MEA-System_Manual

(USB-) MEA-Systems Manual
Information in this document is subject to change without notice.
No part of this document may be reproduced or transmitted without the express written
permission of Multi Channel Systems MCS GmbH.
While every precaution has been taken in the preparation of this document, the publisher and
the author assume no responsibility for errors or omissions, or for damages resulting from the use
of information contained in this document or from the use of programs and source code that may
accompany it. In no event shall the publisher and the author be liable for any loss of profit or any
other commercial damage caused or alleged to have been caused directly or indirectly by this
document.
© 2013 Multi Channel Systems MCS GmbH. All rights reserved.
Printed: 05. 08. 2013
Multi Channel Systems
MCS GmbH
Aspenhaustraße 21
72770 Reutlingen
Germany
Fon
+49-71 21-90 92 5 - 0
Fax
+49-71 21-90 92 5 -11
info@multichannelsystems.com
www.multichannelsystems.com
Microsoft and Windows are registered trademarks of Microsoft Corporation. Products that
are referred to in this document may be either trademarks and/or registered trademarks of
their respective holders and should be noted as such. The publisher and the author make
no claim to these trademarks.
Table of Contents
1.1
Operator's Obligations
5
1.2
Guarantee and Liability
5
1.3
Important Safety Advice
6
2.1
Basic Components of (USB-) MEA-Systems
7
2.2
System Configurations and optional Components
8
2.2.1 Single Components for MEA-Systems
8
2.2.2 MEA2100-System
9
2.2.3 USB-MEA256-System
9
2.2.4 USB-MEA-Systems
9
2.2.5 MEA1060-BC Amplifier with Blanking Circuit
10
2.2.6 Enhanced Perfusion System (- E)
10
2.3
Accessories
10
2.4
Add-ons for Stimulation
11
2.5
Setting up the (USB-) MEA-System
12
3.1
Introduction
13
3.2
Background
13
3.3
Signal Types
14
3.3.1 Single Unit Activities
14
3.3.2 Local Field Potentials
14
3.4
14
Recording and Stimulation
3.4.1 Microelectrode Arrays (MEAs)
15
3.4.2 Signal Amplification and Filters
15
3.4.3 Data Acquisition
16
3.5
Using the additional Analog Inputs
18
3.6
Digital Input / Output, System Synchronization
19
3.6.1 Triggering the Data Acquisition and MC_Rack
19
3.6.2 Triggering other Instruments by MEA2100 or USB-Device19
4.1
About Troubleshooting
20
4.2
Triggering / Digital Input does not Work
20
4.3
Artifacts caused by Perfusion
21
5.1
Contact Information
22
5.2
Ordering Information
23
5.2.1 MEA2100-Systems
23
5.2.2 USB-MEA-Systems
25
5.2.3 Stimulus Generators
28
5.2.4 MEA Amplifiers
28
5.2.5 Accessories
28
Appendix
1
Important Information and Instructions
1.1
Operator's Obligations
The operator is obliged to allow only persons to work on the device, who

are familiar with the safety at work and accident prevention regulations and have been
instructed how to use the device;

are professionally qualified or have specialist knowledge and training and have received
instruction in the use of the device;

have read and understood the chapter on safety and the warning instructions in this manual
and confirmed this with their signature.
It must be monitored at regular intervals that the operating personnel are working safely.
Personnel still undergoing training may only work on the device under the supervision
of an experienced person.
1.2
Guarantee and Liability
The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply.
The operator will receive these no later than on conclusion of the contract.
Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests
and data generated by the use of the device or the software. It is up to the user to use good
laboratory practice to establish the validity of his findings.
Guarantee and liability claims in the event of injury or material damage are excluded when
they are the result of one of the following.

Improper use of the device.

Improper installation, commissioning, operation or maintenance of the device.

Operating the device when the safety and protective devices are defective and/or inoperable.

Non-observance of the instructions in the manual with regard to transport, storage, installation,
commissioning, operation or maintenance of the device.

Unauthorized structural alterations to the device.

Unauthorized modifications to the system settings.

Inadequate monitoring of device components subject to wear.

Improperly executed and unauthorized repairs.

Unauthorized opening of the device or its components.

Catastrophic events due to the effect of foreign bodies or acts of God.
5
1.3
Important Safety Advice
Warning: MEA-Systems include several instruments as individual components. Each instrument
is shipped with a separate Manual. The information in the individual Manuals fully apply to the
complete-System. This manual is only to be understood as an additional information. Read all
Manuals thoroughly before setting up the system.
Warning: Obey always the rules of local regulations and laws. Only qualified personnel should
be allowed to perform laboratory work. Work according to good laboratory practice to obtain
best results and to minimize risks.
The product has been built to the state of the art and in accordance with recognized safety
engineering rules. The device may only

be used for its intended purpose;

be used when in a perfect condition.

Improper use could lead to serious, even fatal injuries to the user or third parties and damage
to the device itself or other material damage.
Warning: The devices and the software are not intended for medical uses and must not
be used on humans.
Malfunctions which could impair safety should be rectified immediately.
High Voltage
Electrical cords must be properly laid and installed. The length and quality of the cords must
be in accordance with local provisions.
Only qualified technicians may work on the electrical system. It is essential that the accident
prevention regulations and those of the employers' liability associations are observed.

Each time before starting up, make sure that the mains supply agrees with the specifications
of the products.

Check the power cords for damage each time the site is changed. Damaged power cords should
be replaced immediately and may never be reused.

Check the leads for damage. Damaged leads should be replaced immediately and may never
be reused.

Do not try to insert anything sharp or metallic into the vents or the case of the products.

