Agilent 1260 Infinity Bio-inert High Performance Autosampler

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Agilent 1260 Infinity
Bio-inert High Performance
Autosampler
User Manual
Agilent Technologies
Notices
© Agilent Technologies, Inc. 2011
Warranty
No part of this manual may be reproduced
in any form or by any means (including electronic storage and retrieval or translation
into a foreign language) without prior agreement and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
The material contained in this document is provided “as is,” and is subject to being changed, without notice,
in future editions. Further, to the maximum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a particular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or performance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the separate agreement shall control.
Manual Part Number
G5667-90000
Edition
03/2011
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a component of an in vitro diagnostic system if the system is registered with
the appropriate authorities and complies with the relevant regulations.
Otherwise, it is intended only for general laboratory use.
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly performed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated conditions are fully understood and
met.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accordance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of a
U.S. Government prime contract or subcontract, Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in FAR
2.101(a) or as “Restricted computer software” as defined in FAR 52.227-19 (June
1987) or any equivalent agency regulation
or contract clause. Use, duplication or disclosure of Software is subject to Agilent
Technologies’ standard commercial license
terms, and non-DOD Departments and
Agencies of the U.S. Government will
WA R N I N G
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indicated conditions are fully understood and met.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
In This Guide
In This Guide
This manual covers the Agilent 1260 Infinity Bio-inert High Performance
Autosampler (G5667A)
1 Introduction
This chapter gives an introduction to the autosampler, an overview of the
instrument, and a description of internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Autosampler
This chapter provides information on unpacking, checking on completeness,
stack considerations and installation of the autosampler.
4 LAN Configuration
This chapter provides information on connecting the autosampler to the
Agilent ChemStation PC.
5 Using the Module
This chapter provides information on how to set up the autosampler for an
analysis and explains the basic settings.
6 Optimizing Performance
This chapter gives hints on how to optimize the performance or use additional
devices.
7 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
3
In This Guide
8 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
9 Test Functions and Calibration
This chapter describes the tests for the module.
10 Maintenance
This chapter describes the maintenance of the Autosampler
11 Parts for Maintenance
This chapter provides information on parts material required for the module.
12 Identifying Cables
This chapter provides information on cables used with the 1260 series of
HPLC modules.
13 Hardware Information
This chapter describes the autosampler in more detail on hardware and
electronics.
14 Appendix
This chapter provides addition information on safety, legal and web.
4
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Contents
Contents
1 Introduction
9
Features 10
Bio-inert Materials 11
Overview of the Module 13
Autosampler Principle 15
Early Maintenance Feedback 21
Instrument Layout 22
2 Site Requirements and Specifications
23
Site Requirements 24
Physical Specifications 27
Specifications 28
3 Installing the Autosampler
31
Unpacking the Autosampler 32
Optimizing the Stack Configuration 34
Installing the Autosampler 39
Flow Connections to the Autosampler 41
Installation of stainless steel cladded PEEK capillaries
4 LAN Configuration
43
Setting up the module in a LAN environment
Connecting the module via LAN 45
5 Using the Module
42
44
47
Preparing the Autosampler 48
Setting up the Autosampler with Agilent ChemStation 52
Main Screens of the Autosampler with Agilent Instant Pilot (G4208A)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
62
5
Contents
6 Optimizing Performance
65
Delay Volume and Extra-Column Volume 66
How to Configure the Optimum Delay Volume 67
How to Achieve Higher Injection Volumes 69
How to Achieve High Throughput 71
How to Achieve Higher Resolution 72
How to Achieve Higher Sensitivity 75
How to Achieve Lowest Carry Over 76
7 Troubleshooting and Diagnostics
79
Overview of the Module’s Indicators and Test Functions
Status Indicators 81
User Interfaces 83
Agilent Diagnostic Software 84
8 Error Information
80
85
What are Error Messages 87
General Error Messages 88
Module Error Messages 94
9 Test Functions and Calibration
Introduction 106
System Pressure Test 107
Sample Transport Self Alignment
Maintenance Positions 111
Injector Steps 115
6
105
109
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Contents
10 Maintenance
119
Introduction to Maintenance 120
Warnings and Cautions 121
Overview of Maintenance 123
Cleaning the module 124
Removing the needle assembly 125
Installing the needle assembly 128
Exchanging the Needle Seat 131
Replacing the Rotor seal 134
Removing the metering seal 138
Installing the metering seal 141
Replacing Peristaltic Pump Cartridge 143
Installing the Interface Board 146
Replacing the Module Firmware 147
11 Parts for Maintenance
149
Overview of Maintenance Parts 150
Vial Trays 151
Recommended Plates and Closing Mats
Recommended Vial Plates 153
Accessory Kit 154
12 Identifying Cables
152
155
Cable Overview 156
Analog Cables 158
Remote Cables 160
BCD Cables 163
CAN/LAN Cables 165
External Contact Cable 166
Agilent Module to PC 167
Agilent 1200 Module to Printer
168
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
7
Contents
13 Hardware Information
169
Firmware Description 170
Interfaces 173
Setting the 8-bit Configuration Switch
Electrical Connections 183
14 Appendix
179
185
General Safety Information 186
Lithium Batteries Information 189
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 190
Radio Interference 191
Sound Emission 192
Use of Solvents 193
Agilent Technologies on Internet 194
8
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
1
Introduction
Features
10
Bio-inert Materials
11
Overview of the Module
Autosampler Principle
13
15
Early Maintenance Feedback
Instrument Layout
21
22
This chapter gives an introduction to the autosampler, an overview of the
instrument, and a description of internal connectors.
Agilent Technologies
9
1
Introduction
Features
Features
The Agilent 1260 Infinity Bio-inert High Performance Autosampler features an
increased pressure range (up to 600 bar) enabling the use of today’s column
technology (sub-two-micron narrow bore columns) with the Agilent 1260
Infinity LC System. Increased robustness is achieved by optimized new parts,
high speed with lowest carry-over by flow through design, increased sample
injection speed for high sample throughput, increased productivity by using
overlapped injection mode and flexible and convenient sample handling with
different types of sample containers, such as vials and well plates. Using
384-well plates allows you to process up to 768 samples unattended.
For specifications, see “Specifications” on page 28
NOTE
10
This 1260 Infinity Autosampler has been introduced together with the Agilent 1260 Infinity
Liquid Chromatograph.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Introduction
Bio-inert Materials
1
Bio-inert Materials
For the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies uses
highest quality materials in the flow path (also referred to as wetted parts),
which are widely accepted by life scientists, as they are known for optimum
inertness towards biological samples and ensure best compatibility to common
samples and solvents over a wide pH range. Explicitly, the complete flow path
is free of stainless steel and free of other alloys containing metals like iron,
nickel, cobalt, chromium, molybdenum or copper, which can interfere with
biological samples. The flow downstream of the sample introduction does not
contain metals at all.
Table 1
Bio-inert Materials
Module
Materials
Agilent 1260 Infinity Bio-inert Quaternary Pump
(G5611A)
Titanium, gold, platinum-iridium,
zirconium oxide, ruby, PTFE, PEEK
Agilent 1260 Infinity Bio-inert High-Performance Autosampler Upstream of sample-introduction:
(G5667A)
• Titanium, gold, PTFE, PEEK,
zirconium oxide
Downstream of
sample-introduction:
• PEEK, zirconium oxide
Agilent 1260 Infinity Bio-inert Manual Injector
(G5628A)
PEEK, zirconium oxide
Agilent 1260 Infinity Bio-inert Analytical Fraction Collector
(G5664A)
PEEK, zirconium oxide, PTFE
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
11
1
Introduction
Bio-inert Materials
Table 1
Bio-inert Materials
Module
Materials
Bio-inert Flow Cells:
Standard flow cell bio-inert, 10 mm, 13 µl, 120 bar (12 MPa)
for MWD/DAD, includes Capillary Kit Flow Cells BIO (p/n
G5615-68755) (p/n G5615-60022) (for Agilent 1260 Infinity
Diode Array Detectors DAD G1315C/D)
PEEK, zirconium oxide, sapphire,
PTFE
Max-Light Cartridge Cell Bio-inert (10 mm, V(σ) 1.0 µl) (p/n
G5615-60018) and
Max-Light Cartridge Cell Bio-inert (60 mm, V(σ) 4.0 µl) (p/n
G5615-60017) (for Agilent 1290 Infinity Diode Array Detectors
DAD G4212A/B)
PEEK, fused silica
Bio-inert flow cell, 8 µL, 20 bar (pH 1–12) includes Capillary Kit
Flow Cells BIO (p/n G5615-68755)) (p/n G5615-60005) (for
Agilent 1260 Infinity Fluorescence Detector FLD G1321B)
PEEK, fused silica, PTFE
Bio-inert heat-exchanger G5616-60050 for 1290 Infinity
Thermostatted Column Compartment
(G1316C)
PEEK (steel-cladded)
Bio-inert Valve heads
G4235A, G5631A, G5639A: PEEK,
ceramic (Al2O3 based)
Bio-inert Connection capillaries
Upstream of sample-introduction:
• Titanium
Downstream of
sample-introduction:
• Agilent uses
stainless-steel-cladded PEEK
capillaries, which keep the flow
path free of steel and provide
pressure stability to more than
600 bar.
For ensuring optimum bio-compatibility of your Agilent 1260 Infinity Bio-inert
LC system, do not include non-inert standard modules or parts to the flow
path. Do not use any parts that are not labeled as Agilent “Bio-inert”. For
solvent compatibility of these materials, see “Solvent information for parts of
the 1260 Infinity Bio-inert LC system” on page 49.
12
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
1
Introduction
Overview of the Module
Overview of the Module
The Autosampler transport mechanism uses an X-Z-theta robot to optimize the
positioning of the sampling arm on the well plate. Once the sampling arm is
positioned over the programmed sample position, the programmed sample
volume is drawn by the metering device into the sampling needle. The
sampling arm then moves to the injection position where the sample is flushed
onto the column.
The Autosampler employs a vial/plate pusher mechanism to hold down the
vial or the plate while the needle is drawn back from the sample vessel (a must
in case a septum is used). This vial/plate pusher employs a sensor to detect
the presence of a plate and to ensure accurate movement regardless of the
plate used.
All axes of the transport mechanism (x-,z-,theta-robot) are driven by
stepper-motors. Optical encoders ensure the correct operation of the
movement.
The standard metering device provides injection volumes from 0.1 – 100 µL.
The entire flow path including the metering device is always flushed by the
mobile phase after injection for minimum internal carry-over.
An additional needle flush station with a peristaltic pump is installed to wash
the outside of the needle. This reduces the already low carry-over for very
sensitive analysis.
The bottle containing the mobile phase for the wash procedure will be located
in the solvent bottle cabinet. Produced waste during this operation is
channeled safely away through a waste drain.
The six-port (only 5 ports are used) injection valve unit is driven by a
high-speed hybrid stepper motor. During the sampling sequence, the valve unit
bypasses the autosampler, and connects flow from the pump to the column
directly. During injection and analysis, the valve unit directs the flow through
the autosampler which ensures that the entire sample is injected onto the
column, and that the metering unit and needle are always free from sample
residue before the next sampling sequence begins.
Control of the vial/plate temperature in the thermostatted autosampler is
achieved using an additional Agilent 1290 Infinity Series module; the Agilent
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
13
1
Introduction
Overview of the Module
1290 Infinity Series thermostat for ALS/FC/Spotter. The thermostat contains
Peltier-controlled heat-exchangers. A fan draws air from the area above the
sample vial tray of the autosampler. It is then blown through the fins of the
cooling/heating module. There it is cooled or heated according the
temperature setting. The thermostatted air enters the autosampler through a
recess underneath the special designed sample tray. The air is then
distributed evenly through the sample tray ensuring effective temperature
control, regardless of how many vials are in the tray. In cooling mode
condensation is generated on the cooled side of the Peltier elements. This
condensed water is safely guided into a waste bottle for condensed water.
14
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Introduction
Autosampler Principle
1
Autosampler Principle
The movements of the autosampler components during the sampling sequence
are monitored continuously by the autosampler processor. The processor
defines specific time windows and mechanical ranges for each movement. If a
specific step of the sampling sequence is not completed successfully, an error
message is generated. Solvent is bypassed from the autosampler by the
injection valve during the sampling sequence. The needle moves to the desired
sample vial position and is lowered into the sample liquid in the vial to allow
the metering device to draw up the desired volume by moving its plunger back
a certain distance. The needle is then raised again and moved onto the seat to
close the sample loop. Sample is applied to the column when the injection
valve returns to the mainpass position at the end of the sampling sequence.
The standard sampling sequence occurs in the following order:
1 The injection valve switches to the bypass position.
2 The plunger of the metering device moves to the initialization position.
3 The needle lock moves up.
4 The needle moves to the desired sample vial position.
5 The needle lowers into the vial.
6 The metering device draws the preset sample volume.
7 The needle lifts out of the vial.
8 The needle is then moved onto the seat to close the sample loop.
9 The needle lock moves down.
10 The injection cycle is completed when the injection valve switches to the
mainpass position.
If needle wash is required it will be done between step 7 and 8.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
15
1
Introduction
Autosampler Principle
Injection Sequence
Before the start of the injection sequence, and during an analysis, the injection
valve is in the mainpass position. In this position, the mobile phase flows
through the autosampler metering device, sample loop, and needle, ensuring
all parts in contact with sample are flushed during the run, thus minimizing
carry-over.
Figure 1
Mainpass Position
When the sample sequence begins, the valve unit switches to the bypass
position. Solvent from the pump enters the valve unit at port 1, and flows
directly to the column through port 6.
16
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Introduction
Autosampler Principle
Figure 2
1
Bypass Position
The standard injection starts with draw sample from vial. In order to do this
the needle moves to the desired sample vial position and is lowered into the
sample liquid in the vial to allow the metering device to draw up the desired
volume by moving its plunger back a certain distance. The needle is then
raised again and moved onto the seat to close the sample loop. In case of an
injector program several steps are interspersed at this point.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
17
1
Introduction
Autosampler Principle
Figure 3
Flush the Needle
18
Drawing the Sample
Before injection and to reduce the carry-over for very sensitive analysis, the
outside of the needle can be washed in a flush port located behind the injector
port on the sampling unit. As soon as the needle is on the flush port a
peristaltic pump delivers some solvent during a defined time to clean the
outside of the needle. At the end of this process the needle returns to the
injection port.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Introduction
Autosampler Principle
Figure 4
Inject-and-Run
1
Flush the needle
The final step is the inject-and-run step. The six-port valve is switched to the
main-pass position, and directs the flow back through the sample loop, which
now contains a certain amount of sample. The solvent flow transports the
sample onto the column, and separation begins. This is the beginning of a run
within an analysis. In this stage, all major performance-influencing hardware
is flushed internally by the solvent flow. For standard applications no
additional flushing procedure is required.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
19
1
Introduction
Autosampler Principle
Figure 5
20
Inject and Run
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Introduction
Early Maintenance Feedback
1
Early Maintenance Feedback
Maintenance requires the exchange of components which are subject to wear
or stress. Ideally, the frequency at which components are exchanged should be
based on the intensity of usage of the module and the analytical conditions,
and not on a predefined time interval. The early maintenance feedback (EMF)
feature monitors the usage of specific components in the instrument, and
provides feedback when the user-selectable limits have been exceeded. The
visual feedback in the user interface provides an indication that maintenance
procedures should be scheduled.
EMF Counters
EMF counters increment with use and can be assigned a maximum limit which
provides visual feedback in the user interface when the limit is exceeded.
Some counters can be reset to zero after the required maintenance procedure.
Using the EMF Counters
The user-settable EMF limits for the EMF Counters enable the early maintenance
feedback to be adapted to specific user requirements. The useful maintenance
cycle is dependent on the requirements for use. Therefore, the definition of the
maximum limits need to be determined based on the specific operating
conditions of the instrument.
Setting the EMF Limits
The setting of the EMF limits must be optimized over one or two maintenance
cycles. Initially the default EMF limits should be set. When instrument
performance indicates maintenance is necessary, take note of the values
displayed by the EMF counters. Enter these values (or values slightly less than
the displayed values) as EMF limits, and then reset the EMF counters to zero.
The next time the EMF counters exceed the new EMF limits, the EMF flag will be
displayed, providing a reminder that maintenance needs to be scheduled.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
21
1
Introduction
Instrument Layout
Instrument Layout
The industrial design of the module incorporates several innovative features.
It uses Agilent’s E-PAC concept for the packaging of electronics and
mechanical assemblies. This concept is based upon the use of expanded
polypropylene (EPP) layers of foam plastic spacers in which the mechanical
and electronic boards components of the module are placed. This pack is then
housed in a metal inner cabinet which is enclosed by a plastic external
cabinet. The advantages of this packaging technology are:
• virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
• the plastic layers have air channels molded into them so that cooling air can
be guided exactly to the required locations,
• the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
• the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.
22
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
2
Site Requirements and Specifications
Site Requirements
24
Physical Specifications
Specifications
27
28
This chapter provides information on environmental requirements, physical and
performance specifications.
Agilent Technologies
23
2
Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
module.
Power Consideration
The module power supply has wide ranging capabilities and accepts any line
voltage in the range mentioned in Table 2 on page 27. Consequently, there is
no voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WA R N I N G
Module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened and the module is connected to power.
➔ Make sure that it is always possible to access the power plug.
➔ Remove the power cable from the instrument before opening the cover.
➔ Do not connect the power cable to the Instrument while the covers are removed.
WA R N I N G
Incorrect line voltage at the module
Shock hazard or damage of your instrument can result if the devices are connected
to line voltage higher than specified.
➔ Connect your module to the specified line voltage.
24
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
2
Site Requirements and Specifications
Site Requirements
CAUTION
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the power
line at any time.
➔ Make sure the power connector of the instrument can be easily reached and
unplugged.
➔ Provide sufficient space behind the power socket of the instrument to unplug the
cable.
Power Cords
Different power cords are offered as options with the module. The female end
of all power cords is identical. It plugs into the power-input socket at the rear.
