Microscope Instruc Manual 7 Full .pub

BIOMEDX
OPERATING
INSTRUCTION
MANUAL
Fiberoptic Video
Microscope System
www.biomedx.com
BIOMEDX
FIBEROPTIC MICROSCOPE SYSTEM
Instruction Manual
This instruction manual is for the Biomedx Fiberoptic Microscope Systems. To ensure safety, obtain maximum
performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this
manual thoroughly and view the on-line video files before operating the microscope. Retain this instruction manual in
an easily accessible place near the work desk for future reference.
Biomedx Microscope Manual
v12.2014
Page 1
Rule #1 to get the most from your microscope:
READ THE MANUAL
CONTENTS:
Safety Precautions
Getting Ready
Part Names/Overview
Microscope Assembly
Head Assembly
Eyepiece / video
Video connect
HD video connect
Condenser
Light
Completion
Technical Notes
Misc. Application Notes
Zoom Systems
Getting a Specimen on Screen
Problems & Troubleshooting
Misc. Wiring Diagrams
Getting Video to Computers & Displays
Printing Pictures
Going Wireless
Camera Manual for Standard Def. Camera
HD camera notes
Page 3
4
5
6
8
9
10
11
12
13
16
18
26
30
33
35
38
49
50
51
52
58
ON-LINE VIDEO FILES
Find a video review of the microscope as well as set up
and adjustment tips at: biomedx.com/support or
youtube.com/biomedx
Click on this playlist for Biomedx Microscope Info.
Either YouTube or biomedx.com may have updated videos from time to time about the
microscope. Check from time to time if you have an interest or want a refresher on operation.
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Page 2
SAFETY PRECAUTIONS
1. After the equipment has been used in an observation of a specimen that is
accompanied with a potential of infection, clean the parts coming in contact with the
specimen to prevent infection.
 Moving the microscope is accompanied with the risk of dropping the specimen.
Be sure to remove the specimen before moving the microscope.
 In case the specimen is damaged by erroneous operation, promptly take
infection prevention measures.
 The microscope can become unstable if its height is increased by accessories
mounted on it. In this case, take anti-topping measures to prevent the specimen
from being dropped should the microscope topple over.
2. To avoid potential shock hazard and fire, always turn the Fiberoptic Lightsource “off”
and disconnect the power cord from the AC receptacle at the rear of the light house
assembly before replacing the bulb. Allow the bulb to cool before touching it.
3. Install the microscope on a sturdy, level table.
4. The air vents on the underside of the lightsource should never be blocked by placing
the unit on a flexible surface such as a carpet, as this could result in overheating
and cause a fire.
5. Always use a grounded power cord for the lightsource and connect the power cord
correctly and ensure that the grounding terminal of the lightsource power cord and
that of the wall outlet are properly connected.
6. When installing the Fiberoptic Lightsource, route the power cord away from the
underside of the box. Should the power cord come in contact with the hot base, the
power cord could melt and cause electric shock.
7. Never turn the power switch “on” while any metallic object is inserted into the air
vents of the lightsource box as this will result in electrical shock, personal injury and
equipment damage.
8. When the microscope is not in use or when it is malfunctioning, disconnect the
power cord from the lightsource, the video camera, monitor and computer (if
connected) from the AC receptacle or from the wall outlet. There is no “on/off”
switch for the video camera so disconnecting the camera’s power supply main AC
power cord from the AC receptacle or wall outlet is how it is turned “off”.
9. Do not look directly into the lightsource fiberoptic porthole while the unit is “on” . The
light intensity can be painfully bright if you directly look at the light. Likewise, always
look into the microscope objectives with light intensity lowered and bring light up to
satisfactory level for the specimen being viewed.
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GETTING READY
A microscope is a precision instrument. Handle it with care and avoid subjecting it to
sudden or severe impact.
Do not use the microscope where it is subjected to direct sunlight, high temperature and
humidity, dust or vibrations.
The tension of the coarse focus adjustment knob should only be adjusted by means of
the tension adjustment ring.
Heat from the 150 watt quartz halogen lightsource is led away by a fan directed out
from under the unit and also through natural convection. Consequently, leave an
enough space under the lightsource for heat to escape and don’t block the front or rear
of the unit. Ensure that the room is well ventilated.
BIOMEDX
FIBEROPTIC
When moving the microscope, carefully
carry it with one hand under the base 1
and the other hand holding at the
recessed handle on the rear of the arm
2 as shown in the illustration on the left.
Take care to not squish or kink the fiber
optic cable when moving or placing into
a travel case.
*Damage to the microscope will occur if you hold it by the stage, X-axis/Yaxis knob, binocular section of the observation tube, etc. Also make sure
that eyepieces, specimen, filters, etc. do not fall off.
*Sliding the microscope on the surface of the table may damage or tear off
the rubber feet and/or scratch the table top surface.
Maintenance and Storage
Clean all glass components by wiping gently with gauze or lens paper slightly
moistened with something like Sparkle glass cleaner (www.glasscleaner.com) and do
the same to remove fingerprints or oil smudges.
Do not attempt to use organic solvents to clean the non-optical components of the
microscope. You can use Sparkle glass cleaner or a neutral detergent.
Do not disassemble any part of the microscope as malfunction or damage may occur.
When storing the microscope, put it in a locker or case or keep a dust cover on it.
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PART NAMES/OVERVIEW
BIOMEDX
FIBEROPTIC
* When the microscope ships the stage has two transport pins to lock the stage in place and
those must be removed prior to operation. Pull up vertically on the rubber pin to release it.
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MICROSCOPE ASSEMBLY
When you get your microscope you will find that the various parts are packed in different
boxes. You will find it advantageous to take everything out of the boxes and lay them on
a clean work surface so you can then begin the identification process and assembly.
You may be very excited to get your new microscope up and running and if you are
mechanically inclined, you may find yourself jumping right into the parts and pieces of
the scope and begin to assemble. It will be advantageous to read these instructions
before beginning. You should also review the online video files as you will refer to the
techniques on the video files to gain proper microscope adjustment tips.
Video Files online:
1.
2.
3.
4.
5.
6.
Microscope Review
Turret Condenser Set-up
Adjusting a Phase Contrast Condenser
Adjusting a Darkfield Condenser
Using an Oil Objective
Getting a Blood Sample
Access at www.youtube.com/biomedx or www.biomedx.com/support
Depending on your chosen configuration, you may have parts and pieces that differ
somewhat from what is shown in this manual. You may have some components and
not others though in all respects the basic nature of the assembly of parts does not
differ.
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When you lay everything out on your workspace you will see something like the
pictures below differing only in the items that were selected in your particular
configuration. You will note the absence of separate microscope objectives as
these have already been mounted on the microscope’s nosepiece turret. Biomedx
pre-assembles, pre-checks and pre-adjusts everything prior to shipping to you and
we simply leave the objectives in place.
Video camera pre
-attached to 1x
video microscope
coupler.1
Camera
AC/DC
power supply &
video hook-up
cables.
Microscope base frame w/
integrated stage and fiberoptic
light guide.
150 watt quartz
halogen lightsource.2
AC power cord
for lightsource.
Trinocular head assembly.
Hex wrench.
Condenser set-screw extender tool.
Standard
eyepieces.
Turret Condenser.
Field expanding /
centering lens.
Blue Filter.
Video camera pre
-attached to
optical zoom
microscope
coupler.1
Trinocular port
assembly.
Binocular head assembly.
Deluxe rubber
eyecup eyepieces.
1 Your
camera may appear different, also your zoom may appear longer/taller depending upon model, or you might have
a video coupler with the Biomedx optical assembly and HD camcorder.
2 There are multiple styles of the Fiberoptic Lightsource, shown here is one version.
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The first thing you will want to do is mount
the eyepiece head assembly onto the base
frame.
Screw
w/hex
head.
You will need to loosen the hex screw on
the top side of the frame with the hex wrench
and then seat the head into proper position.
Examine the bottom of the head and the top
of the frame for an idea of how it fits by just
sliding in from one side and then setting it
down into place. Tighten the screw hand
tight but not so tight as to strip the threads.
Shown to the left is the trinocular head
assembly.
You may have a binocular head assembly and
a trinocular port. In this case you must mount
the trinocular port first.
When the trinocular port is installed you can
then mount the binocular head.
Screw
w/hex
head.
Biomedx Microscope Manual
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Note that there is another hex screw in front
of the trinocular port assembly and this must
be loosened with the hex wrench and then
the binocular head can be seated. When
seated, hand tighten the set screw with the
wrench to hold the head securely in place.
Page 8
There are covers on the eyepiece
lens shafts which must be removed
and then the eyepiece oculars can
be slipped into place.
