Quick Start Guide

Quick Start Guide

Chromeleon 7.2

7229.0004 Revision 1.0

•

July 2013

Chromeleon 7

Table of Contents

1 Introduction ............................................................. 1

1.1

About this Document ......................................................... 1

1.2

Other Documentation ........................................................ 2

2 Using Chromeleon ................................................... 3

2.1

Overview

........................................................................... 3

2.2

Starting Chromeleon

.......................................................... 4

2.3

Getting your Instrument Started

.......................................... 5

2.3.1

Making sure the Instrument Controller Service is

Running

................................................................. 5

2.3.2

Establishing a Connection ........................................ 6

2.3.3

Controlling and Monitoring the Instrument

............... 7

2.4

Creating a Sequence ........................................................... 9

2.4.1

Creating an Instrument Method

............................... 9

2.4.2

Creating a Processing Method .................................10

2.4.3

Creating a Report Template

....................................10

2.4.4

Creating a Sequence ..............................................10

2.5

Acquiring Data

..................................................................14

2.5.1

Starting an Analysis ................................................14

2.5.2

Monitoring an Ongoing Analysis

..............................16

2.6

Processing Data ................................................................17

2.6.1

Reviewing Data in the Chromatography Studio

.........17

2.6.2

Working with Chromatograms ................................19

2.6.3

Detecting and Integrating Peaks

..............................20

2.6.4

Identifying Peaks

...................................................26

2.6.5

Calibration and Quantitation

...................................30

2.7

Reviewing and Reporting Results

........................................34

2.7.1

Reviewing Results

..................................................34

2.7.2

Printing Results

.....................................................35

Quick Start Guide Page i

Chromeleon 7

2.8

MS in Chromeleon ............................................................37

2.8.1

MS Instrument Control

...........................................37

2.8.2

Acquiring Data with an MS ......................................37

2.8.3

MS Data Visualization

.............................................37

2.8.4

MS Data Processing (Processing Method) .................40

3 Appendix: Chromeleon Overview ......................... 47

3.1

Introduction

.....................................................................47

3.2

Instrument Controller Service ............................................49

3.3

Chromeleon Client

............................................................50

3.3.1

Chromeleon Console ..............................................51

3.3.2

Chromatography Studio

.........................................57

Page ii Quick Start Guide

Chromeleon 7

Copyright

Copyright © 2013 Thermo Fisher Scientific

The information contained in this document is subject to change without notice.

All rights reserved including those for photomechanical reproduction and storage on electronic media. No part of this publication may be copied or distributed, transmitted, transcribed, stored in a retrieval system, or transmitted into any human or computer language, in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties without the express written permission of Thermo Fisher Scientific Inc.

Trademarks

Windows, Windows Vista, SQL Server 2008, SQL Server, and Microsoft are registered trademarks of Microsoft Corporation.

All other trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries.

Quick Start Guide Page iii

Chromeleon 7

1 Introduction

1.1

About this Document

This Quick Start Guide provides a quick reference to help get you started with Thermo Scientific™ Dionex™ Chromeleon™. It is recommended that you review the Quick Start Guide thoroughly before working with Chromeleon.

The Quick Start Guide has two parts:

Using Chromeleon

Appendix: Chromeleon

Overview

Guidance in starting a simple analysis, processing the data, and reporting the results.

An overview of the Chromeleon interfaces and common features.

This Quick Start Guide is provided "as is". Every effort has been made to supply complete and accurate information and all technical specifications and programs have been developed with the utmost care. However, Thermo Fisher Scientific assumes no responsibility and cannot be held liable for any errors, omissions, damage, or loss that might result from any use of this manual or the information contained in it.

Quick Start Guide Page 1

Chromeleon 7

1.2

Other Documentation

Chromeleon is provided with other documents that will help you to learn more about the software. Their scope is described in the

Document Overview, which is delivered in printed form with the

Chromeleon installation media, but also available in electronic form on the installation disk in the Documents folder. The

Chromeleon Online Help is available in electronic form in the

Online Help folder.

Page 2 Quick Start Guide

Chromeleon 7

2 Using Chromeleon

2.1

Overview

Every analysis using Chromeleon follows the same 6 basic steps:

1.

Start Chromeleon

2.

Start the instrument

3.

Create a sequence

4.

Acquire data

5.

Process data

6.

Review and report results

This Quick Start Guide provides enough information to perform each of these 6 steps as part of a simple analysis. For more advanced use of Chromeleon, including performing more complex analyses, please refer to the Chromeleon Help.

Note:

It is assumed that Chromeleon has been installed and at least one instrument has been connected and configured. For details on installation, and connecting and configuring instruments, see the

Installation Guide

.


Chromeleon 7

2.2

Starting Chromeleon

To start the Chromeleon software:

• Double-click the

Chromeleon 7

icon on the desktop:

–OR–

•

Use the Windows

®

Start menu:

Select

Start

>

All Programs

>

Chromeleon 7

>

Chromeleon 7

The

Chromeleon Console

window opens. If user management is active, you will be prompted to log on to the software. Enter the credentials provided by your Chromeleon adminstrator to proceed.


Chromeleon 7

2.3

Getting your Instrument Started

To control and monitor an instrument in Chromeleon you must:

• Make sure the Instrument Controller Service is running.

•

Establish a connection.

2.3.1

Making sure the Instrument Controller Service is

Running

The Chromeleon Instrument Controller Service handles instrument communications, control, and data acquisition. It runs in the background as a Windows service on any PC to which instruments are physically connected. Its status is represented by an icon in the Notification Area of the Windows taskbar

(Figure 1).

Figure 1:

Notification Area of Windows Taskbar for Windows XP and Windows 7

Instrument Controller Service is stopped

Instrument Controller Service is running


Chromeleon 7

If the Instrument Controller Service is stopped, start it by following these steps:

1.

Right-click the Chromeleon tray icon in the notification area of the Windows taskbar.

2.

Click

Start Chromeleon Instrument Controller

.

Tip:

If the Chromeleon tray icon is not visible on the taskbar, click Start, point to Programs (or All Programs, depending on the operating system), open the Chromeleon 7 folder and click

Services Manager.

Note

: You can configure the Chromeleon Instrument

Controller Service to automatically start every time the computer is switched on. See the

Controlling Instruments and

Modules

section of the Chromeleon Help for more information.

2.3.2

Establishing a Connection

Before you can control an instrument, communication must be established between the modules and the Chromeleon

Instrument Controller. This is referred to as

Connecting the

Modules

.

To connect the modules follow these steps:

1.

