Accela UHPLC System Getting Connected Guide Rev.F

Accela UHPLC System Getting Connected Guide Rev.F
Accela UHPLC System
Getting Connected Guide
60057-97001 Revision F
March 2011
© 2011 Thermo Fisher Scientific Inc. All rights reserved.
Exactive, LCQ, LightPipe, MSQ Plus, TSQ, and Velos are trademarks and Accela, ChromQuest, Xcalibur,
LTQ, and LXQ are registered trademarks of Thermo Fisher Scientific Inc. in the United States.
The following are registered trademarks in the United States and other countries: Fluorinert is a registered
trademark of 3M Corporation. Fomblin is a registered trademark of Solvay Solexis S.p.A. Teflon and Freon are
registered trademarks of E.I. du Pont de Nemours and Company. Rheodyne is a registered trademark of IDEX
Health & Science, LLC. Valco and Cheminert are registered trademarks of Valco Instruments Co. Inc. and
VICI AG.
The following are trademarks in the United States and other countries: PEEK is a trademark of Victrex PLC.
Super Flangeless is a trademark of IDEX Health & Science, LLC.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or errorfree and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history: Revision A, September 2006; Revision B, March 2008; Revision C, February 2009;
Revision D, October 2009; Revision E, March 2010; Revision F, March 2011
Revision F includes information about the latest addition to the Accela product line, the Accela Open
Autosampler.
For Research Use Only. Not for use in diagnostic procedures.
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent
electromagnetic compatibility (EMC) and safety standards as described in the next section or sections by product name.
Changes that you make to your system may void compliance with one or more of these EMC and safety standards.
Changes to your system include replacing a part or adding components, options, or peripherals not specifically
authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards,
replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific
or one of its authorized representatives.
Accela Pump and Accela Autosampler
EMC Directive 89/336/EEC, 92/31/EEC, 93/68/EEC
EMC compliance has been evaluated by TUV Rheinland of North America, Inc.
EN 61326: 1997; A1, 1998; A2, 2001; A3, 2003
EN 61000-4-4: 1995; A1, 2000; A2, 2001
EN 61000-3-2: 2000
EN 61000-4-5: 2001
EN 61000-3-3: 1995; A1, 2001
EN 61000-4-6: 2003
EN 61000-4-2: 2001
EN 61000-4-8: 2001
EN 61000-4-3: 2002
EN 61000-4-11: 2001
FCC Class A, CFR 47 Part 15 Subpart B: 2005
Low Voltage Safety Compliance
Low Voltage Safety Compliance has been evaluated by TUV Rheinland of North America, Inc.
This device complies with Low Voltage Directive 73/23/EEC and harmonized standard EN 61010-1:2001,
IEC 61010-1:2002, UL 61010 A-1:2004, CAN/CSA 22.2 61010-1:2004.
Accela Open Autosampler
EMC Directive 2004/108/EC
EMC compliance has been evaluated by CTC Analytics for the HTC PAL™ autosampler.
IEC 61326-1:2005
IEC 61326-2-6: 2005
EN 61326-1: 1997, A1:1998
CISPR 22:2005, A1:2005, A2:2006
FCC Class A, CFR 47 Part 15: 2003
Low Voltage Safety Compliance
Low Voltage Safety Compliance has been evaluated by CTC Analytics for the HTC PAL autosampler.
This device complies with Low Voltage Directive 2006/95/EC and harmonized standard EN 61010-1:2001,
IEC 61010-1:2001, ANSI/UL 61010 A-1:2004, CAN/CSA 22.2 61010-1:2004.
Accela 600 Pump
EMC Directive 2004/108/EC
EMC compliance has been evaluated by TUV Rheinland of North America Inc.
EN 61326-1: 2006
EN 61000-4-3: 2006
EN 55011: 2007
EN61000-4-4: 2004
EN 61000-3-2: 2006
EN61000-4-5: 2005
EN 61000-3-3: 1995, A1: 2001, A2: 2005
EN61000-4-6: 2007
EN 61000-4-2: 1995, A1: 1999, A2: 2001
EN61000-4-11: 2004
FCC Class A: CFR 42, Part 15: 2007
Low Voltage Safety Compliance
This device complies with Low Voltage Directive 2006/95/EC and the following harmonized standards:
EN 61010-1: 2001, IEC 61010-1: 2002, UL 61010A-1: 2004, CAN/CSA 22.2 61010-1: 2004.
Accela 1250 Pump
EMC Directive 2004/108/EC
EMC compliance has been evaluated by TUV Rheinland of North America Inc.
EN 55011: 2007
EN 61000-4-3: 2006
EN 61000-3-2: 2006
EN 61000-4-4: 2006
EN 61000-3-3: 1995, A1: 2001, A2: 2005
EN 61000-4-5: 2005
EN 61000-4-2: 1995, A1: 1999, A2: 2001
EN 61000-4-6: 2007
EN 61326-1: 2006
EN 61000-4-11: 2004
FCC Class A: CFR 47, Part 15: 2009
Low Voltage Safety Compliance
This device complies with Low Voltage Directive 2006/95/EC and the following harmonized standards:
EN 61010-1: 2001, IEC 61010-1: 2002, UL 61010A-1: 2004, CAN/CSA 22.2 61010-1: 2004.
Accela PDA Detector
EMC Directive 89/336/EEC, 92/31/EEC, 93/68/EEC
EMC compliance has been evaluated by TUV Rheinland of North America, Inc.
EN 61326:1997; A1, 1998; A2, 2001; A3, 2003
EN 61000-4-4: 1995; A1, 2000; A2, 2001
EN 61000-3-2: 2000
EN 61000-4-5: 2001
EN 61000-3-3: 1995; A1, 2001
EN 61000-4-6: 2003
EN 61000-4-2: 2001
EN 61000-4-8: 2001
EN 61000-4-3: 2002
EN 61000-4-11: 2001
FCC Class A, CFR 47 Part 15 Subpart B: 2005
Low Voltage Safety Compliance
Low Voltage Safety Compliance has been evaluated by TUV Rheinland of North America, Inc.
This device complies with Low Voltage Directive 73/23/EEC and harmonized standard EN 61010-1:2001,
IEC 61010-1:2002, UL 61010 A-1:2004, CAN/CSA 22.2 61010-1:2004.
Accela PDA Detector (80 Hz version)
EMC Directive 2004/108/EC
EMC compliance has been evaluated by TUV Rheinland of North America, Inc.
EN 61326-1: 2006
EN 61000-4-3: 2006
EN 55011: 2007, A2: 2007
EN 61000-4-4: 2004
EN 61000-3-2: 2006
EN 61000-4-5: 2005
EN 61000-3-3: 1995, A1: 2001, A2: 2005
EN 61000-4-6: 2007
EN 61000-4-2: 1995, A1: 1999, A2: 2001
EN 61000-4-11: 2001
FCC Class A, CFR 47 Part 15: 2007
Low Voltage Safety Compliance
Low Voltage Safety Compliance has been evaluated by TUV Rheinland of North America, Inc.
This device complies with Low Voltage Directive 2006/95/EC and harmonized standard EN 61010-1:2001,
IEC 61010-1:2002, UL 61010 A-1:2004, CAN/CSA 22.2 61010-1:2004.
Accela RI Detector
EMC Directive 2004/108/EC
EMC compliance has been evaluated by TÜV Rheinland of North America, Inc.
EN 55011: 2007
EN 61000-4-3: 2006
EN 61000-3-2: 2006
EN 61000-4-4: 2004
EN 61000-3-3: 1995, A1; 2001, A2; 2005
EN 61000-4-5: 2005
EN 61326-1: 2006
EN 61000-4-6: 2007
EN 61000-4-2: 1995, A1; 1999, A2; 2001
EN 61000-4-11: 2004
FCC Class A, CFR 47 Part 15: 2009
Low Voltage Safety Compliance
Low Voltage Safety Compliance has been evaluated by TUV Rheinland of North America, Inc.
This device complies with Low Voltage Directive 2006/95/EC and harmonized standard EN 61010-1:2001,
IEC 61010-1:2002, UL 61010 A-1:2004, CAN/CSA 22.2 61010-1:2004.
Accela UV/Vis Detector
EMC Directive 2004/108/EC
EMC compliance has been evaluated by TÜV Rheinland of North America, Inc.
EN 55011: 2007
EN 61000-4-3: 2006
EN 61000-3-2: 2006
EN 61000-4-4: 2004
EN 61000-3-3: 1995, A1; 2001, A2; 2005
EN 61000-4-5: 2005
EN 61326-1: 2006
EN 61000-4-6: 2007
EN 61000-4-2: 1995, A1; 1999, A2; 2001
EN 61000-4-11: 2004
FCC Class A, CFR 47 Part 15: 2008
Low Voltage Safety Compliance
Low Voltage Safety Compliance has been evaluated by TUV Rheinland of North America, Inc.
This device complies with Low Voltage Directive 2006/95/EC and harmonized standard EN 61010-1:2001,
IEC 61010-1:2002, UL 61010 A-1:2004, CAN/CSA 22.2 61010-1:2004.
FCC Compliance Statement
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside
this manual pertaining to the safe use and operation of this product before using the device.
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific
instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one
person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency
transmitters, such as mobile phones, in close proximity to the instrument.
For manufacturing location, see the label on the instrument.
WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union
(EU) Member State, and these companies should dispose of or recycle this product. See www.thermo.com/
WEEERoHS for further information on Thermo Fisher Scientific’s compliance with these Directives and the
recyclers in your country.
WEEE Konformität
Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC erfüllen.
Das Produkt ist durch folgendes Symbol gekennzeichnet:
Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten
getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information
über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise,
die nützlich sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter
www.thermo.com/WEEERoHS.
Conformité DEEE
Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et
Electroniques (DEEE). Il est marqué par le symbole suivant:
Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de
l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage d'informations sur la
conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre pays et les informations sur les
produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont
disponibles sur www.thermo.com/WEEERoHS.
C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Thermo Scientific
Chapter 1
Introduction to the Accela LC System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Lifting and Carrying the Accela System Modules. . . . . . . . . . . . . . . . . . . . . . . . . 4
Accela Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Accela Open Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Chapter 2
Back Panel Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
System Synchronization Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Accela Autosampler System Synchronization Connections . . . . . . . . . . . . . . . 8
Accela Open Autosampler System Synchronization Connections . . . . . . . . . 14
Communication Connections to the Data System Computer . . . . . . . . . . . . . . 24
Connecting the Accela Autosampler and Detector to the Data System
Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Connecting the Pump to the Data System Computer . . . . . . . . . . . . . . . . . . 27
Connecting the Accela Open Autosampler to the Data System
Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Line Power Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Connecting the Accela Autosampler, Detector, and Pump to Line
Power. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Connecting the Accela Open Autosampler to Line Power . . . . . . . . . . . . . . . 31
Chapter 3
Solvent Line Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Routing the Tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Connecting the Wash Bottle Tubing to the Syringe Valve. . . . . . . . . . . . . . . . . 37
Connecting the Needle Tubing to the Syringe Valve . . . . . . . . . . . . . . . . . . . . . 38
Low-Pressure Solvent Reservoir Line Connections. . . . . . . . . . . . . . . . . . . . . . . 38
Connecting the Solvent Reservoir Lines to the Accela Pump . . . . . . . . . . . . . 39
Connecting the Solvent Reservoir Lines to the Accela 600 or Accela
1250 Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Accela LC System Getting Connected Guide
xi
Contents
High-Pressure Connection between the Pump and the Autosampler . . . . . . . . . 45
Connecting the High-Pressure Solvent Line to the Pump Outlet. . . . . . . . . . 45
Connecting the High-Pressure Solvent Line to the Autosampler . . . . . . . . . . 47
LC Column Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Connecting the LC Column Inlet to the Autosampler Injection Valve . . . . . 50
Connecting the LC Column Outlet to the Detector Inlet . . . . . . . . . . . . . . . 52
Mounting the LC Column to the Column Support . . . . . . . . . . . . . . . . . . . 52
Connecting the RI Detector Outlet to Waste . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Chapter 4
Drainage Tubing Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Accela Autosampler and Accela Pump Drainage Connections . . . . . . . . . . . . . . 57
Accela Open Autosampler Drainage Connections . . . . . . . . . . . . . . . . . . . . . . . 61
Appendix A System Connection Parts and Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63
Accela System Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Solvent Interconnect Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Accela 600 and Accela 1250 Pump Inlet Tubing Kit . . . . . . . . . . . . . . . . . . . . . 65
Accela Open Autosampler Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
xii
Accela LC System Getting Connected Guide
Thermo Scientific
P
Preface
This guide describes how to set up and interconnect the modules of the Accela™ LC system
and how to make contact closure between the LC system and a Thermo Scientific MS
detector.
Related Documentation
In addition to this guide, Thermo Fisher Scientific provides the following documents as PDF
files for the Accela system:
• Accela Preinstallation Requirements Guide
• Accela Autosampler Hardware Manual
• Accela Open Autosampler Hardware Manual
• Accela Open Autosampler User Guide
• Accela Pump Hardware Manual
• Accela 600 Pump and Accela 1250 Pump Hardware Manual
• Accela PDA Detector Hardware Manual
• Accela PDA Detector (80 Hz Version) Hardware Manual
• Accela UV/Vis Detector Hardware Manual
• Accela RI Detector Hardware Manual
• Accela user guide for each data system
The data system also provides Help.
Thermo Scientific
Accela LC System Getting Connected Guide
xiii
Preface
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.
