Manual v1.3

Manual v1.3
3
Prime, 3PrimeX and 3PrimeG
OPERATOR’S MANUAL
Version 1.3
11/2012
CONTENTS
INTRODUCTION
BEFORE USE
Unpacking
SAFETY INFORMATION
English
Français
Deutsch
Italiano
Español
CONTACT INFORMATION
3
PRIME SPECIFICATION
INSTALLATION
GENERAL SOFTWARE FEATURES
Introduction
Template programs
Incremented/decremented time and temperature
3
Gradient ( PrimeG only)
Oligonucleotide Tm calculator
PROGRAMMING TEXT, TEMPERATURE AND TIME
Text entry
Temperature entry
Time entry
INSTRUMENT SETTINGS AND DEFAULTS
User preferences
Instrument settings
Program defaults
Service settings
Gradient calculator
Oligonucleotide Tm calculator
CREATE A NEW PROGRAM
Insert/delete a stage
Insert/delete a step
Insert/delete a pause
The More button
Program options
Settings
RUN A PROGRAM
Run
Edit
Copy
Delete
Search
3
UPDATING THE PRIME SOFTWARE
3
3
UPGRADING FROM PRIME TO PRIMEG
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PAGE
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40
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42
43
44
45
46
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48
FAQs
TECHNICAL SUPPORT AND SERVICING
ADDITIONAL INFORMATION
User Maintenance
Fault Finding
Fuses
Insulation Testing
ACCESSORIES
REPLACEMENT PARTS
DECLARATION OF CONFORMITY
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2
INTRODUCTION
3
The Prime range is a family of personal thermal cyclers designed to accurately control the
temperatures of samples between 4ºC and 100°C. They have many scientific applications including
3
PCR, reverse transcription, ligation and sequencing. The basic Prime unit can accommodate 24 x
3
0.2ml tubes in a flexible 8 x 3 array or 15 x 0.5ml tubes. The PrimeX has either a 48 x 0.2ml or 30 x
3
0.5ml block and can easily be upgraded to the PrimeG which incorporates a block gradient function
3
and gradient calculator, allowing for simple and rapid optimisation of reaction conditions. The PrimeX
and G also have a fully adjustable heated lid to accommodate a wide range of different consumables.
3
The Prime units are programmed via a 3.5” colour VGA LCD touch screen using simple and intuitive
software. Programs and run progress can be viewed in real time, allowing the user to readily check the
status or position within a run. A USB port on the front of the unit allows transfer of files to and from a
USB memory stick for viewing in the Techne Workbench PC software and for the easy update of
instrument software.
3
BEFORE USE
3
Before using the Prime please ensure you have read this manual carefully. If there is any doubt
relating to the proper use of this equipment, the staff at Bibby Scientific Ltd. or your supplier will be
happy to assist you.
UNPACKING
When unpacking the unit please ensure that the following have been removed from the packaging:
•
3
Prime with block
•
Mains cables (UK, EU and US)
•
Operator’s manual
•
Warranty Registration card
The user is advised to keep the original packaging in case the instrument ever needs to be returned
for service or repair. Bibby Scientific Ltd. accepts no responsibility for damage incurred unless the
unit is correctly packed and transported in its original packaging.
4
SAFETY INFORMATION
3
Please read all the information in this manual before using the Prime.
WARNING
HIGH TEMPERATURES ARE DANGEROUS: they can cause serious burns to operators and ignite
combustible material. Users should be aware of the following potential hazards:
•
USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS
•
DO NOT use combustible substances near hot objects
•
DO NOT operate the instrument in the vicinity of inflammable liquids or gases
•
DO NOT place any liquid directly into the instrument.
OPERATOR SAFETY
All operators of Techne equipment must have available the relevant literature needed to ensure their
safety. It is important that only suitably trained personnel operate this equipment, in accordance with
the instructions contained in this manual and with general safety standards and procedures. If the
equipment is used in a manner not specified by Bibby Scientific Ltd. the protection provided by the
equipment to the operator may be impaired.
All Techne instruments are designed to conform to international safety requirements and are fitted
with an over-temperature cut-out. If a safety problem should be encountered, switch off the unit at the
mains socket and remove the plug from the electricity supply.
INSTALLATION
The instrument should be carried using both hands. Never move or carry the instrument when in use
or connected to the mains electricity supply.
1. All Techne instruments are supplied with a power cable; this may be integral or plug-in.
2.
Before connecting the instrument to the mains electricity supply, check the voltage
against the rating plate (located on the back of the unit). Please note that the unit
must be earthed to ensure proper electrical safety. Connect the mains cable to a
suitable plug according to the table below.
Connections
220/240V, 50/60Hz
110V/120V, 50/60Hz
Live
Brown
Black
Neutral
Blue
White
Earth
Green/yellow
Green
3. UK ONLY: The fused plug supplied with the mains cable is fitted with a 5 amp fuse to protect the
instrument and the operator.
4. The units are rated to operate at 100-240V, 50/60Hz.
5. Place the unit on a suitable flat bench or in a fume cupboard if required, ensuring that the air inlet
vents on the underside are free from obstruction.
6. Plug the mains cable into the socket on the back of the instrument.
7. Switch on the instrument using the switch located on the back of the unit.
Replacement cable
2
Should the mains lead need replacement, a cable of 1mm of harmonized code H05VV-F connected
to an IEC320 plug should be used. IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN.
5
ENVIRONMENTAL CONDITIONS
The
•
•
•
•
•
•
•
3
Prime is designed operate under the following conditions:
Indoor use
Ambient temperature range +5ºC to +40ºC
Altitude to 2000m
Relative humidity not exceeding 80%
Mains supply fluctuations not exceeding 10%
Over voltage category II IEC 60364-4-443
Pollution degree 2
Note: The control specifications are quoted at an ambient temperature of 20ºC. The specification
may deteriorate outside an ambient temperature of between 10ºC and 30ºC.
The instrument has been tested for radio frequency interference and is certified under EN61326.
WARRANTY
Bibby Scientific Ltd warrants this instrument to be free from defects in material and workmanship,
when used under normal laboratory conditions for four (4) years. In the event of a justified claim,
Bibby Scientific Ltd will replace any defective component or replace the unit free of charge. This
warranty does not apply if damage is caused by fire, accident, misuse, neglect, incorrect adjustment
or repair, damage by incorrect installation, adaption, modification, fitting of non-approved parts or
repair by unauthorised personnel. Bibby Scientific Ltd liability is limited to the cost of repair or
replacement of the product and excludes in particular, indirect and consequential loss, damage,
costs or expenses, including but not limited to wasted time, materials and expenditure or loss of use,
profit, production, revenue, expected savings or goodwill. To make a claim please contact the
supplier of the instrument. This warranty is in addition to, and does not affect any statutory rights.
This manual has been prepared for the convenience of Techne’s customers and nothing in this
manual shall be taken as a warranty, condition or representation concerning the description,
merchantability, fitness for purpose or otherwise of the unit or components.
Notwithstanding the description and specification(s) of the instruments contained in the operator’s
manual, Techne reserves the right to make such changes as it sees fit to the instruments or to any of
the components.
6
L'INFORMATION DE SÛRETÉ
3
Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le Prime.
AVERTISSEMENT
Les TEMPÉRATURES ÉLEVÉES SONT DANGEREUSES car elles peuvent provoquer de graves
brûlures chez l’opérateur et enflammer les matériaux combustibles. Les utilisateurs doivent porter
une attention toute particulière aux points suivants :
•
PROCEDER AVEC PRUDENCE ET PORTER DES GANTS POUR SE
PROTEGER LES MAINS
•
NE PAS utiliser de matériaux combustibles auprès d’objets chauds.
•
NE PAS utiliser l’appareil à proximité de liquides ou de gaz inflammables
•
NE PAS verser de liquides directement dans l’appareil.
SECURITE DE L’OPERATEUR
Tous les utilisateurs de produits Techne doivent avoir pris connaissance des consignes et instructions
nécessaires à la garantie de leur sécurité. Important: cet appareil doit impérativement être manipulé
par un personnel qualifié et être utilisé selon les instructions données dans ce document, en accord
avec les normes et procédures de sécurité générales. Dans le cas où cet appareil ne serait pas utilisé
selon les consignes précisées par Bibby Scientific Ltd., la protection pour l’utilisateur ne serait alors
plus garantie.
Tous les appareils Techne sont conçus pour répondre aux normes de sécurité internationales et sont
dotés d’un coupe-circuit en cas de surchauffe. En cas de problème de sécurité, couper l’alimentation
électrique au niveau de la prise murale et enlevez la prise connectée à l’appareil.
INSTALLATION
Porter l'appareil à deux mains. Ne jamais déplacer ou transporter l’appareil lorsqu’il est en
fonctionnement ou branché à l’alimentation électrique.