Liquids may cause short circuits or other damage. Keep the devices and the power cords always
dry. Do not handle it with wet hands.
Appendix
2
Welcome to (USB-) MEA-Systems
2.1
Basic Components of (USB-) MEA-Systems
Multi Channel Systems MCS GmbH provides complete solutions for data acquisition, recording,
stimulation, and perfusion from up to 256 channels, data analysis and export. The MEA
(microelectrode array) product line is intended for extracellular electrophysiological recordings
in vitro. Applications include whole-heart preparations; acute brain, heart, and retina slices,
cultured slices, and dissociated neuronal or cardiomyocyte cell cultures.
In the following, all basic components that are part of all complete MEA-Systems are briefly
described.
A microelectrode array MEA is an arrangement of usually 60 electrodes (256 electrodes in
256MEA or 120 electrodes in MEA2100-120-Systems) that allows the simultaneous targeting
of several sites for extracellular stimulation and recording. Cell lines or tissue slices are placed
directly on the MEA, and can be cultivated for up to several months. Almost all excitable or
spontaneously active cells and tissues can be used. The temperature controller TC01/02
regulates via perfusion cannula PH01 the temperature. The fluid flow is controlled by the
peristaltic perfusion pump PPS2-System.
Raw data from the MEA electrodes are amplified by MEA1060 filter amplifiers with custom
bandwidth and gain, which are built very small and compact using SMD (Surface Mounted
Devices) technology. The small-sized MEA1060 amplifier combines the interface to the MEA
probe with the signal filtering and the amplification of the signal. The compact design reduces
line pick up and keeps the noise level down. All MEA1060 amplifiers are available either for
Inverted microscopes or for upright microscopes, MEA1060-Inv and MEA1060-Up, respectively.
The analog input signals are acquired and digitized either by the internal data acquisition
device in MEA2100-Systems and USB-MEA256-Systems or by an external USB based data
acquisition device in USB-ME-Systems.
68-pin MCS high grade cables in different length are used for connecting the MEA1060 amplifier,
the USB based data acquisition, and any additional devices. The supply power is distributed to the
amplifiers via the MCS high grade cable as well. Please note that you need an external power
supply PS40W if you use an USB-ME device for operating multiple amplifiers.
Recorded data is graphed, analyzed, and reviewed with the powerful and easy-to-use MC_Rack
program. You can export the data in standard formats to other programs with MC_DataTool.
The data acquisition programs Cardio2D and LTP-Director are specially developed for cardiac
and for neuronal applications.
7
2.2
System Configurations and optional Components
Multi Channel Systems MCS GmbH provides several complete standard configurations and custom
systems. This chapter will give an overview on different system configurations. Please see also
both handouts “MEA - System Suggestions” and “USB-MEA-Systems - System Suggestions”,
which show diagrams of several typical setups.
Please note that you have various options for setting up your personal (USB-) MEA-System
configuration. For more information about the scope of delivery of your system, please see
the separate shipping documents.
Please see the separate documentation for information on installing and operating the individual
components of your (USB-) MEA-System. All warnings and safety related information of the
separate documents apply and must be regarded.
Do not hesitate to contact MCS or your local retailer if you are interested in a particular setup
or if you have other questions.
2.2.1 Single Components for MEA-Systems
The following components are part of (USB-) MEA standard systems. Please make sure that
you have carefully studied the documentation on the single components before setting up
your system. All manuals can be found on the installation volume shipped with the system.
Updated versions can also be downloaded from the Multi Channel Systems MCS GmbH web site.
Web link to the manuals download page on the MCS web site:
http://www.multichannelsystems.com/downloads
Product
Manual / Reference
MEA2100-System in different configurations
MEA2100-System Manual
USB-MEA256-System for MEAs with 256 electrodes
USB-MEA256-System Manual
USB-ME64-, 128-, 256-Systems data acquisition for
one, two or four MEA1060 amplifiers
USB-ME64- 128- 256-System Manual
MEA1060 amplifier(s) with or without blanking
circuit for upright or Inverse microscopes with custom
bandwidth and gain settings
MEA Amplifier (with Blanking Circuit)
for Upright / Inverse Microscopes Manual,
MEA_Select Help
MEA probes
MEA Manual
Temperature controller TC01 or TC02
TC01/2 Manual
Perfusion cannula PH01 with programmable fluid
temperature (systems with enhanced perfusion E only)
PH01 Manual
Perfusion Peristaltic System PPS2 for perfusion
control (systems with enhanced perfusion E only)
PPS2 Manual
Magnetic Perfusion Holder MPH to fix the PH01
MPH Datasheet
MEA Signal generator MEA-SG for systems with 60,
120 or 256 electrodes
60-, 120-, 256MEA-SG Datasheet
Test-MEA for systems with 60, 120 or 256 electrodes
60-, 120-, 256-TestMEA Datasheet
Data acquisition and analysis software MC_Rack and
MC_DataTool
MC_Rack Manual / Help, MC_Rack
Tutorial
Data acquisition and analysis software LTP-Director
LTP-Director Manual / Help
Data acquisition and analysis software Cardio2D
Cardio2D Manual / Help
Appendix
2.2.2 MEA2100-System
The MEA2100-System is the latest development in the MEA product family of Multi Channel
System MCS GmbH. The MEA2100-System is a compact and portable stand-alone system with
integrated amplification, data acquisition, online signal processing, integrated stimulus generator
as well as temperature control.
The MEA2100-System consists of two main devices: The interface board with integrated signal
processor and the headstage equipped with amplifier, A / D converter and stimulus generator.
It is possible to connect one headstage only or two headstages to one interface board. If you
are dealing with two headstages, each headstage can be operated independently by opening
the data acquisition software MC_Rack, for example for each headstage separately.
It is a highly flexible system, using a headstage that will fit on Inverted and upright microscopes.
The MEA2100-System acquires data from up to 240 electrode channels, 120 channels per
headstage and a sampling frequency of 50 kHz per channel. Eight additional analog IN channels
are available, and a 16 bit digital IN / OUT channels. The digitized electrode data is transmitted
to the connected interface board via eSATAp cable. The connection between interface board
and computer uses a high speed USB connection. Thus, it is possible to use any computer as
a data acquisition computer, also a laptop.
Data is recorded, stimulated, analyzed, graphed and reviewed with the powerful and easy-to-use
MC_Rack program from Multi Channel Systems MCS GmbH. The control functions of the internal
stimulator are also integrated into MC_Rack. The MC_Rack data format can be converted in other
standard formats with the software MC_DataTool.
2.2.3 USB-MEA256-System
The USB-MEA256-System is a complete system for in vitro recording from a special
microelectrode array with 256 electrodes (256MEA). The USB-MEA256-System is a compact
and portable stand-alone solution for MEA recordings with integrated amplification, data
acquisition, and analog / digital conversion. The system acquires data from up to 252 electrode
channels, four additional analog channels, and 16 digital in / out channels. The digitally converted
electrode signals are transmitted to any data acquisition computer via USB 2.0 (High Speed).
2.2.4 USB-MEA-Systems
The USB-MEA60-System is a complete system for in vitro recording from microelectrode arrays
(MEAs). The external data acquisition device USB-ME64 acquires data from up to 60 MEA
electrodes and four additional analog channels. An audio channel and 16 digital in / out channels
are available. The device has an integrated analog-digital converter board. The digitally converted
electrode signals are transmitted to any data acquisition computer via USB 2.0 (High Speed).
The USB-MEA120-System is a complete system for in vitro recording from microelectrode arrays
(MEAs). The external data acquisition device USB-ME128 acquires data from up to 120 MEA
electrodes and four additional analog channels. An audio channel and 16 digital in / out channels
are available. The device has an integrated analog-digital converter board. The digitally converted
electrode signals are transmitted to any data acquisition computer via USB 2.0 (High Speed).
The USB-MEA240-System is a complete system for in vitro recording from microelectrode arrays
(MEAs). The external data acquisition device USB-ME256 acquires data from up to 240 MEA
electrodes and four additional analog channels. An audio channel and 16 digital in / out channels
are available. The device has an integrated analog-digital converter board. The digitally converted
electrode signals are transmitted to any data acquisition computer via USB 2.0 (High Speed).
9
2.2.5 MEA1060-BC Amplifier with Blanking Circuit
All USB-ME-Systems are available with MEA1060 amplifiers for upright or inverted microscopes.
Amplifiers with MEA1060-BC amplifiers with blanking circuit featuring stimulus artifact
suppression. Electrodes can be grounded or selected for recording or stimulation with
the MEA_Select program.
2.2.6 Enhanced Perfusion System (- E)
A perfusion system is required especially for recordings from acute slices. All systems can be
upgraded with an enhanced perfusion system (indicated by the code E), featuring a perfusion
cannula PH01 with programmable fluid temperature and a two-channel temperature controller
TC02 for controlling both the MEA culture chamber temperature and the fluid temperature in
parallel. The PPS2 Perfusion Peristaltic Pump provides the fluid flow.
2.3
Accessories
MCS provides a wide range of accessories that will make your (USB-) MEA-System even more
efficient and convenient to use. All accessories are perfect for use with MCS products, but are
easily adaptable to custom systems as well. Some accessories are included in complete systems,
other have to be ordered separately.

