SpectraMax Multi-Mode Microplate Readers Your applications, your modes, your choice > upgradeable platform for changing lab needs > three-mode cuvette port FOR ASSAY DEVELOPMENT > Dual monochromator tunability > AUTOMATED ABSORBANCE PATHLENGTH CORRECTION > Endpoint, kinetic, spectral and wellscanning read types > Comprehensive Data Analysis with SoftMax Pro Software > Validation & compliance > Robotics compatibility The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Software. Detection modes include: > UV-Visible Absorbance (Abs) > Fluorescence Intensity (FI) > Luminescence (Lum) > Time-Resolved Fluorescence (TRF) > Fluorescence Polarization (FP) The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® Assays. Dual Monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. Patented PATHLENGTH CORRECTION FOR BETTER ABSORBANCE ACCURACY Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no termperature dependency using the patented PathCheck® Sensor technology. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. This eliminates the need for standard curves, and, for compounds with known absorptive properties, enables users to calculate concentrations directly from absorbance. Superior Optics for Optimal Assay Performance Top Read Head From Excitation Monochromator FL, TRF, FP Excitation Reference Diode FL, TRF, FP, Lum Emission To Emission Monochromator and PMT Mirror Abs Beamsplitter and Excitation Polarizer Emission Mirror Emission Polarizers Absorbance Detector Diode Microplate Emission Mirror Beamsplitter Beamsplitter FL, TRF, FP Excitation Excitation Mirror From Excitation Monochromator FL, TRF, FP, Lum Emission Reference Diode Abs Bottom Read Head UNIQUE OPTICAL CHARAcTERISTICS Assay collaboration FOR EASE OF SETUP 1.The reference diodes enable elimination of measurement noise due to slight fluctuations in excitation light intensity. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. To support these assays, we provide application notes as well as ready-to-run protocols in our SoftMax Pro Software. Some of our featured partner assays include HTRF assays from Cisbio Bioassays and LanthaScreen® TR-FRET assays from Invitrogen. 2.The angled emission beam improves signalto-noise,especially in narrow Stokes shift fluorophores, by reducing stray light. 3.Elliptical mirrors are used instead of lenses for maximum transmission with minimal wavelength distortion. 4.Top-quality UV-grade fibers give the highest light transmission down to even the lowest wavelengths. Reference Diode From Excitation Monochromator IMAP PLATFORM ® VALIDATED To Emission Monochromator and PMT Which SpectraMax Microplate Reader Do You Need? SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification & Validation IMAP Validation (TR-FRET only) (TR-FRET/FP) (TR-FRET/FP) HTRF Certification LanthaScreen Certification Key Applications ELISAs DNA/RNA Quantitation Protein Assays Enzyme Kinetics Protease Assays Fluorescent Proteins and FRET Cell Viability and Cytotoxicity Assays Reporter Gene Assays Cell Migration Assays QBT Fatty Acid Uptake Assay Neurotransmitter Transporter Uptake Assay ADME-Tox Membrane Permeability Software for Data Aquisition and Analysis Powerful, Easy-to-Use Software SoftMax Pro Software features easy-to-set-up protocols for data acquisition, customizable spreadsheet functionality for analysis, and powerful graphing tools for data presentation. comprehensive data analysis and GxP solutions SoftMax Pro Software provides data acquisition, analysis and management capabilities, allowing cross-plate analysis and custom calculations. For users operating in a FDA 21 CFR Part 11 compliant environment, SoftMax Pro GxP Software is available, allowing user permissions, audit trails, e-signature and reporting tools. Tools for Validation and Compliance Validation Test Plates for Abs, FI, Lum Optical Performance SpectraTestABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e Microplate Readers. Validation and Compliance of Optical Characteristics SpectraMax Readers have the most complete level of product validation and compliance. Molecular Devices provides the complete solution covering the instrument and software: > SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes > IQ/OQ/PQ for all microplate readers > SoftMax Pro Software Validation Package > Software tools for FDA 21 CFR Part 11 compliance Automation Solutions StakMax Microplate Handling System Compatible Automation Solutions for SpectraMax Readers R Ion Potential ELISAs QA Testing Analysis DNA/RNA Purity Quantization & Normalization Solubility & Permeability Kinetic Assays Cytotoxicity Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. The system provides automation for up to 50 microplates for easy walkaway automation. System setup and calibration are controlled from within SoftMax Pro Software. SoftMax Pro 5 Software has been integrated by many leading robotics and LIMS providers, enabling both data analysis and instrument control in automated environments. Robotics Compatibility for increased throughput SpectraMax Multi-Mode Microplate Readers can be easily integrated with our optional StakMax® Microplate Handling System for walk-away processing. Operated from within SoftMax Pro Software, the StakMax System can hold up to 50 plates and facilitates barcode reading. For more advanced automation needs Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. Ordering Information Acquiring a SpectraMax Multi-Mode Microplate Reader is extremely easy: 1. Decide what modes you need and choose the specific configuration option that suits you best. All systems include SoftMax Pro Software for Windows® and Macintosh® Operating Systems. 