CAUTION Highlights information necessary to prevent personal injury caused by
touching a hot surface.
LIFTING HAZARD Highlights information necessary to prevent personal injury caused by
incorrect lifting of a heavy object.
CAUTION Highlights information necessary to prevent personal injury caused by
potential chemical hazards.
CAUTION Highlights potential fire hazards.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Highlights information of general interest.
Tip Highlights helpful information that can make a task easier.
xiv
Accela LC System Getting Connected Guide
Thermo Scientific
Preface
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
 To contact Technical Support
Phone
800-532-4752
Fax
561-688-8736
E-mail
[email protected]
Knowledge base
www.thermokb.com
Find software updates and utilities to download at mssupport.thermo.com.
 To contact Customer Service for ordering information
Phone
800-532-4752
Fax
561-688-8731
E-mail
[email protected]
Web site
www.thermo.com/ms
 To get local contact information for sales or service
Go to www.thermoscientific.com/wps/portal/ts/contactus.
 To copy manuals from the Internet
Go to mssupport.thermo.com, agree to the Terms and Conditions, and then click
Customer Manuals in the left margin of the window.
 To suggest changes to documentation or to Help
• Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62.
• Send an e-mail message to the Technical Publications Editor at
[email protected]
Thermo Scientific
Accela LC System Getting Connected Guide
xv
1
Introduction to the Accela LC System
The Accela ultra-high-performance liquid chromatography (UHPLC) system (see Figure 1)
consists of an analytical pump, an autosampler, and an optional photodiode array (PDA)
detector, ultra-violet/visible (UV/Vis) detector, or refractive index (RI) detector. You can
control these devices from a Thermo Scientific data system.
Contents
• Overview
• Lifting and Carrying the Accela System Modules
Figure 1.
Accela LC systems with an Accela Autosampler or an Accela Open Autosampler
Accela Open
Autosampler
Accela solvent
platform
Accela PDA Detector,
Accela UV/Vis Detector,
or Accela RI Detector
Accela
Autosampler
Accela Pump,
Accela 600 Pump, or
Accela 1250 Pump
Thermo Scientific
Autosampler
table
Accela LC System Getting Connected Guide
1
1 Introduction to the Accela LC System
Overview
Overview
The system layout depends on the autosampler model. For systems that include an Accela
Autosampler, the autosampler sits above the analytical pump, followed by the detector. The
Accela solvent platform, containing the solvent reservoir bottles, is located on the top of the
stack. For systems that include an Accela Open Autosampler, the autosampler is installed on
its own table. The analytical pump and detector stack fit below the table (see Figure 1 on
page 1).
Four 1/8 in. OD, fluorinated ethylene propylene (FEP) solvent lines carry solvent from the
reservoir bottles down to the vacuum membrane degasser built into an Accela pump. Four
high-precision solenoid valves in the pump proportion the solvents to create the requested
mobile phase composition. If the pump has the optional dynamic mixer installed, the liquid
displacement assembly (LDA) delivers mobile phase to the inlet port of the dynamic mixer. As
the mixed mobile phase exits the mixer, it flows into the autosampler heat exchanger where it
equilibrates to the temperature specified in the chromatographic method before it reaches the
autosampler injection valve.
Note Accela pumps shipped before March 2008 do not have a built-in dynamic mixer.
Call your local Thermo Fisher Scientific field service representative for information on
upgrading these pumps. Adding the dynamic mixer to the Accela Pump ensures mixing of
complex or relatively incompatible solvents and reduces the chromatographic baseline
noise for applications using proportioned mobile phases. The integrated dynamic mixer
also increases the accuracy and reproducibility of complex ternary and quaternary
gradients. The dynamic mixer adds 35 μl of gradient delay volume to the system.
The Accela 600 and Accela 1250 pumps ship with a placeholder module that connects the
flow path between the liquid displacement assembly and the autosampler. The placeholder
module is a block of stainless steel with an internal bore for the solvent flowpath and inlet
and outlet ports. The dynamic mixer is an add-on hardware option that provides solvent
mixing and physically replaces the placeholder module.
The Accela autosampler draws a sample from a sample vial or well and meters it into an
isolated sample loop attached to the injection valve. The injection valve switches to the inject
position, which places the sample loop in line between the pump and column. Mobile phase
backflushes the sample from the sample loop into the LC column.
As compounds elute from the LC column, they pass through a short length of tubing into the
detector’s flow cell. In the Accela UV/Vis and Accela PDA detectors, each component in turn
absorbs light according to its molar absorptivity and concentration. The detectors measure the
reduction in light intensity due to the absorbance of each component, and the data system
reports the absorbance as a function of wavelength and time.
In the Accela RI Detector, which is a differential refractive index detector, eluting compounds
pass through the sample compartment of the detector’s flow cell. The RI detector measures
the deflection of a light beam caused by the refractive index difference between the contents of
the sample compartment and that of the reference compartment, which contains mobile
phase. The data system reports the signal in refractive index units (RIU) as a function of time.
2
Accela LC System Getting Connected Guide
Thermo Scientific
1
Introduction to the Accela LC System
Overview
CAUTION The pressure rating for the flow cell of the Accela RI Detector is 517 kPA
(75 psi). When you connect two detectors in series, place the refractive index detector at
the end of the solvent path.
When the Accela system is connected to a mass spectrometer, the liquid-phase mixture of
analyte components and mobile phase exits the LC column and travels through another
length of 0.005 in. ID, red tubing (supplied in the accessory kit for the MS detector) to the
mass spectrometer. The mass spectrometer’s ion source nebulizes and ionizes the liquid-phase
mixture, its ion optics transmit the ions into the mass analyzer, and the mass analyzer
separates the ions according to their mass-to-charge ratios (m/z). As the ions sequentially exit
the mass analyzer, they impinge upon a conversion dynode/electron multiplier detector,
which sends a signal to the data system computer. The data system reports the ion abundance
as a function of the m/z ratio of the ions.
Thermo Scientific
Accela LC System Getting Connected Guide
3
1 Introduction to the Accela LC System
Lifting and Carrying the Accela System Modules
Lifting and Carrying the Accela System Modules
Because each Accela module weighs more than 18 kg (40 lb), attempting to lift any of these
modules without assistance can cause personal injury. In accordance with international safety
regulations, lifting or moving objects heavier than 18 kg (40 lb) requires a team effort.
CAUTION Because each Accela module weighs more than 18 kg (40 lb), in accordance
with international safety regulations, lifting or moving any of these modules requires a
team effort.
The autosamplers are the largest and heaviest modules in the Accela system.
Accela Autosampler
The Accela Autosampler (see Figure 2), at 30 kg (66 lb) and with a height, width, and depth
of 37 × 38 × 51 cm (14.5 × 15 × 20 in.), is too heavy and too bulky for one person to safely lift
or move alone. In addition, any jarring or uneven movement can cause misalignment of the
autosampler’s XYZ arm.
Figure 2.
Accela Autosampler dimensions
Accela Autosampler
z-axis height
37 cm (14.5 in.)
x-axis width
38 cm (15 in.)
y-axis depth
51 cm (20 in.)
View of the XYZ arm
x-axis
lead screw
XYZ arm
y-axis
stepper
motor
x-axis
stepper
motor
y-axis
lead screw
4
Accela LC System Getting Connected Guide
Thermo Scientific
1 Introduction to the Accela LC System
Lifting and Carrying the Accela System Modules
Accela Open Autosampler
The Accela Open Autosampler with its table (see Figure 3) weighs 38.5 kg (85 lbs) and is too
heavy and bulky for one person alone to lift or move.
Figure 3.
Accela Open Autosampler with table
Height
104.5 cm
(41 in.)
Depth
72 cm
(28 in.)
Width
68 cm
(27 in.)
Thermo Scientific
Accela LC System Getting Connected Guide
5
2
Back Panel Connections
Before you can operate the Accela modules, you must connect them to line power and the
data system computer. In addition, you must connect the contact closure cables to
synchronize the LC pump and the autosampler during an injection sequence and to trigger
the detector when the autosampler releases the injection.
Contents
• System Synchronization Connections
• Communication Connections to the Data System Computer
• Line Power Connections
System Synchronization Connections
The system synchronization (contact closure) connections for an Accela LC system depend on
whether the LC system includes an Accela Autosampler or an Accela Open Autosampler.
To make the system synchronization connections for your LC or LC/MS system, go to the
appropriate topic:
• Accela Autosampler System Synchronization Connections
• Accela Open Autosampler System Synchronization Connections
Thermo Scientific
Accela LC System Getting Connected Guide
7
2
Back Panel Connections
System Synchronization Connections
Accela Autosampler System Synchronization Connections
To make the system synchronization connections for a stand-alone Accela LC system or an
Accela LC system with a Thermo Scientific mass spectrometer, follow these procedures:
• Connecting the System Interconnect Cable to the LC Modules
• Connecting the System Interconnect Cable to the Mass Spectrometer
Note The Accela System Kit contains the system interconnect cable for systems that
include the Accela Autosampler. An Accela system that is ordered with a Thermo
Scientific data system includes this kit. Otherwise, when you order an instrument
separately from a Thermo Scientific data system, you can order the Accela System Kit as
an option.
Connecting the System Interconnect Cable to the LC Modules
If your Accela LC system contains an Accela Autosampler, use the system interconnect cable
shown in Figure 4 to coordinate the timing of the Accela system modules during an injection.
This cable is shipped in the Accela System Kit. See “System Connection Parts and Kits” on
page 63.
Figure 4.
System interconnect cable (P/N 60053-63034)
DETECTOR
To
detector
To
pump
To
pump
PUMP
A/S
To
detector
PUMP
To
autosampler
To
detector
DETECTOR
To
mass spectrometer
DETECTOR
M/S
 To connect the Accela LC modules
1. Plug one of the three connectors labeled DETECTOR into the receptacle located on the
back panel of an Accela detector (see Figure 5).
2. Plug the unlabeled connector with the cable labeled A/S into the receptacle on the back
panel of the autosampler.
3. Plug a connector labeled PUMP into the receptacle on the back panel of the pump.
8
Accela LC System Getting Connected Guide
Thermo Scientific
2 Back Panel Connections
System Synchronization Connections
Figure 5.
System interconnect cable connections for an Accela UHPLC system with an Accela Autosampler
Connector labeled
DETECTOR
Connector labeled
DETECTOR
9
9
7 8
7 8
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
5 6
RS232
4
5 6
0 1
2 3
4
0 1
2 3
UNIT ID
ENET
1 2 3 4 5 6 7 8
–or–
ENET
NC
CHASSIS GND
INTEGRATOR INTEGRATOR +
NC
CHASSIS GND
RECORDER RECORDER +
PURGE
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
GND B
CHB - 1V
CHB - 100mV
CHB - 10mV
GND A
CHA - 1V
CHA - 100mV
CHA - 10mV
EVENT
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
Accela PDA Detector
–or–
Accela UV/Vis Detector
9 10 11 12 13 14 15 16
Accela RI Detector
Cable labeled
A/S
9
9
7 8
7 8
9 10 11 12 13 14 15 16
5 6
1 2 3 4 5 6 7 8
4
5 6
0 1
2 3
4
0 1
2 3
A/S READY
INJECT OUT
INJECT HOLD
PUMP READY
GRAD START
PUMP STOP
+5V @ 150mA
GND
GND
GND
TF 1
TF 2
TF 3
TF 4
+5V @ 150mA
GND
UNIT ID
ENET
Accela Autosampler
System
interconnect
cable
Connector labeled
PUMP
Connector labeled
PUMP
!
RS232
1 2 3 4 5 6 7 8
USB
RS232
DO NOT USE
TOGETHER
WITH
D-SUB25
1 2 3 4 5 6 7 8
USB
RESERVED
GND
INJECT HOLD
PUMP READY
GRAD. START
RESERVED
5V
GND
PRESSURE
GND
INJECT HOLD
PUMP READY
GRAD. START
PUMP STOP
5V
GND
DIGITAL I/O
Accela Pump
Thermo Scientific
–or–
Accela 600 or 1250 Pump
Accela LC System Getting Connected Guide
9
2
Back Panel Connections
System Synchronization Connections
Connecting the System Interconnect Cable to the Mass Spectrometer
To connect a mass spectrometer to an Accela LC system with an Accela Autosampler, follow
the procedure for your mass spectrometer:
• MSQ Plus Mass Detector Connection
• Legacy LCQ Series Connection
• LTQ Series Connection
• TSQ Series Connection
MSQ Plus Mass Detector Connection
The system interconnect cable connector labeled MS detector connects directly to the back
panel of the MSQ Plus Mass Detector.
 To connect an MSQ Plus Mass Detector
Plug the connector with the cable labeled M/S into the User I/O connector (8-pin port)
located on the back panel of the MSQ Plus™ Mass Detector (see Figure 6).
Figure 6.
MSQ Plus Mass Detector trigger connection to an Accela Autosampler
Solvent platform
DETECTOR
Accela detector
Pump
PUMP
Pump
Detector
PUMP
Autosampler
A/S
Detector
DETECTOR
Detector
MS
detector
DETECTOR
M/S
Accela
Autosampler
MSQ Plus
mass spectrometer
USER I/O
Ready Out +
Ready Out -
Accela pump
(Accela Pump,
Accela 600 Pump, or
Accela 1250 Pump)
Not drawn to scale
10
Accela LC System Getting Connected Guide
Interconnect cable
Thermo Scientific
2 Back Panel Connections
System Synchronization Connections
Legacy LCQ Series Connection
To connect a legacy LCQ™ Series mass spectrometer, use the LCQ Series adapter cable
(see Figure 7) and the system interconnect cable.
