Hematocrit Centrifuge Model 410E User Manual

Hematocrit Centrifuge
Model 410E
User Manual
Read this manual before operating the 410E Hematocrit
Centrifuge. Save this manual for future reference.
The most current version of this manual can be found
online at www.grahamfield.com.
410E-INS-LAB-RevC15
CONTENTS
INTRODUCTION.................................................................................................... 2
PRODUCT OVERVIEW.................................................................................... 3
GLOSSARY...................................................................................................... 3
INTENDED USE............................................................................................... 4
IMPORTANT SAFETY GUIDELINES—PLEASE READ BEFORE USE............... 4
SETUP.................................................................................................................... 5
UNPACKING.................................................................................................... 5
CONTENTS...................................................................................................... 6
REPLACEMENT PARTS................................................................................. 6
INSTALLATION................................................................................................ 6
SPECIMEN COLLECTION.................................................................................... 7
MATERIALS NEEDED:.................................................................................... 7
CAPILLARY TUBE SPECIFICATIONS............................................................ 7
TUBE PREPARATION..................................................................................... 7
OPERATION.......................................................................................................... 7
BALANCED LOADS........................................................................................ 7
LOADING THE ROTOR................................................................................... 8
SETTING THE DIGITAL TIMER....................................................................... 8
OPERATING THE CENTRIFUGE.................................................................... 9
INTERPRETING RESULTS: USING THE READING SCALE............................... 9
EXPECTED VALUE....................................................................................... 10
LIMITATIONS AND FACTORS AFFECTING RESULTS............................... 10
DISASSEMBLY.................................................................................................... 11
TROUBLESHOOTING......................................................................................... 11
MAINTENANCE................................................................................................... 12
CLEANING..................................................................................................... 12
TRANSPORTATION....................................................................................... 13
STORAGE...................................................................................................... 13
SPECIFICATIONS................................................................................................ 14
LIMITED WARRANTY......................................................................................... 15
GF Health Products, Inc. is not responsible for typographical errors. For the most
updated and current information on packaging, warranties, products and specifications,
including the most current version of these instructions, please visit our website
at www.grahamfield.com. Graham-Field and Grafco are registered trademarks of
GF Health Products, Inc.
INTRODUCTION
Your Grafco Hematocrit Centrifuge has been designed to
be reliable, safe, and easy to use. This manual contains
important operation and safety information. Carefully
read and understand the contents of this manual before
installing or using the Grafco Hematocrit Centrifuge.
Always install this centrifuge in accordance with local
electrical parameters.
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Product Overview
timer LED
digital display
lid
locking
button
brake
switch
unlocking
button
timer set button
(ten minute
increments)
spin start button
ON/OFF
switch
timer set button
(one minute increments)
410E Hematocrit Centrifuge
The Grafco Hematocrit Centrifuge, shown above, features a
digital timer (up to 99 minutes), fixed speed, brake control,
and a reading scale. This unit spins loads at a fixed speed
of 12,000 RPM and has a thermal cutout switch to prevent
overheated operating temperature. The Grafco Hematocrit
Centrifuge can hold up to 24 capillary tubes of size 1.2mm
(or 1.5mm) x 75mm.
Centrifugation of anticoagulated whole blood results in the
packing of red blood cells. The hematocrit reading is the
percentage of the volume of packed red cells in relation
to the volume of whole blood. The hematocrit tube is
filled with anticoagulated whole blood, one end sealed by
heat or insertion into a sealing clay, and then centrifuged.
Interpretation of the resulting Volume of Packed Red Cells
(VPRC) may be used to detect the presence of anemia or
polycythemia.
Glossary
Hematocrit Centrifuge: An instrument which uses
centrifugation to measure the ratio of the volume of red
blood cells to the total volume of blood.
G Force: The measurement for samples undergoing the
stress of acceleration in a centrifuge.
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RCF (Relative Centrifugal Force): The centrifugal force
that the samples undergo, which relies on the speed
of rotation (N) in RPM and the rotating radius (R). The
rotating radius is measured from the center axis of the
rotor to the extreme ends of the centrifuge tubes.
RCF Formula: RCF = 11.18 x R x (N/1000)²
Intended use
The Grafco Hematocrit Centrifuge is intended to provide
laboratory tests for hematocrit values (HCT) in the fields
of biochemistry, microbic immunity genetics, and blood
separation, as well as general clinic tests.
IMPORTANT SAFETY GUIDELINES—PLEASE
READ BEFORE USE
The safety statements presented in this chapter refer to the
basic safety information that the operator of the centrifuge
shall pay attention to and abide by. There are additional safety
statements in other chapters or sections, which may be the
same as or similar to the following, or specific to the operations.