Whatever video camera you may be using gets screwed onto the video coupler
appropriate for that camera and this assembly gets attached to the microscope in the
same manner as the head assemblies were attached. There is a hex screw which
must be loosened and then the video coupler with camera can be seated in place.
Note that in most cases, any video coupler and camera combination has typically
been preassembled and set up prior to shipping. It is best to always leave the camera
and coupler arrangement as one assembly if removing & transporting system.
Hex screw
on binocular
head.
Hex screw on
trinocular port.
Hex screw that holds
head assembly onto
base of microscope.
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Page 9
If you have a standard definition video camera (as shown here), the top of the video
camera has the receptacles which must receive the appropriate jacks from the 3wire end of the wire harness which is part of the universal 110-220VAC to 12V DC
power supply. These three receptacles are labeled VIDEO, S-VIDEO and 12VDC.
There is no connection to the 4 pin square receptacle labeled LENS.
VIDEO is a standard composite video output . The output jack on the camera seen
here is a BNC female connector. The wire in the wire harness has an RCA style
male connecter with an RCA female to BNC male adapter. (RCA style jacks are
also referred to as phono plugs). The VIDEO output is the same type of output you
get from a VCR or camcorder. This output can be directed to the input of an
appropriate RCA/phono plug input jack on a standard TV, a TV/VCR combination
unit, or a TV-PVR (TV-personal video recorder) computer input box.
S-VIDEO is a higher quality output and can be directed to TVs, laboratory monitors,
TV-PVRs, large screen TVs, TV projectors, or any other device that can accept the
signal. This is the preferred output to use and if you are using a standard TV or TV/
VCR you should attempt to locate a unit that can accept this type of input.
PWR When the power jack is plugged in and the AC cord from the power supply on
the other side is plugged into a proper AC outlet, the green LED light labeled PWR
will light.
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If you have a high definition - HD - camcorder you will find the HDMI jack on the
camera (usually mini-HD connector) under an appropriately labeled cover. Plug the
mini-HD connector side of this cable the camera.
Typically the USB connection is used for downloading captured images from the
memory disk in the camcorder to your computer for saving and printing any images
or video that you have taken.
You will need to plug the power cord for the camcorder into the unit as well as to
your AC outlet.
Power plugs in under
flap on this model
here.
HDMI cable here to HDTV in
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Page 11
You will likely have one of the following types of condensers which will need to be
correctly mounted under the stage.
Universal style turret condenser.
Darkfield condenser.
Brightfield condenser.
Raise the stage up by rotating the focus
control knob.
Locate the condenser adjusting knob
and rotate that assembly down.
Lightly loosen the set-screw.
With the condenser positioned as
shown (any type) it will fit underneath
the condenser holder assembly and
slip up into place.
Make sure the condenser is seated all the way up into its holder and then lightly
tighten the set-screw to hold the condenser in place. Use the set-screw extension
tool to reach the set-screw more easily when using the turret condenser.
Proper position for the condenser under the specimen slide is all the way up and
nearly touching the bottom of the slide.
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The Fiberoptic Lightsource FOI-150
This is the on-off switch which
also controls the light intensity.
Power cord plugs in
here in back of unit.
This is the light port that the fiberoptic
cable from the back of the microscope
base plugs into. You may have to loosen
the set-screws first in order to get the
cable plug into the hole. Note the indent
on the plug, this will be where the screws
are tightened down to hold the cable
securely in place.
Fuse drawer.
LAMP REPLACEMENT
The door to access the lamp is under the unit.
Unscrew knob counterclockwise to open (use
screwdriver if needed).
With the lamp access door
open, the lamp is easily
accessible. Pull lamp and
socket directly out of unit.
Hold lamp socket and
gently pull lamp out of the
socket. Replace with new
lamp.
Replace with EKE
lamp only.
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CAUTION: If lamp burns
out when unit is in use, it
can be dangerously HOT!
Let cool before replacing
or wear heat resistant
gloves.
Page 13
The Fiberoptic Lightsource - Desktop
On/Off Button
Light Intensity Control
Set-screw
Bezel
Lamp Change Compartment
Filter Holder.
Plug the fiberoptic cable from
the back of the microscope base
into the lamp house light port.
You may have to loosen the setscrew first in order to get the
cable all the way into the hole.
Lightly tighten the set screw to
hold the fiberoptic cable in place.
Electronic Regulation
The light intensity control knob
must be turned anywhere from
the 9 o’clock to the 1 o’clock
position before you will notice
any output. This is normal and
does not affect performance.
Plug the AC power cord into the back
of the lamp house and the plug into a
properly grounded AC outlet. The
microscope light and intensity is
controlled by the on/off switch and
the light intensity control on the front
of the lightsource unit.
Power rating
Fan
Power
connector
Use of Filters
This lightsource accepts the use of 1” diameter filters. We recommend dichroic filters for long life and fade
resistant performance.
Seldom or intermittently used filters should be installed in the filter holder, with the coated side facing the
lamp. If an IR (infra red) filter is to be used, install the IR filter in the bezel, coated side facing the lamp.
Then install the adapter. You may wish to install a colored filter with the IR filter in the bezel, rather than
use the filter holder accessory. All additional filters installed in the bezel should be installed after the IR
filter.
Some filters may be supplied with a protective plastic film covering the coating. BE SURE TO REMOVE
THE FILM BEFORE USE!
Filter Drawer with Holder.
Install seldom used or
intermittent use filters here.
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Bezel accepts filters too!
Install IR and/or long term
use filters here.
Page 14
Lamp Replacement
Depress the power button to the “Off” position.
(Button Out)
Lamp Release Lever
Lamp
Lamp
Socket
Allow lamp to cool. CAUTION: Lamp, socket,
and surrounding surfaces may be hot!
Turn cover lock knobs counter clockwise until
the lamp module “springs” out from the housing
and extend tray out.
Push the lamp release lever toward the socket
to raise the lamp and remove it from the lamp
socket, pulling the lamp from the socket.
Insert a new lamp into the socket. Make sure
lamp release lever has been returned to its
original position, push the lamp all the way
down into its holder.
Cover Lock Knobs
Be careful not to touch the bulb or the inside of the
reflector. Finger prints may affect the light output.
Fuse Replacement
Under normal use, the fuse should not require
replacement. The purpose of the fuse is to protect the
electronics from failure due to inrush of AC current. should
it become necessary to change the fuse, replace with the
same type (120/100 V: 2.5 amp/slow blow. 230/240 V” 1.6
amp/slow blow) to insure long life and performance.
Use EKE (preferred) or DDL lamps.
The Fiberoptic Lightsource can accept an EKE labeled lamp as standard. The EKE is a high intensity lamp rated
at 200 hours life expectancy when run at maximum intensity. Since this is often not the case, it typically lasts
much longer. The DDL lamp is the other option. This lamp is rated at 500 hours life if run continually at maximum
intensity but it is not as bright as the EKE. For all of the work with live blood viewing using phase contrast and
darkfield, the EKE lamp is preferred. For mostly brightfield or phase contrast applications, the DDL will be okay.
Both lamps are very bright and will generally continue to operate well past their rated life expectancy when used in
normal day to day operation and not at maximum brightness.
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Fiberoptic Lightsource
Fiberoptics Technology unit w/rear
lamp saver switch.
Lumina Series.
Plug the fiberoptic cable from
the back of the microscope
base into the lamp house.
You may have to loosen the
set-screw first in order to get
the cable all the way into the
Lightly tighten the set screw
holding the cable in place.
Plug the AC power cord into
the back of the lamp house
and the plug into a properly
grounded AC outlet. The
microscope light and intensity
is controlled by the on/off
switch on the front of the
CHANGING THE LAMP - The time will come when the lamp will burn out. If you change the lamp in the middle
of a session when the scope is being used, the lamp can be very hot. Use caution.
Loosen the screw on top
of the lightsource.
The front door will drop
down.
Raise the lamp
extracting arm to
bring the lamp out
of its retaining clip.
Lightsource Fuse Replacement
The fuse in the 120v N. American unit is located in a pull-out drawer
incorporated into the power cord receptacle. There are two
compartments. The fuse goes into the forward compartment, the back
one is for storing a replacement. Replace with 5x20 3.15 Amp Fast
Acting Fuse. The 220v model has a circuit breaker.
The Fiberoptic Lightsource can accept an EJA or EKE labeled lamp.
The EJA is a high intensity lamp rated at 40 hours life expectancy when
run at maximum intensity. This is the preferred lamp for oil darkfield
application. The EKE lamp is the other option. This lamp is rated at 200
hours life if run continually at maximum intensity. For all of the work with
live blood viewing using phase contrast and occasional darkfield, the
EKE lamp is fine. For mostly darkfield applications, you can stick with
EJA lamps. Both lamps are very bright and will continue to operate well
past their rated life expectancy if they are not operated continually at
constant brightness.