1. Tous les appareils Techne sont livrés avec un câble d'alimentation, qui peut être intégré à l'appareil
ou à raccorder.
2.
Avant de raccorder l’appareil à l’alimentation électrique sur secteur, vérifier la tension
requise indiquée sur la plaque d’identification (située au dos de l’appareil). Il est
important que l’appareil soit relié à la terre pour assurer la protection électrique
requise. Brancher le câble secteur sur une prise appropriée, voir tableau ci-après.
Connexions
220/240 V, 50/60 Hz
110/120 V, 50/60 Hz
Phase
Marron
Noir
Neutre
Bleu
Blanc
Terre
Vert/jaune
Vert
3. ROYAUME-UNI SEULEMENT: La prise avec fusible intégré fournie avec le câble secteur est
munie d’un fusible 5 A destiné à protéger l’appareil et l’utilisateur.
4. Les unités sont évaluées pour fonctionner à 100-240V, 50/60Hz.
5. Placer l’appareil sur une surface plane ou si nécessaire sous une hotte d’aspiration, veiller à ce
que les trous d’aération situés sous l’appareil ne soient pas obstrués.
6. Raccorder le câble d’alimentation à la prise située à l’arrière de l’appareil.
7. Allumer l'appareil utilisant le commutateur situé à l'arrière de l'unité.
7
Câble de rechange
S’il s’avère nécessaire de remplacer le cordon d’alimentation, utiliser un câble de 1 mm² conforme à
la norme H05VV-F relié à une prise IEC320. EN CAS DE DOUTE, CONSULTER UN ELECTRICIEN
QUALIFIE.
CONDITIONS ENVIRONNEMENTALES
3
Le Prime est conçu pour fonctionner dans les conditions suivantes:
•
Pour un usage intérieur seulement
•
Température ambiante +5°C à +40°C
•
Altitude inférieure à 2000m
•
Humidité relative ne dépassant pas 80%
•
Fluctuations de l’alimentation n’excédant pas 10% de la valeur nominale
•
Catégorie II IEC 60364-4-443 de surtension
•
Degré de pollution 2
Remarque: Les paramètres sont indiqués pour une température ambiante de 20°C. Ces
caractéristiques peuvent se détériorer en dehors d’une température ambiante de 10 à 30°C.
L’appareil a été testé en matière de radiofréquences et est certifié selon la norme EN61326.
GARANTIE
Bibby Scientific Ltd garantit cet instrument contre tout vice de matière et de fabrication, quand il est
utilisé dans des conditions normales de laboratoire pendant quatre (4) ans. En cas de réclamation
justifiée, Bibby Scientific Ltd procèdera gratuitement au remplacement de tout élément défectueux ou
au remplacement de l'appareil. Cette garantie ne s'applique pas aux dommages provoqués par les
incendies, accidents, mésusages, négligences, réglages ou réparations incorrects, aux dommages
dus à une installation incorrecte, adaption, modification, au montage de pièces non homologuées ou
réparation par un personnel non autorisé. La responsabilité de Bibby Scientific Ltd se limite aux
coûts de réparation ou de remplacement du produit et exclut, en particulier, les pertes, dommages,
coûts ou dépenses indirects et conécutifs, parmi lesquels, mais sans s'y limiter, les pertes de temps,
de matériaux et les dépenses ou perte d'utilisation, de profit, de production, de chiffre d'affaires,
d'économies escomptées ou écarts d'acquisition. En cas de réclamation, veuillez prendre contact
avec le fournisseur de cet appareil. Cette garantie s'ajoute à vos droits légaux et n'affecte en rien ces
derniers.
Le présent manuel a été exclusivement rédigé à l’attention des clients de la marque Techne et rien
dans son contenu ne doit être pris comme une garantie, une condition ou une affirmation concernant
la description, la commercialisation, l’adéquation à un usage particulier de l'appareil ou de ses
composants.
Malgré la description et les caractéristiques techniques des appareils données dans le manuel de
l’utilisateur, la société Techne se réserve le droit d’apporter les changements nécessaires à l’appareil
ou à tout élément qui entre dans sa composition.
8
SICHERHEITS- INFORMATIONEN
3
Lesen Sie diese Anleitung vor Verwendung des Prime bitte sorgfältig durch.
ACHTUNG
HOHE TEMPERATUREN STELLEN EINE GEFAHRENQUELLE DAR. Sie können schwere
Brandverletzung verursachen und brennbare Stoffe entzünden. Der Benutzer sollt sich mit den
möglichen Gefahren vertraut machen:
•
UMSICHTIG VORGEHEN UND SCHUTZHANDSCHUHE TRAGEN
•
KEINE brennbaren Stoffe in der Nähe heißer Gegenstände verwenden
•
Das Gerät NICHT in der Nähe entzündlicher Flüssigkeiten oder Gase betreiben
•
Flüssigkeiten NICHT direkt auf das Gerät auftragen.
SICHERHEIT DES BEDIENPERSONALS
Alle Benutzer von Techne Geräten müssen Zugang zu der entsprechenden Literatur haben, um ihre
Sicherheit zu gewähren. Es ist wichtig, daß diese Geräte nur von entsprechend geschultem Personal
betrieben werden, das die in dieser Gebrauchsanweisung enthaltenen Maßnahmen und allgemeine
Sicherheitsbestimmungen und - vorkehrungen beachtet. Wenn das Gerät anders eingesetzt wird als
vom Hersteller empfohlen, kann dies die persönliche Sicherheit des Anwenders beeinträchtigen.
Die Geräte von Techne entsprechen den internationalen Sicherheitsbestimmungen und sind mit einem
automatischen Übertemperaturabschalter ausgestattet. Wenn ein Sicherheitsproblem auftreten sollte,
muß das Gerät ausgeschaltet und vom Stromnetz getrennt werden.
INBETRIEBNAHME
Das Gerät mit beiden Händen tragen. Das Gerät unter keinen Umständen transportieren, wenn es in
Betrieb ist, oder während das Gerät noch am Netz angeschlossen ist.
1.
Alle Geräte von Techne werden mit einem Netzkabel geliefert, das entweder eingesteckt wird
oder fest mit dem Gerät verbunden ist.
2.
Vor dem Anschluss bitte kontrollieren, ob die Stromversorgung den Angaben auf dem
Typenschild (auf der Geräterückseite) entspricht. Um die elektrische Sicherheit zu
gewährleisten, muss dieses Gerät geerdet werden. Schließen Sie das Netzkabel
entsprechend der folgenden Tabelle an einen geeigneten Stecker an.
Anschluss
220/240V, 50/60Hz
110V/120V, 50/60Hz
Phase
Braun
Schwarz
Neutral
Blau
Weiß
Erde
Grün/Gelb
Grün
3.
NUR FÜR GROSSBRITANNIEN: der mit dem Netzkabel gelieferte Sicherungsstecker enthält
eine 5 Amp. Sicherung zum Schutz des Geräts und des Anwenders.
4.
Die Maßeinheiten werden veranschlagen, um an 100-240V, 50/60Hz zu funktionieren.
5.
Stellen Sie das Gerät auf einen geeigneten ebenen Tisch oder in einem Abzugsschrank auf und
sorgen Sie dafür, dass die Lufteinlassschlitze auf der Geräteunterseite nicht blockiert sind.
6.
Stecken Sie das Netzkabel in die Buchse auf der Geräterückseite ein.
7.
Schalten Sie das Instrument unter Verwendung des Schalters an, der auf der Rückseite der
Maßeinheit gelegen ist.
9
Ersatzkabel
2
Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1 mm
Adernquerschnitt und Europastecker (IEC 320) benötigt. IM ZWEIFELSFALL EINEN ELEKTROFACHMANN HINZUZIEHEN.
UMWELTBEDINGUNGEN
Der
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•
•
•
•
•
•
3
Prime ist für den Einsatz unter folgenden Bedingungen ausgelegt:
Gebrauch in Innenräumen
Umgebungstemperatur zwischen +5ºC to +40ºC
Höhe: bis zu 2000 m
Relative Feuchte nicht über 80%
Netzspannungsschwankungen nicht über 10%
Überspannungsklasse 2 IEC 60364-4-443
Verschmutzungsgrad 2
Hinweis: Die Gerätespezifikationen beziehen sich auf eine Umgebungstemperatur von 20ºC und
können sich außerhalb des Bereichs 10ºC bis 30ºC verschlechtern.
Das Gerät wurde auf HF-Störeinflüsse geprüft und entspricht den EMV-Bedingungen nach EN61326.