1- or 2-channel Temperature controller for MEAs and for perfusion cannula PH01.

Peristaltic perfusion system PPS2 for the perfusion of biological samples.

Perfusion cannula PH01 for temperature controlled perfusion of the slice.

Magnetic perfusion holder MPH to fix the perfusion cannula PH01.

Magnetic metal plates MP and plates with M3 threads for positioning and tightly fixing tools
next to the MEA.

Digital in / out extension for connecting other devices to single digital input and output
channels of the data acquisition devices (up to 16 inputs and 16 outputs), for example,
for synchronizing stimulation and recording, or for applying feedback.

External power supply PS40W for use with the USB-MEA-Systems or for custom setups.
Appendix
2.4
Add-ons for Stimulation
In MEA2100-Systems the stimulation unit is already integrated. For all other systems MCS
provides general purpose stimulators like the advanced 4000 series with 2, 4 or 8 channels.
The flexible MC_Stimulus II software enables complex stimulus waveforms in current and
voltage mode. Waveforms designed in the program or imported from an external file are
converted by the STG into pulses, which are sent to the amplifier to stimulate electrodes.
The operating of the STG 4000 series is possible in download and streaming mode that
means continuous down streaming of pulses from connected computer.
Stimulus isolation units are integrated in the STG for each channel. Thus, no additional
stimulus isolations units are required.
Trigger in- and outputs are available for an exact timing of stimulation and for controlling
other instruments by TTL pulses. For example, stimulation and recording can be synchronized
with a digital trigger signal (TTL) sent from the Sync Out output of the stimulus generator to
the USB devices via digital inputs.
With the advanced MEA preamplifier with blanking circuit (BC), you will be able to ground
electrodes or select electrodes for recording and stimulation by software control MEA_Select
from the data acquisition computer. Stimulus artifacts and amplifier saturation are effectively
prevented with a blanking circuit.
11
2.5
Setting up the (USB-) MEA-System
Warning: Please read the separate manuals of all individual instruments before installation,
especially the warnings and safety information. Make sure all devices are switched off before
you connect them to the power supply. Damage to the devices and even fatal injuries may
result from improper installation or use.
See also the documentation “Microelectrode Array Systems, System Suggestions” and
“Microelectrode Array USB-Systems, System Suggestions” with detailed diagrams and various
setup suggestions. Provide a power supply in the immediate vicinity of the installation site.
1. Place all devices on a stable and dry surface, where the air can circulate freely and the devices
are not exposed to direct sunlight.
2. Set up the computer with installed MC_Rack program.
3. Set up the MEA amplifier as described in the amplifier`s manual.
4. Set up all other system components as described in the separate manuals.
With MEA2100-System:
5. Connect the headstage of the MEA2100-System via eSATAp cable to the interface board.
6. Connect the interface board via power supply unit to a power outlet.
7. Connect the interface board with an USB 2.8 A-mini B cable to the computer.
8. Ground the system.
With USB-ME64 / 128 / 256 device:
5. Connect the MEA amplifier(s) to the front panel of the USB-ME64 / 128 / 256 device with
3 m 68-pin MCS standard cable (C68x3M).
6. Connect the USB output connector of the USB-ME64 / 128 / 256 device to a free USB 2.0 port
of the data acquisition computer. It is not recommended to use a hub.
7. Connect the USB-ME64 / 128 / 256 device via power supply unit to a power outlet of the same
electrical system as the other components of the setup, for example, the computer or shielding.
8. Ground the system.
With USB-MEA256 device:
5. Connect the USB output connector of the USB-MEA256 device to a free USB 2.0 port
of the data acquisition computer. It is not recommended to use a hub.
6. Connect the USB-MEA256 device via power supply unit to a power outlet of the same electrical
system as the other components of the setup, for example, the computer or shielding.
7. Ground the system.
8. Optional: Connect the internal heating element to the temperature controller’s output channel
(D-Sub9 connector) with the integrated cable. Do not connect the heating element cable to the
computer!
Appendix
3
Extracellular Recording from MEAs
3.1
Introduction
Over the last 30 years, non-Invasive extracellular recording from multiple electrodes has
developed into a widely used standard method. Systems and methods have been greatly
improved, leading to more features, higher throughput, and lower costs. Almost all excitable
or electrogenic cells and tissues can be used for extracellular recording in vitro, for example,
central or peripheral neurons, heart cells, retina, or muscle cells.
3.2
Background
The semi permeable lipid bilayer cell membrane separates different ion concentrations (charges)
on the inner and outer side of the membrane. Therefore, the cell membrane has the electrical
properties of a plate capacitor. The electrochemical gradient results in a membrane potential
that can be measured directly with an intracellular electrode. When ion channels are opened
due to chemical or electrical stimulation, the corresponding ions are moving along their
electrochemical gradient. In other words, the resistance of the membrane is lowered,
resulting in an inward or outward flow of ions, measured as a transmembrane current.
The extracellular space is conductive as well, and though the resistance is very low, it is not zero.
According to Ohm's law (U=R*I), the extracellular current results in a small voltage that can be
measured with extracellular electrodes. Extracellular signals are smaller than transmembrane
potentials, depending on the distance of the signal source to the electrode. Extracellular signal
amplitudes decrease with increasing distance of the signal source to the electrode. Therefore,
a close interface between electrode and cell membrane is very important for a high
signal-to-noise ratio.
The transmembrane current and the extracellular potential follow the same time course and
are roughly equal to the first derivative of the transmembrane potential.
A microelectrode array (MEA) is an arrangement of several (typically 60) electrodes allowing
targeting several sites for stimulation or recording at once.
The following components are important for an extracellular recording system:

Signal source (cells / tissue);

Cell / sensor interface;

Biosensor (MEA);

Filter amplifier (MEA2100, USB-MEA256, MEA1060);

Recording hardware (MEA2100, USB-ME or USB-MEA devices) and software
(MC_Rack, LTP-Director, Cardio2D).
13
3.3
Signal Types
3.3.1 Single Unit Activities
Usually several cells are plated onto a MEA. The waveform of a single unit spike depends on
the signal source, the geometry of the extracellular space, and the distance of the signal source
to the electrode. The property of a waveform derived from a single neuron is reproducible over
time and therefore specific for that neuron. That is, the differences of waveforms from separate
signal sources can be used to distinguish the activities and to sort spikes into single unit spikes.
Thus, you can acquire single unit data from multiple cells in parallel by recording from a single
electrode.
You have to discriminate between independent activities and network responses. Responses
of cells on a MEA triggered by a chemical, electrical, or light stimulus can be either statistically
independent or show a specific pattern. The latter is quite interesting for studying the role of
cells and different tissues in a pathway. MEA recording allows such studies under controlled
experimental settings and is much easier and less labor intensive than an in vivo experiment.
3.3.2 Local Field Potentials
If the dendrite soma axes of the active cells are aligned, the waveforms from multiple units on a
MEA overlay and from a compound potential, or local field potential (LFP). The higher the activity,
that is, the spike rate, the higher is the amplitude of the LFP. A modulation of the stimulus results
in a higher frequency of action potentials that will result in a graded multiunit response.
LFPs often show a high signal-to-noise ratio, which is very beneficial for the analysis.
If dendrites are arranged in a nonparallel or radial fashion forming a closed field, the waveforms
may cancel each other out, when the neurons fire in synchrony.
3.4
Recording and Stimulation
The MEA sensor is placed directly into the small sized MEA amplifier or MEA2100 headstage.
When the amplifier is closed, the contact pins in the lid of the amplifier are pressed onto the
MEA contact pads. The very close location of the amplifier to the MEA sensor is very favorable
concerning a high signal-to-noise ratio.
If you use the MEA amplifier with blanking circuit, MEA_Select software control allows to select
any electrode on a MEA for stimulation and recording. In MEA2100 headstages the blanking
circuit feature is always provided. A user defined, typically 500 μs long, blanking signal switches
off stimulating electrodes during stimulation and thus removes stimulus artifacts.
With the MEA_Select software, it is easy to change the electrode selection during the experiment,
for example, to use stimulation electrodes for recording and vice versa. It is also possible to use
the same electrode for recording shortly after stimulation provided that you use a dedicated
biphasic pulse protocol that compensates for the slight DC offset that a stimulation electrode
always shows after stimulation. When using MEA2100-Systems the MEA_Select software is already
included in the data acquisition program MC_Rack, Cardio2D or LTP-Director, you do not have to
change the software program for blanking. Please read the MEA2100-System manual.
Appendix
3.4.1 Microelectrode Arrays (MEAs)
The recording field of a standard MEA with 60 electrodes is a square grid of 8 x 8 electrodes
with a total length between 120 μm to 5 mm in the middle of a circular, which is about 2 cm
wide. 256MEAs for USB-MEA256-Systems have 256 electrodes in a 16 x 16 electrode grid,
120MEAs for MEA2100-120-Systems have 120 electrodes in a 12 x 12 grid, perforated MEA32S12Lx for MEA2100-32-System have 32 recording and 12 stimulation electrodes. It is possible to glue
a culture chamber onto the MEA, made of plastic (Macrolon) or of glass.
The layout of the MEA electrodes follows the scheme of a standard grid: The first digit is the
column number, and the second digit is the row number. For example, electrode 23 is positioned
in the third row of the second column. The numbering follows the standard order from left
to right, and from top to bottom. This numbering is used in the documentation of the MEAs,
the MEA amplifiers, MEA2100 headstages, and in the data acquisition programs MC_Rack, LTPDirector, and Cardio2D. That means, if you want to record data from electrode 23, you choose
channel 23 for setting up the channel layout map. Please make sure that the appropriate 2dimensional data source setup ”2 dim. (MEA)” or “Configuration” selected in the data source
settings of MC_Rack. For more details, please refer to the MC_Rack help or manual.
Microelectrode arrays are available in various configurations:

Different electrode layout grids (8x8, 6x10, High Dense 2x(5x6), 4 Quadrants, Hexa, 6wellMEAs);

Different electrode diameters and spatial resolutions;

Different electrode materials (Titanium nitride, gold or PEDOT);