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Remove the lamp from
the socket and replace.
Lower the extracting
arm back in place and
then gently push the
new lamp into position
under the retaining clip.
Use EJA or EKE lamps.
The black Fiberoptics
Technology unit comes
with a rear lamp saver
switch. This will extend
the life of the installed
lamp when operated at
full intensity as it
decreases the power
going to lamp
(decreases lumen
output as well).
Page 16
Following the assembly procedure
your microscope should look very
close to one of the two units
shown here.
Hook your camera up to a monitor, plug
in the power and you’re ready to go!
The standard definition video camera can be plugged into a monitor that has
a composite or s-video input (the s-video is preferred). An HD camcorder
should be plugged into an HDTV display via the HDMI connection.
If you have not yet done so, now is the time to view the CD-Rom video files
which will discuss the finer points of set-up, adjustment and operation.
The pages to follow cover some more technical points, miscellaneous
application notes, adjusting your camera and monitor for the best view,
illustrations of some sample wiring diagrams, and getting your video image to
a computer or computer monitor.
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TECHNICAL NOTES
Field Iris Diaphragm
For bright field applications, using the field iris
diaphragm ring 1 , adjust the diameter of the
field iris for objective power to the extent that it
just circumscribes the field of view. When the
field iris diaphragm is stopped down to
circumscribe the field of view, it will exclude
extraneous light and improve image contrast
within the field of view.
*When using the 100X objective, the field iris
diaphragm image will not be visible within
the field of view. Accordingly, stop down the
diaphragm to its smallest diameter.
Slider
The slider is used for filter sets. Typically a blue
filter can be used for low light brightfield
applications where a low light level will produce
a more yellow light. The blue filter will bring the
eyepiece view back to daylight color. The video
cameras will self adjust for any color
temperature.
The slider is also used to accommodate an
optional transmitted light analyzer. By preparing
a transmitted light polarizer and polarizing light
condenser, simple polarized light observation
becomes possible for gout testing and crystal
urinalysis.
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Adjusting
Tension
the
Coarse
Adjustment
Knob
The coarse adjustment knob tension is preadjusted for easy use. However, if desired, one
can change the tension using the tension
adjustment ring 1 . Applying a large flat-bladed
screwdriver to any of the grooves 2 on the
circumference of the ring, turning the ring
clockwise (in the direction of the arrow)
increases tension, and vice versa.
The tension is too low if the stage drops by itself
of focus is quickly lost after adjustment with the
fine adjustment knob 3 . In this case, turn the
ring in the direction of the arrow to increase
tension.
Pre-focusing Lever
The pre-focusing lever ensures that the
objective does not come in contact with the
specimen and simplifies focusing.
After focusing on the specimen with the coarse
adjustment knob, turn this lever 1 clockwise (in
the direction of the arrow) and lock; the upper
limit on coarse adjustment movement is set at
the locked position.
Focusing using the fine adjustment knob is not
affected by the pre-focusing dial. Accordingly,
after using the coarse adjustment knob to lower
the stage for changing specimens or applying
immersion oil, refocusing is easily accomplished
by rotating the coarse adjustment to reach the
pre-focusing position, then making fine
adjustments with the fine adjustment knob.
*When not required, leave the pre-focusing
dial unlocked.
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Placing the Specimen
*Releasing the curved finger with great force
or suddenly releasing your grip on the
curved finger knob 1 while releasing the
curved finger will crack or damage the slide
glass. Always place the specimen with great
care.
Observation with Specimen Holder for
Single Specimen Slide
I.
Turn the coarse adjustment knob
2
counterclockwise (in the direction of the arrow)
to lower the stage.
2. Open the spring-loaded curved finger 3 on
the specimen holder and place the specimen
slide into the specimen holder from the front.
3. After placing the slide as far as it will go,
gently release the curved finger 3 .
Observation with Specimen Holder for Two
Specimen Slides
1. Place the first specimen slide as described
in steps 1 and 2 above, then place the second
specimen slide so that it contacts the first
specimen slide.
2. Gently release the curved finger 3 .
Observation by Placing the Specimen Slide
with One Hand
Place the specimen slide at the front of the
stage, then slide the specimen slide on the
stage surface to slowly and gradually open the
curved finger in the direction of the arrow. Insert
the specimen slide into the specimen holder
until it is fully and properly seated in the
specimen holder.
Cover Glass
Use cover glasses of 0.17 mm thickness (#1) in order to allow the objectives to exhibit their full performances.
Specimen Slide
Use specimen slides of 0.9 to 1.4 mm thickness. Using thicker specimen slides may result in inaccurate imaging of the field
iris diaphragm image on the specimen.
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Moving the Specimen
Turn the upper knob which is the Y-axis knob 1
to move the specimen in the vertical direction,
and turn the lower knob which is the X-axis knob
2 to move it in the horizontal direction.
*Do not use the specimen holder or stage to
move the specimen for this will damage the
rotating mechanisms of the knobs.
*When the stage and specimen holder are
stopped by the prefocusing dial, the tension
of the X-axis/Y-axis knobs increases. In this
case, do not attempt to turn the knobs to
move the stage beyond the stopped position.
Adjusting the Interpupillar Distance
While looking through the eyepieces, adjust for
binocular vision until the left and right fields of
view coincide completely. The index dot indicates
the interpupillary distance.
Note your interpupillary distance so that it can be
quickly duplicated.
Adjusting the Diopter
1. Looking through the right eyepiece with your
right eye, rotate the coarse and fine adjustment
knobs to bring the specimen into focus.
2. Looking through the left eyepiece with your
left eye, turn the diopter adjustment ring 1 to
focus on the specimen.
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Using the Eye Shades (Deluxe Eyepiece)
When Wearing Eyeglasses
Use with the eye shades in the normal, foldeddown position. This will prevent the eyeglasses
from contacting and scratching the eyepieces..
When Not Wearing Eyeglasses
Extend the folded eye shades in the direction of
the arrow for efficient use of the eyeshades by
preventing extraneous light from entering
between the eyepieces and eyes.
Using the
(Optional)
Eyepiece
Micrometer
Disk
Prepare one eyepiece micrometer disk
(diameter 20.4 mm, thickness 1 mm) and two
20.4-RH reticle holders (available as 2-piece
set). The field number becomes 19.6 when the
reticle holders are used.
If your eye is poor in eyesight and cannot focus
the micrometer, use eyeglasses to correct your
vision.
1. Remove both eyepieces.
2. Hold the micrometer disk 1 with the side
with indications facing down and place it into a
reticle holder 2 .
*Be careful not to leave dirt on the
micrometer disk, as it will be noticeable
during observation.
3. Screw the reticle holder 2 containing the
micrometer disk into the bottom of an eyepiece.
Be sure to screw in all the way by hooking your
nail into the notch 3 on the holder at the end.
4. Screw the other reticle holder, alone, into the
other eyepiece in order to align the field number.
5. Attach the eyepieces again.
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Centering the Field Iris Diaphragm (Brightfield apps)
I. With the 10X objective engaged and the specimen
brought into focus, turn the field iris diaphragm ring 1
counterclockwise to stop down the diaphragm to near its
minimum size.
2. Turn the condenser height adjustment knob 2 to
bring the field iris diaphragm image into focus.
3. Rotate the two auxiliary lens (field expanding lens)
centering knobs 3 to adjust so that the field iris
diaphragm image is centered in the eyepiece field of view.
(Figs. 1 & 2)
4. To check centration, open the field iris diaphragm until
its image touches the perimeter of the field of view. If the
image is not precisely inscribed in the field of view, center
again. (Fig. 2)
5. When used for actual observation, open the field iris
diaphragm until its image is slightly larger than the field of
view.
Fig. 1
Aperture Iris Diaphragm
 The aperture iris diaphragm determines the numerical
aperture of the illumination system. Matching the
numerical aperture 1 of the illumination system with that
of the objective provides better image resolution and
contrast, and also increases the depth of focus.
 Since the contrast of microscope specimens is
ordinarily low, setting the condenser aperture iris
diaphragm to between 70% and 80% of the NA of the
objective in use is usually recommended. If necessary,
adjust the ratio by removing the eyepieces and looking
into the eyepiece sleeves while adjusting the aperture iris
diaphragm knob 2 until the image shown in Fig. 3 is
seen. (Fig. 4)
*lf the aperture iris diaphragm is set too small, image
ghost may be observed.
Fig. 2
Fig. 3
Using the numerical aperture scale
Align the aperture iris diaphragm knob 2 with the NA
value 1 on the scale. The scale value should correspond
to the NA number engraved on the objective.
