Biometra Thermal Cyclers 2012-2013
TH ER MOCYCLER
Thermocycler
Introduction ........................................................................................................................... 16
TOptical Thermocycler ......................................................................................................... 17­
TProfessional Family ............................................................................................................ 22
TProfessional Thermocycler .............................................................................................. 24
TProfessional Standard Thermocycler ............................................................................ 25
TProfessional Basic Thermocycler ................................................................................... 26
TProfessional TRIO Thermocycler .................................................................................... 27
TProfessional Manager Software ..................................................................................... 29
TPersonal Thermocycler ..................................................................................................... 30
TRobot Thermocycler .......................................................................................................... 31
Technical Specifications ...................................................................................................... 32
Thermocycler Capacity and Suitable Consumables ................................................... 34
Order Information ................................................................................................................ 35
Oligonucleotides ................................................................................................................... 37
Innovation · Advancement · Development
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Thermocycler
Introduction
Biometra designs and manufactures
thermocyclers for more than 20 years
for all needs and all fields of application.
No matter what tubes or plastic ware
you are using, what sample volume or
throughput you need, Biometra offers
the right instrument.
High precision instruments
All Biometra Thermocyclers are high
precision instruments. This becomes
evident by the excellent temperature
homogeneity provided by all Biometra
sample blocks. To achieve highest
heating and cooling rates, different
technologies are used for the different
thermocycler models.
High Performance Smart Lid (HPSL)
All Biometra Thermocyclers feature a
special lid mechanics ensuring optimum
pressure for all kind of tubes. This defined
pressure is not only important to prevent
condensation at the tube walls but also
to ensure a tight contact between the
tube walls and the sample block.
Only this tight contact guarantees an
efficient heat transfer, thus enabling
fast temperature equilibration of the
reaction liquid. In addition, all Biometra
instruments feature an extended lid
frame that provides for a homogeneous
temperature cushion above the block
surface. Together with the high precision
sample blocks, an excellent temperature
homogeneity is achieved between all
samples.
Easy Spreadsheet Programming (ESP)
All Biometra Thermocyclers provide
spreadsheet programming. Avoiding
endless serial program windows, all
steps of a program are entered in a
single screen. This makes the creation
of new or editing existing programs
fast and easy. The navigation with
four cursor keys provides maximum
convenience.
Low noise and heat emission
By using efficient Peltier technology
and an intelligent power management,
Biometra Thermocyclers are optimized
for minimum power consumption.
Due to the low energy uptake the
thermocycler heat sink does not require
extensive ventilation during the heating
and cooling phases. This is the basis
for the low heat emission and silent
operation of Biometra Thermocyclers.
Flexibility
Biometra Thermocyclers are as flexible
as our customers needs. To support
the usage of different types of plastic
ware, we offer a broad range of
exchangeable block modules for the
TProfessional Thermocycler. The block
modules can be exchanged within a
few seconds. For the optimisation of
16
Innovation · Advancement · Development
new primer pairs they are also available
with gradient function. Additionally,
the TProfessional base unit can also
be upgraded for Real-Time PCR by the
TOptical block module.
The TProfessional TRIO is equipped
with three blocks that allow to run three
different protocols at the same time.
TH ER MOCYCLER
TOptical Thermocycler
The Real-Time PCR System with Maximum Flexibility
• Free configurable with up to 6 color
filter modules for excitation and
detection of commonly used
fluorescent dyes
• Up to 6 fold multiplexing
• Excitation by three different
coloured long living LEDs
• Patented array of high performance
optical fibers for loss-free excitation
and detection of fluorescence
• Highly sensitive Channel Photo
Multiplier (CPM) with efficient
noise reduction
• TProfessional Thermocycler
retrofittable with optical module
• Block with or without gradient
function
• Excellent heating and cooling rates
and temperature uniformity
• Easy to use software for creating
experiments, for analysis and data
export
Modular block concept
The TOptical Thermocycler consists
of the base unit of the TProfessional
Thermocycler and the TOptical module.
This modular system enables the
upgrade of existing TProfessional
Thermocyclers by the TOptical module to
a complete real-time PCR Thermocycler.
Simply insert the TOptical module and
that’s it – in a few seconds you can
begin with real-time PCR. Or the
TOptical module can be quickly
exchanged for a TProfessional module
and the TOptical Thermocycler be used
as conventional thermocycler. For maximum flexibility five different modules
in various block formats, ranging from
0.5 ml tubes up to 384 well microplates,
are available.
Innovation · Advancement · Development
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Optional gradient function
Finding the best annealing temperature is, especially for real-time PCR
experiments very important. Often
time-consuming optimisation tests are
conducted only limited, which leads to
suboptimal results under routine
conditions. To allow the testing of new
primer combinations in a single run,
the TOptical module optionally is
available with gradient function.
With a gradient span of 40 °C and the
linear gradient tool for programming of
equal temperature increments between
the 12 columns of the block the
TOptical fulfills every wish for easy
optimisation of real-time PCR assays.
New primer pairs with unknown
annealing temperatures can be tested
quickly and new protocols are optimized in a very short time.
Flexible configuration
with optical filter modules
The patented* optical system of the
TOptical Thermocycler provides a filter
wheel for 6 color filter modules. The
filter wheel can be freely equipped with
color filter modules of choice. Furthermore, the system can also subsequently
be upgraded with color filter modules
and so the range of applications
extended. In total 10 different color
filter modules are available, covering
the whole range of commonly used
real-time PCR dyes, from the blue to
the red absorption spectrum, including
filter modules specially optimized for
FRET applications.
* Patent No. DE 2006 036 171 B4
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Innovation · Advancement · Development
TH ER MOCYCLER
LED technology
The fluorescent dyes are excited by
three LEDs in the colors blue, white and
red. The combination of these three
LEDs allows optimal excitation of
fluorescent dyes over a wide spectral
range. Unlike with so-called „wide blue“
LEDs especially the short-wavelength
blue spectral range and the long-wavelength red range are optimally covered.
In combination with the high-performance optical fibers and the highly-
TOptical Lightpath
Schematic drawing of the patented optical sytem.
Fluorescent light for the excitation of dyes is emitted
by the LEDs. The light is transmitted by high
performance optical fibers to collimator lenses.
The fluorescent light is bundled and transmitted to
an excitation filter of a color filter module located
on a rotating filter wheel. The light is deflected by
a beam splitter and transferred by optical fibers
to a lens array in a shuttle that scans the sample
block column by column. The light excites the
fluorescent dyes in the reaction mix. The fluorescent dyes then emit light of a higher wavelength
that is collected by the lenses in the shuttle system
and transferred by optical fibers back to the filter
color module. In the color module the light passes
the beam splitter and two emission filters and is
then further transferred to the channel photo
multiplier for detection.
sensitive Channel Photo Multiplier
intensity losses in the quantum efficiency
are avoided to ensure maximum
sensitivity. Due to the excitation of
each single well the use of passive
reference dyes is not necessary, allowing multi-plexing of up to six targets.