In the

Console

, choose the

Instruments

category and click your instrument’s name in the

Navigation Pane

.

2.

Select the ePanel tab for the required module in the

Work

Area

(for example

Pump

).


Chromeleon 7

3.

On the

ePanel

, click

Connect

.

Note:

The ePanel sets are generated automatically to support modules from a specific instrument series (e.g., UltiMate

3000, ICS-5000). When combining modules from these instruments, ePanels for some modules may not be displayed automatically. See Managing ePanel Sets and ePanels in the

Chromeleon Help for details on manually adding ePanels to a set.

2.3.3

Controlling and Monitoring the Instrument

Usually, samples are analyzed as part of a sequence using a predefined set of instrument commands stored in an Instrument

Method. Sometimes (typically between sequence runs) it is necessary to issue individual commands to the instrument modules, such as for starting or stopping the flow, setting eluent composition, switching on or off the detector lamp, or monitoring the baseline.

To issue instrument commands follow these steps:

1.

In the

Console

, choose the

Instruments

category. Select the instrument by clicking its name in the

Navigation Pane

.

2.

In the

Work Area

select the ePanel tab for the required module.

3.

Use the controls on the

ePanel

to issue instrument commands (for example setting the wavelength of a UV/VIS detector or starting mobile phase flow on a pump).

When an analysis is running, you can monitor instrument parameters and outputs, such as the detector signal output or pump pressure, from the module’s ePanel. To monitor the detector signals without starting a sequence, click

Monitor


Chromeleon 7

Baseline

on the toolbar above the ePanel set. Click the same button again to stop monitoring the baseline. You can also save the monitor baseline data. For details see

Monitor the Baseline

in the Chromeleon Help.

Note:

Most common commands are available from the ePanels. If you need to execute a command that is not available you can access all available instrument commands from a dialog box that is accessible via the Command button

in the Instruments toolbar.

Tip:

For certain instruments, Chromeleon provides procedures for automatically starting or shutting down a chromatography system, or setting it into standby mode. These procedures are referred to as Smart Startup, Smart Shutdown, and Smart

Standby, respectively. For details of which instruments can be controlled in this way and how to use this functionality see

Smart Startup, Smart Shutdown, and Smart Standby in the

Chromeleon Help.


Chromeleon 7

2.4

Creating a Sequence

A sequence determines how a group of injections should be processed. The injection list is the primary element of the sequence. The list groups injections in the order in which they will be processed and includes injection variables (name, type, etc.) that characterize each injection.

Sequences can be created in the Console or by using an eWorkflow. eWorkflows are simple but powerful tools for creating sequences based on pre-defined templates and rules.

For more information on creating and using eWorkflows see

Creating eWorkflows


You create a sequence using the Sequence Wizard within the

Console

. But first, the following files should be prepared:

•

Instrument Method

•

Processing Method

•

Report Template

2.4.1

Creating an Instrument Method

1.

In the

Instruments

category of the

Console,

select the instrument from the Navigation Pane for which you want to create the intsrument method.

2.

On the

Create

menu, click

Instrument Method

.

3.

Complete all wizard steps and click

Finish

.

The created instrument method opens in the

Chromatography Studio.

4.

Review the instrument method by selecting the module views in the Navigation Pane. Edit settings, if required.


Chromeleon 7

5.

Save the instrument method using the

Save

icon in the

Quick Access Toolbar and specify a location and object name.

6.

Close the Chromatography Studio.

2.4.2

Creating a Processing Method

1.

In the

Console

, on the

Create

menu, click

Processing Method

.

2.

Select one of the predefined layouts and click

Next

.

3.

Enter a name, select a file location and optionally enter a comment.

4.

Click

Finish

to save the processing method and close the wizard.

The new processing method opens in the Chromatography

Studio.

2.4.3

Creating a Report Template

1.

In the

Console

, on the

Create

menu, click

Report Template

.

2.

Select one of the predefined templates and click

Next

.

3.

Select a file location and enter a file name.

4.

Click

Finish

to save the report template and close the wizard.

2.4.4

Creating a Sequence

To create a sequence using the Sequence Wizard within the

Console

:

1.

In the

Instruments

category of the

Console,

select the instrument from the Navigation Pane for which you want to create the intsrument method.


Chromeleon 7

2.

In the Console, on the

Create

menu, click

Sequence

.

3.

Complete the injection configuration settings for the injections:

Pattern for

Injection

Name:

Number of

Vials:

Injections per Vial:

Start

Position:

Injection

Volume:

Rack View

(Figure 2)

:

Specify how injections will be named.

This is a free text field to which the codes #n

#p #i and #r can be added.

(Click the button to the right of the

Pattern for Injection Name field to see definitions for these codes)

Specify the total number of vials in the analysis.

Specify the number of injections per vial in the analysis.

Specify the sequence start position of the sequence for the analysis. This specifies the position of the first vial in the autosampler.

The wizard automatically increments for the following vials.

Specify the injection volume.

If your autosampler supports the preview function, the rack view provides a graphical representation of the trays and racks that are installed on the selected instrument. The preview also allows you to conveniently determine the position of the first sample in the autosampler tray. Simply click a vial to specify the required start position. The information in the Start Position edit field is automatically updated.


Chromeleon 7

Figure 2:

Rack View

4.

Click

Next

.

Note:

When you are creating a sequence using the wizard, you can only add injections with type Unknown. After the sequence has been created, you can modify the injection types and positions.

5.

Select methods and reporting preferences: For each field use the button to navigate to and select the file you want to use. When all fields are completed click

Next

.

6.

Enter a comment for the sequence (optional) and click

Finish

.

7.

The

Save

dialog box opens.

8.

Specify where to save the sequence, enter a file name and click

Save

.


Chromeleon 7

9.

If necessary, change the injection names, injection types, injection positions, and other entries. For an overview of

injection types, refer to section 3.3.1.2.

The sequence is now visible in the Chromeleon Console’s

Navigation Pane

.


Chromeleon 7

2.5

Acquiring Data

2.5.1

Starting an Analysis

After the sequence has been created and the instrument is ready, you can start data acquisition.

2.5.1.1

Checking that the Instrument is Ready

1.

In the

Console,

choose the

Instruments

category. Select the instrument by clicking its name in the Navigation Pane.

2.

In the Work Area select the ePanel tab for the required module. To check the baseline, for example, select the detector’s ePanel and click the

Monitor Baseline

button.

When you are satisfied that the instrument is ready for analysis, start the sequence.

2.5.1.2

Starting the Sequence

1.

Select the

Data

category in the Console.

