National 158-LED Instructions Manual

National Optical & Scientific Instruments Inc.

11113 Landmark 35 Drive

San Antonio, Texas 78233

Phone (210) 590-9010 Fax (210) 590-1104

INSTRUCTIONS FOR

MODEL 158-LED

COMPOUND MICROSCOPES

Copyright © 11/09/04

National Optical & Scientific Instrument Inc.

Widefield 10x/18 eyepiece

Interpupillary scale

Viewing head of microscope

Revolving nosepiece

Objective lenses

Specimen holder (mechanical stage)

Stage

Abbe condenser 1.25 N.A.

Iris diaphragm lever

Swing-out filter holder

Illuminator condenser

Base

On/Off switch

Knurled diopter rings

(left & right sides)

Sliding interpupillary adjustment, grips located on both left and right side of diopter scale

Mark on side of eyepiece tube for indexing diopter reading

Head locking screw

Arm of microscope stand

Rack stop adjustment screw

Knurled locking screws for securing specimen holder to stage

Tension adjustment collar

(behind focus knob on left side)

Coarse focus knob

Knobs controlling X and Y movement of mechanical stage

Fine focus knob

Power on/off switch

Light intensity control knob

Recharging socket

INTRODUCTION

Thank you for your purchase of a National microscope. It is a precision instrument carefully checked to assure that it reaches you in good condition. It is designed for ease of operation and years of carefree use. The information in this manual probably far exceeds what you will need to know in order to operate and maintain your microscope. However, is provided to answer questions that might arise, and to help you avoid any maintenance expense that may be unnecessary.

Your new compound microscope is a high performance microscope with high quality achromatic objective lenses that provide good resolution and optical centering. The microscope is designed with a built-in mechanical stage providing a travel range of

75mm x 30mm in the X and Y direction with graduation reading up to 0.1mm for accurate positioning of specimen. Also included is a ball bearing quadruple nosepiece, separate coarse and fine focusing, spiral mounted N.A. 1.25 Abbe condenser, and built-in heat free LED illumination equal to a 20 watt bulb.

Carefully read these instructions before operating microscope. They will permit you to use your new microscope to its fullest capability. Referring to diagram on page 2 identifies nomenclature used to describe components and controls.

UNPACKING

1. The microscope and accessories have been carefully packed to assure that they reach you in the best possible condition.

Check package containers to make sure all components are accounted for. a. Microscope stand with the following components already installed WF10x eyepieces (pair), 4 objectives, condenser, and three rechargeable batteries. b. Two rubber eyeshields, specimen holder, recharger, dust cover, 0.9mm “L” type key wrench (for lamp replacement), and 2mm “L” type key wrench (for rack stop adjustment), and instruction manual.

2. Save packing container in case it becomes necessary to ship the microscope for any reason, repack in the styrofoam container, and then pack the styrofoam in other corrugated shipping container for optimum protection. Use of the styrofoam alone will not provide adequate protection in transit, and will void your warranty.

DESCRIPTION OF COMPONENTS

1. EYEPIECE (ocular lens): Lens closest to the eye, magnifies the primary image formed by the objective lens. One eyepiece is equipped with a “ pointer” that rotates as the eyepiece is turned.

2. OBJECTIVE TURRET (nosepiece): Revolving turret which holds objective lenses, permits changes of magnification by rotating different powered objective lenses into optical path. Reverse position permits easier access to stage when positioning specimen slides.

3. OBJECTIVE LENS: Lens closest to the object being viewed, forms first magnified image of the specimen.

4. MECHANICAL STAGE: Permits precise, mechanical manipulation of the specimen slide.

5. SPECIMEN HOLDER: Holds specimen slide.

6. STAGE: Platform of the microscope where the specimen slide is placed.

7. CONDENSER: A focusable 1.25 N.A. Abbe condenser lens positioned under center of stage condenses light rays from substage illumination and fills the back lens element of objective lens to improve image resolution.

8. IRIS DIAPHRAGM: Attached to bottom of Abbe condenser, controls aperture of light by moving control lever left or right.