Figure 7.
LCQ Series adapter cable (P/N 60053-63036)
72 in.
(183 cm)
Plug
connector
Receptacle
connector
 To connect an LCQ Series mass spectrometer
1. Attach the receptacle connector on the LCQ Series interconnect adapter cable to the
system interconnect cable connector with the cable labeled M/S.
2. Connect the plug connector on the adapter cable to the green, 8-pin connection (labeled
Peripheral Controls) located on the upper left side of the LCQ Series mass spectrometer
(see Figure 8).
Figure 8.
LCQ Series mass spectrometer trigger connection to an Accela Autosampler
Solvent platform
DETECTOR
Pump
Interconnect
cable
LCQ Series
mass spectrometer
(back)
Thermo Scientific
Accela
Autosampler
(Left-side
panel)
Interconnect
adapter cable
Not drawn to scale
Accela detector
Pump
PUMP
A/S
Detector
PUMP
Autosampler
Detector
DETECTOR
Detector
MS
detector
DETECTOR
M/S
Accela pump
(Accela Pump,
Accela 600 Pump, or
Accela 1250 Pump)
Peripheral
control
Accela LC System Getting Connected Guide
11
2
Back Panel Connections
System Synchronization Connections
LTQ Series Connection
To connect an LTQ Series (LCQ Fleet™, LXQ™, LTQ XL™ or LTQ Velos™) mass
spectrometer, use the LXQ/LTQ adapter cable (see Figure 9) and the system interconnect
cable.
Figure 9.
LXQ/LTQ adapter cable (P/N 60053-63037)
70 in.
(178 cm)
RED
BLACK
Plug
connector
Receptacle
connector
 To connect an LTQ Series MS detector to the Accela LC system
1. Attach the receptacle connector on the LTQ/LXQ interconnect adapter cable to the
system interconnect cable connector with the cable labeled M/S.
2. Connect the connector end of the LXQ/LTQ interconnect adapter cable to the green,
6-pin connection (labeled Peripheral Control) on the lower right side of the mass
spectrometer (see Figure 10).
Figure 10. LTQ Series mass spectrometer trigger connection to an Accela Autosampler
Solvent platform
DETECTOR
Pump
Accela detector
Pump
PUMP
A/S
Detector
PUMP
Autosampler
Detector
DETECTOR
Detector
MS
detector
DETECTOR
M/S
LCQ Fleet, LXQ, LTQ XL, or LTQ Velos
mass spectrometer
Interconnect
cable
Accela
Autosampler
LC/MS interconnect
adapter cable
Accela pump
(Accela Pump,
Accela 600 Pump, or
Accela 1250 Pump)
Not drawn to scale
12
Accela LC System Getting Connected Guide
Thermo Scientific
2 Back Panel Connections
System Synchronization Connections
TSQ Series Connection
To connect a TSQ™ Series mass spectrometer, use the adapter cable (see Figure 11) and the
system interconnect cable.
Figure 11. TSQ interconnect adapter cable (P/N 60053-63035)
48 in.
(122 cm)
Receptacle
connector
Plug
connector
 To connect a TSQ Series mass spectrometer to the Accela LC system
1. Attach the receptacle connector on the TSQ interconnect adapter cable to the system
interconnect cable connector with the cable labeled M/S.
2. Connect the connector end of the TSQ adapter cable to the receptacle (labeled Start In)
on the lower right side of the TSQ Series mass spectrometer (see Figure 12).
Figure 12. TSQ Quantum Series mass spectrometer trigger connection to an Accela Autosampler
Solvent platform
DETECTOR
Accela detector
Pump
PUMP
A/S
Pump
Detector
PUMP
Autosampler
Detector
DETECTOR
Detector
MS
detector
DETECTOR
M/S
Interconnect cable
Accela
Autosampler
Accela pump
Start In
(Right side
panel)
TSQ Series
mass spectrometer
(back panel)
LC/MS interconnect
adapter cable
Not drawn to scale
Thermo Scientific
Accela LC System Getting Connected Guide
13
2
Back Panel Connections
System Synchronization Connections
Accela Open Autosampler System Synchronization Connections
The system interconnect cables and adapter cables that synchronize the run signals for an LC
or LC/MS system with an Accela Open Autosampler depend on the Accela pump model, the
mass spectrometer model, and whether the LC system includes an Accela detector.
Table 1 lists the system interconnect and adapter cables that Thermo Fisher Scientific supplies
for the Accela Open Autosampler by special order.
Tip An unlabeled cable equivalent to the Accela Open Autosampler interconnect cable
(P/N 60157-63024) ships with the autosampler.
This unlabeled cable has three connectors:
• The DB15 connector plugs into the Interface 1 port on the back panel of the
autosampler.
• The DB25 connector plugs into the D-Sub 25 port on the back panel of an
Accela 600 or 1250 Pump.
• The 2-socket connector connects to the Start In pins of an MSQ or LTQ Series
mass spectrometer.
Table 1. Accela Open Autosampler contact closure and adapter cables
Cable description
Part number
Use
Accela Open
Autosampler system
interconnect cable
60157-63024
Required for all hardware configurations
Accela detector and
MS detector
adapter cable
60157-63026
Required for hardware configurations that
include one or more of the following:
• Accela detector (UV/Vis, PDA, or RI
detector)
• TSQ or Exactive mass spectrometer
Accela Pump
adapter cable
60157-63022
Required for the discontinued Accela Pump
Note Thermo Fisher Scientific has discontinued the Accela Pump. The Accela family of
LC pumps currently includes the Accela 600 Pump and the Accela 1250 Pump.
To make the contact closure connections, follow these procedures as appropriate:
• Connecting the Accela Open Autosampler Interconnect Cable
• Connecting the Adapter Cable for an Accela Detector or TSQ Series MS Detector
• Connecting the Accela Pump Adapter Cable
14
Accela LC System Getting Connected Guide
Thermo Scientific
2 Back Panel Connections
System Synchronization Connections
Connecting the Accela Open Autosampler Interconnect Cable
Figure 13 shows the Accela Open Autosampler interconnect cable (P/N 60157-63024). An
equivalent cable (with unlabeled connectors) ships with the autosampler.
Figure 13. Accela Open Autosampler interconnect cable (P/N 60157-63024)
102 cm (40 in.)
TO PUMP
60157-63024
TO A/S
DB15
connector
DB25
connector
TO MSQ/LTQ/LXQ
2-socket
connector
305 cm (120 in.)
 To connect the Accela Open Autosampler interconnect cable (or equivalent) to the LC
or LC/MS system modules
1. Plug the DB15 connector into the Interface 1 receptacle on the back panel of the Accela
Open Autosampler (see Figure 14).
2. Depending on the Accela pump model, do one of the following:
• If your LC system includes an Accela 600 or 1250 Pump, plug the DB25 connector
into the D-Sub25 receptacle on the back panel of the pump (see Figure 14).
Tip For a dual-pump system, connect the 25-pin connector to the eluting pump.
Do not connect a contact closure cable to the loading pump.
For information about setting up the configuration for the device drivers, refer to
the Accela 600 Pump and Accela 1250 Pump Hardware Manual. For information
about setting up the trigger signal for the loading pump (typically pump 2) and
the pump programs for both pumps, refer to the Accela user guide for your data
system. The dual-pump configuration is not supported by every Thermo
Scientific data system.
–or–
• If your LC system includes an Accela Pump, go to “Connecting the Accela Pump
Adapter Cable” on page 22.
Thermo Scientific
Accela LC System Getting Connected Guide
15
2
Back Panel Connections
System Synchronization Connections
3. Depending on the LC detector or MS detector setup, do one of the following:
• If your LC or LC/MS system includes an Accela detector or a TSQ Series or Exactive
mass spectrometer, go to “Connecting the Adapter Cable for an Accela Detector or
TSQ Series MS Detector” on page 18.
–or–
• If your LC/MS system includes an MSQ Plus Mass Detector or an LTQ Series mass
spectrometer, but does not include an Accela detector, go to step 4.
4. Connect the 2-socket connector to the mass spectrometer as follows:
• For the MSQ Plus Mass Detector, connect the cable to the User I/O Start In pins on
the MS detector’s back panel (see Figure 14).
• For the LTQ Series mass spectrometer, connect the cable to the Peripheral Control
Start In pins on the MS detector’s right panel (see Figure 14).
16
Accela LC System Getting Connected Guide
Thermo Scientific
2 Back Panel Connections
System Synchronization Connections
Figure 14. Accela Open Autosampler interconnect cable connections (back panel view of the LC/MS modules)
Instrument back panels
Accela Open
Autosampler
Accela 600 or
1250 Pump
LTQ Series mass spectrometer
MSQ Plus Mass Detector
TERMINAL
SER1
LAN
MODBUS
BUZZER L
E
D
INTERFACE 1
WASH
STATION
INTERFACE 2
AUX 1
AUX 2
POWER FUSE
USB
USER I/O
AUX
TO A/S
–or–
STACK COOLER DW
START IN +
START IN -
READY OUT +
READY OUT -
60157-63024
RESET
SOURCE
BACKING
DO NOT USE
TOGETHER
WITH
D-SUB25
!
MAINS ON/OFF
355 River Oaks Parkway
San Jose, CA, U.S.A
CAUTION
DISCONNECT INPUT
POWER BEFORE
!
OPENING COVER.
61010 1
Serial No: 925000
REFER ALL SERVICE
TO QUALIFIED
PERSONNEL ONLY.
EN
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
(1) This device may not cause harmful interference,
and (2) This device must accept any interference
received, including interference that may cause
undesired operation.
RS232
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
5,653,876
6,248,157 B1 EP 2107241A2
Futher patent pending
!
EXHAUST
1 2 3 4 5 6 7 8
USB
RESERVED
GND
INJECT HOLD
PUMP READY
GRAD. START
RESERVED
5V
GND
Model: 1250 Pump
D-SUB25
DO NOT COVER
GAS IN
6 BARS MAX
PUMP OUT
LINE
FUSE
100V
115V
T2.0A
230V
T1.0A
50/60 HZ
220VA
MAINS IN
TO PUMP
TO MSQ/LTQ/LXQ
Accela Open
Autosampler
Instrument contact closure pins
INTERFACE 1
MSQ Plus
Mass Detector
–or–
LTQ Series
mass spectrometer
User I/O
Start In +
Start In -
Peripheral Control
Start Ready Start
In
Out Out
TO A/S
TO MSQ/LTQ/LXQ
Ready Out +
Ready Out -
Accela 600 or
1250 Pump
Accela Open Autosampler
interconnect cable
60157-63024
D-SUB25
The equivalent cable that
ships with the autosampler
is unlabeled.
TO PUMP
Thermo Scientific
Accela LC System Getting Connected Guide
17
2
Back Panel Connections
System Synchronization Connections
Connecting the Adapter Cable for an Accela Detector or TSQ Series MS Detector
Use the Accela detector and MS detector adapter cable (see Figure 15) to connect the Accela
Open Autosampler to an Accela detector, a TSQ or Exactive™ mass spectrometer, or both.
Figure 15. Adapter cable for an Accela detector and a TSQ Series MS detector (P/N 60157-63026)
23 cm (9 in.)
TO 60157-63024
8-socket
(female)
connector
60157-63026
TO LCQ FLEET
TO LTQ/LXQ/MSQ
2-pin male
connector
2-socket
(female)
connectors
TO PDA
TO TSQ/EXACTIVE
200 cm (79 in.)
 To connect the LC detector and MS detector adapter cable
1. If you have not already done so, connect the Accela Open Autosampler interconnect cable
to the autosampler and the Accela 600 or 1250 Pump (see “Connecting the Accela Open
Autosampler Interconnect Cable” on page 15).
2. Connect the adapter cable to the interconnect cable by plugging the 2-pin (male)
connector labeled TO 60157-63024 on the adapter cable
into the 2-socket (female) connector on the interconnect cable (see Figure 16).
Tip The Accela Open Autosampler interconnect cable that is available by special
order has three labeled connectors. The 2-socket (female) connector is labeled
TO MSQ/LTQ/LXQ.
An equivalent interconnect cable ships with the Accela Open Autosampler, but its
three connectors are not labeled.
18
Accela LC System Getting Connected Guide
Thermo Scientific
2 Back Panel Connections
System Synchronization Connections
Figure 16. Accela Open Autosampler interconnect cable to detector adapter cable connection
Accela Open
Autosampler
interconnect cable
TO MSQ/LTQ/LXQ
Accela detector and
MS detector
adapter cable
TO 60157-63024
60157-63026
TO LCQ FLEET
60157-63024
TO A/S
TO LTQ/LXQ/MSQ
TO PDA
TO TSQ/EXACTIVE
TO PUMP
3. Using the adapter cable connectors, make the appropriate connections to an Accela
detector, a Thermo Scientific mass spectrometer, or both as follows:
• For an Accela detector (UV/Vis, PDA, or RI), connect the 8-socket connector
attached to the cable labeled TO PDA to pins 1 through 8 on the detector’s back
panel.
• For an MSQ Plus Mass Detector, connect the 2-socket connector attached to the
cable labeled TO LTQ/LXQ/MSQ to the Start In pins on the mass spectrometer’s
back panel.
• For an LTQ Series mass spectrometer, connect the 2-socket connector attached to the
cables labeled TO LTQ/LXQ/MSQ and TO LCQ FLEET to the Start In pins on the
right side of the mass spectrometer.