Opaque (titanium) or transparent (indium tin oxide) tracks and contact pads.
For more information on MEA types, electrode layouts, MEA handling, coating and cleaning,
please refer to the MEA manual.
3.4.2 Signal Amplification and Filters
The standard MEA amplifier combines the probe interface with a band pass filter and the signal
amplification in one instrument.
The MEA1060-BC amplifier with blanking circuit is a 60-channel preamplifier with a broad
bandwidth. Filter specifications and gain are defined by the following filter amplifier.
Different filter settings are used to enhance the signal-to-noise ratio. The pass band of the filter
amplifier depends on the signal type. It is generally useful to filter the data with a cutoff at the
highest signal frequency.
For slow signals like field potentials, a bandwidth of 1 to 300 Hz is appropriate. If you like to
record fast signals like spikes, a pass band of 300 Hz to 3 kHz is suitable. Cardiac signals have
fast and slow components; therefore, you usually need a wider bandwidth of 1 Hz to 3 kHz.
Multi Channel Systems MCS GmbH provides custom amplifiers with a bandwidth of your choice,
from 0.1 Hz to 10 kHz. Please note that it is often wise to acquire the data with a broadband
amplifier and use the digital filter of the MC_Rack program to change the pass band and filter
the raw data. This way, you are much more flexible in designing your experiments. As a further
advantage, you can see the original (not filtered) data as well. This is especially important because
all filters are known to distort signals. On the other hand, you may need a higher sampling rate to
avoid aliasing, and the signal-to-noise ratio is lower. See also the chapter "Data Acquisition" for
more information.
15
The MEA2100 amplifier with blanking circuit is a 60- or 120-channel headstage with a hardware
defined bandwidth. Default settings for the hardware filter will be 1 Hz to 3 kHz. However,
a different bandwidth can be achieved by an additional software program, called “MEA2100
Configuration”, no hardware modification is necessary. The hardware defined bandwidth marks
the upper and lower limit of the frequency range, fine tuning within this range can be done with
software filters in MC_Rack.
Note: To change the hardware filter settings without changing the firmware, you can use the add
on software “MEA2100 Configuration”.
Please see the manual for the “MEA2100 Configuration” software in the Appendix. The MEA2100System has a fixed hardware gain of 10. However, due to the large resolution of 24 bit, it is
possible to adjust the actual signal input range used for recording in a wide range from +/- 812 μV
to a maximum of +/- 227 mV. The 24 bits are distributed among this complete voltage range.
The standard gain of a MEA1060 amplifier is 1200, which is fine for most applications, but MCS
also provides amplifiers with a gain of your choice (from 100 to 5000) as well. In MEA2100 the
bandwidth of the filter settings on the headstage are adjustable via the software program
“MEA2100 Configuration”. For large signals (for example, from heart preparations), you need
a lower gain to prevent a saturation of the amplifier. Please note that the gain is a fixed hardware
property; and that you cannot change the gain of the amplifier by software controls.
Please note that the ratio of the output signal to the input signal, that is, the gain, is not
a fixed parameter for the complete bandwidth. The gain that was specified for the amplifier,
for example, 1200, is not fully reached at the borders of the amplifier's pass band. The general
rule is, that at the lower and upper limit of the frequency band, the gain is 2 / 2 , that is
approximately 70 %, of the full gain. Therefore, you should use a bandwidth that is at a safe
distance of the signals of interest. Outside the pass band, the gain decreases with the frequency
and finally approaches zero.
For information on the gain and filters of the (USB-) MEA amplifiers, please see the appropriate
amplifier manual. For more information on gain and filters in general, please refer to standard
literature or contact your local retailer.
3.4.3 Data Acquisition
Recording from up to 240 channels is easy with the respective hardware and the MC_Rack
software or with LTP-Director, Cardio2D. The MEA2100-System and USB-MEA data acquisition
devices provide the possibility to record from up to 240 electrodes with the designated software.
You configure the input voltage range from +/- 400 mV to +/- 4 V and the sampling rate with
software control in the MC_Rack program. Please refer to the MC_Rack help or manual for
more information.
The input voltage range affects your dynamic range, that is, the lower the input voltage range,
the higher is the voltage resolution.
DC Offset correction
An offset correction is generally not necessary, because the intrinsic DC offsets of the MCS
amplifier outputs and the data acquisition are very low in comparison to the signals of interest.
You can use the MC_Rack offset correction feature to remove even this low offset and reset
all channels to zero. Please refer to the MC_Rack help or manual for more information.
Note: If you observe a large offset on any channel(s), you should contact your local retailer for
troubleshooting. The offset correction is not intended for removing large offsets, because the
offset correction will decrease the input voltage range.
Appendix
Sampling rate
It is recommended to adjust the sampling rate according to your signals, because the higher
the sampling rate, the bigger the file size will be.
As a rule of thumb, the sampling rate should equal five times the highest signal frequency for
a good digitized representation of the continuous analog signals. If the sampling rate is too low,
you will miss signals and / or see artifacts. Considering the preceding statements about filtering
data, you would for example use a 5 kHz sampling rate when using a MEA amplifier with a cutoff
frequency of 1 kHz.
Please note that if you use a broadband amplifier and a digital filter, you may have to use an even
higher sampling rate.
This is the case because the whole amplifier bandwidth is recorded and then high frequency noise
is removed with a digital low pass filter after recording. Frequencies (noise) that are above half
the sampling rate (for example above 2.5 kHz at a 5 kHz sampling rate) will be transformed into
lower frequencies. This is called aliasing. This low frequency noise passes the digital “Low Pass”
filter and increases your noise level.
According to the Nyquist-Shannon sampling theorem, the sampling rate should equal twice the
bandwidth of the analog (hardware) low pass filter. The 1/2 bandwidth frequency is also called
Nyquist frequency. You may ignore this if saving hard disk space is more important for your
application than the noise level.
Aliasing
Note: The sampling frequency should be at least five times the highest signal frequency and
at least twice the bandwidth of the MEA amplifier.
Example:
You have a broadband MEA1060 amplifier with a bandwidth of 0.1 Hz to 10 kHz. The expected
signals have a maximum frequency of 1 kHz. Therefore, you want to filter the data with a digital
Low Pass filter and a cutoff frequency of 1 kHz. A sampling rate of 5 kHz (five times the highest
signal frequency) would be required for faithfully reproducing the signals, but you should use a
sampling rate of at least 20 kHz because the sampling rate should equal twice the bandwidth of
the analog filter, regardless of the digital filter properties.
If you have a MEA1060 amplifier with a cutoff frequency of 1 kHz instead, no digital filter would
be required, and a sampling rate of 5 kHz would be enough.
17
Software packages
With the high-performance data acquisition and analysis program MC_Rack, you can flexibly
manage all data streams. For example, you can display the raw data of all channels while
