At Gilson “It is our policy to promote, with the leadership of our management, a total commitment to quality by each individual throughout our organization worldwide. It is our goal that this commitment to quality will result in complete customer satisfaction with both our products and services. For this purpose, we have established and implemented a formal quality system. Performance of our quality system is monitored to ensure that we are meeting our customers’ requirements and that we continually improve the effectiveness of the system and the progress in achievement of our goal.” Pipetman® Ultra 2-3 Pipetman® Ultra Multichannel & D300 4-5 Pipetman P & 8 X200 6-7 Pipetman F 8 Pipette Kits 9 Diamond® Tips 10-13 Microman® and Capillary Pistons 14-15 Distriman® Pipette & DistriTips 16 402 Dilutor-Dispenser 17 Gilson Pipetting Aid 18 Accessories 19 Minipuls 3 Peristaltic Pump 20-21 Customized Service 22-23 Pipette Replacement Parts 24-25 W! E N Gilson is pleased to announce new products for 2004: Pipetman Ultra Multichannel 4-5 Pipetman Ultra Multichannel is not only accurate and precise but also productive and comfortable at the same time. D300 Diamond Tips 4 Diamond D300 and Pipetman Ultra Multichannel is Gilson’s new multichannel pipetting system. D5000 and D10ml Tips 11 D5000 and D10ml are soon to be available racked in Tipacks. Gilson Pipetting Aid 18 For rapid and controlled pipetting. Pipetman Service Pack 23 Try out Gilson’s latest service offer with the new Pipetman Service Pack. ® PIPETMAN Ultra Advanced Pipetting at Your Fingertips Pipetman Ultra is the latest member of the Pipetman family, sharing many of the same characteristics, robustness, accuracy and precision with additional improvements and innovations. Ultra Comfort in Daily Use • Ergonomic design fits naturally in your hand and requires less effort to use. Less force is needed to set the volume, to pipette and to eject the tip, which makes for smoother pipetting on a daily basis. • Quick volume setting Only ten turns are needed to change from the minimum to the nominal volume setting. • Easy ejection & higher tip compatibility Adjustable tip-ejector stroke (patented) for maximum compatibility with tips and for improved comfort when ejecting tips. Fitted to all models including U5000 and U10ml (not adjustable). • Natural movement 3-position tip-ejector may be set for left or right-handed users, and less force is required to eject the tips. Ultra Easy Maintenance • Removable tip-ejector for easy access to the user- serviceable parts. Tip-ejector is made of PVDF to avoid corrosion. • Two-part tip holder for easy maintenance and cleaning. • Electronic adjustment is simple, only one tool is needed. OUR PIPETTES CONFORM TO 2 ISO8655 Ultra GLP and Security Features Ultra Accuracy and Precision • Lockable volume with on screen display for no risk of volume change during pipetting. • Pipetting workflow Exclusive patented multifunctional electronic display with greater readability and zero risk of parallax errors. control Two pipetting cycle counters included for quality control and servicing purposes. • Color-coded thumb wheel and push button to identify the model and the corresponding Diamond Tip range. Pipetman Ultra Models Model Diamond Tips* U2 Reference Number F21021 D10, DL10 U10 D10, DL10 U20 Min. Min. Max. F21023 Min. D200 Max. U100 F21024 D200 U200 Min. Max. F21025 Min. D200 Max. U1000 D10ml Random error (µL) Systematic error (µL) Random error (µL) 0.2 0.5 2 ±0.024 ±0.025 ±0.030 ≤0.012 ≤0.012 ≤0.014 ±0.08 ±0.08 ±0.08 ≤0.04 ≤0.04 ≤0.04 1 5 10 ±0.025 ±0.075 ±0.100 ≤0.012 ≤0.030 ≤0.040 ±0.12 ±0.12 ±0.12 ≤0.08 ≤0.08 ≤0.08 2 5 10 20 ±0.10 ±0.10 ±0.10 ±0.20 ≤0.03 ≤0.04 ≤0.05 ≤0.06 ±0.20 ±0.20 ±0.20 ±0.20 ≤0.10 ≤0.10 ≤0.10 ≤0.10 20 50 100 ±0.35 ±0.40 ±0.80 ≤0.10 ≤0.12 ≤0.15 ±0.80 ±0.80 ±0.80 ≤0.30 ≤0.30 ≤0.30 20 50 100 200 ±0.50 ±0.50 ±0.80 ±1.60 ≤0.20 ≤0.20 ≤0.25 ≤0.30 ±1.60 ±1.60 ±1.60 ±1.60 ≤0.60 ≤0.60 ≤0.60 ≤0.60 Min. 200 500 Max. 1000 ±3 ±4 ±8 ≤0.6 ≤1 ≤1.5 ±8 ±8 ±8 ≤3 ≤3 ≤3 F21027 Min. 1000 2000 Max. 5000 ±12 ±12 ±30 ≤3 ≤5 ≤8 ±40 ±40 ±40 ≤15 ≤15 ≤15 F21028 Min. ±30 ±30 ±40 ±60 ≤6 ≤6 ≤10 ≤16 ±60 ±60 ±60 ±60 ≤30 ≤30 ≤30 ≤30 D5000 U10ml Systematic error (µL) F21026 D1000 U5000 ISO 8655 Maximum Permissible Errors Volume (µL) Max. F21022 Gilson Maximum Permissible Errors 1mL 2mL 5mL Max. 10mL * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available. (See pages 10-13). More information available at www.gilson.com 3 W! NE ® PIPETMAN Ultra Higher Productivity Through Greater Comfort Whether you choose the 8 or 12 channel version, only two models of Pipetman Ultra Multichannel are needed to cover the entire volume range from 1 µL to 300 µL. Comfort • Designed to fit comfortably into anyone’s hand, whatever the size ! • Tipacks and TowerPack have our patented Rocky Rack™ feature making Diamond Tips easy to