9. FILTER HOLDER: Attached to bottom of iris diaphragm with swing out built in neutral filter. Swing out filter should be removed from optical path when using 40x or 100x objective lens.

10. SAFETY RACK STOP: When properly adjusted, controls maximum upward travel of stage. Prevents higher power objectives from breaking specimen slides, prevents damage to objective lenses. This stop has been pre-adjusted at the factory.

11. FOCUSING KNOBS: Coarse focusing knobs (larger knobs) located on each side of arm, raise or lower stage to bring specimen image into focus. Fine focus knobs (smaller knobs located just below coarse focusing knobs) permit more precise image adjustment.

12. ILLUMINATION: Built-in substage LED illuminator provides constant, reliable pre-focused illumination.

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ASSEMBLY

1. Specimen holder: a. Rotate coarse focusing knob to move stage platform to its lowest position. b. Remove two knurled screws from mechanical stage platform. c. Place specimen holder on stage and using the two knurled locking screws, attach specimen holder to mechanical stage.

2. Rubber eye shields a. Eye shield is designed with two diameters, the largest is for mounting over the eyepiece. b. Slip rubber eye shield over top edge of eyepieces, with smallest diameter facing user. c. These shields help block out undesired light reflections, and to position your eyes at the proper point above the eyepieces.

OPERATION

1. Place microscope directly in front of you in a manner, which permits you to comfortably look into the eyepieces. Note that the head of microscope rotates 360 º , permitting you to operate the microscope from the front or from the back, whichever is most convenient for you. It also permits convenient sharing of microscope by more than one user, by simply rotating head, without needing to move entire microscope.

2. Assure that light is available for illuminating the specimen. a. Your microscope has special LED illumination that is powered by 3 rechargeable AA nickel metal hydride batteries

(supplied). These batteries may be recharged, as required, using the recharger (supplied). Each set of batteries may be recharged approximately 500 times before replacing, and each charge will provide up to 50 hours of microscope operation. The LED component (bulb) will last for up to 50,000 hours before replacement is required.

WARNING

DO NOT USE regular AA alkaline batteries. Use of other than rechargeable AA nickel metal hydride batteries could result in batteries exploding during recharge. ONLY USE THE SUPPLIED SWITCHING BATTERY

RECHARGER WITH AUTOMATIC “TRICKLE CHARGE”. b. It is recommended that you charge the batteries before initial use and after prolonged storage as the batteries may have discharged. Plug output cord from battery charger into DC recharging socket located on back of microscope base. Your automatic switching recharger operates on 100 to 240 volts AC 50/60 Hz. Plug charger into your AC wall outlet. Battery recharger is equipped with an automatic “trickle charge” feature, the red LED indicator lamp located on recharger will be illuminated when batteries are receiving maximum charge. After batteries are charged, the red LED indicator lamp will turn to green and charger automatically switches to “trickle charge”. The charger can be left plugged in, but for safety reasons it is a good idea to disconnect the charger from the AC wall outlet and the output cord from recharging socket after 12 hours. Batteries and charger may feel warm when charging, and unplugging the recharger is a safety precaution.

Note that your microscope can be used during recharging. Simply turn “on/off” switch on back of microscope base to

“on” position and proceed as follows. In case of equipment malfunction, see “Troubleshooting” procedure.

2. Interpupillary adjustment of binocular viewing head. a. Look through microscope and adjust distance between the two eyepiece tubes by grasping the sliding mounts to left and right of eyepieces and sliding together or apart. b. When a full field of view is observed through both tubes, and images blend into one, interpupillary distance is corrected for your eyes. Check the interpupillary scale and note index reading for future reference, in case other users will be changing this adjustment from time to time. c. Adjust the diopter scales, located on each eyepiece tube, to the same numerical value as indicated on the interpupillary scale. This must be done in order to maintain parfocality of objective lenses. If interpupillary distance is changed, adjust eyepiece diopters accordingly.

3. Focusing the microscope. a. Position the 4x objective lens into the optical path, making sure that lens is properly indexed in its click-stop position. b. Swing moveable finger on specimen slide holder outward. Place specimen slide (cover slip up) on top of stage surface against fixed side of slide holder. Slowly release moveable finger until it makes contact with specimen slide.

