Syntaxin 6 Antibody - Cell Signaling Technology

Store at –20°C
Syntaxin 6
Antibody
#2417
n3100 µl
Orders n 877-616-CELL (2355)
orders@cellsignal.com
Support n 877-678-TECH (8324)
info@cellsignal.com
Web n www.cellsignal.com
(10 western blots)
rev. 03/08/11
This product is intended for research purposes only. This product is not intended to be used for
therapeutic or diagnostic purposes in humans or animals.
Entrez-Gene ID #10228
Swiss-Prot Acc. #O43752
Applications
Species Cross-Reactivity*
Molecular Wt.
Source
W, IP
Endogenous
H, M, R, Mk
32 kDa
Rabbit**
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.
S
A
**Anti-rabbit secondary antibodies must be used to
detect this antibody.
CO
o2
12
PC
3T
3
ur
H/
Ne
NI
kDa
200
140
100
80
60
50
40
He
Background: Syntaxin 6 is a ubiquitously expressed S25C
family member of the SNARE proteins (1,2). The protein has
an amino-terminal H1 domain followed by an H2 SNARE
domain and a carboxy-terminal membrane anchor (3). Syntaxin 6 is localized at the trans-Golgi and within endosomes
and regulates membrane trafficking by partnering with a
variety of other SNARE proteins (3-5). Depending on cell
type and SNARE parter, syntaxin 6 has been found to be
involved in the regulation of GLUT4 trafficking, neutrophil
exocytosis and granule secretion (6-10).
k2
9
A1 3
72
*Species cross-reactivity is determined by Western blot.
Recommended Antibody Dilutions:
Western blotting
1:1000
Immunoprecipitation1:50
Syntaxin 6
30
Please visit www.cellsignal.com for a complete listing
of recommended companion products.
20
Specificity/Sensitivity: Syntaxin 6 Antibody detects
endogenous levels of total syntaxin 6 protein.
Source/Purification: Polyclonal antibodies are produced
by immunizing animals with a synthetic peptide (KLH-coupled) corresponding residue surrounding Tyr140 of mouse
syntaxin 6 protein. Antibodies are purified by protein A and
peptide affinity chromatography.
For application specific protocols please see the web
page for this product at www.cellsignal.com.
Background References:
(1) Bock, J.B. et al. (2001) Nature 409, 839–841.
10
Western blot analysis of extracts from various cell lines, using
Syntaxin 6 Antibody.
(2) Bock, J.B. et al. (1996) J. Biol. Chem. 271,
17961–17965.
(3) Wendler, F. and Tooze, S. (2001) Traffic 2, 606–611.
(4) Bock, J.B. et al. (1997) Mol. Biol. Cell 8, 1261–1271.
Ly
s
at
e
(5) Mallard, F. et al. (2002) J. Cell Biol. 156, 653–664.
7
+
(6) Perera, H.K. et al. (2003) Mol. Biol. Cell 14, 2946–2958.
#2
41
e
7
at
41
Ly
s
#2
kDa
© 2011 Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
200
140
100
80
60
50
40
(7) Shewan, A.M. et al. (2003) Mol. Biol. Cell 14, 973–986.
(8) Martín-Martín, B. et al. (2000) Blood 96, 2574–25783.
(9) Wendler, F. et al. (2001) Mol. Biol. Cell 12, 1699–1709.
IgG
30
(10) K uliawat, R. et al. (2004) Mol. Biol. Cell 15, 1690–1701.
Syntaxin 6
20
10
Western blot analysis of A172 lysate (lane1) and immunoprecipitates generated in the absence or presence of A172 cell lysate
(lanes 2 and 3), using Syntaxin 6 Antibody both as precipitation
and probing antibody.
IMPORTANT: For Western blots, incubate membrane with diluted antibody in 5% w/v w/v nonfat dry
milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Applications Key:
W—Western
Species Cross-Reactivity Key:
IP—Immunoprecipitation
H—human
M—mouse
Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans
IHC—Immunohistochemistry
R—rat
Hr—horse
Hm—hamster
ChIP—Chromatin Immunoprecipitation
Mk—monkey
Mi—mink
All—all species expected
C—chicken
IF—Immunofluorescence
F—Flow cytometry
Dm—D. melanogaster X—Xenopus
Z—zebrafish
E-P—ELISA-Peptide
B—bovine
Species enclosed in parentheses are predicted to react based on 100% homology.