fit. GLP Note that the handle of Pipetman Ultra Multichannel shares the same design and GLP features as the single-channel version (see pages 2-3). Channel numbers are embossed on the head. W! NE DIAMOND Tips D300 • Green signifies 20 – 300 µL in the Diamond Tips range. • Designed to fit Pipetman Ultra Multichannel 8x300 and 12x300. • Large choice of packaging to fit your needs and your budget. (See page 12 for reference numbers) OUR PIPETTES CONFORM TO 4 ISO8655 Multichannel Greater Tip Compatibility Each model can be fitted with either of two tips • For 8x20 and 12x20 use DL10 tips. • For 8x300 and 12x300 use D300 tips. • D200 can be fitted to any model. The tip-ejector stroke may be adjusted to match the tips fitted. Pipetman Ultra Models Model Diamond Tips* Reference Number 8x20 12x20 DL10 D200 F21040 F21041 8x300 12x300 D200 D300 F21042 F21043 Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Systematic error (µL) Random error (µL) Systematic error (µL) Random error (µL) 1 2 10 20 ±0.10 ±0.10 ±0.20 ±0.40 ≤ 0.08 ≤ 0.08 ≤ 0.10 ≤ 0.20 ±0.40 ±0.40 ±0.40 ±0.40 ≤ 0.20 ≤ 0.20 ≤ 0.20 ≤ 0.20 20 30 150 300 ±1.00 ±1.05 ±1.25 ±3.00 ≤ 0.35 ≤ 0.53 ≤ 1.50 ≤ 3.00 ±8.00 ±8.00 ±8.00 ±8.00 ≤ 3.00 ≤ 3.00 ≤ 3.00 ≤ 3.00 Volume (µL) Min. Max. Min. Max. * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available (see pages 10-13). More information available at www.gilson.com 5 ® PIPETMAN P The Pipetting Standard Pipetman P is a fully adjustable, air-displacement pipette with the selected volume shown on a digital indicator (volumeter). Designed and manufactured to provide you with a range of robust, accurate and precise pipettes. Eight models cover the full range of volumes from 0.2 µL to 10 mL. The Standard for Robustness • Built to last, constructed of stainless-steel and PVDF (a plastic with very high-resistance to chemical attack). The Standard for Accuracy and Precision • Unique “dry seal” technology avoids using grease to obtain an adequate airtightness, and guarantees long-term performances. • Thick body (handle) in PVDF to minimize the hand-warming effect during pipetting. • Rigourous quality control - each piston is checked using a microscope. Easy Maintenance • Routine cleaning is all that is required to keep Pipetman in top condition. No lubrication is required. • Parts that may come in contact with liquids are easy to clean or replace. The tip-holder and tip-ejector are fully autoclavable. OUR PIPETTES CONFORM TO 6 ISO8655 • P2 and P10 equipped • P5000 and P10ml are safer with dual position tipejector, allowing the use of Diamond D10 (short) and DL10 (long) tips : a plus for protection and precision when using microtubes. and more accurate than glass pipettes. They are equipped with a polyethylene filter that prolongs piston life expectancy and prevents contamination. Pipetman P Models Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Systematic error (µL) Random error (µL) Systematic error (µL) Random error (µL) 0.2 0.5 2 ±0.024 ±0.025 ±0.030 ≤0.012 ≤0.012 ≤0.014 ±0.08 ±0.08 ±0.08 ≤0.04 ≤0.04 ≤0.04 1 5 10 ±0.025 ±0.075 ±0.100 ≤0.012 ≤0.030 ≤0.040 ±0.12 ±0.12 ±0.12 ≤0.08 ≤0.08 ≤0.08 2 5 10 20 ±0.10 ±0.10 ±0.10 ±0.20 ≤0.03 ≤0.04 ≤0.05 ≤0.06 ±0.20 ±0.20 ±0.20 ±0.20 ≤0.10 ≤0.10 ≤0.10 ≤0.10 20 50 100 ±0.35 ±0.40 ±0.80 ≤0.10 ≤0.12 ≤0.15 ±0.80 ±0.80 ±0.80 ≤0.30 ≤0.30 ≤0.30 50 100 200 ±0.50 ±0.80 ±1.60 ≤0.20 ≤0.25 ≤0.30 ±1.60 ±1.60 ±1.60 ≤0.60 ≤0.60 ≤0.60 Model Diamond Tips* Reference Number Volume (µL) P2 F144801 Min. D10 DL10 P10 Max. F144802 D10 DL10 P20 Min. Max. F123600 Min. D200 Max. P100 F1236154 D200 P200 Max. F123601 D200 P1000 F123602 Min. 200 500 Max. 1000 ±3 ±4 ±8 ≤0.6 ≤1 ≤1.5 ±8 ±8 ±8 ≤3 ≤3 ≤3 F123603 Min. 1000 2000 Max. 5000 ±12 ±12 ±30 ≤3 ≤5 ≤8 ±40 ±40 ±40 ≤15 ≤15 ≤15 F123201 Min. 1mL 2mL 5mL Max. 10mL ±30 ±30 ±40 ±60 ≤6 ≤6 ≤10 ≤16 ±60 ±60 ±60 ±60 ≤30 ≤30 ≤30 ≤30 F161004 Min. ±0.50 ±0.50 ±1.00 ±2.00 ≤0.25 ≤0.25 ≤0.50 ≤1.00 ±3.20 ±3.20 ±3.20 ±3.20 ≤1.20 ≤1.20 ≤1.20 ≤1.20 D5000 P10ml D10ml P8x200 Min. Max. D1000 P5000 Min. D200 Max. 20 50 100 200 * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available (see pages 10-13). More information available at www.gilson.com 7 ® PIPETMAN F Right Pipette ! Right Volume ! Right Price ! Pipetman F is a fixed-volume, air-displacement pipette. Thirteen robust models cover a large volume range from 2 µL to 1000 µL, with Pipetman legendary accuracy and precision. Right Pipette • GLP compliance - no risk of volume selection errors. Using a dedicated fixed-volume Gilson pipette for a specific test assures reliable and consistent results. Right Volume • Can be readjusted by users in the laboratory, for dense or viscous fluids (once reset the new volume cannot be changed accidentally). Right Price • An economical choice for clinical diagnostics, quality control, and any routine testing. Pipetman F Models Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Model