recording only the raw data of the channels of interest and the extracted parameters of all
channels. This saves computer performance and hard disk space.
MC_Rack is not limited to special applications, but can be flexibly adapted to a wide range
of applications.
Unlimited software licenses and support come free with the (USB-) MEA-System, and free
software updates lower the costs as well.
The MC_Rack “*.mcd” data format is supported by several third party programs for further
analysis. You can also easily convert recorded data to universal formats such as ASCII with
the MC_DataTool program.
The LTP-Director is an easy to use data acquisition and analysis program to do standardized
LTP (long term potentiation) and LTD (long term depression) recordings in hippocampal acute
slices. The program controls MEA amplifiers and stimulus generators from Multi Channel Systems
MCS GmbH from within one software.
The Cardio2D data acquisition and analysis program maps cardiac action potential spreading
in cell and tissue preparations. The software allows the mapping of cardiac excitation on
microelectrode arrays via local activation times, and spatial distribution of local activation times.
The spatial distribution of local field potentials and conduction velocity are measured, and false
colour maps and movies are generated. The Cardio2D software can be used in conjunction with
cardiomyocyte cultures, cardiac slices or with flexible MEAs for cardiac surface mapping. Perfect
for drug testing in primary cardiomyocytes, cardiac slices, whole heart surfaces and stem cell
derived cardiomyocytes.
3.5
Using the additional Analog Inputs
The additional analog inputs A1 to A4 of the data acquisition devices are intended for recording
additional information from external devices, for example, for recording patch clamp in parallel
to the MEA recording, for monitoring the temperature, or for recording voice. You could also use
the analog inputs for triggering the MEA2100 headstage or the USB devices, but please note that
the digital inputs are intended for accepting TTL pulses.
As the gain is generally completely different on the electrode inputs and on the additional analog
inputs, signals on the analog channels are recorded "as is", with no respect to the gain specified
in MC_Rack.
Recording Voice on Analog Inputs
Microphones do not generate enough voltage to directly connect them to the analog inputs.
You need to use some kind of amplifier that adapts the output voltage of the microphone to
the input voltage range of the data acquisition device.
You can then use one of the MC_Rack sound tools for replaying the recorded voice.
Please see the MC_Rack manual for more information.
Appendix
3.6
Digital Input / Output, System Synchronization
TTL stands for Transistor-Transistor Logic. A TTL pulse is defined as a digital signal for
communication between two devices. A voltage between 0 V and 0.8 V is considered
as a logical state of 0 (LOW), and a voltage between 2 V and 5 V means 1 (HIGH).
Warning: A voltage that is higher than +5 Volts or lower than 0 Volts, that is, a negative
voltage, applied to the digital input would destroy the hardware. Make sure that you apply
only TTL pulses (0 to 5 V) to the digital inputs.
3.6.1 Triggering the Data Acquisition and MC_Rack
The digital input accepts TTL pulses. This feature can be used for triggering the USB device
and MC_Rack, for example, for synchronizing stimulation and data acquisition.
For example, you can connect the Sync Out of a STG (stimulus generator) to one of the digital
input bits. If you use only one instrument for triggering, connect it to bit 0. In MC_Rack, add a
Trigger Detector to your virtual rack, and select the Digital Data D1 input stream as the Trigger.
Select the appropriate logical state (generally HIGH) for triggering. Mask all unused bits.
The standard settings of the Trigger Detector are for using bit 0.
Please see the documentation on the Recorder and on the Trigger Detector instrument and
on triggered data in the MC_Rack help or manual for more details.
Important: It is recommended to apply TTL pulses with a duration of at least 200 μs. Shorter
pulses may be ignored by the USB device. Please mask unused (not connected) digital input
channels in the MC_Rack program to ignore undefined states of the open inputs that can
cause unwanted trigger events. Please see the MC_Rack manual for more details.
3.6.2 Triggering other Instruments by MEA2100-System or USB-Device
The digital output of the USB device generates and sends 20 ms TTL pulses (0 V = LOW and
5 V = HIGH). This feature can be used to apply a feedback triggered by a signal or a parameter
stream.
For example, you can connect the “Trigger In” of a stimulus generator (STG) to the digital output
via the “Digital IN / OUT” connector. You can also use the digital output for a synchronization of
the (USB-) MEA-System with other systems, for example, for Calcium imaging or video tracking,
provided that the other system of choice is able to receive TTL pulses.
Please see the documentation on the digital output instrument in the MC_Rack help or manual
for more details.
19
4
Troubleshooting
4.1
About Troubleshooting
The following hints are provided to solve special problems that have been reported by users.
Most problems occur seldom and only under specific circumstances. Please check the mentioned
possible causes carefully when you have any trouble with the product. In most cases, it is only
a minor problem that can be easily avoided or solved.
If the problem persists, please contact your local retailer. The highly qualified staff will be glad
to help you. Please inform your local retailer as well, if other problems that are not mentioned
in this documentation occur, even if you have solved the problem on your own. This helps other
users, and it helps MCS to optimize the instrument and the documentation.
Please pay attention to the safety and service information in the separate manuals of the related
products and in the software help. Multi Channel Systems has put all effort into making the
product fully stable and reliable, but like all high-performance products, it has to be handled
with care.
4.2
Triggering / Digital Input does not Work
You have connected a TTL source (for example, the Sync Out of a stimulus generator) to the
digital input of the USB device or the interface board of the MEA2100-System, and configured
the virtual rack in MC_Rack for triggering displays or data acquisition by the TTL source, but you
do not see any sweeps.
Possible causes:
?
You have mismatched the digital input with the analog input. To test this, please add a Digital
Display to your virtual rack. You should see the trigger event as a logical state of HIGH (=1).
If you cannot see any signals, please add a Data Display to your rack and select the analog raw
data as the input stream. Make sure the channel layout map of the display shows all three / four
analog input channels (A1 to A4). If you have mismatched the inputs, you will now see the
(clipped) trigger events.
 Connect the TTL source to the “Digital In” of the USB device or to the interface board of the
MEA2100-System. If you have only one instrument for triggering recording (for example, the
Sync Out of a stimulus generator), connect it to bit 0 or to D0 IN.
 If you use a digital in / out extension (Di/o) accessory, you can connect up to 16 digital input
sources and up to 16 devices that you want to be triggered by the Digital Output instrument
of MC_Rack.
?
The TTL source does not generate true TTL signals (5 V), or the TTL pulse is shorter than 200 μs.
 The USB device can only accept TTL signals (CMOS 5 V TTL level) as a digital input stream.
A minimum TTL pulse of 200 μs is recommended. Otherwise, a detection of the trigger by
the USB device is not guaranteed.
?
The software settings for the Trigger Detector do not match with the hardware configuration.