GARANTIE
Bibby Scientific Ltd gewährleistet, dass dieses Gerät vier (4) Jahre lang keine Material- oder
Herstellungsfehler aufweist, wenn es unter normalen Laborbedingungen verwendet wird. In einem
berechtigten Garantiefall wird Bibby Scientific Ltd alle defekten Komponenten oder das Gerät
kostenlos austauschen. Diese Garantie gilt nicht, wenn der Schaden durch ein Feuer, einen Unfall,
Missbrauch, Nachlässigkeit, falsche Einstellungen oder Reparaturen, falsche Installation,
Adaptierung, Veränderung, den Einbau nicht zugelassener Teile oder die Reparatur durch nicht
autorisiertes Personal verursacht wurde. Die Garantie von Bibby Scientific Ltd ist beschränkt auf die
Kosten der Reparatur oder den Austausch des Produkts und schließt ausdrücklich indirekte und
nachfolgende Verluste, Schäden, Kosten oder Ausgaben aus, einschließlich aber nicht beschränkt
auf Zeit-, Material- und Geldverlust oder den Verlust des Gebrauchs, von Gewinn, Produktion,
Einkommen, erwarteten Einsparungen und des Firmenwerts. Um einen Garantiefall zu melden,
wenden Sie sich bitte an den Vertriebspartner des Geräts. Diese Garantie gilt zusätzlich zu und nicht
anstelle von geltendem Recht.
Diese Anleitung wurde zur Information der Kunden von Techne erstellt und stellt in keinster Weise
eine Gewährleistung, Bedingung oder Darstellung bezüglich der Beschreibung, Marktgängigkeit oder
Zweckdienlichkeit dieser Geräte oder Bauteile dar.
Unabhängig von Beschreibung und Spezifikation(en) des hier beschriebenen Geräts behält sich
Techne das Recht vor, Änderungen an diesem Gerät oder dessen Bauteilen vorzunehmen.
10
INFORMAZIONI DI SICUREZZA
3
Leggere attentamente il presente manuale prima di usare il Prime.
AVVERTENZA
Le ALTE TEMPERATURE SONO PERICOLOSE in quanto possono provocare serie ustioni agli
operatori e dare fuoco al materiale combustibile. Gli utenti devono conoscere i seguenti pericoli
potenziali:
•
PRESTARE ATTENZIONE ED INDOSSARE GUANTI PROTETTIVI PER LE
MANI
•
NON usare sostanze combustibili vicino ad oggetti caldi
•
NON mettere in funzione lo strumento nei pressi di liquidi o gas infiammabili
•
NON collocare alcun tipo di liquido direttamente nello strumento.
SICUREZZA DELL'OPERATORE
Il personale che utilizza l’apparecchiatura Techne deve avere a disposizione la documentazione
necessaria al fine di assicurare la loro incolumità. È importante che solo personale adeguatamente
addestrato utilizzi questo apparecchio, in conformità alle istruzioni contenute in questo manuale e nel
rispetto delle normative e procedure generali di sicurezza. Se l’apparecchio è utilizzato in modo non
specificato da Bibby Scientific Ltd., la protezione fornita dall’apparecchiatura all’utilizzatore potrebbe
essere a rischio.
Tutte le unità Techne sono state progettate in conformità ai requisiti internazionali di sicurezza e sono
equipaggiate con un interruttore anti surriscaldamento. Se si dovesse verificare qualche problema di
sicurezza, disconnettere l’apparecchio dalla rete.
INSTALLAZIONE
Occorre trasportare lo strumento usando entrambe le mani. Non spostare né trasportare lo
strumento quando è in funzione o collegato all’alimentazione elettrica di rete.
1.
Tutti gli strumenti Techne sono forniti con un cavo di alimentazione; può essere integrale o
plugin.
2.
Prima di collegare lo strumento all'alimentazione elettrica di rete, controllare la
tensione confrontandola con la targhetta riportante i valori nominali (si trova sul retro
dell'unità). Notare che al fine di garantire la corretta sicurezza elettrica, occorre
che l'unità sia messa a terra. Collegare il cavo di rete ad una presa idonea secondo
la tabella riportata alla pagina successiva.
Connessione
220/240V, 50/60Hz
110V/120V, 50/60Hz
Sotto tensione
Marrone
Nero
Neutro
Blu
Bianco
Terra
Verde/giallo
Verde
3.
SOLO REGNO UNITO: la spina con fusibile fornita con il cavo di rete è dotata di un fusibile da 5
Amp per proteggere lo strumento e l'utente.
4.
Le unità sono stimate funzionare a 100-240V, 50/60Hz.
5.
Collocare l'unità su un banco piano idoneo o in una cappa aspirante se necessario, assicurandosi
che gli sfiati delle prese d'aria nella parte inferiore non siano ostruiti.
6.
Inserire il cavo di rete nella presa che si trova sul retro dello strumento.
11
7.
Accendere lo strumento per mezzo dell'interruttore situato sulla parte retro dell'unità.
Cavo di ricambio
2
Qualora occorra sostituire il cavo di rete, si dovrà utilizzare un cavo di 1mm codice armonizzato
H05VV-F collegato ad una spina IEC 320. IN CASO DI DUBBIO, RIVOLGERSI A UN
ELETTRICISTA QUALIFICATO.
CONDIZIONI AMBIENTALI
3
Il Prime è stato progettato per funzionare nelle seguenti condizioni:
•
uso interno
•
range di temperatura ambiente da +5ºC a +40ºC
•
altitudine massima 2000 m.
•
umidità relativa non superiore all’80%
•
oscillazione dell’alimentazione di rete non superiore al 10%
•
categoria di sovratensione II IEC 60364-4-443
•
grado di inquinamento 2
Nota: le specifiche di controllo sono indicate ad una temperatura ambiente di 20ºC. Le specifiche
potrebbero peggiorare fuori da una temperatura ambiente compresa tra 10ºC e 30ºC.
Lo strumento è stato collaudato per interferenze da radiofrequenze ed è certificato secondo la norma
EN61326.
GARANZIA
Bibby Scientific Ltd garantisce che, per un periodo di quattro (4) anni e se utilizzato nelle normali
condizioni di laboratorio, questo strumento non presenterà alcun difetto dei materiali o di
fabbricazione. In caso di reclamo giustificato, Bibby Scientific Ltd sostituirà qualsiasi componente
difettoso o rimpiazzerà l'unità in modo gratuito. Questa garanzia non si applica ai danni causati da
incendio, incidenti, utilizzo improprio, negligenza, regolazioni o riparazioni scorrette, danni dovuti a
installazione non corretta, adattamenti, modifiche o installazione di parti non approvate oppure
riparazioni eseguite da personale non autorizzato. La responsabilità di Bibby Scientific Ltd è limitata al
costo della riparazione o della sostituzione del prodotto ed esclude in particolare qualsiasi perdita
indiretta o secondaria, danno, costo o spesa, ivi inclusi, a titolo di esempio e in modo non esaustivo,
perdite di tempo, materiali e spese oppure perdite di utilizzo, profitto, produzione, ricavo, risparmio
atteso o avviamento. Per esporre un reclamo, contattare il fornitore dello strumento. Questa garanzia
è in aggiunta ai diritti di legge e non ha alcun effetto su di essi.
Il presente manuale è stato preparato ad uso dei clienti di Techne e niente di quanto in esso
contenuto costituisce garanzia, condizione o rappresentanza riguardo la descrizione, la
commerciabilità, l'idoneità allo scopo o altrimenti dell'unità o dei componenti.
Nonostante la descrizione e le specifiche dello strumento contenuti nel manuale dell'operatore,
Techne si riserva il diritto di apportare le modifiche ritenute opportune agli strumenti o a qualsiasi loro
componente.
12
INFORMACIÓN DE SEGURIDAD
3
Lea atentamente este manual antes de utilizar el Prime.
ADVERTENCIA
LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que pueden ocasionar quemaduras graves a
los operarios y prender el material combustible. Los usuarios deben conocer los posibles riesgos:
•
TENGA CUIDADO Y LLEVE
PROTEGERSE LAS MANOS
GUANTES
DE
PROTECCIÓN
•
NO utilice sustancias combustibles cerca de objetos calientes
•
NO utilice el instrumento cerca de líquidos o gases inflamables
•
NO coloque un líquido directamente en el instrumento.
PARA
SEGURIDAD DEL OPERARIO
Todos los usuarios de equipos Techne deben disponer de la información necesaria para asegurar su
seguridad. De acuerdo con las instrucciones contenidas en este manual y con las normas y
procedimientos generales de seguridad, es muy importante que sólo personal debidamente
capacitado opere estos aparatos. De no ser así, la protección que el equipo le proporciona al usuario
puede verse reducida.
Todos los equipos Techne han sido diseñados para cumplir con los requisitos internacionales de
seguridad y traen incorporados un sistema de desconexión en caso de sobre temperatura. En caso
de que surgiera un problema de seguridad, desconecte el equipo de la red.
INSTALACIÓN
El instrumento se debe transportar con las dos manos. No mueva ni lleve el instrumento cuando se
utilice o esté conectado al suministro eléctrico principal.
1.