2.

In the Navigation Pane, select the sequence you want to run by clicking on it.

The Sequence then opens in the

Work Area

.

3.

Do one of the following:

• Click the down arrow on the

Start

button. Click

Add to

Queue

.

–OR–

• Click the button on the Sequence Control Bar above the injection list to add the sequence to the queue and immediately start the queue.


Chromeleon 7

The sequence is added to the queue. If the queue is empty, a

Ready Check is performed and the queue is started if there are no errors. (Figure 3).

Figure 3:

Running Sequence

Note:

The Chromeleon Queue can be used to schedule multiple sequences to run in succession. See Managing

Queues in the Chromeleon Help for information on using

Queues.

Tip:

With Chromeleon, you can automatically start or shut down a chromatography system, or set it into standby mode.

This is called Smart Startup, Smart Shutdown, and Smart

Standby. For details of the supported instruments and how to use this funtionality, see Smart Startup, Smart Shutdown, and

Smart Standby in the Chromeleon Help.


Chromeleon 7

2.5.2

Monitoring an Ongoing Analysis

During analysis it is useful to monitor the detector signal and other instrument parameters (such as pressure and temperature).

To monitor an ongoing analysis:

1.

In the

Console,

choose the

Instruments

category and click your instrument’s name in the Navigation Pane.

2.

Select the

Home

ePanel in the Work Area to monitor the detector signal and audit trail.

Note:

The settings of other modules can be monitored on their respective ePanels.


Chromeleon 7

2.6

Processing Data

After chromatographic or mass spectrometry data has been acquired, it can be processed. All processing steps are performed in the

Studio

and saved in a processing method.

To open the Studio from the Console, double-click any object in a sequence, or click the Studio button.

Chromeleon has a variety of tools to simplify or speed up data processing tasks, such as

SmartPeaks

, but only the most common processing steps are described in this Quick Start Guide.

Note:

When you make a change in the processing method, the results for all injections in the sequence which use that processing method are recalculated instantaneously. There is no need to “reprocess” the sequence or manually update results.

2.6.1

Reviewing Data in the Chromatography Studio

After data for all samples in a sequence has been acquired, it is useful to review the chromatograms and peak data before reporting the results. Often, peak detection, integration and calibration settings need to be modified before results are

reported. See chapter 2.6.2 and onwards.

1.

In the Chromeleon Console, select the

Data

category.

2.

Open the first injection of the sequence in the

Chromatography Studio by double-clicking an injection in the the Chromeleon Console’s Work Area.

The Chromatography Studio opens.


Chromeleon 7

3.

Open the

Data Processing Category

and in the Navigation

Pane select the injection you want to view.

The data for that injection is displayed in the Work Area, using the default preset view.

Tip:

There are several views in Chromeleon which can be selected from the Presets group on the Data Processing Home tab. You can also create new views and store these to be used again. See Create New View Settings in the Chromeleon Help for details on setting up custom views.

4.

You can navigate through the data by:

•

Using the buttons in the

Navigation

group of the

Data

Processing Home

tab.

• Selecting injections, channels, or components in the

Navigation Pane.

–OR–

•

Double-clicking a component or injection in the

Integration

or

Summary

table in the

Interactive Results

pane (Figure 4).


Chromeleon 7

Figure 4:

Selection in Interactive Results table

This section covers:

• Working with Chromatograms

• Detecting and Integrating Peaks

• Identifying Peaks

• Calibration and Quantitation

2.6.2

Working with Chromatograms

You can view the integrated chromatogram of the current injection in the chromatogram plot (Figure 5).

If the evaluation of data is not what you want, you can, for example, change the peak delimiters and the baseline directly in the chromatogram. Of course, you can adapt the layout of the chromatogram to your needs. It is also possible to overlay other chromatograms for comparison. For details, refer to

Working with Chromatograms



Chromeleon 7

Figure 5:

Chromatogram Plot

Tip:

You can overlay chromatograms from different injections or channels by using the pins in the Navigation Pane or by selecting the channels in the Navigation Pane (to select two or more channels, hold down CTRL or SHIFT, and then click the channels).

2.6.3

Detecting and Integrating Peaks

For correct quantification or qualification of a chromatogram, all peaks of interest must be detected and correctly integrated. To achieve this you can either:

•

Create a new set of detection parameters.

–OR–

• Modify an existing processing method

For the purposes of this guide it is assumed that the user will first create a new set of detection parameters (using the Cobra detection algorithm) and then modify these to improve the integration and peak detection.


Chromeleon 7

Note

: On the

MS Detection

tab page in the

Processing

Method

, you can select a peak detection algorithm

(Chromeleon 6, Cobra, Genesis, or ICIS) for your MS data. You can apply the peak detection settings to the entire chromatogram and/or you can define different peak detection settings for individual extracted ion chromatograms. The defined algorithm and settings apply to this extracted ion chromatogram only. For all other data than MS data, the detection algorithm can be set on the

Detection

tab page in the


.

2.6.3.1

Creating a New Set of Cobra Detection Parameters

This section gives information on how to set up a new processing method and use the

Cobra

™

Peak Detection Wizard

to determine the initial integration settings for a chromatogram.

For more information about creating processing methods, see

section 2.4.2.

1.

Open the sequence in the Chromatography Studio (by double-clicking an injection in the Console) and open the

Data Processing

category.

2.

Select the chromatogram by clicking somewhere in the chromatogram plot area.

3.

Click the

Processing

tab and activate the Cobra™ Peak

Detection Wizard by selecting the

Detection Parameters

group.

Cobra Wizard

in the

The Cobra Wizard opens.

4.

Select the area of the chromatogram to be integrated.

If the section selected contains negative peaks (for example solvent peaks), you can specify that these are ignored during


Chromeleon 7

Cobra Peak Detection by checking the

Consider Void Peak

check box. Otherwise leave this check box cleared.

Click

Next.

5.

Choose one of the following possibilities to define a range in order to determine baseline noise:

•

Select the

Use Auto Range

option (default), which calculates a suitable time range.

–OR–

• Manually enter a baseline.

Click

Next

.

6.

Choose one of the following possibilities for smoothing chromatographic data:

• Select the

Use Auto Smoothing Width

option (default) for automatically smoothing data.

–OR–

• Select the narrowest peak in the chromatogram in order to define the Cobra smoothing width used for peak detection.

Click

Next

.

7.

Choose one of the following possibilities to define the peak area threshold which determines the minimal peak area. Any peaks whose area is below this threshold will be ignored during detection or integration:

• Select the

Use Auto Minimum Area

(default) option, which automatically calculates a minimum area.