Diamond Tips* Reference Number Volume (µL) Systematic error (µL) Random error (µL) Systematic error (µL) Random error (µL) F2 D200 F123770 2 ±0.08 ≤0.03 ±0.08 ≤0.04 F5 D200 F123771 5 ±0.10 ≤0.04 ±0.125 ≤0.075 F10 D200 F123772 10 ±0.10 ≤0.05 ±0.12 ≤0.08 F20 D200 F123774 20 ±0.20 ≤0.06 ±0.20 ≤0.10 F25 D200 F123775 25 ±0.25 ≤0.07 ±0.50 ≤0.20 F50 D200 F123778 50 ±0.40 ≤0.15 ±0.50 ≤0.20 F100 D200 F123784 100 ±0.80 ≤0.25 ±0.80 ≤0.30 F200 D200 F123605 200 ±1.60 ≤0.30 ±1.60 ≤0.60 F250 D1000 F123787 250 ±3.00 ≤0.75 ±4.00 ≤1.50 F300 D1000 F123788 300 ±3.50 ≤0.75 ±4.00 ≤1.50 F400 D1000 F123789 400 ±3.60 ≤0.80 ±4.00 ≤1.50 F500 D1000 F123790 500 ±4.00 ≤1.00 ±4.00 ≤1.50 F1000 D1000 F123606 1000 ±8.00 ≤1.30 ±8.00 ≤3.00 * Recommended tip for establishing maximum permissible errors (ISO 8655 requirement). A large range of suitable Gilson Diamond tips is available (see pages 10-13). OUR PIPETTES CONFORM TO 8 ISO8655 ® PIPETMAN Pipettes kits All You Need to Get Started, in a Single Box ! Our Pipette Kits now include the complete Pipetman® range for all of your pipetting needs in the laboratory. All in One • Each kit contains two or three classic Pipetman models plus samples of certified-quality Diamond Tips and useful accessories, such as Single Pipette Hangers (except Large Volume Kit). Economical Price • Save money when compared to pipettes bought separately. Pipetman Kits contents Model Reference Number Pipetman models Diamond Tips (racks of 96 tips) Accessories Pipetman Micro-Volume Kit F167500 P2 P10 P100 Sterilized FilterTips DFL10ST and DF100ST • 3 Single Pipette Hangers • 10 Coloris identification clips • 1 Gilson Guide to Pipetting Pipetman Starter Kit F167300 P20 P200 P1000 D200 and D1000 • 3 Single Pipette Hangers • 10 Coloris identification clips • 1 Gilson Guide to Pipetting Pipetman Large-Volume Kit F167400 P5000 P10ml 5 x D5000 tips 2 x D10ml tips (no racks) • 2 x 10 protection filters for P5000 and P10ml OUR PIPETTES CONFORM TO ISO8655 More information available at www.gilson.com 9 ® DIAMOND Tips High Quality Tips for High Quality Pipettes Quality Tips • Gilson Logo • Traceability guarantees quality according to ISO 8655 requirements, Diamond Tips and Pipetman pipettes are the complete pipetting system. From the mold to the bench, the batch number is traceable. 10 µl 300 µl 200 µl 1000 µl 30 µl BATCH No. Sterilize filter tip d DF30 s ST BA BATCH No. TCH N o. FL89/0 BATCH No. 0057 • Level marks BATCH No. Ster. da te : JAN 99 These marks mean that a quick glance at the tip is all that is needed to check quickly the volume. For DL10: 2 µL and 10 µL. For D200: 20 µL and 100 µL. For D1000: 300 µL. • Totally autoclavable All the range of Diamond Tips can be autoclaved (121°C, 0.1MPa, 20 min), even filter tips. • Pure polypropylene • No dyes, no additives. Just translucent. Avoids liquid retention. Fine point and flexibility The last droplet is easier to dispense. • Perfect Air tightness As Gilson manufactures both Pipetman and Diamond Tips, the diameters of the tipcollar and tip holder are calculated to fit perfectly. OUR PIPETTES CONFORM TO 10 ISO8655 • Color coded for easy identification Diamond packaging and labels are color coded for volume recognition at a glance. Just place the Tower pack over the empty box 1 DIAMOND Tips Tower Pack™ Refill System Easy, simple, practical, and available sterilized 2 Press down 96 tips snap into place 3 • The red sticker guarantees sterilization. • The same universal reload box for all Diamond Tips simplifies your work. W! NE D5000 and D10ml in TIPACK™ Racks* • D5000 & D10ml have been designed to fit perfectly with Pipetman Ultra U5000 & U10ml (See pages 2 & 3). • Tips are easy to pick-up D10ml Tipack of 30 tips D5000 Tipack of 50 tips directly from the rack. Item Reference Number Tipack D5000 (12 Tipacks of 50 tips) F161370 Tipack D10ml (8 Tipacks of 30 tips) F161230 * Available soon ! More information available at www.gilson.com 11 ® DIAMOND Tips Large Choice of Packaging to Fit Your Activity and Budget Color coding Pipette models Volume range Tipack Dia 10 Tipacks of Autoclavable D10 P2 P10 U2 U10 0.2 - 10 µL F161632 DL10 P2 P10 U2 U10 8x20 12x20 0.2 - 20 µL F161452 D200 P20 to P200 U20 to U200 8x200 8x20 12x20 8x300 12x300 2 - 200 µL F161932 D300 8x300 12x300 20 - 300 µL F161732 D1000 P1000 U1000 200 - 1000 µL F161672 D5000 P5000 U5000 1 - 5 mL F161370 (12 Tipacks of 50 tips) D10ml P10ml U10ml 1 - 10 mL F161230 (8 Tipacks of 30 tips) Work safety with the Diamond Filter Tips DF10 DFL10 DF30 DF100 DF200 DF300 DF1000 OUR PIPETTES CONFORM TO 12 ISO8655 P2 P10 U2 U10 P2 P10 U2 U10 8x20 12x20 P20 U20 8x20 12x20 P100 U100 P200 U200 8x200 P200 U200 8x300 12x300 P1000 U1000 0.2 - 10 µL F161710 0.2 - 10 µL F161454 2 - 30 µL F161935 20 - 100 µL F161939 20 - 200 µL F161936 20 - 300 µL F161734 200 - 1000 µL F161770 mond Tips: 