PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION MRC.VER.01-6.12

PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION  MRC.VER.01-6.12
Operation Manual for
THERMAL CYCLER
PCRSG-500
PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION
3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 [email protected]
MRC.VER.01-6.12
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1.
2.
Maintenance
Troubleshooting
1.
2.
Guarantee Content
Guarantee Scope
Appendix B Instrument Guarantee
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2 Safety notice
Please read the manual carefully before operating the instrument,
the wrong operation may be hazardous to the operator or the
instruments itself.
① Operating safety
The power cord for the instrument should be grounded reliably. The instrument
adopts three-core grounding plug, and should be connected with appropriate
power socket. Before connecting to the power, you must make sure that the
voltage and power load of the socket is in accordance with the capacity on the
instrument's nameplate.
Please change the power cord with the same type if the skin of the cord is found to
be broken. Nothing is allowed to be place on the power cord and the cord shall
not be placed in the area that may be easy approaching by people. Be sure to hold
the plug tightly while inserting, pulling of the cord directly is forbidden.
While the instrument is in operation, the sample plate block and the heat lid will
produce high temperature, so no part of human body is allowed to touch the
metal block or the heat lid while the instrument is in operation in case of any
scalding.
For the good ventilation, no articles are allowed to be placed within 30cm around
the instrument.
② Maintenance Safety
The block and the heat lid of the instrument need regular cleaning to guarantee
the precision of the experiment. Before cleaning, the sample plate block is suggested
to be taken out for soft scrubbing by the clean soft cloth stained with the pure alcohol
in appropriate amount. No corrosive cleaning agent is allowed for use and dripping
the cleaning agent into the taper hole is forbidden in case of any damage to the
instrument. And the sample plate block should be put back into the instrument after
cleaning.
Please cut off the power at once and stop the experiment and then immediately
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contact the distributor or the professional maintenance personnel in case of any
occurrence of the following situations:
Any reagent, rain water or other liquid enters into the instrument.
The instrument falls from a high place or the instrument shell is damaged.
The operating instrument produces the abnormal noise or the pungent odor.
The features of the instrument change apparently, such as failures of startup or
shutdown or failure to run user program, etc.
Notice: For your personal safety and the normal operation of the
instrument, please do not disassemble the instrument by yourself.
Any such behavior may endanger your safety and deprive your
guarantee right of the instrument.
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(6)Control panel (7)Screen (8) Key pad (9) Power switch (10) Power socket (11) Fuse
socket (12) Network interface (13) USB interface (14) JTEG interface (15) Shell (16)
Ventilation window (17) LOGO (18) Lid wrench (19) Block selecting knob (20) Block
selecting indicator
2.2 Lid-opening angle
This type of instrument takes a leading role in adopting the hinge joint
technology, enabling the upper lid of the instrument to stay at any angle according
to requirement and solving such problems brought by the traditional method of
relying on spring elasticity to open the lid as unreliability, instability, large inertia and
short life etc.
Pull the wrench to
push
the
lid
backward
Stop at any angle
according
to
requirements
As indicated in the above two figures, when the wrench is raised the buckle will be
released, and then the user can set the upper lid at any angle according to their
requirement. Humanistic structure endows the product more beautiful and practical
characteristics, and guarantees the safety and convenient operation.
Notice: According to the instrument characteristics, the open angle scope
between upper lid and the shell is 0°~ 90°, please keep it within the scope to
prevent any damage to the instrument.
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2.3 Hot Lid positions
Frequently, experiments need different reaction tubes, that is, use different blocks
to match the tubes. The design of the heat lid gear positions realize the heat lid to
match different sample plate blocks just by turning the knob to the appropriate
position.
As the above figures indicate that the mark of 0.5, 0.2,384 (clockwise) on the panel
respectively corresponds to the gear position of tube 0.5ml, 0.2ml and 384sample plate
blocks. If you need the 0.2ml tube for experiment, you only need to change the block
with 0.2ml block (See 2.4 Module for details), then switch the gear to the mark of 0.2,
and then you can start your experiment. The design abandons the traditional practice
of changing heat lid/or even instrument.
Notice: According to characteristics of the instrument, the turning range of the
switch is between 0.5 to 384, do not try to turn the switch out of this range.
2.4 Module
For the need of users to use tubes of different specifications for experiment in the
same instrument, and for the convenience of users to change relevant parts directly
and effectively, the instrument adopts the portable design with auto-lock function to
make the instrument meet the experiments of different tubes on the condition of
changing least parts, which fully reflects the humanistic design of the instrument.
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Block components
(1) Handle
(2) Magnetic steel
(3) Frame
(4) Block
(5) Heat sink
The above figure indicates that the integrated block parts design of the instrument
greatly improves operational convenience no matter in installation or replacement,
and the overall dimension, assembly process and installation method of all the blocks
of all specifications are the same (The block parts of different specifications differ with
blocks corresponding to tubes of different specifications). And this guarantees that
users can change or install the block in a fast and high-efficient way only by choosing
the block with specification needed.