Since the aperture iris diaphragm knob has a certain
width, align the center line engraved on the knob with the
scale indication.
When using a 100X objective, turn the aperture iris
diaphragm knob 2 all the way to 0.9 on the scale. (Fig. 4)
Fig. 4
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Using Filters
You can place a filter in the light path with
either method:
Insert a filter with diameter of 32.5 mm 1 in a
brightfield condenser with a filter holder
inserted below the condenser.
Insert one or more filters with a diameter of 45
mm 2 on the light exit glass on the
microscope base.
Common filters:
Polarizing filters,
Neutral density filters,
For some applications with phase contrast, a
green filter resulting in a green wavelength of
light will resolve to a higher degree.
Using the Low Magnification
Field Expanding/Centering Lens
This lens was reviewed on the prior page for
centering but it is also designed for providing
illumination
which
covers
the
entire
illumination field when using a 2X objective.
This lens is normally pressed into place below
a specified condenser, like the turret or
brightfield condenser.
This lens is designed exclusively for use in
observation.
As
the
aperture
iris
diaphragm cannot be seen and field iris
diaphragm cannot be used when this lens
is used, set both iris diaphragms to the
fully open conditions.
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Using Oil Immersion Objectives
*Be sure to use the provided Olympus immersion
oil.
1. Focus on the specimen by switching the objectives
from the lowest power to highest power.
2. Before engaging the immersion objective in the
light path, place a drop of immersion oil onto the
specimen cover slip at the area to be observed.
3. Turn the revolving nosepiece to engage the
immersion objective, then focus using the fine
adjustment knob.
*Since air bubbles in the oil will affect the image
quality, make sure that the oil is free of bubbles.
a. To check for bubbles, remove the eyepieces and
fully open the field and aperture iris diaphragms, then
look at the exit pupil of the objective inside the
observation tube. (The pupil should appear round and
bright)
b. To remove bubbles, turn the revolving nosepiece
to move the oil immersion objective back and forth a
few times.
*lf the condenser engraving shows a numerical
aperture (NA) of 1.0 or higher, the number applies
only when oil is applied between the slide glass and
the top surface of the condenser. When oil is not
present, the NA is about 0.9.
4. After use, remove oil from the objective front lens
by wiping with gauze slightly moistened with an ether
(70%)/alcohol (30%) mixture, or Sparkle glass
cleaner.
(If you use ether/alcohol mix, do not use on painted
surfaces/labels, use caution as it is highly flammable,
use in well ventilated room.)
Caution in use of the immersion oil
If immersion oil penetrates in your eye or attaches to your skin, apply the following
treatment immediately.
Eye: Rinse with fresh water (for more than 15 minutes)
Skin: Wash with water and soap.
When the appearance of your eye or skin is altered or pain continues, immediately
consult the doctor.
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MISC. APPLICATION NOTES
Your Biomedx Fiberoptic unit is an Olympus CX41 microscope that has been
modified and upgraded with a fiber optic light guide, an external high powered
light source (like a 150 watt quartz halogen unit), and a special brass focus gear
which offers more rugged travel options over the stock plastic that was prone to
break during travel excursions.
The microscope employs a UIS (Universal Infinity System) optical design, and
should be used only with the UIS eyepieces, objectives and condensers, etc. Less
optimum performance may result if inappropriate accessories are used.
TURRET CONDENSER: The turret must be rotated into proper position to match
your objective and desired mode of operation. The settings are “O” for brightfield,
“D” for darkfield, “ph1” for 10x & 20x phase (not used unless you have a 10x or
20x phase objective), “PH2” for 40x phase, and “PH3” for 60x & 100x phase.
Make sure when installing the condenser assembly that you seat the condenser
ALL THE WAY UP into its holder and then lightly tighten the set screw.
The turret condenser has been pre-adjusted for phase contrast using the
objectives sent with your system. Once set, it typically never changes. See the CD
-Rom video files for adjustment specifics.
DARKFIELD CONDENSER: If you have a darkfield condenser, this condenser
requires that optical immersion oil be used to get any image. See video files.
FIELD IRIS: A field iris is built into the base of the microscope. This is for Koehler
brightfield illumination. The field iris also comes in handy for properly adjusting
darkfield condensers and when using the turret condenser in darkfield mode.
Because the turret condenser does not do darkfield as efficiently as a dedicated
darkfield condenser, we can use the field iris to shut out some of the incident light
when in the darkfield mode. This will give a richer deep black field. Using oil on
the condenser also helps with the darkfield when using the 40x objective along
with varying the vertical position of the condenser under the specimen.
OIL CAUTION: Please DO NOT use oil on the 40x objective (if you have one) it
is a dry objective. You will need to use oil on the 50x or 60x oil iris or 100x
objectives (if you have them) and on the condenser for 50-100x mode. In phase
contrast the 100x objective is great for research and for presentations (big screen
TVs and to impress your friends and neighbors), while the 40x is great for a clinic
when busy with many clients as no oil cleanup is needed. If you have a dedicated
darkfield condenser and 50x, 60x, or 100x oil iris objectives, these all require oil.
COLOR FILTERS: A blue filter can be used for low light brightfield applications. In
these cases, the microscope turret condenser is set for brightfield mode and the
light often is turned all the way down. This will give a yellow tint to the light and the
blue filter will bring it back to daylight color temperature. Cameras will adjust color
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automatically for the TV monitor in auto mode so it is natural for the color to shift somewhat
as you pan from one end of the slide to the other depending upon your specimen. Some
cameras can be set to manual white balance to adjust for this. We often will pre-set the
cameras when doing the pre-setup check to a white balance color temperature of 3100°
Kelvin. This stops the camera’s auto color setting from continually adjusting and changing
the color slightly as you move around the specimen. With a lock at 3100° K and in phase
contrast mode (with no blue filter), the lightsource would generally be in the 2 to 3 o’clock
position and this will provide good color balance. If you turn the lightsource up past this
point the image on the monitor will just be a bit more blue as the color temperature of the
lamp goes beyond 3100°.
A green filter is often used with phase contrast in some situations. Theoretically, phase
contrast gives a sharper image when a green filter is used. Many individuals prefer to do
microscopic screening without a green filter as they feel the image is more appealing.
However, note that with the extensive control available with the video cameras, you can use
a green filter and tweak the camera settings to bring back just about the same image color
that you may have had without a filter. This will potentially offer slightly higher image
resolution and contrast with the filter and without the green tint in the image. You will have
to experiment with your system if you choose to acquire a green filter.
STANDARD DEFINITION VIDEO CAMERA SETTINGS: (For more camera technical notes
see the camera manual for your camera.) With the advent of HD, we do not often couple
microscopes with standard definition video cameras any longer. If you have such a system,
the standard definition video camera we use for scopes is a medical micro head 1/3” chip
CCD color camera with digital signal processing and the highest resolution available in a
single chip standard TV camera. It has been found to have excellent all around phase
contrast as well as darkfield handling capacity.
You will find that you have an on-screen menu program available by pressing the “set”
button on the camera. From here you can adjust gamma (lightness/darkness), phase
(color), color level, auto adjustments, etc. If you were in phase contrast most of the time the
settings might shift slightly, if in darkfield most of the time the settings may shift in the other
direction. You can experiment with these settings for your particular monitor as you may find
something that better suits your particular viewing monitor. Basically, the settings when sent
from the factory will be close to the following:
Camera Main Menu
Lens: Manual
AGC: 10db
Shutter: Auto
White balance: 3100°
Back light: Off
Text display: Off
Sync mode: Int
Lens must be in “manual”. AGC (automatic gain control) should not be much higher than
10db in most cases as some elements in your viewed image will wash out due to light
intensity (white blood cells for instance when viewing living blood with a dedicated oil
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darkfield condenser). Some people might turn this off which may increase signal to noise
ratio very slightly (and the lens may also be set to video when in darkfield), however you will
need to control the light intensity yourself as the camera will not adjust automatically. If you
go to another mode of the microscope (phase or brightfield). For most this is more hassle
than it is worth.
The shutter should stay in auto mode. White balance should stay in ATW which will track
the white balance to whatever you are looking at or kept at 3100° range if you go back and
forth from viewing two type of specimens in both phase/darkfield and then brightfield (in this
case the “Lens” setting must be set to “manual”). Note that if you are viewing a stained or
dry layer colored slide, the color may shift as you move around as this is the ATW adjusting
for a shift in the image color. The AWC mode will allow you to lock in the white value by
going to a white area on the screen and pressing set to lock it in, however this may alter the
color in phase or darkfield. You can experiment but it might be best to just leave it with a set
color temp of 3100°.