The male end of each power cord is different and designed to match the wall
socket of a particular country or region.
WA R N I N G
Absence of ground connection or use of unspecified power cord
The absence of ground connection or the use of unspecified power cord can lead to
electric shock or short circuit.
➔ Never operate your instrumentation from a power outlet that has no ground
connection.
➔ Never use a power cord other than the Agilent Technologies power cord designed
for your region.
WA R N I N G
Use of unsupplied cables
Using cables not supplied by Agilent Technologies can lead to damage of the
electronic components or personal injury.
➔ Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
25
2
Site Requirements and Specifications
Site Requirements
WA R N I N G
Unintended use of supplied power cords
Using power cords for unintended purposes can lead to personal injury or damage of
electronic equipment.
➔ Never use the power cords that Agilent Technologies supplies with this instrument
for any other equipment.
Bench Space
The module dimensions and weight (see Table 2 on page 27) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm
(3.1 inches) in the rear for air circulation and electric connections.
If the bench should carry an Agilent system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
CAUTION
Condensation within the module
Condensation will damage the system electronics.
➔ Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔ If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.
26
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
2
Site Requirements and Specifications
Physical Specifications
Physical Specifications
Table 2
Physical Specifications
Type
Specification
Weight
15.5 kg (35 lbs)
Dimensions (height ×
width × depth)
200 x 345 x 440 mm (8 x 13.5 x 17 inches)
Line voltage
100 – 240 VAC, ± 10%
Line frequency
50 or 60 Hz, ± 5%
Power consumption
300 VA / 200 W / 683 BTU
Ambient operating
temperature
4–55 °C (41–131 °F)
Ambient non-operating
temperature
-40–70 °C (-4–158 °F)
Humidity
< 95%, at 25–40 °C (77–104 °F)
Operating Altitude
Up to 2000 m (6562 ft)
Non-operating altitude
Up to 4600 m (15091 ft)
Safety standards: IEC, CSA,
UL
Installation Category II, Pollution Degree 2 For indoor use only.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Comments
Wide-ranging capability
Maximum
Non-condensing
For storing the module
27
2
Site Requirements and Specifications
Specifications
Specifications
Table 3
Specifications - Agilent 1260 Infinity Bio-inert High-Performance Autosampler
(G5667A)
Type
Specification
Injection range
0.1 – 100 µL in 0.1 µL increments. Up to 40 µL with reduced injection
volume kit (hardware modification required). Up to 1500 µL with
multiple draw (hardware modification required)
Precision
Typically < 0.25 % RSD from 5 – 100 µL. Typically < 0.5 % RSD from 2 –
5 µL. Typically < 0.7 % RSD from 1 – 2 µL volume. Measured with
injections of caffeine in water
Injection Accuracy
1 % (10 µL, n=10)
Pressure range
Up to 600 bar (8700 psi)
Sample viscosity range
0.2 – 5 cp
Sample capacity
2 x well plates (MTP) + 10 x 2.0 mL vials 108 x 2 mL vials in 2 x 54 vial
plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate
plus 10 additional 2 mL vials 54 Eppendorf tubes (0.5/1.5/2 mL) in 2 x
27 Eppendorf tube plates
Injection cycle time
Typically < 17 s using the following standard conditions: Default draw
speed: 100 µL/min. Default eject speed: 100 µL/min. Injection volume:
5 µL
Carry-over
Typically <0.004 %
For measurement conditions see 1, 2, 3
pH-range
1-13 (short term 144)
Sample cooling
Optional with G1330B 4 – 40 °C
Materials in flow path
Upstream of sample-introduction:
• titanium, gold, PTFE, PEEK, zirconium oxide
Downstream of sample-introduction:
• PEEK, zirconium oxide
GLP features
28
Early maintenance feedback (EMF), electronic records of maintenance
and errors
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
2
Site Requirements and Specifications
Specifications
Table 3
Specifications - Agilent 1260 Infinity Bio-inert High-Performance Autosampler
(G5667A)
Type
Specification
Communications
Controller-area network (CAN). RS232C, APG-remote standard,
optional four external contact closures and BCD vial number output
Safety features
Leak detection and safe leak handling, low voltages in maintenance
areas, error detection and display
1
Chromatographic conditions: Column: Agilent ZORBAX SB-C18, 2.1 x 50 mm1.8 µm (p/n
827700-902); mobile phase: A: 0.1 % TFA in water, B: 0.1 % TFA in acetonitrile; isocratic : %B=35 %;
flow rate: 0.5 mL/min; temperature: 30 °C
2
UV-detection: Sample : 1200 ng/µl chlorhexidine (dissolved in mobile phase A), 1 µL injected and
measured on G4212A DAD (10 mm cell); Wavelength: 257 nm +/- 4; ref. 360 nm +/- 16; slit 4 nm, 10
Hz
3
MS-detection: Sample : 50 ng/µl chlorhexidine (dissolved in mobile phase A), 1 µL injected and
measured on Agilent 6460 QQQ (in specified conditions); MRM 1: 505.5 ? 170 (CE: 36 V); MRM 2:
505.5 ? 201.2 (CE: 20 V); fragmentor: 150 V, delta EMV(+): 200 V
4
For solvent compatibility, refer to section "Solvent information for parts of the 1260 Infinity Bio-inert LC system" in the manual
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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2
30
Site Requirements and Specifications
Specifications
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
3
Installing the Autosampler
Unpacking the Autosampler 32
Damaged Packaging 32
Delivery Checklist 32
Autosampler Accessory Kit Contents
Optimizing the Stack Configuration
One Stack Configuration 35
Two Stack Configuration 37
Installing the Autosampler
33
34
39
Flow Connections to the Autosampler
41
Installation of stainless steel cladded PEEK capillaries
42
This chapter provides information on unpacking, checking on completeness,
stack considerations and installation of the autosampler.
Agilent Technologies
31
3
Installing the Autosampler
Unpacking the Autosampler
Unpacking the Autosampler
Damaged Packaging
If the delivery packaging shows signs of external damage, please call your
Agilent Technologies sales and service office immediately. Inform your service
representative that the instrument may have been damaged during shipment.
CAUTION
"Defective on arrival" problems
If there are signs of damage, please do not attempt to install the module. Inspection by
Agilent is required to evaluate if the instrument is in good condition or damaged.
➔ Notify your Agilent sales and service office about the damage.
➔ An Agilent service representative will inspect the instrument at your site and
initiate appropriate actions.
Delivery Checklist
Ensure all parts and materials have been delivered with the autosampler. For
this compare the shipment content with the checklist included in each
instrument box. Please report missing or damaged parts to your local Agilent
Technologies sales and service office.
Table 4
32
Autosampler Checklist
Description
Quantity
Autosampler
1
Power cable
1
User manual on Documentation CD (part of the shipment - not module
specific)
1
Accessory kit
1
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Installing the Autosampler
Unpacking the Autosampler
3
Autosampler Accessory Kit Contents
p/n
Description
G5667-68755
Accessory kit (bio-inert)
G5667-60500
PEEK/SST capillary (bio-inert) 0.17 mm ID, 400 mm long
5181-1519
CAN cable, Agilent module to module, 1 m
5063-6527
Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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3
Installing the Autosampler
Optimizing the Stack Configuration
Optimizing the Stack Configuration
If your module is part of a complete Agilent 1260 Infinity Liquid
Chromatograph, you can ensure optimum performance by installing the
following configurations. These configurations optimize the system flow path,
ensuring minimum delay volume.
34
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Installing the Autosampler
Optimizing the Stack Configuration
3
One Stack Configuration
Ensure optimum performance by installing the modules of the Agilent 1260
Infinity LC System in the following configuration (See Figure 6 on page 35 and
Figure 7 on page 36). This configuration optimizes the flow path for minimum
delay volume and minimizes the bench space required.
HdakZciXVW^cZi
KVXjjbYZ\VhhZg
Ejbe
AdXVaJhZg>ciZg[VXZ
6jidhVbeaZg
8dajbcXdbeVgibZci
9ZiZXidg
Figure 6
Recommended Stack Configuration for 1260 (Front View)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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3
Installing the Autosampler
Optimizing the Stack Configuration
GZbdiZXVWaZ
86C7jhXVWaZid
adXVajhZg^ciZg[VXZ
68edlZg
86C7jhXVWaZ
6cVad\YZiZXidg
h^\cVa
&dg'djiejih
eZgYZiZXidg
A6CidA88]ZbHiVi^dc
adXVi^dcYZeZcYhdcYZiZXidg
Figure 7
36
Recommended Stack Configuration for 1260 (Rear View)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Installing the Autosampler
Optimizing the Stack Configuration
3
Two Stack Configuration
To avoid excessive height of the stack when the autosampler thermostat is
added to the system it is recommended to form two stacks. Some users prefer
the lower height of this arrangement even without the autosampler
thermostat. A slightly longer capillary is required between the pump and
autosampler. (See Figure 8 on page 37 and Figure 9 on page 38).
>chiVciE^adi
9ZiZXidg
8dajbcXdbeVgibZci
6jidhVbeaZg
I]ZgbdhiVi[dgi]Z6AH
dei^dcVa
HdakZciXVW^cZi
9Z\VhhZgdei^dcVa
Ejbe
Figure 8
Recommended Two Stack Configuration for 1260 (Front View)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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3
Installing the Autosampler
Optimizing the Stack Configuration
A6CidXdcigdahd[ilVgZ
86C7jhXVWaZ
id>chiVciE^adi
I]ZgbdXVWaZ
dei^dcVa
68EdlZg
GZbdiZXVWaZ
68EdlZg
86C7jhXVWaZ
68EdlZg
Figure 9
38
Recommended Two Stack Configuration for 1260 (Rear View)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
3
Installing the Autosampler
Installing the Autosampler
Installing the Autosampler
Parts required
Description
Autosampler
Power cord
Hardware required
Other cables see below and section “Cable Overview” on page 156
Software required
ChemStation and/or Instant Pilot G4208A with the appropriate revisions, see Table 3 on page 28
WA R N I N G
Module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened and the module is connected to power.
➔ Make sure that it is always possible to access the power plug.
➔ Remove the power cable from the instrument before opening the cover.
➔ Do not connect the power cable to the Instrument while the covers are removed.
CAUTION
"Defective on arrival" problems
If there are signs of damage, please do not attempt to install the module. Inspection by
Agilent is required to evaluate if the instrument is in good condition or damaged.
➔ Notify your Agilent sales and service office about the damage.
➔ An Agilent service representative will inspect the instrument at your site and
initiate appropriate actions.
1 Place the Autosampler in the stack, see “Optimizing the Stack
Configuration” on page 34.
2 Ensure the power switch on the front of the module is OFF (switch stands
out).
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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3
Installing the Autosampler
Installing the Autosampler
3 Connect the power cable to the power connector at the rear of the module.
Figure 10
Rearview of Autosampler
4 Connect the CAN cable to other Agilent 1260 Infinity modules.
5 Connect the APG remote cable (optional) for non-Agilent instruments.
6 Turn on the power by pushing the button at the lower left hand side of the
module.
The power button stays pressed in and the status LED should be green.
40
NOTE
When the line power button stands out and the green light is off, the module is turned off.
NOTE
The module was shipped with default configuration settings. For changing these settings,
please refer to service manual "Setting the 8-bit configuration switch".
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Installing the Autosampler
Flow Connections to the Autosampler
3
Flow Connections to the Autosampler
Parts required
Description
System
Capillaries and tubing from Accessory Kit.
Preparations
NOTE
WA R N I N G
Autosampler is installed in system.
This procedure shows the Autosampler outside of a system. In an Agilent 1260 Infinity
Liquid Chromatograph, the Autosampler is located between a Pump (above) and the
Thermostatted Column Compartment (below), see “Optimizing the Stack Configuration” on
page 34
Toxic, flammable and hazardous solvents, samples and reagents
The handling of solvents, samples and reagents can hold health and safety risks.
➔ When working with these substances observe appropriate safety procedures (for
example by wearing goggles, safety gloves and protective clothing) as described in
the material handling and safety data sheet supplied by the vendor and follow good
laboratory practice.
➔ The amount of substances should be reduced to the minimal volume required for
the analysis.
➔ Do not operate the instrument in an explosive atmosphere.
1 Open the front cover by pressing the button on the right side of the module.
2 Install the capillary from the pump outlet into the port 1 of the injection
valve.
3 Install the capillary from the port 6 of the injection valve to the TCC.
NOTE
The Autosampler can only be operated with the front and side covers closed.
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Installing the Autosampler
Installation of stainless steel cladded PEEK capillaries
Installation of stainless steel cladded PEEK capillaries
Installation of Stainless Steel Cladded PEEK Capillaries
The Agilent 1260 Infinity Bio-inert LC System uses PEEK capillaries, which
are cladded with stainless steel. These capillaries combine the high pressure
stability of steel with the inertness of PEEK. They are used in the high
pressure flow path after sample introduction (needle seat capillary) through
the TCC/heat exchangers to the column. Such capillaries need to be installed
carefully in order to keep them tight without damaging them by
overtightening.
CAUTION
Strong force/torque will damage SST cladded PEEK capillaries
Be careful when installing stainless steel cladded PEEK capillaries.
➔ See Agilent 1260 Infinity Bio-inert Quaternary LC System Manual for correct
installation.
42
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Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
4
LAN Configuration
Setting up the module in a LAN environment
Connecting the module via LAN
44
45
This chapter provides information on connecting the autosampler to the Agilent
ChemStation PC.
Agilent Technologies
43
4
LAN Configuration
Setting up the module in a LAN environment
Setting up the module in a LAN environment
It is not recommended to connect an Agilent 1260 Infinity system via the
G5667A Autosampler. The detector is producing the most data in the stack,
followed by the pump, and it is therefore highly recommended to use either of
these modules for the LAN connection.
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Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
LAN Configuration
Connecting the module via LAN
4
Connecting the module via LAN
If the G5667A Autosampler is being operated as a standalone module or if a
connection via LAN is required regardless of above mentioned
recommendation, a G1369A LAN card has to be used. For installation and
configuration, see the G1369A documentation.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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4
46
LAN Configuration
Connecting the module via LAN
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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5
Using the Module
Preparing the Autosampler
48
Setting up the Autosampler with Agilent ChemStation
Control Settings 56
Method Parameter Settings 57
Module Configuration 61
52
Main Screens of the Autosampler with Agilent Instant Pilot (G4208A)
62
This chapter provides information on how to set up the autosampler for an
analysis and explains the basic settings.
Agilent Technologies
47
5
Using the Module
Preparing the Autosampler
Preparing the Autosampler
Preparing the Autosampler
For best performance of the autosampler
• When using the Autosampler in a system with a vacuum degassing unit,
shortly degas your samples before using them in the autosampler.
• Filter samples before use in 1260 system. Use High pressure filter kit (p/n
5067-4638) for inline filtering.
• When using buffer solutions, flush the system with water before switching
it off.
• Check the autosampler plungers for scratches, grooves and dents when
changing the piston seal. Damaged plungers cause micro leaks and will
decrease the lifetime of the seal.
• Priming and Purging the System - When the solvents have been exchanged
or the system has been turned off for a certain time (for example,
overnight) oxygen will re-diffuse into the solvent channel. Therefore
priming and purging of the system is required before starting an
application.
Table 5
48
Choice of Priming Solvents for Different Purposes
Activity
Solvent
Comments
After an installation
Isopropanol
Best solvent to flush air out of
the system
When switching between
reverse phase and normal
phase (both times)
Isopropanol
Best solvent to flush air out of
the system
After an installation
Ethanol or methanol
Alternative to isopropanol
(second choice) if no
isopropanol is available
To clean the system when
using buffers
Bidistilled water
Best solvent to re-dissolve
buffer crystals
After a solvent change
Bidistilled water
Best solvent to re-dissolve
buffer crystals
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Using the Module
Preparing the Autosampler
5
Solvent Information
Observe the following recommendations on the use of solvents.
• Follow recommendations for avoiding the growth of algae, see pump
manuals.
• Small particles can permanently block capillaries and valves. Therefore
always filter solvents through 0.4 µm filters.
• Avoid or minimize the use of solvents, which may corrode parts in the flow
path. Consider specifications for the pH range given for different materials
like flow cells, valve materials etc. and recommendations in subsequent
sections.
Solvent information for parts of the 1260 Infinity Bio-inert LC
system
For the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies uses
highest quality materials (see “Bio-inert Materials” on page 11) in the flow
path (also referred to as wetted parts), which are widely accepted by life
scientists, as they are known for optimum inertness towards biological
samples and ensure best compatibility to common samples and solvents over a
wide pH range. Explicitly, the complete flow path is free of stainless steel and
free of other alloys containing metals like iron, nickel, cobalt, chromium,
molybdenum or copper, which can interfere with biological samples. The flow
downstream of the sample introduction does not contain metals at all.
However there are no materials, which combine suitability for versatile HPLC
instrumentation (valves, capillaries, springs, pump heads, flow cells etc.) with
complete compatibility to all possible chemicals and application conditions.
This section gives recommendations, which solvents should be preferred.
Chemicals which are known to cause issues should be avoided or exposure
should be minimized, e.g. for short term cleaning procedures. After using
potentially aggressive chemicals, the system should be flushed with
compatible standard HPLC solvents.
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Using the Module
Preparing the Autosampler
PEEK
PEEK (Polyether-Ether Ketones) combines excellent properties regarding
biocompatibility, chemical resistance, mechanical and thermal stability and is
therefore the material of choice for biochemical instrumentation. It is stable in
the specified pH range and inert to many common solvents. There is still a
number of known incompatibilities to chemicals like chloroform, methylene
chloride, THF, DMSO, strong acids (nitric acid > 10 %, sulphuric acid > 10 %,
sulfonic acids, trichloroacetic acid), halogenes or aequous halogene solutions,
phenol and derivatives (cresols, salicylic acid etc.).