Figure 1 show how to change the block unit. When changing the block, the user
shall pull the handle upward, when the handle and the shell are in a vertical state, the
block will be unlocked, and then the user can easily take out the block part by pulling
the handle vertically. Figure 2 show how to insert the block, when the user confirm the
block part of certain specifications needed, the user only needs to hold the block’s
handle. Vertically insert the block in position, then put the handle down to complete
the operation.
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Step 1: Move the handle to the state vertical to the shell to make the block parts
unlocked. (Figure 3)
Step 2: Move the block parts upward slightly, and lay it in a safe place after the block
parts are completely off the shell. (Figure 4)
Step 3: Choose the block parts of specifications needed and gently put them into the
shell vertically.
Step 4: After the block parts completely contact the shell, press back the handle
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Style of temperature control: block; tube calculated
Maximum number of program units: 1000
Maximum number of cycles: 255
,
Graphical interfaces:5.7inch 640*480pels LCD
、 RS232、 RJ45
Communication interface: USB2.0
Exterior size: 380mm(L)*270mm(W)*250mm(H)
Net weight: 7.8kg
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Chapter 3 Instructions
Optical disk contains ActiveSync software, oprating environment:
Win9xMeNT2000XP2003;VistaWin7 System is Windows Mobile.
3.1 Program installation
Step 1、Install ActiveSync software in PC. (Windows Mobile of Vista、Win7 is updated
version of ActiveSync)
Step 2、Connect PC and PCR by USB cable.
Step 3、Right click“My Computer”Properties“Hardware”“Device Manager”
“Windows CE USB Devicesright click”USB Device” ”Update Driver…”,to
install USB driver.(Figure 3-1, 3-2)
3-1
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3-2
Step 4、Click”Browse”and find out the file containing USB driver.(Figure 3-3、3-4)
3-3
3-4
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Step 5、After selecting “No” in “New Partnership”, (Figure 3-5)PC will be
connected to PCR.(Figure 3-6)
3-5
3-6
Step 6、Nandflash appears in PC, double click to enter.
Step 7、In Nandflash, replace old TempertureCtrl with new TemperatureCtrl and TCtrl,
then software upgrades successfully.
Step 8、Decompress installation package of upper computer in PC,click install and
select language. (Figure 3-7)
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3-7
Step 9、Click to select PCR program(Figure 3-8)
3-8
Step 10、Select installation path.(Figure 3-9)
3-9
Step 11、Confirm installation file name、create desk top icon、click to complete
installation.(Figure 3-10)
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3-10
3.2 Program upgrade
Step1. Double click master computer icon
interface. (Figure 3-11)
to enter program operation
3-11
Step2. While connect to Thermal Cycler, dialog box of update version will appear.
(Figure 3-12) Select”Yes”to start upgrading program.(Figure 3-13)
3-12
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3-13
Step3. Dialog box appears when upgrade completed, (Figure 3-14), click
“OK”and restart PCR, then upgrade completed.
3-14
3.3 Startup interface
After connecting related power, turn on the power switch of the instrument,it will
produces the sound of “tick-tack”, which means the instrument is in operation,.The
screen will display the startup interface (Figure 3-15), and start automatic systematic
inspection,After entering the stand-by interface (Figure 3-16),it means the instrument
is free for experimental operations now. If the screen is dark or any other abnormal
phenomena occur while turn on the instrument, please cut off the power immediately
and refer to Chapter 5 for maintenance or contact the distributor/the supplier for
settlement.
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3-15
3-16
3.4 Files
In the" Main Menu" select "File" submenu to get into "File" interface, choose the
"Open " or "Create ".(Figure 3-17)
3-17
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Notice: "Create” -- create new files.
"Open” -- open saved files.
Specific program file setting showed in chapter 4.
3.5 Setting
In the" Main Menu" select "Set" submenu to get into "Operation Parameters
Setting" interface. (Figure 3-18)
3-18
Notice: The Heating/Cooling rate range is 1.0℃/s~4.0℃/s. The temperature range of
hypothermic preservation can as low as 0.0℃.Temperature control mode:
sample block control and tube control.
3.6 Hot Lid
In the "Main Menu" select "Hot Lid" submenu to get into "Hot Lid Function Setting"
interface. (Figure 3-19)
3-19
Notice: the hot lid temperature range is: 20℃~110℃.
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3.7 Gradient
In the" Main Menu" select “Grads" submenu to get into the "Gradient View"
interface. (Figure 3-20)
3-20
Notice: Central temperature range: 31℃~99℃, grads range:1℃~15℃,the algebra
sum of the central temperature and the grads scale range:30 ℃ ~ 100
℃.Operator can view the temperature of 12 corresponding block rows.