WARNING: Indicates a potential hazard situation or
unsafe practice that, if not avoided, could result in
death or serious personal injury.
CAUTION: Indicates a potential hazard or unsafe
practice that, if not avoided, could result in moderate
or minor personal injury.
sNOTICE: Indicates a potential hazard or unsafe
practice that, if not avoided, could result in product or
property damage.
Info: Provides application recommendations or other useful
information to ensure that you get the most from your product.
WARNING: Important! Read and understand these
instructions before installing or using the Grafco
410E Hematocrit Centrifuge. If you do not understand
any part of these instructions, contact your medical
professional or GF authorized distributor for direction
in the use of this product.
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WARNING: For the safety of both the operator
and service personnel, take care when handling
substances that are known to be toxic, radioactive or
contaminated with pathogenic microorganisms when
using this centrifuge.
WARNING: If components are damaged or missing,
contact your GF authorized distributor immediately.
DO NOT use substitute parts.
WARNING: Notice for California Customers- California
Proposition 65 WARNING: This product contains a
chemical known to the State of California to cause
cancer and reproductive or developmental harm.
WARNING: GF Health Products, Inc. assumes no
responsibility for any damage or injury caused by
improper installation or use of this product.
SETUP
Unpacking
1. Check for obvious damage to the carton or its contents.
If damage is evident, please notify the carrier and GF
Health Products, Inc.
2. Open the product box carefully. This may include
cutting external packaging strips which surround the
product shipping box. Also, for added safety when
shipping, the centrifuge may sometimes be packaged
inside the outer shipping box, meaning two boxes must
be opened.
3. Carefully remove the Hematocrit Centrifuge from the
packaging, including removing the plastic bag in which
the centrifuge is placed for shipping purposes. Do not
drop the centrifuge. Ensure that all of the equipment
listed in Contents, following, is present.
4. Keep the packing materials for transportation and/or
storage.
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Contents
Your Grafco Hematocrit Centrifuge contains:
Equipment
supplied
with 410E
Quantity
1
Description
Rotor with lid
1
Detachable power cord
1
Reader scale
Replacement parts
Replacement parts available for the Grafco 410E
Hematocrit Centrifuge (from your GF authorized
distributor): Grafco Item 410E-1, Replacement Carbon
Brushes, package of two.
Installation
sNOTICE: The operation of AC motor is affected by
the surrounding power consumption, which will
result in a fluctuation of speed. To assure an accurate
RPM and its corresponding RCF, do not place this
equipment in draft, sunlight or near a piece of
equipment that emits heat and/or electromagnetic
conduction emission.
1. Place the centrifuge in a large, open space with
unencumbered air flow on a sturdy, flat, level countertop
with sufficient clearance to open the lid completely.
2. Attach the product's electrical cord to the cord port
located on the back of the centrifuge.
3. Plug the centrifuge's power cord into a properly
grounded (110V / 60Hz) electrical wall outlet.
sNOTICE: Locate the unit so that the centrifuge will
not be accidentally pulled or dislodged from the
countertop; and so the cord will not be stepped on,
tripped over, or otherwise subjected to damage or
stress.
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SPECIMEN COLLECTION
Materials Needed:
Capillary tubes
If non-anticoagulated blood is used for the procedure, we
recommend the use of heparinized capillary tubes
If blood is already anticoagulated, we recommend the use of
plain capillary tubes
Clay sealant
Centrifuge with rotor which will accept capillary tubes and attain at least
11,500 RPM
Chart for determining hematocrit values
Capillary Tube Specifications
Capillary Tubes Heparinized:
Plain
Precalibrated:
Heparinized
I.D. 1.1mm – 1.2mm, wall 0.2mm + 0.02mm,
length 75mm
I.D. 0.5mm – 0.6mm, O.D. 1.4mm – 1.7mm,
length 75mm
Calibration line 60mm from one end
Tube Preparation
1. Using 2 capillary tubes, insert an end of each into the
blood, tilt slightly and allow the tubes to fill by capillary
attraction to 2/3 – 3/4 full.
2. Create a sealing plug: Insert the dry ends of the tubes into
the sealing clay and withdraw them with a twisting motion.
OPERATION
Balanced Loads
sNOTICE: Your centrifuge must contain a balanced
load in order to work properly. Spinning unbalanced
loads may shorten the life of the device and produce
unpredictable results. Observe the following rules
when loading the rotor.
