SpectraMax Multi-Mode Microplate Readers Literature

SpectraMax Multi-Mode Microplate Readers Literature
SpectraMax Multi-Mode Microplate Readers
Your applications, your modes, your choice
> upgradeable platform
for changing lab needs
> three-mode cuvette port
FOR ASSAY DEVELOPMENT
> Dual monochromator
tunability
> AUTOMATED ABSORBANCE
PATHLENGTH CORRECTION
> Endpoint, kinetic,
spectral and wellscanning read types
> Comprehensive Data
Analysis with SoftMax
Pro Software
> Validation & compliance
> Robotics compatibility
The SpectraMax® M3, M4, M5 and M5e
Multi-Mode Microplate Readers are a modular,
upgradeable dual-monochromator microplate
reader platform offering a wide range of high
performance multi-mode capabilities ideal for life
science research and drug discovery screening.
Choose from a three- (M3), four- (M4), or
five- (M5/M5e) mode reader customized to
your specific applications or budgetary needs,
while optional capabilities allow you to upgrade
with other detection modes at a later time. All
configurations offer a triple-mode cuvette port,
accurate temperature control, microplate shaking
and comprehensive data management using
our SoftMax® Pro Software. Detection modes
include:
>
UV-Visible Absorbance (Abs)
>
Fluorescence Intensity (FI)
>
Luminescence (Lum)
>
Time-Resolved Fluorescence (TRF)
>
Fluorescence Polarization (FP)
The SpectraMax M5e Reader offers the additional
benefit of being certified for Cisbio Bioassays’
HTRF® Assays.
Dual Monochromators for assay flexibility
With SpectraMax Multi-Mode Readers, there is
no need to utilize expensive filters to optimize
detection levels and background. The optical
systems use two scanning monochromators so
the user can determine optimal excitation and
emission settings, resulting in assay performance
similar to that of dedicated single-mode readers.
Patented PATHLENGTH CORRECTION FOR
BETTER ABSORBANCE ACCURACY
Only Molecular Devices microplate plate readers
offer the capability to measure the depth (optical
pathlength) of samples with no termperature
dependency using the patented PathCheck®
Sensor technology. With SoftMax Pro Software,
the PathCheck Sensor automatically normalizes
the well absorbance. This eliminates the need for
standard curves, and, for compounds with known
absorptive properties, enables users to calculate
concentrations directly from absorbance.
Superior Optics for Optimal Assay Performance
Top Read Head
From Excitation
Monochromator
FL, TRF, FP Excitation
Reference Diode
FL, TRF, FP, Lum Emission
To Emission
Monochromator
and PMT
Mirror
Abs
Beamsplitter and
Excitation Polarizer
Emission Mirror
Emission Polarizers
Absorbance Detector Diode
Microplate
Emission Mirror
Beamsplitter
Beamsplitter
FL, TRF, FP Excitation
Excitation Mirror
From
Excitation
Monochromator
FL, TRF, FP, Lum Emission
Reference Diode
Abs
Bottom Read Head
UNIQUE OPTICAL CHARAcTERISTICS
Assay collaboration FOR EASE OF SETUP
1.The reference diodes enable elimination of
measurement noise due to slight fluctuations
in excitation light intensity.
Molecular Devices has collaborated with
various assay partners to optimize and
validate homogeneous and heterogeneous
biochemical- or cell-based assay performance
on the SpectraMax platform. To support these
assays, we provide application notes as well
as ready-to-run protocols in our SoftMax Pro
Software. Some of our featured partner assays
include HTRF assays from Cisbio Bioassays
and LanthaScreen® TR-FRET assays from
Invitrogen.
2.The angled emission beam improves signalto-noise,especially in narrow Stokes shift
fluorophores, by reducing stray light.
3.Elliptical mirrors are used instead of lenses
for maximum transmission with minimal
wavelength distortion.
4.Top-quality UV-grade fibers give the highest
light transmission down to even the lowest
wavelengths.
Reference Diode
From Excitation
Monochromator
IMAP
PLATFORM
®
VALIDATED
To Emission
Monochromator
and PMT
Which SpectraMax Microplate Reader Do You Need?
