CY-8063 S100A11 ELISA Kit

S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
ELISA Kit for Measuring Human S100A11
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CircuLex S100A11 ELISA Kit
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Intended Use................................................ 1
Storage......................................................... 1
Introduction.................................................. 2
Principle of the Assay.................................. 2-3
Materials Provided....................................... 3
Materials Required but not Provided........... 4
Precautions and Recommendations.............. 5
Sample Collection and Storage......................6
Detailed Protocol......................................... 7-8
Calculations.............................…................. 9
Measurement Range..................................... 9
Troubleshooting............................................ 10
Reagent Stability......................................... 10
Assay Characteristics.................................. 11-13
Example of Test Results...............................14-15
References.................................................... 16
Related Products.......................................... 16-17
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Cat# CY-8063
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Intended Use
The CycLex Research Product CircuLex S100A11 ELISA Kit is used for the quantitative
measurement of human S100A11 in serum, plasma, cell lysate and other biological media.
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This assay kit is for research use only and not for use in diagnostic or therapeutic procedures.
Storage
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• Upon receipt store all components at 4°C.
• Don’t expose reagents to excessive light.
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Cat#: CY-8063
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
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Introduction
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S100A11 is a member of the S100 family of EF-hand Ca2+-binding proteins, whose expression is
ubiquitous in various tissues including skin, smooth muscle and other tissues at different levels, with a
high expression level in the skin. It is also known as calgizzarin (1) and S100C (2). S100A11 was shown
to bind to annexin A1 and the S100A11/annexin A1 complex is a heterotetramer consisting of two
S100A11 and two annexin A1 proteins. Ca2+ binding to S100A11 induces a conformational change that
exposes a hydrophobic surface for interaction with target proteins (3). In addition to binding to annexin
A1, S100A11 has been shown to interact with annexin A6 (4), actin (5) and transglutaminase (6), and is
capable of forming a heterodimer with S100B through subunit exchange (7).
It was reported that S100A11, which actively secreted by NHK (normal human keratinocyte), acts on
NHK to enhance the production of EGF family proteins, resulting in growth stimulation (8). On the
other hand, inhibitory signals such as TGFβ or high-calcium promote S100A11/annexin A1 to
translocate into the nucleus and induce p21/WAF1 resulting in growth inhibition (9). These findings
indicate that S100A11 plays a dual role in growth regulation, being suppressive in cells and being
promotive from outside of cells. S100A11 seems to act as either a tumor suppressor or promoter in many
different types of tumors and would play respective roles in influencing the proliferation of the cancer
cells.
Principle of the Assay
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The CycLex Research Product CircuLex S100A11 ELISA Kit employs the quantitative sandwich
enzyme immunoassay technique. A mouse monoclonal antibody specific for human S100A11 is
pre-coated onto a microplate. Standards and samples are pipetted into the wells and the immobilized
antibody binds any human S100A11 present. After washing away any unbound substances, an HRP
conjugated antibody specific for human S100A11 is added to the wells. Following a wash to remove any
unbound antibody HRP conjugate, the remaining conjugate is allowed to react with the substrate
H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the
resulting yellow product is measured at 450 nm. The absorbance is proportional to the concentration of
human S100A11. A standard curve is constructed by plotting absorbance values versus human S100A11
concentrations of calibrators, and concentrations of unknown samples are determined using this standard
curve.
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
Summary of Procedure
Add 100 µL of diluted samples to the wells
Incubate for 1 hour at room temp.
Wash the wells
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Add 100 µL of HRP conjugated anti-human S100A11 antibody
Wash the wells
Add 100 µL of Substrate Reagent
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Add 100 µL of Stop Solution
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Incubate for 1 hour at room temp.
Measure absorbance at 450 nm
Materials Provided
All samples and standards should be assayed in duplicate. The following components are supplied and
are sufficient for the one 96-well microplate kit.
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Microplate: One microplate supplied ready to use, with 96 wells (12 strips of 8-wells) in a foil, zip-lock
bag with a desiccant pack. Wells are coated with mouse anti-human S100A11 monoclonal antibody
(YK-1A4) as a capture antibody.
10X Wash Buffer: One bottle containing 100 mL of 10X buffer containing 2%Tween®-20
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Dilution Buffer: Two bottles containing 20 mL of 1X buffer; use for sample dilution. Ready to use.