The longevity of the LEDs makes a
regular exchange of the light source
superfluous and thus helps
avoid recurring costs.
Emission filter (interference)
Emission filter (glas)
CPM Detector
Beam splitter
Ferrule
Optical fiber
LED light source
Excitation filter
Lens
Collimator lens
Ferrule
Optical fiber
Lens array in shuttle system
Sample block
Optimal excitation of fluorescent dyes
FAM
100
LED Emission/Absorption
TOptical Excitation by LED
The excitation spetra of the blue, white and red
TOptical LED (dotted lines) cover very well the absorption spectra of the commonly used fluorescent
dyes FAM, JOE/HEX/VIC, 6-ROX, TET, TAMRA, CY5
and Cy 5.5 (solid lines). In combination with the
beam transfer by high perfomance optical fibers
superb sensitivity is achieved.
90
JOE/HEX/VIC
80
6-ROX
70
TET
60
TAMRA
50
Cy5
40
Cy5.5
30
LED blue
20
LED white
10
LED red
0
400
450500550600650
700 nm
Innovation · Advancement · Development
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Software
The TOptical Thermocycler is controlled
by PC software qPCRsoft. The software
is especially developed to provide
ease of use and clear arrangement of
functional elements.
It includes the same easy spreadsheet
or graphical programming function as the
TProfessional Thermocycler. Information
on sample is entered in a well arranged
plate scheme that provides a
comprehensive overview at a glance.
Typical evaluation methods of the
real-time PCR such as ∆∆Ct method,
absolute quantification, relative
quantification, allelic discrimination,
genotyping and efficiency calculation
are already integrated. In addition, the
software features the export of data
as *.csv format or Excel files for further
external analysis.
Practice of patented polymerase chain reaction
(PCR) process requires a license. Please see
Appendix, page 181.
Order Information
Item
Properties
Dyes
Order No.
TOptical Gradient 96 Thermocycler
without color filter module
—
070 - 500
TOptical 96 Thermocycler
without color filter module
—
070 - 501
TOptical Gradient 96 module
without color filter module
—
070 - 510
TOptical 96 module
without color filter module
—
070 - 511
TOptical filter module 1
470 nm / 520 nm
FAM, SYBR Green, Alexa488
070 - 520
TOptical filter module 2
515 nm / 545 nm
JOE, VIC, HEX, Yakima Yellow
070 - 521
TOptical filter module 3
535 nm / 580 nm
TAMRA, DFO, Alexa 546, NED
070 - 522
TOptical filter module 4
565 nm / 605 nm
ROX, TexasRed, Cy3.5
070 - 523
TOptical filter module 5
630 nm / 670 nm
Cy5, Alexa 633, Quasar 670
070 - 524
TOptical filter module 6
660 nm / 705 nm
LightCycler Red 705, Alexa 680
070 - 525
TOptical FRET filter module 1
470 nm / 580 nm
FAM / TAMRA
070 - 526
TOptical FRET filter module 2
470 nm / 670 nm
FAM / Cy5
070 - 527
TOptical FRET filter module 3
470 nm / 705 nm
FAM / Cy5.5
070 - 528
TOptical FRET filter module 4
515 nm / 670 nm
JOE / Cy5
070 - 529
Additional filter combinations available on request
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Innovation · Advancement · Development
TH ER MOCYCLER
TOptical Thermocycler
Technical Specifications
Block format
96 well
Lid temperature
30 – 99 °C
Temperature gradient
40 °C
Max. heating rate
6.0 °C / sec
Avg. heating rate
5.0 °C / sec
Max. cooling rate
4.5 °C / sec
Avg. cooling rate
3.5 °C / sec
Ramp adjustment
min 0.1 °C / sec, max. 5.0 °C / sec
Block temperature uniformity
(15 sec after clock starts)
± 0.15 °C at 55 °C
± 0.25 °C at 72 °C
± 0.50 °C at 95 °C
Control accuracy
± 0.10 °C
Temperature range
3 – 99 °C
Temperature increments
min. 0.1 °C / cycle
Time increments
min. 1 sec / cycle
Heated Lid
manual opening mechanism, automatic pressure application
Contact pressure heated lid
10 kg, automated
Control mode
remote controlled via PC
No. of programs
unlimited on PC
Dimensions
28 cm x 38 cm x 43 cm, 28 cm x 64 cm x 43 cm when opened
Weight
15.6 kg (TOptical module 7.4 kg, TProfessional base unit 8.2 kg)
Power supply
100 V, 110 V, 230 V
Operating conditions
15 °C to 35 °C, max. 70 % humidity, max. 2000 m height
Supported plastic ware
96 well microplates with adhesive optical foil, strips of 8, 0.2 ml with optical lids,
0.2 ml single tubes with optical lids
Sensitivity
1nmol / l FAM at 30 µl sample volume in a 96 well PCR plate
Measuring time
96 well plate (single measurement, 6 colors) appr. 6 sec
Measuring range
± 130 000 bit (±17 bit)
Block capacity
96 well
Sample volume
10 – 80 µl
Light source
Three long living high power LEDs (blue, white, red)
Filter
Filterwheel with stepping motor, 6 positions for color modules
Lightpath
An array of 8 high performance optical fibers in a shuttle system directs LED light bundled by lenses to
samples. The fluorescent dyes in the reaction mix are excited from above through the lid of the tubes.
The reflected light is focused by lenses and directed through optical fibers to the photomultiplier
Detector
Highly sensitive channel photo multiplier (CPM), Optimal signal/noise ration by
effective noise reduction (decreased SNR (signal/noise ratio)-technology)
Color modules
6 color modules for all commonly used Real-Time PCR dyes, 4 FRET filter combinations
Software
qPCRsoft
Control and analysis software
Analysis methods
Absolute quantification, Relative quantification, ∆∆Ct method, Allelic discrimination, Genotyping,
Efficiency calculation
Export functions
Excel, *.csv files
Security
Administrator function
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TProfessional Family
The Biometra TProfessional Thermocycler
Family is inspired by the idea to simplify
molecular biology research. Driven by
Biometra‘s 20-year experience in
Thermocycler design and production
the TProfessional Thermocyclers were
developed to set new a unrivalled
standard. The instruments combine
high end technology with functional
and elegant design. Due to the energy
efficient design of the housing, lid and
block very high speed and excellent
temperature uniformity are realised.
Maximum comfort is provided to the
user by the large display and innovative
operating software. The result is four
easy to use instruments with excellent
technical specifications and highest
ease of use:
High Performance Smart Lid
The TProfessional Lid has been optimised to achieve two essential requirements: prevention of condensation and
reliable contact between samples and
thermoblock. The lid is powered by
Biometra‘s well known Smart Lid
technology with integrated clutch
mechanism which automatically limits
the lid pressure applied to the plastic
ware and thus saves tubes from damage.