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c. Rotate coarse focusing controls until specimen comes into focus. d. Adjust fine focus controls until specimen is in sharp focus. e. Adjust diopter for possible difference in eyesight between user’s left and right eyes. If user has 20/20 vision, this adjustment should not be necessary.

1. Using right eye, peer into the right eyepiece tube. Adjust sharpness of image by utilizing fine focus controls, without touching the diopter ring.

2. Using left eye, peer into the left eyepiece tube. Adjust sharpness of image by turning diopter adjustment located on left eyepiece tube. f. Adjusting the aperture (opening) of iris diaphragm.

Iris diaphragm should not be used to control the brightness of illumination, use the illuminator light intensity control to adjust light level. Iris diaphragms are designed to help achieve high resolution of specimen and provide contrast in the image. Smaller apertures will deliver higher contrast to image. However, closing aperture too much will reduce resolution. Experimentation is the best method of determining the correct opening of diaphragm. Some suggested openings for iris diaphragm are:

OBJECTIVE

4x

10x

40x

DIAPHRAGM OPENING

From fully closed to 1/8 open

1/8 to 1/4 open

1/4 to 1/2 open

1/2 to 3/4 open 100x g. Usage of special built-in neutral filter.

1. Placing a filter into the optical path will absorb some of the light from the illuminator base, limiting the light output.

2. When using 4x and 10x objectives the special neutral filter must be in optical path.

3. When using the higher power 40x and 100x objectives remove filter from optical path.

4. To remove filter, grasp filter holder knob and swing out filter holder. h. Changing magnification.

1. Rotate revolving nosepiece to position 10x objective into optical path.

2. This microscope has been parfocalled, which allows changes from one objective to another while requiring only a slight adjustment of the fine focus controls.

3. When changing to the 40x and 100x objective lens, care must be exercised when positioning these lenses into the optical path, in order to prevent damaging the front lens element and specimen slide.

4. In order to obtain maximum resolution of the 100x oil immersion lens, it is necessary to apply immersion oil between the cover glass of slide and front lens of the objective. a) Use of a very small amount of immersion oil is required. b) All air bubbles must be removed from between lens and slide by gently rotating nosepiece back and forth. c) When finished viewing, all parts that come in contact with oil must be cleaned. Failure to do so could permanently damage the 100x oil immersion objective lens. Use Windex to clean immersion oil off lens surfaces is recommended.

Objective

Din 4X

Din 10X

Din 40X

Din 100X

N.A.

0.10

0.25

0.65

1.25

Objective Specification Chart

Color Code

Ring

Field of

View

Red

Yellow

Blue

White

4.5mm

1.8mm

0.45mm

0.18mm

Working

Distance

26.4mm

5.5mm

0.48mm

0.06mm

Magnification with

WF10X eyepiece

40X

100X

400X

1000X

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MAINTENANCE

WARNING: For your own safety, turn power switch off, remove plug from AC power source and remove charging cord from microscope input before maintaining your microscope. If the power cord, recharger is worn, cut or damaged in any way, have it replaced immediately to avoid shock or fire hazard.

1. OPTICAL MAINTENANCE a. Do not attempt to disassemble any lens components. Consult a microscope service technician when any repairs not covered by instructions are needed. b. Prior to cleaning any lens surface, brush dust or lint off lens surface using a camel hairbrush. You can also use an ear syringe or canned compressed air, such as that sold by most computer stores. c. Do not remove eyepieces or objective lenses to clean. Clean only the outer lens surface. Breath on lens to dampen surface, then wipe with lens paper or tissue or use a cotton swab moistened with distilled water. Wipe lenses with a circular motion, applying as little pressure as possible. Avoid wiping dry lens surface as lenses are scratched easily.

If excessive dirt or grease gets on lens surfaces, a small amount of Windex can be used on a cotton swab or lens tissue. To clean objective lenses, do not remove objectives from microscope. Clean front lens element only, following same procedure.