Human S100A11 Standard: One vial containing 4 ng of lyophilized recombinant human S100A11
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HRP conjugated Detection Antibody: One bottle containing 12 mL of HRP (horseradish peroxidase)
conjugated anti-human S100A11 antibody. Ready to use.
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Substrate Reagent: One bottle containing 20 mL of the chromogenic substrate, tetra-methylbenzidine
(TMB). Ready to use.
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Stop Solution: One bottle containing 20 mL of 1 N H2SO4. Ready to use.
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S100A11 ELISA Kit
User’s Manual
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Materials Required but not Provided
• Pipettors: 2-20 µL, 20-200 µL and 200-1000 µL precision pipettors with disposable tips
• Precision repeating pipettor
• Orbital microplate shaker
• Microcentrifuge and tubes for sample preparation
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• Vortex mixer
• Microplate washer: optional (Manual washing is possible but not preferable)
• Plate reader: capable of measuring absorbance in 96-well plates at dual wavelengths of 450/540 nm.
Dual wavelengths of 450/550 or 450/595 nm can also be used. The plate can also be read at a single
wavelength of 450 nm, which will give a somewhat higher reading.
• 500 or 1000 mL graduated cylinder
• Reagent reservoirs
• Deionized water of the highest quality
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• Disposable paper towels
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• Software package facilitating data generation and analysis :optional
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Cat#: CY-8063
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
Precautions and Recommendations
• Wear gloves to avoid S100A11 contamination from your skin.
• Allow all the components to come to room temperature before use.
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• All microplate strips that are not immediately required should be returned to the zip-lock pouch, which
must be carefully resealed to avoid moisture absorption.
• Use only the microtiter wells provided with the kit.
• Rinse all detergent residues from glassware.
• Use deionized water of the highest quality.
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• Do not mix reagents from different kits.
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• Do not use kit components beyond the indicated kit expiration date.
• The buffers and reagents in this kit may contain preservatives or other chemicals. Care should be taken
to avoid direct contact with these reagents.
• Do not mouth pipette or ingest any of the reagents.
• Do not smoke, eat, or drink when performing the assay or in areas where samples or reagents are
handled.
• Dispose of tetra-methylbenzidine (TMB) containing solutions in compliance with local regulations.
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• Avoid contact with the acidic Stop Solution and Substrate Solution, which contains hydrogen peroxide.
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• Wear gloves and eye protection when handling immunodiagnostic materials and samples of human
origin, and these reagents. In case of contact with the Stop Solution and the Substrate Solution, wash
skin thoroughly with water and seek medical attention, when necessary.
• Biological samples may be contaminated with infectious agents. Do not ingest, expose to open
wounds or breathe aerosols. Wear protective gloves and dispose of biological samples properly.
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• CAUTION: Sulfuric Acid is a strong acid. Wear disposable gloves and eye protection when
handling Stop Solution.
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S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
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Sample Collection and Storage
Serum: Use a serum separator tube and allow samples to clot for 60 ± 30 minutes. Centrifuge the
samples at 4°C for 10 minutes at 1,000 x g. Remove serum and assay immediately or store samples on
ice for up to 6 hours before assaying. Aliquots of serum may also be stored at below -70°C for extended
periods of time. Avoid repeated freeze-thaw cycles.
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Plasma: Collect plasma using EDTA-Na2 as the anticoagulant. If possible, collect the plasma into a
mixture of EDTA-Na2 and Futhan5 to stabilize the sample against spontaneous in vitro complement
activation. Immediately centrifuge samples at 4°C for 15 minutes at 1,000 x g. Assay immediately or
store samples on ice for up to 6 hours before assaying. Aliquots of plasma may also be stored at below
-70°C for extended periods of time. Avoid repeated freeze-thaw cycles.
Note: Heparin and Citrate plasma has not been validated for use in this assay.
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Other biological samples: Remove any particulates by centrifugation and assay immediately or aliquot
and store samples at below -70°C. Avoid repeated freeze-thaw cycles.
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Cat#: CY-8063
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Version#: 140318
CircuLex
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
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Detailed Protocol
The CycLex Research Product CircuLex S100A11 ELISA Kit is provided with removable strips of
wells so the assay can be carried out on separate occasions using only the number of strips required for
the particular determination. Since experimental conditions may vary, an aliquot of the human S100A11
Standard within the kit, should be included in each assay as a calibrator. Disposable pipette tips and
reagent troughs should be used for all liquid transfers to avoid cross-contamination of reagents or
samples.