This also ensures reproducible conditions
amongst different PCR runs. In addition,
due to the new design of the High Performance Smart Lid, by formation
of a homogeneous air cushion an
even temperature distribution
between the samples is
ensured, significantly
improving temperature uniformity.
With one press on the front button, the
lid gently swings open and arrests in its
end position. Moreover the robustness
of the lid‘s mounting serves for a long
lifetime.
1. TProfessional Basic for routine
applications
2. TProfessional Standard with high
speed silver block for advanced
demands
3. TProfessional with high speed silver
block and block exchange system for
maximum flexibility
4. TProfessional TRIO with three
independent blocks to run different
PCR protocols in parallel or for high
throughput purposes.
Air stream design
The elegant TProfessional housing
is designed for heavy use. Due to an
optimised air stream design even higher
efficiency and better temperature
uniformity is achieved. Therefore the
instrument works quietly and consumes
little power which in turn leads to low
heat emission. The compact footprint
saves valuable bench space. Keypad
and display have been set to an angle
that ensures reflection-free viewing and
ergonomic programming.
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Innovation · Advancement · Development
TH ER MOCYCLER
Easy spreadsheet or
graphical programming
The TProfessional user interface
incorporates Biometra‘s proven easy
spreadsheet programming philosophy.
All steps of a program are entered in
a single screen avoiding endless serial
programming windows. This makes
the creation of new or editing of existing
programs fast and easy. One touch
leads from the spreadsheet to the
alternative graphical programming
mode. Programming of gradients has
never been easier due to the linear
gradient tool. The TProfessional manages
up to 30 individual users (optionally
password protected) and features larger
memory for increased program capacity.
Supervisor functions provide easy
administration of user directories.
Quickstart of the last five programs
The TProfessional displays the five most
recently used programs for quick start.
Thus, commonly used programs can
be started without searching the user
directory. The last programs are saved
for each user individually.
Pre-installed protocols
All Thermocyclers of the TProfessional
family have a folder with pre-installed
protocols for various PCR applications.
The protocols can be copied into any
user directory and modified specifically.
The pre-installed protocols therefore
offer an excellent tool to obtain the
desired result as quickly as possible and
without much programming effort.
Convenient setup of temperature
gradients* or temperature
optimisations steps**
Gradient steps are defined in a graphical
screen, showing the individual
temperature for each row. Another
useful feature is the Linear Gradient
Screen, where linear gradients with
fixed temperature increments between
the single rows can be programmed.
Alternatively, the temperature gradient
can be defined directly in the programming spreadsheet.
For the TProfessional TRIO Thermocycler
by the new Temperature Optimisation
function gradient-like programs, delivering
three different annealing temperatures
at a certain step can be created easily.
Programming
Quickstart
* only for gradient enabled instruments
**only für TProfessional TRIO
Network capability
TProfessional Manager Software allows
to control up to 5 Thermocyclers of the
TProfessional family*** in a powerful
network. PCR runs can be managed
and monitored and versatile memory
management functions allow easy
synchronisation of the connected
TProfessional Thermocyclers.
Detailed run log files provide GLP
conform documentation of PCR runs.
See also page 29.
Gradient
*** except TProfessional TRIO
Temperature optimisation step
Innovation · Advancement · Development
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TProfessional Thermocycler
Premium Performance with Maximum Flexibility
•
•
•
•
•
High speed silver block
Quick block exchange
Seven different block modules
Excellent temperature uniformity
Upgradeable for Real-Time PCR
High speed silver block
To achieve ultimate performance the
TProfessional 96 well and 60 well
modules are made of silver. Thanks
to the excellent heat conductivity of
silver, the block equilibrates extremely
fast and provides maximum speed and
temperature uniformity. To protect the
valuable silver blocks against corrosion,
the block surface is covered with a gold
layer.
Exchangeable block modules
The TProfessional offers seven different
block modules that can be exchanged
in less than 10 seconds. The quick
exchange block modules are
automatically recognized and initialised
by the thermocycler. The 96 well and
60 well modules are available with
gradient option for quick optimisation
of new protocols. With an extremely
wide gradient span of 40 °C the blocks
can be used for a variety of other
applications such as e.g. restriction
digests or protein crystallization.
For high throughput applications a
384 well block is available.
Real-Time PCR
By the TOptical module the TProfessional
can be upgraded to a Real-Time PCR
thermocycler. The block exchange is
as easy as with standard PCR block
modules and can be carried out within
a few seconds. The TOptical module is
optionally available with or without a
gradient function. See also page 17.
TProfessional
Order number
Item
Block format
Suitable plastic ware
070 - 900
TProfessional 60
60 well
0.5 ml tubes
070 - 901
TProfessional 96
96 well
0.2 ml tubes, plates, strips
070 - 902
TProfessional 384
384 well
384 well plates
070 - 800
TProfessional Gradient 60
60 well
0.5 ml tubes
070 - 801
TProfessional Gradient 96
96 well
0.2 ml tubes, plates, strips
TProfessional Thermocycler block modules
Order number
Block format
Suitable plastic ware
Max. heating and
cooling rate
070 - 810
60 well Gradient
0.5 ml tubes
6.0 / 4.5 °C per sec
070 - 910
60 well
0.5 ml tubes
6.0 / 4.5 °C per sec
070 - 811
96 well Gradient
0.2 ml tubes, plates, strips
6.0 / 4.5 °C per sec
070 - 911
96 well
0.2 ml tubes, plates, strips
6.0 / 4.5 °C per sec
070 - 912
384 well
384 well plates
2.2 / 1.7 °C per sec
TProfessional Thermocycler Real-Time PCR block modules
Order number Block format
Suitable plastic ware
Max. heating and
cooling rate
070 - 510
96 well
Gradient TOptical
0.2 ml tubes, plates, strips
6.0 / 4.5 °C per sec
070 - 511
96 well TOptical
0.2 ml tubes, plates, strips
6.0 / 4.5 °C per sec
Technical Specifications
Practice of patented polymerase chain reaction
(PCR) process requires a license. Please see
Appendix, page 181.
24
Innovation · Advancement · Development
see page 30 – 31
Ordering Information
see page 33 – 34
TH ER MOCYCLER
TProfessional Standard Thermocycler
Top Performance for Advanced Demands
• High speed silver block
• Five different block modules
• Excellent temperature uniformity
• Same features as the TProfessional Thermocycler
• Available as SL version for plates
with raised rim
TProfessional Standard SL
The TProfessional Standard SL features
a special heated lid that additionally
allows to incubate plates with raised
rim. Regardless if low-profile 0.1ml
plates, 0.2 ml standard plates, tubes
or stripes all kind of plastic ware can
be used with the heated lid of the
Standard SL.
The TProfessional Standard Thermocycler
offers the same technical features as
the TProfessional Thermocycler, but in
contrast to that has a fixed block that
cannot be exchanged.
