =
230nm
Stop wavelength =
330nm
None
Nucleic Acid Tests
DNA/Protein
concentration and
DNA Purity (260, 280)
diluent vol + sample volume
sample volume
DNA concentration =
[(A1 – Aref)f1 – (A2 – Aref)f2] Df
Protein concentration =
[(A2 – Aref)f3 – (A1 – Aref)f4] Df
Protein: μg/ml
Ratio: no units
Ratio = A1 - Aref
A2 - Aref
DNA/Protein
concentration and
DNA Purity (260, 230)
Absorbance
Difference +
Absorbance
Ratio
Dilution Factor (Df ) =
diluent vol + sample volume
sample volume
DNA concentration =
[(A1 – Aref)f1 – (A2 – Aref)f2] Df
Protein concentration =
[(A2 – Aref)f3 – (A1 – Aref)f4] Df
Protein: μg/ml
Ratio: No units
Ratio = A1 - Aref
A2 - Aref
DNA/Protein
concentration and
DNA Purity (260, 280)
with SCAN
Scan –
Absorbance
None
Spectrophotometer User Guide 165
Test Name
Test Types
Calculation (s)
Default Parameters
Displayed Units
Absorbance
Difference +
Absorbance
Ratio + Scan
Dilution Factor (Df ) =
A1 = 260nm
A2 = 280nm
Aref = 320nm
(optional)
f1 = 50
f2 = 0
f3 = 1552
f4 = 757.3
dil.vol. = 0
smp.vol. = 1
DNA: μg/ml
diluent vol + sample vol.
sample volume
DNA concentration =
[(A1 – Aref)f1 – (A2 – Aref)f2] ] Df
Protein concentration =
[(A2 – Aref)f3 – (A1 – Aref)f4] ] Df
Protein: μg/ml
Ratio = A1 - Aref
A2 - Aref
DNA/Protein
concentration and
DNA Purity (260, 230)
with SCAN
Scan –
Absorbance
None
Start wavelength =
230nm
Stop wavelength =
330nm
None
Absorbance
Difference +
Absorbance
Ratio + Scan
Dilution Factor (Df ) =
A1 = 260nm
A2 = 230nm
Aref = 320nm
(optional)
f1 = 50
f2 = 0
f3 = 183
f4 = 75.8
dil.vol. = 0
smp.vol. = 1
DNA: μg/ml
260nm, FactordsDNA =
33
dil.vol. = 0
smp.vol. = 1
μg/ml
260nm,FactorssDNA/
ssRNA =40
dil.vol. = 0
smp.vol. = 1
μg/ml
260nm,FactorssDNA/
=50
dil.vol. = 0
smp.vol. = 1
μg/ml
diluent vol + sample vol.
sample volume
DNA concentration =
[(A1 – Aref)f1 – (A2 – Aref)f2] ] Df
Protein concentration =
[(A2 – Aref)f3 – (A1 – Aref)f4] ] Df
Protein: μg/ml
Ratio = A1 - Aref
A2 - Aref
Direct UV – ssDNA
Factor
Dilution Factor (Df ) =
diluent vol + sample vol.
sample volume
Conc. = (F x A260)Df
Direct UV – RNA
Factor
Dilution Factor (Df ) =
diluent vol + sample vol.
sample volume
Conc. = (F x A260)Df
Direct UV – DNA/RNA
Factor
Dilution Factor (Df ) =
diluent vol + sample vol.
sample volume
166
Spectrophotometer User Guide
ssRNA
Test Name
Test Types
Calculation (s)
Default Parameters
Displayed Units
260nm
dil.vol. = 0
smp.vol. = 1
μg/ml
260nm
Factoroligos = 38
dil.vol. = 0
smp.vol. = 1
μg/ml
Conc. = (F x A260)Df
Direct UV – oligos
(w/base calculator)
Factor
Dilution Factor (Df ) =
diluent vol + sample vol.
sample volume
Conc. = (F x A260)Df
F = factor calculated by Oligo Calculator
Direct UV - oligos
Factor
Dilution Factor (Df ) =
diluent vol + sample vol.
