TC-3000G Manual

TC-3000G Manual
TC-3000G
OPERATOR'S MANUAL
Issue 2
6/09
CONTENTS
PAGE
INTRODUCTION
1
SAFETY AND INSTALLATION
2
SÉCURITÉ ET CONSIGNES D'INSTALLATION
5
SICHERHEITS- UND INSTALLATIONSINFORMATIONEN
INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONE
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11
INFORMACIÓN DE SEGURIDAD E INSTALACIÓN
14
BEFORE SWITCHING ON
16
CONTACT INFORMATION
17
TC-3000G SPECIFICATION
18
USER OPERATION AND CONSUMABLES
Heated lid
Tubes or reaction vessels
0.5ml tubes
Switching on
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FRONT PANEL CONTROLS
The LCD display
Indicators
The keys
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PROGRAMMING THE TC-3000G
Programming Options
Option 1: Creating programs using templates
Option 2: Creating a new program
Example programs
Programming
To program a gradient
To program increment or decrement temperature or time
To run a program
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25
25
29
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30
34
37
40
INFORMATION
42
CONFIGURATION
42
GRADIENT CALCULATOR
46
RUNNING THE TC-3000G FROM A PC
Connecting one unit to a PC
Connecting several TC-3000G units to a PC
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47
47
NOTES ON TRANSFERRING PROTOCOLS FROM OTHER THERMAL CYCLERS
48
USER MAINTENANCE
Cleaning your TC-3000G
Heater block removal
49
49
49
SOME FREQUENTLY ASKED QUESTIONS
50
ADDITIONAL INFORMATION
Fault Finding
Fuses
The heated lid over-temperature cut-out
Insulation testing
Interchangeable blocks
Accessories
Replacement parts
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51
51
51
51
51
52
53
INTRODUCTION
The TC-3000G provides the researcher with the means of accurately controlling the temperature profile
of samples. It has many scientific applications, including PCR1 and sequencing. The unit can cycle samples
between 4°C and 99°C.
The TC-3000G is programmed by means of an integral keypad and LCD display. A program, which can
be recalled from memory, consists of:
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a series of specified temperatures in °C.
the times for which each specified temperature will be held (Hold Times).
the desired heating or cooling rates, in °C/sec, between each specified temperature.
optional gradient of temperatures across the heater block.
optional instructions as to whether the times and/or the temperatures are to increase or
decrease when a cycle is repeated.
The memory can store up to 80 programs.
Before using the thermal cycler, make sure you have read this manual carefully. If there is any doubt
relating to the proper use of this equipment, the staff at Bibby Scientific Ltd or your supplier will be happy
to assist you.
1
Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and
development and applied fields other than human in-vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC.
1
SAFETY AND INSTALLATION
OPERATOR SAFETY
Please read this manual carefully before using the thermal cycler. If the equipment is not used in the
manner described in this manual the protection provided by the equipment might be impaired.
All Bibby Scientific Ltd instruments are designed to conform to international safety requirements and are
fitted with an over-temperature cut-out. If a safety problem should be encountered then switch off the
unit at the mains socket and remove the plug from the electricity supply.
WARNING
HIGH TEMPERATURES ARE DANGEROUS as they can cause serious burns to operators and ignite
combustible material. Users should be aware of the following potential hazards:
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USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS
DO NOT use combustible substances near hot objects
DO NOT operate the instrument in the vicinity of inflammable liquids or gases
DO NOT place any liquid directly into the instrument, always use the appropriate
plastic consumables
After heating samples, remember that parts of the instrument, namely the lid and block and any
associated accessories may be very hot.
UNPACKING
When unpacking please ensure that the following have been removed from the packaging:
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Thermal cycler
Mains cable
Guarantee card
Decontamination certificate
The user is advised to keep the original packaging in case the instrument ever needs to be returned for
service or repair. Bibby Scientific Ltd accepts no responsibility for damage incurred unless the unit is
correctly packed and transported in its original packaging.
Please note that the screen of the thermal cycler is easily damaged by sharp objects such as pens, pencils
and fingernails. This type of damage will be considered as misuse and invalidate the guarantee for this
component.
INSTALLATION
The instrument should be carried using both hands. Never move or carry the instrument when in use or
connected to the mains electricity supply.
1. All Bibby Scientific Ltd instruments are supplied with a power cable; this may be integral or plug-in.
2
2. Before connecting the instrument to the mains electricity supply, check the voltage against the rating
plate (located on the back of the unit). Please note that the unit must be earthed to ensure proper
electrical safety. The TC-3000G has a switch-mode power supply which is suitable for use with
universal AC input voltages between 100 and 230V. Connect the mains cable to a suitable plug
according to the table below.
Connections
Live
Neutral
Earth
220V-240V, 50/60Hz Supply
brown
blue
green/yellow
110V-120V, 50/60Hz Supply
black
white
green
3. UK ONLY: The fused plug supplied with the mains cable is fitted with a 10 Amp fuse to protect the
instrument and the user.
4. Plug the mains cable into the socket on the back of the instrument.
5. Place the unit on a suitable flat bench or in a fume cupboard if required, ensuring that the air inlet
vents on the underside are free from obstruction. Position the instrument with a minimum distance all
round of 200mm from walls or other items and between each unit if multiple units are being used to
permit efficient air flow for each instrument.
6. Switch on the instrument:
I mains switch On
O mains switch Off
Replacement cable
Should the mains lead need replacement, a cable of 1mm2 of harmonized code H05VV-F connected to
an IEC320 plug should be used. IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN.
WORKING CONDITIONS
The thermal cycler is designed operate under the following conditions:
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Indoor use
Ambient temperature range +5°C to +40°C
Altitude to 2000m
Up to 95% relative humidity, non-condensing
Mains supply fluctuations not exceeding 10%
Over voltage category II IEC 60364-4-443
Pollution degree 2
Note: The control specifications are quoted at an ambient temperature of 20°C. The specification may
deteriorate outside an ambient temperature of 10°C to 30°C.
The instrument has been tested for radio frequency interference and is certified under EN61326.
3
GUARANTEE
The instrument is guaranteed against any defect in material or workmanship for the period specified on
the enclosed guarantee card. This period is effective from the date of purchase; within this period all
defective parts will be replaced free of charge provided that the defect is not the result of an accident,
misuse or negligence. The screen of the thermal cycler can be damaged by sharp objects such as pens,
pencils and fingernails. Damage of this sort will be considered misuse and will invalidate the guarantee
for this component.
Servicing under this guarantee should be obtained from the supplier of the instrument.
This manual has been prepared for the convenience of Bibby Scientific Ltd's customers and nothing in
this manual shall be taken as a warranty, condition or representation concerning the description,
merchantability, fitness for purpose or otherwise of the unit or components.
Notwithstanding the description and specification(s) of the instruments contained in the operator's
manual, Bibby Scientific Ltd reserves the right to make such changes as it sees fit to the instruments or
to any of the components.
4
SÉCURITÉ ET CONSIGNES D'INSTALLATION
SÉCURITÉ DE L’OPÉRATEUR
Lire attentivement le présent manuel avant d'utiliser le cycleur thermique. Si ce matériel n'est pas utilisé
de la manière décrite dans le présent manuel, la protection fournie risque d'être compromise.
Tous les appareils Bibby Scientific Ltd sont conçus pour être conformes aux exigences internationales de
sécurité et sont dotés d'une coupure en cas de surchauffe. Si un problème de sécurité survient, mettre
l'appareil hors tension au niveau de la prise secteur et débrancher la fiche de l'alimentation électrique.
