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U s s e r M a n u a l l E L I I S A P L A T E R E A D E R
ELISA PLATE READER
DIA2000
Version – 3.400A
DIAsource ImmunoAssays S.A.
Rue du Bosquet 2 B-1348 Louvain-La-Neuve
BELGIUM
Phone : +32 10 84 99 11
Fax : +32 10 84 99 90 www.diasource.
be
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TABLE OF CONTENTS
1. GENERAL INFORMATION
...................................................................................................... 5
1.1. Warranty Information
..................................................................................................................... 5
1.2. Technical Service
.............................................................................................................................. 5
1.3. Contacts
............................................................................................................................................ 5
2. GENERAL SAFETY WARNINGS
.............................................................................................. 6
2.1. Danger – warning symbols
............................................................................................................... 6
2.2. Use of the Instrument
....................................................................................................................... 7
3. INTRODUCTION
........................................................................................................................ 8
3.1.
Description ....................................................................................................................................... 8
3.2.
Special Features ............................................................................................................................... 8
3.3. Technical Specification
..................................................................................................................... 9
4. PACKAGING, TRANSPORT AND STORAGE
........................................................................ 11
4.1.
General Warnings .......................................................................................................................... 11
4.2.
Packaging ....................................................................................................................................... 11
4.3. Instrument Transportation
............................................................................................................ 11
4.4.
Storage of Instrument .................................................................................................................... 11
5. INSTRUMENT DESCRIPTION
............................................................................................... 12
5.1.
Instrument Working Principle ...................................................................................................... 12
5.2.
Perspective View ............................................................................................................................. 14
5.3. Keypad
............................................................................................................................................ 15
6. INSTALLATION PROCEDURE AND VERIFICATION CRITERIA
...................................... 17
6.1. Unpacking Instructions
.................................................................................................................. 17
6.2. Placing the Instrument
................................................................................................................... 17
6.3. Power Supply Requirements
.......................................................................................................... 17
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6.4. Protective Grounding
..................................................................................................................... 17
6.5. Printer
............................................................................................................................................. 17
6.6. Start up Instructions
....................................................................................................................... 19
6.8.
Micro Titer Plate Carriage ............................................................................................................ 21
6.9.
Plate Loading & Pipetting Procedure ........................................................................................... 21
6.10. Readings Check
............................................................................................................................ 22
7. PRECAUTIONS
........................................................................................................................ 22
8. PROGRAMMING MODES
....................................................................................................... 23
8.1. Absorbance Mode
........................................................................................................................... 23
8.2. Single Standard
.............................................................................................................................. 26
8.3.
Cut Off Mode ................................................................................................................................. 27
8.4. Reverse Cut Off Mode
.................................................................................................................... 43
8.5. Multi Standard Mode:
.................................................................................................................... 44
8.6. Percentage Absorbance
.................................................................................................................. 55
8.7. Uptake
............................................................................................................................................. 55
8.8.
Kinetic ............................................................................................................................................. 56
9. RECALLING AND RUNNING OF STORED TEST/PROGRAMS
.......................................... 57
9.1. Absorbance Mode:
.......................................................................................................................... 58
9.2. Cut off Mode
................................................................................................................................... 62
9.3. Multistandard
................................................................................................................................. 69
10. RERUNNING ACCEPTED TESTS / PROGRAMS
................................................................ 81
11.SAMPLE AND SAMPLE DUPLICATE
.................................................................................. 85
12.PATIENT IDENTITY / SAMPLE IDENTITY ENTRY
........................................................... 87
13.SAVE PLATE AND SAVE RESULTS
..................................................................................... 90
14.VIEW STORED DATA
............................................................................................................. 92
15. UTILITIES
.............................................................................................................................. 94
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15.1. Date & Time Setting
..................................................................................................................... 95
15.2. Program Filter
.............................................................................................................................. 95
15.3. Printer On / Off setting:
............................................................................................................... 96
15.4. Enter Clinic Name:
....................................................................................................................... 96
15.5. Shaker (Plate Shaking Mode)
....................................................................................................... 97
15.6. Communication Settings:
............................................................................................................. 98
15.8. Install USB Driver:
....................................................................................................................... 99
15.9. Auto Print: ON
............................................................................................................................. 99
15.10. PASSWORD:
............................................................................................................................ 100
16. SERIAL COMMUNICATION
............................................................................................... 103
17.1. Clear Memory
............................................................................................................................. 109
17.2. Lamp Amplitude
......................................................................................................................... 110
17.3. Printer Self test
........................................................................................................................... 112
19. DECONTAMINATION
......................................................................................................... 115
19.1. Decontamination Procedure ........................................................................................................115
19.2 Purpose of Decontamination ........................................................................................................115
19.3. General Considerations ...............................................................................................................115
19.4. Procedure .....................................................................................................................................115
20.
SAFETY CLEARANCE CERTIFICATE .............................................................................. 116
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1. GENERAL INFORMATION
1.1. Warranty Information
Each Instrument is completely tested and guaranteed for twelve months from delivery
The warranty applies to all the mechanical and electrical parts. It is valid only for proper installation, use and maintenance in compliance with the instructions given in this manual.
DIASOURCE IMMUNOASSAYS S.A.
will, at its discretion repair or replace parts, which may be found defective in the warranty period. The warranty does not include any responsibility for direct or indirect personal and material damages, caused by improper use or maintenance of the instrument.
Parts that are inherently subject to deterioration are excluded from the warranty. In case of defects due to misuse of the instrument, any travel and man-hour expenses will be charged extra.
1.2. Technical Service
DIASOURCE IMMUNOASSAYS S.A.
is always accessible to the customers for any kind of information about installation, use, maintenance and others. When asking for service, please refer to this manual and report the data reported on the identification label (serial number).
Only qualified technicians are entitled to repair instruments. The user should carry out ordinary maintenance.
1.3. Contacts
DIASOURCE IMMUNOASSAYS S.A.
Rue du Bosquet 2
BE-1348 Louvain-La-Neuve
BELGIUM
IVD Instrumentation Support Service
Hotline phones availabilities : Monday to Friday; 08 :30 to 17:00 (Belgium time)
Please contact first Hotline 1. In case of no response please contact Hotline 2.
Hotline 1 phone number : 0032 (0)10 849932
Hotline 2 phone number : 0032 (0)10 849976
Fax : 0032 (0)10 849990
Email :
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2. GENERAL SAFETY WARNINGS
2.1. Danger – warning symbols
This is a symbol of generic danger. It means that, serious damage can occur to the operator if described precautions are not observed.
This is a symbol of HIGH ELECTRIC VOLTAGE. It is dangerous to touch any part having this label. Only qualified operators can access these components, after unplugging the instrument from the supply.
This symbol indicates that the instrument makes use of chemical reagents and other dangerous (Corrosive, irritant, or harmful) CHEMICAL SUBSTANCES, which can cause damage to people or materials. When this label is found, pay
attention to the manufacturer’s recommendations.
This symbol indicates that the instrument involves the handling of samples, which
can be infected (urine or human serum). In this condition, infection or contamination might occur. Pay attention to the general safety warnings when in presence of such biological substances. Use protective clothes, gloves and glasses.
This symbol in the user manual indicates that damages to the instrument or erroneous results could occur if the given warnings are not respected.
This symbol indicates a portion, which is particularly important, and should be studied carefully.
This symbol indicates a Protective Earth or Ground terminal.
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2.2. Use of the Instrument
1. The instrument has to be used for the designed purposes under specified conditions,
following proper procedures and safety rules by qualified personnel.
2. This manual contains instructions for operation performed by qualified personnel.
3. A qualified user has to make sure that environmental condition is suitable, the installation
is correct, the use and maintenance are proper according to the general safety rules as well
as to the particular precautions described in the manual. (However, he is not entitled to
repair the instrument).
4. A qualified technician is entitled to maintain and repair the instrument using the original
spare parts according to the given instructions.
5. Maintain room temperature and humidity as specified in the manual.
6. If the instrument is not used as described in the manual, the protection provided by the
instrument may be impaired.
7. Alterations to the instrument are prohibited. The user is liable for any improper
Modification to the instrument, and for the deriving consequences.
8. Contact the MANUFACTURER service or authorized service center in case the instrument
need extraordinary maintenance. Specialized technicians who will be able to repair the
instrument using original spare parts will carry out the maintenance.
9. This IVD equipment complies with the emission and immunity requirements as per
IEC61326 series.
10. Warning : This equipment has been designed and tested to CISPER11 Class A. In a domestic environment it may cause radio interference, in which case, you may need to take measures to mitigate the interference."
11. An advisory that the electromagnetic environment should be evaluated prior to operation of
the device.
12. Warning : Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g. unshielded international RF sources), as these may interfere with the proper operation.
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3. INTRODUCTION
3.1. Description
The ELISA PLATE READER is a user friendly micro plate Analyser. It is compact &
lightweight. It is designed to measure and interpret enzyme immunoassay results, both
monochromatically and bichromatically. It is intended for in vitro diagnostic use.
3.2. Special Features
The ELISA READER can accommodate a flat bottom as well as a round configuration.
The carriage is designed in a way that the plate automatically moves smoothly and
positions iself accurately in the optical measurement path. Readings are taken
continuously. The average value is calculated and results are presented according to the
option selected.
The ELISA READER operates on a WIDE voltage (90-270 volt). This eliminates the need for an external voltage stabilizer.
The ELISA READER has a special provision, which allows it to be used even when a
printer is not available. Readings can be conveniently noted down manually.
The other special features of ELISA READER are as follows:
Option of Lamp saving mode.
Selection of both primary and secondary filters.
Latest technology with battery back-up for 250 tests with QC, more than 2500 result.
Robust built in 52-column thermal printer with 384 stationary heads.
Unique circuitry for long lamp life.
Alphanumeric Patients ID entry.
Editing of saved tests.
Human machine user interface: Touch panel, Keypad
Multi-standard curve up to 12 standard calibrations with one blank optional.
Access to test by touch of key.
Capability to connect to 80-column printer for direct report printout.
Blank is optional.
Setting of the Date and Time.
Capable of storing, deleting and recalling tests.
Multiple calibrator modes.
Selection of duplicates for both calibrators and samples.
Extensive software for cut off mode.
Selection of Positive, Equivocal, Negative cut-off.
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Several pre-programmed calculation modes will help to facilitate data processing of enzyme immunoassays. These are menu driven modes for simple and error free operations.
Human Machine Interface
ABSORBANCE MODE
CUT-OFF MODE
MULTISTANDARD MODE
SINGLE CALIBRATOR MODE
% ABSORBANCE MODE
UPTAKE
KINETIC
3.3. Technical Specification
TOUCH PANEL / KEYPAD
0.000 to 3.000 Absorbance Units (A) Linear measurement range
Photometric Accuracy
± 2% or 0.007 whichever is higher, from 0 to 1.5 A
± 3% from 1.5 A to 3.0 A
Drift
<0.005 A/hr
Photometric Linearity
2.5 A
Optical measurement
8 Channel
Filters
Type of filter
Wave Length
Half Bandwidth
Selection
Narrow band Interference
405nm, 450nm, 492nm, 630nm, 560nm
10nm ± 2nm
Automatic by Stepper Motor
Light Source
Display
Curve Plotting
Tungsten halogen lamp, 20 Watts
6” Graphics LCD, Negative Blue, STN
Graphical Representation on Printer
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Plate Carrier Movement
Printer
Memory
Analysis Mode
Connectivity / RS232 Serial Port / USB
Power
Wattage
Voltage
Operation Position
Operating Conditions
Temperature
Relative Humidity
Storage Conditions
Temperature
Relative Humidity
Enclosure
Size (cm)
Weight (Approx.)
Precisely through the stepper motor
Built in Thermal Printer 52 columns
Battery backup supporting 2500 open channels
64 KB Non Volatile RAM
Absorbance
Single Standard
Cut-off
Multi-Standard
% Absorbance
Uptake
Kinetic
9600 baud, 8 data, 1 stop, no parity bits / USB
75 Watts
115-230 Volt ± 10%, 50/60 hz.
On horizontal flat, rigid and vibration free surface
From + 18°C to 35°C
Up to 80%
From -10°C to 40°C
Up to 80%
ABS Fire Retardant
36 x 36 x 22 (lxbxh)
10 Kgs
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4. PACKAGING, TRANSPORT AND STORAGE
4.1. General Warnings
Instrument has to be decontaminated before packing for transportation.
4.2. Packaging
Packaging is needed whenever the instrument is to be transported or shipped by courier or other purposes.
To pack the instrument the following instructions has to be followed:
1. Decontaminate the instrument as explained on decontamination chapter of this manual.
2. Put the instrument into the original packaging box; Instrument has to be properly
protected by plastic protective material. Put copy of Safety clearance certificate (copy of
Safety clearance certificate is attached at the end of this manual)
3. Mark the packaging with address, instrument identification and warning labels
4.3. Instrument Transportation
The transportation of the instrument in unpacked condition must be limited within the room where it is used, to avoid damage.
4.4. Storage of Instrument
Before storing the instrument for a long period, pack it carefully as described above and store indoors.
Relative humidity can be up to 80%, and temperature between -10ºC and 40ºC.
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5. INSTRUMENT DESCRIPTION
5.1. Instrument Working Principle
1. Switch on and program the instrument.
2. Place the plate firmly onto the carriage.
3. Start the test, the plate will be conveyed inside and strip is placed precisely below the
optical path. The measurement is carried out step by step.
At the end of the cycle the results are printed at the same time they are available at the RS232 serial port output.
On starting the test, the plate will be conveyed into the instrument.
Plate is moved in step by step by a well-controlled mechanism and each well is positioned precisely below the optical path. Optical density of each well is measured as explained below.
Based on selected programming mode, sophisticated algorithms are used for analyzing the measured value and presenting the same on the printer, display & RS232 serial port for computer
Interface.
The following diagram represents the main functional elements of the instrument:
White light produced by the lamps is focused into a beam by the lens and passes through the sample. Part of the light is absorbed by the sample and the remaining light is transmitted. It is filtered by interference filters and focused onto the photodiodes. The photodiode converts the received light into an electrical signal which is transformed into a digital form, from which the microprocessor calculates the absorbance, taking in account of the blank and bichromatic selection.