When used above room temperature, it is sensitive to bases and various
organic solvents, which can cause PEEK to swell. As normal PEEK capillaries
are very sensitive to high pressure especially under such conditions, Agilent
uses stainless-steel cladded PEEK capillaries in order to keep the flow path
free of steel and to ensure pressure stability to at least 600 bar. In case of
doubt, please consult common literature about the chemical compatibility of
PEEK.
Titanium
Titanium is highly resistant to oxidizing acids (e.g. nitric, perchloric and
hypochlorous acid) over a wide range of concentrations and temperatures,
which is due to a thin oxide layer on the surface, which is stabilized by
oxidizing compounds. Reducing acids (e.g. hydrochloric, sulfuric and
phosphoric acid) can slightly corrode titanium, which increases with acid
concentration and temperature. For example the corrosion rate of 3 % HCl
(about pH 0.1) at room temperature is about 13 µm/year. At room
temperature, titanium is resistant to concentrations of about 5 % sulfuric acid
(about pH 0.3). The addition of nitric acid to hydrochloric or sulfuric acids
significantly reduces corrosion rates. Titanium is subject to corrosion in
anhydrous methanol, which can be avoided by adding a small amount of water
(about 3 %). Slight corrosion is possible with ammonia > 10 %.
Fused silica
Fused silica is inert against all common solvents and acids except hydrofluoric
acid. It is corroded by strong bases and should not be used above pH 12 at
room temperature. The corrosion of flow cell windows can negatively affect
measurement results. For a pH larger than 12, the use of flow cells with
sapphire windows is recommended.
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Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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Preparing the Autosampler
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Gold
Gold is inert against all common HPLC solvents, acids and bases within the
specified pH range. It can be corroded by complexing cyanides and
concentrated acids like aqua regia (a mixture of concentrated hydrochloric
and nitric acid).
Zirconium Oxide
Zirconium Oxide (ZrO2) is inert against almost all common acids, bases and
solvents. There are no documented incompatibilities for HPLC applications.
Platinum/Iridium
Platinum/Iridium is inert against almost all common acids, bases and solvents.
There are no documented incompatibilities for HPLC applications.
PTFE
PTFE (polytetrafluorethen, Teflon®) is inert against almost all common acids,
bases and solvents. There are no documented incompatibilities for HPLC
applications.
Sapphire, Ruby and Al2O3-based ceramics
Sapphire, Ruby and ceramics based on Al2O3 are inert against almost all
common acids, bases and solvents. There are no documented incompatibilities
for HPLC applications.
Data above were collected from external resources and are meant as a
reference. Agilent cannot guarantee for the completeness and correctness of
such information. Information can also not be generalized due to catalytic
effects of impurities like metal ions, complexing agents, oxygen etc. Most data
available refers to room temperature (typically 20 – 25 °C, 68 – 77 °F). If
corrosion is possible it usually increases at higher temperatures. In case of
doubt, additional resources should be consulted.
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Using the Module
Setting up the Autosampler with Agilent ChemStation
Setting up the Autosampler with Agilent ChemStation
The setup of the Autosampler is shown with the Agilent ChemStation B.04.02
SP1 1260. Depending on the controller (e.g. Agilent Instant Pilot, EZChrom
Elite) the screens look different. For the Instant Pilot refer to “Main Screens of
the Autosampler with Agilent Instant Pilot (G4208A)” on page 62.
NOTE
52
This section describes the autosampler settings only. For information on the Agilent
ChemStation or other 1260 Infinity modules refer to the corresponding documentation.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Using the Module
Setting up the Autosampler with Agilent ChemStation
Figure 11
5
ChemStation Method & Run Control
After successful load of the ChemStation, you should see the module as an
active item in the graphical user interface (GUI).
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Using the Module
Setting up the Autosampler with Agilent ChemStation
The Autosampler User Interface
&
(
)
Within the Autosampler user interface, there are active
areas. If you move the mouse cursor across the icons (tray,
EMF button), the cursor will change and you may click on
the icon to
• Turn on/off the autosampler (1)
• Configure the sample tray (2)
• Get the status of the EMF (Early Maintenance Feature)
(3)
• Switch injection valve to Mainpass / Bypass (4)
Instrument actuals Information
• Injection volume
• Sample location
'
A right-click into the Active Area will open a menu to
• Show the Control User Interface (special module
settings)
• Show the Method User interface (same as via menu
Instrument – Setup G1367E)
• Set Error Method
• Identify Device
• Home Arm
• Reset Sampler
• Wash Needle
• Needle Up
• Valve Mainpass / Bypass (same as click on the valve
icon)
• Switch on Tray Illumination
• Edit Well Plate Types
• Wellplate Configuration (same as click on the Tray
icon)
54
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Using the Module
Setting up the Autosampler with Agilent ChemStation
5
Module Status shows Run / Ready / Error state and “Not
Ready text” or “Error text”.
• Error (Red)
• Not ready (yellow)
• Ready (green)
• Pre run, Post run (purple)
• Run (blue)
• Idle (green)
• Offline (dark gray)
• Standby (light gray)
EMF Status shows Run / Ready / Error state and “Not
Ready text” or “Error text”.
• Offline (gray)
• Ok. No Maintenance required (green)
• EMF warning. Maintenance might be required (yellow)
• EMF warning. Maintenance required (red)
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Using the Module
Setting up the Autosampler with Agilent ChemStation
Control Settings
These settings are available via right click on the Active Area of the ALS GUI.
Missing Vessel: The handling of missing vessels can be
configured.
Illumination: Can be turned on / off
Linked Pump: To configure which pump delivers flow to
the Autosampler.
Prime Flush Pump: Priming the Needle wash flush pump.
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Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Using the Module
Setting up the Autosampler with Agilent ChemStation
5
Method Parameter Settings
These settings are available via Menu > Instrument > Setup Agilent 1260 Infinity
Autosampler or via right click on the Active area.
NOTE
The signal window in the lower part is not shown when opening the parameter settings via
right mouse on the Autosampler user interface.
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Using the Module
Setting up the Autosampler with Agilent ChemStation
Injection Mode
The settable Injection volume range is from 0.1 –
20.0 µl/min. Select to use Standard injection or Injection
with Needle wash.
Needle wash
It is possible to select between using the built in flush port
of the Autosampler or using a non-capped vial. Using
needle wash is required to obtain minimum carry-over.
Stop Time
An autosampler Stop Time can be set.
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Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Using the Module
Setting up the Autosampler with Agilent ChemStation
5
Injection Cleaning
The Injection Valve Cleaning section allows you to specify
the valve switching times at the end of overlap or sample
flush.
Times 1 ... 4 are the times when the valve switches to
bypass (for time 1) or to mainpass and bypass (for times 2,
3 and 4).The times must be specified in ascending order.
You can also switch the times to off. Between the first and
second, and second and third valve switches, a rinse is
executed using the rinse volumes specified in the Injector
Cleaning section.
Valve movements specifies the number of times that the
valve switches from mainpass to bypass at times 2, 3 and 4
in the field.The maximum value is 2; default is 1.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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Using the Module
Setting up the Autosampler with Agilent ChemStation
Injection Program
The pretreatment/injector program comprises a series of
numbered lines, each specifying an operation that the
autosampler carries out sequentially. When you activate a
pretreatment/injector program, it replaces the standard
injection cycle.
Select Append to add the contents of the edit line to the
end of the table.
Select Insert to insert the contents of the edit line above
the currently-selected line.
Select Delete to delete the currently selected line.
Select Clear All to clear all pretreatment/injector program
functions from the table.
Select Move up to move the currently selected line one
position up in the order of execution.
Select Move down to move the currently selected line one
position down in the order of execution.
Select Cut to delete the currently-selected line and place it
on the clipboard.
Select Copy to copy the currently selected line to the
clipboard.
Select Paste to paste the line on the clipboard at the
current position.
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Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Using the Module
Setting up the Autosampler with Agilent ChemStation
5
Module Configuration
These settings are available via menu Instrument > More 1260 Infinity ALS >
Autosampler Configuration.
Device name: based on the module.
Type ID: based on the module (product number). Some
modules may allow changing the type based on
hardware/firmware. This results in a change of features
and functions.
Serial number: based on the module.
Firmware revision: based on the module.
Options: lists installed options.
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Using the Module
Main Screens of the Autosampler with Agilent Instant Pilot (G4208A)
Main Screens of the Autosampler with Agilent Instant Pilot
(G4208A)
Below the main screens for the use of the autosampler are shown.
The Control screen allows
• System: On/Off
• System: Get Ready
• System: Clear Errors
• HIP ALS: Wash needle
The System Info screen lists details of the autosampler
• Firmware revision
• On-time
• Main Board information
• Transport assembly information
• Sampling unit information
• Syringe information
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Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Using the Module
Main Screens of the Autosampler with Agilent Instant Pilot (G4208A)
5
The Configure screen allows to configure
• Symbolic Name of module
• Volumes
• On Missing Vessel behaviour
• Plate configuration
• Flush-Out Pump
• Serial Interface configuration
• Sample Illumination
The Method screen lists all method parameters of the
autosampler. These can be edited.
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Using the Module
Main Screens of the Autosampler with Agilent Instant Pilot (G4208A)
The Maintenance screen allows
• EMF setup
• logging of maintenance activities
• module identification (blinking LED)
Firmware updates can be done via the System
Maintenance screen.
The Diagnosis screen provides access to module specific
tests.
• Injector steps
64
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Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
6
Optimizing Performance
Delay Volume and Extra-Column Volume
Delay Volume 66
66
How to Configure the Optimum Delay Volume
How to Achieve Higher Injection Volumes
How to Achieve High Throughput
67
69
71
How to Achieve Higher Resolution
72
How to Achieve Higher Sensitivity
75
How to Achieve Lowest Carry Over
76
This chapter gives hints on how to optimize the performance or use additional
devices.
Agilent Technologies
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Optimizing Performance
Delay Volume and Extra-Column Volume
Delay Volume and Extra-Column Volume
The delay volume is defined as the system volume between the point of mixing
in the pump and the top of the column.
The extra-column volume is defined as the volume between the injection point
and the detection point, excluding the volume in the column.
Delay Volume
In gradient separations, this volume causes a delay between the mixture
changing in the pump and that change reaching the column. The delay
depends on the flow rate and the delay volume of the system. In effect, this
means that in every HPLC system there is an additional isocratic segment in
the gradient profile at the start of every run. Usually the gradient profile is
reported in terms of the mixture settings at the pump and the delay volume
isnot quoted even though this will have an effect on the chromatography. This
effect becomes more significant at low flow rates and small column volumes
and can have a large impact on the transferability of gradient methods. It
isimportant, therefore, for fast gradient separations to have small delay
volumes, especially with narrow bore columns (e.g., 2.1 mm i.d.) as often
usedwith mass spectrometric detection.
The delay volume in a system includes the volume in the pump from the
pointof mixing, connections between pump and autosampler, volume of the
flow path through the autosampler and connections between autosampler and
column.
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How to Configure the Optimum Delay Volume
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How to Configure the Optimum Delay Volume
For very fast gradients over 0.5 min the delay volume of the system can be
easily reduced without changing the physical configuration of the system. The
change is achieved by changing the behavior of the autosampler.
The 80 µl delay volume of the autosampler is due to the flow path from the
injection valve through the metering device, needle, needle seat and
connecting capillaries back to the injection valve (see Figure 12 on page 68).
To make an injection the valve switches from mainpass to bypass so that the
metering device can draw the sample into the needle capillary. The injection is
made when the valve switches back to mainpass and the sample is flushed
onto the column. The valve remains in this position during analysis so that the
autosampler is continually flushed and hence the gradient has to flow through
this delay volume to reach the column. This can be eliminated by switching the
injection valve from mainpass to bypass after the injection has been made and
the injected sample has been flushed onto the column. In practice this can be
done a few seconds after injection and is activated by selecting the “Automatic
Delay Volume Reduction” (ADVR) function in the autosampler setup menu.
The Flush-out Factor (typically 5 times injection volume) ensures that enough
time is allowed to flush the sample out of the injector before switching to
bypass. This effectively reduces the system delay volume with 270 µL.
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Optimizing Performance
How to Configure the Optimum Delay Volume
&#KVakZ^cbV^ceVhh![adli]gdj\]
'#KVakZ^cWneVhh!YgVl^c\hVbeaZ
(#KVakZ^cWneVhh!lVh]^c\cZZYaZ
)#KVakZ^cbV^ceVhh!hVbeaZ^c_ZXiZY
Figure 12
Schematic of injection steps in 1260 Infinity Autosampler
When using ADVR it should be noted that the gradient has already started at
the pump at the instant of injection. The question should be asked whether the
gradient has already reached the autosampler, in which case a small step in
the gradient will result. This happens when the delay volume is less than the
flush-out volume and is not necessarily a problem but may be a factor to be
considered in a method transfer. With a flush-out factor of 5 and an injection
volume of 10 µl, the autosampler will allow 50 µl to pass through before
switching to bypass which, with a delay volume of 50 µl, means the gradient
just reached the injection valve. Smaller injection volumes will have no effect
but for larger injection volumes this will introduce a small step in the gradient.
The flow rate in use will also have an impact on the decision to use ADVR or
not. At 0.2 ml/min the delay time saved is 21 seconds while at 1.0 ml/min it is
4 seconds.
The ADVR function is unlikely to be suitable for applications involving
compounds which are known to cause carry-over problems.
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How to Achieve Higher Injection Volumes
6
How to Achieve Higher Injection Volumes
The standard configuration of the Agilent 1260 Infinity Autosampler includes
a variable volume sample loop for up to 20 µl injections. The metering device
can inject a maximum volume of 40 µl and the sample loop cartridge can be
exchanged to allow this. The system delay volume due to the autosampler will
increase accordingly.
Whenever a method is scaled down from a larger column to a smaller column
it is important that the method translation makes an allowance for reducing
the injection volume in proportion to the volume of the column to maintain
the performance of the method. This is to keep the volume of the injection at
the same percentage volume with respect to the column. This is particular
important if the injection solvent is stronger (more eluotropic) than the
starting mobile phase and any increase will affect the separation particularly
for early running peaks (low retention factor). In some cases it is the cause of
peak distortion and the general rule is to keep the injection solvent the same
or weaker than the starting gradient composition. This has a bearing on
whether, or by how much, the injection volume can be increased and the user
should check for signs of increased dispersion (wider or more skewed peaks
and reduced peak resolution) in trying to increase the injection size. If an
injection is made in a weak solvent then the volume can probably be increased
further because the effect will be to concentrate the analyte on the head of the
column at the start of the gradient. Conversely if the injection is in a stronger
solvent than the starting mobile phase then increased injection volume will
spread the band of analyte down the column ahead of the gradient resulting in
peak dispersion and loss of resolution.
Perhaps the main consideration in determining injection volume is the
diameter of the column as this will have a big impact on peak dispersion. Peak
heights can be higher on a narrow column than with a larger injection on a
wider column because there is less peak dispersion. With 2.1 mm i.d. columns
typical injection volumes might range up to 5 to10 µl but it is very dependent
on the chemistry of the analyte and mobile phase as discussed above. In a
gradient separation injection volumes of about 5 % of the column volume
might be achieved whilst maintaining good resolution and peak dispersion.
One way to achieve larger injections is to use a trapping column selected by a
switching valve to capture and concentrate the injection before switching it,
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Optimizing Performance
How to Achieve Higher Injection Volumes
i.e. injecting it, onto an analytical column, see Figure 13 on page 70. The valve
can be conveniently located in the Thermostatted Column Compartment.
Figure 13
70
Sample enrichment
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How to Achieve High Throughput
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How to Achieve High Throughput
The injection can be optimized for speed remembering that drawing the
sample too fast can reduce the reproducibility. Marginal gains are to be made
here as the sample volumes used tend towards the smaller end of the range in
any case. A significant portion of the injection time is the time taken with the
needle movements to and from the vial and into the flush port. These
manipulations can be performed while the previous separation is running.
This is known as "overlapped injection" and it can be easily turned on from the
autosampler setup screen in the ChemStation Control Software. The
autosampler can be told to switch the flow through the autosampler to bypass
after the injection has been made and then after, for example, 3 minutes into a
4 minutes run to start the process of aspirating the next sample and preparing
for injection. This can typically save 0.5 to 1 minute per injection.
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How to Achieve Higher Resolution
How to Achieve Higher Resolution
Increased resolution in a separation will improve the qualitative and
quantitative data analysis, allow more peaks to be separated or offer further
scope for speeding up the separation. This section considers how resolution
can be increased by examining the following points:
• Optimize selectivity
• Smaller particle-size packing
• Longer Columns
• Shallower gradients, faster flow
Resolution between two peaks is described by the resolution equation:
where
• Rs=resolution,
• N=plate count (measure of column efficiency),
• α=selectivity (between two peaks),
• k2=retention factor of second peak (formerly called capacity factor).
The term that has the most significant effect on resolution is the selectivity, α,
and practically varying this term involves changing the type of stationary
phase (C18, C8, phenyl, nitrile etc.), the mobile phase and temperature to
maximize the selectivity differences between the solutes to be separated. This
is a substantial piece of work which is best done with an automated method
development system which allows a wide range of conditions on different
columns and mobile phases to be assessed in an ordered scouting protocol.
This section considers how to get higher resolution with any chosen stationary
and mobile phases. If an automated method development system was used in
the decision on phases it is likely that short columns were used for fast
analysis in each step of the scouting.
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How to Achieve Higher Resolution
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The resolution equation shows that the next most significant term is the plate
count or efficiency, N, and this can be optimized in a number of ways. N is
inversely proportional to the particle size and directly proportional to the
length of a column and so smaller particle size and a longer column will give a
higher plate number. The pressure rises with the inverse square of the particle
size and proportionally with the length of the column. This is the reason that
the 1260 Infinity LC system was designed to go to 600 bar so that it can run
sub-two-micron particles and column length can be increased to 100 mm or
150 mm. There are even examples of 100 mm and 150 mm columns linked to
give 250 mm length. Resolution increases with the square root of N so
doubling the length of the column will increase resolution by a factor of 1.4.
