LS
AE31E
LIVE CELL MICROSCOPE PLATFORM
02
CONTENTS AE31E CATALOGUE
INTRODUCTION
02
THE MECHANICS
04
THE OPTICS
06
THE ILLUMINATION
08
THE ACCESSORIES
10
DOCUMENTATION
12
SPECIFICATIONS
14
The AE31 Elite is Motic’s new statement for a professional inverted microscope
providing best image quality, ergonomic design and clever illumination
features. The AE31E is the perfect platform for all kind of routine microbiology
applications in clinical and pharmaceutical laboratories with best options for
university research environments.
In full accordance with Motic’s CCIS© Infinity System, the new LWD Plan
Achromatic objectives with an intelligent Phase contrast concept deliver a
remarkable image quality. Phase contrast is an integral part of all standard
packages, while the optional 4X Phase objective is dedicated to fast screening.
The AE31E stand carries an encoded 5-fold nosepiece with a light memory
function. The illumination intensity for each objective position is memorized
and replicated once the respective objective is swung in again. Rich illumination
power is given by the 30 Watt Halogen light source in a Full-Koehler configuration, simply interchangeable with 3W LED modules of different color temperatures. For maximum power requests, a 100W Halogen version is available.
The Auto ON/OFF function, based on a built-in IR-sensor, deactivates the
microscope in case it is left by the user; no need for a final instrument check
when work is finished.
An upgrade with fluorescence is offered for the entry level of research applications. Documentation is facilitated by the 100:0/0:100 light split option for
shortest possible exposure times.
The complete AE31E microscope is manufactured in compliance with European
RoHS standards to avoid contact with lead-containing materials. An anti-fungus
treatment is applied to all parts of the microscope to protect the system in
humid environments.
03
AE31E
LIVE CELL MICROSCOPE PLATFORM
04
THE MECHANICS
STAND & STAGE | EYEPIECE TUBES
05
INTRODUCTION
THE MECHANICS
THE OPTICS
THE ILLUMINATION
THE ACCESSORIES
DOCUMENTATION
SPECIFICATIONS
SMART FUNCTIONALITY WITH
ERGONOMIC BASIS
Motic’s AE31E displays maximum functionality and ergonomics in all its mechanical aspects. Elegant
and robust, the microscope stand follows all needs for an intense daily use. The fixed stage plate with
optional lateral extensions carries a glass or metal insert for quick overview and comfortable change of
objectives. An attachable x/y object guide for a convenient sample evaluation under high magnifications
is available.
The fine focus with a step size of 2 microns allows a smooth drive through cell cultures by the
Long-Working-Distance objectives. For best possible documentation of fluorescence images, a
100:0/0:100 trinocular light split for maximum luminous efficiency can be supplied.
The AE31E eyepiece tubes guarantee a fatigue-free work in terms of viewing angle and improved
interpupillary distance, while the “butterfly” movement may increase the viewing height by 60mm for
individual positioning.
EYEPIECE TUBES | Extended “Butterfly” adjustment
METAL STAGE INSERT
GLASS STAGE INSERT
06
THE OPTICS
OBJECTIVES | EYEPIECES
07
INTRODUCTION
THE MECHANICS
THE OPTICS
THE ILLUMINATION
THE ACCESSORIES
DOCUMENTATION
SPECIFICATIONS
NEW LWD PLAN ACHROMATS
FOR EFFICIENT WORK
Motic’s new LWD Plan Achromatic objectives for inverted microscopes carry a clever concept for Phase
contrast. One illumination ring (Ph1) covers the objective magnifications from 10X up to 40X: no need
to move the Phase ring slider. The optional 4X Phase lens needs Phase ring Ph0 and is dedicated to
quick and efficient sample screening.
An optimized multi-layer coating for improved contrast as well as carefully selected glass quality for
better transmission leads to significantly brighter images. Motic is pleased to offer this newly developed
optics following the RoHS standard for lead-free manufacturing.
All optical components cooperate for a perfect image: condenser, objectives, tube lens and eyepieces.
Since the intermediate image is already fully corrected, no colored fringes will affect professional digital
results.
PLAN ACHROMAT PHASE OBJECTIVES (PL & LWD PL)
08
THE ILLUMINATION
LIGHT SOURCES | LIGHT MEMORY FUNCTION
09
INTRODUCTION
THE MECHANICS
THE OPTICS
THE ILLUMINATION
THE ACCESSORIES
DOCUMENTATION
SPECIFICATIONS
A CLEVER AND POWERFUL
LIGHT MANAGEMENT
To utilize the potentials of Motic’s new developments, the illumination concept has to take care of power
and quality. All stand versions carry the Full-Koehler setup. The 30 Watt Halogen light source is standard
in the AE31E and displays a full interchangeability with 3 Watt LED modules of different color temperatures
(4500K, 6000K). The 100 Watt Halogen version is recommended for maximum illumination power.