• For a TSQ or Exactive mass spectrometer, connect the 2-socket connector attached to
the cable labeled TSQ/EXACTIVE to the Start In pins on the right side of the mass
spectrometer.
Figure 17 and Figure 18 show the contact closure connections for a stand-alone Accela LC
system and an LC/MS system, respectively.
Thermo Scientific
Accela LC System Getting Connected Guide
19
2
Back Panel Connections
System Synchronization Connections
Figure 17. Contact closure connections (back panel) for a stand-alone Accela LC system with an Accela Open Autosampler
Interconnect
cable
Accela Open
Autosampler
Accela PDA Detector
–or–
Accela UV/Vis Detector
INTERFACE 1
SER1
LAN
MODBUS
BUZZER L
E
D
INTERFACE 1
WASH
STATION
INTERFACE 2
AUX 1
AUX 2
POWER FUSE
TO A/S
AUX
TERMINAL
8 1
6 7
0 1
0 1
9
9
7 8
230V
TO PDA
OPENING COVER.
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
5, 608, 517
61010 1
50/60 HZ
T1.6A/250V
60157-63024
7 8
115V
T3.15A/250V
5 6
GND B
CHB - 1V
CHB - 100mV
CHB - 10mV
GND A
CHA - 1V
CHA - 100mV
CHA - 10mV
EVENT
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
DISCONNECT INPUT
Accela PDA 80 Hz Detector
100V
ENET
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
REFER ALL SERVICE (1) This device may not cause harmful interference,
and (2) This device must accept any interference
TO QUALIFIED
received, including interference that may cause
PERSONNEL ONLY
undesired operation.
POWER BEFORE
Model:
UNIT ID
CAUTION
355 River Oaks Parkway
San Jose, CA 95134, U.S.A
Serial No:
940XXX
Part No:
60057-60141
Option/Firmware 5cm Lightpipe / 3.00
LINE
FUSE
4
5 6
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
4 5
2 3
4
RS232
2 3
6 7
2 3
4 5
2 3
8 1
STACK COOLER DW
Accela 600 Pump
–or–
Accela 1250 Pump
USE ONLY WITH
250 V FUSES
100VA
DISCONNECT POWER
BEFORE REPLACING FUSES
DO NOT USE
TOGETHER
WITH
D-SUB25
!
355 River Oaks Parkway
San Jose, CA, U.S.A
CAUTION
DISCONNECT INPUT
POWER BEFORE
!
EN
61010 1
Serial No: 925000
REFER ALL SERVICE
TO QUALIFIED
PERSONNEL ONLY.
OPENING COVER.
Model: 1250 Pump
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
(1) This device may not cause harmful interference,
and (2) This device must accept any interference
received, including interference that may cause
undesired operation.
RS232
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
5,653,876
6,248,157 B1 EP 2107241A2
Futher patent pending
!
1 2 3 4 5 6 7 8
USB
RESERVED
GND
INJECT HOLD
PUMP READY
GRAD. START
RESERVED
5V
GND
D-SUB25
D-SUB25
DO NOT COVER
LINE
FUSE
100V
115V
T2.0A
230V
50/60 HZ
T1.0A
220VA
TO PUMP
Adapter cable for the
Accela detector
TO TSQ/EXACTIVE
TO LTQ/LXQ/MSQ
TO LCQ FLEET
60157-63026
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
355 River Oaks Parkway
San Jose, CA 95134 U.S.A.
20
Accela LC System Getting Connected Guide
110V/220V 50/60HZ
FUSE
110V T3.15AL 250V 100VA
220V T1.6AL 250V 100VA
61010 -1
Accela RI Detector
1 2 3 4 5 6 7 8
9 10 11 12 13 14 15 16
NC
CHASSIS GND
INTEGRATOR -INTEGRATOR +
NC
CHASSIS GND
RECORDER -RECORDER +
This device complies with Part 15 of the FCC Rules.
Operation is subject to the following two conditions:
1) This device may not cause harmful interference, and
LINE
TO MSQ/LTQ/LXQ
PURGE
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
NC
CHASSIS GND
INTEGRATOR -INTEGRATOR +
NC
CHASSIS GND
RECORDER -RECORDER +
PURGE
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
ENET
CAUTION
Disconnect Input Power Before Opening Cover
Service by Qualified Personnel
TO 60157-63024
ENET
2) This device must accept any interference received,
including interference that may cause
undesired operation.
Thermo Scientific
2 Back Panel Connections
System Synchronization Connections
Figure 18. Accela Open Autosampler interconnect cable and detector adapter cable connections
MSQ Plus Mass Detector
USER I/O
Start In +
Start In USER I/O
Accela Open
Autosampler
(back panel)
SER1
LAN
MODBUS
BUZZER L
E
D
Ready Out +
Ready Out -
Back panel
INTERFACE 1
WASH
STATION
INTERFACE 2
AUX 1
AUX 2
POWER FUSE
AUX
TERMINAL
START IN +
START IN -
READY OUT +
READY OUT -
TO A/S
LTQ Series mass spectrometer
TO MSQ/LTQ/LXQ
STACK COOLER DW
9
9
5 6
7 8
TO PDA
60157-63024
100V
115V
T3.15A/250V
230V
REFER ALL SERVICE
DISCONNECT INPUT
POWER BEFORE
TO QUALIFIED
OPENING COVER.
PERSONNEL ONLY
ENET
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
(1) This device may not cause harmful interference,
and (2) This device must accept any interference
received, including interference that may cause
undesired operation.
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
5, 608, 517
61010 1
50/60 HZ
T1.6A/250V
Right panel
7 8
GND B
CHB - 1V
CHB - 100mV
CHB - 10mV
GND A
CHA - 1V
CHA - 100mV
CHA - 10mV
Serial No:
940XXX
Part No:
60057-60141
Option/Firmware 5cm Lightpipe / 3.00
LINE
FUSE
UNIT ID
CAUTION
355 River Oaks Parkway
San Jose, CA 95134, U.S.A
Accela PDA 80 Hz Detector
5 6
9 10 11 12 13 14 15 16
EVENT
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
Model:
1 2 3 4 5 6 7 8
4
0 1
2 3
4
0 1
2 3
RS232
USE ONLY WITH
250 V FUSES
100VA
DISCONNECT POWER
BEFORE REPLACING FUSES
DO NOT USE
TOGETHER
WITH
D-SUB25
!
355 River Oaks Parkway
San Jose, CA, U.S.A
CAUTION
DISCONNECT INPUT
POWER BEFORE
!
OPENING COVER.
REFER ALL SERVICE
TO QUALIFIED
PERSONNEL ONLY.
EN
61010 1
Serial No: 925000
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
(1) This device may not cause harmful interference,
and (2) This device must accept any interference
received, including interference that may cause
undesired operation.
RS232
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
5,653,876
6,248,157 B1 EP 2107241A2
Futher patent pending
!
1 2 3 4 5 6 7 8
Peripheral Control
USB
RESERVED
GND
INJECT HOLD
PUMP READY
GRAD. START
RESERVED
5V
GND
Model: 1250 Pump
Start Ready Start
In
Out Out
D-SUB25
DO NOT COVER
LINE
FUSE
100V
115V
T2.0A
230V
T1.0A
50/60 HZ
220VA
Peripheral Control
Start Ready Start
In
Out Out
TO PUMP
Interconnect
cable
TSQ Series mass spectrometer
Right panel
Adapter
cable
+ 30V Max
TO TSQ/EXACTIVE
+ 30V Max
Start In
60157-63026
Thermo Scientific
TO LTQ/LXQ/MSQ
TO 60157-63024
TO LCQ FLEET
TO MSQ/LTQ/LXQ
Start In
Accela LC System Getting Connected Guide
21
2
Back Panel Connections
System Synchronization Connections
Connecting the Accela Pump Adapter Cable
Use the Accela Open Autosampler interconnect cable (see Figure 13 on page 15) and the
Accela Pump adapter cable (see Figure 19) to interconnect the system modules for these
hardware configurations:
• Accela Open Autosampler, Accela Pump, and MSQ Plus Mass Detector
• Accela Open Autosampler, Accela Pump, and an LTQ Series (LCQ Fleet, LXQ, LTQ XL,
or LTQ Velos) mass spectrometer
Figure 19. Accela Pump adapter cable
23 cm (9 in.)
TO 60157-63024
60157-63022
TO PUMP
 To connect the Accela Pump adapter cable
1. If you have not already done so, plug the DB15 connector of the Accela Open
Autosampler interconnect cable into the Interface 1 port of the Accela Open
Autosampler.
2. Connect the 25-pin connector of the Accela Pump adapter cable to the 25-pin connector
of the Accela Open Autosampler interconnect cable (see Figure 20).
TO MSQ/LTQ/LXQ
TO
TO
TO
TO
TO
TO
TO
TO
A/S
A/S
A/S
A/S
A/S
A/S
A/S
A/S
TO PUMP
Figure 20. Accela Open Autosampler Interconnect cable to Accela Pump adapter cable
connection
60157-63024
TO PUMP
Accela Open Autosampler
interconnect cable
TO 60157-63024
60157-63022
Accela Pump
adapter cable
3. Connect the 8-pin connector of the Accela Pump adapter cable to pins 1 through 8 on
the back panel of the Accela Pump (see Figure 21).
4. Connect the 2-pin connector of the Accela Open Autosampler interconnect cable to the
mass spectrometer (see “Connecting the Accela Open Autosampler Interconnect Cable”
on page 15).
22
Accela LC System Getting Connected Guide
Thermo Scientific
2 Back Panel Connections
System Synchronization Connections
Figure 21. Accela Open Autosampler interconnect cable and Accela Pump adapter cable connections
INTERFACE 1
Peripheral Control
Accela Open
Autosampler
(back panel)
Start Ready Start
In
Out Out
SER1
LAN
MODBUS
BUZZER L
E
D
INTERFACE 1
WASH
STATION
INTERFACE 2
AUX 1
AUX 2
POWER FUSE
AUX
TO A/S
TERMINAL
60157-63024
STACK COOLER DW
Accela Pump
(back panel)
!
CAUTION
POWER BEFORE
OPENING COVER.
!
POWER BEFORE
TO QUALIFIED
OPENING COVER.
PERSONNEL ONLY.
Part No:
60057-60010
and (2) This device must accept any interference
TO QUALIFIED
received,
including that
interference
that may cause
received,
interference
may cause
PERSONNEL
ONLY. including
undesired operation.
undesired operation.
RS232
This product is This
manufactured
under, and under,
covered
product is manufactured
and covered by,
by, one or moreone
of or
themore
following
U.S. patents:
of the following
U.S. patents:
EN
61010 1
61010 - 1
5,653,876
5,653,876
6,248,157
B1 EP 2107241A2
6,248,157B1
Futher patent pending
!
1 2 3 4 5 6 7 8
USB
RESERVED
GND
INJECT HOLD
PUMP READY
GRAD. START
RESERVED
5V
GND
Serial No:
Options/Firmware: 12472
DO NOT USE
TOGETHER
WITH
D-SUB25
This device complies
withcomplies
FCC Rules,
PartRules,
15. Part 15.
This device
with FCC
CAUTION
River Oaks Parkway
Operation is subject
to the
following
conditions
355 River Oaks355
Parkway
Operation
is subject
to the
following two conditions:
REFER ALL SERVICE REFER ALL
DISCONNECT
INPUT
Jose, CA 95134
U.S.A.
(1) SERVICE
This device (1)
may
cause
interference,
Thisnot
device
mayharmful
not cause
harmful interference,
DISCONNECT INPUT
San Jose, CA, San
U.S.A
and (2) This device must accept any interference
Model: Accela Pump
Model: 1250 Pump
Serial No: 925000
D-SUB25
DO NOT COVER
LINE
FUSE
100V
115V
T2.0A
230V
T1.0A
50/60 HZ
220VA
TO MSQ/LTQ/LXQ
LTQ Series
mass spectrometer
(right panel)
Interconnect
cable
MSQ Plus
Mass Detector
(back panel)
USER I/O
USB
USER I/O
Adapter
cable
Start In +
Start In -
Start In +
Start In -
Ready Out +
Ready Out -
RESET
SOURCE
Ready Out +
Ready Out -
BACKING
MAINS ON/OFF
EXHAUST
GAS IN
6 BARS MAX
PUMP OUT
MAINS IN
Thermo Scientific
Accela LC System Getting Connected Guide
23
2
Back Panel Connections
Communication Connections to the Data System Computer
Communication Connections to the Data System Computer
To connect the Accela modules to the data system computer, follow these procedures:
• Connecting the Accela Autosampler and Detector to the Data System Computer
• Connecting the Pump to the Data System Computer
• Connecting the Accela Open Autosampler to the Data System Computer
Connecting the Accela Autosampler and Detector to the Data System Computer
The Accela Autosampler and Accela detector (UV/Vis, PDA, or RI) communicate with the
data system computer through an Ethernet connection (see Figure 22) routed through an
Ethernet switch connected to the data system computer.
To make the Ethernet connections, use the RJ-45, CAT-5 shielded Ethernet cables provided
in the instrument accessory kits. These cables are 2 m (7 ft) long.
When you order the Accela LC system with a Thermo Scientific mass spectrometer, the
Ethernet card for the LC/MS system is preset to the appropriate IP address (172.16.0.101).
While installing the LC or LC/MS system, your local Thermo Fisher Scientific checks the IP
address setting.