• Opposing rotor grooves must be empty or loaded with
equally weighted samples.
• If an odd number of samples is to be spun, fill a tube
with water to match the weight of the unpaired sample
and place it across from the unpaired sample.
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Loading the Rotor
The Grafco Hematocrit Centrifuge can hold up to 24
capillary tubes of size 1.2mm (or 1.5mm) x 75mm.
1. Open the centrifuge lid (if centrifuge lid is locked, press
the unlocking button on front of centrifuge to unlock it).
2. Turn the rotor lid knob counter-clockwise to unlock the
it. Open the rotor lid.
3. Carefully place the test tubes in the rotor grooves,
ensuring the capillary tubes touch the rotor's outer
rubber gasket.
4. Make sure the load is balanced by placing similar tubes
across from one another. This will keep the rotor in
balance (see previous section, Balanced Loads).
5. Close the rotor lid gently. Turn the knob clockwise to
lock it.
WARNING: Closing the rotor lid improperly may result
in broken capillary tubes, which could cause injury.
6. Close the centrifuge lid completely. Press the locking
button on the side of the centrifuge to lock the lid.
7. Turn the ON/OFF switch to ON position.
Setting the Digital Timer
• The Grafco Hematocrit Centrifuge is equipped with an
LED digital display timer which allows the user to spin
the load from 1 to 99 minutes.
• Press the triangle directly below the left side of the
timer to change the spin time in increments of ten (10)
minutes.
• Press the triangle directly below the right side of the
timer to change the spin time in single minutes.
• For example, if the desired spin time is twenty five
minutes, press the left arrow twice to display a 2 on the
timer and press the right arrow five times to display a
five on the timer.
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Operating the Centrifuge
WARNING: As a safety feature, the centrifuge
has a power shut-off device to turn the power off
automatically when the lid is opened during spinning.
This is a safety feature of the centrifuge. DO NOT
open the lid when the rotor is spinning.
1. After the desired spin time is displayed on the timer,
press the START button to initiate rotor spinning.
2. The timer will display the spin time remaining during
operation. When the spin cycle is complete, the unit will
slowly stop spinning.
3. To accelerate the deceleration, press the BRAKE SWITCH.
4. Once the rotor has completely stopped spinning, turn
the ON/OFF switch to OFF position.
5. Press the unlocking button located on the front of the
centrifuge to unlock the lid. Open the centrifuge lid.
6. Carefully open the rotor lid and remove the specimens.
WARNING: DO NOT open the centrifuge lid until the
rotor has completely stopped spinning. Opening the
lid before the rotor has stopped spinning could result
in personal injury.
INTERPRETING RESULTS: USING THE READING
SCALE
100%
90
80
tube
holder
capillary
tube
centrifuge
adjustable bar
capillary tube
70
diagonal
line
60
50
extreme end
40
30
leucocyte
20
demarcation
10
red blood cells
0
scale
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1. Remove the capillary tubes from the rotor. Take care
not to let the blood drain from the tubes.
2. Place the capillary tubes on the grooves of the tube
holder carefully. Line up the bottom red blood cell
portion of the tube with the straight line at the bottom
of the reading scale so that the start of the red blood
cell portion lines up with this horizontal fixed line.
3. Move the tube holder from left to right on the reader
until the top of the leucocyte line matches up with the
fixed diagonal line on the reader platform.
4. Move the adjustable bar to meet the demarcation (place
where the leucocyte and red blood cells separate). The
diagonal line on the adjustable bar should be directly on
top of the demarcation.
5. The percentage of HCT can then be read directly from
the scale by reading the number the adjustable diagonal
bar crosses at the right side of the reader.
Expected Value
Normal hematocrit values will vary somewhat depending
upon such factors as age, sex, size of red cells, and
altitude. Generally accepted normal values with range of
variation are:
• Women: 36% – 46%
• Men: 42% – 52%
Limitations and Factors Affecting Results
1. Inadequate centrifugation, either time or RPM, will
result in erroneously high reading.
2. Ensure that the capillary tube is sealed properly, to
prevent red cell loss during centrifugation. The rubber
gasket encircling the interior of the rotor must be
smooth and pliable. This will aid in clay sealing plug
retention and reduce breakage during centrifugation.
3. Take the reading from the top of the clay sealing plug to
the top of the column of VPRC.
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4. Hemolized blood, due to over-anticoagulation or
improper finger stick, can produce erroneous results.
5. Hematocrit determinations are not accurate
immediately following blood transfusions or blood loss.