96 racked tips Tower Pack: refill system for Steril-Pack: Diamond Tips 10 racks of 96 tips 400 tips individually γ-Ray sterilized Autoclavable γ-Ray sterilized wrapped EcoPack: box of 10,000 tips EasyPack: box of 1,000 tips (10 bags of 1,000) (5 bags of 200) Empty Racks (set of 2) F161905 F167101 F167201 F161310 F161630 F161631 F161791 F161455 F167102 F167202 F161320 F161450 F161451 F161393 F161937 F167103 F167203 F161330 F161930 F161931 F161391 F161735 F167105 F167205 - F161730 F161731 F161737 F167104 F167204 F161340 F161670 F161671 F161793 (7 racks of 96 tips) (7 racks of 96 tips) - - F123969 F161360 F161975 - - F167104 - Item Reference Number Universal reload box F167100 - - (1 rack) - - F161210 F161220 (200 tips) (1 rack) No Contamination F161633 F161453 100 µl F161933 Sterilized filter tips DF100ST F161938 BATCH No. FL89/00057 F161934 • Diamond Filter Tips are autoclavable. F161733 F161673 • Available free of detectable RNase, DNase, DNA, RNA and protease. • The date of sterilization Ster. date : June 03 and the red sticker guarantee sterilization. • All sterilized Diamond Tip packagings are wrapped in medical device quality plastic, which provides a complete shield against contamination. More information available at www.gilson.com 13 MICROMAN Your Partner for the Pipetman Pipette Positive Displacement Pipette The desired volume is aspirated rapidly and completely No air-to-liquid interface The positive wiping action of the piston against the capillary wall assures accurate dispensing of even the most viscous sample and avoids any carry over Proven Accuracy and Precision • Can pipette all kinds of liquid with a high degree of accuracy and precision. • Large volume range from 1µL to 1000 µL. • Capillary Pistons (CPs) are precision molded from chemical resistant, non wetting polypropylene. • No need for calibration when you change the capillary - piston. Complete and rapid pipetting of viscous, dense, volatile or above temperature samples Positive displacement pipette no viscous liquid remains in the capillary-piston. The best choice for PCR and Biological samples Air displacement pipette viscous liquids adhere to the inside wall of the tip. Safest Pipette Contamination Free Pipetting • Prevents aerosol formation. • Protects against residual sample carry-over. • Unbreakable plastic capillary and piston. • Built-in tip ejector, no need to touch Non protected air space: risk of aerosol contamination Protected air space, no aerosol contamination no vapors the disposable CP. • Capillary Pistons are certified free of detectable RNase, DNase, RNA, DNA and protease. OUR PIPETTES CONFORM TO 14 ISO8655 Regular Air Displacement Pipette Microman Positive Displacement Pipette ® MICROMAN and Capillary Pistons LIQUID APPLICATIONS Microman Models Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Systematic error (µL) Random error (µL) Systematic error (µL) Random error (µL) 1 5 10 ±0.06 ±0.10 ±0.15 ≤0.02 ≤0.03 ≤0.05 ±0.20 ±0.20 ±0.20 ≤0.10 ≤0.10 ≤0.10 3 10 25 ±0.15 ±0.17 ±0.25 ≤0.06 ≤0.08 ≤0.10 ±0.70 ±0.70 ±0.70 ≤0.30 ≤0.30 ≤0.30 20 50 ±0.34 ±0.50 ≤0.12 ≤0.15 ±0.70 ±0.70 ≤0.30 ≤0.30 10 50 100 ±0.50 ±0.75 ±1.00 ≤0.20 ≤0.30 ≤0.40 ±1.50 ±1.50 ±1.50 ≤0.60 ≤0.60 ≤0.60 50 100 250 ±1.50 ±1.70 ±2.50 ≤0.20 ≤0.30 ≤0.50 ±6.00 ±6.00 ±6.00 ≤2.00 ≤2.00 ≤2.00 200 500 Max. 1000 ±3.00 ±5.00 ±8.00 ≤1.60 ≤2.50 ≤4.00 ±12.00 ±12.00 ±12.00 ≤4.00 ≤4.00 ≤4.00 Model Reference (Capillary Piston) Number Volume (µL) M10 (CP10) Min. F148302 Max. M25 (CP25) F148002 M50 (CP50) F148003 Min. Max. Min. Max. M100 (CP100) F148304 Min. Max. M250 (CP250) F148004 Min. Max. M1000 (CP1000) F148005 Min. • Whole blood • Syrup • Oil • Glycerol • Solvents, alcohol • Cosmetic cream • Mercury • Nitric acid 65% • Tetrahydrofuran • DNA fragments • PCR templates • Enzyme solutions • Radio-labelled compounds Microman Capillary Pistons Reference number Capillary Piston Microman Model Quantity/Box Sterilized Assembled Racked in Tipack F148412 CP10 M10 192 No Yes Yes F148312 CP10 M10 960 No Yes Yes F148413 CP10ST M10 192 Yes Yes Yes F148313 CP10ST M10 960 Yes Yes Yes F148112 CP25 M25 200 No No No F148113 CP50 M50 200 No No No F148414 CP100 M100 192 No Yes Yes F148314 CP100 M100 960 No Yes Yes F148415 CP100ST M100 192 Yes Yes Yes F148315 CP100ST M100 960 Yes Yes Yes F148114 CP250 M250 200 No No No F148145 CP1000 M1000 200 No Yes No F148180 CP1000ST M1000 182 Yes Yes Yes More information available at www.gilson.com 15 ® DISTRIMAN andSimplify DistriTips Repetitive Pipetting Distriman is a positive-displacement, continuously adjustable repeater-pipette to simplify multiple dispensing when used with Gilson pure-polypropylene DistriTip syringes. Direct Volume Readout • With the Distriman you can dispense the exact volume, eliminating tedious calculations. Only 3 Syringes Are Needed • Micro 125 µL, Mini 1250 µL and Maxi 12.5 mL cover the