AVERTISSEMENT
Les TEMPÉRATURES ÉLEVÉES SONT DANGEREUSES car elles peuvent provoquer de graves brûlures chez
l'opérateur et enflammer les matériaux combustibles. Les utilisateurs devront être conscients des dangers
potentiels suivants :
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AGIR AVEC PRUDENCE ET PORTER DES GANTS DE PROTECTION POUR LES MAINS
NE PAS utiliser de substances combustibles à proximité d'objets chauds
NE PAS utiliser l'appareil au voisinage de liquides ou de gaz inflammables
NE PAS placer un quelconque liquide directement sur l'appareil; toujours utiliser les
consommables en plastique appropriés
Rappel : après le chauffage d'échantillons, certaines parties de l'appareil, notamment le couvercle et le
bloc, ainsi que les accessoires associés, peuvent être brûlants.
DÉBALLAGE
Au moment du déballage, vérifier que l'emballage contenait les articles suivants :
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Cycleur thermique
Câble secteur
Carte de garantie
Certificat de décontamination
Il est conseillé à l'utilisateur de conserver l'emballage d'origine s'il devait s'avérer nécessaire de renvoyer
l'appareil pour une intervention d'entretien ou de réparation. Bibby Scientific Ltd n'accepte aucune
responsabilité pour les dégâts survenus si l'appareil n'est pas correctement emballé et transporté dans
sont emballage d'origine.
Noter que des objets pointus tels que crayons, stylos et ongles peuvent facilement endommager l'écran
du cycleur thermique. Ce type de dégâts sera considéré comme une mauvaise utilisation et annulera la
garantie de ce composant.
INSTALLATION
Porter l'appareil à deux mains. Ne jamais déplacer ou transporter l'appareil lorsqu'il est en service ou
branché à l'alimentation électrique sur secteur.
5
1. Tous les appareils Bibby Scientific Ltd sont livrés avec un câble d'alimentation, qui peut être intégré à
l'appareil ou muni d'une fiche.
2. Le TC-3000G a une alimentation électrique avec un sélecteur de tension qui vous permet un choix
entre 90 et 260V. Reliez le câble d'alimentation à une prise appropriée selon la table ci-dessous.
Connexions
Phase
Neutre
Terre
Alimentation 220V-240 V, 50/60 Hz
marron
bleu
vert/jaune
Alimentation 110V-120 V, 50/60 Hz
noir
blanc
vert
3. ROYAUME-UNI SEULEMENT: La prise à fusible fournie avec le câble secteur est munie d'un fusible 10
A destiné à protéger l'appareil et l'utilisateur.
4. Brancher le câble secteur dans la prise au dos de l'appareil.
5. Disposer l'appareil sur une paillasse ou sous une hotte aspirante si nécessaire, en veillant à ce que les
prises d'air situées au-dessous ne soient pas obstruées. Positionner l'appareil avec tout autour un
écartement minimum de 200 mm par rapport aux murs ou autres éléments, ainsi qu'entre chaque
appareil en cas d'utilisation d'appareils multiples, afin de permettre une circulation d'air efficace pour
chaque appareil.
6. Allumer l'appareil.
I Interrupteur secteur en position Marche
O Interrupteur secteur en position Arrêt
Câble de rechange
S'il s'avère nécessaire de remplacer le cordon secteur, utiliser un câble de 1 mm2 au code harmonisé
H05VV-F raccordé à une fiche IEC320. EN CAS DE DOUTE, CONSULTER UN ÉLECTRICIEN QUALIFIÉ.
CONDITIONS DE TRAVAIL
Le cycleur thermique est conçu pour fonctionner dans les conditions suivantes:
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Utilisation en intérieur
Plage de température ambiante : +5°C à +40°C
Altitude jusqu'à 2000 m
Au-dessus de 95% d'humidité relative, pas de condensation
Fluctuations de l'alimentation sur secteur ne dépassant pas 10%
Surtensions de catégorie II selon la norme IEC 60364-4-443
Degré de pollution 2
Remarque: Les caractéristiques de régulation sont indiquées à une température ambiante de 20°C. Ces
caractéristiques peuvent se détériorer en dehors d'une température ambiante de 10°C à 30°C.
L'appareil a été testé en matière de perturbations radioélectriques et est certifié selon la norme EN61326.
6
GARANTIE
L'appareil est garanti contre tout défaut de matériaux ou vice de fabrication pendant la période précisée
sur la carte de garantie jointe. Cette période s'applique à compter de la date d'achat; pendant cette
période, toutes les pièces défectueuses seront remplacées gratuitement à condition que le défaut ne soit
pas le résultat d'un accident, d'une mauvaise utilisation ou d'une négligence. Des objets pointus tels que
crayons, stylos et ongles peuvent endommager l'écran du cycleur thermique. Les dégâts de cette sorte
seront considérés comme une mauvaise utilisation et annuleront la garantie de ce composant.
Dans le cadre de cette garantie, le service après-vente est à obtenir auprès du fournisseur de l'appareil.
Le présent manuel a été préparé pour le confort des clients de Bibby Scientific Ltd et rien dans son
contenu ne doit être pris comme une garantie, une condition ou une affirmation concernant la
description, la qualité marchande, l'adéquation à un usage particulier ou autre de l'appareil ou de ses
composants.
Nonobstant la description et les caractéristiques techniques des appareils figurant dans le manuel de
l'utilisateur, Bibby Scientific Ltd se réserve le droit d'apporter aux appareils ou à n'importe quel composant
les changements jugés utiles.
7
SICHERHEITS - UND INSTALLATIONSINFORMATIONEN
SICHERHEIT DES BEDIENPERSONALS
Lesen Sie diese Anleitung vor Verwendung des Thermocyclers bitte sorgfältig durch. Wenn das Gerät
nicht entsprechend der Bedienungsanleitung eingesetzt wird, können die Schutzfunktionen des Gerätes
beeinträchtigt werden.
Alle Geräte von Bibby Scientific Ltd entsprechen den internationalen Sicherheitsanforderungen und sind
mit einer Übertemperatur-Schutzvorrichtung ausgestattet. Bei einer Sicherheitsstörung bitte das Gerät an
der Steckdose ausschalten und den Netzstecker ziehen.
ACHTUNG
HOHE TEMPERATUREN STELLEN EINE GEFAHRENQUELLE DAR. Sie können schwere Brandverletzung
verursachen und brennbare Stoffe entzünden. Der Benutzer sollt sich mit den möglichen Gefahren
vertraut machen:
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UMSICHTIG VORGEHEN UND SCHUTZHANDSCHUHE TRAGEN
KEINE brennbaren Stoffe in der Nähe heißer Gegenstände verwenden
Das Gerät NICHT in der Nähe entzündlicher Flüssigkeiten oder Gase betreiben
Flüssigkeiten NICHT direkt auf das Gerät auftragen - verwenden Sie stets die
entsprechenden Kunststoffeinsätze
Nach der Erhitzung von Proben daran denken, dass bestimmte Geräteteile wie Deckel, Thermoblock und
Zubehörteile sehr heiß sein können.
AUSPACKEN
Beim Auspacken darauf achten, dass der folgende Lieferumfang vorhanden ist:
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Thermocycler
Netzkabel
Garantiekarte
Dekontaminationszertifikat
Wir empfehlen die Originalverpackung aufzubewahren, falls das Gerät zwecks Wartung oder Reparatur
zurückgeschickt werden muss. Bibby Scientific Ltd übernimmt keine Verantwortung für
Transportschäden, wenn das Gerät nicht ordnungsgemäß verpackt in der Originalpackung verschickt
wird.
