Technical data and operating instructions

Vivaspin ® Turbo 4 and 15

Vivaspin

®

Turbo 4 and 15 10K device for in vitro diagnostic use

Vivaspin

®

Turbo 4 and 15 3K, 5K, 30K, 50K, and 100K devices for research use only; not for use in diagnostic procedures

85032-541-63

85032-541-63

Vivaspin

®

Turbo 4 and 15 – Introduction

Storage conditions|shelf life

Vivaspin ® Turbo ultrafiltration spin columns should be stored at 15 – 30°C.

The devices should be used before the expiry date printed on the box.

Introduction

Vivaspin ® Turbo centrifugal concentrators offer the optimal solution to any concentration or buffer exchange application with their broad range of MWCOs prior to further sample analysis.

Vivaspin ® Turbo 4 is suitable for initial sample volumes of 2–4 ml and the Vivaspin ® Turbo

15 can handle samples up to 15 ml.

Highest flow rates are achieved due to their double vertical membranes which minimize protein polarization and subsequent fouling of the membrane. Additionally, their sleek internal profile ensures maximum process speeds right down to the last drops. The UV joining technology allows for a smooth joint transition between membrane and plastic housing, allowing complete sample recovery from the unique pipette friendly deadstop pocket. The dead stop pocket (patent pending) is an impermeable concentrate pocket integrally moulded below the membrane surface, thereby eliminating the risk of filtration to dryness.

– Vivaspin ® Turbo 4 & 15 10K device: 10,000 MWCO

– Vivaspin ® Turbo 4 & 15 30K device: 30,000 MWCO

– Vivaspin ® Turbo 4 & 15 50K device: 50,000 MWCO

– Vivaspin ® Turbo 4 & 15 100K device: 100,000 MWCO

Vivaspin ® Turbo 4 & 15 10K filter devices are for in vitro diagnostic use and can be used to concentrate serum, urine, cerebrospinal fluid, and other body fluids prior to analysis.

Vivaspin ® Turbo 4 & 15 3K, 5K, 30K, 50K, and 100K filter devices are for research use only and not for use in diagnostic procedures.

The Vivaspin use only.

® Turbo 4 and 15 devices are supplied non-sterile and are for single

Operation

Vivaspin ® Turbo 4 and 15 concentrators can be used in swing bucket or fixed angle rotors accepting standard conical bottom tubes. In a single spin, solutions can be concentrated up to 150 +. Samples are typically concentrated in 10 to 30 minutes with macromolecular recoveries in excess of 95%.

The Vivaspin ® Turbo 4 and 15 devices are the optimal solution for protein concentration from dilute samples e.g. after chromatography, cell culture supernatants or fermentation broths.

C

The Vivaspin ® Turbo product line includes 6 different cutoffs

(Molecular Weight Cutoff, MWCO):

– Vivaspin ® Turbo 4 & 15 3K device: 3,000 MWCO

– Vivaspin ® Turbo 4 & 15 5K device: 5,000 MWCO

Equipment Required

For use with centrifuge

1. Centrifuge with swing bucket or fixed angle rotor (minimum 25°).

2. Pasteur or fixed volume pipettes for sample delivery and removal.

Device

Vivaspin ® Turbo 4

Carrier Required

15 ml/17 mm d

Vivaspin ® Turbo 15 50 ml/30 mm d

2 |

Equipment Required

Equipment required Vivaspin ® Turbo 4

Centrifuge

Rotor type

Minimum rotor angle

Rotor cavity

Swing bucket Fixed angle

– 25°

To fit 15 ml (17mm) conical bottom tubes

Vivaspin ® Turbo 15

Swing bucket Fixed angle

– 25°

To fit 50 ml (30mm) conical bottom tubes

Rotor compatibility

Please note: Vivaspin ® Turbo 15 (30 mm +

118 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC

5 buckets and 368327 adaptors; Beckman

TA-10.250 25° fixed angle rotor with

356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes.

These devices are not designed to fit into rotors that only accept round bottom

29 mm + 105 mm tubes, e.g. Sorvall SS34 or Beckman JA 20.

| 3

Operation

1. Select the most appropriate membrane cut-off for your sample. For maximum recovery select a MWCO at least 50% smaller than the molecular size of the species of interest.