Todos los instrumentos Techne se suministran con un cable de alimentación, que puede ser
integrado o ‘enchufable’.
2.
Antes de conectar el instrumento al suministro eléctrico, compruebe que el voltaje
coincida con el indicado en la placa de régimen (situada en la parte trasera de la
unidad). El instrumento debe disponer de una toma de tierra para garantizar la
seguridad eléctrica adecuada. Conecte el cable de alimentación a un enchufe
adecuado según la siguiente tabla.
Conexión
220/240V, 50/60Hz
110V/120V, 50/60Hz
Con corriente
Marrón
Negro
Neutro
Azul
Blanco
Toma de tierra
Verde/amarillo
Verde
3.
SÓLO PARA EL REINO UNIDO: El enchufe suministrado con el cable de alimentación incluye
un fusible de 5 amperios para ofrecer protección al instrumento y al usuario.
4.
Las unidades se clasifican para funcionar en 100-240V, 50/60Hz.
5.
Sitúe la unidad sobre una mesa plana o en una campana de laboratorio si es necesario, y
asegúrese de que los orificios de ventilación situados en la parte inferior no tienen ninguna
obstrucción.
6.
Conecte el cable de alimentación en el enchufe situado en la parte trasera del instrumento.
13
7.
Encienda el instrumento usando el interruptor situado en la parte posterior de la unidad.
Cable de repuesto
2
Si es necesario sustituir el cable de alimentación, se debe utilizar un cable de 1mm de código
armonizado H05VV, conectado a un enchufe IEC320. EN CASO DE DUDA, PÓNGASE EN
CONTACTO CON UN ELECTRICISTA.
CONDICIONES AMBIENTALES
3
El Prime está diseñado para utilizarse en las condiciones siguientes:
•
Uso en interior
•
Intervalo de temperatura ambiente +5ºC a +40ºC
•
Altitud: hasta 2000 m
•
Humedad relativa no superior al 80%
•
Fluctuaciones del suministro eléctrico no superiores al 10%
•
Categoría de sobrevoltaje II IEC 60364-4-443
•
Nivel de contaminación 2
Nota: Las especificaciones de control corresponden a una temperatura ambiental de 20ºC. Las
especificaciones pueden empeorar si se utiliza el instrumento fuera del intervalo de temperatura
comprendido entre 10ºC y 30ºC.
Se han realizado pruebas para comprobar la interferencia de radiofrecuencia del instrumento, el cual
cumple la normativa EN61326.
GARANTÍA
Bibby Scientific Ltd garantiza que este equipo estará libre de defectos de materiales y mano de obra,
si se utiliza en condiciones normales de laboratorio, durante un período de cuatro (4) años. En caso
de reclamación justificada, Bibby Scientific Ltd sustituirá cualquier componente defectuoso o
cambiará la unidad gratuitamente. Esta garantía no es aplicable si los daños han sido ocasionados
por incendios, accidentes, usos inapropiados, negligencias, ajustes o reparaciones incorrectas, así
como si se trata de daños ocasionados por la instalación, adaptación, modificación o incorporación
en el instrumento de piezas no aprobadas o su reparación por personal no autorizado. La
responsabilidad de Bibby Scientific Ltd se limita al coste de la reparación o sustitución del producto,
y excluye específicamente pérdidas, daños, costes o gastos indirectos o accidentales, incluyendo
pero sin estar limitado a ello, pérdidas de tiempo, materiales y gastos o pérdida de uso, beneficios,
producción, ingresos, ahorros previstos u oportunidades comerciales. Si desea realizar una
reclamación, póngase en contacto con el suministrador del equipo. Esta garantía complementa y no
afecta a sus derechos legales.
Este manual se ha preparado con una finalidad informativa para los clientes de Techne y ninguna
parte del manual se deberá considerar como una garantía, condición o reflejo con respecto a la
descripción, comerciabilidad, idoneidad para un fin determinado o de otro tipo de la unidad o sus
componentes.
Con independencia de la descripción y las especificaciones del instrumento que se indican en el
manual del operario, Techne se reserva el derecho de realizar cambios en el instrumento o en
cualquiera de sus componentes cuando lo estime oportuno.
14
CONTACT INFORMATION
For technical, sales or servicing information, contact your local Techne dealer or:
UK
Bibby Scientific Ltd.
Beacon Road
Stone
Staffordshire
ST15 0SA
UK
Tel: +44 (0)1785 812121
Fax: +44 (0)1785 810405
E-mail: [email protected]
www.techne.com
North and South America
Bibby Scientific US Inc. t/a Techne Inc.
3 Terri Lane, Suite 10
Burlington, N.J. 08016
USA
Toll Free (in NA): 800-225-9243
Tel: +1 609 589 2560
Fax: +1 609 589 2571
E-mail: [email protected]
www.techneusa.com
France
Bibby Scientific Limited
Bâtiment Le Deltaparc Parc Silic PN2
7 rue du Canal
BP 55437 VILLEPINTE
95944 ROISSY Charles de Gaulle
France
Tel: +33(0)148 63 78 03
Fax: +33(0)148 63 78 01
E-mail: [email protected]
www.bibby-scientific.com
Italy
Bibby Scientific Italia Srl
Via Alcide de Gasperi 56
20070 Riozzo di Cerro al Lambro (MI)
Italia
Tel: +39 (0)2 98230679
Fax: +39 (0)2 98230211
E-mail: [email protected]
www.bibby-scientific.it
Middle East
Bibby Scientific Middle East Ltd.
PO Box 27887, Engomi 2433
Nicosia
Cyprus
Tel: +357 22 660 423
Fax: +357 22 660 424
e-mail: [email protected]
Asia
Bibby Scientific - Singapore
Prudential Tower, Level 26
30 Cecil Street
Singapore 049712
Tel: +65 6631 2976
Fax: +44 (0) 1785 810405
e-mail: [email protected]
www.bibby-scientific.com
15
3
PRIME SPECIFICATION
3
Sample capacity: 0.2ml
Sample capacity: 0.5ml
Block temperature range
Block uniformity (at 50ºC)
Temperature accuracy (at 50ºC)
Maximum heating rate
Maximum cooling rate
Gradient
Gradient availability
Maximum gradient range
Minimum gradient range
Type
Pre-run sample cooling
Temperature resolution
Selectable heated lid temperature
Heated lid pressure
Pre-heat lid
Program interface
Maximum number of programs
Maximum number of stages per
program
Maximum number of steps per
stage
Maximum number of cycles per
stage
Programmable ramp rate
Maximum hold time
Minimum hold time
Gradient calculator
Oligonucleotide Tm calculator
Incremented/decremented
temperature
Incremented/decremented time
Pause facility
Program naming
Password protection
Run completion time
Auto resume on power failure
Data transfer
Software updates
Dimensions (L x W x H)
Weight
Voltage
Power
Prime
3
PrimeX
3
PrimeG
48
48
30
30
4ºC (10°C during cycling) to 100ºC
<±0.3ºC
<±0.2ºC
3.0ºC/s
2.1ºC/s
Can be enabled
Yes
30ºC to 80ºC
14ºC
1ºC
2 x Peltier
4 x Peltier
4 x Peltier
element/block
element/block
element/block
Yes, 4ºC
0.1ºC
35ºC to 115ºC or off
Adjustable depending on
Fixed
consumable
Yes
3.5” colour VGA LCD touch screen
1000
24
18
10
10
99
Yes, 0.1ºC/s steps
4h 59m 59s
1s
Yes
1
Yes, based on the Nearest-Neighbour method
Yes
Yes
Yes
Alpha numeric plus symbols
Yes
Yes
Yes, always or never
USB port
Free of charge from www.techne.com
350 x 210 x 180mm
6kg
100-230V, 50/60Hz
155W
1
Breslauer, K.J.; Frank, R.; Blocker, H. and Marky, L.A. (1986) Proc. Natl. Acad. Sci. USA 83, pp
3746-3750.
16
INSTALLATION
See also the Safety Information sections on pages 5 to 14.
Front view
1.
2.
3.
4.
Rear view
3
Lid adjustment knob ( PrimeX and G only).
Lid release latch.
3.5” colour VGA LCD touch screen display.
USB port for memory stick.
1.
2.
3.
Installation
1.
Place the unit on a suitable flat bench, ensuring that
the air inlet vents on the sides are free from
obstruction.
2.
Plug the mains cable into the socket on the back of
the instrument.
3.
Connect to the mains electricity supply with the plug
provided or one wired correctly for the supply. Switch
the power ON using the switch located on the rear of
the unit. The front display will then light up.
4.
Release the heated lid by lifting the latch forward and
use it to lift the lid. To close the lid, lower the lid onto
the block and press the latch to secure in place.
5.
Place the samples in the block. If individual tubes or
strip tubes are being used, space these out evenly
across the block to equalise the pressure from the
heated lid.
17
USB port for service and calibration only.
On/Off rocker switch.
Mains cable inlet.
3
6.
7.
With the PrimeX and G, the heated lid has a
rotating knob to adjust the lid pressure on the
samples, allowing for a variety of consumables to
be used. To adjust the pressure:
a.
Place the samples in the block and place the lid
in the down position.
b.
Adjust the orange rotating knob anticlockwise
to give the least pressure then close the lid
fully.
c.
Gently rotate the knob clockwise until you can
feel pressure beginning to be applied.
d.
Rotate the knob a further quarter of a turn and
this will give the correct pressure. Do not overtighten.
Once the pressure has been set it does not need to be adjusted unless a different block or
consumable is used.
18
GENERAL SOFTWARE FEATURES
INTRODUCTION
3
The Prime software allows the user to quickly and easily create thermal cycling programs using a
simple and clear touch screen format. The software is structured into three modules which are
accessed from the Home Screen. These allow the user to:
• Run an existing program, selecting from templates or
programs saved on the unit.
• Create a new program.
• Set the instrument and program defaults and
preferences. Includes calculator tools.
TEMPLATE PROGRAMS
To help with programming, a number of program templates are installed on the unit which are
available to copy and edit or can be run directly without changes. Details of the installed program
templates are given below.
Instrument and program defaults
Parameter
Heated Lid
Heated lid before program
Sample cooling
Pause before program
Initial denaturation
Hot start
Final extension
Final hold
2 Step Template
105ºC
On
On
Off
94ºC, 05m00s
Off
72ºC, 05m00s
10ºC
3 Step Template
105ºC
On
On
Off
94ºC, 05m00s
Off
72ºC, 05m00s
10ºC
RT-PCR Template
105ºC
On
On
Off
94ºC, 05m00s
Off
72ºC, 07m00s
10ºC
Thermal cycling conditions
Stage 1
Number of cycles
Step 1
Step 2
Step 3
Stage 2
Number of cycles
Step 1
Step 2
Step 3
2 Step Template
3 Step Template
RT-PCR Template
30
94.0ºC, 00m30s
60.0ºC, 01m00s
30
94.0ºC, 00m30s
55.0ºC, 00m30s
72.0ºC, 00m30s
1
45.0ºC, 40m00s
94.0ºC, 05m00s
40
94.0ºC, 01m00s
55.0ºC, 00m50s
72.0ºC, 01m00s
The Ice Bucket and Ligation programs are simple temperature holds, with the former having an infinite
hold at 10ºC and the latter at 15ºC. In both of these programs the heated lid is switched off and the
initial denaturation, hot start and final extension functions disabled.
19
INCREMENTED/DECREMENTED TIME AND TEMPERATURE
The software allows the user to either increment or decrement the time and/or temperature of a step
within a cycling stage. These features are used with applications such as touchdown PCR where the
annealing temperature is gradually decreased during the cycling process and long PCR, where due to
the size of the product, long annealing/extension times are required and the extension time is
increased by 15 to 20s per cycle during the final stages of the reaction.
GRADIENT (3PRIMEG ONLY)
3
The gradient feature of the PrimeG can be useful in optimising the annealing conditions for primers. A
gradient can be set around a temperature in any step of a program. The set temperature is the
temperature in the middle columns of the block and the gradient is the variation at the two extremes of
the block; the left hand columns being the coolest and the right hand columns the hottest.
The maximum temperature gradient range which can be set is 14ºC and the minimum is 1ºC; the
lowest temperature is 30ºC and the hottest temperature is 80ºC. Examples showing approximate
temperatures are given in the table below:
Lowest temperature
(ºC)
50
30
66
Set temperature
(ºC)
55
37
73
Highest temperature
(ºC)
60
44
80
Gradient
(ºC)
10
14
14
A gradient calculator is included in the Settings module so the user can obtain an accurate estimation
of the temperature of each column during the gradient stage. The temperatures for any particular
gradient are calculated from an algorithm stored in the block firmware.
OLIGONUCLEOTIDE TM CALCULATOR
One of the most important parameters in a PCR is the annealing temperature of the reaction. This can
be determined theoretically using various software programmes to calculate the melting temperature
(Tm) of the primers.
20
The Tm is defined as the temperature at which half the DNA strands are single stranded and half are
double stranded when base paired to a complimentary strand. Using annealing temperatures that are
well below the Tm of the primers can result in mismatching, false priming and may lead to primer-dimer
artefacts. Annealing temperatures that are significantly higher than the primer Tm may result in
reduced priming or prevent priming altogether.
3
1
The Prime uses the Nearest-Neighbour method for calculating the Tm of an oligonucleotide .
The Tm is calculated using the following equation:
+
Tm
=
{(∆Hº x 1000)/(A + ∆Sº + R ln(C/4))} - 273.15 + 16.6 x log[Na ]
∆Hº
A
∆Sº
R
C
[Na+]
=
=
=
=
=
=
The sum of the Nearest-Neighbour enthalpy changes (Kcal/mol)
Helix initiation correction constant
-1
-1
The sum of the Nearest-Neighbour entropy changes (cal K mol )
-1
-1
The Gas Constant (1.99 cal K mol )
Concentration of the oligo (M)
Concentration of monovalent ions (M)
Where:
1
Breslauer, K.J.; Frank, R.; Blocker, H. and Marky, L.A. (1986) Proc. Natl. Acad. Sci. USA 83, pp
3746-3750.
The Oligonucleotide Tm calculator can be found in the Settings module where the user can input the
oligonucleotide sequence, its concentration in the reaction and the salt concentration of the reaction
mix.
21
PROGRAMMING TEXT, TEMPERATURE AND TIME
This section gives instructions for the basic programming of text, temperature and time which are
required in later sections.
TEXT ENTRY
When giving a name to a program or stage a text entry screen will be opened.
• Touch a character/numerical button repeatedly to
cycle through the characters. Touch and hold for
numerals.
• Touch shift for upper case characters.
• Touch the 1/space bar button to enter spaces
between characters.
• To delete the last character touch the left arrow.
• Touch More for commonly used symbols.
• Touch More again to return to characters and
numerals.
Once the name is complete, touch Back.
TEMPERATURE ENTRY
When touching a temperature parameter a temperature entry screen will open. There are two forms of
temperature entry screen:
• A simple form for adjusting the temperature of the instrument parameters such as the heated lid
and program parameters such as initial denaturation and final extension.
• An advanced form when programming step temperatures within a cycling stage that includes the
options for introducing an incremented/decremented step temperature, for example in touchdown
3
PCR, or a temperature gradient if using the PrimeG.
22
Simple temperature entry
• If necessary, first touch the button next to the
parameter (e.g. Heated Lid) to turn it ON.
• Next touch the Temp button.
A screen similar to that shown opposite is opened. The
maximum and minimum allowed values are indicated
depending on the parameter being set.
• Touch the white temperature area to open the
temperature entry screen.
• Enter the temperature using the numerical keys. The
resolution is one decimal place.
• Use the arrow key to delete the last character.
• Once the temperature is correct, touch Back to
return to the previous screen.
This screen will now display the set temperature.
• Touch OK to return to the parameter screen.
• Touch Cancel to return to the parameter screen if no
changes are required.
23
Advanced temperature entry
• First ensure the number of cycles for the stage has been set then enter the required hold
temperature as described above.
Note: Increments and Decrements will be inactive unless the number of cycles set is greater than 1.
To increase or decrease the step temperature per
cycle:
• Touch the step Temp button. This will open the
temperature screen as shown opposite.
• Touch Increments and Decrements.
3
The increment, decrement and gradient ( PrimeG only)
screen will open.
• Touch the button next to the required parameter to
turn it ON.
• Next touch the temperature button to set the
increment or decrement per cycle.
A new screen will open which is similar to the simple
temperature entry screen.
• Enter the required value followed by Back.
• If an entered temperature value is invalid, a prompt
will indicate the acceptable range.
• Touch Accept to return to the previous screen.
• Touch OK to accept the modification and return to
the program.
• Touch Cancel to return to the programming screen if
no changes are required.
24
The temperature value will now appear orange in the
programming screen indicating that it contains a
modified function.
Note: It is not possible to both increase and decrease
the temperature of the same step.
3
To enter a temperature gradient on a step ( PrimeG
only):
3
On the PrimeG an additional Temperature Gradient
button is available.
• Touch the step Temp button to open the temperature
screen.
• Touch Increments and Decrements.
The increment, decrement and gradient screen will
open.
• Touch the button next to the gradient parameter to
turn it ON.
• Next touch the temperature button to set the gradient
range.
A new screen will open which is similar to the simple
temperature entry screen.
• Enter the required value followed by Back.
• If an entered temperature value is invalid, a prompt
will indicate the acceptable range.
• Touch Accept to return to the previous screen.
• Touch OK to accept the modification and return to
the program.
• Touch Cancel to return to the programming screen if
no changes are required.