Achten Sie bitte darauf, dass die Anzeige des Thermocyclers durch spitze bzw. scharfe Gegenstände wie
Stifte, Bleistifte und Fingernägel leicht zerkratzt wird. Diese Art von Beschädigung gilt als Missbrauch des
Geräts und führt zur Ungültigkeit der Garantie für dieses Gerät.
INBETRIEBNAHME
Das Gerät mit beiden Händen tragen. Das Gerät unter keinen Umständen transportieren, wenn es in
Betrieb ist, oder während das Gerät noch am Netz angeschlossen ist.
8
1. Alle Geräte von Bibby Scientific Ltd werden mit einem Netzkabel geliefert, das entweder eingesteckt
wird oder fest mit dem Gerät verbunden ist.
2. Der TC3000G hat einen Switch-Mode Power Supply, der mit universellen AC Eingangsspannungen
zwischen 90 und 260V genutzt werden kann. Schliessen Sie das Netzkabel an eine geeignete
Steckdose an, entsprechend der unten aufgeführten Tabelle.
Anschluss
Phase
Neutral
Erde
220/240V, 50/60Hz
Braun
Blau
Grün/Gelb
110V/120V, 50/60Hz
Schwarz
Weiß
Grün
3. NUR FÜR GROSSBRITANNIEN: der mit dem Netzkabel gelieferte Sicherungsstecker enthält eine 10
Amp. Sicherung zum Schutz des Geräts und des Anwenders.
4. Stecken Sie das Netzkabel in die Buchse auf der Geräterückseite ein.
5. Stellen Sie das Gerät auf einen geeigneten ebenen Tisch oder in einem Abzugsschrank auf und sorgen
Sie dafür, dass die Lufteinlassschlitze auf der Geräteunterseite nicht blockiert sind. Das Gerät muss
einen Mindestabstand von 200 mm zu Wänden und anderen Gegenständen bzw. zu anderen Geräten
dieser Art aufweisen, um einen ausreichenden Luftstrom zu gewährleisten.
6. Schalten Sie das Gerät ein:
I
Netzschalter Ein
O Netzschalter Aus
Ersatzkabel
Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1mm2
Adernquerschnitt und Europastecker (IEC 320) benötigt. IM ZWEIFELSFALL EINEN ELEKTROFACHMANN HINZUZIEHEN.
EINSATZBEDINGUNGEN
Der Thermocycler ist für den Einsatz unter folgenden Bedingungen ausgelegt:
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Gebrauch in Innenräumen
Umgebungstemperatur zwischen +5°C to +40°C
Höhe: bis zu 2000 m
Relative Feuchte nicht über 95%
Netzspannungsschwankungen nicht über 10%
Überspannungsklasse 2 IEC 60364-4-443
Verschmutzungsgrad 2
Hinweis: Die Gerätespezifikationen beziehen sich auf eine Umgebungstemperatur von 20°C und können
sich außerhalb des Bereichs 10°C bis 30°C verschlechtern.
Das Gerät wurde auf HF-Störeinflüsse geprüft und entspricht den EMV-Bedingungen nach EN61326.
9
GARANTIE
Bibby Scientific Ltd gewährleistet, dass dieses Gerät für den auf der Garantiekarte angegebenen Zeitraum
keine Herstellungs- und Materialmängel aufweist. Dieser Zeitraum tritt ab dem Verkaufsdatum in Kraft.
Innerhalb dieses Zeitraums werden alle defekten Teile kostenlos ausgetauscht, soweit der Defekt nicht auf
einen Unfall, Missbrauch oder Nachlässigkeit zurückzuführen ist. Die Anzeige des Thermocylers kan durch
spitze/scharfe Gegenstände wie Stifte, Bleistifte und Fingernägel beschädigt werden. Diese Art von
Beschädigung gilt als Missbrauch des Geräts und führt zur Ungültigkeit der Garantie für dieses Gerät.
Wartungsarbeiten, die unter diese Garantie fallen, müssen von der Verkaufsstelle für dieses Gerät
gehandhabt werden.
Diese Anleitung wurde zur Information der Kunden von Bibby Scientific Ltd erstellt und stellt in keinster
Weise eine Gewährleistung, Bedingung oder Darstellung bezüglich der Beschreibung, Marktgängigkeit
oder Zweckdienlichkeit dieser Geräte oder Bauteile dar.
Unabhängig von Beschreibung und Spezifikation(en) des hier beschriebenen Geräts behält sich Bibby
Scientific Ltd das Recht vor, Änderungen an diesem Gerät oder dessen Bauteilen vorzunehmen.
10
INFORMACIÓN DE SEGURIDAD E INSTALACIÓN
SEGURIDAD DEL OPERARIO
Lea atentamente este manual antes de utilizar el ciclador térmico. Si el equipo no se utiliza de la forma
descrita en este manual, se reducirá la protección ofrecida por el equipo.
Todos los instrumentos Bibby Scientific Ltd están diseñados para cumplir los requisitos internacionales de
seguridad, e incluyen un dispositivo de corte de sobretemperatura. Si se produce un problema de
seguridad, apague la unidad en la toma de alimentación y retire el enchufe del suministro eléctrico.
ADVERTENCIA
LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que pueden ocasionar quemaduras graves a los
operarios y prender el material combustible. Los usuarios deben conocer los posibles riesgos:
• TENGA CUIDADO Y LLEVE GUANTES DE PROTECCIÓN PARA PROTEGERSE LAS
MANOS
• NO utilice sustancias combustibles cerca de objetos calientes
• NO utilice el instrumento cerca de líquidos o gases inflamables
• NO coloque un líquido directamente en el instrumento. Utilice siempre los
consumibles plásticos adecuados
Después de calentar las muestras, recuerde que hay componentes del instrumento que pueden calentarse
mucho, concretamente la tapa y el cuerpo y todos los accesorios asociados.
DESEMBALAJE
Durante el desembalaje, asegúrese de sacar los siguientes componentes del embalaje:
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Ciclador térmico
Cable de alimentación
Tarjeta de garantía
Certificado de descontaminación
Se recomienda guardar el embalaje original en caso de que tenga que enviar el instrumento para un
trabajo de mantenimiento o reparación. Bibby Scientific Ltd no se responsabiliza de los daños producidos
si la unidad no está debidamente embalada y no se envía en su embalaje original.
Tenga en cuenta que los objetos punzantes, p.ej.: bolígrafos, lápices y uñas, pueden dañar fácilmente la
pantalla del ciclador térmico. Este tipo de daño se considerará como un uso incorrecto, e invalidará la
garantía de este componente.
INSTALACIÓN
El instrumento se debe transportar con las dos manos. No mueva ni lleve el instrumento cuando se utilice
o esté conectado al suministro eléctrico principal.
1. Todos los instrumentos Bibby Scientific Ltd se suministran con un cable de alimentación, que puede ser
integrado o 'enchufable'.
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2. EI TC-3000G tiene una fuente conmutada que es conveniente para el uso con voltajes de entrada
universales de CA entre 90 y 260V. Conecte el cable de alimentación a un enchufe adecuado según la
siguiente tabla.
Conexión
Con corriente
Neutro
Toma de tierra
220/240V, 50/60Hz Supply
marrón
azul
verde/amarillo
110V/120V Supply, 50/60Hz
negro
blanco
verde
3. SÓLO PARA EL REINO UNIDO: El enchufe suministrado con el cable de alimentación incluye un fusible
de 10 amperios para ofrecer protección al instrumento y al usuario.