The light memory function of the AE31E is based on an encoded 5-fold nosepiece which memorizes the
light intensity setting for each nosepiece position. The system replicates this intensity setting once the
respective position is swung in again. Any new adjustment is taken as an overwriting of the former setup.
The built-in IR-sensor activates the Auto ON/OFF function in case the microscope is left for more than
15 minutes and reactivates the microscope by return of the user. From an environmental perspective,
this feature saves energy and increases lab safety.
100 WATT HALOGEN VERSION
10
THE ACCESSORIES
CONTRAST METHODS | FLUORESCENCE
11
INTRODUCTION
THE MECHANICS
THE OPTICS
THE ILLUMINATION
THE ACCESSORIES
DOCUMENTATION
SPECIFICATIONS
NEW TECHNOLOGIES
AND WELL-KNOWN TRADITION
The basic packages of the AE31E already offer the full functionality of a microbiology working platform.
An immediate start of work is possible, keeping further add-on options in mind. Phase contrast from
10X to 40X objective magnification is an integral part of all standard packages. The easy upgrade with
fluorescence rests upon the established fluorescence attachment with slider mechanism.This device
may carry up to 3 filter cubes. A complete range of filter combinations is available, covering routine
applications from UV up to NIR. Equipped with band pass barrier filters, multi color stainings can also
be treated.
The 100 Watt HBO lamp house with integrated mirror for increased light intensity offers a powerful
excitation for brighter signals. In combination with a Moticam digital camera, excellent image results
are ensured.
MECHANICAL STAGE
FILTER CUBES SLIDER MECHANISM
3-POSITION PHASE SLIDER
12
DOCUMENTATION
STANDARD PHOTOMICROGRAPHY | DIGITAL DOCUMENTATION
13
INTRODUCTION
THE MECHANICS
THE OPTICS
THE ILLUMINATION
THE ACCESSORIES
DOCUMENTATION
SPECIFICATIONS
REPRODUCIBLE DOCUMENTATION
RESULTS WITH HIGH RELIABILITY
Documentation is a key issue in every aspect of microscopy, with special importance in biomedical
applications like microbiology. The AE31E allows to follow this requirement by traditional photomicrography or more flexible C-mount solutions. Traditional photomicrography requires the trinocular version of the
AE31E, offering a 100:0/0:100 trinocular light split for shortest possible exposure times. This setup
delivers high resolution images of small fields. Live images for presentation and discussion may be
supplied by the software of the SLR camera manufacturer.
A more convenient approach is provided by Motic’s philosophy of easy digitization. The combination of
the AE31E with a member of Motic’s camera series delivers excellent live images which can easily be
presented to a larger public and saved for future analysis. All Moticams come equipped with a proprietary software package to transform the AE31E into an analysis and documentation station. For limited
bench-top space, the model Moticam 580 is ideal for the presentation of High Definition images on an
HDMI screen without a computer. Motic’s new tablet cameras allow a quick sample inspection without
having a look through the eyepieces.
MOTICAM TABLET SOLUTIONS
20
14
SPECIFICATIONS
AE 31E
STANDARD CONFIGURATION
& OPTIONAL ACCESSORIES
General Specifications
Optical system
Observation system
Interpupilary distance (mm)
Eyepieces
Eyepieces diopter adjustment
Optional Configuration
CCIS®
Swiveling 360° with 45° inclination
48-75
N-WF 10X/22
N-WF 12.5X/18; N-WF 15X/16
+/- 5 dpt
Reticles (Ø25mm)
Crosshair
10mm/100 divisions
Crossed double scale 10mm/100 divisions
Trinocular light split
Nosepiece
Plan Achromat objectives
Objective mounting thread RMS standard (W 4/5'' X 1/36'')
Phase ring
Size stage plate (mm)
20/80
Quintuple, side orientated
4X/0.10; Ph 10X/0.25; LWD Ph 20X/0.3; LWD Ph 40X/0.5
Focus mechanism
Fine focus precision (µm)
Z-axis movement (mm)
Illumination
Halogen / LED interchangeability
Illumination position