–OR–


Chromeleon 7

•

Select the smallest peak in the chromatogram that you want to be detected (the minimum area will be set to 90% of the area of the selected peak).

Click

Next.

8.

Use the drop-down

Channel

menu to select the channel, and the

Injection Type

field to specify which data the integration settings will apply to.

Click

Finish

.

9.

Click the Chromeleon icon and select

Save

>

Save

from the menu to save the updated processing method.

The

Cobra™ Peak Detection Wizard

determines the best initial integration settings for a chromatogram. For simple chromatograms, where all peaks are well resolved, these settings will be ideal. For complex chromatograms or where peaks are only partially resolved, the addition of further detection parameters may be required.

2.6.3.2

Modifying an existing Processing Method

2.6.3.2.1

Insert a New Detection Parameter

To insert a new detection parameter, follow these steps:

1.

Click in the chromatogram plot.

2.

Click the

Processing

tab.

3.

If not already shown, display the detection parameters in the chromatogram, by clicking


the


group.

in

4.

Click the arrow on the

Insert

button.


Chromeleon 7

5.

Select the appropriate detection parameter from the list.

The most frequently needed parameters are listed here. If you need other detection parameters, select

Other

Parameter.

For a list and further details on all Chromeleon detection parameters, see

Detection Parameters (Overview)

in the

Chromeleon Help.

6.

Move the cursor to the location in the chromatogram where you want to insert this parameter and click.

The new detection parameter is placed in the chromatogram and peak integration is automatically updated.

Detection parameters have a tag which shows details of the parameter and a vertical dashed line which shows the exact point of the detection parameter in the chromatogram.

2.6.3.2.2

Move, Modify and Delete Detection Parameters

1.


2.

Click the

Processing

tab.

3.

If not already shown, display the detection parameters in the chromatogram, by clicking



group. in the

4.

In the

Pointer / Zoom

group, ensure that the

Automatic

tool is selected.

To Move a Detection Parameter:

• Drag the detection parameter to the required location

(you can drag a detection parameter by its tag or line).


Chromeleon 7

To Edit the Value of a Detection Parameter:

• Double-click the tag and select a new setting from the list.

To Change the Type of Detection Parameter:

•

Right-click on the detection parameter and select

Edit

Detection Parameter

.

The

Edit Cobra Detection Parameter

dialog box opens.

•

Select a new detection parameter type from the

Parameter Name

field and set any parameter values needed.

• Click

OK

to save the change and close the

Edit Cobra

Detection Parameter

dialog box.

To Delete a Detection Parameter:

•

Right-click on the detection parameter and select

Delete


.

2.6.3.2.3

Integrate Unresolved Peaks

Partially resolved peaks can be difficult to integrate. The

SmartPeaks™ integration assistant helps you to integrate these peaks:

1.


2.

Click the

Processing

tab.

3.

On the


group, click

SmartPeaks

.

4.

Use the cursor to drag a rectangle in the chromatogram around the area that contains the partially resolved peaks.

The

SmartPeaks: Select Alternative

dialog box opens

(Figure 6).


Chromeleon 7

Figure 6:

SmartPeaks: Select Alternative dialog box

Tip:

For more options you can click the Advanced tab in the

SmartPeaks: Select Alternative dialog box. For details of how to use these settings see SmartPeaks: Select Alternative in the

Chromeleon Help.

5.

Select the preferred peak detection and click

OK

.

6.

The detection parameters in the processing method are now updated and the new detection parameters are shown in the chromatogram.

2.6.4

Identifying Peaks

After peaks have been detected and satisfactorily integrated, they can be used for reporting or results calculations. When reporting, it is useful to name components in a chromatogram.

When performing Calibration and Quantification, it is essential that at least the calibration peaks in a chromatogram are named.


Chromeleon 7

The process of assigning a name to a component is called identification. The names and expected retention times of named components are stored in a Chromeleon component table.

The following sections describe how to:

•

Create a component table.

•

Update the expected retention times and windows.

2.6.4.1

Creating a Component Table

There are three ways to create a component table:

• Via the Component Table Wizard

•

Interactively in the chromatogram pane

•

Via the component table (processing method)

2.6.4.1.1

Via the Component Table Wizard

To create a component table by using the Component Table

Wizard:

1.


2.

Click the

Processing

tab.

3.

If not already shown, display the peak windows in the chromatogram, by clicking

Component Table

group.

Peak Windows

( ) in the

4.

Click the

Component Table Wizard

button (

The Component Table Wizard dialog box opens.

).

The 5-page wizard guides you through the process of naming components. On the first 4 pages you can specify whether to


Chromeleon 7 keep existing components (adding new components to the existing ones), the time range over which to consider components, and a minimum area below which components are ignored. On the final page you can name all found components, modify the retention time and peak window, and add comments.

5.

Click

Finish

to save the component table updates and close the Component Table Wizard.

The chromatogram view in the Chromatography Studio Work

Area is automatically updated to display the new component windows.

2.6.4.1.2

Interactively in the Chromatogram Pane

To add a component from the chromatogram plot:

1.


2.

Click the

Processing

tab.

3.

Activate the

Peak Windows

option and the

Add Component

option in the

Component Table

group.

4.

Drag a rectangle around the peak you want to add to the component table.

The peak is added to the component table with a generic name (for example

Component 1

) and the peak window is visible in the chromatogram plot.

5.

Double-click the peak window tag and enter the component name.


Chromeleon 7

2.6.4.1.3

Via the Component Table (Processing Method)

To create a component table in the processing method:

1.

Click the

Data Processing Home

tab.

2.

In the

Panes

group, click


to display the processing method.

Tip:

You can maximize screen space by hiding the other panes and view the processing method only. In the Panes group, disable the panes that are not currently needed.

3.

In the processing method, select the

Component Table

tab.

4.

Click the

Click here to add a new component

link at the bottom of the component table.

A

Create new Component

dialog box opens.

5.

Type the name of the component in the

Name

field and click

Create

.

A new component is created and diplayed in the component table.

6.

Update the component retention time and window in the

Ret. Time

and/or

Window

columns of the component table.


Chromeleon 7

Tip:

Over a period of time, peak retention times may drift from their expected values. Because of this, it is sometimes useful to set wide peak windows in the component table.

When peak retention time drift is too large for this method to compensate, you can use the retention time of a previous peak for peak identification. For details on correcting for

Retention Time drift see Identify Peaks in the Chromeleon

Help.

