SANYO MCO 20AIC CO2 Incubator INSTRUCTION MANUAL
Below you will find brief information for CO2 Incubator MCO 20AIC. The MCO-20AIC CO2 incubator is designed for cell culture applications. It features precise temperature and CO2 control, as well as a built-in UV lamp for sterilization . The unit is also equipped with an automatic calibration function for the CO2 sensor. The system is easy to use and features alarms for various conditions.
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INSTRUCTION MANUAL CO2 Incubator MCO-20AIC Note: 1. No part of this manual may be reproduced in any form without the expressed written permission of SANYO. 2. The contents of this manual are subject to change without notice. 3. Please contact SANYO if any point in this manual is unclear or if there are any inaccuracies. SANYO Electric Biomedical Co., Ltd. All rights reserved. Printed in Japan. CONTENTS PRECAUTIONS FOR SAFE OPERATION P. 2 CAUTIONS FOR USAGE P. 6 INCUBATOR COMPONENTS P. 8 INSTALLATION P. 11 BEFORE COMMENCING OPERATION P. 13 OPERATING INSTRUCTIONS P. 16 ALARM & SAFETY FUNCTIONS P. 20 ROUTINE MAINTENANCE P. 22 CALIBRATION P. 26 TROUBLESHOOTING P. 27 ENVIRONMENTAL CONDITIONS P. 28 DISPOSAL OF UNIT P. 28 STACKED MODULE P. 29 SPECIFICATIONS P. 31 PERFORMANCE P. 32 SAFETY CHECK SHEET P. 33 1 PRECAUTIONS FOR SAFE OPERATION It is imperative that the user complies with this manual as it contains important safety advice. Items and procedures are described so that you can use this unit correctly and safely. If the precautions advised are followed, this will prevent possible injury to the user and any other person. Precautions are illustrated in the following way: WARNING Failure to observe WARNING signs could result in a hazard to personnel possibly resulting in serious injury or death. CAUTION Failure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property. Symbol shows; this symbol means caution or warning. this symbol means an action is prohibited. this symbol means an instruction must be followed. this symbol means UV caution. this symbol means attention or refer to document. this symbol means hot surface. This symbol means earth. this symbol means power switch “ON”. this symbol means power switch “OFF”. Be sure to keep this manual in a place accessible to users of this unit. 2 PRECAUTIONS FOR SAFE OPERATION WARNING Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to rain water. Only qualified engineers or service personnel should install the unit. unqualified personnel may cause electric shock or fire. The installation by Install the unit on a sturdy floor. If the floor is not strong enough or the installation site is not adequate, this may result in injury from the unit falling or tipping over. Never install the unit in a humid place or a place where it is likely to be splashed by water. Deterioration of the insulation may result which could cause current leakage or electric shock. Never install the unit in a flammable or volatile location. This may cause explosion or fire. Never install the unit where acid or corrosive gases are present as current leakage or electric shock may result due to corrosion. Use a dedicated power source as indicated on the rating label attached to the unit. Remove dust from the power supply plug before inserting in a power source. A dusty plug or improper insertion may cause a hazard. Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet is not grounded (earthed), it will be necessary to install a ground by qualified engineers. Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such grounding may cause electric shock in the case of an incomplete circuit. Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are connected correctly and are not liable to become disconnected. Ensure that the gas pressure is set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure may result in leakage of CO2 gas. Elevated level of CO2 gas can be hazardous to health and may lead to asphyxiation and risk of death. Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet for inner air circulation. This may cause electric shock or injury by accidental contact with moving parts. Never store volatile or flammable substances in this unit. This may cause explosion or fire. 3 PRECAUTIONS FOR SAFE OPERATION WARNING As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity of the equipment. It is important that you assess the work site to ensure there is suitable and sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a safe environment must be considered. These may include atmosphere monitoring and warning devices. Keep proper gas pressure to avoid gas leak. Ventilate a room air occasionally when using CO2 gas for control. The gas density will increase in an enclosed small room and high level of gas density can be hazardous to health. In addition, avoid inhaling the chamber air directly when opening the door if CO2 gas is used. Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densité CO2 de l’air peut s’élever et peut être nocif aux humains. Évitez d’aspirer l’air provenant de l’intérieur de l’appareil quand vous ouverz la porte. Use this unit in a safe area if using poisonous, harmful or radioactive substances. Improper use may be harmful to your health or the environment. Never expose the eyes directly to UV light as UV light can cause permanent damage to eyes. Never remove cover when UV light is ON. Hazardous UV light. Do not press door switch. Disconnect the power supply to the unit prior to any repair or maintenance in order to prevent electric shock or injury. Ensure you do not inhale or consume medication or aerosols from around the unit at the time of maintenance. These may be harmful to your health. Never splash water directly onto the unit as this may cause electric shock or short circuit. Never disassemble, repair, or modify the unit yourself. Any such work carried out by an unauthorized person may result in fire or injury due to a malfunction. Disconnect the power supply plug if there is anything wrong with the unit. Continued abnormal operation may cause electric shock or fire. If the unit is to be stored unused in an unsupervised area for an extended period, ensure that children do not have access and that doors cannot be closed completely. The disposal of the unit should be undertaken by appropriate personnel. Remove doors to prevent accidents such as suffocation. Prepare a safety check sheet when you request any repair or maintenance for the safety of service personnel. 