High speed silver block
To achieve ultimate performance the
TProfessional Standard 96 well and the
60 well block are made of silver. Thanks
to the excellent heat conductivity of
silver, the block equilibrates extremely
fast and provides maximum speed and
temperature uniformity. Both block
variants are available with gradient
option for quick optimisation of new
protocols. To protect the valuable silver
blocks against corrosion, the block
surface is covered with a gold layer. For
high throughput applications a 384 well
block is available.
TProfessional Standard SL heated lid
TProfessional Standard
Order number Item
Block format
Suitable plastic ware
070 - 950
TProfessional Standard 60
60 well
0.5 ml tubes
070 - 951
TProfessional Standard 96
96 well
0.2 ml tubes, plates, strips
070 - 971
TProfessional Standard SL 96
96 well
0.2 ml tubes, plates (also
with raised rim), strips
070 - 952
TProfessional Standard 384
384 well
384 well plates
070 - 850
TProfessional Standard
Gradient 60
60 well
0.5 ml tubes
070 - 851
TProfessional Standard
Gradient 96
96 well
0.2 ml tubes, plates, strips
070-871
TProfessional Standard Gradient SL 96
96 well
0.2 ml tubes, plates (also
with raised rim), strips
Technical Specifications
Practice of patented polymerase chain reaction
(PCR) process requires a license. Please see
Appendix, page 181.
see page 30 – 31
Ordering Information
see page 33 – 34
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TProfessional Basic Thermocycler
Professional Technology for Routine Applications
• Gold anodised or anodized XL
sample block
• Available with or without gradient function
• High temperature uniformity
• High heating and cooling rates
70µl
Gold anodised 96 well or anodised
96 well XL sample block
The TProfessional Basic makes the
TProfessional technology affordable for
routine applications. The instrument
features a gold anodised 96 well block
for standard volumes up to 70 µl or
an anodised XL block for extra large
volumes up to 100 µl such as sample
preparation for Next Generation
Sequencing or bisulfite conversion for
DNA methylation studies. Both blocks
offer the identical heating and cooling
rates and superior temperature uniformity. The TProfessional Basic provides
full functionality of easy spreadsheet
and graphical programming and user
specific quick start of the last five
programs. Both blocks are optionally
available with gradient function for the
quick optimisation of new primer pairs.
100µl
96 well block
96 well XL block
TProfessional Basic
Order number Item
Block format Suitable plastic ware
070 - 601
TProfessional Basic Gradient
96 well
070 - 602
TProfessional Basic Gradient XL 96 96 well XL
0.2 ml tubes, plates, strips
070 - 701
TProfessional Basic
96 well
0.2 ml tubes, plates, strips
Technical Specifications
070 - 702
TProfessional Basic XL 96
96 well XL
0.2 ml tubes, plates, strips
see page 30 – 31
0.2 ml tubes, plates, strips
Practice of patented polymerase chain reaction (PCR) process requires a license.
Please see Appendix, page 181.
26
Innovation · Advancement · Development
Ordering Information
see page 33 – 34
TH ER MOCYCLER
ProfessionalTRIO
TProfessional TRIO Thermocycler
Thermocycler
Three Thermocyclers in one for Maximum Flexibility
and High Sample Throughput
• Three independent thermocyclers
in one housing
• Runs three different programs
at the same time
• Maximum throughput 144 samples
• Three different block formats
available
• Intuitive easy spreedsheet and
graphical programming
• Versatile USB functions
Three independent Thermocyclers
in one instrument
The TProfessional TRIO Thermocycler
offers three independent blocks in one
housing thus different independent
protocols can be run at the same time.
Moreover by the new temperature
optimisation function gradient-like
programs, delivering three different
annealing temperatures can be created
easily. This makes the TProfessional
TRIO Thermocycler the perfect instrument
for laboratories with the demand for
high flexibility and the need to often
optimise new PCR-protocols. With a
maximum capacity of 3 x 48 wells the
TProfessional TRIO also offers high
throughput in parallel.
The TProfessional TRIO Thermocycler
is available in three different block
versions for 48 x 0.2 ml tubes, 30 x
0.5 ml tubes or as combi block version
for 48 x 0.2 ml or 18* x 0.5 ml tubes.
Easy programming
The TProfessional TRIO Thermocycler
offers the intuitive TProfessional software
with easy spreadsheet programming
philosophy. New programs are easily
created in a well arranged screen that
avoids the need to toggle between
different windows. This makes the
creation of new or editing existing
programs fast and easy. One touch
leads from the spreadsheet to an alternative graphical programming mode.
Four softkeys directly below the display
offer quick access to all functions
needed in the individual context and
four menu buttons above the display
to the main menus. For easy retrieval,
programs can be stored in 30 individual
subdirectories (optionally password
protected). By the administrator
function the accumulation of unused
programs or user directories can be
managed to keep the memory
content up-to date.
Keyboard and display are arranged at
an angle to ensure reflection-free
viewing and ergonomic programming.
High speed
By the elegant housing with improved
air stream design and a completely new
hardware environment the TProfessional
TRIO achieves higher heating and
cooling rates and better temperature
uniformity. High ramping rates provide
both short experimental times and
increased specificity. The instrument
works quietly and consumes little
power, which in turn leads to low heat
emission. The compact footprint saves
valuable bench space.
* capacity increases to 35 x 0.5 ml tubes
by use of small cap tubes
Innovation · Advancement · Development
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Smart lid technology
The TProfessional TRIO Thermocyclers
heated lids prevent condensation
and provide reliable contact between
samples and thermoblock. The lids are
powered by Biometra‘s well known
Smart Lid technology with integrated
clutch mechanism which automatically
limits the lid pressure applied to the
plasticware and thus saves tubes from
damage. In addition by formation of
a homogeneous air cushion an even
temperature distribution between
the samples is ensured, significantly
improving temperature uniformity. This
also ensures reproducible conditions
amongst different PCR runs.
The temperature of the independently
working lids can be set individually
in a range between 30 and 99 °C. This
ensures optimal conditions also for
other applications as PCR like e.g.
enzymatic restriction reactions. With
one press on the front button, the lids
gently swing open and arrest in their
end position. In combination with
the now easier to access blocks this
provides a maximum of convenience
to the customer.
USB functions
The TProfessional TRIO Thermocycler is
equipped with two USB ports. The USB
B port serves for the connection of the
thermocycler to a computer and the
USB A port to connect a USB stick.
By using the USB functions it is very
easy to synchronize the memory contents of instruments by the exchange
of PCR-protocols. The USB functions
are the ideal tool for administration of
TProfessional TRIO Thermocyclers and
to expand the memory capacity.
Convenient setup of Temperature
Optimisation Steps
To find the optimal annealing temperature of new primer pairs the
TProfessional TRIO Thermocycler offers
the new Temperature Optimisation
Step function. By making use of the
three independent blocks the
Temperature Optimisation Steps
provide different annealing temperatures in a gradient-like fashion.