2. Choose the additional options you want: > Software validation tools > SpectraTest ABS1, FL1, LM1 Validation Plates > SoftMax Pro GxP Software > StakMax Microplate Handing System 3. Contact your Molecular Devices sales representative to discuss the details. SpectraMax multi-mode microplate readers Technical Specifications General Specifications Dimensions (in.): 8.6 (H) x 22.8 (W) x 15.3 (D) Dimensions (cm): 22 (H) x 58 (W) x 39 (D) Weight: 36 lbs. (16.4 kg) Power consumption: < 420 watts Power source: 100–240 Vac, 3.5 A, 50/60 Hz Robotic-compatible:Yes General Photometric Performance Plate formats: 6, 12, 24, 48, 96, 384 wells Light source: Xenon Flash Lamp (1 joule/flash) Detectors: 2 photomultiplier tubes (PMT) Shaker time: 0 to 999 seconds Temp. control: 2°C above ambient to 60°C Temp. uniformity: < 1°C at 37°C set point Temp. accuracy: ±1°C at 37°C set point Endpoint reading : All modes Kinetic reading : All modes Spectral scanning: All modes Well scanning: Abs, FI, TRF, Lum Typical Read Times (minutes:seconds)* 96 wells 384 wells Absorbance 0:180:49 Fluorescence Intensity 0:17 0:48 Fluorescence Polarization 0:42 2:03 Time-Resolved Fluorescence 0:17 0:48 Luminescence 2:007:00 *With 3 flashes/well in absorbance and fluorescence modes, and 1 sec./ well integration in luminescence. Absorbance Photometric Performance Reading capabilities: Cuvette or microplate Wavelength range: 200–1000 nm Wavelength selection: Monochromator, tunable in 1.0 nm increments Wavelength bandwidth: ≤ 4.0 nm Wavelength accuracy: ±2.0 nm Wavelength repeatability: ±0.2 nm Photometric range: 0–4.0 OD Photometric resolution: 0.001 OD Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0–2 OD Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0–2 OD Photometric precision: < ±0.003 OD ±1.0%, 0–2 OD Stray light: < 0.05% @ 230 nm Fluorescence Intensity Performance Reading capabilities: Cuvette or top or bottom of a microplate Wavelength range: 250–850 nm Wavelength selection: Monochromators, tunable in 1.0 nm increments Bandwidth (EX, EM): 9 nm, 15 nm Sensitivity: < 5 pM fluorescein in 96 wells or cuvette, < 20 pM in 384 wells Luminescence Performance Reading capabilities: Cuvette or top or bottom of a microplate Wavelength selection: Choice of simultaneous detection of all wavelengths or selection via monochromator, tunable in 1.0 nm increments Wavelength range: 250–850 nm Sensitivity: < 2 fg/well lower detection limit for firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0.3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities: Top or bottom of a microplate Wavelength range: 250–850 nm Wavelength selection: Monochromators, tunable in 1.0 nm increments Bandwidth (EX, EM): 9 nm, 15 nm Precision data collection: 1–100 flashes, delay of 0–600 µsec. before read, integration time selectable between 50–1500 µsec. Sensitivity: 100 fM europium in 96 or 384 wells with top-read SpectraMax M5e reader only: Certified to Cisbio Bioassays’ HTRF assays performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range: 300–750 nm Wavelength selection: Monochromators, tunable in 1.0 nm increments Bandwidth (EX, EM): 9 nm, 15 nm Precision: < 5 mP standard deviation at 1 nM fluorescein in 96 and 384 wells Patents The PathCheck Sensor is covered under U.S. Patents 5,959,738, 6,188,476, 6,320,662, 6,339,472, 6,404,501, 6,496,260 and 6,995,844. SpectraMax M3, M4, M5, and M5e readers are also covered under U.S. Patents 6,097,025 6,232,608, 6,236,456, 6,313,471 and 6,316,774 6,693,709, and 6,825,921. Sales OFFICES United States and Canada Molecular Devices Tel. +1-800-635-5577 Fax +1-408-747-3601 China Molecular Devices Beijing Tel. +86-10-6410-8669 Fax +86-10-6410-8601 Japan Molecular Devices Japan, Osaka Tel. +81-6-6399-8211 Fax +81-6-6399-8212 United Kingdom Molecular Devices (GB) Ltd. Tel. +44-118-944-8000 Fax +44-118-944-8001 Brazil Molecular Devices Brazil Tel. +55-11-3616-6607 Fax +55-11-3616-6607 Molecular Devices Shanghai Tel. +86-21-3372-1088 Fax +86-21-3372-1066 Molecular Devices Japan, Tokyo Tel. +81-3-5282-5261 Fax +81-3-5282-5262 www.moleculardevices.com Germany Molecular Devices GmbH Tel. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 South Korea Molecular Devices Korea, LLC Tel. +82-2-3471-9531 Fax +82-2-3471-9532 The trademarks used herein are the property of ©2011 Molecular Devices, Inc. Printed in U.S.A. 6/11 #0120-1498D FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Molecular Devices, Inc. or their respective owners. Specifications subject to change without notice.
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