Special Menu
Gamma: 14
Color Adjust: Phase: 0 Level: 0
Sharpness: Low or High (which looks best depends on monitor)
Contrast: Off
Brightness: 0
Flickerless: Off
The camera gamma, phase, level and brightness can be adjusted to suit your personal
preference and monitor strength/weakness for optimum view in phase, darkfield or when
doing a mix of both.
For FLAT PANEL LCD displays, it seems to give a better image when the gamma setting is
turned down.
VARYING IMAGE SIZE TO MONITOR: This is done solely through the optical zoom
assembly if you have one on your scope, otherwise it is a fixed image size variable only
through switching objectives.
MONITOR NOTE: On some TV monitors with video recorders, the signal from the camera
needs to be turned onto the TV screen by way of pressing “input” on the remote control, or
by going down the channel selector from 4 - 3 - 2 -1 and then the line-in will come on, or by
turning the TV off and then on again, or by going to the menu selection on the remote
control and finding the right selection to turn on the line-in which the camera is plugged into.
It is best to read the TV instructions if you are having a problem.
REMEMBER TO ADJUST YOUR SPECIFIC MONITOR’S COLOR, CONTRAST AND
BRIGHTNESS LEVELS FOR OPTIMUM IMAGE QUALITY.
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Sony Laboratory Monitor Guidelines
(Standard Definition Cameras Only)
Sony CRT (Cathode Ray Tube) lab monitors.
These have user adjustable controls WHICH MUST BE ADJUSTED to give you suitable
viewing.
The adjustments may have been made prior to shipping, but if your monitor was drop
shipped from the warehouse, you will need to do this.
Each monitor has its own slight variations in control but the listing below will give you a
good starting point:
Contrast: 85 to 90
Brightness: -2 to 0
Chroma: 66
Phase: 0
Color Temp Should be set to USER < this is important
Driving the red, green and blue guns in the picture tube will give more brightness to the
screen so you can adjust them up to values like:
Gain R 60
G 60
B 40
Bias
R 27
G 27
Tweak these settings up or down to your liking. The Sony CRT Lab monitors have been
discontinued and replaced by Sony professional video flat panel monitors.
Sony flat panel LMD (Lab/Medical Deployment) professional lab monitors.
These are usually good to go right out of the box and give excellent imaging that matches
or exceeds the CRT screens but do have image loss issues when viewing on an angle
due to their flat panel nature. Nevertheless with the discontinuation of the mid-priced CRT
line, the flat panels are the future.
The Sony professional video monitors are designed and optimized specifically for video
and are superior to computer based flat panel displays. About the only thing you might
want to tweak on the monitor is to possibly increase the contrast a bit and maybe adjust
the brightness setting depending upon your room lighting.
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THE OPTICAL ZOOM SYSTEMS
(For Standard Definition Cameras Only)
There are various ways to configure your optical zoom
system. Shown to the right is a 3:1 zoom body. You
might have a 7:1 zoom body which is double the size.
VIDEO CAMERA
Notes for 3:1 short zoom: This zoom basically gives
the ability to have a wider field of view up through what
would be a view using a standard 1x video coupler.
This works well for individuals that are using primarily a
100x oil objective for their viewing, or the darkfield 50x
–100x setup.
However, many individuals desire to use their zoom
with a 40x non-oil or 50 oil objectives and want the
ability to zoom out as well as increase the image size
on the monitor. The standard zoom body with the 2x TV
extender added to the top of the zoom will accomplish
this goal. Consider the 2x TV extender lens optional
and not really necessary with 100x objectives unless
you want very high video magnification.
5mm coupler
2x TV Extender
There is also a 5mm coupler mounted on the camera
which it will require in order to keep the zoom in focus
with the eyepiece oculars as one focuses up and down
throughout the zoom range.
General Zoom Note: With any objective being used,
you are increasing magnification, but not resolution,
therefore as you increase image size, you will also
increase the fuzzy-ness of the image. This is normal.
The advantage that optical zooming has over electronic
digital zooming is the smoothness of the edges. Optical
has a smoother look then digital.
Notes for 7:1 tall zoom: A basic parfocal adjustment
guide is in the rear pocket of this manual. Par focus
means the ability to maintain focus through the zoom
range while also being in focus in the eyepiece.
3:1 ZOOM BODY
Your zoom system was pre-set prior to shipping.
IMPORTANT: The 7:1 zoom is designed for the full
zoom range with low power objectives. It works well
with the 2x objective and the 2x field expanding lens
placed in the condenser.
The effective zoom range using a 40x objective is
about 2.2 to 7 on the zoom barrel dial. When you move
below 2 you will have field aberration going out from
the middle—this is normal as it is beyond the capacity
of the optics for the higher power objectives.
MICROSCOPE
This zoom is the best choice for those individuals that
want a wide field of view at low power with zoom
capability, along with a higher zoom ability using
higher power objectives.
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The 7x optical zoom system is precision set of optical lenses contained in various tube
assemblies. The top most assembly is referred to as a .67x mini tube coupler that
connects the lower zoom optics to the camera. The video camera screws onto this.
You will note that on the complete assembly that there are two silver controls. The top
silver control has the zoom numbers listed—1 through 7. This adjusts your basic zoom
in and out. The bottom silver control knob is for the focus at the maximum (7x) zoom
position. This is factory pre-set. The only time it should be moved is when at 7x
maximum zoom.
Parfocal adjustment means the zoom system will be in focus throughout the zoom
range. This also is pre-adjusted at the factory. For reference this is how it is done:
1. Set the zoom to highest power and move the lower silver adjuster for image focus.
2. Set the zoom to low power. If image is not in focus, loosen the set screw in the Lock
Ring (see diagram below) on the .67x mini tube coupler.
3. The camera focus position is pre-set so any adjustment should be minimal. Turn
the Focusing Ring (while holding the camera to keep from turning) and this moves
the camera up or down to achieve the best focus.
4. Recheck the focus at high power. If okay, the system is parfocal.
5. Tighten the set screw in the lock ring.
Centering the image. This is preset at the factory taking into consideration the various
levels of magnification used and other optic centering concerns. In general, to center
the image, zoom up and down while observing the image on the monitor. Locate a
point or target in the image that does not move off axis during zoom. This is the optical
axis of the image. Adjust the three centering screws (loosen one and tighten another)
to move this target into the center of the monitor. Tighten the centering screws.
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HD CAMCORDER ZOOM
HD Camcorder
Zoom capability using an HD camcorder is contained
within the optics and electronics of the camcorder itself.
When you receive your system set up for a camcorder,
the video coupler attaching to the microscope
(composed of 2 assemblies U-V112 and U-V312) along
with the lens assembly you will be attaching your
camcorder to (U-VCAM) is usually pre-assembled and
need only be attached to the top of the head assembly.
It is onto this you screw your camera if not already
received that way. The Canon HD cameras we typically
recommend have a 37mm to 58mm filter size in front of
the lens. The top of the U-VCAM depending on model
is 37mm or 43mm and an adapter ring may be required
(included with system when camera attached).
The U-VCAM has a very wide angle view when the
camcorder is attached. So wide that when your
camcorder zoom setting is zoomed all the way out, you
will not see much except a round bit of light on your
screen.
37mm to 58mm
adapter if
required.
U-VCAM3758
You will need to zoom in your camera to see an
appropriate image.
Use the remote control for your camcorder to effectively
zoom in and out on your screen.
You may need to get into your cameras menu system
to adjust the zoom ranges setting both optical and
electronic or digital to their maximum capability.
More specifics to camcorders are covered later in the
manual.
U-V312
U-V112
MICROSCOPE
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GETTING A SAMPLE ON SCREEN: Here we will just run through some basic notes
on getting a specimen of something like blood onto the TV monitor.
Use a lancet and poke the finger lightly. You want capillary blood. Let it come up out of
the finger - you may need to gently press just below the lancet prick to start the blood
coming (key word is gently).
Take a cover slip and lightly touch the SMALL drop of blood to the cover slip (do not
touch the glass to the finger as that may smash and damage the blood sample). When
the blood is on the cover slip, quickly place the cover slip onto the microscope slide
which should already be cleaned and setting flat on your work surface or table. If the
blood is thick or dehydrated, you may need to press the cover slip lightly to get the
blood to disperse on the slide. You may LIGHTLY tap or press around the edges of the
blood sample to get the blood to disperse nicely on the slide. GENTLY pressing will not
damage the sample. Place the slide into the slide holder on the microscope stage.
PHASE CONTRAST: Turn the condenser assembly so the condenser is ALL THE
WAY UP under the slide. (It should appear as if the condenser lens is almost touching
the bottom of the slide). Turn the condenser to “ph2” (or “40” if you have an older style
condenser) for using the 40x objective. Turn the 40x objective into place.