 In MC_Rack, add a Trigger Detector to your virtual rack, and select the Digital Data D1 input
stream as the Trigger. Check the digital inputs and make sure that the same bit input that is
connected is selected in the software. (The standard settings of the Trigger Detector are for using
bit 0.) Mask all unused bits. Select the appropriate logical state (generally HIGH) for triggering.
Please see the MC_Rack help or manual for more details.
Appendix
4.3
Artifacts caused by Perfusion
You observe artifacts or an increased noise level after you started the perfusion.
Possible causes:
?
The artifacts correlate with an oscillation of the liquid level. The oscillation will cause the artifacts,
and also mechanically stress the biological sample.
 Use a bevelled tip for the perfusion in- and outlet. If the tip of the perfusion outlet has a blunt
end, there will be no continuous liquid flow, but instead, the following can be observed:
The liquid level will rise until it reaches the blunt tip; a discrete volume of liquid will be aspirated
in a short moment; the liquid level will rise again, and so on, leading to an oscillation. The tip
should be cut in an angle of about 45 °. You can also use commercially available metal needles
with a bevelled tip.
?
The perfusion is not appropriately grounded.
 Connect the perfusion line to the ground of the amplifier / setup. You should consider using
a metal cannula that can directly be connected to ground for the perfusion inlet and outlet.
You could also insert a metal wire that is connected to ground into the tubing.
?
The aspiration pump is oscillating.
 MCS recommends the use of the peristaltic perfusion system PPS2 for an open perfusion system.
The more rollers the pump head has, the lower are the oscillations of the fluid flow. For a
continuous perfusion in a closed system, you can also use an infusion-withdrawal syringe pump.
21
5
Appendix
5.1
Contact Information
Local retailer
Please see the list of official MCS distributors on the MCS web site.
User forum
The Multi Channel Systems User Forum provides an excellent opportunity
for you to exchange your experience or thoughts with other users worldwide.
Web link to the User Forum
Mailing List
If you have subscribed to the mailing list, you will be automatically informed
about new software releases, upcoming events, and other news on the product
line. You can subscribe to the list on the contact form of the MCS web site.
http://www.multichannelsystems.com/
Appendix
5.2
Ordering Information
Please contact your local retailer for pricing and ordering information.
5.2.1 MEA2100-Systems
Product
Product
Description
MEA2100 recordingSystem with 60
electrode channels
MEA2100-60System
Complete with MEA2100-60-headstage, MEA2100interface board, 5 MEAs, data acquisition computer
with preinstalled software, 1-channel temperature
controller, power supply, and accessories. USB High
Speed data transfer with a sampling rate of up to
50 kHz/channel.
MEA2100 recordingSystem with 60
electrode channels
MEA2100-60System-E
Complete with MEA2100-60-headstage, MEA2100interface board, 5 MEAs, data acquisition computer
with preinstalled software, 2-channel temperature
controller, heatable perfusion cannula with
temperature sensor, peristaltic perfusion system,
power supply, and accessories. USB High Speed
data transfer with a sampling rate of up to
50 Hz/channel.
MEA2100 recordingSystem with 120
electrode channels
MEA2100-2x60System
Complete with MEA2100-2x60-headstage,
MEA2100-interface board, 5 MEAs, data acquisition
computer with preinstalled software, 2-channel
temperature controller, power supply, and
accessories. USB High Speed data transfer with
a sampling rate of up to 50 kHz/channel.
MEA2100 recordingSystem with 120
electrode channels
MEA2100-2x60System-E
Complete with MEA2100-2x60-headstage,
MEA2100-interface board, 5 MEAs, data acquisition
computer with preinstalled software, two 2-channel
temperature controllers, two heatable perfusion
cannulas with temperature sensor, peristaltic
perfusion system, power supply, and accessories.
USB High Speed data transfer with a sampling rate
of up to 50 kHz/channel.
MEA2100 recordingSystem with 120
electrode channels
MEA2100-120System
Complete with MEA2100-120-headstage, MEA2100interface board, 5 MEAs, data acquisition computer
with preinstalled software, 1-channel temperature
controller, power supply, and accessories. USB High
Speed data transfer with a sampling rate of up to
50 kHz/channel.
MEA2100 recordingSystem with 120
electrode channels
MEA2100-120System-E
Complete with MEA2100-120-headstage, MEA2100interface board, 5 MEAs, data acquisition computer
with preinstalled software, 2-channel temperature
controller, heatable perfusion cannula with
temperature sensor, peristaltic perfusion system,
power supply, and accessories. USB High Speed
data transfer with a sampling rate of up to
50 kHz/channel.
23
MEA2100 recordingSystem with 120
electrode channels
MEA2100-60-2System
Complete with two MEA2100-60-headstages,
MEA2100-interface board, 5 MEAs, data acquisition
computer with preinstalled software, 2-channel
temperature controller, power supply, and
accessories. USB High Speed data transfer with
a sampling rate of up to 50 kHz/channel.
MEA2100 recordingSystem with 120
electrode channels
MEA2100-60-2System-E
Complete with two MEA2100-60-headstages,
MEA2100-interface board, 5 MEAs, data acquisition
computer with preinstalled software, two 2-channel
temperature controllers, two heatable perfusion
cannulas with temperature sensor, peristaltic
perfusion system, power supply, and accessories.
USB High Speed data transfer with a sampling rate
of up to 50 kHz/channel.
MEA2100 recordingSystem with 240
electrode channels
MEA2100-2x602-System
Complete with two MEA2100-2x60-headstages,
MEA2100-interface board, 5 MEAs, data acquisition
computer with preinstalled software, two 2-channel
temperature controllers, power supply, and
accessories. USB High Speed data transfer with
a sampling rate of up to 50 kHz/channel.
MEA2100 recordingSystem with 240
electrode channels
MEA2100-2x602-System-E
Complete with two MEA2100-2x60-headstages,
MEA2100-interface board, 5 MEAs, data acquisition
computer with preinstalled software, four 2channel temperature controllers, four heatable
perfusion cannulas with temperature sensor,
peristaltic perfusion system, power supply, and
accessories. USB High Speed data transfer with
a sampling rate of up to 50 kHz/channel.
MEA2100 recordingSystem with 240
electrode channels
MEA2100-120-2System
Complete with two MEA2100-120-headstages,
MEA2100-interface board, 5 MEAs, data acquisition
computer with preinstalled software, 2-channel
temperature controller, power supply, and
accessories. USB High Speed data transfer with
a sampling rate of up to 50 kHz/channel.
MEA2100 recordingSystem with 240
electrode channels
MEA2100-120-2System-E
Complete with two MEA2100-120-headstages,
MEA2100-interface board, 5 MEAs, data acquisition
computer with preinstalled software, two 2-channel
temperature controllers, two heatable perfusion
cannulas with temperature sensor, peristaltic
perfusion system, power supply, and accessories.
USB High Speed data transfer with a sampling rate
of up to 50 kHz/channel.
MEA2100 recordingSystem with 32
electrode channels
MEA2100-32System
Complete with MEA2100-32-headstage with
integrated perfusion element, MEA2100-interface
board, 5 MEAs, data acquisition computer with
preinstalled software, 1-channel temperature
controller, heatable perfusion cannula with
temperature sensor, power supply, and accessories.
USB High Speed data transfer with a sampling rate
of up to 50 kHz/channel.
Appendix
MEA2100 recordingSystem with 64
electrode channels
MEA2100-2x32System
Complete with MEA2100-2x32-headstage with
integrated perfusion elements, MEA2100-interface
board, 5 MEAs, data acquisition computer with
preinstalled software, 2-channel temperature
controller, 2 heatable perfusion cannulas with