Preparation of Reagents
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All reagents need to be brought to room temperature prior to the assay. Assay reagents are supplied
ready-to-use, with the exception of 10X Wash Buffer and Human S100A11 Standard.
1. Prepare a working solution of Wash Buffer by adding 100 mL of the 10X Wash Buffer to 900 mL of
deionized (distilled) water (ddH2O). Mix well. Store at 4°C for two weeks or -20°C for long-term
storage.
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2. Reconstitute Human S100A11 Standard with 1.0 mL of ddH2O. The concentration of the S100A11
in vial should be 4 ng/mL, which is referred as a Master Standard of S100A11.
Prepare Standard Solutions as follows:
Use the Master Standard to produce a dilution series (below). Mix each tube thoroughly before
the next transfer. The 1,000 pg/mL standard (Std.1) serves as the highest standard. The Dilution
Buffer serves as the zero standard (Blank).
Volume of Standard
150 µL of Master Standard (4 ng/mL)
300 µL of Std. 1 (1,000 pg/mL)
300 µL of Std. 2 (500 pg/mL)
300 µL of Std. 3 (250 pg/mL)
300 µL of Std. 4 (125 pg/mL)
300 µL of Std. 5 (62.5 pg/mL)
300 µL of Std. 6 (31.3 pg/mL)
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Std.1
Std.2
Std.3
Std.4
Std.5
Std.6
Std.7
Blank
-
Dilution Buffer
450 µL
300 µL
300 µL
300 µL
300 µL
300 µL
300 µL
300 µL
Concentration
1,000 pg/mL
500 pg/mL
250 pg/mL
125 pg/mL
62.5 pg/mL
31.3 pg/mL
15.6 pg/mL
0 pg/mL
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Note: Do not use a Repeating pipette. Change tips for every dilution. Wet tip with Dilution Buffer
before dispensing. Unused portions of Master Standard should be aliquoted and stored at below
-70°C immediately. Avoid multiple freeze and thaw cycles.
Sample Preparation
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• Serum and plasma samples require a 10-20 fold dilution.
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• Other biological samples require neat to appropriate dilution. Optimal dilutions of cell conditioned
medium for measurement are indicated below;
A431 cell culture medium: 200-400 fold dilution
HeLa cell culture medium: 30-60 fold dilution
LoVo cell culture medium: 30-60 fold dilution
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Cat#: CY-8063
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
Standard Assay Procedure for Human S100A11
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1. Remove the appropriate number of microtiter wells from the foil pouch and place them into the well
holder. Return any unused wells to the foil pouch, refold, seal with tape and store at 4°C.
2. Dilute samples with Dilution Buffer. (See “Sample Preparation” above.)
3. Pipette 100 µL of Standard Solutions (Std1-Std7, Blank) and the diluted samples in duplicates,
into the appropriate wells.
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4. Incubate the wells at room temperature (ca.25°C) for 1 hour, shaking at ca. 300 rpm on an orbital
microplate shaker.
5. Wash 4-times by filling each well with Wash Buffer (350 µL) using a squirt bottle, multi-channel
pipette, manifold dispenser or microplate washer.
6. Add 100 µL of HRP conjugated Detection Antibody into each well.
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7. Incubate the wells at room temperature (ca.25°C) for 1 hour, shaking at ca. 300 rpm on an orbital
microplate shaker.
8. Wash 4-times by filling each well with Wash Buffer (350 µL) using a squirt bottle, multi-channel
pipette, manifold dispenser or microplate washer.
9. Add 100 µL of Substrate Reagent to each well. Avoid exposing the microtiter plate to direct
sunlight. Covering the wells with e.g. aluminum foil is recommended. Return Substrate Reagent to
4°C immediately after the necessary volume is removed.
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10. Incubate the wells at room temperature (ca.25°C) for 10-20 minutes, shaking at ca. 300 rpm on an
orbital microplate shaker. (The incubation time may be extended up to 30 minutes if the reaction
temperature is below than 20°C).
11. Add 100 µL of Stop Solution to each well in the same order as the previously added Substrate
Reagent.
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12. Measure absorbance in each well using a spectrophotometric microplate reader at dual wavelengths of
450/540 nm. Dual wavelengths of 450/550 or 450/595 nm can also be used. Read the microplate at
450 nm if only a single wavelength can be used. Wells must be read within 30 minutes of adding the
Stop Solution.