4. Conecte el cable de alimentación en el enchufe situado en la parte trasera del instrumento.
5. Sitúe la unidad sobre una mesa plana o en una campana de laboratorio si es necesario, y asegúrese de
que los orificios de ventilación situados en la parte inferior no tienen ninguna obstrucción. Coloque el
instrumento a una distancia mínima de 200 mm alrededor de paredes u otros elementos, y entre cada
unidad (en caso de que se utilicen varias) para que haya una circulación de aire adecuada para cada
instrumento.
6. Encienda el instrumento:
I Interruptor de alimentación encendido
O Interruptor de alimentación apagado
Cable de repuesto
Si es necesario sustituir el cable de alimentación, se debe utilizar un cable de 1mm2 de código armonizado
H05VV, conectado a un enchufe IEC320. EN CASO DE DUDA, PÓNGASE EN CONTACTO CON UN
ELECTRICISTA.
CONDICIONES DE TRABAJO
El ciclador térmico está diseñado para utilizarse en las condiciones siguientes:
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•
•
•
•
•
Uso en interior
Intervalo de temperatura ambiente +5°C a +40°C
Humedad relativa no superior al 95%
Fluctuaciones del suministro eléctrico no superiores al 10%
Categoría de sobrevoltaje II IEC 60364-4-443
Nivel de contaminación 2
Nota: Las especificaciones de control corresponden a una temperatura ambiental de 20ºC. Las
especificaciones pueden empeorar si se utiliza el instrumento fuera del intervalo de temperatura
comprendido entre 10°C y 30°C.
Se han realizado pruebas para comprobar la interferencia de radiofrecuencia del instrumento, el cual
cumple la normativa EN61326.
12
GARANTÍA
El instrumento está garantizado contra cualquier defecto en el material o la fabricación durante el
período especificado en la tarjeta de garantía que se adjunta. Este período entra en vigor a partir de la
fecha de compra. Durante este período, se reemplazarán sin cargo alguno todas las piezas defectuosas,
a condición que el defecto sea resultado de un accidente, uso incorrecto o negligencia. Tenga en cuenta
que los objetos punzantes, p.ej.: bolígrafos, lápices y uñas, pueden dañar fácilmente la pantalla del
ciclador térmico. Este tipo de daño se considerará como un uso incorrecto, e invalidará la garantía de este
componente.
El distribuidor del instrumento proporcionará información sobre las reparaciones realizadas bajo esta
garantía.
Este manual se ha preparado con una finalidad informativa para los clientes de Bibby Scientific Ltd, y
ninguna parte del manual se deberá considerar como una garantía, condición o reflejo con respecto a la
descripción, comerciabilidad, idoneidad para un fin determinado o de otro tipo de la unidad o sus
componentes.
Con independencia de la descripción y las especificaciones del instrumento que se indican en el manual
del operario, Bibby Scientific Ltd se reserva el derecho de realizar cambios en el instrumento o en
cualquiera de sus componentes cuando lo estime oportuno.
13
INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONE
SICUREZZA DELL'OPERATORE
Leggere attentamente il presente manuale prima di usare il ciclatore termico. Se non si usa
l'apparecchiatura nel modo descritto nel presente manuale, la protezione fornita dall'unità potrebbe
risultarne diminuita.
Tutti gli strumenti Bibby Scientific Ltd sono progettati per rispettare i requisiti di sicurezza internazionali e
sono montati con un sezionatore di sovratemperatura. In caso di problemi di sicurezza, spegnere l'unità
dalla presa di rete centrale e togliere la spina dall'alimentazione elettrica.
AVVERTENZA
Le ALTE TEMPERATURE SONO PERICOLOSE in quanto possono provocare serie ustioni agli operatori e
dare fuoco al materiale combustibile. Gli utenti devono conoscere i seguenti pericoli potenziali:
•
•
•
•
PRESTARE ATTENZIONE ED INDOSSARE GUANTI PROTETTIVI PER LE MANI
NON usare sostanze combustibili vicino ad oggetti caldi
NON mettere in funzione lo strumento nei pressi di liquidi o gas infiammabili
NON collocare alcun tipo di liquido direttamente nello strumento, usare sempre le
parti di consumo in plastica idonee
Dopo aver riscaldato i campioni, ricordare che le parti dello strumento, cioè il coperchio ed il blocco ed
eventuali accessori associati potrebbero essere molto caldi.
DISIMBALLAGGIO
Durante il disimballaggio, assicurarsi di aver tolto quanto segue dall'imballo:
•
•
•
•
Ciclatore termico
Cavo di alimentazione di rete
Scheda di garanzia
Certificato di decontaminazione
Si consiglia all'utente di conservare l'imballaggio originale nel caso in cui occorresse restituire lo
strumento per assistenza o riparazioni. Se l'unità non è correttamente imballata e trasportata nel suo
imballo originale, Bibby Scientific Ltd non accetta alcuna responsabilità per eventuali danni che dovessero
verificarsi.
Notare come lo schermo del ciclatore termico si danneggia facilmente con oggetti appuntiti come penne,
matite ed unghia. Questo tipo di danni sarà considerato come uso improprio e renderà nulla la garanzia
per questo componente.
INSTALLAZIONE
Occorre trasportare lo strumento usando entrambe le mani. Non spostare né trasportare lo strumento
quando è in funzione o collegato all'alimentazione elettrica di rete.
1. Tutti gli strumenti Bibby Scientific Ltd sono forniti con un cavo di alimentazione; può essere integrale
o plugin.
14
2. Il modello TC-3000G dispone di alimentazione idonea per voltaggi in corrente alternata tra 90 e
260V. Collegare il cavo di alimentazione ad una spine idonea in accordo alla tabella sottoriportata.
Connessione
Sotto tensione
Neutro
Terra
220/240V, Alimentazione 50/60Hz
marrone
blu
verde/giallo
Alimentazione 110V/120V, 50/60Hz
nero
bianco
verde
3. SOLO REGNO UNITO: la spina con fusibile fornita con il cavo di rete è dotata di un fusibile da 10 Amp
per proteggere lo strumento e l'utente.
4. Inserire il cavo di rete nella presa che si trova sul retro dello strumento.
5. Collocare l'unità su un banco piatto idoneo o in una cappa aspirante se necesario, assicurandosi che
gli sfiati delle prese d'aria nella parte inferiore non siano ostruiti. Posizionare lo strumento con una
distanza minima attorno di 200 mm. dalle pareti o da altri oggetti e tra ciascuna unità, nel caso in cui
si usino unità multiple, in modo da consentire un flusso di'aria efficace per ciascuno strumento.
6. Accendere lo strumento:
I Interruttore di rete Acceso
O Interruttore di rete Spento
Cavo di ricambio
Qualora occorra sostituire il cavo di rete, si dovrà utilizzare un cavo di 1mm2 codice armonizzato H05VV-F
collegato ad una spina IEC 320. IN CASO DI DUBBIO, RIVOLGERSI A UN ELETTRICISTA
QUALIFICATO.