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Lamp
Lens
Memory
Wells
Lens
Filter
Lens
Analogue to Digital
Converter
Photo sensor
Amplifier
Micro Processor
Key Pad
Touch Panel
Printer
RS232
Display
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5.2. Perspective View
Front View:
Printer
Touch Screen
Rear View:
Contrast knob
Cooling Fan
USB output
1
2
3
7
4
5
Serial RS232 O/P
Ext. Printer connection
1) Contrast Knob-use to adjust display contrast
2) Serial RS232 output 3) Cooling Fan 4) USB output
SMPS
6
Keypad
Plate Carrier/ Holder
On / Off switch
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5.3. Keypad
E L I I S A P L A T E R E A D E R
1) ‘YES’ key is used to select any ‘YES’ option on screen directly.
2) ‘NO’ key is used to select any ‘NO’ option on screen directly.
3) ‘PRINT’ key is used to get a printout of current screen displayed.
4) ‘TEST’ key is used for directly accessing the RUN screen of the selected test.
5) Navigation keys are used to select any option available on current screen.
6) ‘ENTER’ key is used to run any option selected by navigation keys.
7) ‘ESC’ key is used for escaping from any screen.
8) ‘SHAKE’ key is used for directly accessing the shaking mode (ie. SHAKER)
(refer 15.5 for more details on SHAKER)
(Note: Functions based on HOME & FEED keys operates only when it is in List Test screen or the first screen after initializing)
ELISA READER 18/07/12
Clinic name / Serial Number
Select Test
MENU
1. HBSAg 6. PER_ABS RUN / SEL
2. HCVAb 7. CRP PRNT
3. UPT 8. KIN_405 NEXT
4. TSH 9. SIN_630 PREV
5. CPC 10. CP_560 DEL
PLATE
DATA
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User can select “List Test” screen as shown below-
1 Test Operations
MAIN MENU
2 Technical Diagnosis
3 Utilities
4 View Plate
5 Serial Communication
ESC
TEST OPERATIONS
1 Select Test By Name ESC
2 Select Test By Mode
3 Select Test By Number
4 List Tests
5 Add New Test
Select Test ESC
1 HBSAg S
2 HCVAb M RUN / SEL
3. UPT U
4. TSH M PRNT
5. CPC C
6. PER_ABS P NEXT
7. CRP C
8. KIN_405 K PREV
9. SIN_630 S
10 CP_560 C DEL
PLATE
DATA
Select “TEST” button present on the keypad. It will display following screen for selecting the particular test. In this way user can directly run the selected test by just entering the valid test no.
Select Test ESC
1 HBSAg S
2 HCVAb M RUN / SEL
3. UPT U
4. TSH M PRNT
5. CPC C
6. PER_ABS P NEXT
7. ABS A
8. KIN_405 K PREV
9. SIN_630 S
10 CP_560 C DEL
Numeric Value: 7
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
Name: ABS NORM – ABSORBANCE
Pri : 450 Sec : 560 BL N
ABS ABS ABS ABS ABS ABS
1 2 3 4 5 6
ABS ABS ABS ABS ABS ABS
7 8 9 10 11 12
Is Plate Loaded ? YES / NO
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6. INSTALLATION PROCEDURE AND VERIFICATION
CRITERIA
6.1. Unpacking Instructions
Check accessories as per packing list.
Kindly store all packaging materials so as to use it to repack and ship for maintenance
or servicing.
6.2. Placing the Instrument
The instrument has to be placed on a level bench.
Room temperature has to be between 10 to 35ºC with a relative humidity below 85%.
Protect the instrument from direct sunshine.
6.3. Power Supply Requirements
Plug the instrument into a power source by the locally available approved plug in cable.
Power cord should be CE, CSA, and UL marked.
115 - 230 Volt ± 10%, 50-60 Hz
6.4. Protective Grounding
Please make sure that electrical power source is properly grounded.
6.5. Printer
ELISA READER Analyser is equipped with a built in 52 columns easy load thermal
printer. Procedure to load the paper is as follows:
Opening the Paper Cover:
Slide the LEVER towards back of the printer to open lock of the Paper Cover.
Lightly push up on the front of the paper cover (marked) with your thumb and rotate it towards the back of the printer.
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Closing the Paper Cover + replacement of paper roller:
Close the paper cover and push down till you get locking sound.
Open the paper cover.
Remove the paper roller from the slot and place the paper roll over it as shown.
Place back the paper roller with paper into the slot provided and close the paper cover as described before.
Keep pressing the paper feed switch until the paper feeds straight and smoothly.
HINT:
When the paper is set correctly and when the closing of the paper cover is proper the FEED LED will not glow.
It keeps on flashing when the data is being printed.
When the paper is almost finished, red lines appear on both sides of the paper.
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6.6. Start up Instructions
Switch on the instrument.
The instrument initializes all the parameters internally and carries out a power on self-test
During Initializing it displays the string: “Please wait system initializing.”
It will display the ADDRESS screen after completing the initialization.
ELISA READER
DIASOURCE IMMUNOASSAYS S.A.
Rue du Bosquet 2 - B-1348 LOUVAIN-LA-NEUVE
Tel : 00 32 10 84 99 11
Fx : 00 32 10 84 99 90
The instrument will print the model name “ELISA READER”, VERSION NUMBER, CLINIC
NAME, CURRENT DATE WITH TIME.
ELISA READER Date
Clinic name / VERSION Number MENU
Select Test
1. HBSAg 6. PER_ABS RUN / SEL
2. HCVAb 7. CRP PRNT
3. UPT 8. KIN_405 NEXT
4. TSH 9. SIN_630 PREV
5. CPC 10. CP_560 DEL
PLATE
DATA
User can select a saved test by first touching the required “Test Name” on the screen and then touch the “SEL” option to carry out further operation on the test.
“RUN” option is used to directly run the selected test.
“DEL”: Deletes the selected test.
If the numbers of tests are more than 10, “NEXT” and “PREV” options can be used for browsing through the list pages.
“PRINT”: For printing the test screen.
“MENU” used to select Main menu screen.
"PLATE DATA” this option helps the user to view the Plate Results easily by selecting this option present on the screen.
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6.7. Touch Panel Check
ELISA READER provides a Touch sensitive LCD panel and a KEYPAD for easy use interface.
The Menus are displayed and the text of the parameter forms the TOUCH ZONE.
Touch screen Layout
Name:
Pri: 405 Sec: 0
No Factor QC
BL 0 N
NC 0 1.000 N
PC 0 1.000 N
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
EDIT
QCVal Mes. Rem RUN
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
Above is the generic representation of a Test Screen. The Highlighted zones are TOUCH
ZONES, which are active. On touching the “Touch Zone” of a parameter, a sub menu/menu is displayed or the requested action is carried out. The rest of the “Touch Zone” is deactivated.
For Example: To activate the selection:
Enter the primary filter value to touch any point in the shaded area “Pri” on the LCD screen.
On proper selection the analyser responds with blinking of the parameter text and also the
TOUCH ZONE and a submenu is displayed.
To enter Test Name: Touching the “Name” touch zone provides an alphanumeric screen.
Enter the Test name by touching the Touch zone of that variable. The selected value blinks and is displayed next to the parameter.
Selection Indicator
Selected on screen item is shown in a shaded background. When the screen first displays, the default selection is shown. Pressing a selection either highlights that item or activates it.
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6.8. Micro Titer Plate Carriage
The instrument is provided with a micro titer plate carriage to move the micro titer plate inside.
This carriage is driven by stepper motor with timing belt. It places each well of plate exactly below the optical path of each channel. The plate carrier is moved by a well controlled stepper motor drive.
6.9. Plate Loading & Pipetting Procedure
Control and sample pipetting procedure in case of cut off:
Maximum number of Blanks = 5
Maximum number of Controls:
1. Negative Control = 05
2. Positive Control = 05
3. Cut of control = 05
4. Low Positive Control = 05
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Pipetting sequence should be as shown below.
6.10. Readings Check
Checking of readings should be done through controls reading within range specified in data sheet of controls (care should be taken while preparing and pipetting controls and reagents, reagent and control expiry dates need to be checked).
7. PRECAUTIONS
Do not use any sharp objects on the Touch Screen. Always use the STYLUS provided to operate the touch panel.
Always check for proper grounding before installation. Never operate the instrument when ground wire is removed.
3. Do not attempt to open the instrument and make repairs without proper technical
training. Do not allow unauthorized persons to operate or repair the instrument.
4. Use a clean plate and follow the instructions for blanking and standardizing. Do not
read any wells containing bubbles or dust particles.
5. The volume of sample, calibrators and blanks should be identical for correct readings.
The absorbance is proportionate to the path length. Pipetting should also be proper.
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6. Monitoring of the printed values or displayed values during operation may help detect
an error. Check the linearity and calibration of the instrument regularly against some
standard reference.
7. Check the micro wells before use. They should be scratch-free. The micro well track in
the ELISA READER has been designed in such a way that the micro wells are totally
protected from scratches.
Recheck the reading of high OD (above 2A).
9. Place Plate carefully on the tray.
10. Ensure that the main power switch is in OFF position before connecting.
11. Plug the instrument to the AC mains. Confirm proper grounding for trouble
free operation.
12. Connect the printer only when the instrument is OFF.
8. PROGRAMMING MODES
We would like to highlight that the assay protocols reported in this section correspond to illustrations. When programming the instrument, the end-user must always refer to the instruction for use specific to each assay.
8.1. Absorbance Mode
In this mode the instrument gives only absorbance of all wells of plates of the respective plate.
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Programming a New Test:
A. Select “Test Operation” B. Select “Add New Test”
MAIN MENU
1 Test Operations
2 Technical Diagnosis
3 Utilities
4 View Plate
5 Serial Communication
ESC
TEST OPERATIONS
1 Select Test By Name ESC
2 Select Test by Mode
3 Select Test by Number
4 List Tests
5 Add New Test
C. Select mode of operation “Absorbance” in “Add new test”
1> Absorbance
Select Test Mode
0
0 2> Single Standard
3> Cut off 0
4> Multi Standard
5> Percent Absorbance
6> Uptake
7> Kinetic
0
0
0
0
D. Enter “Test Name”
NORM – ABSORBANCE
Name :
Pri : 405 Sec : 0
BL N ESC
NORM
MODE
DIFF
NORM – ABSORBANCE
Name:
ABS 560
A B C D E F G H
I J K L M N O P ENTER
Q R S T U V W X CLEAR
Y Z , - + _ 1 2
3 4 5 6 7 8 9 0
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5. Select primary and secondary filter
E. Select primary and secondary filter
NORM – ABSORBANCE
Name: ABS560 BL N ESC
Pri : 405 Sec : 0
NORM
MODE
DIFF
F. If blank is required select ”BL”
NORM – ABSORBANCE
Name : ABS560 BL Y ESC
Pri : 405 Sec : 0
NORM
MODE
DIFF
Pri: 560
Sec: 0
Select Filter Val:
0 405 450
492 630 NA
NA 560 YYY
ESC
If you select “BL Y”, it will read first well first strip ‘A1’ as a blank and subtract the absorbance of blank well from all other well’s absorbance.
G. In absorbance mode there are two modes of operations
Normal Absorbance Mode: Instrument gives the absorbance of each and every well.
Select touch zone “NORM”.
Differential Absorbance Mode: Instrument shows the difference of absorbance between odd number wells and even number wells. For Example,- Strip 1,- Strip 2, - Strip 3, - Strip 4, - Strip
5, -Strip 6. For this Mode select touch zone “DIFF”.
In Normal Mode
NORM – ABSORBANCE
Name: BL N ESC
Pri : 405 Sec : 0 SAVE
NORM
MODE
DIFF
Version 150511
In Differential Mode
DIFF – ABSORBANCE
Name: ESC
Pri : 405 Sec : 0 SAVE
NORM
MODE
DIFF
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8.2. Single Standard
In this mode the instrument accepts the calibrator singly or in duplicate and then calculates the concentration based on the single point standard curve passing through the point 0.000
A single calibrator/standard of a known concentration is used to calibrate the instrument so that the concentration of unknown samples can be calculated according to Beer’s Law. The absorbance are read at user selected wavelengths. If Blank is selected, instrument will automatically blank on the first well and subtract its absorbance from each subsequent well.
The second well is treated as the calibrator/standard well. The third well is also treated as calibrator/standard well if the calibrator/standard is in duplicate.
CALCULATION:
Sample Concentration = (Calibrator concentration /Calibrator Abs.) x Sample Abs
CALCULATION:
Sample Concentration = (Calibrator concentration /Calibrator Abs.) x Sample Abs
Name: SIN_560
Pri: 560 Sec: 0
SINGLE STANDARD
BL Y 0.000 CAL 1 ESC
HI CO : N
DUP N DUP N SAVE
High> Low<
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Entry of all the parameters is similar to Multi standard mode (Please refer Multi standard for entry of parameters)
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8.3. Cut Off Mode
In this mode cut-off point is determined for interpretation of specimens as per formula given in the reagent manual. The negative controls are read followed by the positive controls, Cut-off control, low positive control. Blanking on the first well is optional. The instrument calculates the average of the negative controls and the average of the positive control, Cut-off controls and Low positives are also calculated.
Programming a New Test:
A. Select “Add New Test”
TEST OPERATIONS ESC
1 Select Test By Name
2 Select Test by Mode
3 Select Test by Number
4 List Tests
5 Add New Test
B. Select mode of operation “Cut-Off” in “Add new test”
Select Test Mode
0 1> Absorbance
2> Single Standard
3> Cut off
4> Multi Standard
5> Percent Absorbance
6> Uptake
7> Kinetic
0
0
0
0
0
0
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C. Entry of test name
Select touch Zone “Name:”
Name:
Pri: 450 Sec: 0
No Factor QC
BL 0 N
NC 0 1.000 N
PC 0 1.000 N
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
QCVal Mes. Rem SAVE
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
Alphanumerical characters will appears on the screen as shown below after touching “Name” on the screen. Enter the name of the test by selecting the individual characters one by one. After selecting all the characters select Enter option in touch screen for confirmation of test Name.
Name:
HBSAG
POS- CutOff
A B C D E F G H
I J K L M N O P ENTER
Q R S T U V W X CLEAR
Y Z , - + _ 1 2
3 4 5 6 7 8 9 0
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D. Selection of Primary and secondary filters.
Select zone “Pri:” on the touch screen
Name: HBSAG
Pri: 405 Sec: 0
No Factor QC
BL 0 N
NC 0 1.000 N
PC 0 1.000 N
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
QCVal Mes. Rem SAVE
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
Pri: 405
Sec: 560
Select Filter Val:
0 405 450
492 630 NA
NA 560 YYY
ESC
Select the required filters either Monochromatic or Bichomatic by selecting the filter values from
‘Select Filter Val’ region
E. According to the reagent manual change Blank, NCs, PCs to ‘YES’ for QC check.
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 0 N
NC 0 1.000 N
PC 0 1.000 N
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
QCVal Mes. Rem SAVE
POS- CutOff
ESC
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
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F. Entry of Blank and Blank QC values
Select “BL” to enter blank details.