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Note-1: Complete removal of liquid at each step is essential to good performance. After the last wash,
remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it
against clean paper towels.
Note-2: Reliable standard curves are obtained when either O.D. values do not exceed 0.25 units for the
blank (zero concentration), or 3.0 units for the highest standard concentration. The plate
should be monitored at 5-minute intervals for approximately 30 minutes.
Note-3: If the microplate reader is not capable of reading absorbance greater than the absorbance of the
highest standard, perform a second reading at 405 nm. A new standard curve, constructed
using the values measured at 405 nm, is used to determine S100A11 concentration of
off-scale samples. The readings at 405 nm should not replace the on-scale readings at 450 nm.
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
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Calculations
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Average the duplicate readings for each Standard Solution, control, and sample and subtract the
average zero standard optical density. Plot the optical density for the standards versus the concentration
of the standards and draw the best curve. The data can be linearized by using log/log paper and
regression analysis may be applied to the log transformation. To determine the human S100A11
concentration of each sample, first find the absorbance value on the y-axis and extend a horizontal line
to the standard curve. At the point of intersection, extend a vertical line to the x-axis and read the
corresponding human S100A11 concentration. If the samples have been diluted, the concentration read
from the standard curve must be multiplied by the dilution factor.
1. The dose-response curve of this assay fits best to a sigmoidal four-parameter logistic equation. The
results of unknown samples can be calculated with any computer program having a four-parameter
logistic function. It is important to make an appropriate mathematical adjustment to accommodate for
the dilution factor.
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2. Most microtiter plate readers perform automatic calculations of analyte concentration. The calibration
curve is constructed by plotting the absorbance (Y) of calibrators versus log of the known
concentration (X) of calibrators, using the four-parameter function. Alternatively, the logit log
function can be used to linearize the calibration curve (i.e. logit of absorbance (Y) is plotted versus
log of the known concentration (X) of calibrators).
Measurement Range
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The measurement range is 15.6 pg/mL to 1,000 pg/mL. Any sample reading higher than the highest
standard should be diluted with Dilution Buffer in higher dilution and re-assayed. Dilution factors need
to be taken into consideration in calculating the human S100A11 concentration.
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
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Troubleshooting
1. The Standard Solutions should be run in duplicate, using the protocol described in the Detailed
Protocol. Incubation times or temperatures significantly different from those specified may give
erroneous results.
2. Poor duplicates, accompanied by elevated values for wells containing no sample, indicate insufficient
washing. If all instructions in the Detailed Protocol were followed accurately, such results indicate a
need for washer maintenance.
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3. Overall low signal may indicate that desiccation of the plate has occurred between the final wash and
addition of Substrate Reagent. Do not allow the plate to dry out. Add Substrate Reagent immediately
after wash.
Reagent Stability
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All of the reagents included in the CycLex Research Product CircuLex S100A11 ELISA Kit have
been tested for stability. Reagents should not be used beyond the stated expiration date. Upon receipt, kit
reagents should be stored at 4°C, except the reconstituted S100A11 Standard must be stored at below
-70°C. The Microplate should be stored in the original foil bag sealed by the zip lock and containing a
desiccant pack.
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S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
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Assay Characteristics
1. Sensitivity
The limit of detection (defined as such a concentration of human S100A11 giving absorbance higher
than mean absorbance of blank* plus three standard deviations of the absorbance of blank: A blank +
3SD blank) is better than 4.34 pg/mL of sample.
* Dilution Buffer is pipetted into blank wells.
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Typical Standard Curve
S100A11 Standard Curve
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2.5
2.0
A450
1.5
1.0
0.0
200
400
600
800
1,000
1,200
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0
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0.5
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S100A11 conc. (pg/mL)
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Cat#: CY-8063
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
2. Precision
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Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay
precision.
• Intra-assay (Within-Run, n=16) CV=2.1-3.2 %
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S100A11 conc. (pg/ml)
Sample 2
Sample 3
169.5
337.8
168.9
359.0
174.5
357.2
176.4
369.6
178.4
360.8
174.7
363.4
179.0
355.9
181.0
360.4
172.5
338.3
170.5
353.1
170.9
363.6
175.7
359.4
170.9
359.6
171.8
358.0
174.6
356.3
176.5
363.3
181.0
369.6
168.9
337.8
174.1
357.2
3.60
8.41
2.1%
2.4%
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1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
MAX.
MIN.
MEAN
S.D.
C.V.