sample volume
Conc. = (F x A260)Df
Protein Tests
Coomassie/Bradford
– standard
Standard
Curve
Second order
595 nm
Standard
concentrations of
25, 125, 250, 500, 750,
1000, 1500, 2000
μg/ml
Coomassie/Bradford
– micro
Standard
Curve
Second order
595 nm
Standard
concentrations of
2.5, 5, 10, 15, 20, 25
μg/ml
Lowry – standard
Standard
Curve
Second order
550 nm
Standard
concentrations of 0,
100, 200, 500, 1000,
2000
μg/ml
Pierce-Modified
Lowry – micro
Standard
curve
Second order
750 nm
Standard
concentrations of 1,
5, 25, 125, 250, 500,
750, 1000, 1500
μg/ml
BCA (Bicinchoninic
Acid) – standard
Standard
Curve
Second order
562 nm
Standard
concentrations of
25, 125, 250, 500, 750,
1000, 1500, 2000
mg/ml
Pierce Micro BCA
Standard
Curve
Second order
562 nm
Standard
concentrations of
0.5, 1, 2.5, 5, 10, 20,
40, 200
μg/ml
Spectrophotometer User Guide 167
Test Name
Test Types
Calculation (s)
Default Parameters
Displayed Units
Biuret
Standard
Curve
First order through zero
540 nm
Standard
concentrations of 0,
2, 4, 6, 8, 10
mg/ml
Direct UV (280)
Factor
Dilution Factor (Df) =
280 nm, Factor280 = 1
dil.vol. = 0
smp.vol. = 1
mg/ml
205nm, Factor205 = 31
dil.vol. = 0
smp.vol. = 1
mg/ml
A1 = 280nm
A2 = 260nm
f1 = 1.55
f2 = 0.76
mg/ml
600nm
Abs
diluent vol + sample vol.
sample volume
Conc. = (F x A280)Df
Direct UV (205)
Factor
Dilution Factor (Df) =
diluent vol + sample vol.
sample volume
Conc. = (F x A205)Df
Warburg-Christian
Absorbance
difference
Dilution Factor (Df) =
diluent vol + sample vol.
sample volume
Protein Concentration = [A1.f1 –A2.f2] Df
Cell Growth Test
Cell growth
168
Absorbance
Spectrophotometer User Guide
None
BioMate Oligo Calculator
Calculation Name
Entry Parameters
Formula
Displayed Units
# of bases
Repetitive sequence of A, T (or U),
G and C
Count of total # of bases entered
Length = # of bases
%GC content
Use AT(U)GC sequence entered
above
%GC = # of (G + C) bases x 100
Percentage
# units A, # units T, # units G, # units
C, # units U
If entry does not include U:
Molecular weight
total # of AT(or U)GC
MW = (312.2 x A) + (303.2 x T) + (329.2 x G)
+ (289.2 x C) + 18.02
Molecular weight = x
daltons/M
If entry does include U:
MW = (329.2 x A) + (306.2 x U) + (345.2 x G)
+ (305.2 x C) + 18.02
Absorptivity
# units A, # units T, # units G, # units
C, # units U
If entry does not include U:
ε260 = (15,200 x A) + (8,400 x T) + (12,010 x
G) + (7,050 x C)
Extinction coefficient
= M-1cm-1
If entry includes U:
ε260 = (15,200 x A) + (9,900 x U) + (12,010 x
G) + (7,050 x C)
Factor
N/A
Molecular Weight x 103
μg/mL
Extinction Coefficient
Calculation of Tm:
Oligos up to 40-mers
in length
Calculation of Tm:
DNA-DNA hybrids
# units A, # units T, # units G, # units
C, # units U
Tm = 2(A + T (or U)) + 4(G + C)
°C
# units A, # units T, # units G, # units
C
Tm = 81.5 + 16.6log10 ((M)/ (1+0.7(M)) +
0.41(%GC) - 500/L - P - 0.63(%form)
°C
M = molarity of Na+
%GC = percentage of G and C
%form = %formamide in the
solution
L = # of base pairs
P = % mismatching
Spectrophotometer User Guide 169
Calculation Name
Entry Parameters
Formula
Displayed Units
Calculation of Tm:
# units A, # units T, # units G, # units
C, # units U
Tm = 67 + 16.6log10((M)/
°C
DNA-RNA hybrids
M = molarity of Na+
(1+0.7(M)) + 0.8(%GC)-500/L - P
- 0.5(%form)
%GC = percentage of G and C
%form = %formamide in the
solution
L = # of base pairs
P = % mismatching
Calculation of Tm:
RNA-RNA hybrids
# units A, # units G, # units C, #
units U
Tm = 78 + 16.6log10((M)/ (1+0.7(M))
+0.7(%GC)-500/L-P-0.35(%form)
°C
pmol/μl = (conc x 1000)/ mol.wt.
pmol/μl
M = molarity of Na+
%GC = percentage of G and C
%form = %formamide in the
solution
L = # of base pairs
P = % mismatching
Conversion from
μg/ml to pmol/μl
170
conc = concentration (μg/ml)
mol.wt. = sequence molecular
weight
Spectrophotometer User Guide