DISASSEMBLY
WARNING: Disassembly or dismantling of this
centrifuge is limited to authorized service personnel
only. Dismantling or disassembly by non-qualified
personnel will result in the voiding of the unit’s
warranty. Please contact your GF authorized
distributor or customer service representative to
identify a qualified technician.
TROUBLESHOOTING
WARNING: Unplug the centrifuge before performing
troubleshooting corrective actions.
WARNING: Do not disassemble or dismantle the
centrifuge.
Problem
Can’t turn
centrifuge
ON
Possible cause
1. Blown fuse
2. Disconnected electrical
cord
3. Worn carbon brushes
Corrective action
1. Contact GF authorized
distributor to replace fuse (10A)
2. Connect electrical cord
3. Contact GF authorized distributor
to replace carbon brushes
Erratic
speed
Worn carbon brushes
Contact GF authorized distributor
to replace carbon brushes
Vibration
Unbalanced rotor
Balance rotor load
Imbalance
1. Tubes are improperly
1. Balance load
positioned (unbalanced load) 2. Screw rotor knob to lock lid
2. Rotor lid is not locked
Broken test 1. Tubes are improperly
1. Balance load
tubes
positioned (unbalanced load) 2. Place tubes against rubber
2. Tubes are not placed all the
gasket on rotor
way down
3. Ensure rotor lid is placed flat
3. Rotor lid is tightened
against rotor
incorrectly
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MAINTENANCE
WARNING: Centrifuge disassembly or dismantling is
limited to authorized service personnel only.
WARNING: Always use a properly grounded electrical
outlet of correct voltage and current handling capacity.
WARNING: Do not immerse the centrifuge in or
saturate it with water.
sNOTICE: Do not lubricate the drive motor or linkage
assembly.
sNOTICE: Do not drop the centrifuge, turn it upside
down, or subject it to vibration.
sNOTICE: Clean the chamber and rotor as described
below after each use.
sNOTICE: In order to spin balanced loads, the
centrifuge must be level. To this end, confirm the
rubber supports are in good condition before each use.
sNOTICE: Contact your GF authorized distributor to
replace the carbon brushes yearly or as needed.
Cleaning
Clean the equipment as described below after each use.
1. Unplug the unit and disconnect the power cord from the
unit.
2. Clean the outer case, rotor lid, and rotor tube holder
grooves with a moist, clean cloth.
3. Ensure the equipment is thoroughly dry. Attach the
product's electrical cord to the cord port located on the
back of the centrifuge.
4. Plug the centrifuge's power cord into a properly
grounded (110V / 60Hz) electrical wall outlet.
sNOTICE: DO NOT use benzine or paint thinner for
cleaning.
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Transportation
sNOTICE: When transporting the unit, place in secure
packaging (preferably the original packaging material)
which will prevent the unit from tipping over.
sNOTICE: Do not drop the centrifuge, turn it upside
down, or subject it to vibration.
sNOTICE: Transport within temperature limitations
listed in following SPECIFICATIONS section.
Storage
sNOTICE: Do not place this equipment in a draft,
sunlight or near a piece of equipment which emits
heat or electromagnetic conduction emission.
sNOTICE: Disconnect power supply when the
centrifuge is not in use.
sNOTICE: DO NOT place other equipment or objects on
top of the centrifuge.
sNOTICE: Store within temperature limitations listed in
following SPECIFICATIONS section.
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SPECIFICATIONS
Model
CE marking
Grafco 410E Hematocrit Centrifuge
Conforms to LVD 73/23 EEC safety standards.
Standard
Complies with EC Electromagnetic Compatibility requirement
of EN60601-1-2.
EMI filter is designed in conformity with 89/336 EEC-EMC Directive.
Complies with IEC801 series regulations.
Complies with EN standard
ISO9001, ISO13485, GMP
N.W.
25.35 lb (11.5 kg)
Timer
Digital, 1 to 99 minutes
Motor input
Brush 80W
Brake
Manual
Rotor
F-2415
Capacity
24 capillary tubes
Max RCF
14490 x g
Speed RPM
Fixed 12,000 (though fixed at 12,000 RPM, motor is capable of
14,000 RPM)
Capillary tube size 1.1 – 1.2mm x 75 mm (length)
Conditions for
Transportation
and Storage
Temperature: 50ºF – 104ºF (10ºC – 40ºC)
Humidity: 40% – 90%
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LIMITED WARRANTY
SCOPE OF WARRANTY
GF Health Products, Inc. (“GF”) warrants to the original purchaser only that it will replace or
repair components, at GF’s sole discretion, that are defective in material or workmanship under
normal use and service. All warranties are conditioned upon the proper use of the products
strictly in accordance with good commercial practice and applicable GF instructions and
manuals, including proper use and maintenance. To the extent that a component is warranted
by a third party, GF conveys all of its rights under that warranty to the original purchaser, to
the extent permitted. This limited warranty shall only apply to defects that are reported to GF’s
customer service team within the applicable warranty period and which, upon examination by
GF or its authorized representative, prove to be a warranty item. This limited warranty is not
transferable. Within the guidelines set forth in this document, this product is warranted for one
(1) year. The applicable warranty period shall commence from date of shipment to the original
customer, unless there is an expiration date on the component in which case the warranty shall
expire on the earlier of warranty period or the expiration date.