Sample 1
78.7
85.4
90.4
85.9
87.9
83.2
82.1
83.5
83.1
86.5
87.8
86.6
86.0
85.2
83.6
84.3
90.4
78.7
85.0
2.72
3.2%
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Cat#: CY-8063
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
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Inter-assay Precision (Precision between assays)
Tree samples of known concentration were tested in five separate assays to assess inter-assay
precision.
• Inter-assay (Run-to-Run, n=5) CV=6.3-10.7 %
S100A11 conc. (pg/ml)
Sample 2
Sample 3
185.6
345.5
205.6
407.8
196.6
426.6
206.8
466.0
178.7
414.2
206.8
466.0
178.7
345.5
194.7
412.0
12.3
43.5
6.3%
10.6%
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Sample 1
90.7
112.2
89.4
94.4
86.9
112.2
86.9
94.7
10.2
10.7%
1
2
3
4
5
MAX.
MIN.
MEAN
S.D.
C.V.
3. Linearity
Two samples were diluted with Dilution Buffer and assayed after dilution. The neat sample is set to 1.
Please note that all samples including the neat sample are 8-fold diluted as stated in the Assay Procedure.
The results are summarized in the figure below.
Line arity
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Sample 1
Sample 2
500
400
300
200
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100
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S100A11 conc. (pg/ml)
600
0
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Cat#: CY-8063
0.2
0.4
0.6
0.8
1
1.2
Sample Dilution ratio
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Version#: 140318
Example of Test Results
Fig.1 S100A11 Level in conditioned media of several cell lines
S100A11 conce ntration in conditione d me dium
180
140
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S100A11 conc. (ng/mL)
160
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S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
TM
120
100
80
60
40
0
SW480
He La
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20
A431
LOVO colo205
PC3
DU145
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Fig.2 S100A11 concentration in cell lysates (ca. 1 mg/mL protein concentration) from many cell lines
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S100A11 concentration in cell lysates
1.6
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1.2
1.0
0.8
0.6
0.4
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S100A11 conc.(ng/ml)
1.4
0.2
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
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Fig.3 Serum S100A11 level in 20 CRP-positive volunteers and 20 healthy volunteers.
Average S100A11 concentration in sera
CRP-positive volunteers: 1,091.6 pg/mL (n=20)
Healthy volunteers: 526.3 pg/mL (n=20)
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3,000
2,000
1,500
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S100A11 conc. (pg/mL)
2,500
1,000
500
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0.5
1
1.5
CRP-positive
2
Normal
2.5
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Cat#: CY-8063
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Version#: 140318
S100A11 ELISA Kit
User’s Manual
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References
Related Products
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1. Todoroki H, Kobayashi R, Watanabe M, Minami H, Hidaka H.; Purification, characterization, and
partial sequence analysis of a newly identified EF-hand type 13-kDa Ca2+-binding protein from
smooth muscle and non-muscle tissues. J Biol Chem 266: 18668-18673, 1991
2. Ohta H, Sasaki T, Naka M, Hiraoka O, Miyamoto C, Furuichi Y, Tanaka T.; Molecular cloning and
expression of the cDNA coding for a new member of the S100 protein family from porcine cardiac
muscle. FEBS Lett 295: 93-96, 1991
3. Rety S, Osterloh D, Arie JP, Tabaries S, Seemann J, Russo-Marie F, Gerke V, Lewit-Bentley A.;
Structural basis of the Ca2+-dependent association between S100C (S100A11) and its target, the
N-terminal part of annexin I. Structure 8: 175-184, 2000
4. Ning Chang, Cindy Sutherland, Eva Hesse, Robert Winkfein, William B. Wiehler, Mark Pho, Claude
Veillette, Susan Li, David P. Wilson, Eniko Kiss, and Michael P. Walsh.; Identification of a novel
interaction between the Ca2+-binding protein S100A11 and the Ca2+-and phospholipid-binding
protein annexin A6. Am J Physiol Cell Physiol 292: C1417-C1430, 2007
5. Zhao XQ, Naka M, Muneyuki M, Tanaka T.; Ca2+-dependent inhibition of actin-activated myosin
ATPase activity by S100C (S100A11), a novel member of the S100 protein family. Biochem Biophys
Res Commun 267: 77-79, 2000.
6. Ruse M, Lambert A, Robinson N, Ryan D, Shon KJ, Eckert RL.; S100A7, S100A10, and S100A11
are transglutaminase substrates. Biochemistry 40: 3167-3173, 2001.