25
The temperature value will now appear orange in the
programming screen indicating that it contains a
modified function.
Note: It is not possible to have both a gradient and an
increment or decrement temperature in the same step.
TIME ENTRY
When touching a time parameter a time entry screen will open. There are two forms of time entry
screen:
• A simple form for adjusting the time of the program parameters such as initial denaturation and
final extension.
• An advanced form when programming step hold times within a cycling stage that includes the
options for introducing incremented/decremented time, for example in long PCR.
Simple time entry
• If necessary, first touch the button next to the
parameter (e.g. Initial denaturation) to turn it ON.
• Next touch the Time button.
A screen similar to that shown opposite is opened. The
maximum and minimum allowed values are indicated
depending on the parameter being set.
• Touch the white time area to open the time entry
screen.
26
• Enter the time using the up and down arrow keys (h,
m, s).
• Once the time is correct, touch OK to return to the
previous screen.
This screen will now display the set time.
• Touch OK to return to the parameter screen.
• Touch Cancel to return to the parameter screen if no
changes are required.
Advanced time entry
• First ensure the number of cycles for the stage has been set then enter the required hold time as
described above.
Note: Increments and Decrements will be inactive unless the number of cycles set is greater than 1.
To increase or decrease the step hold time per
cycle:
• Touch the step Time button. This will open the time
screen as shown opposite.
• Touch Increments and Decrements.
The increment and decrement time screen will open.
• Touch the button next to the required parameter to
turn it ON.
• Next touch the time button to set the increment or
decrement per cycle.
27
A new screen will open which is similar to the simple
time entry screen.
• Enter the required value (h, m, s) followed by OK.
• If an entered time value is invalid, a prompt will
indicate the acceptable range.
• Touch Accept to return to the previous screen.
• Touch OK to accept the modification and return to
the program.
• Touch Cancel to return to the programming screen if
no changes are required.
The time value will now appear orange in the
programming screen indicating that it contains a
modified function.
Note: It is not possible to both increase and decrease
the time of the same step.
28
INSTRUMENT SETTINGS AND DEFAULTS
Touch the Settings button to access the instrument Settings menu.
• Touch any of the buttons to view and edit the
settings.
• Touch More Settings to access further options.
• Once all the settings and defaults have been set as
required, touch Save to save the changes and
return to the Home Screen.
• If you do not wish to change any settings, touch
Cancel to return to the Home Screen.
USER PREFERENCES
Language
• Touch the language button to set the required
language. The options are English, French, Spanish,
Italian and German.
Temperature Display
• Touch the temperature button to set the required
temperature units. The options are ºC and ºF.
• When set, touch Back to return to the Settings
menu.
INSTRUMENT SETTINGS
Auto Resume On Power Loss
This determines whether the unit will restart after a
power failure. Setting Auto Resume to Always will
always allow the unit to re-start irrespective of the
length of the power failure.
29
If Auto Resume is set to Always and the power is
interrupted, on return of the power supply, a message
will be displayed on the screen giving details of when
the power was lost, when it was restored and the name
of the program running at the time.
• Touch OK to return to the Run Screen.
Date and Time Settings
Use these settings to set the current date, time and
date format on the unit.
• When set, touch Back to return to the Settings
menu.
Date
• Touch Set Date followed by the white date button in
order to enter the current date.
• Use the up and down keys to set the date values
(dd/mm/yy).
• When the correct date has been set, touch OK to
return to the previous screen.
• Touch Cancel to return to the previous screen if no
changes are required.
Time
• Touch Set Time followed by the white time button in
order to enter the current time using the 24 hour
clock.
• Use the up and down keys to set the time values (h,
m, s).
• When the correct time has been set, touch OK to
return to the previous screen.
• Touch Cancel to return to the previous screen if no
changes are required.
Date Format
Touch the date button to set the required date format.
The options are dd/mm/yy or mm/dd/yy.
30
• Touch Back to return to the Instrument Settings
main menu.
• Touch More Settings for further Instrument
Settings.
Administrator Settings
This allows an administrator to remove passwords from
password-protected programs, change the
Administrator password and restore the unit to its
factory settings.
• Touch None and enter the administrator password
followed by Back.
The default password is 1234.
• Touch OK to enter the Administrator Settings menu.
• Touch Cancel to return to the menu without making
any changes.
Remove Passwords
• From the Administrator menu touch Remove
Passwords.
• Touch the file with the password to be removed.
• Touch Remove Password.
The password will now be removed and the program
can be deleted if required.
• De-select the program then touch Close to return to
the Administrator menu.
Change Administrator Password
This allows the administrator to change the default
password (1234).
• From the Administrator menu touch Change
Administrator Password.
• Touch the upper None button and enter the new
password.
• Touch the lower None button to confirm the new
password.
31
Note: It is recommended that the administrator keeps a
record of the new password.
• Touch OK to return to the Administrator menu or
Cancel to return without making any changes.
Restore Factory Settings
All changes to the instrument defaults and settings can
be removed and the unit returned to its factory settings.
• From the Administrator menu touch Restore
Factory Settings.
• Touch the None button and enter the administrator
password followed by Back.
• Touch Restore Settings and the unit will be reset to
factory defaults.
• Touch Cancel to return to the Administrator menu
without making any changes.
• Touch Back to return to the Settings menu.
PROGRAM DEFAULTS
3
The program default settings will apply to every new program created on the Prime. However
individual programs can be edited as required.
Heated Lid
Use this to set the heated lid temperature to
approximately 10ºC higher than the hottest block
temperature in the program.
• Touch On/Off to toggle between settings.
• Touch the temperature button to set the required lid
temperature (35 to 115ºC).
Preheat lid
Set to On to pre-heat the lid before the thermal cycling
program begins. The lid will pre-heat for 2 minutes
before the program starts.
• Touch On/Off to toggle between settings.
Sample Cooling
Set to On to cool the block to 4ºC while the lid preheats.
• Touch On/Off to toggle between settings.
• Touch More Settings for further program defaults or
Back to return to the Settings menu.
32
Pause
This will cause the unit to pause before the program
starts at the ambient temperature of the block (unless
Sample Cooling has been set to On when the unit will
pause with the block held at 4ºC). The program will not
commence until the unit is manually un-paused.
• Touch On/Off to toggle between settings.
Initial Denaturation
Use this to denature the sample and/or activate the
enzyme before the thermal cycling program begins.
• Touch On/Off to toggle between settings.
• Touch the time button to set the required hold time.
• Touch the temperature button to set the required
temperature.
Hot Start
This will cause the unit to pause at the set temperature.
The program will not continue until the unit is manually
un-paused.
• Touch On/Off to toggle between settings.
• Touch the temperature button to set the required
temperature.
• Touch More Settings for further program defaults or
Back to return to the Settings menu.
Final Extension
Use this to add a final extension step to the program to
ensure complete amplification of the products.
• Touch On/Off to toggle between settings.
• Touch the time button to set the required hold time.
• Touch the temperature button to set the required
temperature.
Final Hold
This will hold the block at the set temperature at the
end of a program until the user manually stops the unit.
• Touch On/Off to toggle between settings.
• Touch the temperature button to set the required
temperature.
• Touch More Settings to return to the top of the
Program Defaults menu or Back to return to the
Settings menu.
33
SERVICE SETTINGS
The service settings are for the use of authorised
service engineers only. A service engineer’s password
is required to access these settings.
GRADIENT CALCULATOR (3PRIMEG ONLY)
The Gradient Calculator can be used to calculate the temperature for each column of the block for a
user-specified temperature and gradient range.
• Touch the Target Temperature button and enter
the required temperature.
• Touch OK.
• Touch the Temperature Gradient button and enter
the required gradient range followed by OK.
• Touch OK.
The calculated temperatures for each of the columns of
the block will now be displayed.
• Touch Back to return to the main menu
OLIGO TM CALCULATOR
The oligo Tm calculator can be used to estimate the Tm of the PCR primers and help in selecting an
appropriate annealing temperature for the thermal cycling program.
• Touch Enter The Oligo Sequence in order to input
the sequence of the primer.
• Touch the white DNA Base Entry button.
34
• Touch the A C G T buttons to enter the primer
sequence.
• Touch the arrow to delete the last character.
• Use the < and > keys to scroll through the
sequence.
• Touch Back to return to the previous screen
followed by OK.
• Enter the final primer concentration in the PCR mix.
• Enter the final monovalent salt concentration of the
PCR mix.
• Touch Calculate to calculate the Tm of the primer.
• To check the Tm of another primer with the same
primer and salt concentrations, touch Back and
enter the new primer sequence.
• Touch Reset to clear the entries.
• Touch Back to return to the Settings menu.
• Once all the settings and defaults have been set as required, touch Save to save the changes and
return to the Home Screen.
• If you do not wish to change any settings, touch Cancel to return to the Home Screen.