Turn the light source to about 2 o’clock on the light intensity dial.
Physically, with your unaided eye, look at your blood sample on the stage and move
the slide around so you can physically see blood clearly being over the light coming up
from the condenser.
Now, if you want the image to appear on the TV monitor while you bring it into focus,
make sure that the prism assembly is pulled out on the side of the head assembly (if
you have one on your system). This will direct light to the camera.
Start to rotate the focus knob and raise the stage. The microscope slide will be about a
finger nail thickness away from the bottom of the 40x objective when you are focused
on blood. As you rotate the focus knob, you will start to see shadows appear which
means you are coming into focus. You may use the fine focus to bring the specimen
into view.
STAGE LOCK: If the stage lock lever on the left focus knob is pulled forward, the
stage may be locked at one level and focusing may not be possible. In this case,
release the stage lock lever by pushing it upright.
DARKFIELD: Put a good size drop or two on the condenser. Put your slide into place
and position it over the condenser in an area where you can physically see the blood
on the slide. Rotate the condenser up to where the oil meets the bottom of the slide
and the drop of oil flattens out a bit under the slide.
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Now you can start with a 10x objective for a very wide field of view, and while looking
into the eyepiece at the blood, bring it into focus. You may need to adjust the
condenser up or down to vary the light intensity. Once you have blood in focus, shift
the 10x out of place, put a drop or two of oil on the cover slip, and rotate the 50x oil iris
objective into the oil, shift it back and forth a bit in the oil to sufficiently set the objective
with oil. Open the iris on the objective, and while looking through the eyepiece, close
the field iris down. This will show you how your darkfield condenser is either centered
or not. With your field iris close down, while looking in the eyepiece the light should be
centered, and if not, turn the silver centering screws on the darkfield condenser to
center the light. Once this is set you can adjust the condenser vertically up and down
to get maximum light output through the condenser. With the light centered and the
field iris still closed down, vertically move the condenser up or down to the point where
you get the brightest and most refined area of light right in the center of your field.
Once you have this, open the field iris and you will have optimally adjusted your
darkfield condenser.
Note that you also have an iris adjustment on your objectives which can further
manipulate the light to give you your desired image. Also note that in darkfield mode,
camera and monitor adjustments can be important factors in getting the best image.
TIP:
If you are using a turret condenser with a non-oil 40x objective in darkfield mode, you
can actually oil the condenser (not the objective) and adjust the vertical height of the
condenser under the slide for better light handling and consequent better darkfield
view.
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PROBLEMS AND TROUBLESHOOTING
If an image does not come up on screen consider the following:
The field iris or condenser iris is closed. Open it up.
The phase contrast or darkfield condenser is dropped down vertically too far under the specimen. Rotate it up
under the slide.
The dedicated darkfield condenser is too far off center. Take it off and look at the underside of the condenser
while you physically move the centering screws and eyeball it to center. Put it back on and go through the
adjustment routine.
The objective lens turret is not set in place.
The objective iris is closed.
The prism knob on the side of the trinocular head is not pulled out.
The camera power is not plugged in.
The camera video output is not connected to the monitor video input.
The monitor video/line input button or menu selection is not set properly.
Phase contrast appears weak, three dimensional, or faded:
First check to make sure the turret condenser is “set” into the appropriate indent.
Adjust the phase ring / annulus to center it with the objective.
Raise the condenser so the top condenser glass appears as if it is touching the bottom of the slide. If not and
it is raised up as far as it will go, make sure the condenser is seated all the way “up” in its holder.
Darkfield image is not available when turning the turret condenser to DF:
Raise the condenser so the top condenser glass appears as if it is touching the bottom of the slide. If not and
it is raised up as far as it will go, make sure the condenser is seated all the way “up” in its holder.
Fuzzy dots or dust particles appear in TV image:
Dust is a bugger to get off—and this is an understatement. In many cases dust particles may show up faintly
and sometimes this will change as the zoom is changed. If the dust is not blaring and a complete distraction,
consider this a normal consequence considering all of the optical elements in the zoom path and the level of
adjustment and magnification we are desiring with our systems.
Biomedx returns to the manufacturers many video cameras and zoom systems which we consider
unacceptable. Within the video camera industry, so many “dead” pixels are allowed to appear on any given
video image to meet acceptable levels. We find this too lenient and are more stringent, and further, we apply
the same level of consideration to dust oriented artifacts on video optics. This generally is not a consideration
in darkfield mode but with zoom systems in phase contrast it is a different ballgame. Basically the center 30%
of an image should be clean, with only a few dust “spots” off-center acceptable.
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If you should take your standard definition camera off of your zoom system or take your lenses apart and
then experience particles that were not there in the past, you can attempt to clean this off. Generally any dust
showing up on the image will be dust on the glass covering the CCD chip of the video camera or on the zoom
module top lens. To know which it is, loosen the zoom coupler and the camera while you have an image on
the monitor, then while holding the camera still, rotate the zoom coupler and watch the video image. If the
dust rotates, the dust is on the zoom lens, usually closest to the CCD chip. If the dust particle does not move,
the dust is on the CCD chip cover glass.
First, attempt to blow off the offending particle with an air can. (NOTE: NEVER place the air nozzle on a lens
and then pull the trigger as propellant can be discharge and coat the lens which will be a bigger problem.
Lightly pull the air trigger first and then move the nozzle to the lens.) In darkfield mode you will never notice
dust in the video path, but this is not so with phase contrast because of the gray background.
BIG NOTE: wiping any lens in the video path on a high powered microscope is fraught with peril. It is VERY
EASY to make a small problem worse, and it can take quite a while of playing around, wiping the lens,
blowing with air, vacuuming and such to try and get spots out of the final image. With that said, you can
attempt to get particles of dust cleaned up by wiping the offending lens and CCD cover lightly with lint free
lens tissue. If that did not work, put one or two drop of lens cleaning solution (from a camera store or you can
try basic glass cleaner like “Sparkle” glass gleaner which is very good) on your tissue and clean lens.
Humidity helps to keep down static electricity which can seem to charge the lens and attract dust.
With HD camcorders, we have found in our testing that the Canon cameras had basically clean CCD or
CMOS chips over others we tried, so we recommend these in particular.
If any spot might show up on the screen, it might be when there is no specimen being viewed and the camera
is somewhat focusing on a bit of something somewhere in the optical path, usually the lens nearest or on the
camcorder itself. When you focus on a specimen slide, the spot disappears as it no longer is in the focus
field.
FIBEROPTIC LIGHTSOURCE
If you are unsuccessful at resolving the following conditions, contact Biomedx for a Return Material
Authorization (RMA). Do not attempt to repair the light source. Tampering with the electronics will void the
warranty.
Fan operates, but low light output.
Check the lamp to ensure that it is fully seated. See “Installing the lamp" section.
Check to make sure the fiber optic component is fully seated.
Check the lamp. A partial short in the lamp may cause low or intermittent output.
Check the intensity setting.
Fan operates, but output is intermittent (every few minutes, lamp turns off and turns on).
The light source is running too hot. A thermal cutoff protects the circuitry from heat failure. Check air intakes
and exhaust areas for dust or dirt accumulation. Make sure the unit is installed on a flat hard surface and
there is a minimum of 4 inches space behind the unit’s fan. If not, move the light source to another location.
NEVER enclose the light source without adequate ventilation.
Fan operates, but the light turns briefly on and then off.
1. The lamp may have a faulty filament. Turn the intensity control knob to maximum setting to test the lamp.
2. If the lamp fails, change the lamp following the instructions in this manual.
3. Examine the lamp socket assembly for damage and continuity.
Fan does not operate, light is dim or non-existent.
Make sure to use the correct model for the power available. Running a 240V unit in
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120/100 volt conditions will stop the fan and dim the lamp.
Fan operates, light is not on.
Replace the lamp.
Fan does not operate, light is on.
Return to Biomedx.
Fan and lamp are not working.
Make sure the power cord is inserted completely into the IEC connector and also into the
correct power source. Check the power cord for damage. Check the fuse.
Fiber input is burning.
This could occur if you were using the Fiberoptic Lightsource with another mfg cable. Check the fiber
type. It may be plastic and susceptible to burning, even with the use of a standard IR heat filter. All
Biomedx fiber optic light guides are made with glass fibers.
Ask your supplier about the epoxy used to manufacture the fiber optic input. Some epoxy types cannot
withstand the high temperatures developed in the light source.
Check the lamp type. Replace the lamp with Biomedx approved types EKE or EJA. (Use an IR filter if
possible. Fiberoptic inputs damaged by use of non-approved lamp will void the fiber warranty.