What is achievable depends on the viscosity of the mobile phase as this relates
directly to the pressure. Methanol mixtures will generate more back pressure
than acetonitrile mixtures. Acetonitrile is often preferred because peak shapes
are better and narrower in addition to the lower viscosity but methanol
generally yields better selectivity (certainly for small molecules less than
about 500 Da). The viscosity can be reduced by increasing the temperature but
it should be remembered that this can change the selectivity of the separation.
Experiment will show if this leads to increase or decrease in selectivity. As
flow and pressure are increased it should be remembered that frictional
heating inside the column will increase and that can lead to slightly increased
dispersion and possibly a small selectivity change both of which could be seen
as a reduction in resolution. The latter case might be offset by reducing the
temperature of the thermostat by a few degrees and again experiment will
reveal the answer.
The van Deemter curve shows that the optimum flow rate through an STM
column is higher than for larger particles and is fairly flat as the flow rate
increases. Typical, close to optimum, flow rates for STM columns are:
2 ml/min for 4.6 mm i.d.; and 0.4 ml/min for 2.1 mm i.d. columns.
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How to Achieve Higher Resolution
In isocratic separations, increasing the retention factor, k, results in better
resolution because the solute is retained longer. In gradient separations the
retention is described by k* in the following equation:
where:
• k* = mean k value,
• tG = time length of gradient (or segment of gradient) (min),
• F = flow (ml/min),
• Vm = column delay volume,
• Δ%B = change in fraction of solvent B during the gradient,
• S = constant (ca. 4-5 for small molecules).
This shows that k and hence resolution can be increased by having a shallower
gradient (2 to 5 %/min change is a guideline), higher flow rate and a smaller
volume column. This equation also shows how to speed up an existing gradient
– if the flow is doubled but the gradient time is halved, k* remains constant
and the separation looks the same but happens in half the time. Recently
published research has shown how a shorter STM column (at temperatures
above 40 °C) can generate higher peak capacity than a longer STM column by
virtue of running it faster. (Refer to Petersson et al., J.Sep.Sci, 31, 2346-2357,
2008, Maximizing peak capacity and separation speed in liquid
chromatography).
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How to Achieve Higher Sensitivity
How to Achieve Higher Sensitivity
The sensitivity of a separation method is linked to the choice of stationary and
mobile phases as good separation with narrow peaks and a stable baseline
with minimal noise are desirable. The choice of instrument configuration will
have an effect and a major impact is the setup of the detector. This section
considers how sensitivity is affected by:
• Pump mixer volume
• Narrower columns
• Detector flow cell
• Detector parameters
In addition, the discussion on detector parameters also mentions the related
topics of selectivity and linearity.
Columns
Sensitivity is specified as a signal-to-noise ratio (S/N) and hence the need to
maximize peak height and minimize baseline noise. Any reduction in peak
dispersion will help to maintain peak height and so extra-column volume
should be minimized by use of short, narrow internal diameter, connection
capillaries and correctly installed fittings. Using smaller inner diameter
columns should result in higher peak height and is therefore ideal for
applications with limited sample amounts. If the same sample amount can be
injected on a smaller i.d. column, then the dilution due to column diameter
will be less and the sensitivity will increase. For example, decreasing the
column i.d. from 4.6 mm to 2.1 mm results in a theoretical gain in peak height
of 4.7 times due to the decreased dilution in the column. For a mass
spectrometer detector, the lower flow rates of narrow columns can result in
higher ionization efficiencies and therefore higher sensitivity.
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Optimizing Performance
How to Achieve Lowest Carry Over
How to Achieve Lowest Carry Over
Carryover is measured when residual peaks from a previous active-containing
injection appear in a subsequent blank solvent injection. There will be carry
over between active injections which may lead to erroneous results. The level
of carryover is reported as the area of the peak in the blank solution expressed
as a percentage of the area in the previous active injection. The Agilent 1260
Infinity autosampler is optimized for lowest carryover by careful design of the
flow path and use of materials in which sample adsorption is minimized. A
carryover figure of 0.002 % should be achievable even when a triple
quadrupole mass spectrometer is the detector. Operating settings of the
autosampler allow the user to set appropriate parameters to minimize
carryover in any application involving compounds liable to stick in the system.
The following functions of the autosampler can be used to minimize carryover:
• Internal needle wash
• External needle wash
• Needle seat backflush
• Injection valve cleaning
The flow path, including the inside of the needle, is continuously flushed in
normal operation, providing good elimination of carryover for most situations.
Automated delay volume reduction (ADVR) will reduce the delay volume but
will also reduce the flushing of the autosampler and should not be used with
analytes where carryover might be a problem.
The outside of the needle can be washed using a wash vial in a specific
location or the needle can be washed using the flush port. If a wash vial in a
tray location specified by the user is chosen then this vial should have no
septum and should contain a solvent suitable for washing the sample from the
needle. The septum is not used to avoid wiping contamination off the needle
on the downstream only to re-apply it on the upstroke. The needle can be
dipped into the vial multiple times. This will be effective in removing a small
degree of carryover but for more effective washing of the outside of the needle
use the flushport.
The flush port is located above and behind the needle seat and a peristaltic
pump delivers the wash solvent. It has a volume of 0.68 ml and the peristaltic
pump delivers 6 ml/min, which means the flush port volume is completely
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How to Achieve Lowest Carry Over
refilled with fresh solvent in 7 s. If the flush port is selected, the user can set
how long the outside of the needle is to be washed with fresh solvent. This may
be as low as two or three seconds in routine situations where carryover is less
of a problem and 10 to 20 s for more complete washing. It is recommended
that washing the outside of the needle in the flush port should be standard
procedure to avoid contaminating the needle seat. If the needle seat becomes
contaminated it will have to be back-flushed, by manually changing the flow
connections, to clean it. This is one of the tasks that can be automated using
the Flexible Cube module.
The flush port and its solvent delivery pump and tubing should be regularly
flushed to ensure the lowest carryover. For example, before using the system
each day, prime the flush pump for three minutes with appropriate solvent.
When other measures have failed to eliminate carryover it might be that
analyte is sticking inside the injector valve. The injector valve can be set to
make additional switching movements to clean out the flow path in the valve if
problems occur here with carryover. If the problem compounds need a high
percentage of organic phase for elution, it is recommended to switch the
injection valve at the high percentage of organic phase after the last peak has
eluted. It is also recommended to switch the injection valve again after the
initial conditions for the mobile phase have stabilized. This ensures that the
bypass groove in the rotor seal of the valve contains the gradient start
conditions, which is especially important for flow rates below 0.5 ml/min.
For samples where the outside of the needle cannot be cleaned sufficiently
with water or alcohol from the flush pump use wash vials with an appropriate
solvent. With an injector program several wash vials can be used for cleaning.
The optimum carry-over performance of the Autosampler is achieved after a
run-in period of new instruments or after the exchange of consumable parts
(like needle, needle seat and valve parts). During injections in this period,
surfaces of these parts adjust to each other. After this period, we recommend
back-flushing the needle seat in order to get the sealing areas between needle
and needle seat clean. Regular Preventive Maintenance service is
recommended as the carry-over performance of the Autosampler depends on
the integrity of these consumable parts. Using the G4227A Flexible Cube will
additionally improve the carry-over performance and life time of these parts.
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Troubleshooting and Diagnostics
Overview of the Module’s Indicators and Test Functions
80
Status Indicators 81
Power Supply Indicator 81
Module Status Indicator 82
User Interfaces
83
Agilent Diagnostic Software
84
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
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Troubleshooting and Diagnostics
Overview of the Module’s Indicators and Test Functions
Overview of the Module’s Indicators and Test Functions
Status Indicators
The module is provided with two status indicators which indicate the
operational state (prerun, run, and error states) of the module. The status
indicators provide a quick visual check of the operation of the module.
Error Messages
In the event of an electronic, mechanical or hydraulic failure, the module
generates an error message in the user interface. For each message, a short
description of the failure, a list of probable causes of the problem, and a list of
suggested actions to fix the problem are provided (see chapter Error
Information).
Test Functions
A series of test functions are available for troubleshooting and operational
verification after exchanging internal components (see Tests and
Calibrations).
Diagnostic Signals
The module has several signals (internal temperatures, voltages and currents
of lamps) that can be used for diagnosing baseline problems; see Diagnostic
Signals.
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Status Indicators
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Status Indicators
Two status indicators are located on the front of the module. The lower left
indicates the power supply status, the upper right indicates the module status.
Figure 14
Location of Status Indicators
Power Supply Indicator
The power supply indicator is integrated into the main power switch. When
the indicator is illuminated (green) the power is ON.
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Troubleshooting and Diagnostics
Status Indicators
Module Status Indicator
The module status indicator indicates one of six possible module conditions:
• When the status indicator is OFF (and power switch light is on), the module
is in a prerun condition, and is ready to begin an analysis.
• A green status indicator, indicates the module is performing an analysis
(run mode).
• A yellow indicator indicates a not-ready condition. The module is in a
not-ready state when it is waiting for a specific condition to be reached or
completed (for example, immediately after changing a set point), or while a
self-test procedure is running.
• An error condition is indicated when the status indicator is red. An error
condition indicates the module has detected an internal problem which
affects correct operation of the module. Usually, an error condition requires
attention (e.g. leak, defective internal components). An error condition
always interrupts the analysis.
• A red-blinking (modules with on-board LAN) or yellow-blinking (modules
without on-board LAN) indicator indicates that the module is in resident
mode (e.g. during update of main firmware).
• A fast red-blinking (modules with on-board LAN) or fast yellow-blinking
(modules without on-board LAN) indicator indicates that the module is in
boot loader mode (e.g. during update of main firmware). In such a case try
to re-boot the module or try a cold-start.
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Troubleshooting and Diagnostics
User Interfaces
User Interfaces
• Depending on the user interface, the available tests and the screens/reports
may vary.
• Preferred tool should be the Agilent Diagnostic Software, see “Agilent Lab
Advisor software.
• The Agilent ChemStation B.04.02 and above do not include any
maintenance/test functions.
• Screenshots used within these procedures are based on the Agilent Lab
Advisor Software.
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Troubleshooting and Diagnostics
Agilent Diagnostic Software
Agilent Diagnostic Software
The Agilent Lab Advisor software is a standalone product that can be used
with or without data system. Agilent Lab Advisor software helps to manage
the lab for high quality chromatographic results and can monitor in real time a
single Agilent LC or all the Agilent GCs and LCs configured on the lab
intranet.
Agilent Lab Advisor software provides diagnostic capabilities for all Agilent
1200 Infinity Series modules. This includes diagnostic capabilities, calibration
procedures and maintenance routines for all the maintenance routines.
The Agilent Lab Advisor software also allows users to monitor the status of
their LC instruments. The Early Maintenance Feedback (EMF) feature helps to
carry out preventive maintenance. In addition, users can generate a status
report for each individual LC instrument. The tests and diagnostic features as
provided by the Agilent Lab Advisor software may differ from the descriptions
in this manual. For details refer to the Agilent Lab Advisor software help files.
This manual provides lists with the names of Error Messages, Not Ready
messages, and other common issues.
The Instrument Utilities is a basic version of the LabAdvisor with limited
functionality required for installation, use and maintenance. No advanced
repair, troubleshooting and monitoring functionality is included.
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Error Information
What are Error Messages
87
General Error Messages 88
Timeout 88
Shut-Down 89
Remote Timeout 89
Synchronization Lost 90
Leak 90
Leak Sensor Open 91
Leak Sensor Short 91
Compensation Sensor Open
Compensation Sensor Short
Fan Failed 93
92
92
Module Error Messages 94
Exhaust Fan Failed 94
Front Door Error 94
Side Door Error 95
Arm Movement Failed or Arm Movement Timeout
Valve to Bypass Failed 96
Valve to Mainpass Failed 96
Needle Lock Failed 97
Needle to Needle Seat Position 97
Needle Carrier Failed 98
Missing Vial or Missing Wash Vial 98
Initialization Failed 99
Metering Home Failed 100
Motor Temperature 100
Invalid Vial Position 101
Peristaltic Pump Error 102
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Error Information
Agilent Diagnostic Software
Vessel or Wash Vessel Error 102
Vessel Stuck to Needle 103
Rear Blind Seat Missing 103
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
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Error Information
What are Error Messages
What are Error Messages
Error messages are displayed in the user interface when an electronic,
mechanical, or hydraulic (flow path) failure occurs which requires attention
before the analysis can be continued (for example, repair, or exchange of
consumables is necessary). In the event of such a failure, the red status
indicator at the front of the module is switched on, and an entry is written
into the module logbook.
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Error Information
General Error Messages
General Error Messages
General error messages are generic to all Agilent series HPLC modules and
may show up on other modules as well.
Timeout
The timeout threshold was exceeded.
Probable cause
Suggested actions
1 The analysis was completed successfully,
Check the logbook for the occurrence and
source of a not-ready condition. Restart the
analysis where required.
and the timeout function switched off the
module as requested.
2 A not-ready condition was present during a
sequence or multiple-injection run for a
period longer than the timeout threshold.
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Check the logbook for the occurrence and
source of a not-ready condition. Restart the
analysis where required.
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Error Information
General Error Messages
Shut-Down
An external instrument has generated a shut-down signal on the remote line.
The module continually monitors the remote input connectors for status
signals. A LOW signal input on pin 4 of the remote connector generates the
error message.
Probable cause
Suggested actions
1 Leak detected in another module with a CAN Fix the leak in the external instrument before
connection to the system.
restarting the module.
2 Leak detected in an external instrument with Fix the leak in the external instrument before
a remote connection to the system.
3 Shut-down in an external instrument with a
remote connection to the system.
restarting the module.
Check external instruments for a shut-down
condition.
Remote Timeout
A not-ready condition is still present on the remote input. When an analysis is
started, the system expects all not-ready conditions (for example, a not-ready
condition during detector balance) to switch to run conditions within one
minute of starting the analysis. If a not-ready condition is still present on the
remote line after one minute the error message is generated.
Probable cause
Suggested actions
1 Not-ready condition in one of the
Ensure the instrument showing the not-ready
condition is installed correctly, and is set up
correctly for analysis.
instruments connected to the remote line.
2 Defective remote cable.
Exchange the remote cable.
3 Defective components in the instrument
Check the instrument for defects (refer to the
instrument’s documentation).
showing the not-ready condition.
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Error Information
General Error Messages
Synchronization Lost
During an analysis, the internal synchronization or communication between
one or more of the modules in the system has failed.
The system processors continually monitor the system configuration. If one or
more of the modules is no longer recognized as being connected to the system,
the error message is generated.
Probable cause
Suggested actions
1 CAN cable disconnected.
•
Ensure all the CAN cables are connected
correctly.
•
Ensure all CAN cables are installed correctly.
2 Defective CAN cable.
Exchange the CAN cable.
3 Defective main board in another module.
Switch off the system. Restart the system, and
determine which module or modules are not
recognized by the system.
Leak
A leak was detected in the module.
The signals from the two temperature sensors (leak sensor and
board-mounted temperature-compensation sensor) are used by the leak
algorithm to determine whether a leak is present. When a leak occurs, the leak
sensor is cooled by the solvent. This changes the resistance of the leak sensor
which is sensed by the leak-sensor circuit on the main board.
90
Probable cause
Suggested actions
1 Loose fittings.
Ensure all fittings are tight.
2 Broken capillary.
Exchange defective capillaries.
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Error Information
General Error Messages
8
Leak Sensor Open
The leak sensor in the module has failed (open circuit).
The current through the leak sensor is dependent on temperature. A leak is
detected when solvent cools the leak sensor, causing the leak-sensor current to
change within defined limits. If the current falls outside the lower limit, the
error message is generated.
Probable cause
Suggested actions
1 Leak sensor not connected to the main
Please contact your Agilent service
representative.
board.
2 Defective leak sensor.
Please contact your Agilent service
representative.
3 Leak sensor incorrectly routed, being
Please contact your Agilent service
representative.
pinched by a metal component.
Leak Sensor Short
The leak sensor in the module has failed (short circuit).
The current through the leak sensor is dependent on temperature. A leak is
detected when solvent cools the leak sensor, causing the leak-sensor current to
change within defined limits. If the current increases above the upper limit,
the error message is generated.
Probable cause
Suggested actions
1 Defective flow sensor.
Please contact your Agilent service
representative.
2 Leak sensor incorrectly routed, being
Please contact your Agilent service
representative.
pinched by a metal component.
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Error Information
General Error Messages
Compensation Sensor Open
The ambient-compensation sensor (NTC) on the main board in the module has
failed (open circuit).
The resistance across the temperature compensation sensor (NTC) on the
main board is dependent on ambient temperature. The change in resistance is
used by the leak circuit to compensate for ambient temperature changes. If the
resistance across the sensor increases above the upper limit, the error
message is generated.
Probable cause
Suggested actions
1 Defective main board.
Please contact your Agilent service
representative.
Compensation Sensor Short
The ambient-compensation sensor (NTC) on the main board in the module has
failed (short circuit).
The resistance across the temperature compensation sensor (NTC) on the
main board is dependent on ambient temperature. The change in resistance is
used by the leak circuit to compensate for ambient temperature changes. If the
resistance across the sensor falls below the lower limit, the error message is
generated.
92
Probable cause
Suggested actions
1 Defective main board.
Please contact your Agilent service
representative.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
8
Error Information
General Error Messages
Fan Failed
The cooling fan in the module has failed.
The hall sensor on the fan shaft is used by the main board to monitor the fan
speed. If the fan speed falls below a certain limit for a certain length of time,
the error message is generated.
Probable cause
Suggested actions
1 Fan cable disconnected.
Please contact your Agilent service
representative.
2 Defective fan.
Please contact your Agilent service
representative.
3 Defective main board.
Please contact your Agilent service
representative.
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Error Information
Module Error Messages
Module Error Messages
These errors are autosampler specific.
Exhaust Fan Failed
The exhaust fan in the module has failed.
The hall sensor on the fan shaft is used by the main board to monitor the fan
speed. If the fan speed falls below a certain value the error message is
generated and the module shuts down.