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 0 Y
NC 0 1.000 Y
PC 0 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.000 0.000
0.000 0.000
0.000 0.000
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
G. After selecting “BL”, enter total number of blanks by selecting No. of Blanks. (Maximum
5 blank).
No. of Blanks = 1
Values Of BL <= 0.000
Numeric Value: 1
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
H. Enter the QC value of the blank by selecting the touch Zone “Values of BL <=”
No. of Blanks = 1
Values Of BL <= 0.000
Numeric Value: 0.100
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
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I. Entry of Negative control and its QC values
Select touch Zone “NC”
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 1 Y
NC 0 1.000 Y
PC 0 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.100
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
Enter N° of Negative controls by selecting touch Zone “N°of NC’s” (maximum 5 Negative
Controls). Enter the QC value of the Negative Control by selecting the touch Zone “Value of
NC” and enter the value of X given in the reagent manual by selecting Zone “Values Of X”.
No. of NC’s = 3
Values Of X = 1.000
Value of NC <= 0.150
Numeric Value
1 2 3 4 5 6 ENTER
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K. After the entry of N° of NC’s Select ESC. The below screen will appear on the display.
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 1 Y
NC 3 1.000 Y
PC 0 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.100
0.150
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
L. Entry of Positive Controls (PC).
Select touch Zone “PC”.
Name: HBSAG
Pri: 450 Sec: 630
No Factor QC
BL 1 Y
NC 3 1.000 Y
PC 0 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.100
0.150
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
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M. After selecting No. of PC’s the screen below will appear on the display. Enter number of
Positive Controls by Selecting Zone “No. of PC’s” (Maximum 5). Enter the QC value of the
Positive Control by selecting the touch Zone “Value of PC >=” and enter the value of Y given in the reagent manual by selecting Zone “Values Of Y”.
No. of PC’s = 2
Values Of Y= 1.000
Value of PC >= 0.500
Numeric Value:
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
N. After entering the details of PCs, escape from the screen by selecting “ESC”. Below screen will appear on the display:
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 1 Y
NC 3 1.000 Y
PC 2 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.100
0.150
0.500
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
NOTE: Same way you can enter values for LC (lower positive controls) and CC (Cut of
Controls).
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O. Entry of Cut off Factor:
Select touch Zone “CUT FAC”
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 1 Y
NC 3 1.000 Y
PC 2 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.100
0.150
0.500
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
After selecting the “CUTFAC” following screen will come on the display:
After selecting the “CUTFAC”, it will display following screen.
ESC
Value of Cut Factor = 0.000
ESC
Value of Cut Factor = 0.200
Numeric Value:
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
Enter the Value for Cut Off Factor and select ESC
option to come out to the test parameter screen.
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P. Entry of Cut off absorbance equation:
Select touch Zone “CUTABS”
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 1 Y
NC 3 1.000 Y
PC 2 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.200
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.100
0.150
0.500
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
After selecting the “CUTABS” below screen will come on the display.
CUTABS: ESC
For entering the CUTABS equation, user has to
CUTABS: ESC
CUT ABS = NC + PC + CF
NC PC LC CC CF ENTER
+ -
1 2
7 8
(
Sqrt Lg ALg
,
3
9
Ln
*
4
0
ALn
%
5
.
Abs
CLEAR
6
) select ESC option in order to come out of the CUTABS screen.
Whereas, NC = Mean of NCs * Factor X Sqrt = Square root
PC = Mean of PCs * Factor Y Lg = LOG Aln = Anti Natural LOG
LC = Mean of LCs * Factor W
CC = Mean of CCs * Factor Z
CF = Cut off Factor
Ln = Natural LOG
Abs. = any absolute value
ALg = Anti LOG
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For Example :
For equation CUT OFF ABS = (0.45 * NC) + (0.35 * PC) + 0.10, the formula can be entered in two ways.
1. Enter the values as it is i.e. CUTABS = (0.45*NC) + (0.35*PC) + 0.010. Keep the multiplication factors X, Y, W, Z of controls NC, PC, CC and LC a constant number ‘01’. (Numbers, mathematical functions and symbols for NC, PC, CC, LPC can be selected from the screen).
OR
2. In second method the formula can be entered as CUT ABS = NC + PC + CF. (Since the Value of
X, Y, Z, W is entered in the QC option of NC, PC, CC, LPC and Cut off factor in the CUT FAC option, there is no need to enter the same data again). Means here Multiplication Factor for NC =
X = 0.45
Multiplication factor for PC = Y = 0.35 and the value of cut of factor = CUT FAC = 0.100.
This is because here NC = Mean of NCs * Factor X, PC = Mean of PCs * Factor Y as mentioned above.
If ‘CUTOFF ABS’ = (NC + PC) / 6. This can be entered as it is CUTABS = (NC + PC) / 6 by keeping the multiplication factors X, Y, W, Z of controls NC, PC, CC and LC a constant number
‘01’.
OR
As you know in mathematics (NC + PC) / 6 = (NC / 6) + (PC / 6) =
(1/6) * NC + (1/6) * PC = (0.166 * NC) + (0.166 * PC),
So, you can enter same equation as CUT ABS = NC + PC, by entering
Multiplication Factor for NC = X = 0.166, multiplication factor for PC = Y = 0.166.
This is because here NC = Mean of NCs * Factor X, PC = Mean of PCs * Factor Y as mention above.
_______________________________________________________________________________
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Q. QC1, QC2, QC3 & QC4. can be used for any QC Checking of the test provided in reagent
manual.
For example some kit may give QC check equation like| PCx – NCx |> 0.500,
PC / NC > 15, etc. This equation can be entered by selecting these QC options. Select touch zone
‘N’. You will obtain ‘Y’, as shown in screen below by selecting touch zone ‘QC1’ and entering
QC equation.
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 1 Y
NC 3 1.000 Y
PC 2 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.200
POS- CutOff
QCVal Mes. Rem SAVE
0.100
0.150
0.500
CUTABS: (
0.45 * NC) + (0.35 * PC) + 0.10
QC1 > Y
QC2 N
QC3 N
QC4 N
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
ESC
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Enter equation of QC and value of QC check by selecting touch zone ‘QC1 Value’ and ‘QC1 equat’.
Enter QC Value and Equation:
QC1 Value: 0.000 ESC
Enter QC Value and Equation:
QC1 Value: 0.500
QC1 Equat :
QC1 Equat :
Numeric Value
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
Enter QC Value and Equation:
QC1 Value: 0.000
QC1 Equat
ESC
QC Equat: PC – NC
NC PC LC CC
* + - ,
1 2 3 4
7 8 9
(
0
Sqrt Log ALog Ln
SA
%
5
.
ALn
ENTER
CLEAR
6
)
Abs
Same way you can enter QC equations for QC2, QC3 and QC4.
*NOTE:
On screen it is mentioned that “QC1 > Y”. In most of the reagent inserts it is mentioned that QC value should be greater than some constant value. Sometimes in the box insert it is mentioned that the value should be less than some constant value. In such case, you can change the symbol from
“QC1 > Y” to “QC1 < Y”, only by selecting a symbol of comparison.
If any QC check is selected to ‘YES’ option, the system will not save the test without entering the
QC equation and QC check value of equation.
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R. Interpretation of samples
Enter RANGE for interpretation of results either in percentage (Greyzone) or positive and negative values of INDEX, as a ratio of (Sample Abs. / Cutoff Abs.). If Cutoff Index range or Greyzone is not mentioned do not enter any values. Instrument will do the interpretation with references to the
Cut off absorbance.
R.1. Interpretation by GREYZONE percentage
Select touch zone ‘Greyzone % (N)’
Select touch zone ‘Greyzone % (N)’
Name: HBSAG
Pri: 405 Sec: 560
No Factor QC
POS- CutOff
ESC
BL 1 Y
NC 3 1.000 Y
PC 2 1.000 Y
QCVal Mes. Rem SAVE
0.100
0.150
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.200
CUTABS: NC + PC + CF
QC1 > Y 0.500
0.500
Interpretation :
QC2 N
QC3 N
QC4 N
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
If you enter ‘Greyzone’ percentage
‘10.00’, instrument will show you the same as shown in screen. It makes the ‘Cutoff Index Range’ option ‘NO’. This means when you are using a ‘Greyzone’ option the
‘Cut off Index Range’ option will not work.
Enter % Value :
% Value = 10.00
Numeric Value :
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
Enter Greyzone percentage value
Select ESC option after entering Greyzone % value to come out to the parameter screen.
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NOTE: If you don’t select any option for interpretation of sample results, the instrument will
take Cutoff Absorbance as a reference.
It will give “Positive’ remark to sample absorbance greater than cutoff and ‘Negative’
to sample absorbance less than cutoff.
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 1 Y
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.100
NC 3 1.000 Y
PC 2 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
0.150
0.500
CUTFAC: 0.200
CUTABS: NC + PC + CF
QC1 > Y 0.500 Interpretation :
QC2 N
QC3 N
QC4 N
Greyzone % (Y) 10.00
Cut off Index (N)
Range
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R.2. Interpretation by ‘Cutoff Index Range”
It is also provided to do the interpretation using ‘Cutoff Index’ by entering normal range for
‘Positive’ and ‘Negative’.
Name: HBSAG
Pri: 405 Sec: 560
No Factor QC
BL 1 Y
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.100
NC
PC
LC
CC
3
2
0
0
1.000 Y
1.000 Y
1.000 N
1.000 N
CUTFAC: 0.200
CUTABS: NC + PC + CF
QC1 > Y 0.500
QC2 N
QC3 N
QC4 N
0.150
0.500
Interpretation :
Greyzone % (N)
Cut off Index (Y)
Range Pos : 0.000
Neg : 0.000
Cut off Index is the ratio of
Sample Absorbance / Cut off
Absorbance.
When you select ‘Cutoff Index’
–‘Y’, the ‘Greyzone %’ will become ‘NO’, means inactive.
In ‘Cutoff Index’, provide the range for interpretation, the positive and negative value of ratio (Sample Abs. / Cutoff Abs.)
RESULT RANGE: ESC
POSITIVE >= 0.000
NEGATIVE <= 0.000
In case of ‘Cutoff Index Range ’,
RESULT RANGE: ESC
POSITIVE >= 1.000
NEGATIVE <= 0.000
Numeric Value
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
It gives the ‘Positive’ remarks to sample having index value, a ratio of …
(Sample abs. / Cutoff Abs.) greater than or equal to entered ‘POSITIVE’ value and it gives ‘Negative’ remarks to sample having index value, a ratio of
(Sample abs. / Cutoff Abs.) less than entered ‘NEGATIVE’ value.
The sample having index value in between ‘POSITIVE’ and ‘NEGATIVE” range get remarks
‘Equivocal’ = ‘EQ’.
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After entering the Interpretation values (ie. conditions for interpretation) the screen will be displayed as follows –
Name: HBSAG
Pri: 405 Sec: 560
No Factor QC
BL 1 Y
NC 3 1.000 Y
POS- CutOff
ESC
QCVal Mes. Rem SAVE
0.100
0.150
0.500 PC 2 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.200
CUTABS: NC + PC + CF
QC1 > Y 0.500 Interpretation :
QC2 N
QC3 N
QC4 N
Greyzone % (N)
Cut off Index (Y)
Range Pos : 1.000
Neg : 0.900
For example:
If you enter POS >= 1.0 and NEG
<=0.9.
Then, the sample will get
POSITIVE remarks having INDEX
Value,
(Sam. Abs./ Cutoff Abs.) >= 1.000.
The sample will get NEGATIVE remarks having INDEX Value,
(Sam. Abs./ Cutoff Abs.) <= 0.900.
The sample will get EQUIVOCAL remarks having INDEX Value,
(Sam. Abs./ Cutoff Abs.) in the range from 0.900 to 1.000.
S. To save the test after entering all the parameters, select ‘SAVE’ option.
Name: HBSAG
Pri: 450 Sec: 560
No Factor QC
BL 1 Y
NC 3 1.000 Y
PC 2 1.000 Y
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.200
POS- Cut Off
ESC
QCVal Mes. Rem SAVE
0.100
0.150
0.500
CUTABS: NC + PC + CF
QC1 > Y 0.500 Interpretation :
QC2 N Greyzone % (Y) 10.00
QC3 N
QC4 N
Cut off Index (N)
Range
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8.4. Reverse Cut Off Mode
For Reverse Cut Off mode, select touch zone ‘POS’- Cut off key. It will change to REV- Cut Off.
Name:
Pri: 450 Sec: 0
No Factor QC
BL 0 N
NC 0 1.000 N
PC 0 1.000 N
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.000
CUTABS:
QC1 N
QC2 N
QC3 N
QC4 N
REV- CutOff
ESC
QCVal Mes. Rem SAVE
Interpretation :
Greyzone % (Y) 10.00
Cut off Index (N)
Range
In Reverse Cut Off Mode, programming of test is the same as Positive Cut off Mode. Only QC check conditions for blanks and controls get reversed. In the ‘INTERPRETATION’, sample absorbance, which is lesser than cut off absorbance, gets remarked POSITIVE . Sample absorbance higher than cut off absorbance, gets remarked NEGATIVE. This is exactly the opposite to normal
POSITIVE Cut Off Mode. Same way in case you select ‘Cutoff Index Range’.
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8.5. Multi Standard Mode:
In this mode the instrument accepts a maximum of 12 calibrators and calculates concentration based
on the best-fit curve. Graph is printed with Absorbance on Y-axis and concentration on Xaxis.
Programming a New Test:
A. Select Main Menu/Test Operation/Add New Test B. Select Touch Zone “Multi Standard”
TEST OPERATIONS
1 Select Test By Name
2 Select Test by Mode
3 Select Test by Number
4 List Tests
5 Add New Test
ESC
Select Test Mode ESC
0 1> Absorbance
2> Single Standard
3> Cut off
0
0
4> Multi Standard
5> Percent Absorbance
6> Uptake
7> Kinetic
0
0
0
0
After selecting Multistandard mode it will display following screen -
Name: MULTISTANDARD
Pri: 405 Sec: 0 BL N
DUP N
CAL 2 ESC
DUP N
HI CO : N High> Low<
SAVE
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Y vs X : ABS vs CONC
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C. Entry of Test Name
Test Name can be entered by Selecting Touch Zone “Name:”
Procedure for entering the test name is the same as the one of cut off mode. Refer Test Name entry in Cut off mode for further details.
