Nikon Optiphot Instructions
Microscope Summary
The ImageCORE’s Nikon Optiphot-2 is set up as a digital fluorescence
acquisition microscope. The Eclipse is coupled to a 12-bit Roper Coolsnap CF digital
capture camera. This system should be used to observe and record images prior to use of
other, higher resolution, ImageCORE resources.
Power Up Procedure
• For your protection as well as the equipment, please dawn gloves provided to you
by the ImageCORE at no charge
• Carefully remove the protective dust cover from the microscope ensuring that the
back of the cover does not catch or move the mercury supply adjustments
• The Nikon Optiphot-2 has 2 power supplies. The order in which these supplies
are turned on is important. Begin by turning on the main power supply (1.1). The
slider (1.2) located next to the power switch is used for white light source
intensity adjustment.
• Should you desire fluorescence excitation, you will need to turn on the mercury
light source. It is imperative that the mercury source is used for duration of at
least 15minutes. Likewise, it is also imperative that the mercury source not be
switched on after prior use for at least 30 minutes.
• In order to illuminate the mercury light source, turn on the power supply (1.3).
Next, in order to ignite the bulb, press and hold the “ignition” button (1.4). Once
the bulb is properly ignited, the “ready” light will illuminate.
Nikon Optiphot
Image Acquisition
• Select the appropriate excitation wavelength using the filter cube selection slider
(2.1). Further compose your image using the focus and translation stage. When
you are not actively observing or imaging your sample, it is important to engage
the shutter (2.2) and block the excitation light to your sample. When the shutter is
depressed, the light path is undisturbed and excitation can occur. Subsequently,
when the shutter is pulled out, the light path is disturbed.
• In order to set the scope for digital capture, it is necessary to manipulate the
“eyepiece/camera” selector (2.3). When depressed, the eyepieces are in the light
path and when pulled out, the digital camera is in the light path.
Having configured the microscope properly as discussed herein, it is now time to
configure the computer system for digital image capture. Please refer to the
separate instruction document entitled “Computer Login/Image Acquisition
Nikon Optiphot
Shutdown Procedure
After properly restarting the computer system as discussed in the “Computer
Login/Image Acquisition Instructions,” attention must be paid to the microscope.
Begin by returning all settings on the scope to their respective “off” positions.
This includes moving the filter slider (2.1) left to the first position. Next make
sure the shutter (2.2) is pulled out from the scope and blocking the light path.
Lastly, move the “eyepiece/camera” selector (2.3) inward towards the scope to
“eyepiece” position.
Having returned the microscope settings to their respective “off” positions, you
may now shut down the power supplies for the microscope and mercury lamp.
Again, the order in which you shut down the supplies is important.
Begin moving the light intensity slider (1.2) to the left or lowest intensity.
Next, turn off the microscope power supply (1.1).
Lastly, switch off the mercury lamp supply (1.3).
After both power supplies have been shutdown and the computer has been
restarted, please fill out the log sheet located to the left of the microscope.
Allow 15 minutes for the microscope and lamps to cool prior to replacing the dust
cover. Again, take care to ensure the dust cover is draped over the scope without
impeding the rear lamp housings (see below).
Nikon Optiphot