In the corresponding screen just enter
an annealing temperature for the block
in the middle and an increment, defining the temperature difference for the
first and third block. For maximum ease
of use programs containing Temperature Optimisation Steps automatically
start on all three blocks so there is
no need to start and stop the blocks
individually. For easy identification
during programming the Temperature
Optimisation Steps are indicated by a
staircase shaped icon (see figure Easy
spreadsheet programming) and corresponding protocols become saved with
the extension “TOS” (see figure Quick
Start of the last five programs).
Temperature Optimisation Step
programming
The annealing temperature defines the
temperature for central block at this step and
the increment the temperatures for the first
and third block.
Order number Item
Block format
Suitable plastic ware
070 - 720
TProfessional TRIO 30
3 x 30 well
0.5 ml tubes
070 - 723
TProfessional TRIO 48
3 x 48 well
0.2 ml tubes, plates, strips
TProfessional TRIO combi
3 x 48 well / 18 * well combi
0.2 ml tubes, plates, strips or
0.5 ml tubes
070 - 724
* capacity increases to 35 x 0.5 ml tubes by use of small cap tubes
Technical Specifications
Practice of patented polymerase chain reaction
(PCR) process requires a license. Please see
Appendix, page 181.
28
Innovation · Advancement · Development
see page 30 – 31
Ordering Information
see page 33 – 34
TH ER MOCYCLER
TProfessional Manager Software
For Controlling TProfessional Thermocycler in a Powerful Network
•
•
•
•
•
Same user interface as
TProfessional Thermocycler
Open, edit and save programs
Copy programs and user directories
MCF – memory cloning function
GLP conform documentation
The TProfessional Manager Software
allows to control up to 5 thermocycler
of the TProfessional family* in a powerful network. PCR runs can be managed
and monitored and versatile memory
management functions allow easy
synchronisation of the connected
TProfessional Thermocyclers. Detailed
run log files provide GLP conform
documentation of PCR runs.
Memory management
The software allows the user to create,
edit and save programs. Single user
directories and programs can be easily
copied and exchanged between the PC
and the TProfessional Thermocylers. The
MCF – memory cloning function allows
to copy the complete memory contents
between different TProfessional
Thermocyclers. By this function all user
directories including programs can be
exchanged.
Documentation
For GLP conform documentation of
PCR runs detailed log-files including
temperature data can be generated
and saved. During the PCR the software
each second records the temperature
data of the block sensors. By the
extended self test the connected
instruments perform a program that
intensively checks all relevant functions.
Moreover Service Info Files can be
created that may serve the Biometra
service department for rapid remote
failure diagnosis if problems occur.
* except TProfessional TRIO
User interface
The TProfessional Manager Software
combines the approved graphical
operating interface of the TProfessional
Series with the comfort of a powerful,
simultaneous PC-controlled network of
up to 5 TProfessional Thermocyclers.
The handling of the TProfessional
Manager is based on the intuitive
operating interface of the thermocyclers
with the choice between for tabular or
graphical programming.
Order number
Item
Ordering Information
070 - 070
TProfessional Manager Software, license file for unrestricted use
see page 33
Innovation · Advancement · Development
29
www.biometra.com
TPersonal Thermocycler
The Personal Cycler with the Maximum Power
• Small footprint
• High throughput
• Fast heating and cooling
Small footprint
The TPersonal is a highly integrated
instrument. With a footprint of only
22 cm x 31 cm the TPersonal occupies
minimum space on your lab bench.
High sample capacity
In the TPersonal 48 a maximum of
48 x 0.2 ml tubes can be run at the
same time. This instrument can also be
used with 48 well microplates or strips.
Three different block formats
The TPersonal comes in three different
block formats. The 48 well version can
be used with 0.2 ml tubes, strips and
48 well microplates. Only few other
Thermocyclers in this segment offer
such a high sample capacity. The 20 well
version is designed for 0.5 ml tubes.
For maximum flexibility we also offer
a combi-block version. The combi-block
holds 48 x 0.2 ml tubes (strips or
microplates) or 18 x 0.5 ml tubes.
High speed
A new class of Peltier elements has
been used in the TPersonal providing
not only fast heating but also fast cooling.
Most conventional Thermocyclers provide
higher heating than cooling rates because
due to thermodynamic principles,
cooling is more energy consuming than
heating. In contrast, due to its improved
technology, the TPersonal is ideally
equipped to ensure specific reaction
conditions and short run times.
Easy to program
Navigation by cursor keys, four function
keys as well as online help make
programming of the TPersonal very easy.
All program parameters can be set in
one spreadsheet without the need to go
through a never-ending flow of different
screens. For maximum convenience
programs can be stored in subdirectories.
In the programming mode as well as
during cycling all important parameters
are displayed in a large backlight graphical
display. The TPersonal Thermocycler
can be controlled in network by the
Thermocycler Manager PC software.
High Performance Smart Lid (HPSL)
The TPersonal is the first personal cycler
featuring the High Performance Smart
Lid technology. The adjustable heated
lid applies a consistent, optimum
pressure, irrespective of the type of
plastic ware used. This ensures a perfect
fit between the tubes and the block,
leading to highly efficient heat transfer.
Due to this automatic control mechanism,
the samples cannot be damaged and
condensation of the reaction mixture is
totally prevented.
Practice of patented polymerase chain reaction
(PCR) process requires a license. Please see
TPersonal Thermocycler
Order number Item
Block format
Suitable plastic ware
050 - 550
TPersonal 20
20 well
0.5 ml tubes
050 - 551
TPersonal 48
48 well
0.2 ml tubes, plates, strips
TPersonal combi
48 well / 18* well combi
0.2 ml tubes, plates, strips or
0.5 ml tubes
050 - 552
* capacity increases to 35 x 0.5 ml tubes by use of small cap tubes
30
Innovation · Advancement · Development
Appendix, page 181.
Technical Specifications
see page 30 – 31
Ordering Information
see page 33 – 34
TH ER MOCYCLER
TRobot Thermocycler
The Perfect Solution for Integration in Robotic Systems
•
•
•
•
Small footprint (17 cm x 13 cm)
Unique: Motorised plate lifter
Motorised heated lid
Easy integration in robotic systems from various suppliers
Optimised for integration in
robotic systems
The TRobot Thermocycler is specifically
designed for integration in Robotic
systems. Due to its minimum footprint
the TRobot ideally fits robotic platforms
where space naturally is limited. The
TRobot is equipped with a motorised
plate lifter which allows the removal of
plates by a robotic arm.
Unique: Motorised plate lifter
Removing plates after thermocycling
is one of the major challenges in
automisation. With conventional
thermocycler systems, the plates
frequently stick to the thermoblock
after thermocycling. Consequently, they
cannot be removed by a robotic arm and
the whole system halts. To overcome
this problem the TRobot has been
equipped with a patented motorised
plate lifter. This lifter elevates the plate
from the block as the lid opens. Once
lifted, the plate can be easily removed
by the robotic arm.
Two different block models
The TRobot is available in two different
block versions for 96 well and 384 well
plates. Taking advantage of the high
thermal conductivity of silver the
TRobot 96 achieves high ramping rates
as well as excellent temperature
uniformity. The TRobot 384 achieves
perfect fit for 384 well plates. The 384
well block is coated with a special alloy
to facilitate plate removal by a robotic
arm.