CONDIZIONI DI ESERCIZIO
Il ciclatore termico è stato progettato per funzionare nelle seguenti condizioni:
•
•
•
•
•
•
•
uso interno
range di temperatura ambiente da +5°C a +40°C
altitudine massima 2000 m.
umidità relativa non superiore all'95%
oscillazione dell'alimentazione di rete non superiore al 10%
categoria di sovratensione II IEC 60364-4-443
grado di inquinamento 2
Nota: le specifiche di controllo sono indicate ad una temperatura ambiente di 20°C. Le specifiche
potrebbero peggiorare fuori da una temperatura ambiente compresa tra 10°C e 30°C.
Lo strumento è stato collaudato per interferenze da radiofrequenze ed è certificato secondo la norma
EN61326.
15
GARANZIA
Lo strumento è garantito da qualsiasi difetto nei materiali o nella lavorazione per il periodo specificato
nella scheda di garanzia allegata. Questo periodo è valido dalla data di acquisto; entro tale periodo, tutte
le parti difettose saranno sostituite gratuitamente, a condizione che il difetto non sia la conseguenza di
un incidente, un uso improprio o negligenza. Lo schermo del ciclatore termico può essere danneggiato
da oggetti appuntiti come penne, matite ed unghia. Tale tipo di danno sarà considerato uso improprio e
renderà nulla la garanzia per questo componente.
L'assistenza secondo quanto stabilito dalla presente garanzia deve essere ottenuta dal fornitore dello
strumento.
Il presente manuale è stato preparato ad uso dei clienti di Bibby Scientific Ltd e niente di quanto in esso
contenuto costituisce garanzia, condizione o rappresentanza riguardo la descrizione, la commerciabilità,
l'idoneità allo scopo o altrimenti dell'unità o dei componenti.
Nonostante la descrizione e le specifiche dello strumento contenuti nel manuale dell'operatore, Bibby
Scientific Ltd si riserva il diritto di apportare le modifiche ritenute opportune agli strumenti o a qualsiasi
loro componente.
BEFORE SWITCHING ON
TC-3000G
USB
On/Off switch
Power in - no requirement to change
fuses as a switch-mode power supply
is fitted.
16
CONTACT INFORMATION
For technical, sales or servicing information, contact your local Bibby Scientific Ltd dealer or:
UK and Rest of World
Bibby Scientific Ltd
Beacon Road, Stone
Staffordshire ST15 0SA, UK
Tel: +44 (0)1785 812121
Fax: +44 (0)1785 813748
e-mail: [email protected]
www.bibby-scientific.com
North and South America
Techne Inc.
3 Terri Lane, Suite 10
Burlington, N.J. 08016, USA
Tel: 800-225-9243
Fax: 609-589-2571
e-mail: [email protected]
www.techneusa.com
France
Bibby Scientific France SAS
ZI du Rocher Vert - BP 79
77793 Nemours Cedex
France
Tel: +33 1 64 45 13 13
Fax: +33 1 64 45 13 00
e-mail: [email protected]
www.bibby-scientific.com
Italy
Bibby Scientific Italia Srl
Via Alcide de Gasperi 56
20077 Riozzo di Cerro al Lambro
Milano, Italia
Tel: +39 (0)2 98230679
Fax: +39 (0)2 98230211
e-mail: [email protected]
www.bibby-scientific.it
Middle East
Techne Middle East Ltd.
PO Box 27887, Engomi 2433
Nicosia
Cyprus
Tel: +357 22 660427
Fax: +357 22 660356
e-mail: [email protected]
Far East
Bibby Scientific (Asia) Ltd
Room 607 Yen Sheng Centre
64 Hoi Yuen Road Kwun Tong
Kowloon Hong Kong
Tel: +852 3583 1581
Fax: +852 3583 1580
e-mail [email protected]
www.bibby-scientificasia.com
17
TC-3000G SPECIFICATION
Temperature
Gradient range
Maximum gradient
Minimum gradient
Temperature range
Temperature set point precision
Block uniformity (over full range)
Temperature accuracy
Pre-run sample cooling
End of program cooling below ambient
20°C to 80°C
15°C
1°C
4°C to 99°C
0.1°C
± 0.2°C
± 0.2°C
Yes
Yes
Heating/Cooling Rate
Heating rate, between 55°C and 90°C
on a 50°C to 95°C step:
0.2 ml micro tubes
0.5 ml micro tubes
Cooling rate, between 90°C and 55°C
on a 95°C to 50°C step:
0.2 ml micro tubes
0.5 ml micro tubes
3.3°C/sec
3.3°C/sec
2.0°C/sec
2.0°C/sec
Heated Lid
Adjustable heated lid
Heated lid enable/disable
Selectable heated lid temperature
Over-temperature cut-out
Temperature sensor
Heater type
Heater power
Warm up time
Yes
Yes
100°C to 115°C
Fixed at 145°C
Thermistor
Etched foil
33W
4 min approximately
(ambient to 105°C)
The heated lid will only come on
if the set temperature is above 35°C
18
Programming
Program naming using alpha keys
Program password protection
Number of programs (3 step)
Maximum number of steps per stage
Maximum number of stages per program
Minimum hold time
Maximum hold time
Incremental/decremental temperature
Incremental/decremental step hold time
Programmable ramp rate (heat or cool)
Programmable ramp rate resolution
Yes
Yes
80
35
25
1 sec
18 hrs
Yes
Yes
Variable in 0.1°C/sec steps
0.1°C
Running Programs
Pause button
Stop button
End of program alarm
Auto restart on power fail
Yes
Yes
Yes
Yes
Interconnectivity
Connection to PC control program
Software updates from Bibby Scientific Ltd web-site
Yes
Yes
Dimensions
Height
Width
Length
185mm
185mm
335mm
Communication Ports
PC connection to run "Gensoft"
the PC control software.
USB
Power
Power consumption
230W
The TC-3000G unit is supplied with a switched mode power supplier so the unit automatically
recognises the input voltage.
19
USER OPERATION AND CONSUMABLES
HEATED LID
The heated lid has a rotating knob to adjust the lid
pressure on the samples, allowing for a variety of
consumables to be used. To adjust the pressure:
• Adjust the orange rotating knob anticlockwise
to give the least pressure.
• Place the samples in the block and place the lid
in the down position.
• Gently rotate the knob clockwise until you can
feel pressure beginning to be applied.
• Rotate the knob a further quarter of a turn and
this will give the correct pressure.
Do not over-tighten the knob as this will cause deformation of the silicone rubber mat (where used) and
reduce the effectiveness of the lid. Once the pressure has been set it does not need to be adjusted unless
a different block or consumable is used.
The silicone rubber mat supplied with the unit (Part number 6106196) is for use with 48 well microplates
when sealing using a plastic adhesive film. The mat should be placed on top of the sealing film to ensure
good thermal contact with the heated lid.
TUBES OR REACTION VESSELS
Bibby Scientific Ltd does not recommend any specific tube or reaction vessel other than those described
in this manual. We recommend using reaction volumes between 20 and 200µl. The tubes must withstand
a pressure of 1 atmosphere at 100°C. Any vessel must be able to withstand the temperatures you are
using without any danger of them deforming to the point where they fracture.
To test your tubes, put 25µl of water into each of 5 tubes and subject them to a typical thermal cycling
protocol. At the end of the program, measure the volume remaining using a micropipette. A loss of more
than 1 or 2µl indicates a vapour leak.
The amount of volume loss you observe and the change in reactant concentrations you can tolerate
determine the minimum volume that can be used. Typical volume losses of 1µl in 30 cycles allow the use
of samples of 20µl or less.
During the final cool-down, a ring of condensation may form above the liquid level but below the top of
the sample block. This is normal and does not effect the reaction.
20
0.5ML TUBES
In order to fit the maximum number of tubes in the block, they need to be placed so that all the hinges
are in the same relative orientation. An example is illustrated below.
Place the tubes in the block in the same
orientation in order to achieve the maximum
capacity.
Always make sure the pressure on the heated
lid is balanced by spreading the tubes across
the block or inserting some dummy tubes if
necessary.
PLATES AND SEALS
The 48 x 0.2ml block of the TC-3000G will accept 48-well plates as well as individual and strip 0.2ml
tubes. To avoid the possibility of sample evaporation Bibby Scientific Ltd recommends that plates are
sealed with appropriate caps or heat seals. We do not recommend the use of adhesive seals, but if their
use is unavoidable then the supplied silicone rubber mat should be placed on top of the sealing film to
ensure good thermal contact with the heated lid.
SWITCHING ON
When you switch on the unit a screen similar
to the one shown here will appear briefly. This
indicates the software version and block type
installed in the unit.
TC-3000G
Version
Block
x.xx
48x0.2ml GRAD
When the initial screen has disappeared the
screen shown here will automatically appear.
There are four options:
Programs
[0]
Information
Configuration
Gradient calculator
[
, to select
]
1. 'Programs' for creating and running
programs;
2. 'Information' for obtaining information
about the unit e.g. serial number;
3. 'Configuration' for setting up program
defaults and settings.
4. Gradient calculator for displaying column
temperatures of a set gradient.
Each of these is explained in detail in the
following sections.
21
FRONT PANEL CONTROLS
Operation or
Program lines
Prompt line
Programs
Information
Configuration
[
, to select
Up
[0]
Decimal Point
& Insert
]
End
Delete
Down
1
Power
4
Alpha/Numeric
keys
ghi
7
pqrs
2
abc
5
jkl
8
tuv
Pause
3
def
i
Status
6
mno
9
0
wxyz
Enter
TC-3000G
THE LCD DISPLAY
Programs
[0]
Information
Configuration
[
, to select
]
The TC-3000G has a four line LCD display. The
top three lines are information or program
lines, while the bottom line is a prompt line.
The active line flashes on screen and is shown
in bold in this manual.
-------- Stage 1 --------Number cycles
10
Step 94.0° C 00m30
max
[ '.' to insert step
]
The bottom line may change or flash between
two or three different prompts depending
upon which program line is active. An
example of a prompt line which flashes
between three different prompts is shown
here.
[
[
,
to edit
to delete step
]
]
INDICATORS
Power: This indicator is lit when there is power to the TC-3000G.
i
Status: This indicator is lit when the unit is running a program. It is off when the unit is
stopped. It will flash and beep slowly when the unit is paused. It will flash after a five second
quick sequence of beeps when the unit reaches its final hold temperature.
22
THE KEYS
The End, Finish or Exit key
The "End" or "Finish" key is used to finish editing
programs or to exit a program. It is also used to
exit a sequence of key operations.
The Up arrow key
The "Up arrow" key is used for scrolling up
through the lines on the screen. It is also used to
increase certain fields when editing.
The Down arrow key
The "Down arrow" key is used for scrolling down
through the lines on the screen. It is also used to
decrease certain fields when editing.
The Decimal Point and Insert key
The "Decimal Point" key is used when editing
numeric fields. It is also used as an Insert key
during program editing to insert additional steps
and other field sets.
The Delete key
The "Delete" key is used during program editing to
delete steps and stages.
The Pause key
The "Pause" key is used during program editing to
insert a pause after a stage, to insert increment
and decrement times and temperatures and to set
a gradient across the block. It is also used to pause
a program when it is running.
The Enter or Accept key
The "Enter" key is used to enter an operation or
the edit mode. It is also used to 'Accept' a field or
program change.
The Alpha/Numeric keys
The "Alpha/Numeric" keys are used to enter
numbers and letters during programming. If it is a
numeric field then numbers will be inserted; if it is
an alpha field then letters will be inserted. Each key
will cycle through the letters shown on the key (0
will insert a space). To insert a number, press and
hold the appropriate key.
1
4
ghi
7
pqrs
2
abc
jkl
5
8
tuv
3
def
6
mno
9
wxyz
0
23
PROGRAMMING THE TC-3000G
The table below illustrates the symbols which appear on the LCD display and the relevant keypad button
to which they refer.
In the prompt line is equivalent to the "End" key.
On a program line means the program is locked;
it can be copied and then edited but not edited directly.
Up arrow
Down arrow
Enter
Pause
The following terms are used when programming the thermal cyclers:
Stage
A 'Stage' is the name given to the main repeated part of a program containing the 'Steps' to be
repeated. A program can contain a maximum of 25 stages although typically only one or two
are used.
Cycle
A 'Cycle' is the number of repetitions of a 'Stage'
Step
A 'Step' is a user defined program line containing a temperature, a hold time and ramp rate.
Typically three steps are created within one 'Stage'.
To enter the programming section of the
software, highlight 'Programs' using the
"Up Arrow" or "Down Arrow" keys
Programs
[0]
Information
Configuration
[
,
to select ]
Press "Enter"
This screen lists all the programs saved on the
unit. Use the "Up Arrow" and "Down Arrow"
keys to scroll through the list.
* * New Program * * [ ]
2 STEP TEMPLATE 501
3 STEP TEMPLATE 502
[
,
to select ]
The number next to the program name is the
unique "Speed dial" number allocated to the
program for rapid retrieval (see below).
24
PROGRAMMING OPTIONS
There are two options available for creating programs on the TC-3000G:
1. Copy and edit one of the pre-written templates.
2. Create a new program from the defaults.
OPTION 1: CREATING PROGRAMS USING TEMPLATES
There are two pre-written basic PCR templates: a two-step and a three-step template. These can be
copied and modified as required.
2 STEP TEMPLATE
Heated Lid
105°C
Preheat Lid
on
Sample cooling
on
Pause
off
------------------------In denat
94°C 5m00
Hot Start
off
------- Stage 1--------Number cycles
30
Step
94.0°C 0m30
max
Step
68.0°C 1m00
max
------------------------Final Extn 72.0°C
5m00
Final hold
10.0°C
3 STEP TEMPLATE
Heated Lid
105°C
Preheat Lid
on
Sample cooling
on
Pause
off
------------------------In denat
94.0°C 5m00
Hot Start
off
------- Stage 1--------Number cycles
30
Step
94.0°C 0m30
max
Step
55.0°C 0m30
max
Step
72.0°C 0m30
max
------------------------Final Extn 72.0°C
5m00
Final hold
10.0°C
Other templates available include Ice bucket (incubation at 4ºC), Ligation (incubation at 15ºC) and an RTPCR template.
Use the "Down Arrow" key
to select an
existing 'Template' program from the menu,
e.g. '2 Step Template' or '3 Step Template'.
* * * New Program * * *
2 STEP TEMPLATE
501
3 STEP TEMPLATE
502
[
,
to select
]
2 STEP TEMPLATE [501]
Run program
View program
[
,
to select
Run program
View program
Copy program
[
,
to select
Press the "Enter" key
to accept it.
The Template is already defined and can be run
as it is or easily copied, modified and saved
under a new name.
]
Use the "Down Arrow" key
to select 'Copy
Program' Press the "Enter" key
to accept it.
]
25
A new name can now be given to the copied
program (see below).
UNNAMED #
Heated Lid
105°C
Preheat Lid
on
[
,
to edit
]
To give a program a name
To type in a name for the program, press
"Enter"
and a cursor will appear in the
position shown.