2.6.4.2

Modifying Retention Times and Windows

You can modify the retention time and width of the peak window by modifying the properties in the component table or by dragging the peak window on the chromatogram plot.

2.6.5

Calibration and Quantitation

In order to convert the area below a peak or the peak height into absolute amount or concentration values,

calibration

is required before the analysis. The calibration coefficients obtained during calibration can then be used to calculate unknown amounts.

This section takes you through the steps required to perform a simple external standard calibration (using a single component standard at multiple concentrations). Chromeleon supports various methods of calibration including internal standards and standard addition. For information on using these methods of calibration, see

Calibration and Amount Calculation

in the

Chromeleon Help.

To perform a simple calibration in Chromeleon you need to:

• Specifiy which injections are Calibration Standards.

• Create Calibration Levels and assign these to the Calibration

Standards.


Chromeleon 7

•

Enter the amount or concentration of the Calibration

Standards.

•

Review the calibration curve.

2.6.5.1

Specifying which Injections are Calibration Standards

Calibration standards are marked as such in the injection list:

1.

Click the

Injection List

Category in the Chromeleon

Chromatography Studio window.

2.

For each Calibration Standard injection, click in the

Type

field and select

Calibration Standard

from the list of types.

2.6.5.2

Creating and Assigning Calibration Levels

1.

Click the

Injection List



2.

Click in the

Level

cell for the first calibration standard and select

Create new level

. The value 01 is added. Assign this level to all injections of this calibration standard.

3.

Repeat Step 2 for all calibration levels.

2.6.5.3

Entering Calibration Standard Amounts or

Concentrations

1.

Click the

Data Processing



2.

In the

Panes

group of the


tab, click


.

3.

In the processing method, select the

Component Table

tab

(Figure 7).


Chromeleon 7

Figure 7:

Component Table

4.

Enter the standard amount for each level of the calibration component in the appropriate cell (Level “01”, Level “02”, etc).

Calibration curves are updated automatically when you make changes to calibration standards.

2.6.5.4

Viewing Calibration Curves

You can view the calibration curve(s) (Figure 8) in the Data

Processing Category of the Chromatography Studio:

1.

Click the

Data Processing



2.

Go to the


tab.

3.

Either:

• Select a preset work area layout which contains a calibration pane from the

Presets

group.

–OR–

•

Select

Calibration Plot

in the

Panes

group (Figure 8).


Chromeleon 7

Figure 8:

A Calibration Curve

Tip:

You can view the calibration curve for each component of a multi-component standard by double-clicking the component in the calibration table, selecting the peak in the chromatogram, or by using the buttons in the Navigation group of the Data Processing Home tab.


Chromeleon 7

2.7

Reviewing and Reporting Results

After data has been acquired and calibration performed you can review and report results.

You can create report templates (which output results and other data) in any format and layout you require, or use one of

Chromeleon's default templates. This Quick Start Guide gives information on using the default templates only; for information on modifying report templates or creating new templates see

Designing Reports


To review and report results, you must first associate a template with the sequence. Once you have done this you can then:

• Review the results.

• Print results.

• Export a report.

In Chromeleon you can create an electronic report which can include worksheets comprising of chromatograms, tables, charts, calibration curves, and other instrument or results values. These electronic reports can be subjected to an Electronic Signature process for approval. For more information on creating and using electronic reports and the electronic signatures functionality see

Electronic Reports


2.7.1

Reviewing Results

The results can be reviewed by clicking the appropriate tab at the bottom of the Work Area of the report template.

You can navigate through the sequence by:

•

Using the

Previous

and

Next

buttons in the

Navigation

group of the

Home

tab.


Chromeleon 7

•

Selecting the injection and/or channel you want to view in the

Navigation Pane.

2.7.2

Printing Results

When printing results, you can:

•

Print the currently selected injection only.

• Print the entire sequence.

Both processes will be described in the following sections.

Tip:

With the autorepeat area function, you can create a single report template that will be suitable for a range of methods, whether a method uses one data channel or several, or analyzes only one compound or several. Chromeleon will automatically report the relevant results. For details, refer to

Define Autorepeat Areas in the Chromeleon Help.

2.7.2.1

Printing Results for a Single Injection

To print the results for the currently selected injection:

1.

Select the

Report Designer

category from the

Chromatography Studio.

2.

In the Navigation Pane, select the injection you want to print out.

3.

On the Studio Menu, select

Print

to open the

Print

dialog box.

4.

From the

Print

dialog box, select:

•

Apply to Current Injection

.

•

The

Printer

you want to use.


Chromeleon 7

•

The

Sheets

to be printed for the current injection.

The results for the current injection are now printed.

2.7.2.2

Print Results for the Entire Sequence

To print results for the entire sequence, follow the steps for

“Printing Results for a Single Injection”, but in the

Print

dialog box:

•

Select

Apply to Current Sequence

.

•

When selecting the

Sheets

to be printed you can click the

Down Arrow

in the

Condition

column to apply print conditions for the selected sheet.

Tip:

To print several injections from a sequence, first select the injection row or rows you want to print, right-click, and then click Print.


Chromeleon 7

2.8

MS in Chromeleon

MS instrument control and data visualization / processing support are provided starting in Chromeleon 7.2. Many of the features that can be used for LC, IC and GC analysis can be used for MS analysis. This section describes the features which are unique to MS.

2.8.1

MS Instrument Control

Direct instrument control is governed by the MS driver and external program provided with the MS hardware. Please refer to the User Manual provided for your specific MS model for instructions on how to use the external program.

2.8.1.1

Creating an MS Instrument Method

Please refer to the User Manual provided with your specific hardware.

2.8.2

Acquiring Data with an MS

Data acquisition with an MS after an MS instrument method is created is identical to data acquisition with non-MS instrument

configurations. Please refer to section 2.5 for more details.

2.8.3

MS Data Visualization

MS data visualization can be done with the spectral plot independent or dependent of the chromatogram plot. The basic functionality is described in this section.


Chromeleon 7

2.8.3.1

MS Plot

The MS Plot can be enabled by clicking the button in the

Panes

group of the


tab (Figure 9).

Figure 9

: Mass Spectra Plot

To display spectra, the user is required to select one of the following:

• A peak from the chromatogram.

•

A component from the navigation pane.

–OR–

•

A data point using the

Time Spectra Tool

in the

Peak Purity

group on the

Layout

tab page of the

Data Processing

Category.

Plot Mode

: MS data can be acquired in either profile (continuous) or centroid (discrete) mode. Profile data can be algorithmically converted to centroid format, but centroid to profile is not possible.