4 PRECAUTIONS FOR SAFE OPERATION CAUTION Select a level and sturdy floor for installation. This precaution will prevent the unit from tipping. Improper installation may result in water spillage or injury from the unit tipping over. Connect the unit to a power source as indicated on the rating label attached to the unit. Use of any other voltage or frequency other than that on the rating label may cause fire or electric shock. Fix the shelves securely. Incomplete installation may cause injury or damage. When removing the plug from the power supply outlet, grip the power supply plug, not the cord. Pulling the cord may result in electric shock or fire by short circuit. Never damage or break the power supply plug or cord. Do not use the supply plug if its cord is loose. This may cause fire or electric shock. Do not touch any electrical parts such as the power supply plug or any switches with a wet hand. This may cause electric shock. Do not put a container with water or heavy articles on the unit. It may cause injury if the articles fall. Current leakage or electric shock may result form deterioration of insulation by spilled water. Do not climb onto the unit and do not put articles on the unit. This may cause injury by tipping or damage to the unit. Never lean or press on the glass. Intentional force may cause injury if the glass breaks. Do not lean on the door. This may cause injury, current leakage, or electric shock if the unit tips over or door becomes detached. Disconnect the power supply plug before moving the unit. Take care not to damage the power cord. A damaged cord may cause electric shock or fire. Empty the humidifying pan completely before moving the unit. Spilled or splashed water may cause current leakage or electric shock. Be careful not to tip over the unit during movement to prevent damage or injury. Disconnect the power plug when the unit is not used for long periods. Do not put the packing plastic bag within reach of children as suffocation may result Take care of the inside of the outer door. It may get hot. 5 CAUTIONS FOR USAGE 1. Install in an appropriate environment With an automatic calibration function, the CO2 sensor is calibrated by utilizing air from the local environment. Place the unit in an environment which will reflect normal atmospheric conditions. 2. 5oC higher than the ambient temperature The chamber temperature must be at least 5oC higher than the ambient temperature. For example, the chamber temperature is set to 37oC, the ambient temperature must be less than 32oC. Ensure the ambient temperature is within the desired range. 3. Do not subject to direct air flow Do not place the unit in the direct air flow from an air conditioning system. conditioning system may cause condensation and lead to possible contamination. Cool air from an air 4. Always keep the chamber clean The Petri dishes or bottles for culturing may cause contamination in the chamber. Clean the containers before storing them in the chamber. 5. Allow adequate space between the cultures When storing cultures in the chamber, keep the Petri dishes or bottles containing the cultures sufficiently apart from each other to allow adequate air circulation. Inadequate space may result in uneven temperature distribution and CO2 concentration in the chamber. 6. Always shut the inner door Shut the inner door completely, and then shut the outer door. If the inner door is not closed completely, even if the outer door is closed, the unit will fail to exhibit its maximum performance. Ensure you close the doors gently. Robust closing may cause spillage of medium, incomplete closing, or damage of gasket. Before opening the inner door, check that the UV light is OFF. 7. Keep the inside panels dry To protect the inside of the unit from contamination, the inside panels should always be kept dry. If water is spilled from the humidifying pan or if the door is kept open for a long period, condensation will form on the panels, allowing bacteria or fungi to breed. If this occurs, wipe away the water with a dry sterile gauze. Particularly, if the medium is spilled, wipe it up immediately and sterilize the area. 8. Fill the humidifying pan with sterile distilled water Always use sterile distilled water to fill the pan. The RH PAN light on the control panel flashes when the water level is low. Refill the sterile distilled water to the pan when the RH PAN light blinks. Note that when low temperature water is poured, the chamber temperature drops significantly. Clean the pan once a month. 9. Humidifying pan and pan cover The humidifying pan and pan cover prevent the UV light from escaping. Make sure they are installed even if you do not need humidity. Install the pan cover correctly since it affects the temperature distribution and humidity recovery in the chamber. 6 CAUTIONS FOR USAGE 10. Stored materials Never place acid or alkaline materials or materials that release corrosive gas in the chamber. Such materials can cause failure resulting from discoloration or corrosion. 11. Alarm Always investigate the cause and fix the alarm condition immediately when the alarm is activated. Refer to page 20 for alarm details. 12. Never use CAL key Never use CAL key during normal operation because the calibration mode is started by pressing this key. Wrong operation of this key will give an adverse effect on the basic performance of the unit. If you press this key accidentally, do not press any keys on the control panel for 90 seconds, after which the unit returns to the display mode automatically. 13. UV lamp The UV lamp is OFF when the outer door is opened as it is operated to outer door switch. Check the UV lamp is ON with the inner door closed once a month by activating the outer door switch. If the lamp is not ON, the lamp needs to be replaced. Contact Sanyo sales representative or agent. The UV lamp is located in the duct. Take care not to damage the lamp at the time of installation/removal of attachments or humidifying pan. Note: UV light is harmful to the eyes. Never turn on the UV lamp with the inner door opened. 14. Notice of recommended replacement of UV lamp This unit is provided with a function to notify the recommendation of UV lamp replacement when the accumulated ON time of UV lamp is over about 1,000 hours. The blink of the UV indicator on the control panel recommends the replacement of UV lamp. For the replacement, contact Sanyo sales representative or agent. 15. If not used for a long period When the unit is not used for a long period, dispose of the water in the humidifying pan and completely remove any moisture in the chamber completely. Check that the chamber is completely dry before closing the doors. 7 INCUBATOR COMPONENTS 1 6 12 7 8 9 (inside) 4 2 13, 15, 16 (inside) 10 21 19 5 Switch cover 14 3 17 18 11 20 Rear right side Rear left side 8 INCUBATOR COMPONENTS 1. Outer door: Sticks to frame with magnetic seal. Door heater is installed in the door panel. The heater eliminates moisture on the inner door. The door opening is reversible. Contact Sanyo representative or agent to change the door hinge from left to right or vice versa. 2. Inner door: Made of tempered glass. Avoid excessive impact on the glass. 3. Leveling foot: Used for leveling the unit. 4. Tray: Can be pulled toward you. 5. Tray support: Can be removed by lifting the front side and pulling toward you. 6. Side support: Right and left side supports can be removed for disinfection. See page 23 and 24. 