2. Fill concentrator with up to maximum volumes shown in table 1. (Ensure screw closure is fully seated)

3. Insert assembled concentrator into centrifuge (when fixed angle rotors are used, angle concentrator so that the printed window faces sidewards and the angular deadstop to the outside of the rotor).

4. Centrifuge at speeds recommended in table 2, taking care not to exceed the maximum g force indicated by membrane type and MWCO

5. Once the desired concentration is achieved, (see table 3a and 3b for guide to concentration times), remove assembly and recover sample from the bottom of the concentrate pocket with a pipette.

Desalting | Buffer Exchange

1. Concentrate sample to desired level.

2. Empty filtrate container.

3. Refill concentrator with an appropriate solvent.

4. Concentrate the sample again and repeat the process until the concentration of contaminating microsolute is sufficiently reduced. Typically, 3 wash cycles will remove

99% of initial salt content.

4 |

Technical Specifications

Table 1: Technical Specifications

Dimensions

Total Length

Width

Active membrane area

Hold up volume of membrane

Vivaspin

17 mm

3.2 cm 2

< 10µl

®

122.5 cm

Turbo 4 Vivaspin ® Turbo 15

118 mm in tube

27 mm

7.2 cm 2

<10 µl

100 µl Dead stop volume* in swing out 40 µl

Dead stop volume in fixed angle (25°)

Concentrator Capacity

Swing bucket rotor

30 µl

4 ml

4 ml Fixed angle rotor

Materials of construction

Concentrator insert

Concentrator cap and tube

Membrane

Styrene butadiene block

Polypropylene

60 µl

15 ml

9 ml

Styrene butadiene block co-polymer co-polymer

Polypropylene

Polyethersulfone Polyethersulfone

Table 2: Recommended Spin Speed (xg)

Vivaspin

Rotor

® Turbo 4 Centrifuge

Swing Bucket Fixed Angle

Membrane max max

3,000–50,000 MWCO PES 4,000 7,500

100,000 MWCO PES 3,000 5,000

Vivaspin

Rotor

® Turbo 15 Centrifuge

Swing Bucket Fixed Angle

Membrane max max

3,000–50,000 MWCO PES 4,000 4,000

100,000 MWCO PES 3,000 3,000

* Dead stop volume as designed in moulding tool. This volume may vary depending on sample, sample concentration, operation temperature and centrifuge rotor.

| 5

Usage Tips

1. Flow Rate

Filtration rate is affected by several parameters, including MWCO, porosity, sample concentration, viscosity, centrifugal force and temperature. Expect significantly longer spin times for starting solutions with over

5% solids. When operating at 4°C. flow rates are approximately 1.5 times slower than at 25°C. Viscous solutions such as 50%

glycerine will take up to 5 times longer to concentrate than samples in a predominantly buffer solution.

3. Sterilisation of Polyethersulfone

Membranes

Polyethersulfone membranes should not be autoclaved as high temperatures will substantially increase membrane MWCO.

To sterilise, use a 70% ethanol solution or sterilising gas mixture.

4. Chemical Compatibility

Vivaspin ® concentrators are designed for use with biological fluids and aqueous solutions.

For chemical compatibility details, refer to table 4.

2. Pre-rinsing

Membranes fitted to Vivaspin ® concentrators contain trace amounts of Glycerine and

Sodium azide. Should these interfere with analysis they can be removed by rinsing fill volume of buffer solution or deionised water through the concentrator. Decant filtrate and concentrate before processing sample solution. If you do not want to use the pre-rinsed device immediately, store it in the refrigerator with buffer or water covering the membrane surface. Please do not allow the membrane to dry out.

6 |

Performance Characteristics

Table 3a.: Performance Characteristics Vivaspin ® Turbo 4

Time to concentrate up to 20x [min.] at 20°C and solute recovery %

Rotor

Centrifugal speed

Start volume

Cytochrome c* (12,400 MW)

3 MWCO PES

Lysozyme* (14,300 MW)

3 MWCO PES

Cytochrome c* (12,400 MW)

5 MWCO PES

Lysozyme* (14,300 MW)

5 MWCO PES a-Chymotrypsin** (25,000 MW)

10 MWCO PES

BSA** (66,000 MW)

10 MWCO PES

30 MWCO PES

IgG** (160,000 MW)

30 MWCO PES

50 MWCO PES

100 MWCO PES***

Swing bucket

4,000 g

50

10

10

8

18

16

17

65

40

4 ml

Min.