Select a mass spectra plot to display the

Spectral Plot Tools

ribbon.

Then click the

Layout

tab. To view data in centroid mode, click the


Chromeleon 7

Centroid

radio button in the

Plot Mode

group, or

Automatic

in order to view all data as it was originally acquired.

Overlays

: In order to view supplemental spectra, click

or in the

Overlays

group.

The

Overlays

group is equal to the

Plot Mode

on the

Layout

tab page on the

Spectral Plot Tools

ribbon. Click

Reference

to view additional options to select the desired reference spectrum.

Some of the overlay functions, for example the Background overlay, require relevant settings to be defined in the processing method.

Extracted Ion Chromatogram (XIC)

: When chromatographic MS data is acquired, this is displayed as a Total Ion Chromatogram (TIC) which is created by summing all mass peaks for each data point. In order to increase specificity for a component and to reduce background noise contribution, an XIC is created.

An XIC can be created in two manners:

•

Left-click a mass peak in the mass spectrum when the cursor appears.

–OR–

• Right-click a mass peak in the mass spectrum and select

Extract Ion Chromatogram…

from the context menu. The

Extract MS Channel

dialog box opens (Figure 10).


Chromeleon 7

Figure 10:

Extract MS Channel dialog box

Both actions will result in a new chromatographic channel being created from the selected mass-to-charge ratio or range.

2.8.4

MS Data Processing (Processing Method)

The basic principles behind MS data processing are similar to data processing of non-MS data. The differences for MS will be

covered in this section. Please refer to section 2.6 for general

information on data processing.

In order to display MS data processing settings, click the


tab of the

Data Processing

category and select the

All Settings

layout in the

Layouts

group (Figure 11).

Page 40

Figure 11:

All Settings Selection in Layouts group

Quick Start Guide

Chromeleon 7

2.8.4.1

MS Peak Detection Algorithms

In addition to COBRA and Chromeleon 6 algorithms, two new algorithms are introduced – Genesis and ICIS.

•

Genesis:

This algorithm is generally used for processing data with noisy baseline such as MS full scan data. In some instances, the algorithm may also be used with non-MS data as well. These include, but are not limited to UV, CD, and ED data.

•

ICIS:

This algorithm is recommended when dealing with data with very low noise or abstracted data such as that acquired in SIM or SRM mode or extracted post-acquisition as XICs.

2.8.4.2

MS Detection Tab

On this tab page in the processing method, you can define default MS detection settings or detection settings on a component-by-component basis. In the Extracted Ion

Chromatogram dropdown, all components denoted with are using detection settings specific to that component. All other components use default MS detection parameters.

When selecting the ICIS and Genesis algorithms for the first time, all parameters come populated with recommended default values.

2.8.4.3

Component Table – Advanced Tab

In order to process and quantitate components using XICs from

MS data, you are required to define a special channel for the desired components.

In the

Channel

column of the component table, all components being quantitated using an XIC must be set to MS Quantitation

via the dropdown (Figure 12 and Figure 13).


Chromeleon 7

Figure 12:

MS Quantitation selection in Component Table

Figure 13

: MS Quantitation channel

2.8.4.4

Component Table Properties – Extracted Ion

Chromatograms

1.

Select a component by highlighting en entire row in the component table, click

Show Properties

to display the

Properties

dialog box, then select

Extracted Ion

Chromatograms

in the properties navigation pane

(Figure 14).

2.

Click to create a new Quantitation XIC.

If there are currently no XICs defined, the

MS Quantitation


Chromeleon 7

Peak

tab page is added. Otherwise, an

MS Confirming Peak

tab page is created.

In order to assist you define additional detection parameters, Chromeleon displays the mass spectrum at the specified retention time. Note that no mass spectrum might exist at the exact retention time. In this case, the MS

Quantitation Peak tab will show the nearest spectrum to the defined retention time from the XIC.

Figure 14

: Extracted Ion Chromatograms property page

3.

In the

Type

field, select the appropriate selection


Chromeleon 7 a.

TIC

–Typically used for processing of acquisition of nonmultiplexed SIM or SRM experiments. b.

Mass Range

– Typically used for processing of Full Scan experiments.

Note:

If selecting TIC, skip steps 4 and 5.

4.

(Optional) If SRM data was acquired, type in the mass in the

Precursor Mass

field. If a large number of acquisition filters exists, this will assist you in limiting the selectable filters to

only those of interest (Figure 15).

Figure 15

: Filtration of scan list by entering SRM precursor mass

5.

Type in a SIM / SRM product ion mass or mass range in the

XIC Mass(es) or Range(s)

field. Similar to the

Precursor Mass

field, this field will assist in limiting the selectable filters to


Chromeleon 7 only those of interest.

In addition, this field will also define which mass(es) are used to create the XIC.

6.

Select the appropriate filter from the Filter field dropdown.

Based on the combination of the

Filter

and

XIC Mass(es) or

Range(s)

, an XIC for evaluation will be created in this UI

(Figure 16).

Figure 16:

XIC displayed based on parameters

(Optional steps to set up Confirming XIC for component)

7.

Click to create a new Confirming XIC.

8.

Repeat steps 3 through 6 to setup the XIC for a confirming peak.

9.

Click on

Enable

underneath the Ratio header.

10.

Set the expected

Target Ratio %

(peak area) relative to the

Quantitation peak.

11.

Select Relative or Absolute tolerance for the Target Ratio

Window Type.

12.

Set the tolerance

Window %

.

If the target ratio is set to 50 %, the Window to 10%, the peak area ratios falling within the following windows will result in a confirmed component:

Absolute:

40 – 60%


Chromeleon 7

Relative:

45 – 55%

13.

Set the absolute tolerance in minutes for the confirming peak in the

Ion Coelution

field.

After completion of these steps, combined with the steps outlined in section 2.6, it will be possible to review and report out on the resultant data. See the

Extracted Ion Chromatograms

section of the Chromeleon Help for more information.


Chromeleon 7

3 Appendix: Chromeleon Overview

3.1

Introduction

Chromeleon is a Chromatography Data System that allows you to control chromatography instruments and evaluate, process, report, and backup data.

Chromeleon has several software components:

Instrument Controller Service

: The Instrument Controller Service handles communication with and data acquisition from the connected instruments.

Client

: The Chromeleon Client provides the user interface for all tasks related to instrument operation, data analysis, and data management.

Services Manager:

Shows the status of the Chromeleon

Instrument Controller Service and provides a user interface to control this service. The status of all other Chromeleon services is also displayed.

Instrument Configuration Manager: Allows configuration of instruments connected to the Instrument Controller PC.