 To connect the Ethernet cables
1. To connect an Accela Autosampler or Accela detector (UV/Vis, RI, or PDA) to the
Ethernet switch (see Figure 22), do the following:
a. Plug an Ethernet cable into the Ethernet port on the back panel of the module.
b. Plug the other end of the cable into the Ethernet switch.
24
Accela LC System Getting Connected Guide
Thermo Scientific
2 Back Panel Connections
Communication Connections to the Data System Computer
Figure 22. Ethernet connections
Solvent
platform
Accela
detector
Computer
ENET
Accela
Autosampler
Ethernet
switch
Accela
pump
RJ-45, CAT-5 shielded
Ethernet cables
2. To connect the Ethernet switch to the data system computer (see Figure 22), do the
following:
a. If necessary, verify that the IP address for the dedicated Ethernet card is set to
172.16.0.101.
b. Plug one end of an RJ-45, CAT-5, 2 m (7 ft) Ethernet cable into the appropriate
Ethernet port in the data system computer.
c. Plug the other end of the cable into the Ethernet switch.
 To troubleshoot the Ethernet connection for an Accela device
1. From the Windows XP desktop, choose Start > Run.
The Run dialog box appears (see Figure 23).
Thermo Scientific
Accela LC System Getting Connected Guide
25
2
Back Panel Connections
Communication Connections to the Data System Computer
Figure 23. Run dialog box
2. In the Open list, type or select CMD.
The Command Prompt window appears.
3. At the command prompt, type ping, press SPACEBAR, and type the IP address for the
Accela device:
172.16. x .y
Where:
x = the stack setting with a range from 1 to 99
y = a defined value
Device
IP address
Accela PDA Detector
172. 16. x. 162
Accela UV/Vis Detector
172. 16. x. 171
Accela Autosampler
172. 16. x. 111
4. Press ENTER.
When you type the correct IP address, the ping statistics for the IP address appear.
26
Accela LC System Getting Connected Guide
Thermo Scientific
2 Back Panel Connections
Communication Connections to the Data System Computer
Connecting the Pump to the Data System Computer
The Accela pump (Accela Pump, Accela 600 Pump, or Accela 1250 Pump) communicates
with the data system computer through a USB cable.
 To connect the USB cable to the Accela pump
1. Locate the USB cable, consisting of a series A plug on one end and a series B plug on the
other end (see Figure 24).
Figure 24. USB cable connection plugs
Series A socket
Series B socket
Series A plug
Series B plug
2. Connect the series B plug to the port marked USB on the back of the Accela pump.
3. Connect the series A plug to a USB slot on the data system computer (see Figure 25).
Figure 25. USB connection between an Accela pump and the data system computer
Computer
USB
Accela
pump
USB cable
Thermo Scientific
Accela LC System Getting Connected Guide
27
2
Back Panel Connections
Communication Connections to the Data System Computer
Connecting the Accela Open Autosampler to the Data System Computer
The Accela Open Autosampler communicates with the data system computer through a serial
connection (see Figure 26).
 To connect the serial communication cable
1. Connect one end of the DB9 to DB9 serial communication cable to the SER 1 port on
the back panel of the Accela Open Autosampler.
2. Connect the other end of the cable to the serial port on the back panel of the data system
computer.
Figure 26. Accela Open Autosampler connection to the data system computer
SER1 port
SER1
LAN
MODBUS
BUZZER L
E
D
WASH
STATION
INTERFACE 1
INTERFACE 2
AUX 1
AUX 2
POWER FUSE
AUX
TERMINAL
DB9 to DB9 serial
communication cable
STACK COOLER DW
PELTIER
THERMOSTAT
Input: 100V – 240V
2A
50/60 HZ
Output: 24V
4.5A
I
O
ETL LISTED
CONFORMS TO
UL STD 61010A-1
CERTIFIED TO
CAN/USA C22.2 STD NO. 61010-1
36V
PAL
28
Accela LC System Getting Connected Guide
4.16A
AUX
Thermo Scientific
2
Back Panel Connections
Line Power Connections
Line Power Connections
For information about the power entry module and fuses for the Accela modules, refer to the
hardware manual for each module.
To connect your Accela system to line power, follow these procedures:
• Connecting the Accela Autosampler, Detector, and Pump to Line Power
• Connecting the Accela Open Autosampler to Line Power
Connecting the Accela Autosampler, Detector, and Pump to Line Power
With the exception of the Accela Open Autosampler, the Accela modules require one line
power outlet each.
Although these Accela modules ship with power cords appropriate to their shipping
destination (see Table 2), local codes in your area might require you to install another type of
plug and receptacle. The Thermo Fisher Scientific field service engineer for your country will
provide the appropriate power plugs.
Table 2. Power cords supplied according to shipping destination
Destination
Plug type
Voltage rating
Current rating
Part number
United Kingdom
BS 1363
250 Vac
5A
6003-0810
Switzerland
SEV 1011
250 Vac
10 A
6003-0620
Europe
CEE 7/7
250 Vac
10 A
6003-0330
United States
and Canada
NEMA 5-15P
125 Vac
10 A
6003-0160
China
C13 connector
250 Vac
10 A
00302-99-00026
 To connect the Accela Autosampler, Accela detector, and Accela pump to line power
1. For the discontinued Accela PDA (20 Hz) Detector and the Accela UV/Vis Detector,
check the voltage selector setting by looking through the cut-out window on the power
entry module.
For information about checking the voltage selector, refer to the hardware manuals for
those products.
Note The Accela PDA (80 Hz) Detector has an auto-sensing power entry module
that uses 250 V fuses.
2. Ensure that the power switch at the front of the module is in the Off position (that is,
released or out).
3. Plug the power cord into the power receptacle on the back panel of the module and
connect the other end to line power (see Figure 27).
Thermo Scientific
Accela LC System Getting Connected Guide
29
2
Back Panel Connections
Line Power Connections
Figure 27. Accela Autosampler, Accela pump, and Accela detector line power connections
Solvent
platform
Accela PDA or UV/Vis
Detector
or
Accela RI
Detector
Accela
Autosampler
Accela
pump
Line power
Power cords
30
Accela LC System Getting Connected Guide
Thermo Scientific
2
Back Panel Connections
Line Power Connections
Connecting the Accela Open Autosampler to Line Power
The Accela Open Autosampler requires two line power outlets: one for the power supply
module and one for the stack cooler.
 To connect an Accela Open Autosampler to line power
1. To connect the power supply module (see Figure 28), do the following:
a. Turn the power supply module’s power switch to the Off position.
b. Connect one end of the DC power cable to the Power port on the back panel of the
Accela Open Autosampler and the other end to the PAL port on the back of the
power supply module.
c. Plug the female end of the power cord into the receptacle on the front of the power
supply module and the male end of the power cord into the line power outlet.
Figure 28. Accela Open Autosampler power supply module connections
Power port on the back panel
of the autosampler
POWER FUSE
ETL LISTED
CONFORMS TO
UL STD 61010A-1
CERTIFIED TO
CAN/USA C22.2 STD NO. 61010-1
36V
PAL
4.16A
100V – 240 V ~ 4A 50/60 HZ
!
I
0
AUX 2
AUX
Power supply module
On/Off switch
PAL port on the back of the
power supply module
2. To connect the stack cooler (see Figure 29), do the following:
a. Turn the temperature control unit’s power switch to the Off position.
Thermo Scientific
Accela LC System Getting Connected Guide
31
2
Back Panel Connections
Line Power Connections
b. Connect the cable that extends from the back of the stack cooler to the Peltier
Thermostat port on the back of the temperature control unit.
c. Plug the female end of the power cord into the receptacle on the back of the
temperature control unit and the male end of the power cord into the line power
outlet (see Figure 29).
Figure 29. Accela Open Autosampler stack cooler connections
STACK COOLER DW
Stack cooler
(back panel)
Thermostat control unit
(back panel)
PELTIER
THERMOSTAT
Input: 100V – 240V
2A
50/60 HZ
Output: 24V
4.5A
Power
switch
I
O
Line power
connection
Figure 30 shows the line power connections for an Accela LC system with an Accela Open
Autosampler.
32
Accela LC System Getting Connected Guide
Thermo Scientific
2
Back Panel Connections
Line Power Connections
Figure 30. Accela Open Autosampler line power connections
Cable connecting
the autosampler to
the power supply unit
SER1
LAN
MODBUS
BUZZER L
E
D
INTERFACE 1
WASH
STATION
INTERFACE 2
AUX 1
AUX 2
POWER FUSE
AUX
TERMINAL
STACK COOLER DW
9
9
7 8
LINE
100V
115V
T3.15A/250V
230V
ENET
GND B
CHB - 1V
CHB - 100mV
CHB - 10mV
GND A
CHA - 1V
CHA - 100mV
CHA - 10mV
EVENT
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
DISCONNECT INPUT
REFER ALL SERVICE
POWER BEFORE
Accela PDA 80 Hz Detector
TO QUALIFIED
OPENING COVER.
Serial No:
940XXX
Part No:
60057-60141
Option/Firmware 5cm Lightpipe / 3.00
FUSE
UNIT ID
CAUTION
355 River Oaks Parkway
San Jose, CA 95134, U.S.A
Model:
7 8
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
5 6
5 6
0 1
2 3
4
0 1
2 3
RS232
4
Cable connecting the
thermostat control unit
to the stack cooler
PERSONNEL ONLY
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
(1) This device may not cause harmful interference,
and (2) This device must accept any interference
received, including interference that may cause
undesired operation.
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
5, 608, 517
61010 1
50/60 HZ
T1.6A/250V
USE ONLY WITH
250 V FUSES
100VA
DISCONNECT POWER
BEFORE REPLACING FUSES
!
Thermostat control unit
355 River Oaks Parkway
San Jose, CA, U.S.A
Input: 100V – 240V
2A
50/60 HZ
Output: 24V
4.5A
CAUTION
DISCONNECT INPUT
POWER BEFORE
!
OPENING COVER.
Model: 1250 Pump
PERSONNEL ONLY.
EN
61010 1
Serial No: 925000
I
Power supply unit
REFER ALL SERVICE
TO QUALIFIED
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
(1) This device may not cause harmful interference,
and (2) This device must accept any interference
received, including interference that may cause
undesired operation.
RS232
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
5,653,876
6,248,157 B1 EP 2107241A2
Futher patent pending
!
DO NOT USE
TOGETHER
WITH
D-SUB25
1 2 3 4 5 6 7 8
USB
RESERVED
GND
INJECT HOLD
PUMP READY
GRAD. START
RESERVED
5V
GND
PELTIER
THERMOSTAT
D-SUB25
DO NOT COVER
O
LINE
FUSE
100V
115V
T2.0A
230V
T1.0A
50/60 HZ
220VA
ETL LISTED
CONFORMS TO
UL STD 61010A-1
CERTIFIED TO
CAN/USA C22.2 STD NO. 61010-1
36V
PAL
4.16A
AUX
Power cord from the
thermostat control unit
Power cord from the
power supply unit
Thermo Scientific
Accela LC System Getting Connected Guide
33
3
Solvent Line Connections
To connect the low- and high-pressure solvent lines for the Accela UHPLC system, follow the
procedures in this chapter.
Contents
• Routing the Tubing
• Connecting the Wash Bottle Tubing to the Syringe Valve
• Connecting the Needle Tubing to the Syringe Valve
• Low-Pressure Solvent Reservoir Line Connections
• High-Pressure Connection between the Pump and the Autosampler
• LC Column Installation
• Connecting the RI Detector Outlet to Waste
Routing the Tubing
The enclosures and drip trays for the Accela modules are designed to hold the low-pressure
and high-pressure solvent lines out of view behind the module doors.
 To secure the solvent lines behind the module doors
Do the following:
• Route the low-pressure solvent lines through the cutouts on the top-left side of the
enclosures and the left side of the drip trays.
• Route the high-pressure solvent lines through the access slots on the top-right side of
the enclosures and the right side of the drip trays.
Figure 31 shows the cutouts in the Accela PDA Detector enclosure and drip tray.
Thermo Scientific
Accela LC System Getting Connected Guide
35
3
Solvent Line Connections
Routing the Tubing
Figure 31. Cutouts in the enclosure and drip tray
Right door
Left door
Access slot in the top
right of the enclosure
Cutout in the top left
of the enclosure
Cutout in the left side
of the drip tray
Access slot in the right side
of the drip tray
36
Accela LC System Getting Connected Guide
Thermo Scientific
3 Solvent Line Connections
Connecting the Wash Bottle Tubing to the Syringe Valve
Connecting the Wash Bottle Tubing to the Syringe Valve
The Wash Bottle Kit contains a 1 L bottle and the wash bottle tubing assembly. A Teflon™
solvent filter is attached to one end of the tubing, and a nut and ferrule fitting is attached to
the other end of the tubing (see Figure 32).
Figure 32. Wash bottle tubing assembly
Cap assembly
Filter
Fitting
 To connect the wash bottle tubing to the syringe valve
1. Fill the wash bottle with the appropriate solvent.
Note The autosampler uses the solvent in the wash bottle as the transfer solvent for
injections. Unless you specify otherwise, the autosampler also uses this solvent as the
flush solvent at the end of an injection cycle.