Lumina Series Fiberoptic LIghtsource Power Supply Specifications
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The wiring diagrams that follow show various ways to hook up the microscope to various video
monitors, computers, and printers. Many variations exist depending on your particular
equipment and needs. These are here to give you basic guidelines and ideas.
Microscope Cable Hook-up with Basic TV/VCR
- Standard Definition Camera (or when using standard video out on HD camcorder)
Video Out
Power
Top of Camera
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
Video In Audio In
120VAC
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Microscope Cable Hook-up with Computer Monitor
- Standard Definition Camera (or when using standard video out on HD camcorder)
Power
Y/C (s-video)
Top of Camera
VGA Etc. In
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
VGA Etc. Out
Interface box like:
RTV - VEG or SIIG
Y/C (s-video) in
120VAC
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Microscope Cable Hook-up with Laptop / Printer
- Standard Definition Camera (or when using standard video out on HD camcorder)
Y/C (s-video)
Power
Top of Camera
Computer Printer
USB
Laptop / computer
USB
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
USB
TV-PVR Interface box:
K-Stream, SIIG, Etc. Y/C (s-video) in
120VAC
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Microscope Cable Hook-up with Lab Monitor & Laptop / Printer—Example 1
- Standard Definition Camera (or when using standard video out on HD camcorder)
Video
Power
Y/C (s-video) Out
Top of Camera
A
B
Menu
\/ /\
Exit Power
Back of Monitor
Line A
Y/C In Video In Audio In
Out
Out
Out
Line B
Video In Audio In
Y/C In
Out
TV-PVR
S-Video In
USB
Computer / Laptop
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
USB
Printer
USB
120VAC
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Microscope
Cable Hook-up
- Standard Definition Camera (or when using standard video out on HD camcorder)
Video
Out
Power
Y/C (s-video) Out
Top of Camera
A
B
Menu
\/ /\
Exit Power
Back of Monitor
Line A
Y/C In Video In Audio In
Out
Out
Out
Line B
Video In Audio In
Y/C In
Out
TV-PVR
Composite
Video In
USB
Computer / Laptop
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
USB
Printer
USB
120VAC
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Microscope
Cable Hook- Standard Definition Camera (or when using standard video out on HD camcorder)
Video
Out
Power
Y/C (s-video) Out
Top of Camera
A
B
Menu
\/ /\
Exit Power
Back of Monitor
Line A
Y/C In Video In Audio In
Out
Out
Out
Line B
Video In Audio In
Y/C In
Out
VCR
Video In
Video Out
TV-PVR
S-Video In
USB
Computer / Laptop
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
USB
Printer
USB
120VAC
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Microscope
Cable Hook-up
- Standard Definition Camera (or when using standard video out on HD camcorder)
Video Out
Power
Top of Camera
Video Printer
Video In
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
Video Out
Video In Audio In
120VAC
If you had a TV set with no VCR and wanted to hook up a video recorder to the above set-up, put it some where in the line between the
camera Video Out and the monitor Video In. For example, go camera video out to recorder video in, recorder video out to printer video in,
printer video out to monitor video in.
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Microscope
Cable Hook- Standard Definition Camera (or when using standard video out on HD camcorder)
Video Out
Power
Y/C (s-video)
Top of Camera
Video Printer/Computer Capture
Video Out
Video In
Line A
Y/C In Video In Audio In
Out
Out
Line B
Video In Audio In
Out
Out
Out
Back of Monitor
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
A
B
Menu
\/ /\
Exit Power
120VAC
When you have the video printer and lab monitor, hook it up as shown above. Your standard viewing will be on Line A with the higher quality
Y/C input. When you want to view the screen menu for the video printer, push the button for Line B which will switch you to view what is
occurring with the printer. When generating a print from the printer, you can switch back to Line A and continue to scan with the scope.
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Microscope
Cable Hook-up
- Standard Definition Camera (or when using standard video out on HD camcorder)
Video Out
Power
VCR—Video Cassette Recorder
Y/C (s-video) out
Video Out
Video In
Top of Camera
Video Printer/Computer Capture
Video Out
Video In
Line A
Y/C In Video In Audio In
Out
Out
Line B
Video In Audio In
Out
Out
Out
Back of Monitor
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
A
B
Menu
\/ /\
Exit Power
120VAC
When you have a video printer, VCR and lab monitor, hook it up as shown above. Your standard viewing will be on Line A with the higher
quality Y/C input. When you want to view the screen menu for the video printer or view what is on the VCR, push the button for Line B. When
generating a print from the printer, you can switch back to Line A and continue to scan with the scope. The VCR and video printer will both
need to be turned on to view them on the monitor. The VCR will also have to be turned on in order for any video signal to get to the printer. It
is important that you have the VCR set on the correct input/output arrangement via your VCR remote control or menu selection to have the
signal from the camera get through the VCR to the printer and monitor. If you get a microscope picture on your monitor on line A, and you
don’t have an image on line B, check that VCR and printer are turned on and the correct menu selection is set for both units. See the owners
manual on those units for assistance.
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Microscope
Cable Hook- Standard Definition Camera (or when using standard video out on HD camcorder)
Video
Out
Power
Y/C (s-video) Out
Top of Camera
A
B
Menu
\/ /\
Exit Power
Back of Monitor
Line A
Y/C In Video In Audio In
Out
Out
Out
Line B
Video In Audio In
Y/C In
Out
Video Printer
S-Video In
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
S-Video Out
120VAC
When you have video printer with s-video capability and a lab monitor, hook it up as shown above. Your standard viewing will be on Line A
direct from the camera. When you want to view the screen menu for the video printer, push the button for Line B which will switch you to view
what is occurring with the printer. When generating a print from the printer, you can switch back to Line A and continue to scan with the scope.
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Microscope Cable Hook-up with HD Camcorder, Screen, Computer, Printer
- High Definition Camcorder USB
Out
Power
HDMI Mini-HD out
HD-TV Flat Panel Display
Back of Monitor
HDMI In Video In Audio In
Out
Out
Computer / Laptop
USB
Printer
Fiberoptic cable from back
of scope into 150w
lightsource
12v Pwr Supply
USB
120VAC
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GETTING YOUR VIDEO CAMERA IMAGE
TO A COMPUTER
For watching your microscope image on a computer monitor with a standard definition
video camera while going THROUGH a computer or for connection to a laptop, the easiest
way to accomplish this is to use an external USB TV Tuner . These are small boxes or
USB dongles that allow you to watch broadcast TV on your computer—but instead of
watching television we will watch the microscope camera.
There are many of these devices on the market. The important thing when you go in
search of a unit is to make sure that the specifications state that there is s-video input or
composite input capability. We will be going from the s-video output of the microscope
standard definition camera to the s-video input of the device.
If using a HD camcorder, you would need the proper AV connection wire to plug into your
camera’s side panel AV jack which has a composite video jack (yellow in color) and this
would be plugged into the TV Tuner USB device. (For most people using HD camcorders,
the primary view screen would be a HDTV and all video/image capture is done on the
internal SD memory card. More about that on the next two pages.)
To see a selection of TV Tuner USB units available, go to any internet electronics provider
and type in the search words “TV-PVR” “PC TV Tuner”, “TV Tuner USB” etc. Search
electronic retailers like tigerdirect.com (tigerdirect.ca in Canada), newegg.com, etc.
The latest one we have used which worked for some laptops was the Pinnacle PCTV HD
Ultimate stick. The software to run the device is on a chip inside the unit and when the
device is plugged into the USB port on a computer the software automatically loads and an
icon appears on the screen. Double clicking on the icon brings up the program, then going
to channel selection and selecting the s-video input (with the camera s-video output
plugged into the unit) the microscope image comes up on screen. K-World, Aver and
others have also worked.
For PAL cameras (used in many Euro countries and other areas) the above systems
usually can be switched to that format. Most of these systems are for Microsoft Windows
based computers, you will have to look for ones specific to Apple systems.
For APPLE computers check:
http://www.elgato.com/
Not sure of the video connections to the following, you'd have to check.
http://www.welovemacs.com/vicacrforg4m.html
http://www.welovemacs.com/6201eytv.html
http://www.welovemacs.com/6211eyt2.html
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LARGE SCREEN DISPLAYS with standard definition cameras
If you want to go to a large panel display, plasma as opposed to LCD would be a first
choice due to wide viewing angle, fast refresh rate and deep blacks. Because many
large panel displays now only come in HD format and we are going from a standard
definition signal to a high definition display the image may suffer depending on the
internal scaling ability of the monitor. Some have ok scalability, some very poor. First
you would like to use a 720p monitor. If using one of these or a 1080i or 1080p and
the image looks fractal like, you could use an external video scaler. This is placed
between the microscope camera and the high def display. (The s-video output from
the camera goes into the scaler, and the HDMI output of the scaler goes to the large
panel/plasma display). By referring to the menu of the scaler and selecting the
maximum fine tuning adjustments for standard definition inputs, you will get the most
optimum image capable for the display.
