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Richter Optica
[email protected]
Instructions for Models:
U1B, U1T, U1D, U1LCD
Universty Student Biological Microscope
Trinocular Port
Diopter Adjustment
Ring
Objective
Moveable Finger
Specimen Slide
Holder
Condenser locking screw
Coarse Focus
Fine Focus
Condenser Control
Knob
Swing out Filter
Holder
Interpupillary Adjustment
Scale
Eyepiece
Head Locking Screw
Revolving Nosepiece
Set Screws to secure specimen holder to stage
Stage
Abbe Condenser
X-Axis Control
Knob
Y-Axis Control
Knob
Iris diaphragm lever
Lamp Housing
Thank you for your purchase of a Richter Optica microscope. The information in this manual is provided to answer most questions that can arise when operating your microscope and to help you avoid unneccesary maintenance expenses in the future.
Please carefully read instructions before operating microscope. Nomenclature used to describe components and controls are identified on opposite page.
UNPACKING
Do not discard styrofoam container or packing materials until you are sure shipment is complete and undamaged (retain styrofoam shipping container to store your microscope when it is not in use). Remove all tape and packing material used to protect microscope during shipment. Make certain lens surfaces do not come in contact with dirt, fingerprints or oil.
Damage of lens surfaces occur when they come in contact with such contaminants, and image quality is reduced.
OPERATION
A. Illumination
1. Before operating microscope, adjust intensity control located on side of base to the minimum position. This should be done prior to each time the base is turned on or off. This will extend bulb
life.
2. Insert power plug into base of microscope. Then insert plug into outlet.
3. Push rocker switch at back of the base to on position.
4. Rotate intensity dial on illuminator base until the image is illuminated.
B.
5. Adjust intensity of light to match requirements of objective and specimen slide.
Interpupillary Adjustment of Viewing Head
1. Looking through microscope, adjust distance between the two eyepieces by grasping the eyetubes and moving them either closer or farther apart.
C.
2. When a full field of view is observed throught both tubes, and images blend into one, interpupillary distance is corrected for your eyes. Check the interpupillary scale and note index reading for future reference, in case other users will be changing this adjustment from time to time.
3. Adjust the diopter scales, located on each eyetube, to the same numerical value as indicated on the interpupillary scale. This must be done in order to maintain parfocality of objective lenses. If interpupillary distance is changed, adjust eyepiece diopters accordingly.
Focusing the Microscope
1. Position the 4x objective lens into the optical path, making sure that lens clicks into its proper position.
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2. Place standard specimen slide (cover slip up) on top of stage surface.
a. Swing movable finger on slide holder outward. Place specimen slide against fixed side of slide
holder. Slowly release moveable finger until it makes contact with the specimen slide.
3. Rotate coarse focusing controls until the specimen comes into focus.
4. Adjust fine focus controls until specimen is in sharp focus.
5. Adjust diopter for difference in eyesight.
a. Using right eye, peer into the right eyepiece tube. Adjust sharpness of image by turning diopter
adjustment located on left eyepiece tube.
6. Adjust the aperture (opening) of the iris diaphragm.
Iris diaphragm should not be used to control the brightness of illumination, use light intensity control
knob to adjust light level. Iris diaphragms are designed to help achieve high resolution of specimen and
provide contrast in the image. Smaller apertures will deliver contrast to the image. However, closing
aperture too much will reduce resolution. Experimentation is the best method of determining the
correct opening of the diaphragm. Some suggested openings for the iris diaphragm are:
Objective Diaphragm Opening
4x
10x
40x
100x
1/8 open
1/8 to 1/4 open
1/4 to 1/2 open
1/2 to 3/4 open
7. Changing magnification.
a. Rotate revolving nosepiece to postition 10x objective into optical path.
b. This microscope has been parfocalized, which allows changes from one objective to another
while requiring only a slight adjustment of the fine focus controls.
c. When changing to the 40x and 100x objective lens, care must be exercised in order to prevent
damaging the front lens element and specimen slide.
d. In order to obtain the maximum resolution of the 100x oil immersion lens, it is necessary to
apply immersion oil between the cover glass of slide and the front lens of the objective.
1.) Use a very small amount of immersion oil. Only the tip of the lens should ever come in
contact with the oil.
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1. Optical Maintenance a. Do not attempt to disassemble any lens component. Consult an expert technical service company when repairs not covered by these instructions are required.
b. c.
Prior to cleaning any lens surface, brush dust or dirt off lens surfaces using a camel hair brush. Or use air to blow dust and lint off surfaces. Compressed air, available at any computer supply store, is a good source of clean air.
Cleaning eyepiece lenses.
d.
Do not remove eyepiece from eyepiece tube. Clean only the outer surface. Breathe on lens to dampen surface, then wipe with lens paper. Do not wipe lens surface while dry as lenses are scratched very easily. Wipe in a circular motion from center to outer edges.
Cleaning objective lenses.
e. f.
Do not remove objective lenses from the microscope. Clean front lens element only. Using a cotton swab saturated with distilled water, clean front lens surface. Inspect the lens using a magnifying glass to ensure that the element is clean. If immersion oil or specimen material of any kind is evident, use a cotton swab dipped in a small amount of Windex to clean all foreign material from objective lens surface. Such material will reduce or totally block image quality.
Cleaning condenser lens.
Clean only the top lens surface, visible when looking through hole in top of stage. Use same procedure as used for eyepiece or objective lenses.
Illuminator condenser lens.
Use same procedure as used for eyepiece or objective lenses.
2. Electrical Maintenance
WARNING: FOR YOUR SAFETY, TURN SWITCH OFF AND REMOVE PLUG FROM POWER SOURCE OUTLET BEFORE
MAINTAINING
1. Replacement of Bulb a. Carefully pull magnetic lamp housing up and off of the microscope to reveal the bulb.
b. c. d. e.
Using a tissue or cloth gently grasp the halogen bulb, pull straight out of lamp socket.
Your microscope requires an LED bulb available from your microscope dealer.
Make certain that the new bulb is clean, as fingerprints on the bulb can affect light transmission.
Grasping bulb gently with a tissue or cloth, insert pins straight into the lamp socket.
Replace magnetic lamp housing.
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2. Replacement of Fuse
The fuse is located on the back of the microscope on the right side of the power plug. To remove the fuse from the holder, insert a 6mm screwdriver blade into slot located in rear of fuse cap.
Slightly depress and rotate screwdriver to release the fuse cap. Pull cap and fuse out of fuse holder. Insert proper 1amp electronic fuse into fuse cap. Using screwdriver, rotate fuse cap assembly in opposite direction of arrow until guide slot engages, depress fuse cap and rotate
1/4 turn to lock into fuse.
TROUBLESHOOTING
ELECTRICAL
Problem
Light Fails to Operate
Reason for Problem
Outlet inoperative
AC Power cord not connected
Bulb burned out
Fuse burned out
Fuse burns out too soon
Fuse blows instantly when replaced.
Solution
Have qualified service technician repair outlet plug into outlet
Replace with new LED bulb
Replace with new 1 amp fuse
Replace with proper 1 amp fuse
Unit has short, have qualified service technician repair electrical short
Problem
Light burns out too soon
Light bulb burns out immediately
Light Flickers
Reason for Problem
The voltage is too high
Incorrect lamp used
Lamp not properly inserted into socket
Lamp about to burn out
Fuse holder not locked into proper position
Loose connection at AC outlet
Solution
Adjust intensity control to the minimum position before turning the power switch on.
Have qualified service technician use proper bulb. Plug unit into proper outlet
120v or 220v.
Have qualified service technician make repairs.
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0.0
0.2
0.8
1.0
TROUBLESHOOTING
Problem
No Image
Poor Resolution (Image not sharp)
Spots in field of view
Uneven illumination of field
IMAGE QUALITY
Reason for Problem
Nosepiece not indexed properly
Light too bright
Objective lens dirty
Eyepiece lens dirty
Slide upside down
Cover slip on specimen slide too thick
Too much light
Condenser lens dirty
Rack stop not set at a proper position
Eyepiece dirty
Specimen slide dirty
Condenser lens dirty
Nosepiece not properly indexed
Diaphragm not properly indexed
Solution
Move revolving nosepiece until objective lens clicks into position.
Adjust light intensity control to a lower position.
Clean objective lens
Clean eyepiece lens
Turn specimen slide over cover slip facing up
Use 0.17mm thick cover slip
Adjust light intensity control to a lower position.
Clean condenser lens
Adjust rack stop
Clean eyepiece lens
Clean slide
Clean lens of condenser
Revolve nosepiece into positive index stop
Adjust diaphragm to proper level
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Problem
Does not stay in focus
MECHANICAL PROBLEM
Reason for Problem
Stage drops down
Solution
Adjust tension adjustment knob
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