2. Place the end of the tubing with the Teflon solvent filter into the bottom of the bottle.
3. Snap the two cap sections together, and then screw the bottle cap onto the wash bottle.
4. Screw the fitting into the left side of the syringe valve (see Figure 33).
Thermo Scientific
Accela LC System Getting Connected Guide
37
3
Solvent Line Connections
Connecting the Needle Tubing to the Syringe Valve
Figure 33. Wash bottle tubing connection to the syringe valve
Wash tubing
fitting
Syringe
valve
Needle tubing
fitting
Connecting the Needle Tubing to the Syringe Valve
The Accela Autosampler ships with the needle tubing attached to the needle assembly. The
tubing is routed through a bracket on the XYZ arm, a bracket on the left side of the tray
compartment wall, and a bracket on the tray compartment door. In addition, the
two-pronged needle guide is connected to the x-axis positioning frame, and the PVC cap is
pushed below the metal runner for the x-axis positioning frame.
 To complete the installation of the needle tubing
Connect the externally-threaded fitting to the right side of the syringe valve
(see Figure 33).
Low-Pressure Solvent Reservoir Line Connections
Four low-pressure solvent lines connect the solvent bottles held by the solvent platform to the
inlet ports of the pump’s built-in vacuum degasser.
To connect the solvent reservoir lines to the pump, follow the instructions in the section for
your pump:
• Connecting the Solvent Reservoir Lines to the Accela Pump
• Connecting the Solvent Reservoir Lines to the Accela 600 or Accela 1250 Pump
38
Accela LC System Getting Connected Guide
Thermo Scientific
3 Solvent Line Connections
Low-Pressure Solvent Reservoir Line Connections
Connecting the Solvent Reservoir Lines to the Accela Pump
To assemble the solvent bottles and then attach the solvent lines to the inlet ports of the
built-in vacuum degasser of the Accela pump, follow these procedures:
• Assembling the Solvent Bottles for the Accela Pump
• Connecting the Solvent Lines to the Accela Pump Degasser
Assembling the Solvent Bottles for the Accela Pump
 To assemble the solvent reservoir bottles
1. Cut the 6 m (20 ft) length of FEP tubing provided in the Solvent Interconnect Kit into
four 1.5 m (5 ft) lengths.
2. Label one of the four solvent reservoir bottle caps “A.”
3. Pass the tubing through the solvent reservoir bottle cap labeled A.
4. Connect the 10 μm Teflon™ filter to the end of the solvent line (see Figure 34).
Figure 34. Solvent reservoir cap assembly with the solvent line assembly
Solvent reservoir
bottle cap
1/4 × 28 Super Flangeless
fittings
Solvent reservoir
filter
(10 μm particle size,
Teflon)
5. Insert the solvent reservoir filter and inlet line into one of the solvent reservoir bottles,
and screw the cap onto the solvent reservoir bottle until it is secure.
Thermo Scientific
Accela LC System Getting Connected Guide
39
3
Solvent Line Connections
Low-Pressure Solvent Reservoir Line Connections
Tip The cap is a two-piece assembly. The upper section snaps onto a threaded section.
You can screw the threaded section onto the bottle and snap on the upper section after
installing the tube, or, if you are replacing existing tubing, you can unscrew the entire
cap from the bottle.
The ID of the upper section is 44 mm (1.75 in.). If necessary, you can use this section
alone for wide-mouth solvent bottles.
6. Position the bottle in the solvent bottle holder, allowing the solvent inlet line to hang
down along the left side of the system.
7. Repeat steps 1 through 5 for solvents B, C, and D if applicable.
Connecting the Solvent Lines to the Accela Pump Degasser
Use Super Flangeless™ fittings to connect the solvent lines to the inlet ports of the Accela
pump’s built-in vacuum degasser. The Super Flangeless fitting consists of three components: a
nut, a ferrule, and a stainless steel compression ring (see Figure 35). The compression ring has
two sides: a flat side and an angled side (see Figure 36). The flat side of the ring faces the nut,
and the angled side of the ring faces the narrow end of the ferrule.
Figure 35. Super Flangeless fitting components
Nut
Compression ring
Ferrule
Figure 36. Flat and angled sides of the steel compression ring
Angled side
Flat side
40
Accela LC System Getting Connected Guide
Thermo Scientific
3 Solvent Line Connections
Low-Pressure Solvent Reservoir Line Connections
 To connect the solvent lines to the degasser inlets of the Accela Pump
1. Slide the three components of the Super Flangeless fitting onto the remaining end of the
solvent line (see Figure 35):
a. Slide the nut onto the end of the tubing.
b. With the flat side of the compression ring facing the nut, slide the compression ring
onto the end of the tubing.
c. With the narrow end of the ferrule facing the compression ring, slide the ferrule onto
the end of the tubing.
2. Swage the Super Flangeless fitting onto the tubing:
a. Create a compression tool by screwing a Super Flangeless nut into one end of a
1/4 in. × 28 thread internal union (see Figure 37).
Figure 37. Compression tool created by screwing a Super Flangeless nut into a union
Union
Nut
Compression tool
b. Insert the tubing with the fitting assembly into the other end of the union.
c. Hold the tubing to the bottom of the tool while tightening the nut.
d. Unscrew the swaged fitting from the compression tool and verify the following:
• The end of the square-cut tubing is flush with the end of the ferrule
(see Figure 38).
• The steel compression ring is seated over the ferrule (see Figure 38).
Figure 38. Super Flangeless fitting swaged onto the tubing
3. Insert the tubing with the properly swaged fitting into inlet port A (see Figure 39), and
then tighten the nut fingertight. Be careful not to cross-thread the fitting, which can
cause solvent leakage.
Thermo Scientific
Accela LC System Getting Connected Guide
41
3
Solvent Line Connections
Low-Pressure Solvent Reservoir Line Connections
Figure 39. Vacuum degasser connections
Vacuum degasser
inlets and outlets
4. To complete the installation, route the solvent line through the cutouts along the left side
of each Accela module.
5. Repeat the above steps for the B, C, and D solvent inlet lines, if applicable.
Connecting the Solvent Reservoir Lines to the Accela 600 or Accela 1250 Pump
The Accela 600 or Accela 1250 Pump Inlet Tubing Kit (see “Accela 600 and Accela 1250
Pump Inlet Tubing Kit” on page 65) contains preassembled, low-pressure solvent lines that
have a stainless steel filter at one end and a flangeless fitting at the other end. The Solvent
Interconnect Kit (see “Solvent Interconnect Kit” on page 65) contains the solvent reservoir
bottles, the solvent bottle caps and adapters, the solvent bottle labels, and the solvent line
clip-on markers.
To install the solvent reservoir lines for the Accela 600 or Accela 1250 pump, follow these
procedures:
• Assembling the Solvent Bottles for the Accela 600 or Accela 1250 Pump
• Connecting the Solvent Bottles to the Accela 600 or Accela 1250 Pump Degasser
Assembling the Solvent Bottles for the Accela 600 or Accela 1250 Pump
 To assemble the solvent reservoir bottles
1. Label one of the four solvent reservoir bottle caps “A.”
2. Pull the filter off the end of the solvent line, and then pass the tubing through the solvent
reservoir bottle cap labeled A.
3. Reconnect the filter to the end of the solvent line (see Figure 40).
IMPORTANT Use only stainless steel, 20 μm particle size sinker frits to terminate the
solvent reservoir lines, as provided in the accessory kit.
42
Accela LC System Getting Connected Guide
Thermo Scientific
3 Solvent Line Connections
Low-Pressure Solvent Reservoir Line Connections
Figure 40. Solvent reservoir cap assembly with the solvent line assembly
Solvent reservoir
bottle cap
Solvent reservoir
filter
(20 μm particle size,
stainless steel)
1/4 in. × 28 PEEK fitting
and Tefzel ferrule
Note Each solvent line has a different color fitting. The colors are yellow, green,
orange, and blue. Matching the color of the inlet line fitting to the outlet line fitting is
important for ensuring that the correct solvent goes into and out of the degasser lines
and into the inlet ports of the pump proportioning valve.
4. Insert the solvent reservoir filter and inlet line into one of the solvent reservoir bottles,
and screw the cap onto the solvent reservoir bottle until it is secure.
Tip The cap is a two-piece assembly. The upper section (see Figure 40) snaps onto a
threaded section. You can screw the threaded section onto the bottle and snap on the
upper section after installing the tube, or, if you are replacing existing tubing, you can
unscrew the entire cap from the bottle.
5. Position the bottle in the solvent bottle holder, allowing the solvent inlet line to hang
down along the left side of the system.
6. Repeat steps 1 through 5 for solvents B, C, and D, if applicable.
Thermo Scientific
Accela LC System Getting Connected Guide
43
3
Solvent Line Connections
Low-Pressure Solvent Reservoir Line Connections
Connecting the Solvent Bottles to the Accela 600 or Accela 1250 Pump Degasser
Use HPLC-grade solvents that are free of particulate matter and terminate the solvent
reservoir lines with the stainless steel, 20 μm particle size sinker frits provided in the
Accela 600 Pump Inlet Tubing Kit.
CAUTION Do not use solvents containing Freon™ or perfluorinated solvents, such as
Fluorinert™, FC-75, FC-40, and Fomblin™ perfluoro polyether solvents from Solvay
Solexis, Inc. They adversely affect the Teflon AF degassing membrane.
CAUTION To prevent personal injury, observe good laboratory practice when handling
solvents or changing tube lines. Consult the pertinent material safety data sheets for the
solvents used for HPLC analysis.
 To connect the solvent lines to the degasser inlets of an Accela 600 or Accela 1250
Pump
Connect the solvent lines with their colored flangeless fittings (see Figure 41) to the inlet
ports of the pump’s built-in degassing unit (see Figure 41), matching the color of the
fitting to the color of the adjacent outlet port (see Figure 42).
Figure 41. Low-pressure solvent line with flangeless fitting
Figure 42. Vacuum degasser connections
Vacuum degasser
inlets and outlets
44
Accela LC System Getting Connected Guide
Thermo Scientific
3 Solvent Line Connections
High-Pressure Connection between the Pump and the Autosampler
High-Pressure Connection between the Pump and the Autosampler
When you are operating the LC pump at pressures above 276 bar (4000 psi), you must use
stainless steel tubing and fittings to connect the solvent path from the pump outlet to the
autosampler inlet.
IMPORTANT Avoid using PEEK™ tubing and fittings with system pressures above 276 bar
(4000 psi). Thermo Fisher Scientific ships precut 1/16 in. OD stainless steel tubing and
two-piece compression fittings with the Accela Autosampler and Accela Open
Autosampler.
The high-pressure solvent lines between the pump’s liquid displacement assembly and its
outlet are installed at the factory:
• For the Accela Pump, these solvent lines connect the liquid displacement assembly (LDA)
outlet to the inline filter and the inline filter to the dynamic mixer.
Note The inline filter captures particulate matter before it reaches the autosampler
heat exchanger.
• For the Accela 600 or Accela 1250 Pump, these solvent lines connect the LDA to the
static mixer and the static mixer to the placeholder module.
Note The static mixer provided with the Accela 600 and Accela 1250 Pumps contains
a 10 μm particle size filter, so an additional inline filter is not necessary.
CAUTION To prevent personal injury caused by skin contact with hazardous solvents,
turn off the pump flow before you connect the high-pressure solvent line between the LC
pump and the autosampler.
To connect the high-pressure solvent line between the pump and the autosampler, follow
these procedures:
• Connecting the High-Pressure Solvent Line to the Pump Outlet
• Connecting the High-Pressure Solvent Line to the Autosampler
Connecting the High-Pressure Solvent Line to the Pump Outlet
When you operate the Accela pump at high pressures, use high-pressure, stainless steel tubing
and fittings.
Tip To identify the tubing ID, the precut tubing has a color-coded band:
Thermo Scientific
• Red = 0.005 in.
• Black = 0.007 in.
• Blue = 0.010 in.
• Yellow = 0.020 in.
Accela LC System Getting Connected Guide
45
3
Solvent Line Connections
High-Pressure Connection between the Pump and the Autosampler
Figure 43 shows the two-piece compression fitting supplied with the Accela Accessory Kit.
The lengths of the nut and ferrule are 0.45 inches and 0.188 inches, respectively.
Tip The Accela autosamplers have UHPLC Valco injection valves. You can use the
1/16 in. fittings for the Valco Cheminert UHPLC injection valve to connect the pump
outlet to the autosampler inlet. The lengths of the nut and ferrule are 0.43 in. and 0.188
in., respectively.
Figure 43. Two-piece compression fitting
0.45 in.
0.188 in.
 To connect the high-pressure solvent line to the LC pump outlet
1. Using the supplied two-piece compression fitting (see Figure 43), connect a length of
0.005 in. ID × 1/16 in. OD tubing to the pump outlet.
The tubing length depends on the pump and autosampler models:
• To connect an Accela pump to the Accela Autosampler, use these precut tubing
lengths:
–
For the Accela Pump, connect a 20 cm (8 in.) length of precut tubing to the
outlet of the dynamic mixer.
–
For the Accela 600 Pump or Accela 1250 Pump, connect a 26 cm (10 in.) length
of precut tubing to the outlet of the placeholder module or the optional dynamic
mixer.
• To connect an Accela pump (Accela Pump, Accela 600 Pump, or Accela 1250 Pump)
to the Accela Open Autosampler, use the 0.6 m (2 ft) length of precut tubing
supplied with the Accela Open Autosampler.
2. Using a 1/4 in. open-ended wrench, tighten the nut.
46
Accela LC System Getting Connected Guide
Thermo Scientific
3 Solvent Line Connections
High-Pressure Connection between the Pump and the Autosampler
Connecting the High-Pressure Solvent Line to the Autosampler
To connect the high-pressure solvent line to the autosampler, go to the appropriate procedure:
• Connecting the Solvent Line to the Accela Autosampler Heat Exchanger
• Connecting the Solvent Line to the Accela Open Autosampler Injection Valve
Connecting the Solvent Line to the Accela Autosampler Heat Exchanger
Before you connect the solvent line to the Accela Autosampler’s heat exchanger inlet, check
the column oven temperature. The metal plate in front of the Accela Autosampler heat
exchanger can reach temperatures as high as 95 °C (203 °F).