Software integration
In a robotic environment the TRobot is
controlled by the PC of the robot. Integration of the Biometra Thermocycler
Manager software allows quick access
to all thermocycler functions. Alternatively, the TRobot control can be directly
implemented into the control software
of the robotic system. For this purpose a
comprehensive description of the serial
communication commands is available.
TRobot Thermocycler
Order number Item
Block format
Suitable plastic ware
050 - 991
TRobot 96
96 well
96 well plates (0.2 ml tubes, strips)
Technical Specifications
050 - 992
TRobot 384
384 well
384 well plates
see page 30 – 31
Practice of patented polymerase chain reaction (PCR) process requires a license.
Please see Appendix, page 181.
Ordering Information
see page 33 – 34
Innovation · Advancement · Development
31
www.biometra.com
Thermocycler
Technical Specifications
Features
Block exchangeable
Block formats
Program memory
Temperature range
Temperature gradient*
Cool samples to 4 °C
Maximum heating
TProfessional
TProfessional Standard
TProfessional Basic
High Speed
Large graphical Display
Exchangeable Blocks
Gradient version available
High Speed
Large graphical Display
Gradient version available
Large graphical Display
Easy Spreadsheet
programming
Gradient version available
Yes
-
-
60 well
96 well
384 well
60 well
96 well
384 well
96 well
96 well XL
350 average programs
350 average programs
350 average programs
3 – 99 °C
3 – 99 °C
3 – 99 °C
40 °C
40 °C
26 °C
Yes
Yes
Yes
6.0 °C / sec
6.0 °C / sec
3.5 °C / sec
Maximum cooling
4.5 °C / sec
4.5 °C / sec
2.5 °C / sec
Average heating
5.0 °C / sec
5.0 °C / sec
3.0 °C / sec
Average cooling
3.5 °C / sec
3.5 °C / sec
2.0 °C / sec
± 0.15 °C at 55 °C
± 0.25 °C at 70 °C
± 0.50 °C at 95 °C
± 0.15 °C at 55 °C
± 0.25 °C at 70 °C
± 0.50 °C at 95 °C
± 0.20 °C at 55 °C
± 0.30 °C at 70 °C
± 0.60 °C at 95 °C
± 0.1 °C
± 0.1 °C
± 0.1 °C
¼ VGA screen,
320 x 240 pixel
¼ VGA screen,
320 x 240 pixel
¼ VGA screen,
320 x 240 pixel
Yes
Yes
Yes
Easy Spreadsheet
Programming (ESP)
Graphical programming
Easy Spreadsheet
Programming (ESP)
Graphical programming
Easy Spreadsheet
Programming (ESP)
Toggle between spreadsheet
and graphical mode,
gradient temperature graph*,
adjustable ramp rates,
extended self test, service files,
automatic block recognition,
PC control by TProfessional
Manager Software
Toggle between spreadsheet
and graphical mode,
gradient temperature graph*,
adjustable ramp rates,
extended self test, service files,
PC control by TProfessional
Manager Software
Adjustable ramp rates,
extended self test,
service files,
PC control by TProfessional
Manager Software
Yes
Yes
Yes
Temperature Uniformity
(15 sec after clock starts)
Control accuracy
Display
Auto restart after power failure
Programming modes
Software options
Quick start of the last
5 programs
High Precision Smart Lid
Yes
Yes
Yes
30 – 99 °C
30 – 99 °C
30 – 99 °C
Max. power consumptiom
480 Watt
480 Watt
480 Watt
Noise emission
Very low
Very low
Very low
RS232 serial port
RS232 serial port
RS232 serial port
28 cm x 38 cm x 24 cm
28 cm x 38 cm x 24 cm
28 cm x 38 cm x 24 cm
Lid temperature range
Interfaces
Dimensions (W x D x H)
* Applies to models with gradient feature
32
Innovation · Advancement · Development
TH ER MOCYCLER
Features
Block exchangeable
Block formats
Program memory
Temperature range
Temperature gradient*
TProfessional TRIO
TPersonal
TRobot
Three independent blocks
Large graphical display
High parallel thoughput
Compact footprint
Compact footprint
High capacity
Motorised lid
Plate lifter
-
-
-
30 well
48 well
combi
20 well
48 well
combi
96 well
384 well
350 average programs
250 average programs
250 average programs;
unlimited on PC
3 – 99 °C
3 – 99 °C
- 3 – 99 °C
Temperature Optimisation Step
-
-
Yes
Yes
Yes
Maximum heating
5.0 °C / sec
n.d.
n.d.
Maximum cooling
4.5 °C / sec
n.d.
n.d.
Average heating
4.2 °C / sec
3 °C / sec
3.5 °C / sec
Average cooling
3.8 °C / sec
3 °C / sec
2.5 °C / sec
+/- 0.20 °C at 50 °C
+/- 0.30 °C at 70 °C
+/- 0.60 °C at 95 °C
± 0.5 °C
± 0.5 °C
± 0.1 °C
± 0.1 °C
± 0.1 °C
¼ VGA Screen,
320 x 240 pixel
High brightness CFL backlight
graphical LC Display,
256 x 64 dots
-
Yes
Yes
Yes
Easy Spreadsheet
Programming (ESP)
Graphical programming
Spreadsheet Programming
PC software
Toggle between easy spreadsheet
and graphical mode, adjustable
ramp rates, time and temperature increments, extended self
test, service info files, Temperature Optimsation Step (TOS),
versatile USB functions for
exchange of PCR-protocols
Adjustable ramp rates,
time increments,
temperature increments,
PC control by Thermocycler
Manager Software
Adjustable ramp rates,
time increments,
temperature increments,
PC control by Thermocycler
Manager Software
Quick start of the last
5 programs
Yes
-
-
High Precision Smart Lid
Yes
Yes
Yes
Lid temperature range
30 – 99 °C
30 – 99 °C
30 – 99 °C
Max. power consumptiom
1000 Watt
380 Watt
350 Watt
Cool samples to 4 °C
Temperature Uniformity
(15 sec after clock starts)
Control accuracy
Display
Auto restart after power failure
Programming modes
Software options
Noise emission
Interfaces
Dimensions (W x D x H)
Very low
Very low
Very low
USB B, USB A
RS232 serial port
RS232 serial port
30 cm x 38 cm x 19 cm
22 cm x 31 cm x 15 cm
17 cm x 23 cm x 20 cm
* Applies to models with gradient feature
Innovation · Advancement · Development
33
www.biometra.com
Thermocycler Capacity and Suitable Consumables
Thermocycler
Consumable
Tubes
0.2 ml
Tubes
0.5 ml
Strips
8 x 0.2 ml
Plates
48 well
Plates
96 well
Plates
96 well
Plates
384 well
(white, for qPCR)
Order No.