OBTAINING WARRANTY SERVICE
This limited warranty shall only apply to defects that are reported to the Distributor from whom the
Customer purchased the product within the applicable warranty period. If there is not a Distributor,
you must contact GF directly by calling 770-368-4700, sending a fax request to 770-368-2386, or by
e-mailing a request to cs@grahamfield.com. Specific directions will be provided by the Customer
Service Representative. Failure to abide by the specific directions will result in denial of the
warranty claim.
EXCLUSIONS
The warranty does not cover and GF shall not be liable for the following:
1) Defects, damage, or other conditions caused, in whole or in part, by misuse, abuse,
negligence, alteration, accident, freight damage, tampering or failure to seek and obtain repair
or replacement in a timely manner;
2) Products which are not installed, used, or properly cleaned and maintained as required in the
official manual for the applicable product;
3) Products considered to be of a non-durable nature including, but not limited to: casters,
filters, fuses, gaskets, lubricants, and charts;
4) Accessories or parts not provided by GF;
5) Charges by anyone for adjustments, repairs, replacement parts, installation or other work
performed upon or in connection with such products which are not expressly authorized in
writing, in advance, by GF;
6) Any labor or shipping charges incurred in the replacement part installation or repair;
7) Costs and expenses of regular maintenance and cleaning; and
8) Representations and warranties made by any person or entity other than GF.
ENTIRE WARRANTY, EXCLUSIVE REMEDY AND CONSEQUENTIAL DAMAGES
DISCLAIMER
THIS WARRANTY IS GF’S ONLY WARRANTY AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESS
OR IMPLIED. GF MAKES NO IMPLIED WARRANTIES OF ANY KIND INCLUDING ANY IMPLIED
WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IF ANY MODEL
OR SAMPLE WAS SHOWN TO THE CUSTOMER, SUCH MODEL OR SAMPLE WAS USED MERELY TO
ILLUSTRATE THE GENERAL TYPE AND QUALITY OF THE PRODUCT AND NOT TO REPRESENT
THAT THE PRODUCT WOULD NECESSARILY CONFORM TO THE MODEL OR SAMPLE IN ALL
RESPECTS. THIS WARRANTY IS LIMITED TO THE REPAIR OR REPLACEMENT OF THE DEFECTIVE
PARTS. GF SHALL NOT BE LIABLE FOR AND HEREBY DISCLAIMS ANY DIRECT, SPECIAL, INDIRECT,
INCIDENTAL, EXEMPLARY OR CONSEQUENTIAL DAMAGES, INCLUDING, BUT NOT LIMITED TO:
DAMAGES FOR LOSS OF PROFITS OR INCOME, LOSS OF USE, DOWNTIME, COVER, OR EMPLOYEE
OR INDEPENDENT CONTRACTOR WAGES, PAYMENTS AND BENEFITS.
The warranties contained herein contain all the representations and warranties with respect
to the subject matter of this document, and supersede all prior negotiations, agreements and
understandings with respect thereto. The recipient of this document hereby acknowledges and
represents that it has not relied on any representation, assertion, guarantee, warranty, collateral
contract or other assurance, except those set out in this document.
For additional information on this product or this warranty, please contact a GF Customer Service
Representative.
NOTES:
1) Additional terms and conditions may apply.
2) Freight claims must be notated on the appropriate shipping documents and must be made
with immediacy. International, federal and state regulations govern specific requirements for
freight claims. Failure to abide by those regulations may result in a denial of the freight claim.
GF will assist you in filing the freight claim.
3) Claims for any short shipment must be made within three (3) days of the invoice date.
410E-INS-LAB-RevC15
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GF Health Products, Inc.
2935 Northeast Parkway
Atlanta, Georgia 30360
telephone: 770-368-4700
fax: 770-368-2386
www.grahamfield.com
Made in Taiwan
© 2008 GF Health Products, Inc.