CAUTION To prevent personal injury, before installing the LC column or connecting the
pump to the autosampler, ensure that the column oven compartment is at room
temperature. The column oven compartment can reach temperatures as high as 95 °C
(203 °F).
Figure 44 shows the Hot Surface caution symbols on the column oven surface.
Figure 44. Hot surface cautions on the surface of the Accela Autosampler column oven
Hot surface caution
symbols
 To connect the solvent line to the heat exchanger inlet
1. Ensure that the column oven compartment is at room temperature and that the pump
flow is turned off.
2. Route the high-pressure tubing through the access slot in the autosampler drip tray.
3. Using a two-piece compression fitting (see Figure 43 on page 46), connect the tubing to
the heat exchanger inlet.
4. Using a 1/4 in. open-ended wrench, tighten the nut.
Figure 45 and Figure 46 show the high-pressure connection between the autosampler and the
Accela Pump or the Accela 600 Pump or Accela 1250 Pump, respectively.
Thermo Scientific
Accela LC System Getting Connected Guide
47
3
Solvent Line Connections
High-Pressure Connection between the Pump and the Autosampler
Figure 45. Connection between the Accela Pump and the autosampler
20
µL
3
4
2
5
1
6
Connection to the
heat exchanger inlet
Access slot in the
autosampler drip tray
Dynamic mixer
PU RGE
Inline filter holder
PULSE
DAMPENER
FLUSH
Figure 46. Connection between the Accela 600 or Accela 1250 Pump and the Accela Autosampler
20
µL
3
4
2
5
1
6
Connection to the
heat exchanger
inlet
Access slot in the
autosampler
drip tray
Dynamic
mixer
–or–
Placeholder
module
Static mixer
48
Accela LC System Getting Connected Guide
Thermo Scientific
3 Solvent Line Connections
High-Pressure Connection between the Pump and the Autosampler
Connecting the Solvent Line to the Accela Open Autosampler Injection Valve
The Accela Open Autosampler has a high-pressure Valco™ injection valve (18 500 psi). To
make connections to this valve, use two-piece, stainless steel, compression fittings.
 To connect the high-pressure solvent line to the injection valve
1. Route the stainless steel tubing connected to the LC pump outlet upward through the
access slot on the top right of the pump enclosure.
2. If your LC system includes an Accela detector, route the tubing through the access slots
on the right side of the detector drip tray and the top right of the detector enclosure.
3. Using a two-piece compression fitting, connect the tubing to port 4 of the injection valve
(see Figure 47).
Figure 47. Accela Open Autosampler injection valve with the solvent line from the pump
connected to port 4
2
6
3
1
5
To
the LC column
4
From
the LC pump
4. Using a 1/4 in. open-end wrench, tighten the fitting.
Thermo Scientific
Accela LC System Getting Connected Guide
49
3
Solvent Line Connections
LC Column Installation
LC Column Installation
To install the LC column, follow these procedures:
1. Connecting the LC Column Inlet to the Autosampler Injection Valve
2. Connecting the LC Column Outlet to the Detector Inlet
3. (Accela Autosampler only) Mounting the LC Column to the Column Support
If your LC system has an Accela Autosampler, make sure that the column oven compartment
is at room temperature before you connect the LC column.
CAUTION To prevent personal injury, before you install or remove the LC column, ensure
that the column oven compartment is at room temperature. The column oven
compartment can reach temperatures as high as 95 °C (203 °F). Figure 44 shows the Hot
Surface caution symbols on the column oven surface.
Connecting the LC Column Inlet to the Autosampler Injection Valve
Both the Accela Autosampler and the Accela Open Autosampler have high-pressure Valco
injection valves; however, the valve models differ.
IMPORTANT The connection to the LC column inlet is under high pressure. When you
are operating the Accela UHPLC system at pressures above 276 bar (4000 psi), use
0.005 in. ID × 1/16 in. OD stainless steel tubing and two-piece compression fittings to
make this connection.
To connect the LC column inlet to the autosampler injection valve, go to the appropriate
procedure:
• Connecting the Column Inlet to the Accela Autosampler Injection Valve
• Connecting the LC Column Inlet to the Accela Open Autosampler Injection Valve
50
Accela LC System Getting Connected Guide
Thermo Scientific
3
Solvent Line Connections
LC Column Installation
Connecting the Column Inlet to the Accela Autosampler Injection Valve
For the Accela Autosampler, connect the LC column inlet to port 6 of the injection valve as
described in the following procedure.
 To connect the LC column inlet to port 6 of the autosampler injection valve
1. Using a two-piece compression fitting (see Figure 43 on page 46), connect a length of
0.005 in. ID × 1/16 in. OD, stainless steel tubing to port 6 of the autosampler injection
valve (see Figure 48).
Figure 48. Accela Autosampler injection valve with solvent line connections
20
µL
2
1
3
6
4
Connection to port 6 of the
autosampler injection valve
5
2. Using a two-piece compression fitting (see Figure 43 on page 46), connect the other
end of the 0.005 in. ID × 1/16 in. OD, stainless steel tubing to the LC column inlet.
Connecting the LC Column Inlet to the Accela Open Autosampler Injection Valve
For the Accela Open Autosampler, connect the LC column inlet to port 5 of the injection
valve as described in the following procedure.
 To connect the LC column inlet to port 5 of the autosampler injection valve
Using two compression fittings (see Figure 43 on page 46), do the following:
• Connect a length of 0.005 in. ID × 1/16 in. OD, stainless steel tubing to port 5 of
the autosampler injection valve (see Figure 47 on page 49).
• Connect the other end of the tubing to the LC column inlet.
Thermo Scientific
Accela LC System Getting Connected Guide
51
3
Solvent Line Connections
LC Column Installation
Connecting the LC Column Outlet to the Detector Inlet
The Accela family of LC detectors includes the Accela PDA Detector, the Accela UV/Vis
Detector, and the Accela RI Detector.
The PDA and UV/Vis detectors have LightPipe™ flow cells. For information about how to
connect the inlet and outlet tubing to the LightPipe flow cell and install it in the Accela
detector, refer to the hardware manual for that detector.
Tip Once the mobile phase exits the LC column, it is no longer under high pressure. For
the UV/Vis and PDA detectors, use PEEK fittings and tubing to connect the LC column
outlet to the detector inlet.
 To connect the LC column outlet to the PDA or UV/Vis detector inlet
1. Route the tubing from the inlet port of the detector flow cell as follows:
• For the Accela Open Autosampler, route the tubing downward through the cutout on
the top left side of the detector enclosure.
• For the Accela Autosampler, route the tubing upward through the access slots on the
top right side of the autosampler enclosure and the right side of the detector drip tray.
2. Connect the tubing to the detector inlet as follows:
• For a UV/Vis or PDA detector with a LightPipe flow cell, use a 1/16 in. PEEK fitting
to connect the inlet port tubing (red PEEK, insulated, 0.005 in. ID × 1/16 in. OD)
that is attached to the LightPipe flow cell to the outlet port of the LC column.
• For an RI detector, do one of the following:
–
If the RI detector is the only detector in the LC system, use the 1/16 in. OD,
stainless steel tubing with fittings (supplied in the RI detector accessory kit) to
connect the outlet of the LC column to the IN port of the RI detector.
–
If the system contains both a PDA detector or UV/Vis detector and an RI
detector, use 0.005 in. ID × 1/16 in. OD (red) or 0.010 in. ID × 1/16 in. OD
(blue) PEEK tubing and fittings to connect the outlet of the LightPipe flow cell
to the IN port of the RI detector.
Mounting the LC Column to the Column Support
If your Accela LC system includes an Accela Autosampler, mount the LC column using the
column clamp inside the column oven compartment.
CAUTION To prevent personal injury, before you touch the metal surfaces inside the
column oven compartment, ensure that the column oven compartment is at room
temperature. The column oven compartment can reach temperatures as high as 95 °C
(203 °F). Figure 44 shows the Hot Surface caution symbols on the column oven surface.
52
Accela LC System Getting Connected Guide
Thermo Scientific
3
Solvent Line Connections
LC Column Installation
 To mount the LC column
1. Make sure that the column oven compartment is at room temperature.
2. Adjust the height of the clamp by sliding the clamp up or down the clamp track.
3. To open the clamp, press the tab (see Figure 49) on the right side of the clamp (current
version).
Figure 49. Column clamp and clamp track
Linear column clamp guide
Foam pads inside the column clamp
Tab
4. Mount the LC column and close the clamp.
The compressible foam pads inside the column clamp adjust to the column thickness.
5. Route the outlet tubing through the access slot in the top of the column oven
compartment.
6. Ensure that the inlet tubing is appropriately routed through the access slot at the bottom
of the column oven compartment (see Figure 50).
IMPORTANT When operating the pump at pressures above 276 bar (4000 psi), you
must use stainless steel tubing to connect the autosampler outlet to the LC column
inlet.
Thermo Scientific
Accela LC System Getting Connected Guide
53
3
Solvent Line Connections
LC Column Installation
Figure 50. Column oven compartment with an installed LC column
THERMO
Stainless steel tubing routed
through the cutout in the top of the
column oven compartment
LC column
20
µL
Stainless steel tubing routed
through the access slot in the
autosampler drip tray
7. Close the column oven compartment door.
54
Accela LC System Getting Connected Guide
Thermo Scientific
3 Solvent Line Connections
Connecting the RI Detector Outlet to Waste
Connecting the RI Detector Outlet to Waste
Use the tubing supplied with the RI detector to connect the OUT port to a waste container.
This tubing has an inner diameter of 0.06 inches.
CAUTION Because the flow cell in the Accela RI Detector cannot withstand a
backpressure greater than 517 kPa (75 psi), the detector must be the last component in
the solvent path of the LC system. To avoid damaging the flow cell, do not connect
another detector, backpressure regulator, or narrow-bore tubing to the OUT port of the
Accela RI Detector.
 To connect the OUT port of the RI detector to the waste container
1. Using the 0.060 in. ID × 1/16 in. OD Teflon tubing supplied in the accessory kit,
connect the OUT port of the RI detector to a waste bottle with secondary containment.
2. Route the tubing through the access slot in the detector’s drip tray.
Thermo Scientific
Accela LC System Getting Connected Guide
55
4
Drainage Tubing Connections
The Accela Autosampler, Accela 600 Pump, and Accela 1250 Pump ship with preinstalled
drainage lines between the drip trays. You must install the drain lines from the bottom of the
pump and the drainage manifold on the Accela Autosampler to the solvent waste container.
For the Accela Open Autosampler, you must connect the waste line to a waste container.
Contents
• Accela Autosampler and Accela Pump Drainage Connections
• Accela Open Autosampler Drainage Connections
Accela Autosampler and Accela Pump Drainage Connections
Table 3 lists the part numbers and application of the two sections of drainage tubing for the
Accela Autosampler and an Accela pump.
Table 3. Drainage tubing
Length
Application
Part number
0.9 m (36 in.)
Drains fluid from the bottom of the pump to the
waste bottle (cuff 1 end).
F5034-040
1.2 m (48 in.)
Drains fluid from the drainage manifold of the
autosampler to the waste bottle (cuff 1 end).
F5034-050
 To attach the two precut pieces of drainage tubing
1. Ensure that the drainage port on the bottom of the detector is aligned directly over the
drainage funnel on the top of the autosampler.
2. Attach the 1.2 m (48 in.) piece of convoluted tubing to the drain manifold
(see Figure 51), located in front of the tray compartment:
a. Using pliers, open the zinc spring clamp that is attached to the cuffed end of the
1.2 m (48 in.) length of convoluted tubing.
b. Slip the cuffed end of the tubing onto the drain manifold. To secure the tubing to the
drain manifold, adjust the clamp over the cuff, ensuring that the straight ends of the
clamp face in toward the tray compartment.
Thermo Scientific
Accela LC System Getting Connected Guide
57
4
Drainage Tubing Connections
Accela Autosampler and Accela Pump Drainage Connections
c. Place the uncuffed end of the tubing into an appropriate waste container.
Figure 51. Convoluted tubing secured to the drain manifold with a clamp
Drainage manifold
Zinc clamp
Cuffed end of 48 in.
convoluted tubing
3. Attach the cuffed end of a 0.9 m (36 in.) length of convoluted tubing to the drain port on
the bottom of the pump and place the other end of the tubing into an appropriate waste
container.
4. For a stand-alone LC system (without a mass spectrometer), route the outlet tubing from
the detector through the drain manifold of the autosampler into the 1.2 m (48 in.) length
of convoluted tubing that drains to the waste bottle.
Figure 52 shows all the drainage tubing connected.