Probable cause
Suggested actions
1 Fan cable disconnected.
Please contact your Agilent service
representative.
2 Defective fan.
Please contact your Agilent service
representative.
3 Defective main board.
Please contact your Agilent service
representative.
Front Door Error
The front door and/or the SLS board are damaged.
Probable cause
Suggested actions
1 The sensor on the SLS board is defective.
Please contact your Agilent service
representative.
2 The door is bent or the magnet is
Please contact your Agilent service
representative.
misplaced/broken.
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Error Information
Module Error Messages
8
Side Door Error
The side door and/or the main board are damaged.
Probable cause
Suggested actions
1 The door is bent or the magnet is
Please contact your Agilent service representative.
misplaced/broken.
2 The sensor on the main board is
Please contact your Agilent service representative.
defective.
Arm Movement Failed or Arm Movement Timeout
The transport assembly was unable to complete a movement in one of the
axes.
The processor defines a certain time window for the successful completion of
a movement in any particular axis. The movement and position of the
transport assembly is monitored by the encoders on the stepper motors. If the
processor does not receive the correct position information from the encoders
within the time window, the error message is generated.
See figure for axes identification.
• Arm Movement 0 Failed: X-axis.
• Arm Movement 1 Failed: Z-axis.
• Arm Movement 2 Failed: Theta (needle carrier rotation).
Probable cause
Suggested actions
1 Mechanical obstruction.
Ensure unobstructed movement of the transport
assembly.
2 High friction in the transport assembly.
Please contact your Agilent service representative.
3 Defective motor assembly.
Please contact your Agilent service representative.
4 Defective sample transport assembly
Please contact your Agilent service representative.
flex board.
5 Defective main board.
Please contact your Agilent service representative.
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Error Information
Module Error Messages
Valve to Bypass Failed
The injection valve failed to switch to the bypass position.
The switching of the injection valve is monitored by two microswitches on the
valve assembly. The switches detect the successful completion of the valve
movement. If the valve fails to reach the bypass position, or if the microswitch
does not close, the error message is generated.
Probable cause
Suggested actions
1 Valve in an intermediate position between
Turn the WPS main power OFF and ON.
the By-and the Main position.
2 Defective injection valve.
Please contact your Agilent service
representative.
3 Defective main board.
Please contact your Agilent service
representative.
Valve to Mainpass Failed
The injection valve failed to switch to the mainpass position.
The switching of the injection valve is monitored by two microswitches on the
valve assembly. The switches detect the successful completion of the valve
movement. If the valve fails to reach the mainpass position, or if the
microswitch does not close, the error message is generated.
Probable cause
Suggested actions
1 Valve in an intermediate position between
Turn the sampler main power OFF and ON.
the bypass and mainpass positions.
96
2 Defective injection valve.
Please contact your Agilent service
representative.
3 Defective main board.
Please contact your Agilent service
representative.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Error Information
Module Error Messages
8
Needle Lock Failed
The lock assembly on the sampling unit failed to move successfully.
The upper and lower positions of the needle lock are monitored by position
sensors on the sampling unit flex board. The sensors detect the successful
completion of the needle lock movement. If the needle lock fails to reach the
end point, or if the sensors fail to recognize the needle lock movement, the
error message is generated.
Probable cause
Suggested actions
1 Defective or dirty position sensor. Clean the position sensor.
2 Sticking spindle assembly.
Please contact your Agilent service representative.
3 Defective needle drive motor
Please contact your Agilent service representative.
4 Defective main board.
Please contact your Agilent service representative.
Needle to Needle Seat Position
The needle failed to reach the end position in the needle seat.
The position of the needle is monitored by a position encoder on the needle
carrier. If the needle fails to reach the end point, or if the encoder fails to
recognize the needle carrier movement, the error message is generated.
Probable cause
Suggested actions
1 Bad sample transport/sampling
Do an auto-alignment
unit alignment
2 Bent needle.
Check and exchange the needle assembly if necessary.
3 Missing needle.
Clean or change the needle seat assembly if necessary.
4 Blocked seat.
Exchange the needle carrier assembly.
5 Defective position sensor in the
Please contact your Agilent service representative.
needle carrier assembly.
6 Defective 1260 Infinity board.
Please contact your Agilent service representative.
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Error Information
Module Error Messages
Needle Carrier Failed
The needle carrier on the Sample Transport Assembly failed to move correctly.
Probable cause
Suggested actions
1 Defective Z-motor.
Exchange the needle carrier assembly.
2 Vial pusher blocked.
Please contact your Agilent service
representative.
3 Bad needle carrier positioning in X or Theta.
Exchange the needle carrier assembly.
4 Defective vial pusher sensor.
Please contact your Agilent service
representative.
5 Defective 1260 Infinity main board.
Please contact your Agilent service
representative.
Missing Vial or Missing Wash Vial
No vial was found in the position defined in the method or sequence.
When the needle carrier moves to a vial and the needle goes into the vial, the
position of the needle is monitored by an encoder behind the vial pusher. If no
vial is present, the encoder detects an error and the message “missing vial” is
generated.
Probable cause
Suggested actions
1 No vial in the position defined in the method
Install the sample vial in the correct position, or
edit the method or sequence accordingly.
or sequence.
98
2 Defective needle carrier assembly.
Exchange the needle carrier assembly.
3 Defective transport assembly flex board.
Please contact your Agilent service
representative.
4 Defective 1260 Infinity board.
Please contact your Agilent service
representative.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Error Information
Module Error Messages
8
Initialization Failed
The autosampler failed to complete initialization correctly.
The autosampler initialization procedure moves the needle arm and transport
assembly to their home positions in a predefined routine. During
initialization, the processor monitors the position sensors and motor encoders
to check for correct movement. If one or more of the movements is not
successful, or is not detected, the error message is generated.
Probable cause
Suggested actions
1 Side door not installed correctly.
•
Check if the side door is installed correctly.
•
Check if the magnet is in place in the side
door.
2 Sample transport/sampling unit not aligned
Do an auto-alignment
correctly.
3 Mechanical obstruction.
Ensure unobstructed movement of the transport
assembly.
4 Defective sampling unit flex board.
Please contact your Agilent service
representative.
5 Defective transport assembly flex board.
Please contact your Agilent service
representative.
6 Defective sampling unit motor.
Please contact your Agilent service
representative.
7 Defective 1260 Infinity main board.
Please contact your Agilent service
representative.
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Error Information
Module Error Messages
Metering Home Failed
The metering plunger has failed to move back to the home position.
The home position sensor on the sampling unit flex board monitors the home
position of the plunger. If the plunger fails to move to the home position, or if
the sensor fails to recognize the plunger position, the error message is
generated.
Probable cause
Suggested actions
1 Dirty or defective sensor.
Exchange the sampling unit flex board.
2 Broken plunger.
Exchange the metering plunger and seal.
3 Defective metering-drive motor.
Please contact your Agilent service
representative.
4 Defective 1260 Infinity board.
Please contact your Agilent service
representative.
Motor Temperature
One of the motors of the transport assembly has drawn excessive current,
causing the motor to become too hot. The processor has switched off the
motor to prevent damage to the motor.
See figure for motor identification.
• Motor 0 temperature: X-axis motor.
• Motor 2 temperature: Theta (gripper rotation) motor.
• Motor 1 temperature: Z-axis motor.
The processor monitors the current drawn by each motor and the time the
motor is drawing current. The current drawn by the motors is dependent on
the load on each motor (friction, mass of components etc.). If the current
drawn is too high, or the time the motor draws current is too long, the error
message is generated.
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Error Information
Module Error Messages
Probable cause
Suggested actions
1 Mechanical obstruction.
Ensure unobstructed movement of the transport
assembly.
2 High friction in the transport assembly.
Please contact your Agilent service
representative.
3 Motor belt tension too high.
Switch off the module at the power switch. Wait
at least 10 minutes before switching on again.
4 Defective motor.
Please contact your Agilent service
representative.
5 Defective transport assembly flex board.
Please contact your Agilent service
representative.
Invalid Vial Position
The vial position defined in the method or sequence does not exist.
The reflection sensors on the transport assembly flex board are used to check
automatically which sample trays are installed (coding on tray). If the vial
position does not exist in the current sample tray configuration, the error
message is generated.
Probable cause
Suggested actions
1 Incorrect tray installed.
Install the correct trays, or edit the method or
sequence accordingly.
2 Incorrect tray definition.
Install the correct trays, or edit the method or
sequence accordingly.
3 Incorrect vial positions defined in the
Install the correct trays, or edit the method or
sequence accordingly.
method or sequence.
4 Tray recognition defective (dirty sample tray
•
Ensure the coding surfaces of the sample
tray are clean (located at the rear of the
sample tray).
•
Please contact your Agilent service
representative.
or defective transport assembly flex board).
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Error Information
Module Error Messages
Peristaltic Pump Error
The peristaltic pump motor in the autosampler has failed.
The current on the motor is used by the MTP board to monitor the speed of the
peristaltic pump motor. If the current falls below a certain value, the error
message is generated.
Probable cause
Suggested actions
1 Defective motor.
Please contact your Agilent service
representative.
2 Defective SUD board.
Please contact your Agilent service
representative.
3 Defective 1260 Infinity main board.
Please contact your Agilent service
representative.
Vessel or Wash Vessel Error
The needle does not reach the target position in the vial or in the vessel of the
well plate.
The sensor behind the vial pusher in the needle carrier assembly detects the
successful completion of the needle movement to the vessel. If the needle fails
to reach the end point, the sensor fails to recognize the needle movement and
the error message is generated.
Probable cause
Suggested actions
1 Bad vessel definition in the plate
Check the vessel definition in the plate
configuration.
configuration.
2 Closing mat to rigid/thick.
Check that the closing mats is not too thick.
3 Bad X or Theta positioning.
Exchange the needle carrier assembly.
4 Defective encoder on the needle carrier
Please contact your Agilent service
representative.
assembly.
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Error Information
Module Error Messages
8
Vessel Stuck to Needle
The vessel sticks to the needle when the needle moves up.
Probable cause
Suggested actions
1 Closing mat to rigid/thick.
Check that the closing mat is not too thick.
2 Bad X or Theta positioning and the needle
Exchange the needle carrier assembly.
sticks into the wall between two holes.
3 Defective encoder on the needle carrier
assembly.
Please contact your Agilent service
representative.
Rear Blind Seat Missing
Rear blind seat is missing although claimed to exist by main board information
– occurs during initialization or if the blind seat location has to be used.
Probable cause
Suggested actions
1 Blind seat is missing.
Install blind seat.
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104
Error Information
Module Error Messages
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Manual
9
Test Functions and Calibration
Introduction
106
System Pressure Test 107
System Pressure Test Evaluation
Sample Transport Self Alignment
108
109
Maintenance Positions 111
Maintenance Positions 111
Change Needle 112
Change Loop Capillary 113
Arm Position 113
Change Needle Carrier 114
Change Metering Device 114
Injector Steps 115
Injector Steps 115
Step Commands 117
This chapter describes the tests for the module.
Agilent Technologies
105
9
Test Functions and Calibration
Introduction
Introduction
All tests are described based on the Agilent Lab Advisor Software B.01.04.
Other user interfaces may not provide any test or just a few.
Interface
Comment
Available Function
Agilent Instrument Utilities
Maintenance tests available
•
•
System Pressure test
Sample transport Self
Alignment
Agilent Lab Advisor
All tests are available
•
•
System Pressure test
Sample transport Self
Alignment
Agilent ChemStation
No tests available
Adding of pressure to
chromatographic signals
possible
•
•
•
Pressure
Pressure ripple
Temperature mainboard
For details on the use of the interface refer to the interface documentation.
106
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Test Functions and Calibration
System Pressure Test
9
System Pressure Test
The test determines the leak rate of the system between pump outlet valves
and a blank nut. The blank nut can be positioned at different locations in the
system before the flow cell, to determine and verify the leak rate of individual
modules and components. The test allows for setting the pressure at which the
test is performed. The leak rate of high pressure parts are not always a linear
function and therefore it is recommended to perform the test at a pressure
that correspond to the normal operating pressure of the system.
When
Parts required
Preparations
In case of a suspected leak. To verify successful execution of maintenance tasks.
#
p/n
Description
1
01080-83202
Blank nut
Solvents must be present in both channels.
1 Run the System pressure test with the recommended user interface (for
further information see Online-Help of user interface).
Figure 15
System Pressure Test – Result
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9
Test Functions and Calibration
System Pressure Test
Figure 16
System Pressure Test – Dynamic pressure input
System Pressure Test Evaluation
System Pressure Test Failed
Probable cause
Suggested actions
1 Pump leakages
Perform the Pump Head Leak test.
2 Loose or leaky fittings
Tighten the fittings or replace capillaries.
3 Autosampler leakages
Perform the Autosampler Leak test.
4 Thermostatted Column Compartment valve
Replace the TCC valve rotor seal.
leakages
NOTE
• Notice the difference between error in the test and a failed result! An error is caused by
an abnormal termination during the operation of the test, whereas a failed result
indicates that the test results were not within the specified limits.
• Often it is only a damaged blank nut (poorly shaped from over tightening) that causes
the test to fail. Before investigating any other possible sources of failure make sure that
the blank nut you are using is in a good condition and properly tightened.
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Test Functions and Calibration
Sample Transport Self Alignment
9
Sample Transport Self Alignment
The sample transport self alignment uses predefined positions on the well
plate tray to calibrate the positioning of the needle. The sample transport self
alignment is required to compensate for larger deviations in positioning the
needle carrier. The sample transport self alignment is required after
disassembling the system or when you exchange the sample transport, the
sampling unit, the tray or the MTP main board. This function is in the
calibration screen of the Lab Advisor.
When
After disassembling the module or by larger deviations in the positioning of the needle.
Parts required
#
Description
1
None
Preparations
Well plate tray needs to be installed and empty.
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9
Test Functions and Calibration
Sample Transport Self Alignment
1 Run the Transport Alignment with the recommended user interface (for
further information see Online-Help of user interface).
Figure 17
110
Sample Transport Self Alignment– Running
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9
Test Functions and Calibration
Maintenance Positions
Maintenance Positions
Maintenance Positions
Some maintenance procedures require the needle arm, metering device, and
needle carrier to be moved to specific positions to enable easy access to
components. The maintenance functions move these assemblies into the
appropriate maintenance position. In the Agilent Lab Advisor Software the
maintenance positions can be selected from the Tools icon.
When
When performing Maintenance on the module.
Parts required
#
Description
1
None
Preparations
None
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9
Test Functions and Calibration
Maintenance Positions
1 Run the Maintenance Positions with the recommended user interface (for
further information see Online-Help of user interface).
Figure 18
Maintenance Positions– Running
Change Needle
The position is positioning the needle carrier so that there is easy access for
changing needle or needle seat. The position is to the far left, and the current
to the motors are off, so that the arm can be turned while servicing the
module.
Figure 19
112
Maintenance Positions– Change Needle
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Test Functions and Calibration
Maintenance Positions
9
Change Loop Capillary
The Change Loop Capillary command positions the arm in the middle of the tray
at half height to enable easy exchange of the loop cartridge.
Figure 20
Maintenance Positions– Change Loop Capillary
Arm Position
The home position of the autosampler ensures a better access to the tray area
and for exchanging trays. When transporting the module it is highly
recommended to use the Park Arm command, in order to place the Arm in a
position for safe transport.
Figure 21
Maintenance Positions– Arm Position
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9
Test Functions and Calibration
Maintenance Positions
Change Needle Carrier
The Change Needle Carrier function moves the needle to the front of the
autosampler, enabling easy access to the needle carrier mechanism.
Figure 22
Maintenance Positions - Needle Carrier
• Start moves the needle to the front of the sample-tray area.
• End resets the autosampler after the needle carrier has been changed.
Change Metering Device
When removing the metering device is necessary (by exchanging the metering
seal for instance), the metering drive needs to be moved to a position at the far
back, in order to prevent seal and/or piston damage.
Figure 23
114
Maintenance Positions– Change Metering device
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Test Functions and Calibration
Injector Steps
9
Injector Steps
Injector Steps
Each movement of the sampling sequence can be done under manual control.
This is useful during troubleshooting, where close observation of each of the
sampling steps is required to confirm a specific failure mode or verify
successful completion of a repair. Each injector step command actually
consists of a series of individual commands that move the autosampler
components to predefined positions, enabling the specific step to be done.
When
When troubleshooting the module.
Parts required
#
Description
1
None
Preparations
None
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9
Test Functions and Calibration
Injector Steps
1 Run the Injector steps with the recommended user interface (for further
information see Online-Help of user interface).
Figure 24
116
Injector Steps– Running
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9
Test Functions and Calibration
Injector Steps
Step Commands
Table 6
Step Commands
Step
Action
Valve Bypass
Switches injection valve to the
bypass position.
Plunger Home
Moves the plunger to the home
position.
Needle Up
Lifts the needle arm to the
upper position.
Move to Location
Move the needle arm to the vial
location on the plate.
Needle into Sample
Lowers the needle into the vial.
Draw
Metering device draws the
defined injection volume.
Needle Up
Lifts the needle out of the vial.
Needle into Seat
Lowers the needle arm into the
seat.
Valve Mainpass
Switches the injection valve to
the mainpass position.
Needle Up/Mainpass
Moves needle arm to waste
position and switches the
injection valve to the mainpass
position.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Comments
Command also switches the
valve to bypass if it is not
already in that position.
Command lifts the needle, and
lowers the needle into the
sample. Command can be done
more than once (maximum
draw volume of 40/100/
4000 µL cannot be exceeded).
Use Plunger Home to reset the
metering device.