entire volume range (from 1 µL to 1.25 mL). DistriTips are the only syringes capable of dispensing at least 100 aliquots. Wide Selection of Packaging Options • Each model of DistriTip is available in standard or sterilized (ST) version, in boxes of 50 syringes. Sterilized DistriTips are individually wrapped and certified free of RNase, DNase, DNA, RNA and protease. Distriman Reference Number F164001 Gilson Maximum Permissible Errors ISO 8655 Maximum Permissible Errors Systematic error (µL) Random error (µL) Systematic error (µL) Random error (µL) 2 5 10 ±0.100 ±0.125 ±0.200 ≤ 0.080 ≤ 0.075 ≤ 0.100 ±0.20 ±0.20 ±0.20 ≤ 0.10 ≤ 0.10 ≤ 0.10 20 50 100 ±0.80 ±1.00 ±1.00 ≤ 0.20 ≤ 0.40 ≤ 0.60 ±1.50 ±1.50 ±1.50 ≤ 0.60 ≤ 0.60 ≤ 0.60 200 500 Max. 1000 ±6.00 ±7.50 ±10.0 ≤ 1.00 ≤ 1.50 ≤ 2.50 ±12.00 ±12.00 ±12.00 ≤ 4.00 ≤ 4.00 ≤ 4.00 DistriTips (box of 50 syringes) Model Reference Number 125 µL - 1 µL to 12.5 µL Micro F164100 Micro ST F164130 Volume (µL) Min. Max. 1250 µL - 10 µL to 1250 µL Mini F164110 Mini ST F164140 Min. 12.5 mL - 100 µL to 1.25 mL Maxi F164120 Maxi ST F164150 Min. Max. ST means sterilized syringes (individually wrapped). OUR PIPETTES CONFORM TO 16 ISO8655 ® 402 Dilutor-Dispenser Liquid Handling at the Touch of a Button Increase productivity with Gilson’s 402 Dilutor Dispenser, because it’s faster than any other pipettor for repetitive liquid transfer and is fully programmable (save and recall up to 99 methods). GLP Compliance • • • • Eliminates technician-to-technician variability. Calibration certificate, IQ/OQ procedure. Coded protection of methods. LED indicator on the probe for error free manipulation. Comfortable • Just push the button -same comfort as a motorized pipette. With a light weight probe, less than 50g. Weight (g) • Comparative weights 200 150 100 50 0 Outperforms the Competition • • 402 DD probe mechanical motorized pipette pipette Handles volumes from 1 µL to 99 mL. Compensation of the liquid temperature and viscosity. 9 Prestored Tasks • Dilute, dilute serially, dispense (aliquots or variable volumes), pipette, rinse, mix, titrate, measure unknown volume and prime. Available Models (without syringes, which are sold separately) Reference Number 402 Dilutor-Dispenser, single-syringe, 110-220V F410514 402 Dilutor-Dispenser, single-syringe, 220V (French version) F410514F 402 Dilutor-Dispenser, dual-syringe, 110-220V F410515 402 Dilutor-Dispenser, dual-syringe, 220V (French version) F410515F Additionnal Accessories No contamination : the probe may be fitted with Diamond Tips Reference Number Adapter Kit for disposable tips F4015015 Syringe, 100 µL 25025341 Syringe, 250 µL 25025342 Syringe, 500 µL 25025347 Syringe, 1 mL 25025343 Syringe, 5 mL 25025344 Syringe, 10 mL 25025345 Syringe, 25 mL 25025346 OUR PIPETTES CONFORM TO ISO8655 More information available at www.gilson.com 17 W! NE Gilson Pipetting Aid Rapid and Controlled Pipetting with Precision and Safety Comfortable • Ergonomic design for increased user comfort. • Motorized operation. • Precise and smooth finger tip control of variable speed range. “Wings” to enable you to rest it on the bench. High Performance • Powerful battery, continuous work for 8 hours (Environmentally friendly NiMH). • Works with all standard pipettes. • Fills 25 mL pipette in 4 seconds. • Selectable operation modes according to volume and viscosity. Safe to Use • Safety valve and filter prevent contamination. • All parts in contact with liquid are autoclavable. • CE certified. Description Tip adaptor for D5000 and D10ml with filter. Sterile and economical solution to handle liquid volumes from 1 mL to 10 mL Reference Number Gilson Pipetting Aid (220V/50Hz) with European standard plug F161250 Gilson Pipetting Aid (220V/50Hz) without power Supply F161240 Charging stand F161288 Adaptor for D5000 and D10ml Diamond Tips F161287 Pipette holder F161256 Nose-piece F161249 Nose-piece hanger F161253 PTFE autaclavable filter 0.2 µm (5 units) F161245 PTFE autaclavable filter 0.45 µm (5 units) F161246 If you want to order a Gilson Pipetting Aid with a US, UK, or other a country’s power supply, please contact your local Gilson distributor. OUR PIPETTES CONFORM TO 18 ISO8655 Charging and storage stand. The Gilson Pipetting Aid is always fully charged and ready to use. Pipette Accessories Quality Accessories for Quality Pipettes Gilson accessories are of the same high quality as Gilson pipettes. Gilson considers accessories as a part of the pipetting system. All pipettes should be stored vertically to avoid the possibility of liquid running back into, and contaminating or corroding, the interior of the pipette. Carrousel™ Pipette Stand • A convenient, rotating stand–holds up to seven Gilson pipettes (any model) with exceptional stability. Protects your precious pipettes from possible damage or contamination. Trio™ Pipette Stand • Trio is a portable