D. Selection of Primary and secondary filters
Select Touch Zone “Pri:”
Name: MUL_4P MULTISTANDARD
Pri: 405 Sec: 0 BL N CAL 2 ESC
DUP N DUP N
SAVE
HI CO : N High> Low<
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Y vs X : ABS vs CONC
Pri: 405
Sec: 0
Pri: 560
Sec: 0
Select Filter Val:
0 405 450
492 630 NA
NA 560 YYY
ESC
ESC
Select the Filters as per details given in the reagent manual
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E. Selection of Blank
Select Touch Zone “BL”
Select Touch Zone “BL”. Instrument will display the message “Blank required Yes/No” at the bottom of the Screen, select “Yes” if blank is required.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL N CAL 2 ESC
DUP N DUP N
HI CO : N High> Low<
SAVE
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Y vs X : ABS vs CONC
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL N CAL 2 ESC
DUP N
HI CO : N High> Low<
DUP N
SAVE
LO CO: N High> Low<
Interpret’n : N Range High>
Low<
Graph : LINEAR
Blank Required ? YES / NO
F. Entry of Standard Concentration
Select touch Zone “CAL” to enter Number of calibrators and its concentration.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 2 ESC
DUP N DUP N
SAVE
HI CO : N High> Low<
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Y vs X : ABS vs CONC
After selecting “CAL”, it will display following screen:
Select Touch Zone “No. Of Calibrators”. Numeric screen will be displayed at the bottom of the screen.
Select the number of calibrators (User can select min 2 and max. 12 standard in this mode).
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Whereas in case of 4 Parameter, user can select min. 5 and max.12 calibrators.
No Of Calibrator: 2
No Conc Abs
1 0.000 0.000
2 0.000 0.000
ESC No Of Calibrator: 2
No Conc Abs
1 0.000 0.000
2 0.000 0.000
Numeric Value
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
ESC
Concentration Entry:
After entering the number of calibrators, concentration and absorbance column will come on the display as shown below.
To enter concentration values select 0.000 in Conc. column corresponding to the standard and enter the concentration value. e.g. for entering Concentration of standard 4 Select 0.000 in fourth row of
Conc. Column and enter the concentration by selecting the numerals.
(Note: The concentration value should be either in ascending or descending order.)
No Of Calibrator 7 ESC
No Conc Abs No Conc Abs
1 0.000 0.000 7 10.00 0.000
2 0.500 0.000
3 1.000 0.000
4 2.500 0.000
5 5.000 0.000
6 7.500 0.000
Numeric Value
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
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Screen after entry of calibration will be displayed as follows -
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP N DUP N
SAVE
HI CO : N High> Low<
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Y vs X : ABS vs CONC
G. Selection of Duplicate Blank and calibrators
For Duplicate Blank:
Select touch zone “DUP” below the “BL Y” After selecting “DUP” instrument will display message “Duplicate Blank? Yes/ No” as shown below. Select “Yes” for Duplication of Blank.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP N DUP N
HI CO : N High> Low<
SAVE
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Y vs X : ABS vs CONC
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP N DUP N
HI CO : N High> Low<
SAVE
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Duplicate Blank?
YES / NO
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For Duplicate Calibrators:
To select Duplicate Calibrator select Touch Zone “DUP” below the “CAL 7”. After selecting
“DUP” instrument will display message “Duplicate Calibrator? Yes/No” as shown below. Select
“Yes” for Duplication of calibrators.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y
HI CO : N High> Low<
DUP N
SAVE
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Y vs X : ABS vs CONC
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y DUP N
HI CO : N High> Low<
SAVE
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Duplicate Calibrator?
YES / NO
Screen after standard entry & selection of duplicate – Blank & Calibrators.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y DUP Y
SAVE
HI CO : N High> Low<
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Y vs X : ABS vs CONC
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H) Graph type along with its scales:
There are three types of graphs in Multistandard. They are mentioned as follows -
1. Linear (LINEAR)
2. Point to Point (PT TO PT)
3. 4 Parameters
There are five types of scales for X and Y axis
LOGABS vs CONC (X-axis = Concentration v/s Y-axis = LOG of ABS.)
ABS vs LOGCONC (X-axis = LOG of concentration v/s Y-axis = Absorbance)
LOGITABS VS LOGCONC (X-axis = LOG of concentration v/s Y-axis = LOGIT of Absorbance)
ABS vs CONC (X-axis = Concentration v/s Y-axis = Absorbance)
LOGABS vs LOGCONC (X-axis = LOG of concentration v/s Y-axis = LOG of absorbance)
Selection of Graph
Select Graph type as per the details given in the reagent manual by selecting Touch Zone “Graph”.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y DUP Y
HI CO : N High> Low<
SAVE
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : LINEAR
Y vs X : ABS vs CONC
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1. Linear mode graph type:
After Selecting the “Graph” follow screen will come on the display.
Select Graph type as Linear.
Select scale as per the details given in the reagent manual.
Graph : LINEAR ESC
Y vs X : ABS vs CONC
TYPE
LINEAR
SCALE
LOGABS vs CONC
ABS vs LOGCONC
PT TO PT LOGITABS vs LOGCONC
ABS vs CONC
4 PARAMETER LOGABS vs LOGCONC
2. Point to Point Graph Type:
Select touch zone “PT to PT” abs scale of graph ‘ABS vs. CONC’.
Graph Type : PT TO PT
Y vs X : ABS vs CONC
TYPE SCALE
LINEAR LOGABS vs CONC
PT TO PT
ABS vs LOGCONC
LOGITABS vs LOGCONC
ABS vs CONC
4 PARAMETER LOGABS vs LOGCONC
ESC
3. 4 Parameters Graph Type:
Select touch zone “4 Parameters” & abs scale of graph as ‘ABS vs. CONC’. Remember that it's default scale type is “ABS vs CONC”.
Graph Type : 4 PARAMETER
Y vs X : ABS vs CONC
TYPE SCALE
ESC
LINEAR LOGABS vs CONC
ABS vs LOGCONC
PT TO PT LOGITABS vs LOGCONC
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ABS vs CONC
4 PARAMETER LOGABS vs LOGCONC
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Select the scale type touching the required zone.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y DUP Y
SAVE
HI CO : N High> Low<
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : 4 PARAMETER
Y vs X : ABS vs CONC
I. Selection of Controls
For High control select touch zone ‘HI CO: N’ Following message appears: “High Control
Required? Yes / No”. Select ‘YES’ and then enter range of control “HIGH” and “LOW”.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y DUP Y
HI CO : N High> Low<
SAVE
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : 4 PARAMETER
High Control Required
?
YES / NO
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Enter range of control “HIGH” and “LOW” as per reagent manual.
Enter High Range Value:
High 0.000
Low 0.000
ESC Enter High Range Value:
High 25.03
Low 10.54
ESC
Numeric Value
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
Similarly, for Low Control select touch zone ‘LO CO: N’ It will display the message “Low Control
Required? Yes / No”. Select ‘YES’ and then enter range of control “HIGH” and “LOW”.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y DUP Y
SAVE
HI CO : N High> 25.03 Low< 10.54
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
Graph : 4 PARAMETER
Low Control Required
?
YES / NO
(Note: Entry of LO CO is similar to that of HI CO)
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J. Normal Range selection for interpretation of samples:
For interpretation of specimen result (POSITIVE or NEGATIVE), enter normal range given in
REAGENT MANUAL. Select “Interpretation: N”. It will change to “Interpretation: Y”. Then select “HIGH>” and “LOW<” to enter normal range.
Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y DUP Y
SAVE
HI CO : Y High> 25.03 Low< 10.54
LO CO: Y High> 16.61 Low< 5.45
Interpret’n: Y Range High> 0.000
Low< 0.000
Graph : 4 PARAMETER
Y vs X : ABS vs CONC
All the results whose concentration is coming above the High Range will be considered as positive and coming below the Low Range will be consider as negative.
The results having concentration in between High and Low range will get remarks ‘Equivocal’ = ‘EQ’.
Enter Normal Range for positive and negative samples.
Enter HIGH Range value:
High> 0.000
Low< 0.000
ESC
Enter HIGH Range value:
High> 15.25
Low< 9.46
Numeric Value
ESC
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
After entering all the parameters save the test by selecting “SAVE” option.
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Name: MUL_4P MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y DUP Y
SAVE
HI CO : Y High> 25.03 Low< 10.54
LO CO: Y High> 16.61 Low< 5.45
Interpret’n: Y Range High> 15.25
Low< 9.46
Graph : 4 PARAMETER
Y vs X : ABS vs CONC
(*Same way you can make a test for Single standard, % Absorbance and uptake)
8.6. Percentage Absorbance
The Percentage Absorbance Mode requires one calibrator (read singly or duplicate). In this mode, calibrator is considered to have a concentration of 100%. The absorbance’s of unknown samples are read and compared to the calibrator absorbance, and reported as % concentration of calibrator.
Refer 8.5 for parameter entry.
Name: % ABSORBANCE
Pri: 405 Sec: 0 BL Y 0.000 CAL 1 ESC
DUP N DUP N
SAVE
HI CO : N High> Low<
LO CO: N High> Low<
Interpret’n: N Range High>
Low<
8.7. Uptake
In this mode the instrument accepts the calibrator singly or in duplicate and then calculates the concentration based on the single point standard curve passing through the point 0.000
A single calibrator/standard of a known concentration is used to calibrate the instrument so that the concentration of unknown samples can be calculated according to Beer’s Law
Sample Concentration = Calibrator Absorbance * Calibrator Concentration
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Sample Absorbance
Name: UPTAKE
Pri: 405 Sec: 0 BL N CAL 1 ESC
DUP N DUP N
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LO CO: N High> Low<
Interpret’n: N Range High>
Low<
8.8.
Kinetic
After entering Test Name and Filter values, select Read Time and Read Period
KINETIC
Name: KIN_560 ESC
SAVE Pri: 560 Sec: 0
Read Time: 1 min.
Read Period: 15 sec.
KINETIC
Name: KIN_560
Pri: 560 Sec: 0
Read Time: 1 min.
ESC
SAVE
KINETIC
Name: KIN_560
Pri: 560 Sec: 0
Read Time: 1 min.
Read Period: 15 sec.
Numeric Value
ESC
SAVE
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
“Read Time” is total read period of the whole test.
You need to enter it only in minutes.
Read Period: 15 sec.
Numeric Value
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
“Read Period” is time interval between two readings.
User must enter it in seconds.
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Finally select the “Save” option in order to save the test in KINETIC mode
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Name: KIN_560
KINETIC
Pri: 560 Sec: 0
Read Time: 1 min.
Read Period: 15 sec.
ESC
SAVE
9. RECALLING AND RUNNING OF STORED
TEST/PROGRAMS
MAIN MENU
TEST OPERATIONS
1 Test Operations ESC
1 Select Test By Name
2 Technical Diagnosis
2 Select Test by Mode
3 Utilities
3 Select Test by Number
4 View Plate
4 List Tests
5 Serial Communication
5 Add New Tests
Test can be recalled by Name, Mode, Number or by List test.
ESC
The entire saved tests are available on the List Test or on the first screen after initialization.
User can directly RUN the test by selecting RUN option present on the screen.
TEST OPERATIONS
1 Select Test By Name ESC
2 Select Test By Mode
3 Select Test By Number
4 List Tests
5 Add New Tests
Select Test
1 HBSAG
ESC
RUN / SEL
2 TSH PRNT
3 HCV NXT
4 MUL_4P PREV
5 CP_560 DEL
PLATE
DATA
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Tests can be selected with the help of SEL option present on the Select Test screen in order to
EDIT / PRINT the test parameters entered before saving the test.
Select Test ESC
1 HBSAG RUN / SEL
2 TSH PRNT
3.HCV NXT
4 MUL_4P PREV
5 CP_560 DEL
PLATE
DATA
NORM – ABSORBANCE
Name : HCV BL N ESC
EDIT Pri : 450 Sec : 560
NORM
MODE
RUN
DIFF PRINT
9.1. Absorbance Mode:
Select the test and point to RUN option
TEST OPERATIONS
1 Select Test By Name ESC
2 Select Test By Mode
3 Select Test By Number
4 List Tests
5 Add New Tests
On selecting RUN option it will display following screen.
Select Test
1 HBSAG
ESC
M
2 TSH S
RUN / SEL
PRNT
3. HCV A NXT
4 MUL_4P M PREV
5 CP_560 C DEL
PLATE
DATA
The instrument will ask “Is Plate Loaded? YES / NO”, select ‘Yes’. Instrument will read the absorbance using mechanical plate movement. After completion of reading it will ask:
“Is Plate Removed? YES / NO”. Remove the plate and Select ‘YES’.
Name: HCV NORM – ABSORBANCE
Pri : 450 Sec : 560 BL N
ABS ABS ABS ABS ABS ABS
1 2 3 4 5 6
ABS ABS ABS ABS ABS ABS
7 8 9 10 11 12
Is Plate Loaded ? YES / NO
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Name: HCV NORM – ABSORBANCE
Pri : 450 Sec : 560 BL N
ABS ABS
1 2
ABS
3
ABS
4
ABS
5
ABS
6
ABS ABS ABS ABS ABS ABS
7 8 9 10 11 12
Is Plate Removed ? YES / NO
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Details of absorbance of all wells in table format for each strip will be displayed on the screen as shown below.
In this operation if you keep BLANK
Name: HCV NORM – ABSORBANCE
Pri : 450 Sec : 560 BL N
ABS ABS ABS ABS ABS ABS
1 2 3 4 5 6
0.028 0.145 0.100 0.017 0.089 0.135
-- --
--
--
--
0.071 0.123 0.113 0.054 0.116 0.060
ABS ABS ABS ABS ABS ABS
7 8 9 10 11 12
0.362 0.078 0.410 1.440 0.746 0.224
-- --
--
--
--
0.112 0.111 0.093 2.568 0.396 0.015
ESC PRINTMATRIX SEND PLATE LOAD NEXT
YES
The first well of first strip will be considered as blank and instrument will give the absorbance of remaining all well with blank subtraction from original absorbance.
The “PRINT MATRIX” will print the details of entire plate with well identification and absorbance of that well, like…..
A
W1 W2 W3 W4 W5 W6
0.125 0.250 0.350 0.450 0.550 0.650..
Using “SEND PLATE” plate data can be sent to computer using either USB or
SERIAL RS232.