7. Deloulme JC, Assard N, Mbele GO, Mangin C, Kuwano R, Baudier J.; S100A6 and S100A11 are
specific targets of the calcium- and zinc-binding S100B protein in vivo. J Biol Chem 275:
35302-35310, 2000.
8. Sakaguchi M., Sonegawa H., Nukui T., Sakaguchi Y., Miyazaki M., Namba M., Huh N. H. ;
Bifurcated converging pathways for high Ca2+- and TGFbeta-induced inhibition of growth of normal
human keratinocytes. Proc. Natl. Acad. Sci. USA 102: 13921-13926, 2005
9. Sakaguchi M., Miyazaki M., Takaishi M., Sakaguchi Y., Makino E., Kataoka N., Yamada H., Namba
M., Huh N. H.; S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human
epidermal keratinocytes. J. Cell Biol 163: 825-835, 2003
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* CircuLex S100A13 ELISA Kit: Cat# CY-8057
* CircuLex S100A12 ELISA Kit: Cat# CY-8058
* CircuLex S100P ELISA Kit: Cat# CY-8060
* CircuLex S100A8-MRP8 ELISA Kit: Cat# CY-8061
* CircuLex S100A9-MRP14 ELISA Kit: Cat# CY-8062
* CircuLex S100A11 ELISA Kit: Cat# CY-8063
* CircuLex S100A14 ELISA Kit: Cat# CY-8064
* CircuLex S100A7/Psoriasin ELISA Kit: Cat# CY-8073
* CircuLex S100A4 ELISA Kit Ver.2: Cat# CY-8086
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* Anti-Human S100A3 (Clone YK-3E3): Cat# CY-M1039
* Anti-Human S100A4 (p9Ka): Cat# CY-P1026
* Anti-Human S100P: Cat# CY-P1028
* Anti-Human S100A10: Cat# CY-P1033
* Anti-Human S100A16: Cat# CY-P1034
* Anti-Human S100A3: Cat# CY-P1039
* Anti-Human S100A2: Cat# CY-P1040
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Cat#: CY-8063
16
Version#: 140318
S100A11 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
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CircuLex
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* Human S100B: Cat# CY-R2250
* Human S100A1: Cat# CY-R2251
* Human S100A2: Cat# CY-R2252
* Human S100A3: Cat# CY-R2253
* Human S100A4: Cat# CY-R2254
* Human S100A5: Cat# CY-R2255
* Human S100A6: Cat# CY-R2256
* Human S100A7: Cat# CY-R2257
* Human S100A8: Cat# CY-R2258
* Human S100A9: Cat# CY-R2259-G
* Human S100A9: Cat# CY-R2259-H
* Human S100A10: Cat# CY-R2260
* Human S100A12: Cat# CY-R2262-G
* Human S100A12: Cat# CY-R2262-H
* Human S100A13: Cat# CY-R2263
* Human S100A14: Cat# CY-R2264
* Human S100A16: Cat# CY-R2266
* Human S100P: Cat# CY-R2267
* Human S100A11: Cat# CY-R2269
PRODUCED BY
er
en
CycLex Co., Ltd.
1063-103 Terasawaoka
Ina, Nagano 396-0002
Japan
Fax: +81-265-76-7618
e-mail: info@cyclex.co.jp
URL: http://www.cyclex.co.jp
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* Human S100A1 Low Endotoxin: Cat# CY-R2451
* Human S100A3 Low Endotoxin: Cat# CY-R2453
* Human S100A4 Low Endotoxin: Cat# CY-R2454
* Human S100A7 Low Endotoxin: Cat# CY-R2457
* Human S100A8 Low Endotoxin: Cat# CY-R2458
* Human S100A9 Low Endotoxin: Cat# CY-R2459-G
* Human S100A11 Low Endotoxin: Cat# CY-R2461
* Human S100A12 Low Endotoxin: Cat# CY-R2462-G
* Human S100A14 Low Endotoxin: Cat# CY-R2464
* Human S100P Low Endotoxin: Cat# CY-R2467
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CycLex/CircuLex products are supplied for research use only. CycLex/CircuLex products and
components thereof may not be resold, modified for resale, or used to manufacture commercial
products without prior written approval from CycLex Co., Ltd.. To inquire about licensing for
such commercial use, please contact us via email.
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Cat#: CY-8063
17
Version#: 140318