pipette stand that keeps your Gilson pipettes within easy reach. A great accessory for use in fume hood. Single™ Pipette Holder* • An adhesive-backed holder, designed for use where space is limited (fume hoods, sterile hoods, etc.). Sticks to any smooth, dry surface without damage. Resistant to chemicals and UV light. Coloris™ Identification Clips • GLP friendly, new brightly colored clips are used to identify or personalize your Pipetman pipette. Made of polyacetal, Coloris clips accept permanent ink - they just snap into place. Pipetman® Ultra Name Tags • Personalize your Pipetman Ultra by writing your name or application on one of the color coded stickers (protected by the plastic window). * Suitable for all Gilson pipettes except for the Pipetman Ultra range. New single holder will soon be available for all Gilson pipettes. Description Carrousel pipette stand, for 7 Gilson pipettes Reference Number F161401 Trio pipette stand, for 3 Gilson pipettes F161405 Single pipette holder F161402 Coloris Clips, bag of 10 (mixed colors) F161301 Coloris Clips, bag of 10 (mixed colors) F161302 Coloris Clips, bag of 10 (mixed colors) F161303 Coloris Clips, bag of 10 (mixed colors) F161304 Coloris Clips, bag of 10 (mixed colors) F161305 Coloris Clips, bag of 10 (mixed colors) F161306 Pipetman Ultra Name Tags (set of 5 colors) F2070901 OUR PIPETTES CONFORM TO ISO8655 More information available at www.gilson.com 19 MINIPULS 3 Gilson's Reliable Solution for Laboratories High Performance and Versatile • Pulse-free flow (10 stainless steel rollers!). • Excellent flow-rate accuracy and reproducibility. • Wide flow-rate range from 0.3 µL/min to 220 mL/min. • Wide tubing-size range from 0.25mm to 8mm. • Operates at higher pressures than competitor products (up to 0.5 MPa). • Low noise operation. Reliable and Robust • High chemical resistance material (drive module and pump heads). • Motor speed stability (high torque stepper motor). • High quality tubing for better reproducibility and longer life. • Built-in overflow system, in case of tubing leakage. Easy to Use • Ready to use, can be installed within minutes. • Digital keypad. • Quick change of pump head. • Easy to integrate into your system. Complete information on tubing materials and flow rates is available from your local distributor and to be found on the Gilson web-site. OUR PIPETTES CONFORM TO 20 ISO8655 The key to a successful peristaltic-pump application is to optimize the selection of the pump-head, tubing and drive. In addition to an outstanding peristaltic pump, Gilson supplies high quality tubings and connectors. Peristaltic Pump Adjustable cams allow fine flow-rate adjustment to minimize tubing wear Two-stop calibrated tubing makes it easy to install tubing on the pump head. Tubing Material PVC Silicone Pharmed Isoversinic (Viton) Composition Polyvinyl chloride Silicone elastomer Polypropylenebased material Fluorinated elastomer Physical characteristics Transparent clear Transluscent beige Opaque beige Opaque black Temperature range Up to 94°C Up to 230°C -60 to 135°C -20 to 200°C Standards FDA food quality (FDA 21CFR 177.26000) USP class VI Gas permeability Low High Low Impermeable to most gases Sterilization Autoclaving Autoclaving or irradiation Autoclaving Autoclaving Range of internal diameter 0.25 to 8 mm 0.6 to 7 mm 0.5 to 6.4 mm 0.5 to 6.0 mm Other Low cost Excellent biocompatibility Long tubing life Excellent resistance to strong acids, oxidizing agents General use Biological Pharmaceutical and food Where high chemical resistance is required features Applications Pharmed is a Trademark of Norton Performance Plastics Corp., Akron, OH, USA. Maximum flow rate depending on the pump head and tubing (complete table available on Gilson web site). Approx. values: determined with water (grade 3 ISO 3696) at 21.5°C, no differential pressure. The information in the table related to tubing materials has been supplied to Gilson by the manufacturers and is for guidance only - Gilson recommends that you test the tubing before use. Item Max Flow Rate Number of channel Speed Control Module Reference Number F155001 R1 Pump Head up to 30 mL/min 1 F117604 R2 Pump Head up to 30 mL/min 2 F117800 R4 Pump Head up to 30 mL/min 4 F117606 R8 Pump Head up to 30 mL/min 8 F117608 R2/HF Pump Head (High Flow) up to 220 mL/min 2 F117830 R4/HF Pump Head (High Flow) up to 220 mL/min 4 F117831 More information available at www.gilson.com 21 Customized Service At Gilson, we believe that the quality of our pipettes must be matched by the quality of our service. That is why we have a network of authorized service centers in more than 50 countries around the world. These repair and calibration centers duplicate the conditions at our own factory, with its controlled environment and regular inspections. The service centers are regularly tested by Gilson to