“LOAD NEXT” is used to load the Next
Plate in order to get the absorbance of next plate
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Example Print-out of ABSORBANCE test obtained from ELISA Reader
After running the plate in Absorbance mode, user can take the print of the result in the form of
Print matrix which will be printed as follows –
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Differential Absorbance Mode: In differential absorbance mode the instrument will give the absorbance difference each set of “ODD” number strip and EVEN number strip.
Name: ABS DIFF– ABSORBANCE
Pri : 560 Sec : 0
ABS ABS ABS ABS ABS ABS
1 2 3 4 5 6
0.900
0.200
0.300
1.700 0.900
1.000 1.800
1.100 1.900
0.400
0.500
0.600
0.700
1.200
1.300
1.400
1.500
2.000
2.100
2.200
2.300
0.800 1.600 2.400
ABS ABS ABS ABS ABS ABS
7 8 9 10 11 12
0.100
0.200
0.300
0.400
0.500
0.600
0.700
0.800
0.900
1.000
1.100
1.200
1.300
1.400
1.500
1.600
1.700
1.800
1.900
2.000
2.100
2.200
2.300
2.400
ESC PRINTMATRIX SEND PLATE LOAD NEXT
It is showing the absorbance only for even number strips. This absorbance values are actually differences between each well of strip 1 and well of strip
2.You can get the difference between set of 1-2, 3-4, 5-6, 7-8, 9-10 and 11-12. It is not possible to change this combination.
In “PRINT MATRIX”, you will get the print of entire plate data with actual absorbance of each well and difference of absorbance between ODD well and
EVEN well, like
A
W1 W2 W3 W4 W5 W6 W7 W8 W9 W10 W11 W12
0.300 0.279 0.524 0.497 0.816 0.499 0.082 0.474 0.651 0.331 0.394 0.562
0.000 0.021 0.000 0.028 0.000 0.316 0.000 -0.391 0.000 0.320 0.000 -0.168
B
W1 W2 W3 W4 W5 W6 W7 W8 W9 W10 W11 W12
0.506 0.584 0.648 0.194 0.472 0.457 0.822 0.346 0.679 0.387 1.047 0.130
0.000 -0.077 0.000 0.453 0.000 0.015 0.000 0.476 0.000 0.292 0.000 0.918
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9.2. Cut off Mode
After recalling the test saved in Cut-Off mode, it will display following screen.
User can take the print of the test parameters by selecting “PRNT” option present on the
screen. It will print all the details of the test parameters alongwith QC equations.
Name: HCV POS- Cut Off
Pri: 560 Sec: 0 ESC
No
BL 1
NC 2
Factor QC
Y
1.000 Y
PC
LC
2
0
1.000 Y
1.000 N
CC 0
CUTFAC: 0.100
1.000 N
QCVal Mes.
0.100
0.150
0.000
0.000
0.500 0.000
CUTABS: 0.1*PC+CF
QC1 N
QC2 N
Interpretation :
Greyzone % (Y) 10.00
QC3 N
QC4 N
Cut off Index (N)
Range
To change values press EDIT
EDIT
RUN
PRNT
A) For running the test in CUTOFF mode select the particular test and point to RUN option
present on the “List Test” screen.
Select Test ESC
1 HBSAG M RUN / SEL
2 TSH S PRNT
3 HCV C NXT
4 MUL_4P M PREV
5 CP_405 C DEL
PLATE
DATA
NC
NC
PC
PC
Name: HCV
Pri: 560 Sec: 0
No. of Samples: 0
POS- Cut Off
ESC
RUN
DUP N SIMUL PID
1 2 3 4 5 6 7 8 9 10 11 12
B
NC
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B) It shows the 12 strips horizontal with 8 wells vertical with blanks and controls loaded in first strip. Select the ‘No. of Samples’ and enter the total Number of samples you want to load with the help of numeric screen displayed at the bottom of the screen.
Name: HCV
Pri: 560 Sec: 0
No. of Samples: 0
DUP N SIMUL
POS- Cut Off
ESC
PID
RUN
1 2 3 4 5 6 7 8 9 10 11 12
Name: HCV
Pri: 560 Sec: 0
No. of Samples: 10
DUP N SIMUL
POS- Cut Off
ESC
RUN
PID
1 2 3 4 5 6 7 8 9 10 11 12
B S3
NC S4
B
NC
NC
NC
PC
PC
Numeric Value: 10
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
NC S5
NC S6
PC S7
PC S8
S1 S9
S2 S10
C) After entering No. of samples, select RUN. The plate-loading tray will come out and the following message will appear: “Is plate loaded? YES / NO”
Name: HCV
Pri: 560 Sec: 0
No. of Samples: 10
DUP N SIMUL
POS- Cut Off
PID
ESC
RUN
1 2 3 4 5 6 7 8 9 10 11 12
B S3
NC S4
NC S5
NC S6
PC S7
PC S8
S1 S9
S2 S10
Is Plate Loaded ? YES / NO
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D) Here the plate is loaded in the direction that controls should go in first. Select “YES”.
The instrument will read the controls and samples.
Following message appears: “Is plate removed? YES / NO”. Remove the plate and select YES.
Name: HCV
Pri: 560 Sec: 0
POS- Cut Off
No. of Samples: 10
DUP N SIMUL PID
ESC
RUN
1 2 3 4 5 6 7 8 9 10 11 12
B S3
NC S4
NC S5
NC S6
PC S7
PC S8
S1 S9
S2 S10
Is Plate Removed? YES / NO
E) After selecting YES, result screen will appear with the measured values of average blank, average of all controls and the cut off absorbance. To check the results of samples, select option ‘Display value’
Name: HCV
Pri: 560 Sec: 0
No Factor QC
POS- Cut Off
ESC
QCVal Mes. Rem ACCEPT
0.100 0.050 TEST BL 1 Y
NC 2 1.000 Y
PC 2 0.100 Y
0.150 0.098
0.500 2.658
LC 0 1.000 N
CC 0 1.000 N
CUTFAC: 0.200
QC1 2.560
Range % 10.00
QC2
QC3 QC4
CUTABS: 0.563
Display
VALUE
LOAD
NEXT
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F) ‘DISPLAY VALUE' option will display the result of all samples along with the controls
selected.
Name: HCV POS- Cut Off
Pri: 560 Sec: 0 BL Y
Range PERCENTAGE % 10.00
ESC
NXT
Pos: 0.330
CUT ABS= 0.300 Neg: 0.270 PREV
NO SAMP ABS AVG INDEX REM PID
A1 B 0.050 0.050
B1 NC
--
--
G1 S1 0.200 0.200 0.254 NEG PQR-1
H1 S2 1.854 1.854 7.245 POS PQR-2
RESULTS
SAVE
RESULTS
MATRIX
PLATE
OPT.
G) By selecting ‘ACCEPT TEST’ option you can save this test with the details of controls absorbance. Next time it is possible to run the same test without loading controls. This means that the previous stored values of controls can be used.
All the details are in table format, as per row and column shown on the screen. You can obtain the print of the same format by selecting option ‘PRINT RESULTS’. To check the results of next samples, choose option ‘NXT’, and then print it in format as shown on the screen by option ‘Print
Results’
You can obtain the print in matrix format, by selecting option ‘PRINT MATRIX’.
In matrix form you will get the print as per your tray/plate for all the wells. Instrument prints seven different parameters in print matrix for a single well.
ROW IDENTIFICATION
WELL NUMBER
WELL ID OR SAMPLE NUMBER
PATIENT IDENTIFICATION
WELL ABSORBANCE
SAMPLE CONCENTRATION
INTERPRETATION / REMARKS
A, B, C,D,……
W1, W2, W3, W4…..
BL, NC, PC, OR S1, S2, S3, …..
RAMESH, VIKAS, etc.
0.050, 0.098, 0.085, …
0.738, 0.689, 2.578,…
POS or NEG or EQ
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Example Print-out of POS-CUTOFF test obtained from ELISA
Reader
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9.2. A. Invalid Assay in Cut Off Mode
If any individual control behaves incorrectly and it’s absorbance is not satisfying the QC check value of that control, the following message appears: “Invalid Assay” and a remark “HI” or “LO” for that particular control. OR if the QC1, QC2, QC3 and QC4, which has an other QC condition of controls (like some reagent manual gives condition PC-NC > 0.2 or NC/PC > 0.5) the following message appears: “Invalid Assay” and a remark either ‘HI’ or ‘LO’ for that QC condition.
Name: HBSAG
Pri: 560 Sec: 0
No Factor QC
BL 1 Y
POS- Cut Off
ESC
QCVal Meas. Rem ACCEPT
0.100 0.105 HI TEST
NC 3 1.000 Y
PC 2 0.100 Y
LC 0 1.000 N
Invalid Assay
0.150 0.200 HI
0.500 0.456 LO
CC 0 1.000 N
CUTFAC: 0.200
QC1 2.560 HI QC2
CUTABS: 0.563
QC3 QC4
DISPLAY
VALUE
LOAD
NEXT
(NOTE: The absorbance of controls should not go above
3.50. If it is > than 3.50, it will show the remark ‘HI’ for that control. In such a case edit the control absorbance.)
In such case, delete an individual control, so that the average of remaining controls will satisfy the
QC condition of that control as per reagent manual.
To select any control for deleting, you must select the touch zone as shown in the above screen.
When you select any particular control for deleting (suppose you have selected Negative Control
(NC), ‘HI’ or ‘LO’ remark appear on the screen.
“Select EDIT / DEL key to proceed”. To delete the control select ‘DEL’. After deleting that control, the absorbance of that control will become zero and the average of controls will also get changed. Select ‘ESC’ option to come back to the Result screen.
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NC VALUE :
NC1 0.150
0.200
NC2 0.350 HI
NC3 0.200 HI
ESC
DEL
When you go back, you will get the edited values for average of control with no remark for any control and no “Invalid Assay” message.
*NOTE:
Make the blank absorbance valid in case the absorbance doesn’t meet the QC conditions.
If necessary valid the control absorbance because the absorbance of the blank affect the absorbance of all controls and samples.
It is recommended that, for a test with single blank and single control, if absorbance doesn’t satisfy
QC conditions or if all controls of any single type (means all NC controls or all PC controls) doesn’t meet the QC, the test will become totally invalid. It is not possible to make the VALID
TEST and new controls have to be loaded.
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9.3. Multistandard
After recalling the test in multistandard mode it will display following screen. Take the print of the
test parameters if required and select “Run” option. Or else you can directly run the test from the
List Test screen.
Multi Standard Mode
Name: TSH MULTISTANDARD
Pri: 450 Sec: 560 BL Y 0.000 CAL 7 ESC
DUP Y DUP Y EDIT
RUN
HI CO: Y High> 25.03 Low< 10.54 PRINT
LO CO: Y High> 16.61 Low< 5.45
Interpret’n: Y Range High> 15.25
Low< 9.46
Graph: 4 PARAMETER
Y vs X : ABS vs CONC
To change values press EDIT
A) For running the test in MUL_STD mode select the particular test and point to RUN option present on the screen. On selecting RUN option it will display following screen.
Select Test
1. HBSAg
ESC
2. HCVAb M RUN / SEL
3. UPT
4. TSH
5. CPC C
6. PER_ABS P NEXT
7. CRP C
8. KIN_560 K PREV
9. SIN_630 S
10. CP_405 C DEL
PLATE
DATA
Name: TSH
Pri: 450 Sec: 560
No. of samples: 0
MULTISTANDARD ESC
RUN
DUP N SIMUL PID
1 2 3 4 5 6 7 8 9 10 11 12
B
C1
C2
C3
C4
C5
C6
C7
HI CO: 0 LO CO: 0
COL COL
ROW ROW
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B) To enter total No. of HI controls, select “HI CO:” option and enter numeric value.
Numeric Value
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
C) If you select No. of HI controls = 2, then you have to locate the position of HI controls by selecting column number and row number.
For column number entry select “COL” option and enter column number.
HI CO : 2
ROW COL
2
3
Please Enter Col Entry: 1
Numeric Value:
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
For row entry select “ROW” option and enter row position.
HI CO : 2
ROW COL
A 2
B 3
Please Enter Row Entry: 1
Row Entry:
A B C D E F G H ENTER
CLEAR
Similarly, you can enter No. of LO Controls and locate its positions.
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D) You can locate Controls from first well or after last calibrator to last well of strip 12 in any test.
Suppose you select column 2 & row A for first HI control and column 2, row B for second HI control then the location of HI control will be display on screen as shown below.
Name: TSH
Pri: 450 Sec: 560
No. of samples: 0
DUP N SIMUL
MULTISTANDARD
PID
ESC
RUN
1 2 3 4 5 6 7 8 9 10 11 12
B HC
C1 HC
C2
C3
C4
C5
C6
C7
HI CO: 2
COL
ROW
E) Select No. of samples you want to run. In case of 10 samples, it will load 10 samples
as follows -
Name: TSH
Pri: 450 Sec: 560
No. of samples : 10
DUP N SIMUL
MULTISTANDARD
PID
ESC
RUN
1 2 3 4 5 6 7 8 9 10 11 12
B HC S5
C1 HC S6
C2 LC S7
C3 LC S8
C4 S1 S9
C5 S2 S10
C6 S3
C7 S4
HI CO: 2
COL
ROW
LO CO: 2
COL
ROW
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F) Now Select RUN option so that readings can be taken in MULTI STANDARD mode.
The plate holder will come out and will display the message string- “Is Plate loaded YES / NO”.
The plate has to be loaded in such a way that the direction of the calibrators should go in first and then select ‘YES’.
The instrument will read the absorbance of calibrators and samples. And finally display the string-
“Is plate removed? YES / NO”. Remove the plate and select ‘YES'. The plate holder will go inside and following screen will be flashed on the display.
(Note: It takes maximum 6 min only in case of 4 parameter)
Name: MULTI STANDARD ESC
Pri: Sec: BL CAL NXT
DUP DUP PREV
Range High > Low <
HI CO : High > Low < SAVE
LO CO : High > Low < RESULT
NO SAMP ABS AVG CONC REM PID
Please Wait…………
It may take maximum 6 minutes.