117
9
118
Test Functions and Calibration
Injector Steps
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
10
Maintenance
Introduction to Maintenance
Warnings and Cautions
121
Overview of Maintenance
Cleaning the module
120
123
124
Removing the needle assembly
125
Installing the needle assembly
128
Exchanging the Needle Seat
Replacing the Rotor seal
131
134
Removing the metering seal
138
Installing the metering seal
141
Replacing Peristaltic Pump Cartridge
Installing the Interface Board
Replacing the Module Firmware
143
146
147
This chapter describes the maintenance of the Autosampler
Agilent Technologies
119
10 Maintenance
Introduction to Maintenance
Introduction to Maintenance
Figure 25 on page 120shows the main user accessible assemblies of the
autosampler. These parts can be accessed from the front (simple repairs) and
don't require to remove the autosampler from the system stack.
BZiZg^c\YZk^XZ
AZV`ijW^c\
HVbea^c\jc^i
AddeXVe^aaVgn
IgVchedgiVhhZbWan
CZZYaZXVgg^Zg
CZZYaZ
CZZYaZ[ajh]edgi
IgVn
EdlZghl^iX]
CZZYaZhZVi
>c_ZXi^dckVakZ
CZZYaZlVh]eZg^hiVai^Xejbe
Figure 25
120
Main user accessible assemblies
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Warnings and Cautions
10
Warnings and Cautions
WA R N I N G
Electrical shock
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened.
➔ Do not remove the metal top cover of the module. No serviceable parts inside.
➔ Only certified persons are authorized to carry out repairs inside the module.
WA R N I N G
Personal injury or damage to the product
Agilent is not responsible for any damages caused, in whole or in part, by improper
use of the products, unauthorized alterations, adjustments or modifications to the
products, failure to comply with procedures in Agilent product user guides, or use of
the products in violation of applicable laws, rules or regulations.
➔ Use your Agilent products only in the manner described in the Agilent product user
guides.
WA R N I N G
Sharp metal edges
Sharp-edged parts of the equipment may cause injuries.
➔ To prevent personal injury, be careful when getting in contact with sharp metal
areas.
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121
10 Maintenance
Warnings and Cautions
WA R N I N G
Toxic, flammable and hazardous solvents, samples and reagents
The handling of solvents, samples and reagents can hold health and safety risks.
➔ When working with these substances observe appropriate safety procedures (for
example by wearing goggles, safety gloves and protective clothing) as described in
the material handling and safety data sheet supplied by the vendor, and follow good
laboratory practice.
➔ The volume of substances should be reduced to the minimum required for the
analysis.
➔ Do not operate the instrument in an explosive atmosphere.
CAUTION
Safety standards for external equipment
➔ If you connect external equipment to the instrument, make sure that you only use
accessory units tested and approved according to the safety standards appropriate
for the type of external equipment.
CAUTION
Sample degradation and contamination of the instrument
Metal parts in the flow path can interact with the bio-molecules in the sample leading
to sample degradation and contamination.
➔ For bio-inert applications, always use dedicated bio-inert parts, which can be
identified by the bio-inert symbol or other markers described in this manual.
➔ Do not mix bio-inert and non-inert modules or parts in a bio-inert system.
NOTE
122
The electronics of the autosampler will not allow operation of the autosampler when the
top cover and the top foam are removed. A safety light switch on the main board will inhibit
the operation of the fan immediately. Voltages for the other electronic components will be
turned off after 30 seconds. The status lamp will light up red and an error will be logged into
the logbook of the user interface. Always operate the autosampler with the top covers in
place.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Overview of Maintenance
10
Overview of Maintenance
The following pages describe maintenance (simple repairs) of the autosampler
that can be carried out without opening the main cover.
Table 7
Overview of Maintenance
Procedure
Typical Frequency
Change needle/needle
seat
60.000 needle into seat
Change metering seal
30.000 injections
Peristaltic pump
cartridge
3000 hours on-time
Change rotor seal
30.000 injections
Notes
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123
10 Maintenance
Cleaning the module
Cleaning the module
The module case should be kept clean. Cleaning should be done with a soft
cloth slightly dampened with water or a solution of water and mild detergent.
Do not use an excessively damp cloth as liquid could drip into the module.
WA R N I N G
Liquid dripping into the electronic compartment of your module.
Liquid in the module electronics can cause shock hazard and damage the module.
➔ Do not use an excessively damp cloth during cleaning.
➔ Drain all solvent lines before opening any fittings.
124
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Removing the needle assembly
10
Removing the needle assembly
When
When the limit in the needle into seat counter in the EMF is exceeded or when needle shows
indications of damage, blockage or leaks.
8710-0510 ¼ inch wrench
Parts required
Preparations
WA R N I N G
#
p/n
Description
1
G5667-87200
Needle assembly (bio-inert)
In order to avoid leaks, close the shutoff valves in the pump or remove tubings from solvent bottles.
Risk of injury by uncovered needle
An uncovered needle is a risk of harm to the operator.
➔ Be carefull when you remove the needle carrier assembly.
➔ Use the silicon safety tube supplied with every new needle.
NOTE
It is recommended to always exchange the needle assembly and the needle seat at the
same time to prevent premature leakage.
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10 Maintenance
Removing the needle assembly
1 In the user interface start the maintenance mode and
2 Open the front door and remove the side door.
select Change needle/seat function. In the Agilent Lab
Advisor software the Change needle/seat function can
be found in the Tools section.
3 Turn the Needle carrier 90 ° clockwise.
126
4 Flip the Leak guide open.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Removing the needle assembly
5 Attach a 5/16 inch wrench to hold the position at the
needle assembly. Use a 1/4 inch wrench to loosen the
fitting of the loop capillary.
10
6 Pinch the holder clamp, pull back and remove the loop
capillary from the needle assembly.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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10 Maintenance
Installing the needle assembly
Installing the needle assembly
When
When the limit in the needle into seat counter in the EMF is exceeded or when needle shows
indications of damage, blockage or leaks.
Tools required
p/n
Description
8710-0510
¼ inch wrench
Parts required
Preparations
WA R N I N G
#
p/n
Description
1
G5667-87200
Needle assembly (bio-inert)
In order to avoid leaks, close the shutoff valves in the pump or remove tubings from solvent bottles.
Risk of injury by uncovered needle
An uncovered needle is a risk of harm to the operator.
➔ Be carefull when you remove the needle carrier assembly.
➔ Use the silicon safety tube supplied with every new needle.
NOTE
128
It is recommended to always exchange the needle assembly and the needle seat at the
same time to prevent premature leakage.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
10
Maintenance
Installing the needle assembly
1 Push the silicon safety tube delivered with every needle
over the needle.
2 Insert the loop capillary into the needle assembly and
tighten the fitting hand tight.
3 Pinch the holder clamp and reinsert the needle assembly 4 Attach a 5/16 inch wrench to hold the position at the
into the needle carrier.
needle assembly. Use a 1/4 inch wrench to tighten the
fitting of the loop capillary.
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10 Maintenance
Installing the needle assembly
5 Close the leak guide
6 To align the needle, position the Positioning tool on the
needle socket.
7 When Rotating the tool to position the needle it has to be Check the alignment of the needle in the needle pusher of the
moved beyond the end point to get the correct position.
needle carrier by viewing from several directions to see
that it is aligned in the center of the needle pusher.
NOTE
The needle must be centered in the needle pusher as
all alignment by the Autosampler is calculated from the
needle pusher position.
8 Remove the silicon safety tube from the needle.
9 In the user interface exit the Change needle/seat
function and exit the maintenance mode. In the Lab
Advisor software the Change needle/seat function can
be found in the Tools section.
10 Re-install the side door and close the front door.
130
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Exchanging the Needle Seat
10
Exchanging the Needle Seat
When
When seat is visibly damaged, blocked or leaks.
Tools required
p/n
Description
8710-0510
¼ inch wrench
8710-0510
¼ inch wrench flat head screwdriver
Parts required
Preparations
WA R N I N G
#
p/n
Description
1
G5667-87017
Seat assembly (bio-inert), 0.17 mm ID, 100 mm
In order to avoid leaks, close the shutoff valves in the pump or remove tubings from solvent bottles.
Risk of injury by uncovered needle
An uncovered needle is a risk of harm to the operator.
➔ Be carefull when you remove the needle carrier assembly.
➔ Use the silicon safety tube supplied with every new needle.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
131
10 Maintenance
Exchanging the Needle Seat
1 In the user interface start the maintenance mode and
2 Open the front door.
select Change needle/seat function. In the Agilent Lab
Advisor software the Change needle/seat function can
be found in the Tools section.
3 Disconnect the seat capillary from the Injection valve.
4 With a Flat head screw driver carefully lift out the needle
seat from the holder.
132
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Exchanging the Needle Seat
10
Next Steps:
5 Insert the new Needle seat. Press it firmly in position.
6 In the user interface exit the Change needle/seat function and exit the maintenance mode. In the Lab Advisor software
the Change needle/seat function can be found in the Tools section.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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10 Maintenance
Replacing the Rotor seal
Replacing the Rotor seal
When
Poor injection-volume reproducibility
Leaking injection valve
Tools required
Description
Wrench 1/4 inch
Hexagonal key, 9/64 inch
Parts required
Preparations
p/n
Description
0101-1416
Rotor seal, 3 grooves, max. 600 bar
Remove front cover.
Remove the leak tubing (if necessary).
CAUTION
Damage of the stator face
The stator face is held in place by the stator head. When removing the stator head, the
stator face might fall out of the valve.
➔ When removing the stator head, ensure the stator face does not fall out of the valve.
134
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Replacing the Rotor seal
10
1 Remove all capillary fittings from the injection-valve ports. 2 Loosen each fixing bolt two turns at a time. Remove the
bolts from the head.
3 Remove the stator head, stator face and stator ring.
4 Remove the rotor seal and isolation seal.
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10 Maintenance
Replacing the Rotor seal
5 Install the new rotor seal and isolation seal. Ensure the
metal spring inside the isolation seal faces towards the
valve body.
7 Place the stator face in place on the stator head.
6 Install the stator ring with the short of the two pins facing
towards you at the 12 O’Clock position. Ensure the ring
sits flat on the valve body.
8 Install stator head and stator face. Tighten the bolts
alternately two turns at a time until the stator head is
secure.
136
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Replacing the Rotor seal
9 Reconnect the pump capillaries to the valve ports.
10
10 Slide the waste tube into the waste holder in the leak tray.
11 Install the front cover.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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10 Maintenance
Removing the metering seal
Removing the metering seal
When
When poor injection volume reproducibility or when metering device is leaking.
Tools required
p/n
Description
8710-0510
¼ inch wrench
8710-2392
4 mm Hex key
Parts required
#
p/n
Description
1
G5611-21503
Piston seal (bio-inert)
1 In the user interface start the maintenance mode and
2 Open the front door.
select Change metering device function. In the Agilent
Lab Advisor software the Change metering device
function can be found in the Tools section.
138
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
10
Maintenance
Removing the metering seal
3 Remove the two attached capillaries with a ¼ inch
wrench.
5 Pull the metering device away from the sampling unit.
4 Unscrew alternately the two fixing screws with a 4 mm
hex key.
6 Remove the two fixing screws at the base of the metering
device.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
139
10 Maintenance
Removing the metering seal
7 Remove the head body.
8 Using the piston carefully remove the metering seal.
Clean the chamber and ensure all particular matter is
removed.
140
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
10
Maintenance
Installing the metering seal
Installing the metering seal
When
When poor injection volume reproducibility or when metering device is leaking.
Tools required
p/n
Description
8710-0510
¼ inch wrench
8710-2392
4 mm Hex key
Parts required
Preparations
#
p/n
Description
1
G5611-21503
Piston seal (bio-inert)
Removing the metering seal, see “Removing the metering seal” on page 138.
1 Install the new metering seal. Press it firmly into position. 2 Reassemble the metering device. Make sure to tighten
Avoid any offset angle as it might deform the seal.
screws firmly and have the tag on the right side looking
from the front.
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141
10 Maintenance
Installing the metering seal
3 Press the piston into the seal.
4 Reinstall the metering device to the sampling unit by
tightening the two fixing screws alternately with a 4 mm
hex key.
5 Connect the two capillaries to the metering device using a Next Steps:
1/4 inch wrench.
6 Close the front door.
7 In the user interface exit the Change Metering device
function and exit the maintenance mode. In the Lab
Advisor software the Change Metering device function
can be found in the Tools section.
142
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Replacing Peristaltic Pump Cartridge
10
Replacing Peristaltic Pump Cartridge
When
Tubing blocked or broken
Parts required
NOTE
#
p/n
Description
1
5065-4445
Peristaltic pump cartridge
The peristaltic pump cartridge is a replaceable unit. The tubing inside the pump is not
replaceable.
1 Remove the corrugated leak tubing.
2 Press the two clips on the front of the peristaltic pump
cartridge.
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143
10 Maintenance
Replacing Peristaltic Pump Cartridge
3 Pull the cartridge forward off the motor shaft.
4 Disconnect the tubing leading to the wash port and the
tubing coming from the solvent bottle.
5 Connect the wash port tubing to the upper tubing of the
new cartridge (use sand paper to get a good grip on the
tubing).
144
6 Connect the tubing coming from the solvent bottle to the
lower tubing of the new cartridge.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Replacing Peristaltic Pump Cartridge
7 Push the cartridge onto the motor shaft until the clips
10
8 Reinstall the corrugated leak tubing.
click into place.
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10 Maintenance
Installing the Interface Board
Installing the Interface Board
When
At installation or when defective.
•
Parts required
CAUTION
Flat head screwdriver
#
Description
1
Interface board
Electronic boards are sensitive to electrostatic discharge (ESD) and should be handled
with care so as not to damage them. Touching electronic boards and components can
cause electrostatic discharge.
ESD can damage electronic boards and components.
➔ Be sure to hold the board by the edges and do not touch the electrical components.
Always use an ESD protection (for example, an ESD wrist strap) when handling
electronic boards and components.
1 Switch OFF the autosampler at the main power switch.
2 Disconnect cables from the interface board connectors.
3 Loosen the screws. Slide out the interface board from the autosampler.
4 Install the interface board. Secure the screws.
5 Reconnect the cables to the board connectors
HXgZlh
Figure 26
146
Exchanging the Interface Board
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Maintenance
Replacing the Module Firmware
10
Replacing the Module Firmware
When
The installation of newer firmware might be necessary
• if a newer version solves problems of older versions or
• to keep all systems on the same (validated) revision.
The installation of older firmware might be necessary
• to keep all systems on the same (validated) revision or
• if a new module with newer firmware is added to a system or
• if third part control software requires a special version.
Tools required
Description
OR
Agilent Lab Advisor software
OR
Instant Pilot G4208A
Parts required
#
Description
1
Firmware, tools and documentation from Agilent web site
LAN/RS-232 Firmware Update Tool
Preparations
Read update documentation provided with the Firmware Update Tool.
To upgrade/downgrade the module’s firmware carry out the following
steps:
1 Download the required module firmware, the latest LAN/RS-232 FW
Update Tool and the documentation from the Agilent web.
• http://www.chem.agilent.com/scripts/cag_firmware.asp.
2 For loading the firmware into the module follow the instructions in the
documentation.
Module Specific Information
There is no specific information for this module.
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10 Maintenance
Replacing the Module Firmware
148
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
11
Parts for Maintenance
Overview of Maintenance Parts
Vial Trays
150
151
Recommended Plates and Closing Mats
Recommended Vial Plates
Accessory Kit
152
153
154
This chapter provides information on parts material required for the module.
Agilent Technologies
149
11 Parts for Maintenance
Overview of Maintenance Parts
Overview of Maintenance Parts
For bio-inert modules use bio-inert parts only!
150
Item p/n
Description
1
0101-1416
Rotor seal, 3 grooves, max. 600 bar
2
G5611-21503
Piston seal (bio-inert)
3
G5667-87200
Needle assembly (bio-inert)
4
G5667-87017
Seat assembly (bio-inert), 0.17 mm ID, 100 mm
5
G5667-60310
Loop capillary (bio-inert)
6
G5667-60003
Analytical head (bio-inert)
7
5068-0060
Bio-inert stator head
8
0100-1851
Stator face, ceramic
9
G5667-40500
Tool for needle adjustment
10
5067-4695
Sapphire piston
11
5067-4131
2 position/6port injection valve (bio-inert)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Parts for Maintenance
Vial Trays
11
Vial Trays
+
*
&
)
(
'
,
Item p/n
Description
1
G2258-60011
Tray for 2 plates + 10 x 2 mL vials
2
0515-0866
Screws for springs
3
G1313-09101
Spring
4
0570-1574
Spring stud
5
G1329-60000
Tray base
6
G1329-43200
Adapter air channel
G1367-47200
Plug channel
G4226-60021
Tray for 100 micro vials
7
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11 Parts for Maintenance
Recommended Plates and Closing Mats
Recommended Plates and Closing Mats
Table 8
Recommended plates and closing mat
Description (Part Number)
Rows
Columns
Plate height
Volume (μL)
Package
384Agilent (p/n 5042-1388)
16
24
14.4
80
30
384Corning (No Agilent PN)
16
24
14.4
80
384Nunc (No Agilent PN)
16
24
14.4
80
96 Agilent (p/n 5042-1386)
96Agilent (p/n 5042-1385)
8
12
14.3
500
10
120
96Agilent conical (p/n 5042-8502)
8
12
17.3
150
25
96CappedAgilent (p/n 5065-4402)
8
12
47.1
300
1
96Corning (No Agilent PN)
8
12
14.3
300
96CorningV (No Agilent PN)
8
12
14.3
300
96DeepAgilent31mm (p/n 5042-6454)
8
12
31.5
1000
96DeepNunc31mm (No Agilent PN)
8
12
31,5
1000
96DeepRitter41mm (No Agilent PN)
8
12
41.2
800
96Greiner (No Agilent PN)
8
12
14.3
300
96GreinerV (No Agilent PN)
8
12
14.3
250
96Nunc (No Agilent PN)
8
12
14.3
400
Closing mat for all 96 Agilent plates (p/n
5042-1389)
8
12
NOTE
152
50
50
Using vessels higher than 41 mm, will result in needle not being able to reach bottom of
vessel.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Parts for Maintenance
Recommended Vial Plates
11
Recommended Vial Plates
p/n
Description
G2255-68700
Vial plate for 54 x 2 mL vials (6/pk)
5022-6539
Vial plate for 15 x 6 mL vials (1/pk)
5022-6538
Vial plate for 27 Eppendorf tubes (1/pk)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
153
11 Parts for Maintenance
Accessory Kit
Accessory Kit
154
p/n
Description
G5667-68755
Accessory kit (bio-inert)
G5667-60500
PEEK/SST capillary (bio-inert) 0.17 mm ID, 400 mm long
5181-1519
CAN cable, Agilent module to module, 1 m
5063-6527
Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
12
Identifying Cables
Cable Overview
156
Analog Cables
158
Remote Cables
160
BCD Cables
163
CAN/LAN Cables
165
External Contact Cable
Agilent Module to PC
166
167
Agilent 1200 Module to Printer
168
This chapter provides information on cables used with the 1260 series of HPLC
modules.