Administration Console: Central access point for administrative tasks, like:

•

License Manager

: Provides tools for managing licenses for

Chromeleon stations and users.

•

Scheduler

: Provides tools for scheduling data transfer.


Chromeleon 7

•

User Database

: Provides tools for managing system policies, users and roles, access privileges, and operational privileges.

•

Global Policies

: Provides settings for global Chromeleon policies.

•

Discovery

: Central repository containing information about the available instruments and Data Vaults.

•

Local Machine

: Provides tools for managing local

Chromeleon 7 Data Vaults (Create, Mount and Dismount) and Chromeleon 6 Datasources (Mount and Dismount), and enables you to change the Chromeleon Domain Controller.


Chromeleon 7

3.2

Instrument Controller Service

The Chromeleon Instrument Controller Service handles all realtime instrument processes (communications, control, and data acquisition). It runs in the background as a Windows service on

PCs to which instruments are physically connected. Its status is represented by an icon in the Notification Area of the

Windows Taskbar (Figure 17).

Figure 17:

Notification Area of Windows Taskbar for Windows XP and Windows 7

You can add, configure, or delete instruments and modules, connect to instruments, and track changes made to instruments from the Instrument Configuration Manager.


Chromeleon 7

3.3

Chromeleon Client

The Chromeleon Client provides the user interface for all tasks related to instrument operation, data analysis, and data management.

The Chromeleon Client contains two windows:

Chromeleon

Console

:

Chromatography

Studio

:

The main Chromeleon window in which you can control instruments, navigate through all data, and use or manage eWorkflows.

A separate window that is launched from the Console, in which you can work with data and objects related to a selected sequence. Here you can view and optimize all aspects of the data, modify instrument methods and processing methods, define and generate reports, and manage spectral libraries.


Chromeleon 7

3.3.1

Chromeleon Console

When you start Chromeleon 7, the Console is the first window to

appear. The Console window has three major parts (Figure 18).

Navigation Pane

Work Area

Sequence

Control Bar

Category Bars

Figure 18:

Chromeleon Console

Status Bar


Chromeleon 7

1.

Category Bars

: Use the category bars to switch between the three categories:

Instruments

,

Data

and

eWorkflows

(Figure

19).

Figure 19:

Category Bars

2.

Navigation Pane

: Here you can navigate the objects related to the selected category.

3.

Work Area

: This area shows the content of objects selected in the Navigation Pane. Here you can work on Chromeleon objects.

There are three additional parts for user interaction:

The Navigation Pane has a top-level filter that lets you control which instruments, data vaults, or eWorkflows are displayed: a.

Local

: Items available on the local Chromeleon station. b.

c.

All

: Items available on the Chromeleon Domain.

Favorites

: Filter for displaying commonly used items. d.

Custom

: Filter based on text input.

The

Sequence Control Bar

above the injection list indicates the sequence status (

New

,

Running

,

Complete

,

Interrupted

,

Incomplete

) and can be used to start and stop a sequence. It is only available when a Sequence is selected in the Navigation

Pane.


Chromeleon 7

Note:

When a sequence is configured to be electronically signed, additional statuses are available.

The

Status Bar

shows general information such as the currently logged on user.

3.3.1.1

Instruments

The

Instruments

category provides access to all instruments on the network (this might be limited by user privileges).

When the Instruments category is selected, the configured instruments are displayed in the Navigation Pane . The Work

Area gives details about the instrument selected in the

Navigation Pane.

From the Work Area you can review instrument status and make changes to instrument parameters. Across the top of the work area a series of ePanel tabs are displayed. ePanels can be used to monitor instrument operation and to directly control the instrument.

Note:

When you select an instrument from the Navigation

Pane that is currently offline, a message is displayed in the

Work Area.

3.3.1.2

Data

The

Data

category provides access to all data stored in each connected Data Vault ( container for all data).

Note:

Chromeleon 6 Datasources are visible in

Chromeleon 7. It is not possible to make changes in these

Datasources, but data can be copied from them to

Chromeleon 7 Data Vaults.


Chromeleon 7

Data can be accessed and organized from the Navigation Pane.

The Work Area shows the content of the selected object and allows you to edit the object.

A Data Vault can contain the following objects:

•

Folder

: A container for objects. Use folders to organize data in a Data Vault.

•

•

•

Sequence

: A collection of injections that belong together.

A typical sequence contains an instrument and processing method, a report template, and a view settings file.


: A collection of timed commands for an instrument to perform during a chromatographic analysis.


: A collection of parameters (including parameters for peak detection and calibration) which are used when evaluating chromatograms.

•

Report Template

: A file which defines how data is displayed, printed, or exported. A report template can also contain calculations. When applied to a sequence (to view, print, or export the results) the outcome is referred to as a report.

•

Electronic Report

: An electronic version of the results of a sequence.

•

View Settings

: The definition of how data is presented on the screen.

•

UV Spectral Library

: A collection of spectra used for peak identification.

•

Query

: A Data Vault search.


Chromeleon 7

•

Instrument Audit Trails

: A log of instrument events such as system settings, executed commands, and error messages.

A sequence is a collection of injections that are analyzed and processed together in series. Each injection is represented by a row in the sequence (replicate injections are represented with multiple rows). Injections can have one of the following

Injection

Types

:

•

•

Unknown

: A sample for which the quantity, presence, or absence of components is to be determined during the analysis.

Calibration Standard

: A sample with known concentration(s) of component(s). Calibration Standards are used in the construction of calibration curves.

•

•

Check Standard

: A sample with known component concentration(s). Check Standards are used to verify a calibration.

Blank

: An injection used to characterize the background signal (baseline), and/or to ensure that unwanted components are not detected. Blank “injections” can be made without an actual injection taking place.

•

Matrix

: The sample matrix. The peak areas in the Matrix can be subtracted from the corresponding peak areas in all the other injections in the sequence.

•

Unspiked

: An Unknown sample to be analyzed with the

Standard Addition method.


Chromeleon 7

•

Spiked

: An Unknown sample to be analyzed with the

Standard Addition method, with known amounts of components added.

3.3.1.3

eWorkflows

eWorkflows are electronic procedures for automating laboratory processes related to a chromatographic analysis.

An eWorkflow contains a set of templates (such as instrument methods, processing methods, report templates, view settings, and/or spectral libraries) and a set of rules. The rules define, for example, the sequence layout (such as the maximum number of

Unknown injections allowed between Calibration Standards), and the list of instruments in the network that are suitable for the analysis. When you start an eWorkflow, it will create a sequence with predefined associated files and a well-defined structure.