LOAD PRINT PLATE VIEW ACCEPT PRINT
NXT MATRIX OPT. GRAPH TEST RESULTS
After reading it will display all the details of calibrators, samples and controls on the screen as shown below-
Name: TSH
Pri: 450 Sec: 560
MULTI STANDARD
BL Y CAL 7
ESC
DUP N DUP N
Range High > 15.25 Low < 9.46 NXT
HI CO : High > 25.03 Low < 10.54
LO CO : High > 16.61 Low < 5.45 PREV
NO SAMP ABS AVG CONC REM PID
A1 B 0.050 0.050
B1 C1 0.070 0.070 0.000
C1 C2 0.257 0.257 2.500
--
SAVE
RESULT
--
--
G1 S1 0.200 0.200 2.100 NEG TSH-1
H1 S2 1.854 1.854 9.845 POS TSH-2
LOAD PRINT PLATE VIEW ACCEPT PRINT
NXT MATRIX OPT. GRAPH TEST RESULTS
All the details as per row and column are shown on the screen. The printout of the same format can be obtained by selecting ‘PRINT RESULTS’. To check the results of next samples, select
‘NXT’ and print the format shown on screen by option ‘Print Results’.
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You can get the print in matrix format, by selecting ‘PRINT MATRIX’.
In matrix form you will get the print as per your plate for all the wells. Instrument will print seven different parameters in print matrix for a single well as follows:
ROW IDENTIFICATION
WELL NUMBER
WELL ID OR SAMPLE NUMBER
PATIENT IDENTIFICATION
WELL ABSORBANCE
SAMPLE CONCENTRATION
INTERPRETATION / REMARKS
A,B, C,D,……
W1, W2, W3, W4…
B, C1, C2, C3, OR S1, S2, S3,..
LUCAS, DUPONT, etc.
0.050, 0.098, 0.085, …
0.738, 0.689, 2.578…
POS, NEG, EQ,
Remember that Negative sign result is interpreted as zero both in printout as well as on screen.
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Example Print-out of MultiStandard test obtained from ELISA Reader
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G) By selecting option ‘View Graph:’ key, the graph appears on the screen, with all the details of X-axis and Y-axis. Select ‘PRINT’ option to obtain the print out.
Origin at : X =0.000 , y = 0.000
X / div = 2.500 , y / div = 0.150
3.000
+
ESC
2.000
+
EDIT
+
1.000
+
+
0.001
+ +
0.000 10.00 20.00 40.00
Select ‘ACCEPT TEST’ option to save this test with the details of calibrator absorbance. It is possible to run the same test without loading calibrators. The previous graph can be used for new samples.
(* NOTE: If you do not want to run the calibrators each time, please select “ACCEPT TEST” option to save graph, whenever you run the test with calibrators.)
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9.3.A. Invalid assay in Multi standard Mode
In Multi standard, the absorbance of calibrators should be in increasing or decreasing from one calibrator to the next calibrator. If any calibrator/s behave(s) incorrectly you will obtain a message
“Invalid Assay” at bottom side of screen.
Name: TSH
Pri: 450 Sec: 630
Range High > 16.61
MULTI STANDARD
BL Y CAL 7
Low < 10.30
ESC
NXT
HI CO : High >
LO CO : High >
Low < 0.000
Low < PREV
NO SAMP ABS AVG CONC REM PID
A1 B 0.050 0.050
B1 C1 0.070 0.070 0.000
SAVE
RESULT
C1 C2 0.257 0.257 2.500
---
---
---
Invalid Assay
LOAD PRINT PLATE
NXT MATRIX OPT.
VIEW
GRAPH:
ACCEPT PRINT
TEST RESULTS
If Assay is Invalid then “Accept Test”, “Save Results”, “Print Matrix”, “Save Plate” options are blocked. In such a case, select VIEW GRAPH option and change the ASSAY to VALID with the help of EDIT option present on the Graph screen.
CAL 1
CAL 2
CAL 3
CAL 4
CAL 5
CAL 6
CAL 7
2.562
1.865
2.050
1.054
0.648
0.356
0.045
ESC
CAL1 2.562
CAL 2 2.259
2.050 CAL 3
CAL 4 1.054
CAL 5
CAL 6
0.648
0.356
CAL 7 0.045
Numeric Value: 2.250
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
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You can easily select any particular calibrator by touching that touch zone and edit the corresponding absorbance . After editing the calibrator’s absorbance select “ESC”. A message will appear: “PLEASE WAIT…” and the modified graph will appear on the screen.
If calibrator's value being edited in Multistandard mode then "*Mod…" string is displayed in the print.
9.4. Kinetic:
Select “RUN” to start to run the test in Kinetic mode.
Name : KIN_560
Pri : 560 Sec : 0
Read Time : 1 min.
.
Read Period : 15 sec.
KINETIC
ESC
EDIT
RUN
Or else you can directly run the test from the List Test screen by selecting RUN option present on the screen.
Select Test
1. HBSAg
ESC
2. HCVAb M RUN / SEL
3. UPT
4. TSH
5. CPC C
6. PER_ABS P NEXT
7. CRP C
8. KIN_560 K PREV
9. SIN_630 S
10. CP_405 C DEL
PLATE
DATA
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The “D/M” means delta per minute of reaction. When test will over, instrument will show you the
“D/M” values of reaction done in each well.
Name: KIN_560
Pri : 560 Sec : 0
KINETIC
D/M D/M D/M D/M D/M D/M
1 2 3 4 5 6
D/M D/M
7 8
D/M
9
D/M D/M D/M
10 11 12
Is Plate Loaded ? YES / NO
Name: KIN_560
Pri : 560 Sec : 0
KINETIC
D/M D/M D/M D/M D/M D/M
1 2 3 4 5 6
D/M D/M D/M D/M D/M D/M
7 8 9 10 11 12
Is Plate Removed ? YES / NO
It is showing the “D/M” values for each and every well in table format.
Name: KIN_560 KINETIC
Pri : 560 Sec : 0
D/M D/M
1 2
D/M
3
D/M
4
D/M
5
D/M
6
0.020 0.002 0.010 0.035 0.056 0.001
0.030 0.056 0.075 0.100 0.068 0.090
-- --- --- --- --- ---
-- --- --- --- --- ---
D/M D/M
7 8
D/M
9
D/M
10
D/M
11
D/M
12
0.020 0.002 0.010 0.0.35 0.056 0.001
0.030 0.056 0.075 0.100 0.068 0.090
--- --- --- --- --- ---
ESC PRINTMATRIX SEND PLATE LOAD NEXT
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In “PRINT MATRIX”, you will get the print of entire plate data with initial absorbance of each sample, average delta values of each sample and “D/M” values of each well, like…
A
W1 W2 W3 W4 W5 W6
0.200 0.100 1.200 0.300 2.600 0.900.. these are initial absorbance.
0.010 0.001 0.005 0.017 0.028 0.001.. these are average delta values.
0.020 0.002 0.010 0.035 0.056 0.001.. these are “D/M” values of each sample.
B
W1 W2 W3 W4 W5 W6
0.300 0.100 1.200 0.200 2.600 0.800..
0.015 0.028 0.037 0.050 0.034 0.045..
0.030 0.056 0.075 0.100 0.068 0.090..
On selecting “SEND PLATE” option user can send the plate data to computer with the help of USB or SERIAL communication.
“LOAD NEXT” is used to load the next plate.
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Example Print-out obtained from ELISA READER
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(Note: Same Procedure can be used to create and run the test in all other modes, like
Single Standard, % Absorbance & Uptake)
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10. RERUNNING ACCEPTED TESTS / PROGRAMS
“ACCEPT TEST” option is used to store the data of controls or calibrators. After running any test first time with controls or calibrators, you select option “Accept Test” to save the data of controls or calibrators, so that next time when you want to load the same test, there is no need to load controls or calibrators in ELISA plate. You can use previously stored data.
A) In “CUT OFF” Mode:
Name: HCV
Pri: 450 Sec: 560
No Factor QC
BL 1 Y
NC 3 1.000 Y
PC 2 0.100 Y
LC 0 1.000 N
CC 0 1.000 N
POS- Cut Off
0.100 0.050
0.150 0.098
0.500 2.658
CUTFAC: 0.200
QC1 2.560
CUTABS: 0.563
QC2
QC3 QC4
ESC
QCVal Mes. Rem ACCEPT
TEST
Display
VALUE:
LOAD
NEXT
B) In “Multi standard” Mode:
Name: TSH
Pri: 450 Sec: 560
MULTI STANDARD
BL Y CAL 7
ESC
Range High > 16.61
HI CO : High >
LO CO : High >
Low < 10.30
Low < 0.000
NXT
Low < PREV
NO SAMP ABS AVG CONC REM PID
SAVE A1 B 0.050 0.050
B1 C1 0.070 0.070 0.000
C1 C2 0.257 0.257 2.500
---
RESULT
---
G1 S1 0.200 0.200 2.100 NEG TSH-1
H1 S2 1.854 1.854 9.845 POS TSH-2
LOAD PRINT PLATE
NXT MATRIX OPT.
VIEW
GRAPH
ACCEPT PRINT
TEST RESULTS
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When you want to run such an accepted test, select ‘RUN’ option in test detail screen, in “CUT OFF” mode:
Name: HCV
Pri: 450 Sec: 560
POS- Cut Off
ESC
No. of Samples : 0
DUP N SIMUL
RUN
PID
1 2 3 4 5 6 7 8 9 10 11 12
Load Blank?
Name: HBSAG
YES / NO
Pri: 450 Sec: 630
No. of Samples : 0
DUP N SIMUL
POS- Cut Off
1 2 3 4 5 6 7 8 9 10 11 12
Load Controls? YES / NO
ESC
RUN
PID
This time, the blank and controls will not be loaded directly and you will get the following questions: “Load Blank? YES / NO” and “Load Controls? YES / NO”.
If you are running both, select ‘YES’, and if you want to use earlier stored data of controls select ‘NO’, so that you can run only samples to get their results without loading Blank and Controls.
If you want to load only ‘Blank’, select “Load Blank? YES” and
“Load Controls? NO”.
In case if you are using previously Stored “Blank” and “Controls” then it will print following strings in the printout-
“Using Blank Stored value…..” and “Using Controls Stored value….”
R
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C) In Multi Standard / Single Standard / % Absorbance / Uptake Mode:
Name: TSH
Pri: 560 Sec: 0
No. of Samples: 0
MULTI STANDARD
ESC
RUN
DUP N SIMUL PID
1 2 3 4 5 6 7 8 9 10 11 12
Load Blank ? YES / NO
Name: TSH
Pri: 560 Sec: 0
MULTI STANDARD
No. of Samples: 0
ESC
RUN
DUP N SIMUL PID
1 2 3 4 5 6 7 8 9 10 11 12
Load Calibrators? YES / NO
In Calibrator Mode, after asking “Load Blank? Yes / No” you will get the following question “Load
Calibrators? Yes / No”. If you are loading new calibrators and want the results of samples as per new graph select “Load Calibrators? YES”.
Or else, If you are not loading new calibrators and want to use previously stored graph select “Load
Calibrators? No”.
If you want to load only ‘Blank’, select “Load Blank? YES” and “Load Calibrators? NO”.
In case if you are using previously Stored “Blank” and “Calibrators” then it will print following strings in the printout-
“Using Blank Stored value…..” and “Using Calibrator Stored value….”
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NOTE: User can view the Saved Graph from the Test parameter screen before running the same test.
Name: TSH MULTISTANDARD
Pri: 560 Sec: 0 BL Y 0.000 CAL 7 ESC
DUP Y DUP Y EDIT
RUN
HI CO: Y High> 25.03 Low< 10.54 PRINT
LO CO: Y High> 16.61 Low< 5.45
Interpret’n: Y Range High> 15.25
Low< 9.46
Graph: 4 PARAMETER
Y vs X : ABS vs CONC
To change values press EDIT
On selecting GRAPH option, it will display following screen where user can select “PLOT” option
in order to view the Valid GRAPH Screen.
No Conc Abs No Conc Abs
1 0.000 0.220
2 10.00 0.454
3 25.00 0.725
4 50.00 1.026
5 100.00 1.269
6 150.00 1.850
7 200.00 2.000
ESC PRINT PLOT
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The Graph screen will be displayed as shown below-
Origin at : X =0.000 , y = 0.000
X / div = 2.500 , y / div = 0.150
3.000
+
2.000
+
1.000
+
+
+
ESC
EDIT
0.001
0.000 10.00 20.00
+ +
40.00
11.SAMPLE AND SAMPLE DUPLICATE
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While loading the samples you can load a single sample in single well or a single sample in adjacent two well and finally the instrument will take the average of it as known as Sample
Duplicate.
The instrument will load the controls / calibrators automatically in any new test or not accepted test. You have to provide the number of samples. If you are loading single samples keep “DUP”-
No. Select “No. of Samples:” to enter total samples.
While loading the samples you can load a single sample in single well or a single sample in adjacent two well and finally the instrument will take the average of it known as Sample Duplicate.
The instrument will load the controls / calibrators automatically in any new test or not accepted test. You have to provide the number of samples. If you are loading single samples keep “DUP”-
No. Select “No. of Samples:” to enter total samples.
Version 150511
Name: HCV POS- Cut Off
Pri: 450 Sec: 560
DUP N
ESC
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NC
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PC
PC
Name: HCV
Pri: 450 Sec: 560
POS- Cut Off
ESC
No. of Samples: 0 RUN
DUP N SIMUL PID
1 2 3 4 5 6 7 8 9 10 11
12
Name: HCV
Pri: 450 Sec: 560
No. of Samples : 10
POS- Cut Off
ESC
RUN
DUP N SIMUL PID
1 2 3 4 5 6 7 8 9 10 11 12
B S3
NC S4
NC S5
NC S6
PC S7
PC S8
S1 S9
S2 S10
If you want to load duplicate samples select “DUP” option and then enter the number of samples by selecting “No. of Samples”
Name: HCV
Pri: 450 Sec: 560
POS- Cut Off
No. of Samples : 0
DUP Y SIMUL PID
ESC
RUN
1 2 3 4 5 6 7 8 9 10 11 12
B
NC
NC
NC
PC
PC
Name: HCV
Pri: 450 Sec: 560
No. of Samples : 0
DUP Y SIMUL
POS- Cut Off
PID
ESC
RUN
1 2 3 4 5 6 7 8 9 10 11 12
B
NC
NC
NC
PC
PC
Numeric Value: 10
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
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Name: HCV
Pri: 450 Sec: 560
POS- Cut Off
ESC
No. of Samples : 10
DUP Y SIMUL PID
RUN
1 2 3 4 5 6 7 8 9 10 11 12
B S2 S6 S10
NC S2 S6 S10
NC S3 S7
NC S3 S7
PC S4 S8
PC S4 S8
S1 S5 S9
S1 S5 S9
(* Similarly, user can load the samples and Sample Duplicates in all other modes)
12.PATIENT IDENTITY / SAMPLE IDENTITY ENTRY
A.) To enter a patient ID for all samples there is option provided in loading screen shown
as “PID”.