Agilent Technologies
155
12 Identifying Cables
Cable Overview
Cable Overview
NOTE
Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.
Analog cables
p/n
Description
35900-60750
Agilent module to 3394/6 integrators
35900-60750
Agilent 35900A A/D converter
01046-60105
Analog cable (BNC to general purpose, spade lugs)
Remote cables
p/n
Description
03394-60600
Agilent module to 3396A Series I integrators
3396 Series II / 3395A integrator, see details in section “Remote Cables” on
page 160
03396-61010
Agilent module to 3396 Series III / 3395B integrators
5061-3378
Agilent module to Agilent 35900 A/D converters (or HP 1050/1046A/1049A)
01046-60201
Agilent module to general purpose
BCD cables
156
p/n
Description
03396-60560
Agilent module to 3396 integrators
G1351-81600
Agilent module to general purpose
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Identifying Cables
Cable Overview
12
CAN cables
p/n
Description
5181-1516
CAN cable, Agilent module to module, 0.5 m
5181-1519
CAN cable, Agilent module to module, 1 m
LAN cables
p/n
Description
5023-0203
Cross-over network cable, shielded, 3 m (for point to point connection)
5023-0202
Twisted pair network cable, shielded, 7 m (for point to point connection)
RS-232 cables
p/n
Description
G1530-60600
RS-232 cable, 2 m
RS232-61600
RS-232 cable, 2.5 m
Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not
compatible with connecting printers and plotters. It's also called "Null Modem
Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2,
4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
5181-1561
RS-232 cable, 8 m
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12 Identifying Cables
Analog Cables
Analog Cables
One end of these cables provides a BNC connector to be connected to Agilent
modules. The other end depends on the instrument to which connection is
being made.
Agilent Module to 3394/6 Integrators
p/n 35900-60750
Pin 3394/6
Pin Agilent
module
1
158
Signal Name
Not connected
2
Shield
Analog -
3
Center
Analog +
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Identifying Cables
Analog Cables
12
Agilent Module to BNC Connector
p/n 8120-1840
Pin BNC
Pin Agilent
module
Signal Name
Shield
Shield
Analog -
Center
Center
Analog +
Pin Agilent
module
Signal Name
Agilent Module to General Purpose
p/n 01046-60105
Pin
1
Not connected
2
Black
Analog -
3
Red
Analog +
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
159
12 Identifying Cables
Remote Cables
Remote Cables
One end of these cables provides a Agilent Technologies APG (Analytical
Products Group) remote connector to be connected to Agilent modules. The
other end depends on the instrument to be connected to.
Agilent Module to 3396A Integrators
p/n 03394-60600
Pin 3396A
Pin Agilent
module
Signal Name
9
1 - White
Digital ground
NC
2 - Brown
Prepare run
Low
3
3 - Gray
Start
Low
NC
4 - Blue
Shut down
Low
NC
5 - Pink
Not connected
NC
6 - Yellow
Power on
High
5,14
7 - Red
Ready
High
1
8 - Green
Stop
Low
NC
9 - Black
Start request
Low
13, 15
Active
(TTL)
Not connected
Agilent Module to 3396 Series II / 3395A Integrators
Use the cable Agilent module to 3396A Series I integrators (p/n 03394-60600)
and cut pin #5 on the integrator side. Otherwise the integrator prints START;
not ready.
160
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Identifying Cables
Remote Cables
12
Agilent Module to 3396 Series III / 3395B Integrators
p/n 03396-61010
Pin 33XX
Pin Agilent
module
Signal Name
9
1 - White
Digital ground
NC
2 - Brown
Prepare run
Low
3
3 - Gray
Start
Low
NC
4 - Blue
Shut down
Low
NC
5 - Pink
Not connected
NC
6 - Yellow
Power on
High
14
7 - Red
Ready
High
4
8 - Green
Stop
Low
NC
9 - Black
Start request
Low
13, 15
Active
(TTL)
Not connected
Agilent Module to Agilent 35900 A/D Converters
p/n 5061-3378
Pin 35900 A/D Pin Agilent
module
Signal Name
1 - White
1 - White
Digital ground
2 - Brown
2 - Brown
Prepare run
Low
3 - Gray
3 - Gray
Start
Low
4 - Blue
4 - Blue
Shut down
Low
5 - Pink
5 - Pink
Not connected
6 - Yellow
6 - Yellow
Power on
High
7 - Red
7 - Red
Ready
High
8 - Green
8 - Green
Stop
Low
9 - Black
9 - Black
Start request
Low
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Active
(TTL)
161
12 Identifying Cables
Remote Cables
Agilent Module to General Purpose
p/n 01046-60201
162
Pin Universal
Pin Agilent
module
Signal Name
Active
(TTL)
1 - White
Digital ground
2 - Brown
Prepare run
Low
3 - Gray
Start
Low
4 - Blue
Shut down
Low
5 - Pink
Not connected
6 - Yellow
Power on
High
7 - Red
Ready
High
8 - Green
Stop
Low
9 - Black
Start request
Low
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
12
Identifying Cables
BCD Cables
BCD Cables
One end of these cables provides a 15-pin BCD connector to be connected to
the Agilent modules. The other end depends on the instrument to be
connected to
Agilent Module to General Purpose
p/n G1351-81600
Wire Color
Pin Agilent
module
Signal Name
BCD Digit
Green
1
BCD 5
20
Violet
2
BCD 7
80
Blue
3
BCD 6
40
Yellow
4
BCD 4
10
Black
5
BCD 0
1
Orange
6
BCD 3
8
Red
7
BCD 2
4
Brown
8
BCD 1
2
Gray
9
Digital ground
Gray
Gray/pink
10
BCD 11
800
Red/blue
11
BCD 10
400
White/green
12
BCD 9
200
Brown/green
13
BCD 8
100
not connected
14
not connected
15
+5V
Low
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
163
12 Identifying Cables
BCD Cables
Agilent Module to 3396 Integrators
p/n 03396-60560
164
Pin 3396
Pin Agilent
module
Signal Name
BCD Digit
1
1
BCD 5
20
2
2
BCD 7
80
3
3
BCD 6
40
4
4
BCD 4
10
5
5
BCD0
1
6
6
BCD 3
8
7
7
BCD 2
4
8
8
BCD 1
2
9
9
Digital ground
NC
15
+5V
Low
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Identifying Cables
CAN/LAN Cables
12
CAN/LAN Cables
Both ends of this cable provide a modular plug to be connected to
Agilent modules CAN or LAN connectors.
CAN Cables
p/n
Description
5181-1516
CAN cable, Agilent module to module, 0.5 m
5181-1519
CAN cable, Agilent module to module, 1 m
LAN Cables
p/n
Description
5023-0203
Cross-over network cable, shielded, 3 m (for point to point connection)
5023-0202
Twisted pair network cable, shielded, 7 m (for point to point connection)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
165
12 Identifying Cables
External Contact Cable
External Contact Cable
5
10
15
1
6
11
One end of this cable provides a 15-pin plug to be connected to
Agilent modules interface board. The other end is for general purpose.
Agilent Module Interface Board to general purposes
p/n G1103-61611
166
Color
Pin Agilent
module
Signal Name
White
1
EXT 1
Brown
2
EXT 1
Green
3
EXT 2
Yellow
4
EXT 2
Grey
5
EXT 3
Pink
6
EXT 3
Blue
7
EXT 4
Red
8
EXT 4
Black
9
Not connected
Violet
10
Not connected
Grey/pink
11
Not connected
Red/blue
12
Not connected
White/green
13
Not connected
Brown/green
14
Not connected
White/yellow
15
Not connected
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Identifying Cables
Agilent Module to PC
12
Agilent Module to PC
p/n
Description
G1530-60600
RS-232 cable, 2 m
RS232-61600
RS-232 cable, 2.5 m
Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not
compatible with connecting printers and plotters. It's also called "Null Modem
Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2,
4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
5181-1561
RS-232 cable, 8 m
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
167
12 Identifying Cables
Agilent 1200 Module to Printer
Agilent 1200 Module to Printer
168
p/n
Description
5181-1529
Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics connector
on the other end (NOT FOR FW UPDATE). For use with G1323 Control Module.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
13
Hardware Information
Firmware Description
170
Interfaces 173
Overview Interfaces
175
Setting the 8-bit Configuration Switch 179
Communication Settings for RS-232C 180
Special Settings 182
Electrical Connections 183
Rear view of the module 184
Information on Instrument Serial Number
184
This chapter describes the autosampler in more detail on hardware and
electronics.
Agilent Technologies
169
13 Hardware Information
Firmware Description
Firmware Description
The firmware of the instrument consists of two independent sections:
• a non-instrument specific section, called resident system
• an instrument specific section, called main system
Resident System
This resident section of the firmware is identical for all Agilent
1100/1200/1220/1260/1290 series modules. Its properties are:
• the complete communication capabilities (CAN, LAN and RS-232C)
• memory management
• ability to update the firmware of the 'main system'
Main System
Its properties are:
• the complete communication capabilities (CAN, LAN and RS-232C)
• memory management
• ability to update the firmware of the 'resident system'
In addition the main system comprises the instrument functions that are
divided into common functions like
• run synchronization through APG remote,
• error handling,
• diagnostic functions,
• or module specific functions like
• internal events such as lamp control, filter movements,
• raw data collection and conversion to absorbance.
170
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Hardware Information
Firmware Description
13
Firmware Updates
Firmware updates can be done using your user interface:
• PC and Firmware Update Tool with local files on the hard disk
• Instant Pilot (G4208A) with files from a USB Flash Disk
• Agilent LabAdvisor software B.01.03 and above
The file naming conventions are:
PPPP_RVVV_XXX.dlb, where
PPPP is the product number, for example, 1315AB for the G1315A/B DAD,
R the firmware revision, for example, A for G1315B or B for the G1315C DAD,
VVV is the revision number, for example 102 is revision 1.02,
XXX is the build number of the firmware.
For instructions on firmware updates refer to section Replacing Firmware in
chapter "Maintenance" or use the documentation provided with the
Firmware Update Tools.
NOTE
Update of main system can be done in the resident system only. Update of the resident
system can be done in the main system only.
Main and resident firmware must be from the same set.
BV^c;LjeYViZ
BV^cHnhiZb
GZh^YZciHnhiZb
GZh^YZci;LJeYViZ
Figure 27
Firmware Update Mechanism
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
171
13 Hardware Information
Firmware Description
NOTE
Some modules are limited in downgrading due to their main board version or their initial
firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware
revision B.01.02 or to a A.xx.xx.
Some modules can be re-branded (e.g. G1314C to G1314B) to allow operation in specific
control software environments. In this case the feature set of the target type are use and
the feature set of the original are lost. After re-branding (e.g. from G1314B to G1314C), the
original feature set is available again.
All these specific informations are described in the documentation provided with the
firmware update tools.
The firmware update tools, firmware and documentation are available from
the Agilent web.
• http://www.chem.agilent.com/EN-US/SUPPORT/DOWNLOADS/FIRMWARE/
Pages/LC.aspx
172
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
13
Hardware Information
Interfaces
Interfaces
The Agilent 1200 Infinity Series modules provide the following interfaces:
Table 9
Agilent 1200 Infinity Series Interfaces
Module
CAN
LAN/BCD
(optional)
LAN
(on-board)
RS-232
Analog
APG
Remote
Special
G1310B Iso Pump
G1311B Quat Pump
G1311C Quat Pump VL
G1312B Bin Pump
G1312C Bin Pump VL
1376A Cap Pump
G2226A Nano Pump
G5611A Bio-inert Quat
Pump
2
Yes
No
Yes
1
Yes
G4220A/B Bin Pump
2
No
Yes
Yes
No
Yes
G1361A Prep Pump
2
Yes
No
Yes
No
Yes
CAN-DC- OUT for CAN
slaves
2
Yes
No
Yes
No
Yes
THERMOSTAT for
G1330B
2
G1364B FC-PS
G1364C FC-AS
G1364D FC-μS
G1367E HiP ALS
G1377A HiP micro ALS
G2258A DL ALS
G5664A Bio-inert FC-AS
G5667A Bio-inert
Autosampler
Yes
No
Yes
No
Yes
THERMOSTAT for
G1330B
CAN-DC- OUT for CAN
slaves
G4226A ALS
Yes
No
Yes
No
Yes
Pumps
Samplers
G1329B ALS
G2260A Prep ALS
2
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13 Hardware Information
Interfaces
Table 9
Agilent 1200 Infinity Series Interfaces
Module
CAN
LAN/BCD
(optional)
LAN
(on-board)
RS-232
Analog
APG
Remote
Special
G1314B VWD VL
G1314C VWD VL+
2
Yes
No
Yes
1
Yes
G1314E/F VWD
2
No
Yes
Yes
1
Yes
G4212A/B DAD
2
No
Yes
Yes
1
Yes
G1315C DAD VL+
G1365C MWD
G1315D DAD VL
G1365D MWD VL
2
No
Yes
Yes
2
Yes
G1321B FLD
G1362A RID
2
Yes
No
Yes
1
Yes
G4280A ELSD
No
No
No
Yes
Yes
Yes
EXT Contact
AUTOZERO
G1170A Valve Drive
2
No
No
No
No
No
Requires a HOST
module with on-board
LAN (e.g. G4212A or
G4220A with minimum
firmware B.06.40 or
C.06.40) or with
additional G1369C LAN
Card
G1316A/C TCC
2
No
No
Yes
No
Yes
G1322A DEG
No
No
No
No
No
Yes
AUX
G1379B DEG
No
No
No
Yes
No
No
AUX
G4227A Flex Cube
2
No
No
No
No
No
G4240A CHIP CUBE
2
Yes
No
Yes
No
Yes
Detectors
Others
174
CAN-DC- OUT for CAN
slaves
THERMOSTAT for
G1330A/B (NOT USED)
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
13
Hardware Information
Interfaces
NOTE
The detector (DAD/MWD/FLD/VWD/RID) is the preferred access point for control via
LAN. The inter-module communication is done via CAN.
• CAN connectors as interface to other modules
• LAN connector as interface to the control software
• RS-232C as interface to a computer
• REMOTE connector as interface to other Agilent products
• Analog output connector(s) for signal output
Overview Interfaces
CAN
The CAN is inter-module communication interface. It is a 2-wire serial bus
system supporting high speed data communication and real-time requirement.
LAN
The modules have either an interface slot for an LAN card (e.g. Agilent
G1369A/B LAN Interface) or they have an on-board LAN interface (e.g.
detectors G1315C/D DAD and G1365C/D MWD). This interface allows the
control of the module/system via a connected PC with the appropriate control
software.
NOTE
If an Agilent detector (DAD/MWD/FLD/VWD/RID) is in the system, the LAN should be
connected to the DAD/MWD/FLD/VWD/RID (due to higher data load). If no Agilent
detector is part of the system, the LAN interface should be installed in the pump or
autosampler.
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175
13 Hardware Information
Interfaces
RS-232C (Serial)
The RS-232C connector is used to control the module from a computer
through RS-232C connection, using the appropriate software. This connector
can be configured with the configuration switch module at the rear of the
module. Refer to Communication Settings for RS-232C.
NOTE
There is no configuration possible on main boards with on-board LAN. These are
pre-configured for
• 19200 baud,
• 8 data bit with no parity and
• one start bit and one stop bit are always used (not selectable).
The RS-232C is designed as DCE (data communication equipment) with a
9-pin male SUB-D type connector. The pins are defined as:
Table 10
RS-232C Connection Table
Pin
Direction
Function
1
In
DCD
2
In
RxD
3
Out
TxD
4
Out
DTR
5
176
Ground
6
In
DSR
7
Out
RTS
8
In
CTS
9
In
RI
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Hardware Information
Interfaces
>chigjbZci
BVaZ
Figure 28
13
E8
;ZbVaZ
;ZbVaZ BVaZ
RS-232 Cable
Analog Signal Output
The analog signal output can be distributed to a recording device. For details
refer to the description of the module’s main board.
APG Remote
The APG Remote connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features as common
shut down, prepare, and so on.
Remote control allows easy connection between single instruments or systems
to ensure coordinated analysis with simple coupling requirements.
The subminiature D connector is used. The module provides one remote
connector which is inputs/outputs (wired- or technique).
To provide maximum safety within a distributed analysis system, one line is
dedicated to SHUT DOWN the system’s critical parts in case any module detects
a serious problem. To detect whether all participating modules are switched
on or properly powered, one line is defined to summarize the POWER ON state
of all connected modules. Control of analysis is maintained by signal readiness
READY for next analysis, followed by START of run and optional STOP of run
triggered on the respective lines. In addition PREPARE and START REQUEST may
be issued. The signal levels are defined as:
• standard TTL levels (0 V is logic true, + 5.0 V is false),
• fan-out is 10,
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
177
13 Hardware Information
Interfaces
• input load is 2.2 kOhm against + 5.0 V, and
• output are open collector type, inputs/outputs (wired- or technique).
NOTE
All common TTL circuits operate with a 5 V power supply. A TTL signal is defined as "low"
or L when between 0 V and 0.8 V and "high" or H when between 2.0 V and 5.0 V (with
respect to the ground terminal).