35
CREATE A NEW PROGRAM
From the Home Screen, touch the New Program
button. This will open a screen where you can build
a program by adding stages and steps and define
the temperatures and hold times.
INSERT/DELETE A STAGE
A stage is a section of a program that consists of a number of individual steps that can be cycled
(repeated) a defined number of times.
Insert a new stage
• To insert a stage in a program, touch Insert a
new stage. A stage with one step will be
inserted.
Stage 1
• Touch this to give the stage a name (optional).
Enter the name using the text entry screen.
1 cycle
• Touch this to edit the number of cycles
(repeats) for the stage. Enter the required
number of cycles then touch Back.
Temp
• Touch the temperature button to edit the
temperature of the step and add any
increment/decrement or gradient features.
Note: The minimum step temperature that can be
programmed during cycling stages is 10°C. If the
step temperature is above or below the valid
3
gradient range ( PrimeG only) then the gradient
function is disabled.
Time
• Touch the time button to edit the hold time of
the step and to add any increment/decrement
time features.
36
Ramp rate
• Touch the ramp rate setting to adjust the ramp
rate.
The range is 0.1ºC/s (minimum) to 3.0ºC/s
maximum for heating and 2.1ºC/s for cooling. The
value can be entered to 1 decimal place. Ramp
rate will affect the heating or cooling rate from the
previous step to the current step.
• Once set, touch OK to return to the
Programming Screen.
Delete a stage
• To delete a stage, touch the stage area to
highlight it and then touch Delete.
INSERT/DELETE A STEP
Insert a new step
• Touch Insert a new step to insert the next step
in the current stage.
• Edit the new step temperature, hold time and
ramp rate as required.
Delete a step
• To delete a step, touch the area near to the
step number to highlight it and then touch
Delete. More than one step can be selected at
a time.
INSERT/DELETE A PAUSE
Insert a pause
• To insert a pause between stages, touch Insert
a pause.
The hold temperature will be that of the previous
step. The unit will not continue with the remainder
of the program until it is manually un-paused by the
user.
Delete a pause
• To delete a pause, touch the pause button to
highlight it and then touch Delete.
Note: To insert a pause between two previously
programmed stages, it is necessary to first delete
the later stages to where the pause is required.
Continue adding stages and steps as required. Use the up and down arrow buttons to scroll through
the program as necessary.
37
THE MORE BUTTON
The More button can be used to access the
Program Options and Instrument Settings
defaults for editing in the current program.
Program Options
• Touch this to view the program defaults as set
up in the Settings module. Use this also to
name the program and add a password to
protect it from editing by other users.
Settings
• Touch this button to view the instrument
defaults as set up in the instrument Settings
module.
Save
• Touch this to save the program.
Cancel
• Touch this to cancel changes to the program
and return to the Home Screen.
PROGRAM OPTIONS
Program Name
• Touch Name the program to give the program
a name.
• Type in the required name using the text entry
screen.
• Once the name is complete, touch Back to
return to the Program Options screen.
• Touch Back to return to the Program Options
and Settings screen.
• Touch Back again to return to the programming
screen.
When saving the program, if the chosen name has
already been used for another program, the
message box shown opposite will appear.
• Touch OK to return to the Program Options and
Settings screen.
• Open Program Options, touch the program
name and enter a different name.
• Once the name is complete, touch Back twice
to return to the programming screen.
38
Initial denaturation, Final Extension and Final
Hold
• Touch the time and temperature buttons to edit
these if required.
• Use the Down arrow to scroll to further options.
Pause and Hot Start
• Touch On/Off to toggle between settings.
• Set the temperature for Hot Start if required.
Password
A password can be created to protect the program
from being edited and deleted by other users. The
password will be requested if a user attempts to
edit or delete the program.
• To create a password, touch the None button
next to Password.
• Enter the required password then touch Back to
return to the Program Options.
Note: The password is case-sensitive.
• To change or remove the password, from the
Program Options screen, touch the button next
to Password.
• On the Password Editing screen, touch the
None button and enter the password.
• To remove the password, touch Remove
Password.
• To change the password, touch Change
Password and type in the new password,
followed by Back.
• Touch Cancel to return to the Program Options
screen if no change is required.
39
SETTINGS
• Touch the Settings button to view the
instrument defaults as set up in the instrument
Settings module.
Any of these parameters can be edited by touching
the appropriate button and will be saved for the
current program only.
• Touch Back twice to return to the programming
screen.
40
RUN A PROGRAM
From the Home Screen, touch the Run button. This will
open a screen where you can select a program to run,
edit, copy or delete.
• Touch a program to select it. Use the arrow buttons
to scroll down the list of stored programs.
Once a program is selected, a series of options are
displayed across the bottom of the screen:
• Touch Run to send the program to the unit and
proceed to the pre-run screen.
• Touch Edit to open the program and allow editing.
• Touch Copy to make a copy of the program before
editing.
• Touch Delete to delete the selected program.
• Touch Search to search for a program in the list.
Each of these is discussed in more detail below.
To return to the Home Screen, de-select the program
and touch Close.
41
RUN
3
Touching the Run button sends the selected program to the Prime, ready to start the run.
The Pre-run screen
This screen shows a step-by step representation of the
program in a tile format. The program name is given at
the top of the screen followed by the stage name.
The first step tile is highlighted and enlarged in the
centre of the screen.
• Touch the arrows to scroll forwards and backwards
through the program, or drag a finger across the
screen.
The step tiles show the set incubation temperature. The
white bar underneath shows the stage number and
number of cycles within the stage.
The bottom of the screen shows the expected end time
if the program was started immediately.
• Touch Start to start the program.
• Touch Edit to edit the program.
• Touch Back to return to the previous screen.
The Run Screen
Once the program has been started, the current step is
shown by the central highlighted step tile. The tile shows
the set temperature, the incubation time and the status
i.e. ramping to temperature, paused or the count down
time of the step.
The white bar underneath shows the current cycle
number within the stage.
Timers at the bottom of the screen indicate how long the
program has been running and give an estimate of the
total run time to completion. Total run time is
recalculated throughout the run.
• Touch Stop to stop the program. A prompt will ask
you to confirm the stop. Touch Yes to confirm or
Cancel to continue with the run.
• Touch Pause to pause the program. If paused, touch
Continue to un-pause the program.
• At the end of a run, if the program is in a final hold,
touch Finish to end the program.
42
• Touch Temperature log to view the block
temperatures. The previous two minutes of
temperature readings can be viewed by scrolling
across the screen using a finger.
Note: Touch Program View to return to the program
status.
EDIT
Editing existing programs
• First select the required program and touch Edit.
Any of the Program Options, Settings or thermal cycling
parameters can be edited.
• Once all the required changes have been made,
touch Save.
Note: this will overwrite the existing program.
Editing templates
Editing templates can be a quick method of creating a
program by just changing a few key parameters.
However it is not possible to save changes to the
templates installed on the thermal cycler.
If the edited program is a template, touching Save will
open the message box shown opposite.
• Touch the None button next to New Program
Name and enter a name for the edited program.
• Touch Save As New Program to save the changes.
The re-named program will now be listed in the program
list.
43
Editing password protected programs
If the edited program is password protected, touching
Save will open the message box shown opposite. To
save the changes either:
• Touch the None button next to Password and enter
the correct password followed by Save.
Note: this will overwrite the existing program.
or
• Touch the None button next to New Program
Name and enter a new name for the edited
program, followed by Save as a New Program.
The re-named program will now be listed as a separate
program in the program list.
COPY
Program files can either be copied for editing, or to and
from the unit using a USB memory stick.
To copy a program file to the unit:
• Select a file to copy by touching to highlight it.
• Touch Copy.
A message box will confirm that the file has been
copied.
The copied file will now be highlighted and listed as
Copy of… and can be edited as required.
44
To copy a program file to or from a USB memory
stick:
• Insert a USB memory stick into the port on the front
of the unit.
A message screen will appear confirming that the USB
memory stick has been inserted.
• Touch Yes to view the files on the USB memory stick
or touch Cancel to view the files saved on the
3
Prime.
• Select a file to copy by touching the appropriate file
button to highlight it.
• Touch Copy.
A message screen will appear giving the option of
saving the file to the unit or to the USB memory stick.
• Touch the upper button to save the file to the USB
memory stick.
• Touch the lower button to save a copy of the file on
3
the Prime unit.
• Touch Cancel to return to the program list screen.
The file will be saved on the USB memory stick in a
folder called FileSystem as a .cyclerprogram file and
can be opened in the Techne Workbench PC software.
DELETE
Unused or old files can be permanently deleted from the
unit or a USB memory stick.
• Select the file to delete by touching the appropriate
file button.
• Touch Delete. A message box will appear asking for
confirmation of delete.
• Touch Yes to confirm the delete.
• Touch Cancel to abort the delete.