After entering number of samples either single or duplicate, select “PID”.
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A) Individual PID entry -
Name: HCV
Pri: 450 Sec: 560
No. of Samples: 10
DUP N SIMUL
POS- Cut Off
PID
ESC
RUN
1 2 3 4 5 6 7 8 9 10 11 12
B S3
NC S4
NC S5
NC S6
PC S7
PC S8
S1 S9
S2 S10
Sample
1
PID
PRST123
ESC
2
3
4
5
6
7
8
NEXT
9 PREV
10 SEQL
Sample PID
1 PRST123
ESC
2
3
4
5
6
7
8
NEXT
9 PREV
10 SEQL
PRST123
A B C D E F G H
I J K L M N O P ENTER
Q R S T U V W X CLEAR
Y Z , - + _ 1 2
3 4 5 6 7 8 9 0
One by one you can enter name of each patient. The character length of PID is only 7 characters in the following combinations –
Since PID of the Patient is entered with the help of Alpha-numeric characters, the combination must be as mentioned below.
Alphabets
4
3
2
Numbers
3
4
5
For example -
1. In the first combination, user can enter PID with 4 Alphabets and 3 Numbers. That means, entered PID should be PQRS123
2. Similarly, for the second combination, user can enter PID with 3 Alphabets and 4 Numbers.
And therefore, entered PID will be UTV1234
3. And for the third combination, user can enter PID with 2 Alphabets and 5 Numbers ie. PID will be displayed as RB12345
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4. But one can’t enter the PID with 1 Alphabet and 6 Numbers since its maximum numeric count is 65500.
(Note: The same combinations should be used during SEQUENTIAL entry also.)
B) Sequential Patient ID Entry
This one by one entry will take more time, therefore one more option to enter Patient Ids is provided. This option is “SEQL” sequential entry.
If you select “PID” option for patient ID entry, the following question appears on the screen:
“Delete Previous PIDs? Yes / No”.
If you select NO, it will directly load the previously entered
PIDs for sample and if you select YES, it will delete the previous PIDs.
Now select ‘SEQL’, it will display Alphanumeric screen where user can enter different sequential
PIDs. Follow the steps below-
Enter Alphabets
PRST
A B C D E F G H
I J K L M N O P ENTER
Q R S T U V W X CLEAR
Y Z , - + _ 1 2
3 4 5 6 7 8 9 0
Enter Initial Sample number
Numeric Value: 01
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR well location of any particular batch.
S
S
Enter last sample number
Numeric Value: 10
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
Enter Initial PID Number. This is the starting
Enter initial PID number
Numeric Value: 123
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
Enter last Well Number. This is the last well
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The final screen will appear which displays the sequential IDs of patient samples for the well location that is selected.
Sample
1
2
3
4
5
6
7
8
9
10
PID
PRST123
ESC
PRST124
PRST125
PRST126
PRST127
PRST128 NEXT
PRST129
PRST130 PREV
PRST131
PRST132 SEQL
Go to the next and previous screen with the help of “NEXT” and “PREV” option present on the screen.
(*NOTE: The entered PID get stored in instrument memory, until you delete it by using option,
“Delete previous PIDs? Yes / NO”)
13.SAVE PLATE AND SAVE RESULTS
In all modes of operation, there are two options provided to store results.
A) In cut Off mode:
Name: HCV POS- Cut Off
Pri: 450 Sec: 560 BL Y
Range PERCENTAGE % 10.00
ESC
NXT
NO SAMP ABS AVG CONC REM PID
A1 B 0.050 0.050
B1 NC
--
--
--
--
--
--
RESULTS
SAVE
RESULTS
MATRIX
PLATE
OPT.
PREV
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B) In Multi standard or any calibrator mode:
Name: TSH
Pri: 450 Sec: 0
Range High > 16.61
HI CO : High >
LO CO : High >
MULTI STANDARD
BL Y CAL 7
Low < 10.30
Low <
Low <
NO SAMP ABS AVG CONC REM PID
A1 B 0.050 0.050
B1 C1 0.070 0.070 0.000
C1 C2 0.257 0.257 2.500
--
--
--
--
LOAD PRINT PLATE
NXT MATRIX OPT.
VIEW
GRAPH:
ACCEPT
TEST
ESC
NXT
PREV
SAVE
RESULT
RESULTS
If “PLATE OPT.”is selected , you will obtain two options.
1. SAVE PLATE DATA ESC
2. SEND PLATE DATA
1. “SAVE PLATE DATA”, it helps the user to save the entire plate information along with the control/calibrator information (maximum 10 plates data can be saved).
2. “SEND PLATE DATA” is used to send the data from instrument to computer through USB or serial RS232 connection.
If “SAVE RESULT” option is selected the information of samples only except controls/calibrators, with test name, patient ID and date will be saved.
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The stored plate or stored results appear on the screen by selecting option “View Plate” in “MAIN
MENU”.
MAIN MENU
1 Test Operations ESC
1 PLATE RESULTS ESC
2 Technical Diagnosis
2 PATIENT RESULTS
3 Utilities
4 View Plate
5 Serial Communication
14.1. PLATE RESULT:
It displays the details of stored plate (last 10 plates) with date, test name and
mode of operation in descending order. User can select any test to view its plate
results by selecting this option.
1. PLATE RESULTS
2 PATIENT RESULTS
ESC
SIN_405 02/01/12
NO SAMP ABS AVG RES REM PID
A1 C1 0.083 0.083 50.00
B1 S1 0.544 0.544 326.7 POS VSP-1
C1 S2 0.367 0.367 220.3 NEG VSP-2
D1 S3 0.538 0.538 322.9 POS VSP-3
NO DATE TEST MODE ESC
4 02/01/12 SIN_405 S
3 01/01/12 TSH M SEL
E1 S4 0.300 0.300 180.2 NEG VSP-4
F1 S5 0.700 0.700 420.0 POS VSP-5
2 30/12/11 HBSAG C
1 28/12/11 CPC C
4 02/01/12 SIN_405 S
Press Select key ot proceed
-- -- --- --- ---- --- ---
-- -- --- --- ---- --- ---
G2 S14 0.326 0.326 195.6 NEG VSP-14
H2 S15 0.491 0.491 294.7 EQ VSP-15
ESC NEXT PREV PRINTMATRIX SENDDATA PRINT
“Print Matrix” will print the details of entire plate in matrix format as explained earlier.
“Print” will print only the data displayed on screen.
“Send data” can be used to send the data from instrument to computer through USB or serial
RS232 connection.
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Note: User can directly view the details of Plate Results by accessing it through Plate Data option present on the Select Test screen.
Select Test ESC
1. HBSAg
2. HCVAb M RUN / SEL
3. UPT
4. TSH
5. CPC C
6. PER_ABS P NEXT
7. CRP C
8. KIN_405 K PREV
9. SIN_630 S
10. CP_560 C DEL
PLATE
DATA
14.3. PATIENT RESULTS
It will show the details of all samples, saved in memory using option “SAVE RESULTS”
in all different modes, as explain earlier. This option will display the data in table format
as shown below. Test name, Mode of test operation, result of sample, PID and Remark will
appear on the screen. The instrument has a memory to store 2500 sample results.
This option doesn’t show the information of controls or calibrators.
01 / 01 / 08
Test M Result PID Remarks
1 PLATE RESULTS ESC
2 PATIENT RESULTS
MUL_4P M 10.48 VSR1 EQ
MUL_4P M 12.63 VSR2 POS
MUL_4P M 7.450 VSR3 NEG
MUL_4P M 13.80 VSR4 POS
SIN405 S 267.7 NRA1 EQ
SIN405 S 220.3 NRA2 NEG
SIN405 S 180.2 NRA3 NEG
SIN405 S 326.7 NRA4 POS
ESC NEXT PREV PRINT
“PRINT” is used to print the details as available on the screen.
“NEXT” and “PREV” options are used to browse through the pages so that one can view
all the results one after the other.
NOTE:
1. When there is not enough memory the following message appears:
“Memory Left for samples- First Samples Deleted”
The instrument automatically deletes a number of samples that are stored in the beginning
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and then save the new samples at that location.
2. If a test is being deleted or edited then previously stored results of that particular test are displayed with their test mode as "X". But test name retained as it is.
01 / 01 / 08
Test M Result PID Remarks
MUL_4P X 10.48 VSR1 EQ
MUL_4P X 12.63 VSR2 POS
MUL_4P X 7.450 VSR3 NEG
MUL_4P X 13.80 VSR4 POS
SIN405 S 267.7 NRA1 EQ
SIN405 S 220.3 NRA2 NEG
SIN405 S 180.2 NRA3 NEG
SIN405 S 326.7 NRA4 POS
ESC NEXT PREV PRINT
15. UTILITIES
UTILITIES consists of 11 different functions which can be viewed as follows-
MAIN MENU
1 Test Operations
2 Technical Diagnosis
3 Utilities
4 View Plate
5 Serial Communication
ESC
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : USB
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
10 Password
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15.1. Date & Time Setting
This Utility helps the user to set current Date and Time.
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : USB
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
10 Password
Date – DD / MM / YY
01 / 01 / 08
Time – Hrs : Min : Sec
12 : 30 : 10
15.2. Program Filter
It consists of two editable filters and thus helps the user to edit the filters if any.
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : USB
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
10 Password
Edit Filters? YES / NO
XXX
Numeric Value : 250
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
XXX 250
YYY
Numeric Value : 400
1 2 3 4 5 6 ENTER
7 8 9 0 . CLEAR
ESC
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15.3. Printer On / Off setting:
Its a toggle between Printer ON and Printer OFF
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : USB
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
10 Password
15.4. Enter Clinic Name:
Type your Clinic Name and select “ENTER”. The character length is maximum 19 characters.
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : USB
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
10 Password
UTILITIES Version (3.400A)
CLINIC
TATA_MEMORIAL
A B C D E F G H
I J K L M N O P ENTER
Q R S T U V W X CLR
Y Z , - + _ 1 2
3 4 5 6 7 8 9 0
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15.5. Shaker (Plate Shaking Mode)
Shaker is used for shaking the plate before running any test. The Shaker screen is displayed as follows -
ESC Shake Time 0 sec
Shake Speed 0
Start
Stop
Shake Time 0 sec
Shake Speed 0
Start
ESC
Stop
Numeric Value: 20
1 2 3 4 5 6 ENTR
7 8 9 0 . CLR
Shake Time 20 sec
Shake Speed 0
Start
Stop
Numeric Value: 05
1 2 3 4 5 6 ENTR
7 8 9 0 . CLR
ESC
The total shake time can be entered in seconds
Regarding the “Shake Speed”, there are 10 types of speeds. You can choose any speed by entering numbers from 1 to 10. The shaking speed increases as the number increases.
After entering shake time and shake speed, select Start option. When “Start” is selected, the tray will come out and on the screen you will obtain a message “Is plate loaded? Yes / No”. Load the plate and select YES option.
Shake Time 20 sec
Shake Speed 5
Start
Stop
ESC
Shake Time 20 sec
Shake Speed 5
Start
Stop
Is Plate Loaded? Yes / NO
ESC
After selecting ‘YES’ the tray will go inside and instrument will do the shaking of plate for the given time period with selected speed.
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When the shaking is finished, the plate holder will come out and display a message-
“Is plate removed? Yes / No”. Remove the plate and select ‘YES’ to end the process.
Shake Time 20 sec
Shake Speed 5
Start
Stop
ESC
Is Plate Removed ? Yes / NO
Similarly, to stop the Shaking process in between select “STOP” option present on the screen.
After selecting STOP option it will display the message string – ‘Is Plate Removed? YES / NO’.
Select NO to terminate the ongoing process.
Note: ‘HOME’ key is also used for directly accessing the shaking mode (ie. SHAKER)
15.6. Communication Settings:
It is a toggle between USB and SERIAL communication. User can either switch to USB or Serial communication by connecting their respective cables.
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : USB
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
10 Password
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : SERIAL
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
10 Password
This setting is very important, whenever you want to transfer data from instrument to computer.
You can transfer data either using USB or by Serial RS232 cable.
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15.8. Install USB Driver:
This option present in the Utilities helps the user to install the USB Drivers in their respective computers. Remember that before installing the USB drivers you must first switch on to the
USB communication settings.
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : USB
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
10 Password
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : USB
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
Install USB Drivers ? YES / NO
15.9. Auto Print: ON
It is a toggle key between Auto Print: ON and Auto Print: OFF.
After completing the running process it automatically takes the print of the result and its
graph if any in order to maintain the record of the plate run in the form of hard copy.
Auto Print is active in all modes excluding ABS mode. For Auto Print, user must set the printer ON otherwise it will display the error string “Disable printer YES / NO?”
NOTE: The option 8. “CV Diagnostic OFF” is not for users. It is for factory use only.
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15.10. PASSWORD:
Select Password option in order to Set the Password so that one can’t ADD, EDIT,
DELETE and SEND / RECEIVE Test records without entering the valid Password.
UTILITIES Version (3.400A)
1 Date and Time ESC
2 Program Filter
3 Printer On / Off: ON
4 Enter Clinic Name
5 Shaker
6 Communication Settings : USB
7 CV Diagnostic OFF
8 Install USB Driver
9 Auto Print: OFF
10 Password
Password Utility
1 Enable / Disable ESC
2 Set Password
3 LOGIN
4 LOGOUT
15.10.1. ENABLE / DISABLE:
Enable / Disable helps the user to set the Password or Remove the Password from the
Password Utility. Enter Password with the help Alphanumeric screen which is displayed after selecting Enable / Disable option.
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Password Utility
1 Enable / Disable ESC
2 Set Password
3 LOGIN
4 LOGOUT
ESC
ADMIN PASSWORD
A B C D E F G H
I J K L M N O P ENTER
Q R S T U V W X CLR
Y Z , - + _ 1 2
3 4 5 6 7 8 9 0
15.10.2. SET PASSWORD:
This option helps to set the PASSWORD along with the USER name.
Password Utility
1 Enable / Disable ESC
2 Set Password
3 LOGIN
4 LOGOUT
USER TANIYA
A B C D E F G H
ADMIN PASSWORD
A B C D E F G H
I J K L M N O P ENTER
Q R S T U V W X CLR
Y Z , - + _ 1 2
3 4 5 6 7 8 9 0
Enter “PASSWORD” and select ENTER
I J K L M N O P ENTER
Q R S T U V W X CLR
Y Z , - + _ 1 2
3 4 5 6 7 8 9 0
Enter appropriate USER name and select ENTER
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15.10.3. LOGIN:
After entering the Password user needs to select LOGIN.