NOTE: Fingerprints or other matter on the front lens element of the objective lens is the single most common reason that you will have difficulty in focusing the microscope. Before having costly servicing done, or before returning to

National for “warranty repair”, make certain to examine the front lens element with a magnifying glass or eye loupe for the presence of such contaminants. If a microscope is returned to National for warranty repair, and it is determined that such contaminants are the problem, this is not covered under warranty and National will submit a cost estimate for cleaning.

2. MECHANICAL MAINTENANCE a. The rack stop screw has been pre-adjusted at the factory and should not require re-adjustment. However, if you do attempt re-adjustment, note the following procedure.

Loosen round knurled locking nut by turning counter clockwise then, using a 2mm “L” type hex key wrench, loosen rack stop socket set screw. With fine focus adjustment at mid-range, focus on standard slide until sharp image is obtained. Rotate rack stop set screw in clockwise direction until tight, tighten locking nut.. b. Coarse focus tension adjustment prevents the stage from drifting down from its own weight and causing the image to move out of focus. This has been adjusted at the factory, but over the course of time it may loosen and cause the stage of the microscope to slip downward on the focusing block.

The tension adjustment collar is located between arm and coarse focus knob on right side of microscope. Using a

0.9mm “L” type hex key wrench, loosen the set screw located in the hole on tension adjustment collar. Turn collar clockwise to tighten tension, counter-clockwise to loosen tension. Use of a wide rubber band will provide a better grip on the tension adjustment collar. After adjusting, tighten the setscrew to lock collar in place.

NOTE: It is recommended that you leave the tension as loose as possible for ease of focusing, yet not so loose that it permits the stage of microscope to drift downward from its own weight and cause the microscope to “drift” out of focus. c. Metal parts: Use a clean, damp cloth to remove dust or dirt from metal parts, followed by a dry cloth.

3. ELECTRICAL MAINTENANCE a. The extent of electrical maintenance, by other than a qualified technician, should be LED replacement, battery recharging and battery replacement. Before maintenance, be sure that recharger is not connected to microscope. b. Recharging batteries:

Plug output cord from battery charger into DC recharging socket located on back of microscope base. Your automatic switching recharger operates on 100 to 240 volts AC 50/60 Hz. Plug charger into your AC wall outlet. Battery recharger is also equipped with an automatic “trickle charge” feature, the red LED indicator lamp located on recharger will be illuminated when batteries are receiving maximum charge. After batteries are charged, the red LED indicator lamp will turn to green and charger automatically switches to “trickle charge”. The charger can be left plugged in, but for safety reasons it is a good idea to disconnect the charger from the AC wall outlet and the output cord from recharging socket after 12 hours. Batteries and charger may feel warm when charging, and unplugging the recharger is a safety precaution.

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c. Replacing batteries:

Your microscope includes 3 rechargeable AA nickel metal hydride batteries. These may be recharged up to 500 times, but if you observe that a recharge is providing significantly less than 40 hours of operation. It is probably time to replace to batteries.

Note that your microscope can be used during recharging. Simply turn “on/off” switch on back of microscope base to

“on” position and proceed as follows. In case of equipment malfunction, see “Troubleshooting” procedure.

IMPORTANT WARNING: DO NOT USE REGULAR ALKALINE BATTERIES IN THIS MICROSCOPE. ANY

ATTEMPT TO RECHARGE ALKALINE TYPE BATTERIES COULD RESULT IN BATTERIES EXPLODING.

Gently lay microscope on its side. Using a Phillips (cross head) type screwdriver, remove 5 each 3mm Phillips screws from bottom of base. Remove base, observe black plastic battery case located on bottom of base. Carefully remove

Phillips screw that holds battery case together. Slide lid of case straight out to remove and expose batteries. Remove all 3 batteries and replace with new rechargeable AA nickel metal hydride batteries, making certain to insert with correct polarity according to markings on battery holder. Replace battery case lid and install base to microscope.

Follow instructions on new battery packaging to determine if they are already charged, or if they should be charged before initial use. If recharging is required, following directions in “3.b” above. d. Replacing LED element:

An LED “bulb” will last up to 50,000 hours, so you don’t have to do this exercise very often.