Table 11
Remote Signal Distribution
Pin
Signal
Description
1
DGND
Digital ground
2
PREPARE
(L) Request to prepare for analysis (for example, calibration, detector
lamp on). Receiver is any module performing pre-analysis activities.
3
START
(L) Request to start run / timetable. Receiver is any module
performing run-time controlled activities.
4
SHUT DOWN
(L) System has serious problem (for example, leak: stops pump).
Receiver is any module capable to reduce safety risk.
5
Not used
6
POWER ON
(H) All modules connected to system are switched on. Receiver is any
module relying on operation of others.
7
READY
(H) System is ready for next analysis. Receiver is any sequence
controller.
8
STOP
(L) Request to reach system ready state as soon as possible (for
example, stop run, abort or finish and stop injection). Receiver is any
module performing run-time controlled activities.
9
START REQUEST
(L) Request to start injection cycle (for example, by start key on any
module). Receiver is the autosampler.
Special Interfaces
Some modules have module specific interfaces/connectors. They are described
in the module documentation.
178
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
13
Hardware Information
Setting the 8-bit Configuration Switch
Setting the 8-bit Configuration Switch
The 8-bit configuration switch is located at the rear of the module.
Modules that do not have their own LAN interface (e.g. the TCC) can be
controlled through the LAN interface of another module and a CAN
connection to that module.
Figure 29
Configuration switch (settings depend on configured mode)
All modules without on-board LAN:
• default should be ALL DIPS DOWN (= best settings)
• Bootp mode for LAN and
• 19200 baud, 8 data bit / 1 stop bit with no parity for RS-232
• DIP 1 DOWN and DIP 2 UP allows special RS-232 settings
• for boot/test modes DIPS 1+2 must be UP plus required mode
NOTE
For normal operation use the default (best) settings.
Switch settings provide configuration parameters for serial communication
protocol and instrument specific initialization procedures.
NOTE
With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed.
The preferred communication is LAN.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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13 Hardware Information
Setting the 8-bit Configuration Switch
NOTE
The following tables represent the configuration switch settings for the modules without
on-board LAN only.
Table 12
NOTE
8-bit Configuration Switch (without on-board LAN)
Mode Select
1
2
RS-232C
0
1
Reserved
1
0
TEST/BOOT
1
1
3
4
5
Baudrate
6
7
Data
Bits
8
Parity
Reserved
RSVD
SYS
RSVD
RSVD
FC
The LAN settings are done on the LAN Interface Card G1369A/B. Refer to the
documentation provided with the card.
Communication Settings for RS-232C
The communication protocol used in the column compartment supports only
hardware handshake (CTS/RTR).
Switches 1 in down and 2 in up position define that the RS-232C parameters
will be changed. Once the change has been completed, the column instrument
must be powered up again in order to store the values in the non-volatile
memory.
Table 13
Communication Settings for RS-232C Communication (without on-board LAN)
Mode
Select
1
2
RS-232C
0
1
3
4
Baudrate
5
6
Data Bits
7
8
Parity
Use the following tables for selecting the setting which you want to use for
RS-232C communication. The number 0 means that the switch is down and 1
means that the switch is up.
180
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
13
Hardware Information
Setting the 8-bit Configuration Switch
Table 14
Baudrate Settings (without on-board LAN)
Switches
Baud Rate
3
4
5
0
0
0
0
0
0
0
Table 15
Switches
Baud Rate
3
4
5
9600
1
0
0
9600
1
1200
1
0
1
14400
1
0
2400
1
1
0
19200
1
1
4800
1
1
1
38400
Data Bit Settings (without on-board LAN)
Switch 6
Data Word Size
0
7 Bit Communication
1
8 Bit Communication
Table 16
Parity Settings (without on-board LAN)
Switches
Parity
7
8
0
0
No Parity
0
1
Odd Parity
1
1
Even Parity
One start bit and one stop bit are always used (not selectable).
Per default, the module will turn into 19200 baud, 8 data bit with no parity.
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13 Hardware Information
Setting the 8-bit Configuration Switch
Special Settings
The special settings are required for specific actions (normally in a service
case).
Boot-Resident
Firmware update procedures may require this mode in case of firmware
loading errors (main firmware part).
If you use the following switch settings and power the instrument up again,
the instrument firmware stays in the resident mode. It is not operable as a
module. It only uses basic functions of the operating system for example, for
communication. In this mode the main firmware can be loaded (using update
utilities).
Table 17
Boot Resident Settings (without on-board LAN)
No LAN
Mode Select
SW1
SW2
SW3
SW4
SW5
SW6
SW7
SW8
TEST/BOOT
1
1
0
0
1
0
0
0
Forced Cold Start
A forced cold start can be used to bring the module into a defined mode with
default parameter settings.
CAUTION
Loss of data
Forced cold start erases all methods and data stored in the non-volatile memory.
Exceptions are diagnosis and repair log books which will not be erased.
➔ Save your methods and data before executing a forced cold start.
If you use the following switch settings and power the instrument up again, a
forced cold start has been completed.
Table 18
No LAN
182
Forced Cold Start Settings (without on-board LAN)
Mode Select
SW1
SW2
SW3
SW4
SW5
SW6
SW7
SW8
TEST/BOOT
1
1
0
0
1
0
0
1
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
13
Hardware Information
Electrical Connections
Electrical Connections
• The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal module data transfer and
synchronization.
• One analog output provides signals for integrators or data handling
systems.
• The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
• With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
• The power input socket accepts a line voltage of 100 – 240 VAC ± 10 % with a
line frequency of 50 or 60 Hz. Maximum power consumption varies by
module. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply.
NOTE
Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
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13 Hardware Information
Electrical Connections
Rear view of the module
Figure 30
Rear view of the module
Information on Instrument Serial Number
The serial number information on the instrument labels provide the following
information:
184
CCXZZ00000
Format
CC
Country of manufacturing
• DE = Germany
• JP = Japan
• CN = China
X
Alphabetic character A-Z (used by manufacturing)
ZZ
Alpha-numeric code 0-9, A-Z, where each combination
unambiguously denotes a module (there can be more than one
code for the same module)
00000
Serial number
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Agilent 1260 Infinity Bio-inert High Performance Autosampler User
Manual
14
Appendix
General Safety Information
Lithium Batteries Information
186
189
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 190
Radio Interference
191
Sound Emission
192
Use of Solvents
193
Agilent Technologies on Internet
194
This chapter provides addition information on safety, legal and web.
Agilent Technologies
185
14 Appendix
Electrical Connections
General Safety Information
The following general safety precautions must be observed during all phases of
operation, service, and repair of this instrument. Failure to comply with these
precautions or with specific warnings elsewhere in this manual violates safety
standards of design, manufacture, and intended use of the instrument. Agilent
Technologies assumes no liability for the customer’s failure to comply with
these requirements.
WA R N I N G
Ensure the proper usage of the equipment.
The protection provided by the equipment may be impaired.
➔ The operator of this instrument is advised to use the equipment in a manner as
specified in this manual.
Safety Standards
This is a Safety Class I instrument (provided with terminal for protective
earthing) and has been manufactured and tested according to international
safety standards.
Operation
Before applying power, comply with the installation section. Additionally the
following must be observed.
Do not remove instrument covers when operating. Before the instrument is
switched on, all protective earth terminals, extension cords,
auto-transformers, and devices connected to it must be connected to a
protective earth via a ground socket. Any interruption of the protective earth
grounding will cause a potential shock hazard that could result in serious
personal injury. Whenever it is likely that the protection has been impaired,
the instrument must be made inoperative and be secured against any intended
operation.
Make sure that only fuses with the required rated current and of the specified
type (normal blow, time delay, and so on) are used for replacement. The use of
repaired fuses and the short-circuiting of fuse holders must be avoided.
186
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Appendix
Electrical Connections
14
Some adjustments described in the manual, are made with power supplied to
the instrument, and protective covers removed. Energy available at many
points may, if contacted, result in personal injury.
Any adjustment, maintenance, and repair of the opened instrument under
voltage should be avoided whenever possible. When inevitable, this has to be
carried out by a skilled person who is aware of the hazard involved. Do not
attempt internal service or adjustment unless another person, capable of
rendering first aid and resuscitation, is present. Do not replace components
with power cable connected.
Do not operate the instrument in the presence of flammable gases or fumes.
Operation of any electrical instrument in such an environment constitutes a
definite safety hazard.
Do not install substitute parts or make any unauthorized modification to the
instrument.
Capacitors inside the instrument may still be charged, even though the
instrument has been disconnected from its source of supply. Dangerous
voltages, capable of causing serious personal injury, are present in this
instrument. Use extreme caution when handling, testing and adjusting.
When working with solvents please observe appropriate safety procedures
(e.g. goggles, safety gloves and protective clothing) as described in the material
handling and safety data sheet by the solvent vendor, especially when toxic or
hazardous solvents are used.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
187
14 Appendix
Electrical Connections
Safety Symbols
Table 19
Safety Symbols
Symbol
Description
The apparatus is marked with this symbol when the user should refer to the
instruction manual in order to protect risk of harm to the operator and to
protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected ground terminal.
Indicates eye damage may result from directly viewing the light produced
by the deuterium lamp used in this product.
The apparatus is marked with this symbol when hot surfaces are available
and the user should not touch it when heated up.
WA R N I N G
A WARNING
alerts you to situations that could cause physical injury or death.
➔ Do not proceed beyond a warning until you have fully understood and met the
indicated conditions.
CAUTION
A CAUTION
alerts you to situations that could cause loss of data, or damage of equipment.
➔ Do not proceed beyond a caution until you have fully understood and met the
indicated conditions.
188
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Appendix
Lithium Batteries Information
14
Lithium Batteries Information
WA R N I N G
Lithium batteries may not be disposed-off into the domestic waste. Transportation of
discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID,
IMDG is not allowed.
Danger of explosion if battery is incorrectly replaced.
➔ Discharged Lithium batteries shall be disposed off locally according to national
waste disposal regulations for batteries.
➔ Replace only with the same or equivalent type recommended by the equipment
manufacturer.
WA R N I N G
Lithiumbatteri - Eksplosionsfare ved fejlagtig håndtering.
Udskiftning må kun ske med batteri af samme fabrikat og type.
➔ Lever det brugte batteri tilbage til leverandøren.
WA R N I N G
Lithiumbatteri - Eksplosionsfare.
Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten.
➔ Brukt batteri returneres appararleverandoren.
NOTE
Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien
maar inleveren als KCA.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
189
14 Appendix
The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC)
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC)
Abstract
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC), adopted by EU Commission on 13 February 2003, is
introducing producer responsibility on all Electric and Electronic appliances
from 13 August 2005.
NOTE
This product complies with the WEEE Directive (2002/96/EC) marking requirements. The
affixed label indicates that you must not discard this electrical/electronic product in
domestic household waste.
Product Category: With reference to the equipment types in the WEEE Directive Annex I,
this product is classed as a “Monitoring and Control instrumentation” product.
Do not dispose off in domestic household waste
To return unwanted products, contact your local Agilent office, or see www.agilent.com for
more information.
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Appendix
Radio Interference
14
Radio Interference
Never use cables other than the ones supplied by Agilent Technologies to
ensure proper functionality and compliance with safety or EMC regulations.
Test and Measurement
If test and measurement equipment is operated with equipment unscreened
cables and/or used for measurements on open set-ups, the user has to assure
that under operating conditions the radio interference limits are still met
within the premises.
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
191
14 Appendix
Sound Emission
Sound Emission
Manufacturer’s Declaration
This statement is provided to comply with the requirements of the German
Sound Emission Directive of 18 January 1991.
This product has a sound pressure emission (at the operator position) < 70 dB.
• Sound Pressure Lp < 70 dB (A)
• At Operator Position
• Normal Operation
• According to ISO 7779:1988/EN 27779/1991 (Type Test)
192
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
14
Appendix
Sound Emission
Use of Solvents
Observe the following recommendations on the use of solvents.
• Brown glass ware can avoid growth of algae.
• Avoid the use of the following steel-corrosive solvents:
• Solutions of alkali halides and their respective acids (for example,
lithium iodide, potassium chloride, and so on),
• High concentrations of inorganic acids like sulfuric acid and nitric acid,
especially at higher temperatures (if your chromatography method
allows, replace by phosphoric acid or phosphate buffer which are less
corrosive against stainless steel),
• Halogenated solvents or mixtures which form radicals and/or acids, for
example:
2CHCl3 + O2 → 2COCl2 + 2HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the
stabilizing alcohol,
• Chromatographic grade ethers, which can contain peroxides (for
example, THF, dioxane, di-isopropylether) such ethers should be filtered
through dry aluminium oxide which adsorbs the peroxides,
• Solvents containing strong complexing agents (e.g. EDTA),
• Mixtures of carbon tetrachloride with 2-propanol or THF.
Use of Solvents with Agilent 1260 Infinity Bio-inert LC systems
Observe the following recommendations on the use of solvents with Agilent
1260 Infinity Bio-inert LC systems.
• Brown glass ware can avoid growth of algae.
• Small particles can permanently block capillaries and valves. Therefore
always filter solvents through 0.4 µm filters.
Do not use solvents or samples which are not compatible with materials in the
flow path (see in this manual "Bio-inert materials" and "Solvent information
for parts of the 1260 Infinity Bio-inert LC system".
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
193
14 Appendix
Agilent Technologies on Internet
Agilent Technologies on Internet
For the latest information on products and services visit our worldwide web
site on the Internet at:
http://www.agilent.com
Select Products/Chemical Analysis
It will provide also the latest firmware of the modules for download.
194
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Index
Index
8
C
8-bit configuration switch
without On-Board LAN
179
A
accessory kit 33, 154
Agilent Diagnostic software 84
Agilent Lab Advisor software 84
Agilent Lab Advisor 84
Agilent
on internet 194
algae 193, 193
ambient operating temperature 27
ambient non-operating temperature 27
analog signal 177
analog
cable 158
apg remote 177
arm 113
position 113
automated delay volume reduction 76
B
battery
safety information
BCD
cable 163
bench space 26
bio-inert 122
materials 11
blank nut 107
189
cable
analog 158
BCD 163
CAN 165
connecting APG remote 38
connecting CAN 38
connecting GPIB 38
connecting LAN 38
connecting the ChemStation 38
connecting the power 38
external contact 166
LAN 165
remote 160
RS-232 167
cables
analog 156
BCD 156
CAN 157
LAN 157
overview 156
remote 156
RS-232 157
CAN
cable 165
carryover 76
cautions and warnings 121
cleaning 124
Communication settings
RS-232C 180
compensation sensor open 92
compensation sensor short 92
condensation 26
configuration
one stack 35
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
two stack
37
D
defect on arrival 32
delay volume 66
delivery checklist 32
Diagnostic software 84
dimensions 27
E
electrical connections
descriptions of 183
electrostatic discharge (ESD) 146
EMF
early maintenance feedback 21
error messages
fan failed 93
rear blind seat missing 103
error messages
arm movement 95
autosampler 94
compensation sensor open 92
compensation sensor short 92
front door error 94
initialization failed 99
invalid vial position 101
leak sensor open 91
leak sensor short 91
leak 90
metering home failed 100
missing vial 98
motor temperature 100
needle lock failed 97
needle to needle seat position 97
peristaltic pump error 102
195
Index
remote timeout 89
shut-down 89
synchronization lost 90
timeout 88
valve to bypass failed 96
valve to mainpass failed 96
vessel error 102
vessel stuck to needle 103
external contact
cable 166
extra-column volume 66
F
fan failed 93
firmware
description 170
main system 170
resident system 170
update tool 171
updates 171, 147
upgrade/downgrade 147
frequency range 27
G
general error messages
88
H
humidity
internet
194
L
LAN
cable 165
leak sensor open 91
leak sensor short 91
leak 90
line frequency 27
line voltage 27
lithium batteries 189
loop capillary
change 113
maintenance
feedback 21
overview 123, 150
positions 111
removing the needle assembly
replacing firmware 147
materials
bio-inert 11
message
remote timeout 89
metering device
change 114
N
injection volume
achieving higher volumes 69
injector
steps 115
installation
bench space 26
power considerations 24
instrument layout 22
interfaces 173
196
needle carrier
change 114
needle
change 112
non-operating altitude 27
non-operating temperature 27
O
operating Altitude
27
75
72
76
P
M
27
I
operating temperature 27
optimization
achieving higher sensitivity
achieving higher resolution
achieving lowest carryover
column use 75
injection volumes 69
stack configuration 34
125
packaging
damaged 32
parts identification
cables 155
physical specifications 27
power supply indicator 81
power considerations 24
power consumption 27
power cords 25
power switch 39
principle
autosampler 15
R
remote
cable 160
repairs
cautions and warnings 121
replacing firmware 147
resolution
Optimization 72
RS-232C
cable 167
communication settings 180
S
safety class I 186
safety information
lithium batteries
189
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
Index
safety
general information 186
standards 27
symbols 188
sensitivity
optimization 75
serial number
information 184
shut-down 89
site requirements
power cords 25
solvent information 49
solvents 193, 193
special interfaces 178
specification
physical 27
stack configuration 38
rear view 38
status indicator 82
steps
injector 115
step
commands 117
synchronization lost 90
system setup and installation
optimizing stack configuration
W
warnings and cautions
weight 27
121
34
T
temperature sensor 90
test functions 80
timeout 88
troubleshooting
error messages 87, 80
status indicators 80, 81
V
vial trays 151
voltage range 27
Agilent 1260 Infinity Bio-inert High Performance Autosampler User Manual
197
www.agilent.com
In This Book
This manual contains technical information
about the Agilent 1260 Infinity Bio-inert High
Performance Autosampler G5667A.
• introduction and specifications,
• installation,
• using and optimizing,
• troubleshooting and diagnose,
• maintenance,
• parts identification,
• hardware information,
• safety and related information.
© Agilent Technologies 2011
Printed in Germany
03/2011
*G5667-90000*
*G5667-90000*
G5667-90000
Agilent Technologies

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