The list of available eWorkflows is visible in the Navigation Pane.

An overview of the instruments (including their status) that can be used with the selected eWorkflow is displayed in the Work

Area. From here you can launch an eWorkflow and will be guided in creating an appropriate sequence.


Chromeleon 7

3.3.2

Chromatography Studio

In the Chromatography Studio you can edit all objects related to the selected sequence:

•

Injection List

•

Instrument Methods

• Processing Methods

• View Settings

•

Report Templates

•

Electronic Reports

•

Spectral Libraries

When you open a sequence from the Console, the

Chromatography Studio (Figure 20) is opened.

Note:

When you open an object outside the context of a sequence, only the corresponding category of the Studio is available.


Chromeleon 7

Studio Menu

Navigation Pane

Ribbon

Work Area

Category

Bars

Figure 20:

Chromatography Studio

The Chromatography Studio is structured similarly to the

Chromeleon Console, and consists of the following major parts:

•

Category Bars

(Figure 21): These switch the display between

the six categories:

Injection List

,


,

Data

Processing

,

Report Designer

,


and

UV

Spectral Library

.


Chromeleon 7

Figure 21:

Category Bars

•

Navigation Pane

: An area in which you can navigate through the objects available in the selected category.

•

Work Area

: The content changes according to the selected category.

There are two additional parts for user interaction:

•

Ribbon

: The menu tool for main commands in the Studio.

Commands are organized in groups, which are collected together under tabs. To reduce clutter, some tabs are only shown when needed.

•

Studio Menu

: To access the Studio Menu, click the

Chromeleon icon in the top left corner of the

Chromatography Studio window. From this menu you can access general tasks, such as printing, exporting, and saving.

3.3.2.1

Injection List

When the

Injection List

category is selected, you can edit, print, and export the injection list.

The Navigation Pane gives an overview of all files used in the sequence. The Work Area displays further details about each


Chromeleon 7 injection, such as the type of sample (for example Calibration

Standard) and the injection status.

Note:

You can also edit the injection list directly in the

Console. If a sequence is open in both the Console and the

Studio, changes made in either window are automatically made in the other, so that copies of the sequence stay synchronized.

3.3.2.2


When the


category is selected, you can edit instrument methods.

The Navigation Pane lists the instrument methods in the sequence and provides an overview of the instrument’s modules.

The Work Area lets you edit the instrument method.

3.3.2.3

Data Processing

When the

Data Processing

category is selected, you can view chromatography and mass spectrometry results, edit the processing method, and manually integrate chromatograms.

The Navigation Pane gives an overview of all injections, channels, processing methods, and view settings used in the sequence.

Chromatographic results are shown in the Work Area.

You can customize the panes displayed (such as Chromatogram,

Interactive Results, Calibration Plot, and Processing Method) on the Ribbon by selecting or hiding each pane in the

Panes

group of the


tab (Figure 21).


Chromeleon 7

Figure 22:

Pane Selection in Ribbon

Tip:

The Presets group (left of the Panes group in the Data

Processing Home tab) offers typical combinations of panes.

3.3.2.4

Report Designer

In the Report Designer category, you can view reports and edit report templates.

The Navigation Pane shows a list of all channels, injections, and report templates available in the sequence. In the Work Area you can view and modify the report.

3.3.2.5


In the Electronic Report category, you can create and manage electronic reports. This is an electronically captured snapshot of the results.

The Navigation Pane shows a list of all injections and sheets available in the electronic report and the electronic report itself.

The Work Area shows the electronic report, or, when no electronic report is available, the option to create one.


Chromeleon 7

3.3.2.6

UV Spectral Library

The UV Spectral Library view of the Chromatography Studio provides tools for managing and comparing spectra extracted from 3D Field data.

3.3.2.7

Menus

Chromeleon 7 Studio commands are accessible via the Ribbon or

Studio Menu. To access the Studio Menu, click the Chromeleon icon .


Index

Acquiring Data

Administration Console

Blank

Calibration

Calibration Curves

Calibration Levels

Calibration Standard

Category Bars

14, 37

47

55

30

32

31

31, 55

52, 58

Check Standard

Chromatogram

Client

Cobra

55

19

47, 50

21, 23

Component Table 27, 29, 42, 43

Confirming Peak 46

6 Connect

Console

Data Processing

Data Vault

Data Vault Manager

Detection Parameters

Discovery Service

Electronic Report

4, 50, 51

17, 60

53, 54

48

21

48

54, 61

50, 56 eWorkflows

Extracted Ion Chromatogram 39

Genesis

ICIS

41

41

26

59

55

5, 53

Identifying Peaks

Injection List

Injection Types

Instrument

Instrument Configuration

Manager

Instrument Controller

Service

Instrument Method

Integration

Interactive Results

Ion Coelution

License Manager

Mass Range

Mass Spectrometry

47

47, 49

9, 54, 60

20

18

46

47

44

37

Quick Start Guide

Chromeleon 7

Query

Queue

Rack View

Report

Report Designer

Retention Time

Reviewing Data

Ribbon

Scheduler

Sequence

Services Manager

SmartPeaks

Spectral Library

Spiked

Start

Studio

Studio Menu

Target Ratio

TIC

Unknown Injection

Unresolved Peaks

Unspiked

User Manager

Work Area

XIC

Matrix

Module

Monitor

MS Channel

MS Data Visualization

MS Instrument Control

MS Instrument Method

MS Plot

Navigation Pane

Overlays

Peak Detection

Plot Mode

13, 52, 59

39

20, 41

38

Precursor Mass

Print

44

35, 36

Processing Method 10, 29, 40, 54

Quantitation

55

6

7, 16

39, 42

37

37

37

38

30, 42

54

14

11

10, 34, 35, 54, 61

58

30

17, 34

59

47

9, 10, 36, 54

47

25

54, 62

56

4

17, 50, 57

59, 62

46

44

55

25

55

48

52, 59

39, 45, 46

Page 63

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Quick Start Guide - Chromeleon 7.2

Table of Contents

1 Introduction

1.1

About this Document

1.2

Other Documentation

2 Using Chromeleon

2.1

Overview

2.2

Starting Chromeleon

®

2.3

Getting your Instrument Started

2.4

Creating a Sequence

2.5

Acquiring Data

2.6

Processing Data

2.7

Reviewing and Reporting Results

2.8

MS in Chromeleon

3 Appendix: Chromeleon Overview

3.1

Introduction

3.2

Instrument Controller Service

3.3

Chromeleon Client

Index

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