UNNAMED #
Heated Lid
105°C
Preheat Lid
on
[
,
to edit
]
1
4
ghi
7
pqrs
2
abc
jkl
5
8
tuv
Using the keypad, type in the name you wish
to give the program.
3
def
The letters will scroll round with successive
presses; for example the "2" key will give 'A'
with one press; 'B' with two presses; 'C' with
three presses and '2' with four presses. Five
presses will again give 'A'.
6
mno
9
0
wxyz
When the name is complete, press "Enter"
There are now two options:
DONNA
Heated Lid
Preheat Lid
[
,
[
1. Save the program and run it as it is or
105°C
on
to edit
]
to save
2. Edit the program then save and run it.
]
To save the program
A program can only be run once it has been
saved. Press the "End"
key.
DONNA
Heated Lid
Preheat Lid
[
SAVE?
[
YES or
The prompt line will flash between 'SAVE?'
and 'YES or NO'.
105°C
on
]
NO
Press "Enter"
for 'Yes' to save the changes
you have made.
If you press "End"
for 'No' then all the
changes you have made will be lost.
]
The program is now ready to run and will be
allocated with a unique 'Speed dial' number
which can be used to rapidly locate the
program from the main start-up screen.
DONNA
[7]
Run Program
View Program
[
,
to select
]
The “Up Arrow” or “Down Arrow” keys
can be used to scroll through the remaining
list of options.
26
To edit a field or step in an existing program
Programs
Information
Configuration
[
, to select
DAVID
DONNA
[
When the unit is switched on the display will
show this screen.
[0]
If known, type in the 'Speed dial' number for
the required program and press "Enter"
This will take you directly to the required
program.
]
If you do not know the 'Speed dial' number,
press "Enter"
to access the list of stored
programs.
8 [D]
7
,
to select
Either:
]
1. Scroll to the required program using the
"Up Arrow" or "Down Arrow" keys
or
2. Type the first letter of the program name in
the 'New program' field; this will then list all
the program names beginning with that
letter. Use the "Up Arrow" or "Down
Arrow" keys
to select the required
program.
Press "Enter" to accept
The full set of options for a program is shown
here; these are shown on the LCD screen three
lines at a time.
DONNA
[7]
Run program
View program
Edit program
Copy program
Delete program
Set password
[
,
to select
]
Use the "Down Arrow" and/or the "Up Arrow"
keys
to select the option you require.
Press the "Enter" key
to select the option.
Edit a selected field or step
Select 'Edit program' and a screen similar to
this will appear.
DONNA
Heated Lid
Preheat Lid
[
,
[
105°C
on
to edit
]
to save
Use the "Down arrow" and/or the "Up arrow"
keys
to select the field or step you want
to edit.
]
You can now edit the active field or step by
first pressing the "Enter" key
27
To edit a temperature, type in the required
temperature. In the example here, the heated lid
temperature is being edited.
DONNA
Heated Lid
105°C
Preheat lid
on
[
edit temperature
]
[
to disable
Press "Enter"
to accept.
Use the "Down Arrow" and/or the "Up Arrow"
keys
to select the next field or step you
wish to edit.
]
DONNA
To edit a field with on/off settings, first select the
field and press "Enter"
Heated Lid
105°C
Preheat lid
on
[
to change
]
Use the "Up Arrow" and "Down Arrow" keys to
toggle
between the settings.
Press "Enter"
[
turn on/off
DONNA
Preheat Lid
Sample cooling
Sample cooling enables the block to be actively
cooled to 4°C whilst the heated lid is reaching
the correct temperature.
on
off
To edit a step with both a temperature and a
time, first press "Enter"
and type in the new
temperature. Press "Enter"
again. Now type
in the new time. The default is minutes.
Pause
off
------------------------In denat 9 4.0°C
00 m30
[ edit temperature]
[
to disable
1. For whole minutes, type the number required
then "Enter" .
]
2. To toggle between minutes and hours, use the
"Decimal Point" key.
Pause
off
------------------------0 0 m30
In denat 94.0°C
[
edit time
]
[
.for hrs/mins
to accept.
]
3. For values less than 1 minute, first press the
"Decimal Point" key followed by the number
of seconds required.
4. For minutes and seconds, type the number of
minutes followed by the "Decimal Point" key
then the number of seconds.
]
Once the values have been added, press "Enter"
to accept.
Once you have finished editing and you are
happy with the changes, press the "End" key
followed by the "Enter" key
to save.
------------------------Fin extn 72.0°C
5m00
Fin hold
10.0°C
[
,
to edit
]
[
to save
If you press the "End" key
a second time for
'No' then all the changes you have made will be
lost.
]
28
To copy a selected program
If you select 'Copy program' a screen similar to
this will appear and the software will
automatically give the program the next
number until it is named.
UNNAMED #
Heated Lid
105°C
Preheat Lid
on
[
,
to edit
]
[
to save
Press "Enter"
and type in a new name. You
will then be able to edit the program as
previously described.
]
To delete a selected program
If you select 'Delete program' a screen similar
to this will appear.
DONNA
Press "Enter"
to delete the program or
"End"
to return to the program screen.
delete program
SURE?
[
Yes or
No
]
To set a password for a selected program
If you want to password protect the program
so that other users cannot edit it, select 'Set
password'. A screen similar to this will appear.
Type in the password you wish to give it and
press "Enter"
. The program list will then
display the key symbol
next to the name to
show that it is locked. Other users will still be
able to copy/view your program but not edit it.
DONNA
New passwd
[
to accept
]
[
]
to exit
OPTION 2: CREATING A NEW PROGRAM
A second option is to create a completely new program without using templates.
A number of typical values are pre-entered
including:
UNNAMED #
Heated Lid
105°C
Preheat Lid
on
Sample cooling
on
Pause
off
------------------------In denat
94.0°C
5m00
Hot Start
off
------------------------Final extn 72.0°C
5m00
Final hold
10.0°C
Heated lid temperature
Preheat lid
Sample cooling
Initial denaturation
Hot start
Final extension
Final hold
These values can be edited and the additional
stages and steps required for cycling can be
added by following the instructions detailed on
the following pages.
29
EXAMPLE PROGRAMS
A couple of example thermal cycling programs are shown below.
Typical 3-Step PCR
3-Step PCR with multiple 'Stages'
Heated Lid
105°C
Preheat Lid
on
Sample cooling
on
Pause
off
------------------------In denat
94.0°C
5m00
Hot Start
off
-------- Stage 1 -------Number cycles
10
Step
94.0°C 0m30
max
Step
60.0°C 0m30
max
Step
72.0°C 0m30
max
-------- Stage 2 -------Number cycles
20
Step
94.0°C 0m30
max
Step
55.0°C 0m30
max
Step
72.0°C 0m30
max
------------------------Final Extn 72.0°C
5m00
Final hold
10.0°C
Heated Lid
105°C
Preheat Lid
on
Sample cooling
on
Pause
off
------------------------In denat 94.0°C
5m00
Hot Start
off
-------- Stage 1 -------Number cycles
30
Step
94.0°C 0m30
max
Step
55.0°C 0m30
max
Step
72.0°C 0m30
max
------------------------Final Extn 72.0°C
5m00
Final hold
10.0°C
PROGRAMMING
Create a new program by selecting ** New
Program ** from the menu and press
"Enter"
* * New Program * * [ ]
2 STEP TEMPLATE 501
3 STEP TEMPLATE 502
[
,
to select
]
Give the program a name and edit any of the
default fields as described above.