050 - 310
050 - 320
050 - 214
050 - 215
050 - 225
050 - 232
070 - 800
-
60
-
-
-
-
TProfessional Gradient 96
070 - 801
96
-
12
2
1
-
TProfessional 60
070 - 900
-
60
-
-
-
-
TProfessional Gradient 60
050 - 240
TProfessional 96
070 - 901
96
-
12
2
1
-
TProfessional 384
070 - 902
-
-
-
-
-
1
TProfessional Standard
Gradient 60
070 - 850
-
60
-
-
-
-
TProfessional Standard
Gradient 96
070 - 851
96
-
12
2
1
-
TProfessional Standard
Gradient SL 96
070 - 871
96
-
12
2
1
-
TProfessional Standard 60
070 - 950
-
60
-
-
-
-
TProfessional Standard 96
070 - 951
96
-
12
2
1
-
TProfessional Standard
SL 96
070 - 971
96
-
12
2
1
-
TProfessional Standard 384
070 - 952
-
-
-
-
-
1
TProfessional Basic 96
070 - 701
96
-
12
2
1
-
TProfessional Basic XL 96
070 - 702
96
-
12
2
1
-
TProfessional Basic
Gradient 96
070 - 601
96
-
12
2
1
-
TProfessional Basic
Gradient XL 96
070 - 602
96
-
12
2
1
-
TProfessional TRIO 30
070 - 720
-
3 x 30
-
-
-
-
TProfessional TRIO 48
070 - 723
3 x 48
-
3 x 6
3 x 1
-
-
TProfessional TRIO combi
070 - 724
3 x 48
3 x 18 *
3 x 6
3 x 1
-
-
TPersonal 20
050 - 550
-
20
-
-
-
-
TPersonal 48
050 - 551
48
-
6
1
-
-
TPersonal combi
050 - 552
48
18*
6
1
-
-
TRobot 96
050 - 991
(96)
-
(12)
(2)
1
-
TRobot 384
050 - 992
-
-
-
-
-
1
TOptical Gradient 96
070 - 500
1
TOptical 96
070 - 501
1
* capacity increases to 35 x 0.5 ml tubes by use of small cap tubes
34
050-259
Innovation · Advancement · Development
TH ER MOCYCLER
Thermocycler and Block Modules
Order Information
Item
Order No.
TOptical Gradient 96 for 96 tubes, strips or 96 well microplates
070 - 500
TOptical 96 for 96 tubes, strips or 96 well microplates
070 - 501
TProfessional Basic Gradient for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 601
TProfessional Basic Gradient XL for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 602
TProfessional Basic for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 701
TProfessional Basic XL for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 702
TProfessional Standard Gradient 60 for 60 tubes (0.5 ml)
070 - 850
TProfessional Standard Gradient 96 for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 851
TProfessional Standard Gradient SL 96 for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 871
TProfessional Standard 60 for 60 tubes (0.5 ml)
070 - 950
TProfessional Standard 96 for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 951
TProfessional Standard SL 96 for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 971
TProfessional Standard 384 for 384 well microplates
070 - 952
TProfessional Gradient 60 for 60 tubes (0.5 ml)
070 - 800
TProfessional Gradient 96 for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 801
TProfessional 60 for 60 tubes (0.5 ml)
070 - 900
TProfessional 96 for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 901
TProfessional 384 for 384 well microplates
070 - 902
TProfessional TRIO 30 for 3 x 30 tubes (0.5 ml)
070 - 720
TProfessional TRIO 48 for 3 x 48 tubes (0.2 ml)
070 - 723
TProfessional TRIO combi for 0.5 ml tubes (3 x 18) or 0.2 ml tubes (3 x 48)
070 - 724
TProfessional Manager Software
070 - 070
TPersonal 20 for 20 tubes (0.5 ml)
050 - 550
TPersonal 48 for 48 tubes (0.2 ml)
050 - 551
TPersonal combi for 18 x 0.5 ml or 48 x 0.2 ml tubes
050 - 552
TRobot 96 Thermocycler for [96 tubes (0.2 ml), strips or] 96 well microplates
050 - 991
TRobot 384 Thermocycler for 384 well microplates
050 - 992
Block modules
TOptical module Gradient 96 for 96 tubes, strips or 96 well microplates
070 - 510
TOptical module 96 for 96 tubes, strips or 96 well microplates
070 - 511
TProfessional module Gradient 60 for 60 tubes (0.5 ml)
070 - 810
TProfessional module Gradient 96 for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 811
TProfessional module 60 for 60 tubes (0.5 ml)
070 - 910
TProfessional module 96 for 96 tubes (0.2 ml), strips or 96 well microplates
070 - 911
TProfessional module 384 for 384 well microplates
070 - 912
Innovation · Advancement · Development
35
www.biometra.com
Thermocycler Accessories
Order Information
Item
Order No.
Temperature verification
Portable system for temperature verification of Thermocyclers (PTMD Type 700)
inclusive 1 sensor for 0.2 ml and 1 sensor for 0.5 ml
050 - 420
Sensor for 0.5 ml well (PTMD), Type 600/700
050 - 416
Sensor for 0.2 ml well (PTMD), Type 600/700
050 - 417
Sensor for 384 well (PTMD), Type 600/700
050 - 418
Portable system for temperature verification of Thermocyclers (PTMD Type 700) inclusive 1 sensor for 384 well
050 - 421
PC-Software for PTMD type 700
050 - 422
Sensors for older versions of PTMDs
Sensor for 0.5 ml well (PTMD), Type 500 / 505
050 - 411
Sensor for 0.2 ml well (PTMD), Type 500 / 505
050 - 412
Sensor for 384 well (PTMD), Type 500 / 505
050 - 413
Consumables
Microplates
48 well microplate, 50 pcs.
050 - 225
96 well microplate Polypropylen, full skirted, 25 pcs.
050 - 232
96 well microplate, non skirted, 25 pcs.
050 - 253
96 well microplate Polypropylen, non skirted, low-profile, 25 pcs.
050 - 213
96 well skirted, white 50 pcs.
050 - 259
384 well microplate, 50 pcs.
050 - 240
384 well microplate HSQ, 50 pcs.
050 - 231
Tubes and strips
0.2 ml tubes, 1.000 pcs. transparent, with caps
050 - 310
0.5 ml tubes, 1.000 pcs. transparent, with caps
050 - 320
250 strips 0.2 ml 8 tubes and 8 flat caps each
050 - 254
250 strips 0.2 ml 8 tubes and 8 domed caps each
050 - 255
Microplate sealing
36
Silicone mat, 50 pcs.
050 - 237
Adhesive sealing film, 100 pcs.
050 - 250
Optical adhesive film, 100 pcs.
050 - 258
Nop mat 96 well, 20 pcs.
050 - 251
Nop mat 384 well, 20 pcs.