The Gradient graph cannot used if user put in the 384module since the gradient
function of the 384module is not available.
3.8 Network
In the" Main Menu" select "Network" submenu to get into the "Network Setting"
interface. (Figure 3-21)
3-21
Notice: Network setting is mainly setting the IP address which set up network
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connection to the host computer, and the port number setting. Port number
8008 is a fixed value. IP address must be consistent with the host computer.
3.9 Log
In the "Main Menu" Select "Log" submenu to get into "Log View" interface. (Figure
3-22)
3-22
Notice: If the numbers of all currently created files is below 200, all the files’
information will be displayed in this window. If the number of files is above 200,
the most recently created 200 files’ information will be displayed. This
information include the file names, running time, and created date, etc.
3.10 System
In the" Main Menu" select "System" submenu to get into the "System Setting"
interface. (Figure 3-23)
3-23
Notice: Press the “Arrow” key to choose the system date, alert sound, file list
arrangement type, etc. for setting. System date and time setting should be
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inputted with the“Numerical”keys on the machine.
3.11 Help
In the "Main Menu" select "Help" submenu to get into "Help" interface. (Figure
3-24)
3-24
Notice: The “TM” key showed on the interface is for checking the TM value. If the
user inputs the value forbidden by the system while setting the parameter,
the system will choose the default value, and the forbidden value will not be
allowed. The version of this instrument is V2.0,showed in the“Help”
interface.
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Chapter4 Programming on Thermal Cycler
Notice: Select the “Close” key will cause the system be back to the Windows
CE system interface.
4.1 Create Files (The following pictures for reference only)
The following is a sample(30 cycles, 55℃:90s,0℃:30s,72℃:3mins) of programing.
(Figure 4-1)
“Close” key
Temp Edit Pull-down Menu
“Back” key
Cycle Edit Pull-down Menu
Right/Left selection key
Running Key
4-1
Step 1: Select the "Create" after entering the "File" menu, (Figure 4-2), then input the
file name (Figure 4-3).File name can be inputted with“Numerical”keys on the
machine or keypad in the interface. After that close the keypad and click the
"Confirm" key to confirm. After creating a new file you can choose to setting
password or not. If setting password, then click the "Confirm" key after input
password. (Figure 4-3)
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4-2
4-3
Step 2: Get into the "Temp Edit” and "Cycle Edit" interface for editing, click the "Temp
Edit" pull-down menu and choose the "Edit Temp Point" to edit temperature. If
you need to increase temperature, click the "Temp Edit" pull-down menu and
select "Add Temp Point" for editing. Select "Del. Temp Point” to remove temp
point if there is extra point. (Figure 4-4)
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4-4
Step 3: In the "Temp Edit”and" Cycle Edit" interface, use the“Right/Left selection”
key or“Arrow”keys to move the cursor to the temp point which will be the
temp cycle starting point, click the "Cycle Edit" and select the "Cycle Start
Point”. Then move to the one which will be the cycle ending point, click the
"Cycle Edit" and select the "Cycle End Point". (Figure 4-5)
4-5
Step 4: Click the "Run" key to start running files. (Figure4-6)
You can choose the “Run”,”Pause”,”Stop” keys to operate the program.
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4.2 Open Files
4-6
Click the "File" on the “main menu” interface to get into the "File” interface
(Figure 4-7), select "Open " to get into the file list interface(Figure4-8),then open the
file what you want to start running.
4-7
4-8
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Chapter5 Programming on Master Computer
Notice:Conect to Thermal Cycler with network cable.
1、Interface introduction
Double click master computer icon
to enter operation interface. (Figure
5-1) Interface is composed of menu bar, tool bar, PCR machine area, program area,
implementation view etc.
5-1
Key Name
Add PCR
Edit PCR
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Function
add PCR instrument
edit IP address of PCR(Not Port)
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Delete PCR
Connect
Disconnect
New
Edit
Delete
Run
Pause
Stop
delete PCR instrument
connect to Thermal Cycler
disconnect from Thermal Cycler
create PCR program
edit PCR program
delete PCR program
run PCR program
pause running PCR program
stop running PCR program
cancel operation
Cancel
Gradient table
view gradient table
TM calculation
TM
2、Create Files
Step 1: Click “Add”button and select PCR instrument. Input PCR instrument name, IP
address, IP port(fixed value, 8008), then click”OK”.(Figure 5-2)
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5-2
Step 2: click “Connect”button to connect to Thermal Cycler.
Step 3: click “New” button, dialog box of programming will appear. (Figure 5-3)
Users can edit program name, creater name and set password. System can provide
default program, however, users can edit via “program parameter”area at the left of
the interface according to the requirements. Then click “Save”button to save
program if confirmed.
