Store at –20°C Syntaxin 6 Antibody #2417 n3100 µl Orders n 877-616-CELL (2355) firstname.lastname@example.org Support n 877-678-TECH (8324) email@example.com Web n www.cellsignal.com (10 western blots) rev. 03/08/11 This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. Entrez-Gene ID #10228 Swiss-Prot Acc. #O43752 Applications Species Cross-Reactivity* Molecular Wt. Source W, IP Endogenous H, M, R, Mk 32 kDa Rabbit** Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. S A **Anti-rabbit secondary antibodies must be used to detect this antibody. CO o2 12 PC 3T 3 ur H/ Ne NI kDa 200 140 100 80 60 50 40 He Background: Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (1,2). The protein has an amino-terminal H1 domain followed by an H2 SNARE domain and a carboxy-terminal membrane anchor (3). Syntaxin 6 is localized at the trans-Golgi and within endosomes and regulates membrane trafficking by partnering with a variety of other SNARE proteins (3-5). Depending on cell type and SNARE parter, syntaxin 6 has been found to be involved in the regulation of GLUT4 trafficking, neutrophil exocytosis and granule secretion (6-10). k2 9 A1 3 72 *Species cross-reactivity is determined by Western blot. Recommended Antibody Dilutions: Western blotting 1:1000 Immunoprecipitation1:50 Syntaxin 6 30 Please visit www.cellsignal.com for a complete listing of recommended companion products. 20 Specificity/Sensitivity: Syntaxin 6 Antibody detects endogenous levels of total syntaxin 6 protein. Source/Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding residue surrounding Tyr140 of mouse syntaxin 6 protein. Antibodies are purified by protein A and peptide affinity chromatography. For application specific protocols please see the web page for this product at www.cellsignal.com. Background References: (1) Bock, J.B. et al. (2001) Nature 409, 839–841. 10 Western blot analysis of extracts from various cell lines, using Syntaxin 6 Antibody. (2) Bock, J.B. et al. (1996) J. Biol. Chem. 271, 17961–17965. (3) Wendler, F. and Tooze, S. (2001) Traffic 2, 606–611. (4) Bock, J.B. et al. (1997) Mol. Biol. Cell 8, 1261–1271. Ly s at e (5) Mallard, F. et al. (2002) J. Cell Biol. 156, 653–664. 7 + (6) Perera, H.K. et al. (2003) Mol. Biol. Cell 14, 2946–2958. #2 41 e 7 at 41 Ly s #2 kDa © 2011 Cell Signaling Technology, Inc. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc. 200 140 100 80 60 50 40 (7) Shewan, A.M. et al. (2003) Mol. Biol. Cell 14, 973–986. (8) Martín-Martín, B. et al. (2000) Blood 96, 2574–25783. (9) Wendler, F. et al. (2001) Mol. Biol. Cell 12, 1699–1709. IgG 30 (10) K uliawat, R. et al. (2004) Mol. Biol. Cell 15, 1690–1701. Syntaxin 6 20 10 Western blot analysis of A172 lysate (lane1) and immunoprecipitates generated in the absence or presence of A172 cell lysate (lanes 2 and 3), using Syntaxin 6 Antibody both as precipitation and probing antibody. IMPORTANT: For Western blots, incubate membrane with diluted antibody in 5% w/v w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Applications Key: W—Western Species Cross-Reactivity Key: IP—Immunoprecipitation H—human M—mouse Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans IHC—Immunohistochemistry R—rat Hr—horse Hm—hamster ChIP—Chromatin Immunoprecipitation Mk—monkey Mi—mink All—all species expected C—chicken IF—Immunofluorescence F—Flow cytometry Dm—D. melanogaster X—Xenopus Z—zebrafish E-P—ELISA-Peptide B—bovine Species enclosed in parentheses are predicted to react based on 100% homology.