7. Top duct: Inlet of circulating air. Removable. 8. Rear duct: Flow path for circulating air. Removable. 9. Fan (inside the rear duct): Made from polypropylene resin. Can be disinfected by an autoclave. 10. Sample air outlet: This also functions as an internal gas outlet. Normally, cover this outlet with the sample air outlet cap. 11. Connecting port for CO2 gas pipe (rear side): When an optional component MCO-21GC (cylinder changeover accessory) is installed, both A and B are available. If MCO-21GC is not used, only A is available. Refer to page 14 for gas cylinder connection. Ensure that the gas pressure is set at 0.03MPaG (0.3kgf/cm2G, 4.3psiG). 12. Door switch: Detects the door opening/closing and stops the circulating fan and electromagnetic valve for CO2 when door is open. UV lamp is also deactivated by door opening. 13. Humidifying pan: Use sterile distilled water to fill the pan. Install the pan properly so that it can be covered with the pan cover. 14. Humidifying pan cover: Prevents UV light being exposed to the chamber. When filling the pan, lift the front side and take out the pan. See page 23 for details. 15. UV lamp: Sanyo UV lamp does not generate ozone. Never look at the UV light directly. For replacement, contact Sanyo representative or agent. 16. Water level sensor for humidifying pan: Located at the rear of the chamber and detects the water level in the humidifying pan. Never use excessive force on the sensor during maintenance. 17. Remote alarm terminal: Refer to page 21. 18. Access port: When not in use, cap with the rubber cap on both outside and inside. 19. Power switch: Main switch of the unit. Also functions as an over-current breaker (10A). The switch is covered by a switch cover to prevent the accidental push. To turn on or off the switch, remove the switch cover by loosening the screw. 20. Glow lamp: For UV lamp (model; FG-7P) 21. Sample air outlet cap: Always attach this cap except at the time of using of sample air outlet. 9 INCUBATOR COMPONENTS Control panel and keypad 3 2 1 8 9 4 5 6 11 12 7 13 10 17 o 16 15 14 1. Digital temperature indicator (TEMPERATURE C): Normally, this indicator shows the chamber temperature. In the setting mode, it shows the set value of the chamber temperature. If the self diagnostic function detects any abnormality, an error code will be displayed. 2. Heater lamp (HEAT): This lamp lights when the heater is energized. 3. UV indicator (UV): This lamp lights when the UV lamp is ON. The blink of this indicator recommends the replacement of UV lamp. See page 17 for the details. 4. Door lamp (DOOR): This lamp lights when the outer door is open. 5. Water level alarm lamp (RH PAN): This lamp flashes when the water in the humidifying pan is less than approximately 1 liter. 6. Upper limit regulator: This regulator is used to set the upper temperature limit. 7. Over heat lamp (OVER HEAT): This lamp lights when the chamber temperature reaches the upper limit set value. 8. CO2 inject lamp (INJECT): This lamp lights when CO2 gas is being injected. 9. Digital CO2 density indicator (CO2 %): Normally, this indicator shows the CO2 concentration in the chamber. In the setting mode, it indicates the set value of the CO2 concentration. 10. Set key (SET): Pressing this key to enter the setting mode, and the digits to be set will flash. 11. Calibration key (CAL): By pressing this key for approximately 5 seconds, the unit enters calibration function mode. Used to change the UV lamp ON period. 12. Alarm buzzer stop key (BUZZER): Press this key to silence the buzzer when the alarm operates and the buzzer sounds. The remote alarm also stops by pressing this key. Press it once again to reactivate the buzzer. 13. CO2 gas supply line indicator (A/B): The lamp for the supply line currently in use lights up provided that MCO-20GC changeover accessory is installed. This will flash when either gas cylinder is empty. 14. CO2 gas supply line switching key : This key to select CO2 gas supply line is available only when an automatic cylinder changeover MCO-21GC (option) is installed. When one CO2 cylinder is empty, the CO2 is supplied by the other cylinder automatically. But if necessary, by pressing this key, the supply line can be switched between A and B manually. 15. Enter key (ENT): Pressing this key memorizes the set value in the controller. 16. Numerical value shift key ( ): Pressing this key in the setting mode causes the numerical value to shift. In key lock mode, pressing this key makes key lock ON or OFF. 17. Digit shift key ( ): Pressing this key in the setting mode causes the changeable digit to shift. Pressing this key more than 5 seconds enters key lock mode. See page 18 for the key lock. 10 INSTALLATION Installation site To operate this unit properly and to obtain maximum performance, install the unit in a location with the following conditions: Note: The ambient temperature must be at least 5oC lower than the set temperature. 1. A location not subjected to direct sunlight or direct air flow from an air conditioner 2. A location with clean air and adequate ventilation (Small and sealed room is not recommended.) WARNING If the unit is used in a small confined room, the CO2 density level in the air could rise and may be harmful to humans. 3. A location away from heat generating sources 4. A location with a sturdy and level floor WARNING Install the unit on a sturdy floor. If the floor is not strong enough or the installation site is not adequate, this may result in injury from the unit falling or tipping over. Select a level and sturdy floor for installation. This precaution will prevent the unit from tipping. Improper installation may result in water spillage or injury from the unit tipping over. 5. A location without flammable or corrosive gas WARNING Never install the unit in a flammable or volatile location. This may cause explosion or fire. Never install the unit where acid or corrosive gases are present as current leakage or electric shock may result due to corrosion. 6. A location not prone to high humidity WARNING Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to rain water. Never install the unit in a humid place or a place where it is likely to be splashed by water. Deterioration of the insulation may result which could cause current leakage or electric shock. 11 INSTALLATION Prevent contamination To prevent contamination of the chamber, select an appropriate location for installation as well as ensuring the complete disinfection of the chamber components. 1. Avoid hot and humid location Avoid location with high temperature and/or humidity as the presence of bacteria in the air is greater than in the normal environment. 2. Avoid drafty location and location with many passers-by Avoid locations near doors, air conditioners, fans, etc., where slight breezes can facilitate the entry of bacteria into the chamber. 3. Installation in a sterile room To promote efficient cultivation, it may be best to install the unit in a sterile room. 4. Use clean containers Contamination is often caused by containers such as Petri dishes or bottles stored in the chamber introducing contamination. Always keep the containers clean. Installation 1. Remove the packaging materials and tapes Remove all transportation packaging materials and tapes. Open the doors and ventilate the unit. If the outside panels are dirty, clean them with a neutral detergent and wipe it up with a wet cloth. 2. Adjust the leveling feet Extend the leveling feet by rotating them counterclockwise so they contact the floor or bench. Ensure the unit is level. 3. Fix the unit Two fixtures are attached to the rear of the frame. Fix the frame to the wall with these hooks and rope or chain. 4. Ground (earth) WARNING Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet is not grounded, it is necessary to install a ground by qualified engineers. Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such grounding may cause electric shock in the case of an incomplete circuit. 12 BEFORE COMMENCING OPERATION Sterilizing of chamber and attachments Before first start-up of the unit, the chamber and internal attachments should be cleaned and sterilized as follows. Note: Take care not to damage the UV lamp or water level sensor at the time of removal or replacement of attachments. Do not clean the inside of the unit with a solution of disodium chlorate or other halogen-based solution because this may cause corrosion of metal surfaces. 1. Take out all attachment such as trays, humidifying pans, etc, from the chamber. Refer to page 23 and 24. 2. Clean all of the attachments with neutral detergent and then wash out the detergent with distilled water. 3. Wipe the attachments with a gauze containing alcohol for sterilization and then wipe off with a dry gauze. 4. Wipe the inside wall of the chamber with a gauze containing alcohol for sterilization and then wipe off with a dry gauze. 5. Replace all attachments in the chamber. Note: Always insert the fan on the motor shaft surely. Improper insertion may cause poor performance. 6. Fill the humidifying pan with sterile distilled water. 13 BEFORE COMMENCING OPERATION Connection of CO2 gas cylinder WARNING Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are connected correctly and are not liable to become disconnected. Ensure that the gas pressure is set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure may result in leakage of CO2 gas. Elevated level of CO2 gas can be hazardous to health and may lead to asphyxiation and risk of death. Use a liquefied CO2 gas cylinder, not a siphon (dip tube) type. The CO2 gas should be 99.5% or more pure. Install a pressure regulator on the cylinder. Use a regulator rated at 25MPaG (250kgf/cm2G, 3600psiG) on the primary side and 0.2MPaG (2.0kgf/cm2G, 30psiG) on the secondary side. Use the following procedure to attach the incubator to the cylinder: 1. Using the gas supply pipe provided, connect the pressure regulator to the CO2 inlet located at the rear left hand side of the CO2 incubator. 2. Set the CO2 pressure on the secondary side to 0.03MPaG (0.3kgf/cm2G, 4.3psiG). Excessive pressure may cause disconnection of internal pipes inside the CO2 incubator which will result in leakage of CO2 gas into the atmosphere. Elevated level of CO2 gas can be hazardous to health and may lead to asphyxiation and risk of death. 3. Check that no gas is leaking at any point where the pipe connects with the CO2 regulator or the CO2 incubator. Note: The incubator, including the gas supply pipes and services must be examined at frequent intervals to ensure they are safe. Ensure that items such as pipes are replaced if there is any sign of deterioration. 14 BEFORE COMMENCING OPERATION Automatic switcher of CO2 gas supply line An automatic switcher of CO2 gas supply line (MCO-21GC) is available as an optional accessory. This kit switches the gas supply line when one CO2 gas cylinder is empty. Note: The installation of MCO-21GC should be implemented by a qualified service personnel. After attachment of MCO-21GC, do the following: 1. Connect a CO2 gas pipe to port A and B respectively. A connecting port for CO2 gas pipe is located on the left back of the unit. (See page 9). 2. Connect a CO2 gas cylinder provided with a gas pressure regulator to each gas pipe. See page 14, for the connection of the gas cylinder. 3. Open the valve of each gas cylinder. 4. Check that the CO2 gas supply line indicator on the control panel is lit by pressing the switching key. 5. Select a CO2 gas supply line (A or B). 6. When one cylinder is empty, the indicator is flashed, buzzer sounds, and “E01” and current chamber temperature are displayed alternately on the control panel while the gas supply line is switched to other one. To silence the buzzer, press the buzzer stop key (BUZZER). 7. Replace the empty CO2 gas cylinder. Note: Exercise caution when handling empty CO2 gas cylinders as some gas can be still be left in the cylinder. This kit MCO-21GC detects that no more CO2 gas exists in a cylinder when the CO2 density in the chamber is not increased for a while after opening of CO2 gas valve in the unit and switches the gas supply line. The switching of supply line can be caused by some other reasons; blocking or restricting of gas tube, reduction of CO2 gas pressure, or improper opening of CO2 gas cylinder, in spite of gas quantity in the cylinder. Therefore, always check the gas quantity in the cylinder prior to disconnection. 15 OPERATING INSTRUCTIONS Set of chamber temperature and CO2 density Table below shows the basic procedure for setting the chamber temperature and CO2 density. The upper limit alarm temperature setting is also shown in the table. Perform key operations in the sequence indicated in the table. The example in the table is based on the assumption that the desired temperature is 37oC and CO2 density is 5%. Note: The unit is set at the factory so that the chamber temperature is 37oC and CO2 control is 0%. Allow at least 4 hours until the next setting after setting of desired chamber temperature and setting CO2 density to 0%, at the time of first start-up or start-up after no use for long term. Basic operation sequence (Example: Description of operation 1 2 3 Key operated ---- Turn the power switch ON. Press SET key. By pressing key and the figure to 37.0. Chamber temperature; 37oC, CO2 density; 5%) SET Press ENT key. ENT 5 By pressing key and the figure to 05.0. 6 Press ENT key. 7 Adjust upper alarm temperature set knob so that the alarm temp. is 1oC higher than chamber temp. 8 Press ENT key. The left digit is flashed. When pressed, the changeable digit is shifted. When pressed, the figure of settable digit increases. key, set 4 Indication after operation The current chamber temperature is displayed in temperature indicator. key, set ENT Set temperature is memorized. Left digit in CO2 density indicator is flashed. When pressed, the changeable digit is shifted. When pressed, the figure of settable digit increases. Set CO2 density is memorized. In CO2 density indicator, HI is displayed. In temperature indicator, 38.0 is displayed. ENT This is the end of set mode and the indicators display current temperature and CO2 density. Note: In each set mode, the indicator returns to the current temperature and CO2 density display mode automatically when 90 seconds has passed without any key operation. In each set mode, if the change of the setting is not necessary, pressing SET key skips to next set mode. When the CO2 density is set to 00.0, the control is OFF regardless of chamber density. The upper limit alarm temperature set value will change when the set knob is turned even if the unit is not in set mode, because the alarm circuit is an independent circuit. 16 OPERATING INSTRUCTIONS UV lamp A UV lamp is located inside the rear duct to sterilize the water in the humidifying pan and air circulating in the chamber. Following shows precautions and instructions about the UV lamp. * The UV light is exposed only to the inside of the duct and the humidifying pan cover when all chamber components are installed properly. * During cultivation, ensure all components are located adequately and never turn on the UV light without the humidifying pan cover. * Even if the unit is operating without turning on the UV lamp, the humidifying pan cover should be installed properly. An operation without pan cover will affect the temperature distribution and humidify recovery. * Even if the unit is operated without humidifying, the humidifying pan and the pan cover should be installed properly. * The UV lamp is turned off when the outer door is opened during ON period. * When checking the UV lamp operation, open the outer door and push the door switch with the inner door closed. * The UV lamp is ON for a predetermined period after the outer door is closed. The period is factory set at 5 minutes. Change the setting as necessary as shown in the table below. * The UV lamp is ON for a predetermined period every 12 hours when the outer door is not opened more than 12 hours continuously. The predetermined period can be changed by following the procedure. * The recommended timing of lamp replacement (the ratio of UV output is less than 70% of initial value) is when the accumulated ON time is over about 1,000 hours. The blink of the UV indicator when the UV lamp is OFF means the accumulated time has exceeded about 1,000 hours and recommends the replacement of the lamp. * Consult with a Sanyo representative or agent at the time of replacement of UV lamp. Optional parts; UV lamp kit (MCO-20UV) Basic operation sequence (Example: Description of operation 1 Press CAL key for 5 seconds. 2 By pressing key and the figure to F01 3 Press ENT key. 4 By pressing key and the figure to 003. 5 Press ENT key. change of UV lamp ON period from 5 minutes to 3 minutes) Key operated CAL key, set ENT Indication after operation The left digit in the temperature indicator is flashed. When pressed, the changeable digit is shifted. When pressed, the figure of settable digit increases. The current setting is displayed in the CO2 density indicator. When pressed, the changeable digit is shifted. key, set When pressed, the figure of settable digit increases. ENT Set value is memorized and the display return to normal display mode. Note: The available set range for the UV lamp is between 0 and 30 (000 to 030). When set to 000, the UV lamp is not turned on. Condensation will occur and/or temperature distribution may be affected due to the heat of the UV lamp when the setting of the lamp operation is longer than 5 minutes or if the outer door is opened repeatedly. 17 OPERATING INSTRUCTIONS Key lock function This unit is provided with a key lock function. When the key lock is ON, change of temperature or CO2 density setting through the key pad is not available. Note: The key lock is set in OFF mode at the factory. Display Mode Function Key lock is OFF Enable to change of temperature setting Key lock is ON Disable to change of temperature setting Procedure for key lock setting (change from key lock OFF to key lock ON) Description of operation 1 Key operated ---- 2 Press 3 Press to 1. 4 Press ENT key. Indication after operation The current chamber temperature is displayed. LO is displayed in the temperature indicator. key for 5 seconds. key and scroll the figure When pressed, the figure of settable digit increases. ENT The key lock is set to ON. The current chamber temperature is displayed. Note: The key lock function is available for temperature and CO2 density setting. To cancel the key lock, set to L0 in the above procedure. 18 OPERATING INSTRUCTIONS Water level sensor This unit is provided with a water level sensor for the humidifying pan. The sensor is set in active position with respect to the installation of the humidifying pan. Take care not to damage the sensor at the time of removal or installation of the humidifying pan. When the humidifying pan is removed (side view) Sensor Pan When the humidifying pan is installed (side view) Sensor Pan Note: At the installation, check the humidifying pan is located far depth and the sensor is down. Note: * Lift the sensor before installing the humidifying pan if the sensor is in lower position after maintenance. * Use an alcohol for sterilization when cleaning the sensor, taking care not to stress the lead wire. * The sensor detects the water level every 30 minutes. In addition, detection is executed after operation of the outer door. It takes several seconds to detect the water level. Therefore, the water level alarm lamp may flash a few times after the outer door is closed with the humidifying pan filled sufficiently. 19 ALARMS & SAFETY FUNCTIONS This unit has the alarms and safety functions shown in table below, and also self diagnostic functions. Alarms and safety functions Alarm & Safety Upper limit temperature alarm Automatic set temperature alarm Automatic set CO2 density alarm Auto-return Key lock Automatic calibration function Situation If the chamber temperature exceeds the upper limit alarm temperature set value. If the chamber temperature deviates o from the set temperature by ±1 C or more. If the chamber CO2 density deviates from the set value by ±1% or more. When there is no key pressing in each setting mode for 90 seconds. When the key lock is “ON”. Normally, the zero point of the CO2 sensor is calibrated every 4 hours (or very 10 minutes for the first hour after switch ON), using the atmosphere as the gas to be calibrated. Indication Buzzer Over heat lamp lights. E12, E13, E14and chamber Continuous tone temperature are displayed alternately. Heater OFF Remote alarm All digits on the temperature indicator blink. Intermittent tone with 15 minutes delay. Remote alarm with 15 minutes delay All digits on the CO2 density indicator blink. Intermittent tone with 15 minutes delay. Normal display mode. --------- Remote alarm with 15 minutes delay The setting mode is canceled. The setting is disabled. ----- ----- ----The decimal point (period) on the CO2 density indicator blinks. E01 is displayed alternately with the temperature on the temperature indicator. E01 is displayed alternately with the temperature on the When the gas supply line is switched. Gas line switching temperature indicator. (only when MCO-21GC is installed) Gas supply line indicator blinks. E05 is displayed alternately If the temperature sensor is with the temperature on the Chamber disconnected. temperature indicator. temperature sensor E06 is displayed alternately abnormality If the temperature sensor is short with the temperature on the circuited. temperature indicator. If the sensor box temperature sensor is E07 is displayed alternately with the temperature on the Sensor box disconnected. temperature indicator. temperature sensor abnormality If the sensor box temperature sensor is E08 is displayed alternately with the temperature on the short circuited. temperature indicator. E09 is displayed alternately If the ambient temperature sensor is with the temperature on the Ambient disconnected. temperature indicator. temperature sensor E10 is displayed alternately abnormality If the ambient temperature sensor is with the temperature on the short circuited. temperature indicator. CO2 sensor If the output voltage of the CO2 sensor E11 is displayed alternately with the temperature on the abnormality is abnormal. temperature indicator. If the upper limit alarm temperature E12 is displayed alternately Main heater alarm operates, or if the main heater is with the temperature on the abnormality open circuit, or the main heater relay temperature indicator. short circuit. Bottom heater If the bottom heater goes open circuit, E13 is displayed alternately abnormality or the bottom heater relay short circuit. with the temperature on the temperature indicator. E14 is displayed alternately Door heater If the door heater goes open circuit, with the temperature on the abnormality or the door heater relay short circuit. temperature indicator. If the sensor box heater goes open E15 is displayed alternately Sensor box heater circuit, or the sensor box relay short with the temperature on the abnormality circuit. temperature indicator. Disconnection of If the relay of main heater, bottom E16 is displayed alternately sensor for each heater or sensor box heater goes open with the temperature on the heater circuit. temperature indicator. Air pump (for If the air pump (sampling or auto zero) E17 is displayed alternately sample air or does not operate, or if there is with the temperature on the auto-zero) something wrong in the gas piping. temperature indicator. failure Low humidifying If the water in the pan is about 1 liter. RH PAN lamp blinks. water Recommendation of The accumulated ON time is over UV indicator blinks when about new UV lamp UV lamp is OFF. 1,000. CO2 gas cylinder empty Safety operation If the CO2 density does not increase when the gas valve is opened. Intermittent tone Remote alarm Intermittent tone Gas supply line is altered. Remote alarm Intermittent tone Heater OFF Remote alarm Intermittent tone CO2 valve close. Remote alarm Intermittent tone Remote alarm Intermittent tone CO2 valve close. Remote alarm Intermittent tone Remote alarm Intermittent tone Remote alarm Intermittent tone Remote alarm Intermittent tone Remote alarm Intermittent tone Remote alarm Intermittent tone CO2 valve close. Remote alarm ----- ----- ----- ----- * The remote alarm is silenced by pressing the buzzer stop key (BUZZER) as the remote alarm is operated in conjunction with the buzzer. * The upper limit temperature alarm cannot be silenced with the BUZZER key. * E01 is cleared automatically when the gas is connected correctly and the buzzer is silenced with the BUZZER key. If one of E05 to E17 (except for E12, E13, and E14) is displayed, consult with a Sanyo sales representative or agent. 20 ALARMS & SAFETY FUNCTIONS Remote alarm terminal The remote alarm terminal and remote recorder terminals are located at the rear right side. Remote alarm terminal The remote alarm terminal is a contact output. Normal : OPEN Abnormal : CLOSE Contact capacity : DC 30V, 2A, no voltage contact When the power switch is OFF or the power failure condition, the contact output is CLOSE. REMOTE ALARM MAX DC30V 2A N. O. Remote alarm terminal 21 ROUTINE MAINTENANCE WARNING Always disconnect the power supply to the unit prior to any repair or maintenance of the unit in order to prevent electric shock or injury. Ensure you do not inhale or consume medication or aerosols from around the unit at the time of maintenance. These may be harmful to your health. CAUTION Always put on dry gloves to protect hands at the time of maintenance. Failure to use gloves may result in cuts or abrasions from any sharp edges or corners. Sterilizing of chamber and attachments When the chamber of the unit is contaminated, the chamber and internal attachments should be cleaned and sterilized as follows. Note: Take care not to damage the UV lamp or water level sensor at the time of removal or replacement of attachments. Do not clean the inside of the unit with a solution of disodium chlorate or other halogen-based solution because this may cause rust. 1. Take out all trays and the humidifying pan from the chamber. 2. Remove top panel, side support (right and left side), tray supports, and the fan as shown in the figures on page 23 and 24. 3. Clean all the attachments with neutral detergent and then rinse away the detergent with distilled water. 4. Wipe the attachments with a gauze containing alcohol for sterilization and then wipe off with a dry gauze. 5. Wipe the inside wall of the with a gauze containing alcohol for sterilization and then wipe off with a dry gauze. 6. Replace all attachments in the chamber. Note: Always insert the fan on the motor shaft surely. Improper insertion may cause poor performance. 7. Fill the humidifying pan with sterile distilled water. 22 ROUTINE MAINTENANCE Removal of attachments Note: Take care not to damage the UV lamp or water level sensor at the time of removal or replacement of attachments. 1. Close the valve of the gas cylinder and turn off the power. 2. Open the outer door. Remove 2 pins fixing the inner hinge and remove the inner door. 3. Pull out the all trays. See Fig. 1 4. Remove all tray supports on the right and left side by lifting up the front side. See Fig. 2. 5. By lifting up the humidifying pan cover, unhook it at the rear and remove the cover as shown in Fig. 3. 6. Take out the humidifying pan. See Fig. 4 Fig. 1 Fig. 2 Fig. 3 Fig. 4 23 ROUTINE MAINTENANCE 7. After removing 2 screws fixing the top duct, take out the top duct by removing pin. See Fig. 5. 8. To remove the rear duct, lift it up as shown in Fig. 6. 9. To removing the circulating fan, pull out the spring and then pull the fan. See Fig. 7. 10. Loosen and remove the screw fixing the clamp and remove the clamp. See Fig. 8 and Fig. 9. 11. By lifting the side support, take it off from the hooks. See Fig. 10. Fig. 5 Fig. 6 Fig. 7 Fig. 8 Fig. 9 Fig. 10 12. Replace all attachments in the chamber with the reversed order mentioned above. Note: Always insert the fan on the motor shaft surely. Improper insertion may cause poor performance. 24 ROUTINE MAINTENANCE Filling the humidifying pan Replace the water in the humidifying pan when the water level lamp lights. To fill the humidifying pan, do the following: 1. Lift the front side of the humidifying pan cover as shown in Fig. 1. 2. Pull out the humidifying pan toward you. See Fig. 2. 3. Dispose of the water in the pan and wash it with neutral detergent. Then rinse the pan with distilled water sufficiently. Finally, wipe the pan with a soft cloth and alcohol for disinfection. 4. Wipe off the bottom of the chamber completely. 5. Place the pan under the pan cover and pour the sterile distilled water (about 5 liter) into it. See Fig. 3. Make sure that the water is pre-heated at 37oC. 6. Push the pan in to its correct position and replace the cover over the pan. 7. Check that the water level lamp on the control panel is off. Note: The sterile water filled in the humidifying pan should be pre-heated at 37oC. Cold water lowers the chamber temperature. Fig. 1 Fig. 2 Fig. 3 25 CALIBRATION Temperature calibration 1. Press the CAL key for approximately 5 seconds. 2. The third digit of the temperature indicator flashes, and the CO2 density indicator goes out. 3. Set the present correct temperature with the key and key, then press the ENT key. 4. The unit automatically reverts to the display mode. [Example] If the displayed chamber temperature is 37.0oC (set value) and the actual temperature is 36.8oC. 1. Press the CAL key for about 5 seconds. 2. The “3” on the temperature indicator flashes, and the CO2 density indicator goes out. 3. Adjust the set value to the actual value of 36.8oC with the key and key, then press ENT key. 4. The unit automatically reverts to the display mode. Note: It is important to accurately measure the temperature inside the unit when performing temperature calibration. Particularly, the temperature gauge used must have an accuracy of 0.5 Class or better. The temperature must be measured at several points. The temperature setting must not change by more than ±1.0oC during calibration. If it exceeds this, an error tone is emitted, the input data is ignored, and the unit reverts to the display mode. Consequently, if it is necessary to change the temperature by more than 1.0oC, perform calibration in several stages over a period of time. CO2 calibration Span setting Span setting should be done under stable condition of temperature, humidity, and CO2 density. 1. Press the CAL key for about 5 seconds. 2. The third digit on the temperature indicator flashes, and the CO2 density indicator goes out. 3. Press the CAL key once again. 4. The third digit on the CO2 density indicator flashes, and the temperature indicator goes out. 5. Set the present correct CO2 density with the key and key, then press the ENT key. 6. The unit automatically reverts to the display mode. Note: This calibration is available when the setting of CO2 density is 2% or more. [Example] For an internal CO2 density of 5.0% (setting) and a measured value of 4.5%. 1. Press the CAL key for about 5 seconds. 2. The third digit on the temperature indicator flashes, and the CO2 density indicator goes out. 3. Press the CAL key once again. 4. The third digit on the CO2 density indicator flashes, and the temperature indicator goes out. 5. Set the present correct CO2 density (4.5%) with the key and key, then press the ENT key. 6. The unit automatically reverts to the display mode. 26 TROUBLESHOOTING If the unit malfunctions, check out the following before calling for service. The unit does not operate at all 1. The unit is not plugged correctly into a power outlet. 2. The circuit breaker at the power source is active. 3. A power failure has occurred? The key operation is disabled 1. The key lock function is set in OFF mode. If the alarm function operates If the alarm function and the buzzer operates, check the cause using the following procedure. [At the beginning of operation] 1. The chamber temperature is not equal to the set value. 2. The chamber CO2 density is not equal to the set value. a. The secondary pressure of the pressure regulator is not equal to the set value (0.03MpaG, 0.3kgf/cm2G, 4.3psiG). b. The tube is not connected securely between the pressure regulator and the unit. [During operation] 1. The upper limit alarm temperature is not set at least 1oC higher than the set chamber temperature. 2. The set temperature value was changed, or the door was left open for a long period. A low temperature load was placed inside the unit. In this case, if the unit is left as it is, the alarm will eventually clear itself. 3. The gas tube has slipped off. 4. The set value of the gas density was changed. 5. The gas cylinder is empty. Check the primary pressure of the CO2 cylinder once a week. If the chamber temperature is not equal to the set temperature 1. The temperature in the vicinity is too high. The ambient temperature must always be at least 5oC less than the set temperature. 2. The outer door was closed while the inner door was left open. If the gas density does not coincide with the set value 1. The secondary pressure is not set to 0.03MPaG (0.3kgf/cm2G, 4.3psiG). 2. The gas tube is clogged or chinked. If the chamber humidity does not rise 1. The humidifying pan is not filled with sterile distilled water. (Always use sterile distilled water.) 2. The humidifying pan cover is not installed correctly. If the CO2 consumption is too much 1. The door is opened frequently. 2. There is any gas leakage at the connection or pin hole on the tube. It is recommended to replace the tube once a year. 3. The gasket of the inner door is not completely sealed. 4. The access port at the upper left corner is opened. If normal cultivation cannot be done and chamber gas density is suspect 1. The environment around the unit is not normal. The source of the contaminated gas is nearby. 2. The unit is installed in an enclosed space. If it takes much time to recover the gas density 1. HEPA filter is provided in the gas piping. If it takes much time to recover the gas density even though the gas pressure is normal, it may be that dust on the HEPA filter prevents the gas flow. Consult the Sanyo dealer or agent. 27 ENVIRONMENTAL CONDITIONS This equipment is designed to be safe under the following conditions (based on the IEC 1010-1): 1. Indoor use; 2. Altitude up to 2000 m; 3. Ambient temperature 5oC to 35oC 4. Maximum relative humidity 80% for temperature up to 31oC decreasing linearly to 50% relative humidity at 40oC; 5. Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage; 6. Other supply voltage fluctuations as stated by the manufacturer; 7. Transient overvoltages according to Installation Categories (Overvoltage Categories) II; For mains supply the minimum and normal category is II; 8. Pollution degree 2 in accordance with IEC 664. DISPOSAL OF UNIT WARNING If the unit is to be stored unused in an unsupervised area for an extended period ensure that children do not have access and doors cannot be closed completely. The disposal of the unit should be undertaken by appropriate personnel. Always remove doors to prevent accidents such as suffocation. 28 STACKED MODULE This unit can be stacked by using the stacking kit. Following shows the procedure for stacking the unit. Consult with a Sanyo representative or agent prior to stacking procedure as such work involves dangers. WARNING Select a level and sturdy floor having enough strength for installation of stacked module. Never stack 3 or more units. Take care not to drop or tip over the unit when stacking as this can cause injury or damage of the unit. 1. Turn off the power switch and disconnect a plug of each unit. 2. The stacking plate A and B are attached on the back of each unit. To prepare the stacking plate A and B, remove the rear cover by unscrewing 6 screws on the rear cover. See the figure below. 3. Take out the stacking plate A and B by loosening 2 fixing screws on each unit. 4. Replace the rear cover and fix it with 6 screws. 5. Check that the lower unit is level. Stacking plate A Stacking plate B Fixing screws Rear cover 29 STACKED MODULE 6. Apply the protective sticker enclosed with the unit at each corner on the top of the lower unit to avoid scratches or damage. 7. Fix the stacking plate A at 2 locations on the top front of the lower unit by using 2 screws removed in step 3. 8. Remove the front panel on the upper unit by unscrewing the 4 fixing screws and then disconnect the wires and gas tube. 9. Stack the unit so that both units can be aligned straight. Also check the upper unit is level. If it is not level, keep the unit even by adjusting the leveling legs. 10. Secure the upper unit with the stacking plate A and 2 screws removed in step 3. 11. Remove 2 hooks on the rear side of the lower unit by unscrewing each 2 fixing screws. 12. Remove 1 screw on the bottom right and left on the rear side of the upper unit. 13. Fix the stacking plate B at the right and left on the rear of the lower and upper unit with 3 screws removed in step 11 and 12. 14. Replace the front panel on the upper unit after connecting the wires and gas tube. 15. Fix the stacked unit to the wall with 2 hooks on the rear of the upper unit and rope or chain. Front panel Stacking plate B Stacking plate A Hook Protective sticker 30 SPECIFICATIONS Name CO2 Incubator Model MCO-20AIC External dimensions W770 x D708 x H900 (mm) Internal dimensions W620 x D523x H665 (mm) Interior volume 215 L Exterior Polyester finish baked on zinc galvanized steel Interior Stainless steel containing copper Outer door PMMA/PVC Inner door Tempered glass Tray Access port Insulation Heating system 5 trays made of stainless steel containing copper W580 x D450 x H12 (mm), Maximum load; 5 kg/tray Inner diameter; 30 mm, On the back side Rigid polyurethane foamed-in place (CFC-FREE) DHA system (heater jacket + air jacket system) Heater Humidifying system 395 W Natural evaporation with humidifying pan Temperature controller PID control Temperature display Digital display CO2 controller ON-OFF control system CO2 density display Digital display Air circulation Fan assisted Air filter 0.3μm, Efficiency; 99.97% UV lamp 4 W, Ozoneless type, Luminescence wavelength; 254mn (GL4) Optical type Water level sensor Alarm Remote alarm contact Temperature alarm, CO2 density alarm, Upper limit temperature alarm, Door alarm Allowable contact capacity: DC 30 V, 2 A CO2 inlet connection 4 to 6 mm diameter tube 0.03MPaG (0.3kgf/cm2G, 4.3psiG) CO2 inlet pressure Accessories Weight Optional accessory Maximum power consumption Total maximum current 5 trays, 5 sets of tray support, 1 gas tube, 1 humidifying pan, Stacking plate A and B (fixed on rear of the unit), 2 tube bands 106 kg CO2 pressure regulator (MCO-100L) Roller base (MCO-20RB) Automatic gas switcher (MCO-21GC) Extra tray (MCO-58ST) including 2 tray supports UV lamp kit (MCO-20UV) Independent inner door (MCO-20ID) Stacking base (MCO-20SB) for stacking MCO-17AIC/17AC/15AC on MCO-20AIC 380 W 110 to 120 V, 60 Hz 3.7 A 220 V, 60 Hz 1.9 A 220 to 240 V, 50 Hz 1.9 A Note: Design or specifications will be subject to change without notice. The ballast is not user replaceable part. Please contact a qualified service personnel. 31 PERFORMANCE Usable environment condition Temperature control range Temperature distribution Temperature variation CO2 control range CO2 variation Chamber humidity Temperature; +5oC to 35oC, Humidity; equal or less than 80% R.H. Ambient temperature +5oC to 50oC (ambient temperature; 5oC to 35oC) ± 0.25oC* (ambient temperature; 25oC, setting; 37oC, 5%, no load) ± 0.1oC (ambient temperature; 25oC, setting; 37oC, 5%, no load) 0 to 20% ± 0.15% (ambient temperature; 25oC, setting; 37oC, 5%, no load) 95 ± 5% R.H. 1370 kJ/h Maximum heat emission 30 dB (A scale) Noise level Note: The unit with CE mark complies with EC directives 89/336/EEC, 93/68/EEC and 73/23/EEC. * It is based on our measuring method. 32 CAUTION Please fill in this form before servicing. Hand over this form to the service engineer to keep for his and your safety. Safety check sheet 1. Incubator contents : Risk of infection: Risk of toxicity: Risk from radioactive sources: □Yes □No □Yes □Yes □Yes □No □No □No (List all potentially hazardous materials that have been stored in this unit.) Notes : 2. Contamination of the unit Unit interior No contamination Decontaminated Contaminated Others: □Yes □No □Yes □Yes □Yes □No □No □No 3. Instructions for safe repair/maintenance of the unit □Yes □No a) The unit is safe to work on □Yes □No b) There is some danger (see below) Procedure to be adhered to in order to reduce safety risk indicated in b) below. Date : Signature : Address, Division : Telephone : Product name : CO2 incubator Model : Serial number : MCO-20AIC Please decontaminate the unit yourself before calling the service engineer. 33 Date of Installation : 7FB6P101283009 (19 April 2004) Recycled paper SANYO Electric Biomedical Co., Ltd. CEM 10-15, Hongo 3-Chome Bunkyoku, Tokyo 113-8434 Japan Printed in Japan ">
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Key features
- Precise temperature and CO2 control
- Built-in UV lamp for sterilization
- Automatic calibration function for the CO2 sensor
- Alarms for various conditions
- Easy to use
Frequently asked questions
The UV lamp is used to sterilize the water in the humidifying pan and the air circulating in the chamber.
Refer to the 'Set of chamber temperature and CO2 density' section on page 16 of the manual.
The 'RH PAN' lamp flashes when the water in the humidifying pan is less than approximately 1 liter.