60

Rec.

98%

95%

95%

94%

95%

98%

96%

94%

93%

94%

60

8

7

6

13

12

13

Fixed angle (25°)

7,500 g

70

50

4 ml

Min.

80

Rec.

96%

93%

94%

92%

95%

97%

97%

92%

90%

92%

* 0.25 mg/ml

** 1 mg/ml

*** 3,000 + g swing-out | 5000 + g fixed angle

| 7

Table 3b.: Performance Characteristics Vivaspin ® Turbo 15

Time to concentrate up to 20x [min.] at 20°C and solute recovery %

Rotor

Centrifugal speed

Start volume

Cytochrome c* (12,400 MW)

3 MWCO PES

Lysozyme* (14,300 MW)

3 MWCO PES

Cytochrome c* (12,400 MW)

5 MWCO PES

Lysozyme* (14,300 MW)

5 MWCO PES a-Chymotrypsin** (25,000 MW)

10 MWCO PES

BSA** (66,000 MW)

10 MWCO PES

30 MWCO PES

IgG** (160,000 MW)

30 MWCO PES

50 MWCO PES

100 MWCO PES***

Swing bucket

4,000 g

33

10

10

8

23

20

30

56

30

15 ml

Min.

61

Rec.

98%

98%

98%

96%

95%

99%

98%

95%

94%

92%

50

10

10

10

17

15

16

Fixed angle (25°)

4,000 g

87

50

9 ml

Min.

86

Rec.

97%

97%

98%

96%

95%

99%

98%

95%

94%

92%

* 0.25 mg/ml

** 1 mg/ml

*** 3,000 g + centrifugal speed

8 |

Chemical Compatibility

Table 4: Chemical Compatibility (2hr contact time)

Solutions PES

Compatible pH range pH 1–14

Acetic Acid (25.0%)

Acetone (10.0%)

OK

OK

Acetonitrile (10.0%)

Ammonium Hydroxide (5.0%)

OK

?

Ammonium Sulphate (saturated) OK

Benzene (100%) NO n-Butanol (70%)

Chloroform (1.0%)

OK

NO

Dimethyl Formamide (10.0%)

Dimethyl Sulfoxide (5.0%)

Ethanol (70.0%)

Ethyl Acetate (100%)

?

OK

OK

NO

Formaldehyde (30%)

Formic Acid (5.0%)

Glycerine (70%)

Guanidine HCI (6 M)

Hydrocarbons, aromatic

Hydrocarbons, chlorinated

Hydrochloric Acid (1 M)

Imidazole (500 mM)

Isopropanol (70%)

NO

NO

OK

OK

OK

OK

OK

OK

OK

Solutions PES

Compatible pH range pH 1–14

Lactic Acid (5.0%)

Mercaptoethanol (10 mM)

OK

OK

Methanol (60%)

Nitric Acid (10.0%)

OK

OK

Phenol (1.0%)

Phosphate Buffer (1.0 M)

Polyethylene Glycol (10%)

Pyridine (100%)

Sodium Carbonate (20%)

Sodium Deoxycholate (5.0%)

?

OK

Sodium Dodecylsulfate (0.1 M) OK

Sodium Hydroxide (2M) OK

?

OK

OK

?

Sodium Hypochlorite (200 ppm) ?