SpectraMax M3
Reader
SpectraMax M4
Reader
SpectraMax M5
Reader
SpectraMax M5e
Reader
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TRF,HTRF,FP
HTRF, FP
HTRF
N/A
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Detection Modes
Absorbance
Fluorescence Intensity
Luminescence
Time-Resolved Fluorescence
Fluorescence Polarization
HTRF
Upgrade Options
Plate Formats
6-, 12-, 24-, 48-, 96-, 384-Well Microplates
Certification & Validation
IMAP Validation
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(TR-FRET only)
(TR-FRET/FP)
(TR-FRET/FP)
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HTRF Certification
LanthaScreen Certification
Key Applications
ELISAs
DNA/RNA Quantitation
Protein Assays
Enzyme Kinetics
Protease Assays
Fluorescent Proteins and FRET
Cell Viability and Cytotoxicity Assays
Reporter Gene Assays
Cell Migration Assays
QBT Fatty Acid Uptake Assay
Neurotransmitter Transporter Uptake Assay
ADME-Tox
Membrane Permeability
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Software for Data Aquisition and Analysis
Powerful, Easy-to-Use Software
SoftMax Pro Software features easy-to-set-up protocols for data acquisition, customizable
spreadsheet functionality for analysis, and powerful graphing tools for data presentation.
comprehensive data analysis and GxP solutions
SoftMax Pro Software provides data acquisition, analysis and
management capabilities, allowing cross-plate analysis and
custom calculations. For users operating in a FDA 21 CFR
Part 11 compliant environment, SoftMax Pro GxP Software
is available, allowing user permissions, audit trails, e-signature
and reporting tools.
Tools for Validation and Compliance
Validation Test Plates for Abs, FI, Lum Optical Performance
SpectraTest­ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1
Luminescence Plates are used to validate optical performance of SpectraMax
M3, M4, and M5/M5e Microplate Readers.
Validation and Compliance of Optical
Characteristics
SpectraMax Readers have the most complete level of
product validation and compliance. Molecular Devices
provides the complete solution covering the instrument
and software:
> SpectraTest® ABS1, FL1, and LM1 Validation Plates
for hardware validation of absorbance, fluorescence,
and luminescence modes
>
IQ/OQ/PQ for all microplate readers
>
SoftMax Pro Software Validation Package
>
Software tools for FDA 21 CFR Part 11 compliance
Automation Solutions
StakMax Microplate Handling System
Compatible Automation Solutions for SpectraMax Readers
R
Ion Potential
ELISAs
QA Testing
Analysis
DNA/RNA
Purity
Quantization &
Normalization
Solubility &
Permeability
Kinetic Assays
Cytotoxicity
Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate
Handling System from Molecular Devices. The system provides automation
for up to 50 microplates for easy walkaway automation. System setup and
calibration are controlled from within SoftMax Pro Software.
SoftMax Pro 5 Software has been integrated by many leading robotics and LIMS providers, enabling
both data analysis and instrument control in automated environments.
Robotics Compatibility for increased
throughput
SpectraMax Multi-Mode Microplate Readers can
be easily integrated with our optional StakMax®
Microplate Handling System for walk-away
processing. Operated from within SoftMax Pro
Software, the StakMax System can hold up to 50
plates and facilitates barcode reading.
For more advanced automation needs Molecular
Devices interacts with all of the major lab
automation providers, and is one of their leading
choices.
Ordering Information
Acquiring a SpectraMax Multi-Mode Microplate
Reader is extremely easy:
1. Decide what modes you need and choose the
specific configuration option that suits you best.
All systems include SoftMax Pro Software for
Windows® and Macintosh® Operating Systems.
2. Choose the additional options you want:
> Software validation tools
> SpectraTest ABS1, FL1, LM1 Validation Plates
> SoftMax Pro GxP Software
> StakMax Microplate Handing System
3. Contact your Molecular Devices sales
representative to discuss the details.
SpectraMax multi-mode microplate readers
Technical Specifications
General Specifications
Dimensions (in.): 8.6 (H) x 22.8 (W) x 15.3 (D)
Dimensions (cm): 22 (H) x 58 (W) x 39 (D)
Weight:
36 lbs. (16.4 kg)
Power consumption: < 420 watts
Power source:
100–240 Vac, 3.5 A, 50/60 Hz
Robotic-compatible:Yes
General Photometric Performance
Plate formats:
6, 12, 24, 48, 96, 384 wells
Light source:
Xenon Flash Lamp
(1 joule/flash)
Detectors:
2 photomultiplier tubes (PMT)
Shaker time:
0 to 999 seconds
Temp. control:
2°C above ambient to 60°C
Temp. uniformity: < 1°C at 37°C set point
Temp. accuracy: ±1°C at 37°C set point
Endpoint reading : All modes
Kinetic reading : All modes
Spectral scanning: All modes
Well scanning:
Abs, FI, TRF, Lum
Typical Read Times (minutes:seconds)*
96 wells 384 wells
Absorbance
0:180:49
Fluorescence Intensity
0:17
0:48
Fluorescence Polarization
0:42
2:03
Time-Resolved Fluorescence 0:17
0:48
Luminescence
2:007:00
*With 3 flashes/well in absorbance and fluorescence modes, and 1 sec./
well integration in luminescence.