verify their servicing skills. Qualified technicians at these centers, having completed extensive training by Gilson, are specialists in the repair, calibration, and operation of our products according to ISO 8655 standard and Good Laboratory Practice (GLP) requirements. To support your standard operating procedures (SOPs), Gilson also offers a range of technical documents covering performance checking and decontamination process of your pipette (contact your local Gilson distributor or refer to the Gilson website). Gilson Service Centers You may send your pipettes to the nearest Gilson Service Center – Contact your local Gilson representative for information. Service provided includes: • Diagnosis, repairs, and calibration – according to the tariff applicable at the Gilson Service Center • Preventive maintenance contract to establish an agreement with the Gilson Service Center to implement the level of service most appropriate to your needs (what, how often, number of pipettes, cycle time, etc.) • Calibration by gravimetric methods to check the performance of your pipettes according to ISO 8655 standard. Our goal is to ensure that all of your serviced Gilson Pipettes are as good as new! OUR PIPETTES CONFORM TO 22 ISO8655 On-Site Service Gilson’s qualified technicians are available to visit your facility for any of the following : • Diagnosis: Check that your Gilson pipettes function correctly and are mechanically sound. • Repairs: Replacement of basic or complex components. • Updating Pipetman: Systematic replacement of key components with upgraded parts. • Pipette Clinics: Servicing of all Gilson pipettes. Servicing may include cleaning, decontamination, repair, (adjustment if needed) and calibration. Contact your nearest Gilson Service Center for availability of these offer and details. Training Gilson conducts comprehensive seminars to “train the trainers”. This gives your local representative the knowledge to provide you with the best service possible on the use, maintenance, and calibration of your Gilson pipettes. He can offer you extensive pipette training, covering topics such as: • Use and applications. • GLP requirements, quality control, applicable. standards, ensuring traceability, and keeping records • Preventive maintenance and calibration (where appropriate). W! NE Pipetman Service Pack* No paperwork, no quotations! Just return the coupon with your pipette to your local Gilson Distributor to maintain your pipette performance for two years at an economical price. In every Pipetman Service Pack, you get the pipetting standard along with Gilson’s service expertise for two years. Each Service Pack contains: • Pipetman, accessories, and User’s Guide. • Coupons for FREE service in the first year after purchase and one halfprice service in the second year. • Warranty is extended to two years from the date of purchase. • Gilson assures services through your local distributor. *Not available in all countries. Please contact your local Gilson distributor for more information. Performance Checking by Yourself: Weighing Kit The weighing kit is intended for endusers to use to check the performance of their pipettes. It consists of four containers (of different sizes), filters, and tweezers, suitable for the gravimetric method. Item Reference Number Pipetman Service Pack P2 F144803 Pipetman Service Pack P10 F144804 Pipetman Service Pack P20 F123810 Pipetman Service Pack P100 F123825 Pipetman Service Pack P200 F123611 Pipetman Service Pack P1000 F123612 Pipetman Service Pack P5000 F123613 Pipetman Service Pack P10ml F161203 Weighing Kit F144700 QualiTrace F147608 QualiTrace™: Quality Management Software for Pipettes QualiTrace is a complete calibration and quality management package for GLP laboratories. The software is compatible with all Gilson pipettes. • Software is easy to configure for gravimetric calibration and is compatible with any balance. Use the standard method or enter your own parameters (number of weighings, volumes, temperature, pressure, etc.). • QualiTrace automatically manages the calibration protocol with direct transfer of the measurements from the balance to the software, which: establishes the calibration schedule, guides the user through the weighing process, calculates accuracy and precision, accepts or rejects the pipette, and prints a calibration report. • The audit trail features record entries by the user and any changes in key setup options. Complete device history is conserved intact. More information available at www.gilson.com 23 PIPETTE Replacement Parts Gilson replacement parts are validated according to a strict quality control program. They are engraved with the Gilson logo to give you the assurance that your pipette is repaired with genuine spare parts, ensuring top-level performance and trouble-free pipetting. Gilson spare parts are