To open the illuminator field lens housing, use hex wrench supplied with your microscope to loosen hex screw on side of lens housing. Remove lens housing to expose LED “bulb.” Remove bulb by grasping the plastic base of bulb and gently pulling straight up. Insert new LED “bulb”, replace lens housing and tighten hex screw to secure lens housing in place.

TROUBLESHOOTING

PROBLEM

Light fails to operate.

Image does not remain in focus

Image does not focus

Poor resolution

(image not sharp)

REASON FOR PROBLEM SOLUTION

Batteries fully discharged.

Batteries fully discharged and will not recharge.

Rheostat control not turned far enough.

Light switch in off position.

LED “bulb” burned out.

Recharge batteries.

Replace batteries or replace charger.

Turn rheostat to increase light intensity.

Turn light switch on.

Replace LED “bulb”.

Adjust tension control Stage of microscope drops from its own weight.

Slide upside down.

Slide cover slip too thick.

Objective lenses dirty.

Eyepiece lens dirty.

Too much light, iris diaphragm not adjusted properly.

Too much light, iris diaphragm

Not adjusted properly.

Too much light when using 4x and

10x objective lenses, swing out filter not in optical path.

Place slide on stage with cover slip up.

Use 0.17mm thick cover slip

(No.1 cover slip)

Clean objective lenses.

Clean eyepiece lenses.

Adjust iris diaphragm.

Adjust iris diaphragm.

Place swing in filter into optical path.

Spots in field of view. Eyepiece or condenser lens dirty.

Specimen slide dirty.

Clean lens. ***

Clean slide.

***Spots in field of view can also result from dirt on inside of eyepiece. It is recommended that you have service technician clean inside of lens.

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OPTIONAL ACCESSORIES AND PARTS:

#610-045

#610-045R

#800-001

WF15x eyepiece w/pointer

WF10x eyepiece with reticle, 10mm/100 divisions

Replacement LED light

#802-003

#951

#975-001

Auto cut-off recharger for rechargeable LED microscope.

Dustcover, 16” tall x 13”, heavy-duty vinyl with stitched seams

Carrying case anodized aluminum, fabric lining, accessory pockets, Velcro straps, keyed lock.

LIMITED LIFETIME WARRANTY

National warrants this microscope to be free from defects in material and workmanship under normal use and service for the life of the instrument. This limited lifetime warranty excludes all internal electrical components which are only warranted for a period of one year. The warranty does not cover damage resulting from abuse or misuse, repairs or alterations performed by other than authorized repair technicians, or damage occurring in transit. The warranty does not cover bulbs, power supplies, rechargers, batteries, fuses, cords, or add-on accessories such as mechanical specimen holders that are not built into the microscope stage as an integral part of original manufacture.

Warranty does not cover lenses that have become inoperable due to excessive dirtiness as a result of misuse or lack of normal maintenance.

Any cameras and software supplied with this microscope are warranted from date of purchase as follows:

Camera, 1-year limited warranty: Manufacturer warrants camera to be free from defects in material and workmanship under normal use and service for 1 year from date of purchase. It does not cover damage resulting from abuse or misuse, repairs or alterations performed by other than the manufacturer, or damage occurring in transit.

Software, 90-day limited warranty: Manufacturer warrants software to be free from defects in material and workmanship under normal use and service for 90 days from date of purchase.

Other than set forth above, National hereby disclaims all warranties, express or implied, of fitness for a particular purpose.

While it is not necessary to register your purchase of a microscope, any camera or software must be registered by completing and mailing the warranty registration card enclosed with such product.

For warranty service, instrument should be well packed to avoid damage in transit, accompanied by a description of the difficulty and return instructions, and shipped postage prepaid to National at the address below. National will repair or replace at no charge and return postage prepaid . For customers living outside the United States, National will provide standard warranty service. However, inbound & outbound shipping cost is the responsibility of the consumer.

If failure was caused by misuse, alterations, accident or abnormal conditions of operation, an estimate for repairs will be submitted for approval prior to work being performed.

This warranty gives you specific legal rights, and you may also have other rights that vary from state to state. If you have questions concerning this product or warranty, contact the dealer from which it was purchased. You may also contact National at the address and phone number printed on the front of this manual.

(Revised 9/14/10)

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