Absorbance Photometric Performance
Reading capabilities: Cuvette or microplate
Wavelength range:
200–1000 nm
Wavelength selection: Monochromator, tunable
in 1.0 nm increments
Wavelength bandwidth: ≤ 4.0 nm
Wavelength accuracy: ±2.0 nm
Wavelength repeatability: ±0.2 nm
Photometric range:
0–4.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate):
< ±0.006 OD ±1.0%, 0–2 OD
Photometric accuracy (cuvette):
< ±0.005 OD ±1.0%, 0–2 OD
Photometric precision:
< ±0.003 OD ±1.0%, 0–2 OD
Stray light:
< 0.05% @ 230 nm
Fluorescence Intensity Performance
Reading capabilities: Cuvette or top or
bottom of a microplate
Wavelength range:
250–850 nm
Wavelength selection: Monochromators, tunable
in 1.0 nm increments
Bandwidth (EX, EM): 9 nm, 15 nm
Sensitivity: < 5 pM fluorescein in 96 wells or
cuvette, < 20 pM in 384 wells
Luminescence Performance
Reading capabilities: Cuvette or top or bottom
of a microplate
Wavelength selection: Choice of simultaneous
detection of all wavelengths or selection via
monochromator, tunable in 1.0 nm increments
Wavelength range:
250–850 nm
Sensitivity: < 2 fg/well lower detection limit for
firefly luciferase in 96- and 384-well top read
Dynamic range:
> 6 decades
Cross-talk:
< 0.3% in white 96- and
384-well microplates
Time-Resolved Fluorescence Performance
(M4, M5, M5e only)
Reading capabilities: Top or bottom of a
microplate
Wavelength range:
250–850 nm
Wavelength selection: Monochromators, tunable
in 1.0 nm increments
Bandwidth (EX, EM): 9 nm, 15 nm
Precision data collection: 1–100 flashes, delay
of 0–600 µsec. before read, integration time
selectable between 50–1500 µsec.
Sensitivity: 100 fM europium in 96 or 384 wells
with top-read
SpectraMax M5e reader only: Certified to
Cisbio Bioassays’ HTRF assays performance
specifications
Fluorescence Polarization Performance
(M5/M5e only)
Wavelength range:
300–750 nm
Wavelength selection: Monochromators, tunable
in 1.0 nm increments
Bandwidth (EX, EM): 9 nm, 15 nm
Precision: < 5 mP standard deviation at 1 nM
fluorescein in 96 and 384 wells
Patents
The PathCheck Sensor is covered under U.S.
Patents 5,959,738, 6,188,476, 6,320,662,
6,339,472, 6,404,501, 6,496,260 and 6,995,844.
SpectraMax M3, M4, M5, and M5e readers
are also covered under U.S. Patents 6,097,025
6,232,608, 6,236,456, 6,313,471 and 6,316,774
6,693,709, and 6,825,921.
Sales OFFICES
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Molecular Devices
Tel. +1-800-635-5577
Fax +1-408-747-3601
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Molecular Devices Beijing
Tel. +86-10-6410-8669
Fax +86-10-6410-8601
Japan
Molecular Devices Japan, Osaka
Tel. +81-6-6399-8211
Fax +81-6-6399-8212
United Kingdom
Molecular Devices (GB) Ltd.
Tel. +44-118-944-8000
Fax +44-118-944-8001
Brazil
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Tel. +55-11-3616-6607
Fax +55-11-3616-6607
Molecular Devices Shanghai
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Tel. +81-3-5282-5261
Fax +81-3-5282-5262
www.moleculardevices.com
Germany
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The trademarks used herein are the property of
©2011 Molecular Devices, Inc. Printed in U.S.A. 6/11 #0120-1498D
FOR RESEARCH USE ONLY. NOT FOR USE IN
DIAGNOSTIC PROCEDURES.
Molecular Devices, Inc. or their respective owners.
Specifications subject to change without notice.
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