Sodium Nitrate (1.0%) OK

Sulfamic Acid (5.0%)

Tetrahydrofuran (5.0%)

OK

NO

Toluene (1.0%)

Trifluoroacetic Acid (10%)

Tween ®

Triton ®

20 (0.1%)

X-100 (0.1%)

Urea (8 M)

NO

OK

OK

OK

OK

OK = Acceptable ? = Questionable NO = Not recommended

| 9

Ordering Information

Vivaspin ® Turbo 4

Cut off

3,000 MWCO

3,000 MWCO

5,000 MWCO

5,000 MWCO

10,000 MWCO

10,000 MWCO

30,000 MWCO

30,000 MWCO

50,000 MWCO

50,000 MWCO

100,000 MWCO

100,000 MWCO

100

25

100

25

100

25

100

Qty per box

25

100

25

100

25

Vivaspin ® Turbo 15

Cut off

3,000 MWCO

3,000 MWCO

5,000 MWCO

5,000 MWCO

10,000 MWCO

10,000 MWCO

30,000 MWCO

30,000 MWCO

50,000 MWCO

50,000 MWCO

100,000 MWCO

100,000 MWCO

12

48

12

48

12

48

Qty per box

12

48

12

48

12

48

Prod. no.

VS04T91

VS04T92

VS04T11

VS04T12

VS04T01

VS04T02

VS04T21

VS04T22

VS04T31

VS04T32

VS04T41

VS04T42

Prod. no.

VS15T91

VS15T92

VS15T11

VS15T12

VS15T01

VS15T02

VS15T21

VS15T22

VS15T31

VS15T32

VS15T41

VS15T42

10 |

Other Products

Product

Vivaspin ® 500

Vivaspin ® 2

Sample volume

100 µl–600 µl

0.4 ml–2 ml

Centrisart

Vivaspin ® 6

Vivaspin ® 15R

Vivaspin ® 20

Vivacell 70

Vivacell 100

Vivacell 250

Vivaflow 50

0.5 ml–2.5 ml

2 ml–6 ml

2 ml–15 ml

5 ml–20 ml

10 ml–70 ml

20 ml–100 ml

50 ml–250 ml

100 ml–>5 l

Vivaflow 200 500 ml–>5 l

Vivaflow 50R

Vivapore 5

100 ml - 1 l

1 ml–5 ml

Vivapore 10/20 2 ml–10 ml/20 ml

Mode

Centrifugal

Centrifugal

Centrifugal

Centrifugal

Centrifugal

Centrifugal

Gas pressure

Centrifugal

Gas pressure

Centrifugal

Gas pressure

Gas pressure

Tangential flow

Tangential flow

Tangential flow

Solvent absorption

Solvent absorption

Membranes available

Polyethersulfone

Polyethersulfone,

Cellulose Triacetate,

Hydrosart ®

Polyethersulfone,

Cellulose Triacetate

Polyethersulfone

Hydrosart ®

Polyethersulfone

Polyethersulfone

Polyethersulfone

Polyethersulfone

Polyethersulfone,

Regenerated Cellulose

Polyethersulfone,

Hydrosart ®

Hydrosart ®

Polyethersulfone

Polyethersulfone

In Vitro Diagnostic Product Labeling

The following table defines the symbols found on Vivaspin ® Turbo 4 & 15 10K device labels.

Symbol Definition

In vitro diagnostic medical device

Catalogue number

Symbol Definition

Date of manufacture

Manufacturer

Do not reuse

Use by

Batch code C

Temperature limitation

Non-sterile product

CE conformity marking

| 11

Made in UK

Sartorius Stedim Lab Ltd.

Unit 6, Stonedale Road,

Stonehouse, Gloucestershire,

GL10 3RQ. UK www.sartorius-stedim.com

Copyright by Sartorius Stedim

Biotech GmbH, Goettingen,

Germany. All rights reserved.

No part of this publication may be reprinted or translated in any form or by any means without the prior written permission of

Sartorius Stedim Biotech GmbH.

The status of the information, specifications and illustrations in this manual is indicated by the date given below. Sartorius

Stedim Biotech GmbH reserves the right to make changes to the technology, features, specifications and design of the equipment without notice.

Status:

December 2015,

Sartorius Stedim Biotech GmbH,

Goettingen, Germany

Triton ® is a registered trademark of Union Carbide Corp.

Tween ® is a registered trademark of ICI Americas Inc.

Specifications subject to change without notice.

Copyright Sartorius Lab Instruments GmbH & Co. KG.

Printed in the EU on paper bleached without chlorine.

Vivaspin ® Turbo 4 and 15 · W

Publication No.: SLU6126-e151210

Ver. 12 | 2015