58
Accela LC System Getting Connected Guide
Thermo Scientific
4 Drainage Tubing Connections
Accela Autosampler and Accela Pump Drainage Connections
Figure 52. Drainage line connections
Solvent platform
Accela detector
(PDA, UV/Vis, or RI
detector)
Flow cell outlet
tubing
Accela Autosampler
Autosampler funnel
Drainage
manifold
Accela pump
(Accela Pump,
Accela 600 Pump, or
Accela 1250 Pump)
1.2 m (48 in.)
convoluted tubing
Pump funnel
0.9 m (36 in.)
convoluted tubing
Thermo Scientific
Accela LC System Getting Connected Guide
59
4
Drainage Tubing Connections
Accela Autosampler and Accela Pump Drainage Connections
5. Provide secondary containment for the waste container (see Figure 53).
Figure 53. Drainage tubing connected to a waste bottle with secondary containment
ACCELA
PDA Detector
Power
Communication
Run
Lamps
ACCELA
Autosampler
Power
Communication
Run
Temperature
ACCELA
Pump
Power
Communication
Run
Degas
Waste bottle
Secondary
containment
60
Accela LC System Getting Connected Guide
Thermo Scientific
4 Drainage Tubing Connections
Accela Open Autosampler Drainage Connections
Accela Open Autosampler Drainage Connections
For an LC system with an Accela Open Autosampler, the pump and detector reside under the
autosampler table. If the system contains two pumps, the pumps reside under the autosampler
table and the detector resides next to the autosampler table.
The dynamic load and wash (DLW) option is a standard component of the Accela Open
Autosampler. This option includes two micro pumps, a wash station, solenoid valve, and
syringe set.
 To connect the drainage system for an LC system with an Accela Open Autosampler
1. Make sure that the waste line is connected between port 2 of the autosampler injection
valve and the upper waste port on the front of the DLW wash station.
2. Do one of the following:
• If your autosampler has only one injection valve, make sure that the lower waste port
on the front of the wash station is closed with a dummy plug.
• If your autosampler has two injection valves, make sure that the second injection
valve is connected to the lower waste port on the front of the wash station.
3. Place the free end of the drainage tubing that is connected to the bottom of the wash
station into a waste container that is located below the height of the injection valve. Verify
that the waste liquid flows into the waste container without restriction.
4. Place the free end of the tubing connected to the detector flow cell outlet into the waste
container.
5. Place the free end of the drainage tubing connected to the module on the bottom of the
stack into the waste container.
Thermo Scientific
Accela LC System Getting Connected Guide
61
A
System Connection Parts and Kits
This appendix provides information about the parts that you use to make the system contact
closure and plumbing connections.
Contents
• Accela System Kit
• Solvent Interconnect Kit
• Accela Open Autosampler Accessories
Accela System Kit
Table 4 lists the parts supplied in the Accela System Kit (November 2010 or later). With the
exception of the Accela Open Autosampler interconnect and adapter cables, this kit contains
the cables, tubing, and fittings for connecting the Accela LC system modules.
Note Accela Accessory Kits shipped before November 2010 do not contain the 0.188 in.
length ferrules.
Table 4. Accela System Kit (P/N 60057-60060) (Sheet 1 of 2)
Description
Part number
Quantity
5-port Ethernet switch, 5 port, 10/100 Base-T, 100 - 240 V
00825-01-00024
1
Ethernet cable with two ferrites
97355-98006
1
System interconnect cable
(7-connector cable for an Accela UHPLC system with an Accela Autosampler)
60053-63034
1
UHP filter cartridge, 0.5 μm particle size, Teflon
00109-99-00020
1
Solvent bottle holder
(solvent platform for Accela system that contains the Accela Autosampler)
60057-60014
1
Solvent bottle, 1 liter
1413-0430
4
Solvent bottle caps (outer portion)
A0343-010
4
Solvent bottle cap adapter (inner portion)
A3191-010
4
Solvent bottle label set
A4158-020
1
Thermo Scientific
Accela LC System Getting Connected Guide
63
A
System Connection Parts and Kits
Accela System Kit
Table 4. Accela System Kit (P/N 60057-60060) (Sheet 2 of 2)
Description
Part number
Quantity
Tubing, stainless steel, 0.005 in. ID × 1/16 in. OD, 10 cm length (to connect
00301-01-00008
3
Marker letter A, clip on for 1/8 in. OD tubing
608250001
1
Marker letter B, clip on for 1/8 in. OD tubing
608250002
1
Marker letter C, clip on for 1/8 in. OD tubing
608250003
1
Marker letter D, clip on for 1/8 in. OD tubing
608250004
1
Tubing, stainless steel, 0.005 in. ID × 1/16 in. OD, 20 cm length
(to connect the Accela Pump outlet to the inlet of the Accela Autosampler heat
exchanger)
00301-01-00009
1
Tubing, stainless steel, 0.005 in. ID × 1/16 in. OD, 26 cm length
(to connect the Accela 600 Pump or the Accela 1250 Pump outlet to inlet of the
Accela Autosampler heat exchanger)
00028-01-00035
1
Tubing, stainless steel, 0.005 in. ID × 1/16 in. OD, 60 cm length
(to connect the Accela pump (all models) outlet to the Accela Open Autosampler
injection valve)
00028-01-00039
1
Ferrule, 0.188 in. length, for 1/16 in. OD tubing, stainless steel
00101-18187
12
Ferrule, Valco zero dead volume for 1/16 in. OD tubing, 10 000 psi rating
00101-18122
12
Nut, compression, 0.45 in. length, for 1/16 in. OD tubing, 10-32 thread, stainless 2522-1880
steel
Vial kit, clear vials with 1.8 mL capacity, black screw-on caps, 100 vials and caps
per package
A4954-010
12
2
Instrument control software, LC Devices (for use with the Xcalibur data system and 60257-62005
several other Thermo Scientific mass spectrometry applications)
1
Instrument control software, Thermo pumps add-on for the ChromQuest 5.0 data CHROM-93034
system
1
Instrument control software, Accela detectors add-on for the ChromQuest 5.0 data CHROM-98036
system
1
Instrument control software, Accela Open Autosampler add-on for the
ChromQuest 5.0 data system
CHROM-98037
1
Data system independent instrument control software for the Accela Open
Autosampler (PAL loader and object manager)
CHROM-98035
1
64
Accela LC System Getting Connected Guide
Thermo Scientific
A
System Connection Parts and Kits
Solvent Interconnect Kit
Solvent Interconnect Kit
Table 5 lists the parts supplied in the Solvent Interconnect Kit (P/N F5050-010). This kit
contains the solvent reservoir bottles and low-pressure, 1/8 in. OD solvent lines for the LC
system. This kit also contains the Super Flangeless fittings used to connect the solvent lines to
the inlet ports of the degassing unit in the Accela Pump and the Teflon solvent filters used to
terminate the solvent lines.
Table 5. Solvent Interconnect Kit (P/N F5050-010)
Description
Part number
Quantity
Solvent bottle caps and solvent bottle cap adapters
A0343-010
4
Solvent bottle cap adapters
A3191-010
4
10 μm particle size, Teflon solvent filters
A4258-010
4
FEP tubing (1/8 in. OD × 1/16 in. ID)
3219-2004
6 m (20 ft)
Super Flangeless fittings for 1/8 in. OD tubing
(see Figure 35 on page 40)
00101-18225
4
Super Flangeless ferrule for 1/8 in. OD tubing
00101-18017
4
Solvent bottle label set
A4518-020
N/A
Clip-on markers used to label the solvent lines: A, B,
C, and D
608250001608250004
1 each
Accela 600 and Accela 1250 Pump Inlet Tubing Kit
The tubing kit for the Accela 600 and Accela 1250 pumps (P/N 60157-62008) contains
four solvent reservoir lines. Each solvent line consists of a 3 m (10 ft) length of
1/8 in. OD × 1/16 in. ID, FEP tubing with a 1/4 in. × 28 PEEK flangeless fitting at one end
and a 20 μm particle size, stainless steel filter at the other end.
Thermo Scientific
Accela LC System Getting Connected Guide
65
A
System Connection Parts and Kits
Accela Open Autosampler Accessories
Accela Open Autosampler Accessories
Table 6 lists the parts that you use to make the connections between the Accela Open
Autosampler, the other Accela UHPLC modules, and a Thermo Scientific mass spectrometer.
Table 6. System connection parts for the Accela Open Autosampler
Description
Location
Part number
Interconnect (contact closure) cable that
synchronizes the run signals between the
autosampler, an Accela 600 or 1250 Pump,
and an LTQ Series or MSQ mass
spectrometer
Supplied with the
Accela Open
Autosampler
60157-63024
Detector adapter (trigger) cable for TSQ
Series and Exactive mass spectrometers and
Accela detectors
Supplied with the
Accela Open
Autosampler
60157-63026
Accela Pump adapter (contact closure) cable
Available by special
order
60157-63022
Tubing, stainless steel,
0.005 in. ID × 1/16 in. OD,
0.60 m (2 ft) length
Supplied with the
Accela Open
Autosampler
N/A
Ferrule, 0.188 in. length, for 1/16 in. OD
tubing, stainless steel
Nut, compression, 0.43 in. length,
for 1/16 in. OD tubing, 10-32 thread,
stainless steel
00101-18187
Supplied with the
Accela Open
Autosampler
N/A
Tip To connect the solvent line between the pump outlet and the autosampler injection
valve, you can also use the stainless steel, compression nut in the Accela Accessory Kit.
66
Accela LC System Getting Connected Guide
Thermo Scientific
I
Index
A
C
Accela 600 or 1250 Pump
data system computer connection 27
Inlet Tubing Kit 42
interconnect cable connection 8
solvent reservoir bottle assembly 42
solvent reservoir line connections 44
Accela Autosampler
data system connection 24
drain manifold 57
interconnect cable connection 8
LC column connection 51
solvent line connection to the heat exchanger inlet 47
weight 4
Accela detector
contact closure connection
Accela Autosampler 8
Accela Open Autosampler 18
data system connection 24
inlet tubing connection 52
Accela Open Autosampler
adapter cable for detector 18
interconnect cable 15
line power connections 31
wash station 61
weight 5
Accela Pump
contact closure connection
Accela Autosampler 8
Accela Open Autosampler 22
data system computer connection 27
solvent reservoir bottle assembly 39
solvent reservoir line connections 41
Accela System Kit 63
cables
Accela Autosampler system interconnect cable 8
Accela Open Autosampler serial cable 28
Accela Open Autosampler system interconnect cable 15
Accela Pump adapter cable 22
detector adapter cable 18
Ethernet 24
LCQ Series MS detector adapter cable 11
LTQ Series MS detector adapter cable 12
TSQ Series MS detector adapter cable 13
USB for pump 27
compliance
FCC viii
regulatory iii
WEEE ix
contact closure cables
Accela Autosampler
LCQ Series adapter cable 11
LXQ/LTQ adapter cable 12
system interconnect cable 8
TSQ Series adapter cable 13
Accela Open Autosampler
Accela Pump adapter cable 22
LC detector or TSQ/Exactive adapter cable 18
system interconnect cable 15
Thermo Scientific
D
degassing unit, built-in 2
DLW wash station 61
drain manifold 57
dual-pump system, contact closure to the Accela Open
Autosampler 15
Accela LC System Getting Connected Guide
67
Index: E
E
O
electromagnetic compatibility viii
eluting pump, contact closure connection with an Accela
Open Autosampler 15
EMC compliance iv
Ethernet cables, connecting 24
OUT port, RI detector 55
F
FCC compliance viii
fittings
for 1/16 in. OD stainless steel tubing 46
Super Flangeless 40
P
ping command 26
plugs
international 29
NEMA 29
power cords 29
pump
See Accela Pump or Accela 600 or 1250 Pump
R
I
injection valve
Accela Autosampler 51
Accela Open Autosampler 51
interconnect cables, system 7
regulatory compliance iii
RI detector
OUT port 55
pressure rating 3
principle of operation 2
K
S
kits
Accela 600 or 1250 Pump Inlet Tubing 42
Accela System 63
Solvent Interconnect 65
Wash Bottle 37
MSQ Plus MS detector, contact closure connection
Accela Autosampler 10
Accela Open Autosampler 16, 19
safety standards iii
Solvent Interconnect Kit 65
solvent lines
Accela 600 or 1250 Pump solvent reservoir lines 44
Accela Pump solvent reservoir lines 41
between the pump and the autosampler 47
needle tubing 38
wash bottle solvent line 37
solvent reservoir bottles
for Accela 600 or 1250 Pump 42
for Accela Pump 39
solvent waste
from LC system 57
from the RI detector OUT port 55
static mixer connections, Accela 600 or 1250 Pump 45
syringe valve
needle tubing connection 38
wash bottle tubing connection 37
system interconnect cables
Accela Autosampler 8
Accela Open Autosampler 14
N
T
needle tubing, connecting 38
TSQ Series MS, contact closure
Accela Autosampler 13
Accela Open Autosampler 18
L
LC column
Accela Autosampler connection 51
Accela Open Autosampler connection 51
LCQ Series MS detector, contact closure connection 11
line power, connecting modules to 29
LTQ Series MS, contact closure connection
Accela Autosampler 12
Accela Open Autosampler 16
M
68
Accela LC System Getting Connected Guide
Thermo Scientific
Index: U
tubing
access slots for 35
between the pump and the autosampler 45
color-coded band 45
drainage 57
for RI detector OUT port 55
needle tubing 38
precut lengths 46
solvent reservoir lines 38
wash bottle tubing 37
U
USB cable for pump 27
V
valves
injection, Accela Autosampler 51
injection, Accela Open Autosampler 49
proportioning 2
syringe 37
W
Wash Bottle Kit 37
wash bottle, syringe valve connection 37
wash station, Accela Open Autosampler 61
waste container
RI detector OUT port connection 55
system drainage and solvent waste connections 57
WEEE compliance ix
weight
Accela Autosampler 4
Accela Open Autosampler 5
Thermo Scientific
Accela LC System Getting Connected Guide
69
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertisement