CUV-UV Cuvette Holder
CUV-UV Cuvette Holder
Installation Instructions
The CUV-UV cuvette holder for 1-cm path length cuvettes couples via SMA-terminated optical fibers
to our spectrometers and light sources to create small-footprint spectrophotometric systems for
absorbance and transmission experiments. This holder can be optimized for UV-VIS-NIR (~200–
2000 nm) applications.
Parts Included
Cuvette holder assembly for holding 1-cm cuvettes
Two barbed fittings for connecting a temperature stabilizing water source
Screwdriver for adjusting the fit of the cuvette
Allen wrench for adjusting the collimating lenses
Attaching the Fibers
► Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses. Attach the
other end of this fiber – the illumination fiber – to a light source.
2. Attach another SMA-terminated optical fiber to the other collimating lens. Attach the other
end of this fiber – the read fiber – to the spectrometer.
CUV-UV Cuvette Holder Installation Instructions
Using the Fiber Supports
► Procedure
1. Snap the clamps around the fibers after the fibers are screwed into the light source and
2. Lift the clamps until they support the fibers.
3. Unsnap the clamps to remove the fibers.
4. Insert the filter into the filter slot. The filter slot can accommodate filters up to 6 mm thick.
5. Clamp the filter in place by gently tightening the clamping screw finger-tight.
Adjusting the Fit of the Cuvette
The Cuvette Holder is designed to hold 1-cm square cuvettes. When properly adjusted, the cuvette
should fit snugly into the holder.
► Procedure
1. Locate the two ball plunger screws.
2. Remove the fiber clamps for easier access to the screws.
3. Use the provided screwdriver to loosen the two ball plunger screws until the ball end of the
screws is just visible in the cell holder and insert your cuvette into the holder.
4. Tighten the ball plunger screws until the ball contacts the cuvette and starts to compress. Do
not over-tighten.
Using the Temperature Stabilization Feature
This feature is used to heat or cool the cuvette holder base and cuvette.
► Procedure
1. Remove the plugs from the top side of the base. (The plug on the right side of the base should
stay in place but may require thread tape.)
2. Replace the plugs with the two barbed fittings (or any 1/8" NPT pipe thread fittings). Thread
tape may be required on the fittings.
3. Connect the fittings to a water source. Water will circulate through the base.
CUV-UV Cuvette Holder Installation Instructions
58 mm x 140 mm x 38 mm
230 kg
Path length
1 cm
Z dimension
15 mm
Collimating lenses (VIS-NIR)
BK 7 glass (~360 nm – 2 m*), 5 mm diameter, f/2
Collimating lenses (UV-VIS-NIR)
Quartz (200 nm-1.3 m), 5 mm diameter, f/2
Collimating lens termination
SMA 905
Collimating lenses assembly (sample compartment)
2.0" x 1.5" (LW)
Filter slot
accepts filters up to ¼" (6 mm) in thickness
Base material
Connections in base
800 (deuterium); 2,000 (tungsten)
Water input fittings
water inlet, water outlet, extra connection;
3.175 mm (1/8”) NPT
Typical optical fibers specified for optimum performance
(light throughput and optical resolution)**
200 m illumination fiber, 50 m read fiber
Though the VIS-NIR lens is optimized for use to 2 m, it can be configured to "see" only to 1100 nm with
our S2000 spectrometer.
For all intents, there is no single combination of optical fibers that will satisfy the requirements of every
application. As a rule, however, it is best to use a large-diameter (>50 m) illumination fiber to get the
maximum light throughput, and a small-diameter (<50 m) read fiber to achieve the best optical
CUV-UV Cuvette Holder Installation Instructions
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