Ph1 - universal phase ring from 10X up to 40X
Yes
ELWD condenser N.A. 0.3 (WD 72mm)
2
10
30W Halogen/3W LED; 100W Halogen
Only in 30W version
Built-in
Auto ON/OFF
Yes
Light memory
Yes
Transformer
30W Halogen/3W LED Built-in; 100W external
Dimensions (mm)
Weight (Kg)
LWD condenser N.A. 0.5 (WD 28mm)
Coaxial; tension adjustment
Yes
Filters
Ph0 - for Ph 4X
200 x 239
Koehler
Power supply
Ph 4X/0.10; 10X/0.25; LWD 20X/0.3; LWD 40X/0.5
Yes
Attachable x/y stage with inserts
Condenser
0/100
110-240V (CE)
Blue, Ground glass, Green
Didymium, ND filters
556 x 200 x 529 for 30W; 629 x 200 x 595 in 100W
11,7 (30W); 13,2 (100W)
Contrast techniques
Brightfield
Yes
Phase contrast
Yes
HBO Fluorescence
Yes
15
SPECIFICATIONS
209
363
529
AE 31E
SCHEMATIC DIAGRAMS (units: mm) & HBO FLUORESCENCE DIAGRAM
328
100
509
200
383
564
100W mercury lamp housing
UV protection screen
Fluorescence attachment
Fluorescence filter modules
Fluorescence light filter operation slide
Starter unit HBO 100W
Lamp centering tool
SPECIFICATIONS
AE 31E
SYSTEM DIAGRAM
Neutral density filter with mount (Ø45)
Phase
Phase
Phase
Phase
stop
stop
stop
stop
0.3
0.3
0.5
0.5
Ph0
Ph1
Ph0
Ph1
Blue filter (Ø45)
Green filter (Ø45)
Ground glass filter (Ø45)
Didymium filter (Ø45)
3-position phase slider
*100W Halogen lamp house
LWD condenser
N.A. 0.5 (WD=28mm)
ELWD condenser
N.A. 0.3 (WD=72mm)
3W LED module
6000K
4500K
30W halogen lamp
Phase
centering
telescope
Eyepieces N-WF 10X/22
Eyepieces N-WF 12.5X/18
Eyepieces N-WF 15X/16
4X
Photo eyepiece
2.5X
Photo eyepiece
Auxiliary stage
Binocular tube
Allen
Hex key
Mechanical stage
Metal stage insert
Glass stage insert
Photo adapter
(to be used with
Photo eyepiece)
C-mount
adapter 1X
C-mount
adapter 0.65X
Wellplate holder
128 x 86mm
C-mount
adapter 0.5X
Hemacytometer
holder 36 x 76mm
35mm
Petri dish holder
Wellplate holder
132 x 88mm
65mm
Petri dish holder
54mm
Petri dish holder
CCIS® Plan Achromat PL 4X
*Trinocular tube
CCIS® Plan Achromat PL 10X
CCIS® Plan Achromat LWD PL 20X
CCIS® Plan Achromat LWD PL 40X
CCIS® Plan Achromat Phase PL Ph0 4X
CCIS® Plan Achromat Phase PL Ph1 10X
CCIS® Plan Achromat Phase LWD PL Ph1 20X
CCIS® Plan Achromat Phase LWD PL Ph1 40X
*Tube version (binocular; trinocular) as well a illumination (30W Hal/3W LED; 100W) are integral part of the respective microscope model and not interchangeable.
HIGHLIGHTS
OBJECTIVES | PHASE CONTRAST | ILLUMINATION
OBJECTIVES
New LWD Plan Achromatic objectives
Significantly brighter images thanks to an
optimized multi-layer coating as well as
carefully selected glass quality.
PHASE CONTRAST
New Phase Contrast concept
No need to move the phase ring slider, one
illumination ring (Ph1) covers the objective
magnifications from 10X up to 40X.
ILLUMINATION
Light memory function
The encoded 5-fold nosepiece memorizes
the illumination intensity for each objective
position, no need to adjust it again.
Canada | China | Germany | Spain | USA
www.motic.com
EN | ES | FR | DE | IT | PT
Motic Instruments Inc. (Canada)
130 - 4611 Viking Way. Richmond, BC V6V 2K9 Canada
Tel: 1-877-977 4717 | Fax: 1-604-303 9043
Motic Deutschland GmbH (Germany)
Christian-Kremp-Strasse 11, D-35578 Wetzlar, Germany
Tel: 49-6441-210 010 Fax: 49-6441-210 0122
Motic Hong Kong Limited (Hong Kong)
Rm 2907-8, Windsor House, 311 Gloucester Road, Causeway Bay, Hong Kong
Tel: 852-2837 0888 | Fax: 852-2882 2792
Motic Spain, S.L. (Spain)
Polígon Industrial Les Corts, Camí del Mig, 112 08349 Cabrera de Mar, Barcelona, Spain
Tel: 34-93-756 6286 | Fax: 34-93-756 6287
Official Distributor:
*CCIS® is a trademark of Motic Incorporation Ltd.
Motic Incorporation Limited Copyright © 2002-2015. All Rights Reserved.
Design Change: The manufacturer reserves the right to make changes in instrument design in
accordance with scientific and mechanical progress, without notice and without obligation.
Designed in Barcelona (Spain)
June 2015
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