The most reasonable video scaler we have found to date is the DVDO Edge from
Anchor Bay. See www.anchorbaytech.com/dvdo_edge/
PRINTING MICROSCOPE PICS FROM YOUR COMPUTER:
Photo printers abound in the marketplace from Canon, Sony, HP, Kodak and Epson. A
few of these are ink based, some may be dye sublimation. We really like the dye
sublimation printers, These models print 4x6 photo quality prints and uses a dye
sublimation film pack that has the ink - cyan, magenta, yellow, black - on a roll of film.
One film pack with paper is good for 50 prints that put out consistent quality on every
print. Cost is about $.25 per print.
Note on printing:
For standard definition imaging, the software that comes with the PCTV Tuner box
that sits between the microscope camera and computer will allow you to easily capture
images - either JPEG, or moving live action AVI type files. One would use the standard
windows file explorer to create client folders where the images could be saved. Images
from those folders can then be printed one at a time using the standard windows file
and fax viewer or by using the software that comes with the printer. With this software
images could be manipulated and printed. For example, one 4x6 print could contain 2,
4, 8, or more separate microscope images.
For HD cameras, you will likely be snapping pics with your camera’s picture taking
ability and then download those images to your computer through the USB connection.
Once in your computer you can manipulate them any way you desire using software
that came with your camera, computer or some third party. You can also just pull the
SD memory card from your camcorder (if using a camcorder with this option) and plug
it into a computer’s card reader and print directly from there.
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GOING WIRELESS
With the HD camcorders, we typically use memory cards to temporarily store the
pictures we have taken. One type of “wireless” memory card is from
Eye-Fi. With this card installed in one of your memory card slots
(slot B if using Canon dual slot memory camcorders) images that
are taken with your camcorder can automatically be transferred
through your network right to your computer so no USB cable hookup is required. This is kind of nifty. Take a photo of an interesting
specimen and it shows up in your computer within the Eye-Fi
software so you can then do what you want with it.
Instructions for installation and use of these devices is within the
manual for the particular Eye-Fi card you might have.
Note: If we shipped your microscope with a camcorder, you might have the Eye-Fi chip
installed in the camera as an option. You will need to take this out of the camera and put
it into the Eye-Fi USB reader and install the contained software on your computer. See
the Eye-Fi chip instruction manual for details.
BYPASSING THE COMPUTER WITH STANDARD DEF. CAMERAS
If you are gadget and computer phobic and would prefer to print directly to a printer from
the video camera, you will need a dye sublimation video photo printer. Printers that print
from the computer are called video digital printers, and those that can bypass the
computer and print directly from the video are called video photo printers. Right now
these take a standard video camera output. To date we have not seen any that take an
HDMI output direct from a high def camcorder.
Expect to pay about $1300-1400 for one of these depending upon specs.
BYPASSING THE COMPUTER WITH HD CAMERAS
When you capture an image to a memory chip with an HD camera, that chip can be
removed and plugged directly into many digital printers. You will be able to read what is
on the chip as well as print photos without the need for a computer. Refer to the
operating guide of your selected printer.
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Standard Definition
Video Camera
SI-C400N / SI-C500N / SI-C500P
- Standard Definition Camera -
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PAL Format Cameras: 1/50
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PAL 15.625kHz
PAL 50Hz
PAL Format Cameras 752(H) X 582
TIPS FOR HD CAMCORDERS
First, there is no getting around it, you should read your camcorder manual.
Since we are using the camcorder on a microscope, there will be a whole lot of things that
are on your camera that you will likely never be using and you will be reading about those
things in the manual—but nevertheless you should become familiar with the unit and read the
manual anyway.
The single most important thing to remember is that camcorders have exposure controls
which can be shutter or aperture priority. You might find that setting your camera to aperture
priority mode works best with the aperture at the lowest up to about 5.6 setting. Programmed
AE mode is a default selection with most of the Canon cameras, we usually use aperture
priority mode. The systems when coming from Biomedx are pre-set for this. If you use shutter
priority you may notice your viewing field will be darker on the top or bottom half of the
screen vs. the other side. This is the electronic shutter attempting to shut down on some of
the light entering the camera.
Here are some pointers on camera settings looking at one Canon system. There are
similarities between all Canon camera interfaces. Page 58 shows another model. Scan both
of these samples and you may find one or the other of these samples is closer to what you
have on your system.
When your camera is on, your screen may look like this.
This tells you that you are in video
mode. This is the mode for normal
scope viewing.
Pressing “FUNC.”
brings you to the
camera function listings.
You will want to make
some setting changes
here.
Pressing “MENU” will
bring you to the camera
menu settings. You’ll
also want to make
changes here.
What shows up in this spot
where “MENU” appears can be
different depending upon the
last function you used in the
FUNC screen.
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Pressing the camera
icon here will shift you
to camera mode of the
camcorder.
Page 58
Here is the FUNC panel display.
Things you might change:
Choose WB - White Balance.
Then choose Custom WB.
Then Set WB.
You MUST go to Rec. Program
and another screen will appear.
The easy way to do it is to go to a phase
contrast live blood view and set the
white balance from that screen. Another
way is to switch to brightfield mode with
no slide, have your light source set at
about 11:00 position and then set WB.
The default is to leave it in auto mode.
You need to set the camera to Programmed AE. If
you are viewing a live blood image in phase
contrast and it appears as though half of the
screen goes a bit dark, this is the auto shutter
engaging. You need to turn down the light. Setting
the mode to Aperture Priority will eliminate this (set
the aperture to the lowest number), but when
viewing a dry layer in bright field mode with a wide
field view, the image will appear washed out. You
could change the aperture setting or just go to
Programmed AE mode
Scrolling down the FUNC panel
displays more options.
There are items you will want to set in the
MENU tab.
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Here are the MENU screens (selected from the FUNC display).
Here are sample settings. Notice that many things are
turned OFF as you will not be using these functions during
microscope sessions.
On the screen shown to the left, note “Image Effects” at
the bottom. From here choosing Custom Effect will allow
you to tweak the image on screen a bit in terms of contrast, brightness, color and sharpness.
Always remember that your HD display monitor will also
have settings that you should get into and tweak for your
most ideal display image.
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It is important to read your camera manual so you know where to plug in the
power cord, where to place the battery, how to insert and remove memory cards,
where to plug in your HDMI cable, etc.
Shown here is one model.
HDMI cable input is on other
side under hand strap.
DC Power in
On the side of your camera (in the Canon series) you will
have a button that appears as shown here. Pressing this will
shift you to the memory mode so you may view, edit and
delete the images contained on the memory chip(s).
Memory cards go
here. On some
models the cards
may be inserted
on the back side
of the camera.
There is usually a switch near the top, back or side of the camera with 3
positions like; Auto mode / Manual mode / Cinema mode. The manual mode is
noted by the letter “M” on some models and would be the position for most
flexibility with the microscope.
See next page for another model.
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Here’s another model as you’d see it sitting on
your microscope. The memory chip slides into the
bottom of the unit under the cover door.
This is an important button on
this unit as pressing it brings
you to a key mode selection
panel screen.
Main Functions bring you to the screen to set white balance and
other functions.
Camera Mode is important as you will want to be in Manual Mode
and set things up best for the scope.
Other Setting is important to turn off things that are sometimes
automatic which you don’t want on.
The Recording Standard lets you set your recording mode, from
very high quality AVCHD for TV and editing to MP4 which is good
for most video recording as this format can easily be uploaded
online for web viewing.
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This is generally what the settings on your
panels will look like in the Other Settings
mode.
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Here are two items in the main functions
you will want to know about.
It is the top two on the screen, the
Rec. Programs and WB white
balance.
When you are looking at a specimen on the
scope, the color temperature may not give the
best rendering to the video screen. If viewing live
blood for instance in phase contrast, and you
have a view on screen with the light turned up
about 60-70 %, you can go to white balance
mode and go to manual set, and when pushing
that option the camera will adjust the color
balance. This is usually all it takes and it will also
be good for locking in a good color for dry layer
blood images as well.
For the Rec. Programs, it is best to have this set
for Av which is Aperture priority mode and itseem
to work best with lower aperture settings.
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Remember that your zoom control is on
top of the camera which is on the
opposite side of you when you are
looking into your microscope. When you
reach up with your right hand to the side
of the camera your index finger will rest
right on it.
You may also obtain a remote control
and turn on the camera remote option in
the other settings menu system and use
the remote to zoom in and out on your
image.
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