Password Utility
1 Enable / Disable ESC
2 Set Password
3 LOGIN
4 LOGOUT
USER TANIYA
A B C D E F G H
I J K L M N O P ENTER
Q R S T U V W X CLR
Y Z , - + _ 1 2
3 4 5 6 7 8 9 0
To Login/ enable user, enter the USER name and select ENTER
15.10.4. LOGOUT:
Password Utility
1 Enable / Disable ESC
2 Set Password
3 LOGIN
4 LOGOUT
Password Utility
1 Enable / Disable ESC
2 Set Password
3 LOGIN
4 LOGOUT
Logout
To Logout / disable user, select LOGOUT option
present on the screen
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16. SERIAL COMMUNICATION
The instrument is equipped with an RS232 serial port for PC configuration ie. User-computer interface. A cable (a NULL-MODEM cable) is required (not provided) to link the instrument to PC.
Communication will only start when both ends detect the presence of an active terminal or device.
RS232 port settings in a windows Operating system.
PORT SETTINGS
Bits per second : 9600
Data Bits : 8
Parity None : None
Stop Bits : 1
Flow control : None
There is an option present on the Main menu screen named as SERIAL Communication which is used for transferring data from instrument to the computer and also from instrument to instrument.
MAIN MENU ESC
1. Test Operations
2. Technical Diagnosis
3. Utilities
4. View Plate
5. Serial communications
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16.1. Send Results:
SEND RESULTS helps the user to transfer all the Results stored in the instrument to the computer via Serial Communication.
Results can be send either through USB or SERIAL communication.
Before Sending the Results :
- A driver and the corresponding Data Transfer application must be downloaded on your computer.
Further information can be requested to our distributor or to our technical support Department,
-check whether the Serial cable / USB cable is connected to both the ends of the instrument and the computer respectively.
MAIN MENU
1. Test Operations ESC
2. Technical Diagnosis
SERIAL COMM.
ESC
1 Send Results
2 Send Test Records
3 Receive Test Records
3. Utilities
4. View Plate
5. Serial communications
SERIAL COMM. ESC
1 Send Results
2 Send Test Records
3 Receive Test Records
Sending…..
SERIAL COMM. ESC
1 Send Results
2 Send Test Records
3 Receive Test Records
Send Results? YES / NO
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These Results are send through Application, and the Excel file is created as shown below –
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16.2. Send Test Records:
This option helps the user to send the Test Records created in one instrument to another instrument serially with the help of serial cable. And therefore one has to select serial communication in Utilities.
SERIAL COMM.
ESC
1 Send Results
2 Send Test Records
3 Receive Test Records
SERIAL COMM.
ESC
1 Send Results
2 Send Test Records
3 Receive Test Records
Send Test Records? YES / NO
Suppose by default if Serial Communication is set to USB then it will display following screen with msg string.
SERIAL COMM.
1 Send Results
2 Send Test Records
3 Receive Test Records
Invalid in USB communication !!!
ESC
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16.3. Receive Test Records:
This option is used for receiving the Test Records from one instrument to another. On selecting “Receive Test Records”; it will display the string – Download Test Records? Yes /
No. Select “Yes” in order to receive the data from the other instrument.
ESC
1 Send Results
2 Send Test Records
3 Receive Test Records
SERIAL COMM. SERIAL COMM.
ESC
1 Send Results
2 Send Test Records
3 Receive Test Records
Download Test Records ? YES / NO
Note: 1. User must first restart the instrument before sending the data from one instrument to
another.
2. Remember that while transferring Test Records from one instrument to another
one must first select “Receive Test Records” and then select
“Send Test Records” in order to send the data from one instrument to another.
For instance, let us take an example which will show how the Test Records are
transferred from instrument to instrument.
Let “Instrument 1” resemble the screen of the Instrument which will receive the Test
Records from other instrument. And “Instrument 2” resemble the screen of the
Instrument which will send the Test Records to other instrument.
Instrument 1
MAIN MENU ESC
SERIAL COMM.
1. Test Operations
1 Send Results
ESC
2. Technical Diagnosis
3. Utilities
4. View Plate
5. Serial communications
2 Send Test Records
3 Receive Test Records
Download Test Records ? YES / NO
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Now for Sending Test Records switch on to Instrument 2.
Instrument 2
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MAIN MENU ESC
1. Test Operations
2. Technical Diagnosis
3. Utilities
4. View Plate
5. Serial communications
SERIAL COMM.
1 Send Results
2 Send Test Records
3 Receive Test Records
ESC
Send Test Records? YES / NO
Select “YES” in order to send all the test records
SERIAL COMM.
1 Send Results
2 Send Test Records
3 Receive Test Records
Sending……..
ESC
And similarly, Instrument 1 will display the following screen which denotes that the instrument is downloading the Test Records.
SERIAL COMM.
1 Send Results
2 Send Test Records
3 Receive Test Records
1248
ESC
After completing the process of transferring the data, one must restart both the instrument and check for the data transferred between the instruments
In this way, one can transfer Test Records from instrument to instrument.
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3. User can Send and Receive the Test Records only through serial cable and
therefore one has to set the Serial Communication: SERIAL in Utilities.
If Serial Communication is set to USB, then it will display following screen –
SERIAL COMM.
1 Send Results
2 Send Test Records
3 Receive Test Records
Invalid in USB communication !!!
ESC
17. TECHNICAL DIAGNOSIS
Technical Diagnosis is present in “Main Menu”.
MAIN MENU
1 Test Operations
2 Technical Diagnosis
3 Utilities
4 View Plate
5 Serial Communication
ESC
TECHNICAL DIAGNOSIS
1. Clear Memory
2. Lamp Amplitude
3. Printer Self test
4. Lamp Centering Check
ESC
17.1. Clear Memory
This option is used to clear entire saved test records and entire saved patient results along with saved plates. The “Test Records” are the different kind of programmed test that are made in different modes of operation and saved in instrument program memory.
TECHNICAL DIAGNOSIS ESC
1. Clear Memory
2. Lamp Amplitude
3. Printer Selftest
Clear Results? YES / NO
Version 150511 PI number: 1701327
TECHNICAL DIAGNOSIS
1. Clear Memory
2. Lamp Amplitude
3. Printer Selftest
ESC
Clear Test Records? YES / NO
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When option “Clear Memory” is selected the instrument will first ask “Clear Results YES / NO”.
If “YES”is selected it will only clear saved patient results and saved plates stored in the memory, and shows message “Clearing Memory …”.
This option does not clear the programmed test saved in instrument memory.
After clearing the memory or if option “NO” is selected the next question appears.
17.2. Lamp Amplitude
This option is used to check the light gain of filters used in the instrument. There are 8- channels used of same type. This option shows the gain of each channel filter in table format. In the instrument the range of amplitude is from 600.00 to 2450.00. If the amplitude goes below 600.00 for any channel, the message “Amp –Low” will appear on the screen for that particular channel if the amplitude goes more than 2490.00, than the over range saturation voltage figure of 2500.00 will be passed.
The gains of filters are set within a required range. When you select this option, the lamps will turn
ON, tray holder plate will comes out and the instrument will start showing amplitudes (gains) of all filters one by one.
TECHNICAL DIAGNOSIS
1. Clear Memory
2. Lamp Amplitude
3. Printer Self test
4. Lamp Centering Check
ESC
LAMP AMPLITUDE
1 Stability Check
2 Filter Amplitude
ESC
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1. Stability Check:
LAMP AMPLITUDE
1 Stability Check
2 Filter Amplitude
ESC
LAMP AMPLITUDE
1 Stability Check
2 Filter Amplitude
ESC
Displays the Lamp amplitude of a single selected filter.
Filter : 405 ESC
CH1 CH2 CH3 CH4
1921.8 2011.7 1983.7 1875.2
1920.4 2010.3 1982.4 1874.2
1919.4 2009.3 1981.7 1873.4
1918.5 2008.5 1981.2 1872.9
1918.0 2007.8 1980.8 1872.5
--- ---- --- ----
--- ---- --- ----
--- ---- ---- ---
CH5 CH6 CH7 CH8
1921.0 1868.4 1899.1 1848.0
1919.9 1867.3 1898.1 1846.6
1919.2 1866.5 1897.4 1845.8
1918.7 1866.0 1896.9 1844.9
---- ---- ---- ----
--- ---- ---- ----
LAMP AMPLITUDE
1 Stability Check
2 Filter Amplitude
Select Filter Val:
0 405 450
492 630 NA
NA 560 YYY
ESC
Filter : 560 ESC
CH1 CH2 CH3 CH4
1997.6 1869.1 2042.2 2014.2
1997.5 1868.9 2042.3 2014.3
1997.2 1868.9 2042.3 2014.5
1997.1 1868.9 2042.5 2014.6
1997.0 1868.7 2042.6 2014.7
--- ---- --- ----
--- ---- --- ----
--- ---- ---- ---
CH5 CH6 CH7 CH8
1904.8 2007.7 1891.1 1935.2
1904.7 2007.7 1891.1 1934.9
1904.9 2007.7 1891.2 1935.0
1905.1 2007.9 1891.3 1934.9
---- ---- ---- ----
--- ---- ---- ----
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17.3. Printer Self test
It is used to check the printing of thermal printer. Is it printing perfectly or not?
When you select this, it will print first two lines of Technical Diagnosis Screen.
17.4. Lamp Centering Check:
NOTE: “Lamp Centering Check” is not for users. Do not select this option and
don’t change any settings otherwise the mechanical alignment will get
disturbed, since it is for factory use only.
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18. Trouble Shooting
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1) Printer disabled
Disable Printer YES / NO?
2) “Check Light Path!!! CH x”
Where ‘x’ is any channel number
X = 1/2/3/4/5/6/7/8
CAUSE /CORRECTIVE ACTION
You will get this message, if paper is not loaded properly or lever is not at correct position. Check all possibilities.
Also check ON LINE and FEED LED glowing or not and when you switch ‘ON’ the instrument check paper is moving forward or not. If LEDs are not glowing and paper is not moving forward contact service engineer.
You can get this message in any mode of operation. Before reading absorbance, instrument is checking the filter reference voltages of all 8 channels. If filter voltage of any channel is less than minimum required voltage, it will display a message “Check Light Path!!! CH x”. Here ‘x’ is channel numbers having reference voltage less than minimum required.
For ex. If reference voltage of channel 3 is less the message will be displayed “Check Light Path!!! CH 3”. It will indicate all channel numbers having less reference voltage, like
“Check Light Path!!! CH 1 3 6 ”
In such a case, there is a possibility of filter gain of that channel is reduced or intensity of lamp of that channel has become poor, so contact factory engineer.
3) Invalid Assay
If in the Cut Off and Multi standard mode the controls and standards are not ok then this error message will appear.
4) Memory Full
If Number of saved tests exceeds the memory limit of 250 tests then delete the unwanted tests and save the test.
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Using data receiving application on computer,
1) If you have not connected the data cable (USB or Serial) correctly, you will get this message.
2) If you have selected ‘Communication Setting’ (ref.
14.5), USB on instrument, and you have selected a ‘Serial’
5) “Check USB Application on communication on computer data receiving application,
Computer you will get this message. In such a case select ‘USB’
Do you want to continue? communication, on computer and select ‘YES’ key to
YES / NO” transfer data.
To avoid this message, connect proper data cable, select the same communication medium (USB or Serial), on computer data receiving application, which you have selected on instrument ‘Communication Setting’.
6) Filter Movement Error!!!
7) Plate Movement Error!!!
Instrument will display this message, if there is problem with MOC sensor PCB or with filter tray stepper motor.
The MOC sensors are used to detect the position of filter tray. These two sensors are mounted on bottom of the mechanism. One detects the home position and second detects the each filter position of filter tray. In such a condition we need to check the connection to these sensor and also the connection of stepper motor, which is used to move filter tray.
Instrument will display this message, if there is problem with MOC sensor PCB or with plate carrier stepper motor.
The MOC sensors are used to detect the position of plate carrier. These two sensors are mounted on top of the mechanism. One detects the home position and second detects the each strip position of plate carrier. In such a condition we need to check the connection to these sensor and also the connection of stepper motor, which is used to move plate carrier.
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19. DECONTAMINATION
19.1. Decontamination Procedure
If the instrument is to be shipped after being exposed to potentially hazardous material, it should be decontaminated. The following procedure outlines the method of decontaminating the instrument before packaging and shipment.
19.2. Purpose of Decontamination
Decontamination minimizes the risk to all who come in contact with the instrument during shipping, handling, and servicing.
19.3. General Considerations
Any laboratory instrument that has been used for clinical analysis is considered a biohazard and should be decontaminated prior to handling. Intact skin is generally considered an effective barrier against infectious Organisms; however, small abrasions and cuts may not be always being visible. Prophylactic gloves must be worn when handling instruments that have not been decontaminated. Gloved hands should be considered contaminated at all times and must be kept away from eyes, mouth and nose at all times.
Mucous membranes are considered prime entry routes for infectious agents. Wear eye protection and a surgical mask when there is a possibility of aerosols.
Eating and drinking while decontaminating instruments is not advisable.
19.4. Procedure
A solution of .5% Sodium Hypo Chlorite (NaOCL) solution (Bleach) is used.
Commercial bleach is 5% NaOCL; household bleach is 3% NaOCL. When using commercial bleach, use a 10:1 mixture; if using household bleach, a 6:1 mixture is required. This is a caustic solution. It is important to wear gloves and eye protection when handling it.
Wipe down the carrier and all exposed surfaces of the unit with the bleach solution.
Remove the top shroud of the instrument and wipe down the top surface of the instrument base, as well as the inside of the top shroud.
Rinse all the decontaminated carrier and exposed surfaces will distilled water.
Reassemble the unit and discard the used gloves and towels.
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20. SAFETY CLEARANCE CERTIFICATE
Please complete all information requests on this form prior to returning the instrument to the manufacturer or your local distributor for servicing, repairs or return. Thank you for your co-operation.
Customer Contact
Address
Dept
Tel:
Position
Country
Post Code
Model No.
Accessories
Returned
Fax:
Serial No.
Date of Purchase (if known)
Complaint
Has the equipment been exposed to any of the following: (*delete as applicable) a) Blood, body fluids, pathological specimens *YES/NO
If YES, please specify
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If YES, Please specify
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*YES/NO
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