5-3
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Step 4: Click “Run” button in the tool bar to run program. Double click”Running
view” to view the status of running program.(Figure 5-4)
5-4
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Appendix A Maintenance
and Troubleshooting
1、Maintenance
The instrument shall be stored in the dry and ventilating environment, keep away
from direct sunlight or humidity or shadiness.
Clean the wells of the block regularly in case the residue left by the experiment
affect the accuracy of the experiment, the corrosive solvent or cleaning agent is
forbidden, and cleaned by the cotton swab stained with water free alcohol in
appropriate amount is suggested, and then an compressed air is used to clean
each hole.
Cotton cloth or non-woven stained with appropriate pure water shall be used for
cleaning if there is dirt or dust on the surface of the instrument, organic solvent,
alcohol, strong acid, strong alkaline or other corrosive cleaning agent is not
allowed in case of any damage to the instrument surface.
After the experiment, check the instrument and turn off the power, remove all the
residues left by the experiment such as the water, reaction solution and impurity in
and out of the instrument to keep the instrument clean. The protective cover shall
be used to cover the instrument if it will not be used for a long time.
2、Troubleshooting
2.1 Bad experiment analysis
With the use of this instrument, if the experimental result is poor, then the
following problems maybe exist (Only for reference):
A. The reaction article is wrong, or the quantity is inadequate, or the purity is not
enough or the slight mononuclear chain area is incorrect.
B. The denatured temperature is too high or too low, so the user can add or reduce
time according to the reaction amount.
C. The annealing temperature is too high or too low.
“
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”
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D. The reactant concentration is too high or too low.
E. The preparation process is not specially treated.
F. The time and temperature value in the programming is not suitable.
G. The temperature of the sample or sample electrode is a little low, whereas the
base temperature is a little high.
H. The user shall check if the reaction tube is placed well, if not, little mineral oil
can be used to coat the cone hole to increase heat conduction.
I. Deviation may occur to temperature control if it is not used for a long time, if so
the user may require the manufacturer to readjust the temperature control,
any personal testing or readjustment of the temperature control is not
allowed.
2.2 Problem reason analysis and Corresponding
solving method
No.
1
Phenomenon
The screen is
dark and there
is no “tick”
sound after
opening the
power
2
The screen
displays wrong
box with the
sound of
“Tick…”
3
The slow temp
down speed of
the block or
failure of down
The slow temp
up speed of the
block or failure
of up
Failure of
heating or
cooling of the
block
Failure of
heating the heat
lid
The screen
displays
abnormal
character
4
5
6
7
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Analysis
The power is not connected
The fuse is burnout
The switch is damaged
others
Solutions
Connect the power
Change for a new
fuse 250V 10A
Ф5x20
Change the switch
Contact the supplier
or manufacturer
Contact the supplier
or manufacturer
(
)
1.The open circuit or short circuit
of the cooling block sensor
2 The open circuit or short circuit
of the heat lid sensor
3 The open circuit or short circuit
of the radiator
4 others
Block of vent
Remove the clogs
The cryogenic chip is damaged or Contact the supplier
the ventilator stop working.
or manufacturer
Cryogenic chip is damaged
Contact the supplier
or manufacturer
Temperature sensor is damaged
Cryogenic chip is damaged
Contact the supplier
or manufacturer
Heating film is damaged
Temperature sensor is damaged
The link line is loose
Bad contact of the chip
The chip is damaged
Contact the supplier
or manufacturer
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Contact the supplier
or manufacturer
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8
The press key
of the panel
does not
work
Button switch is damaged
Contact the supplier
or manufacturer
Appendix B Instrument Guarantee
1 Guarantee Content
a) The instrument, a common B-type instrument for indoor uses, is in accordance
with the I-type of the standards of GB9706.1, and has passed CE security
authentication.
b) Within one month as of the date of the delivery, for any damage caused by
materials or manufacturing happening to the instrument, we will change it..
c) Within 4 years as of the date of the delivery, for any damage caused by materials
or manufacturing happening to the instrument, our company will provide the
guarantee service. Within the warranty period, the company will repair or change
the instrument proved to be defective.
d) The user shall send the product to the maintenance department confirmed by our
company. The user shall pay the expense occurred by transferring the instrument
to the maintenance department. And the company shall pay the expense occurred
by transferring the instrument back to the user.
e) As to the repair beyond the warranty period, the company will charge the relevant
maintenance fees.
2 Guarantee Scope
a) Under normal working conditions, if any damage or default to the instrument is
caused by materials, installation or other flaws of the instrument itself, our
company shall provide the maintenance services according to the
above-mentioned maintenance contents.
b) If the user fails to operate according to the standard specified or uses it under the
inappropriate conditions, or arbitrarily dismantles or repairs the instrument
without authorization, then any damage or default thus occurred shall not be
contained in the maintenance scope.
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