temperature sensor, power supply, and accessories.
USB High Speed data transfer with a sampling rate
of up to 50 kHz/channel.
MEA2100 recordingSystem with 64
electrode channels
MEA2100-32-2System
Complete with two MEA2100-32-headstages with
integrated perfusion elements, MEA2100-interface
board, 5 MEAs, data acquisition computer with
preinstalled software, 2-channel temperature
controller, 2 heatable perfusion cannulas with
temperature sensor, power supply, and accessories.
USB High Speed data transfer with a sampling rate
of up to 50 kHz/channel.
MEA2100 recordingSystem with 64
electrode channels
MEA2100-2x322-System
Complete with two MEA2100-2x32-headstages with
integrated perfusion elements, MEA2100-interface
board, 5 MEAs, data acquisition computer with
preinstalled software, two 2-channel temperature
controllers, 4 heatable perfusion cannulas with
temperature sensor, power supply, and accessories.
USB High Speed data transfer with a sampling rate
of up to 50 kHz/channel.
5.2.2 USB-MEA-Systems
Product
Product Number
Description
MEA recording system with
integrated data acquisition,
filter amplification, and data
transfer via USB 2.0 High Speed
to any computer, 252 electrode
channels
USB-MEA256-System
Complete with 5 x 256MEAs,
data acquisition computer,
software package, and
accessories
MEA recording system with
integrated data acquisition,
filter amplification, and data
transfer via USB 2.0 High Speed
to any computer, 252 electrode
channels
USB-MEA256-System-E
Complete with 5 x 256MEAs,
TC02 and PH01, data
acquisition computer,
software package,
and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
240 electrode channels
USB-MEA240-Inv-4-System
Complete with 5 MEAs,
4 x MEA1060-Inv amplifier,
2 x TC02, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
240 electrode channels
USB-MEA240-Up-4-System
Complete with 5 MEAs,
4 x MEA1060-Up amplifier,
2 x TC02, and accessories
25
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
240 electrode channels
USB-MEA240-Inv-4-System-E
Complete with 5 MEAs,
4 x MEA1060-Inv amplifier,
4 x TC02,
4 x PH01,
4 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
240 electrode channels
USB-MEA240-Up-4-System-E
Complete with 5 MEAs,
4 x MEA1060-UP amplifier,
4 x TC02,
4 x PH01,
4 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
240 electrode channels
USB-MEA240-Inv-4-BCSystem
Complete with 5 MEAs,
4 x MEA1060-INV-BC amplifier,
2 x TC02, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
240 electrode channels
USB-MEA240-Up-4-BCSystem
Complete with 5 MEAs,
4 x MEA1060-UP-BC amplifier,
2 x TC02, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
240 electrode channels
USB-MEA240-Inv-4-BCSystem-E
Complete with 5 MEAs,
4 x MEA1060-INV-BC amplifier,
4 x TC02,
4 x PH01,
4 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
240 electrode channels
USB-MEA240-Up-4-BCSystem-E
Complete with 5 MEAs,
4 x MEA1060-UP-BC amplifier,
4 x TC02,
4 x PH01,
4 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
120 electrode channels
USB-MEA120-Inv-2-System
Complete with 5 MEAs,
2 x MEA1060-INV amplifier,
1 x TC02, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
120 electrode channels
USB-MEA120-Up-2-System
Complete with 5 MEAs,
2 x MEA1060-UP amplifier,
1 x TC02, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
120 electrode channels
USB-MEA120-Inv-2-System-E
Complete with 5 MEAs,
2 x MEA1060-INV amplifier,
2 x PH01,
2 x TC02,
2 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
120 electrode channels
USB-MEA120-Up-2-System-E
Complete with 5 MEAs,
2 x MEA1060-UP amplifier,
2 x PH01,
2 x TC02,
2 x PPS 2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
120 electrode channels
USB-MEA120-Inv-2-BCSystem
Complete with 5 MEAs,
2 x MEA1060-INV-BC amplifier,
1 x TC02, and accessories
Appendix
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
120 electrode channels
USB-MEA120-Up-2-BCSystem
Complete with 5 MEAs,
2 x MEA1060-UP-BC amplifier,
1 x TC02, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
120 electrode channels
USB-MEA120-Inv-2-BCSystem-E
Complete with 5 MEAs,
2 x MEA1060-INV-BC amplifier,
2 x PH01,
2 x TC02,
2 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
120 electrode channels
USB-MEA120-Up-2-BCSystem-E
Complete with 5 MEAs,
2 x MEA1060-UP-BC amplifier,
2 x PH01,
2 x TC02,
2 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
60 electrode channels
USB-MEA60-Inv-System
Complete with 5 MEAs,
1 x MEA1060-INV amplifier,
1 x TC02, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
60 electrode channels
USB-MEA60-Up-System
Complete with 5 MEAs,
1 x MEA1060-UP amplifier,
1 x TC02, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
60 electrode channels
USB-MEA60-Inv-System-E
Complete with 5 MEAs,
1 x MEA1060-INV amplifier,
1 x PH01
1 x TC02,
1 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
60 electrode channels
USB-MEA60-Up-System-E
Complete with 5 MEAs,
1 x MEA1060-INV amplifier,
1 x PH01
1 x TC02,
1 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
60 electrode channels
USB-MEA60-Inv-BC-System
Complete with 5 MEAs,
1 x MEA1060-INV-BC amplifier,
1 x TC01, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
60 electrode channels
USB-MEA60-Up-BC-System
Complete with 5 MEAs,
1 x MEA1060-UP-BC amplifier,
1 x TC01, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
60 electrode channels
USB-MEA60-Inv-BC-System-E
Complete with 5 MEAs,
1 x MEA1060-INV-BC amplifier,
1 x PH01
1 x TC02,
1 x PPS2, and accessories
MEA recording system with
integrated data acquisition,
and data transfer via USB 2.0
High Speed to any computer,
60 electrode channels
USB-MEA60-Up-BC-System-E
Complete with 5 MEAs,
1 x MEA1060-UP-BC amplifier,
1 x PH01
1 x TC02,
1 x PPS2, and accessories
27
5.2.3 Stimulus Generators
Product
Product Number
Description
2-Channel stimulus
generator
STG4002
4-Channel stimulus
generator
STG4004
8-Channel stimulus
generator
STG4008
4000 series: General-purpose stimulus generator
for current and voltage-driven electrical stimulation,
with integrated stimulus isolation unit for each
output channel. Operating in download and
streaming mode (continuous down streaming of
pulses from connected computer). MC_Stimulus II
program with advanced features.
5.2.4 MEA Amplifiers
Product
Product Number
Description
MEA amplifier for Inverted
microscopes
MEA1060-Inv
MEA amplifier for upright
microscopes
MEA1060-Up
Probe interface and 60 channel pre- and
filter amplifier with custom gain and
bandwidth.
MEA amplifier with blanking
circuit for Inverted microscopes
MEA1060-Inv-BC
MEA amplifier with blanking
circuit for upright microscopes
MEA1060-Up-BC
Probe interface and 60 channel pre- and
filter amplifier with custom gain and
bandwidth. The blanking circuit prevents
the amplifier from getting saturated and
thus prevents stimulus artifacts.
5.2.5 Accessories
Product
Product
Number
Description
Peristaltic Perfusion
System
PPS2
The peristaltic pump PPS2 with software control is
developed for the perfusion of biological samples.
Magnetic Perfusion
Holder
MPH
The magnetic perfusion holder is for fixing the perfusion
cannula PH01.
Holder with M3 threads
MPM3
For fixing tools with M3 threads next to the MEA.
1-Channel temperature
controller
TC01
2-Channel temperature
controller
TC02
PID based technology, set-point temperature reached fast
within 30 s to 5 minutes, control temperature range from
ambient temperature to +50 °C.
Perfusion cannula
PH01
Digital in / out extension
Di/o
For connecting other devices to single digital input and
output channels via BNC connectors (up to 16 inputs and
16 outputs), for example for synchronizing stimulation
and recording, or for applying feedback.
External power supply
PS40W with 40 W power
and ±7 V output voltage
PS40W
External power supply for supplying power to MEA1060
amplifiers and / or other peripheral devices.
Temperature can be programmed with the temperature
controller TC01 or TC02.