050 - 252
Innovation · Advancement · Development
TH ER MOCYCLER
Oligonucleotides
Extensive Portfolio that Meets all Possible Needs
Available in Germany only
•
•
•
•
•
DNA- and RNA oligonucleotides
More than 150 fluorescent labels
and 40 modifications
High coupling efficiencies
Also synthesis of very long
oligonucleotides or synthesis of
large quantities
Fast delivery times
DNA oligonucleotides
DNA oligonucleotides are offered in
different synthesis quantities and
different purification grades.
The products are dissolved in sterile
water and adjusted to a concentration
of 0.1 nmol / µl. For all synthesis setup
fees and shipping costs are already
included.
Standard synthesis
(Mol. Biology Grade.)
• Efficient removal of
non-incorporated nucleotides
• Deprotected and desalted
Synthesis scale
Order No.
0.05 µmol
120 - 000
0.20 µmol
120 - 100
1.00 µmol
120 - 200
Larger synthesis quantities on request.
Guaranteed minimum
delivery quantity
Synthesis scale
Minimum delivery
quantity
0.05 µmol
5 nmol
0.20 µmol
25 nmol
1.00 µmol
100 nmol
Modifications:
For oligonucleotides a wide range
of modifications is available. More
than 150 different color labels can be
offered. Moreover 5'-modifications
by non-nucleoside components such
as amino, thio-linker or phosphates,
3'-modifications or internal modifications
by nucleoside components can
be provided. All modifications of
oligonucleotides are inclusive a 2 fold
HPLC purification (1x for synthesis,
1x after coupling of the modification) at
no extra charge.
Order No.
Order No.
Order No.
Modification*
0.05 µmol scale
0.2 µmol scale
1.0 µmol scale
5'-Phosphate
121 - 000
126 - 000
121 - 200
5'-Aminolink
121 - 002
126 - 002
125 - 002
5'-Thiolink
121 - 005
126 - 005
125 - 005
5'-Biotin
121 - 007
126 - 007
121 - 207
3'-Biotin
121 - 009
126 - 009
125 - 009
5'-Digoxigenin**
121 - 010
126 - 010
125 - 010
5'-Fluorescein (5-FAM)
121 - 012
126 - 012
125 - 012
5'-Fluorescein (6-FAM)
121 - 030
126 - 030
125 - 030
3'-Fluorescein
121 - 013
126 - 013
125 - 013
5'-Rhodamine (5-TAMRA)
121 - 014
126 - 014
125 - 014
5'-Rhodamine (6-TAMRA)
121 - 031
126 - 031
125 - 031
5'-Texas Red
121 - 048
126 - 048
125 - 048
5'-ROX (6-ROX)
121- 033
126 - 033
125 - 033
5'-Cy 5
121 - 034
126 - 034
125 - 034
5'-Cy 3
121 - 035
126 - 035
125 - 035
5'-HEX
121 - 036
126 - 036
125 - 036
5'-Tet
121 - 060
126 - 060
125 - 060
JOE
121 - 038
126 - 038
125 - 038
Rhodamine Green
121 - 039
126 - 039
125 - 039
dInosine
121 - 016
126 - 016
125 - 016
dUridine
121 - 017
126 - 017
125 - 017
dT-Aminolink
121 - 028
126 - 028
125 - 028
3' BHQ 493nm
121 - 070
127 - 070
125 - 070
5' BHQ 493nm
121 - 071
127 - 071
125 - 071
3' BHQ 534nm
121 - 072
127 - 072
125 - 072
5' BHQ 534nm
121 - 073
127 - 073
125 - 073
3' BHQ 579nm
121 - 074
127 - 074
125 - 074
5' BHQ 579nm
121 - 075
127 - 075
125 - 075
3' BHQ 672nm
121 - 076
127 - 076
125 - 076
5' BHQ 672nm
121 - 077
127 - 077
125 - 077
* additional modifications available on request
** licensed from Boehringer Mannheim.
Innovation · Advancement · Development
37
www.biometra.com
Purification and documentation
The synthesis of oligonucleotides
includes a standard Mol. Biol. Grade
purification (see Page 37). By the standard purification salts, base protecting
groups and short failure sequences up
to 6 bases are removed efficiently.
For advanced demands also HPLC- and
HPLC-Purification (PCR-Grade)
• Efficient removal of
non-incorporated nucleotides
• More than 95 % full-length
products guaranteed
Cell Culture Grade:
• Efficient removal of
non-incorporated nucleotides
• More than 95 % full-length
products guaranteed
• Two HPLC purifications, sterile filtration
cloning, PCR mutagenesis, probes,
modified oligonucleotides and
oligonucleotides longer than 40 bases.
Cell Culture Grade delivers the highest
quality including two HPLC purification
steps and one sterile filtration step.
For quality documentation additionally
to the synthesis documentation
a quality protocol can be delivered.
Synthesis scale
Order No.
Synthesis scale
Order No.
Item
Order No.
0.05 µmol
120 - 002
0.05 µmol
120 - 003
Analytical HPLC
120 - 400
0.20 µmol
120 - 102
0.20 µmol
120 - 103
120 - 202
1.00 µmol
120 - 203
PolyacrylamideGelelectrophoresis
120 - 500
1.00 µmol
RNA oligonucleotides:
For the synthesis of long RNAs, labeled
RNAs, 2‘- and base modified RNAs,
triphosphates, modified dinucleotides
and dsRNAs a variety of new synthesis
methods were developed to ensure a
high yield and biological activity to the
RNA oligonucleotides.
As a consequence high-quality synthesis
products, especially also long RNA
molecules can be are offered, which
are further more protected against
degradation by nucleases.
38
Cell Culture Grade purification are
offered. Apart from salts and protecting
groups by the HPLC step trityl-off failure
sequences are depleted.
The HPLC purification is recommended
whenever full-length or high
reproducibility are essential as for
Typical examples of RNA applications
include:
• dsRNA for gene silencing
• Chimeric DNA / RNA
• Ribozymes
• Investigation of ribozyme-substrate
interactions
• Investigation of tRNA functions
• RNA footprinting to study RNA
protein interaction
• Aptamers for spezific inhibition of
protein functions
Order No.
Order No.
Item
0.2 µmol scale
1.0 µmol scale
Double stranded RNA
126 - 090
126 - 091
Standard RNA modification
126 - 074
126 - 075
ASPTO
126 - 070
126 - 071
RNA-ASPTO-modification
126 - 072
126 - 073
2'-5'- RNA modification
126 - 076
126 - 077
5-Methyl Uridine (rT) modification
—
126 - 078
5-C6 Amino Uridine modification
126 - 079
126 - 080
2-Amino Ribo Purine modification
126 - 081
126 - 082
2'-Amino RNA modification
126 - 083
126 - 084
2'-Fluoro RNA modification
—
126 - 085
2'-O-Me-RNA
126 - 086
126 - 087
Inosine-RNA
126 - 088
126 - 089
Innovation · Advancement · Development
• Synthesis of RNA for in-vitro
translation experminents
• RNA for in vivo investigations
Larger synthesis quantities on request,
further modifications can be performed
on request.
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