only available from your local, authorized Gilson representative. Pipetman Ultra Single-channel and Multichannel Replacement Parts E1 + E2 B D C F2 G F1 P U } A O H1 + H2 K M S Q R Pipetman Ultra Replacement parts Item Push-Button Assembly Spring guide Seal for U2 & U10 (or O-ring for other models) Piston Return Spring for piston Tip Holder, upper part Tip Holder, lower part Tip Ejector Assembly Tip Ejector extension for U2 & U10 Connecting Nut*** Battery Holder*** Battery, flat 1.5 V*** Recalibration tool*** Battery Compartment Cover*** Plastic window*** Lubricant Tube 2.5g*** key A B D E1 E2 F1 F2 G ** C H1 H2 K M N ** Pipetman Ultra Multichannel Replacement parts Item key Push-Button Assembly O Cover Ejector P Piston Assembly Q Airtighness Kit R Tip-Holder Assembly S Reassembly tool ** Wire clip U OUR PIPETTES CONFORM TO 24 ISO8655 U2 F2070152 F2070103 F161901 F2070160 F2070113 F2070117 F2070118 F2070159 F2070903 F2072111 F2070158 F20710 F20751 F2072107 F2072102 * U10 F2070252 F2070103 F161902 F2070260 F2070113 F2070117 F2070218 F2070159 F2070903 F2072111 F2070158 F20710 F20751 F2072107 F2072102 * Reference Number U20 U100 U200 F2070352 F2070452 F2070552 F2070103 F2070103 F2070103 F161903 F2070401 F2070501 F2070360 F2070460 F2070560 F2070113 F2070113 F2070113 F2070117 F2070417 F2070517 F2070318 F2070418 F2070518 F2070359 F2070459 F2070559 * * * F2072111 F2072111 F2072111 F2070158 F2070158 F2070158 F20710 F20710 F20710 F20751 F20751 F20751 F2072107 F2072107 F2072107 F2072102 F2072102 F2072102 F2070902 F2070902 F2070902 Reference Number 8X20 12X20 8X300 12X300 F2073052 F2073152 F2073252 F2073352 F2073059 F2073159 F2073259 F2073359 F2073060 F2073060 F2073260 F2073260 F2073065 F2073065 F2073265 F2073265 F2073063 F2073063 F2073263 F2073263 F2073001 F2073001 F2073201 F2073201 F207306407 F207306407 F207306407 F207306407 U1000 F2070652 F2070103 F2070601 F2070660 F2070113 F2070617 F2070618 F2070659 * F2072111 F2070158 F20710 F20751 F2072107 F2072102 F2070902 U5000 F2070752 * F2070701 F2070760 * F2070718 * F2070759 * * F2070158 F20710 F20751 F2072107 F2072102 F2070902 U10ml F2070852 * F2070801 F2070860 * F2070818 * F2070859 * * F2070158 F20710 F20751 F2072107 F2072102 F2070902 * Not applicable with this model. ** Not shown *** Suitable for Pipetman Ultra Multichannel Pipetman P Replacement Parts Pipetman P Replacement parts Item key Push-Button Assembly, volume setting A Seal & O-ring (set of 5) S1 + S2 Tip Holder H Connecting Nut C Adapter for Dual-position Tip Ejector (plastic part only, set of 5) ** Tip Ejector Assembly for P2 & P10 ** Tip Ejector Assembly E * Not applicable with this model. **Not shown P2 F144781 F144861 F144816 F123654 F144879 F144876 * Reference Number P20 P100 P200 F144783 F144784 F144785 F144863 F144864 F144865 F123353 F144602 F123305 F123654 F123654 F123654 * * * * * * F123657 F144605 F123658 P10 F144782 F144862 F144819 F123654 F144879 F144876 * P1000 F144786 F144866 F123371 F123654 * * F123659 P5000 F144787 F144867 F123608 * * * * P10ml F161281 F161829 F161263 * * * * S1 + S2 H A C E Pipetman F, 8X200 and Distriman Replacement Parts Pipetman F Replacement parts Item Piston Assembly Seal & O-ring (set of 5) Connecting Nut Tip Ejector Tip Holder Calibration key Refer to the User's Guide (LT801118) for details Reference Number F25 to F100 F200 F144611 F123846 F144864 F144865 F144723 F144723 F144767 F144768 F144602 F123305 F123674 F123674 F2 to F20 F123845 F144863 F144723 F144766 F123353 F123674 Pipetman 8X200 Replacement parts Item Reference Number Push-Button Assembly F1861081 Nozzle/Piston Assembly F161067 Silicon Grease F1485012 Spring (internal) F1861072 Cover (plus screws) F1861073 Refer to the User's Guide (LT801236) for details F250 to F1000 F123847 F144866 F144723 F144769 F123371 F123674 Distriman Replacement parts Item Reference Number Push-Button Assembly F164040 Piston Retaining Assembly F164070 Red flag (set of 5) F164042 Retaining Spring F1864052 Refer to the User's Guide (LT801285) for details Maintenance Kits for Pipetman P and F These include: piston seals, O-rings, and one tip-holder. Maintenance Kits Model P2 Reference Number F144501 P10 F144502 P20 F2 to F20 F144495 P100 F25 to F100 F144496 P200 F200 F144497 P1000 F250 to F1000 F144498 P5000 P10ml F144499 F144503 More information available at www.gilson.com 25 Gilson, Inc. World Headquarters 3000 W. Beltline Hwy., P.O. Box 620027, Middleton, WI 53562-0027 USA Telephone: (1) 800-445-7661 or (1) 608-836-1551 Fax: (1) 608-831-4451 www.gilson.com email@example.com, firstname.lastname@example.org, email@example.com Gilson S.A.S. 19, avenue des Entrepreneurs, BP 145, F-95400 Villiers-le-Bel, France Telephone: (33-1) 34 29 50 00 Fax: (33-1) 34 29 50 20 LT800451E. Printed in France, January 2004. Specifications subject to change without notice. Gilson, Inc. and Gilson S.A.S. are ISO 9001 certified.