If the delete is confirmed, a message box will confirm
that the files have been deleted.
• Touch OK.
45
SEARCH
Programs saved on the unit can be searched by
program name.
• Touch Search followed by the button on the Search
screen.
• Enter a search term using the Text Entry screen. The
first few characters of the program name are
sufficient.
• Touch Back to return to the previous screen followed
by Search.
All programs containing the search term will be listed.
• Touch Clear Search to remove the search term.
46
UPDATING THE 3PRIME SOFTWARE
3
The Prime software can be updated by downloading the latest version from the Techne website
www.techne.com. You will need a USB memory stick on which to save the update.
Note that the update tool requires at least Windows XP SP2 and the Microsoft .Net Framework 3.5
SP1, which, if needed, can be downloaded from:
http://www.microsoft.com/downloads/details.aspx?familyid=AB99342F-5D1A-413D-831981DA479AB0D7&displaylang=en
• From the website, click on Support followed by
Software Downloads.
3
• Select the Prime Update and choose the option to
run the file.
• Insert a USB memory stick into the PC and click to
select.
• Click on Prepare the USB memory stick.
This will copy the update to the USB memory stick.
3
• Turn the Prime off and insert the USB stick into the
front USB port.
3
• Turn the Prime on and follow the instructions on the
screen.
47
UPGRADING FROM 3PRIMEX TO 3PRIMEG
3
3
The PrimeX thermal cycler can be easily and quickly upgraded to the gradient PrimeG version by
purchasing the 3PRIMEX/USB upgrade. The upgrade consists of a license certificate printed with a
unique upgrade code which allows gradient functionality to be installed on any non-gradient Techne
3
PrimeX thermal cycler.
3
Purchase of this license certificate authorises the owner of any non-gradient Techne PrimeX thermal
cycler to lawfully utilise the gradient capability of that thermal cycler in all European and American
geographies. The license is machine-specific and non-transferable.
Gradient upgrade procedure:
3
•
Turn on the PrimeX unit.
•
On the home screen, touch Settings and then More
Settings, followed by Add Gradient Feature.
•
Go to the address www.techne.com. Click on
Support then Technical Support. Under Further
Information click on Thermal Cycler Gradient
Upgrade.
•
Insert the serial number from the PrimeX into the
website twice as instructed.
•
Insert the upgrade code printed on the certificate into
the website.
•
Click Show confirmation number.
•
Insert the upgrade code and confirmation number
3
into the PrimeX and touch Validate.
•
The PrimeX is now upgraded.
3
3
48
FAQS
Q1
A1
How do I adjust the pressure of the heated lid for my tubes or plates?
For thermal cyclers with lid adjustment knobs, rotate the knob anticlockwise until there is
no pressure on the consumable, then close the lid and latch it. To obtain the correct
pressure gently rotate the knob clockwise until it is possible to just feel the pressure being
applied. Finally, rotate the knob a further quarter of a turn; the lid is now at the correct
pressure for use with the consumable. Ensure that all consumables used in the block are
of the same height and are spread evenly across the block. Insert empty “dummy” tubes if
necessary to spread the pressure of the heated lid evenly.
Q2
A2
What is the '”Pause” function at the start of the program used for?
Some users prefer to preheat the heated lid before placing the samples into the unit. The
pause feature is used to pause the unit after the 2 minute heated lid preheat step.
Q3
A3
What is “Sample Cooling”?
Sample cooling can be applied during the lid pre-heat phase. This maintains the block at
4ºC while the lid is heating to temperature and avoids any increase in sample temperature
due to the heated lid before the thermal cycling program begins.
Q4
A4
What is "Hot start"?
The Hot Start step is used to pause the machine at a specific temperature, typically
around 70°C, after the initial template denaturation. The reason is to allow the manual
addition of unmodified Taq DNA polymerase which may lose activity if added during the
initial denaturation step. Heat-activated Taq or Hot Start enzymes do not require this step.
Q5
A5
What is the incremented time and temperature function for?
Incremented/decremented time and temperature are used to increase or decrease either
the time or temperature incrementally over the number of cycles in a stage.
Incrementation of extension time is used with 'Long PCR' which is when large template
fragments are to be amplified (e.g. 27kb lambda DNA, 40kb genomic DNA). Decremented
temperature is used for protocols such as 'Touchdown PCR' where the first cycle starts
with a high annealing temperature and over the number of cycles in the stage there is a
gradual decrease in the temperature. This ensures that only the specific product is
amplified.
Q6
A6
What is a gradient thermal cycler?
Gradient blocks enable a particular temperature step in a protocol to be programmed so
that the temperature varies across the block. By specifying a temperature and the gradient
to be applied, each column will achieve a different temperature. The set temperature is in
the middle of the block, the lowest on the left and the highest on the right. The gradient is
the total difference across the block, for example if the set temperature was 60°C and the
gradient 10°C then the temperatures would range from approximately 55°C to 65°C from
left to right.
Q7
A7
Why is a temperature gradient required?
The annealing temperature of the specific primers used in DNA amplification often
requires optimisation. Instead of running multiple experiments with different annealing
3
temperatures the gradient thermal cycler ( PrimeG) allows the testing of up to 8 different
annealing temperatures simultaneously.
49
TECHNICAL SUPPORT AND SERVICING
If you require further technical or application assistance please contact Techne at:
E-mail: [email protected]
Phone: +44 (0)1785 810433
Fax: +44 (0)1785 810405
For servicing information please contact:
Service Department
Bibby Scientific Ltd.
Beacon Road
Stone
Staffordshire
ST15 0SA
E-mail: [email protected]
Phone: +44 (0)1785 810475
Fax: +44 (0)1785 810405
In the USA please contact [email protected] or call +1 609 589 2560.
We are continually striving to improve our products and software. If you have any comments and
suggestions on how we can do things better please send them to us at: [email protected]
50
ADDITIONAL INFORMATION
USER MAINTENANCE
3
Before cleaning your unit, disconnect it from the power supply. The outer case of the Prime may be
cleaned with a cloth dipped in water or ethanol (hexane or 50% methanol can also be used). No part
of the case or cover should be immersed in the solvents. Do not use aggressive solvents such as
acetone or abrasive cleaners. The block may be wiped with water, ethanol or propan-2-ol and may
be decontaminated by wiping with 2% Neutracon or 1% bleach solution.
Before using any cleaning or decontamination method except those recommended here, the
responsible person should check with Techne that the proposed method will not damage the
equipment.
FAULT FINDING
Note that this equipment should only be dismantled by properly trained personnel. Removing the
outer cover exposes potentially lethal mains voltages. There are no user serviceable parts within this
equipment.
3
Should you have any problems with your Prime unit which cannot be easily remedied, you should
contact your supplier and return the unit if necessary. Please include details of the fault observed and
remember to return the unit in its original packing. Bibby Scientific Ltd. accepts no responsibility for
damage to units which are not properly packed for shipping: if in doubt, contact your supplier, giving
the full serial number of the unit.
FUSES
If the display on the front panel is not lit, one of the two fuses may have blown. Check that there is no
external cause, such as a faulty plug or lead. Check both fuses and replace the faulty fuse with a
new one of the correct value (fuse values are given on the label next to the power inlet). Note that
fuses should only be replaced by a qualified electrician.
The holder for the two fuses is built into the mains input socket. First remove the power cable and
then gently prise the fuse drawer open with a flat-bladed screwdriver or similar tool. Each fuse can be
removed by using the screwdriver as a lever.
Exchange the faulty fuse in the fuse holder for a working fuse of the correct value. Finally, replace
the fuse drawer in the fuse compartment and push the drawer shut. Fuses which blow repeatedly
indicate a serious fault and you should return the unit to your supplier for repair.
INSULATION TESTING
This equipment is fitted with RFI suppression circuitry. Any check of the electrical insulation by
means of high voltage dielectric testing (for example as in BS EN 61010-1) must be carried out using
only a DC voltage.
This unit contains semiconductor components which may be damaged by electric field effects.
51
ACCESSORIES
The following accessories can be obtained from Bibby Scientific Ltd. or your Techne distributor:
Product code
3PRIMEX/USB
Description
3
PrimeX gradient upgrade
REPLACEMENT PARTS
The following replacement parts can be obtained from Bibby Scientific Ltd. or your Techne
distributor:
Product code
3PRIMEBASE/02/B
3PRIMEBASE/05/B
3PRIMEX/02/B
3PRIMEX/05/B
HH179(S)
HH180(S)
FCABLEUS
Description
3
Prime block, 24 x 0.2ml
3
Prime block, 18 x 0.5ml
3
PrimeX block, 48 x 0.2ml
3
PrimeX block, 30 x 0.5ml
UK 230V mains lead with plug
European mains lead with plug
US mains lead with plug
Note: Exchange of thermal cycler blocks must be carried out by a trained service engineer.
52
53
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