UNNAMED #
Heated Lid
105°C
Preheat Lid
on
[
,
to edit
]
[
to save
]
To add a stage and then edit it
Use the "Down Arrow"
key to scroll to
the dotted line below 'Hot Start'; it will start
to flash.
In denat
94.0°C
5m00
Hot Start
off
------------------------[
to insert stage ]
Press "Enter"
30
to insert a stage.
A new stage will be inserted which consists of
a 'Number cycles' field and one step.
-------- Stage 1 -------Number cycles
Step
94.0° C 00m30
max
[
edit no.cycles
]
Edit the number of cycles by typing the
number required then press "Enter"
Use the "Down Arrow"
step.
-------- Stage 1 -------Number cycles
10
Step 94.0° C 00m30
max
[ '.' to insert step
]
[
[
, to edit
to delete step
]
]
key to move to the
Press "Enter"
above.
to edit the step as described
Press "Enter"
again to accept.
To insert additional steps
To insert a new step before the first step, use
the "Up Arrow" or "Down Arrow"
keys to select 'Number cycles'.
-------- Stage 1 -------Number cycles
10
Step 94.0° C 00m30
max
[
'.' to insert step ]
[
,
to edit
Press the "Decimal Point" key to insert a new
step.
]
To insert a new step after an existing step, use
the "Up Arrow" or "Down Arrow"
keys to select the step.
Press the "Decimal Point" key to insert a new
step.
To delete a step
Use the "Up Arrow" or "Down Arrow"
keys to select the step you wish to delete.
-------- Stage 1 -------Number cycles
10
Step 94.0° C 00m30
max
[
'.' to insert step ]
[
[
, to edit
to delete step
Press "Delete"
]
]
The prompt line will flash between 'DEL STEP?'
and 'YES or NO'.
-------- Stage 1 -------Number cycles
10
Step 94.0° C 00m30
max
[
DEL STEP ?
]
[
Yes or
No
to delete the step.
Press "Enter"
to delete the step.
or
Press "End"
]
31
to return to editing the step.
To add a new stage before an existing one
Use the "Up Arrow" or "Down Arrow"
keys to highlight the stage number.
-------- Stage 1 -------Number cycles
10
Step 94.0° C 00m30
max
[
to insert stage ]
[
to delete stage
Press "Enter"
A new stage will be added before the existing
stage. The other stage numbers will be
adjusted as appropriate. The new stage can
now be edited as described above.
]
To add a new stage at the end
Use the "Up Arrow" or "Down Arrow"
keys to highlight the dotted line below the
last stage.
------------------------Final Extn 72.0°C
5m00
Final hold
10.0°C
[
to insert stage ]
Press "Enter"
A new stage will be added with the next
consecutive number. The new stage can now
be edited as described above.
To delete a stage
Use the "Up Arrow" or "Down Arrow"
keys to highlight the stage you wish to delete.
-------- Stage 1 -------Number cycles
10
Step 94.0° C 00m30
max
[
to insert stage ]
[
to delete stage
Press "Delete"
]
The prompt line will flash between 'DEL
STAGE?' and 'YES' or 'NO'.
-------- Stage 1 -------Number cycles
10
Step 94.0° C 00m30
max
[
DEL STAGE?
]
[
Yes or
No
Press "Enter"
]
32
to delete the stage.
To insert a ‘pause’ into a program
To insert a pause after an existing stage,
follow the steps for inserting a stage as
above.
Step 94.0° C 00m30
max
-------- Stage 2 -------Number cycles
Step 94.0° C 00m30
max
[
[
‘.’ to insert step
, to edit
Highlight the 'Number cycles' line then press
"Pause"
]
]
The prompt line will flash between 'CHANGE
TO PAUSE?' and 'YES or NO'.
Step 94.0° C 00m30
max
-------- Stage 2 -------Number cycles
0
Step 94.0° C 00m30
max
[
[
CHANGE TO PAUSE
Yes or
No
Press "Enter"
]
]
The stage number will then be highlighted
with 'Pause' as the only line.
-------- Stage 2 -------Pause
------------------------[
to insert step
]
[
to delete step
to insert a pause.
]
33
TO PROGRAM A GRADIENT
A gradient of temperatures across the block can be useful in optimising the annealing conditions for
primers; a gradient can be set around a temperature in any step of a program. The set temperature is the
temperature required in the middle columns of the block and the gradient is the variation at the two
extremes of the block; the left hand columns being the coolest and the right hand columns the hottest.
A
B
C
D
E
F
G
H
1
2
3
4
5
6
Lowest
temperature
Set
temperature
Gradient
Highest
temperature
The maximum temperature gradient which can be set in the TC-3000G is 15°C; the lowest temperature
is 20°C and the hottest temperature is 80°C. The minimum gradient is 1°C. Examples are given in the
table below:
Lowest temperature
(°C)
Set temperature
(°C)
Highest temperature
(°C)
Gradient
(°C)
20.0
27.5
35.0
15
52.5
60.0
67.5
15
70.0
75.0
80.0
10
52.5
55.0
57.5
5
59.5
60.0
60.5
1
It is important to remember that the greater the gradient, the poorer the uniformity across the block,
therefore fine-tuning may be required to establish the optimum temperature. While the gradient is
mainly linear there are small variations at the extremes of the gradient due to a loss of heat from the
hottest part of the block and a gain of heat from the adjacent column on the coldest. This is the reason
why the first column does not reach the lowest ideal temperature of the gradient and the last column
cannot reach the highest temperature.
34
A gradient calculator is included in the software which can be used to display more accurately the
temperatures of the block for any given gradient. The gradient calculator is located in the menu on the first
screen of the cycler software (see also the section GRADIENT CALCULATOR).
Number cycles
Step 94.0°C 00m30
Step 58.0° C 00m30
[
,
to edit
[
[ '.'
to delete step
to insert step
Use the "Up Arrow" or "Down Arrow"
keys to move to the step where you want to program
a gradient.
30
max
max
]
Press "Enter"
Press "Pause"
]
]
The prompt line will change to flash between
'CHANGE STEP?' and 'YES' or 'NO'.
Number cycles
30
Step 94.0°C 00m30
max
Step 58.0° C 00m30
max
[
CHANGE STEP ?
]
[
Yes or
No
Number cycles
Step 94.0°C 00m30
grad 58.0° C 00m30
[
,
to edit
to go into edit mode.
Press "Enter"
Now press the "Up Arrow" key
]
Using the "Up Arrow" key the line will toggle
between grad, fst (for increment/decrement) and step.
Select grad meaning "gradient".
30
max
max
]
Press "Enter"
twice to go into edit mode.
Use the "Alpha/numeric" keys to change the values
of the grad line to the set temperatures and/or times
you require.
Number cycles
30
Step 94.0°C 00m30
max
grad 58.0° C 00m30
max
[
edit temperature
]
Press "Enter" after each field you change. You will
exit 'Edit Mode' when you reach the end of the line.
Use the "Down Arrow" key
'gradient' line.
Step 94.0°C 00m30
max
Step 58.0° C 00m30
max
gradient
5.0°C
[
edit temperature
]
to move onto the
Press "Enter" to edit the gradient range. A gradient
range between 1 and 15°C can be entered.
Press "Enter"
to accept.
If the step temperature is edited such that, for the
currently entered gradient range, the unit will be
forced outside the gradient limits, the gradient range
will be reduced so as to preserve the set temperature.
Step 94.0°C 00m30
max
Step 75.0° C 00m30
max
gradient
15.0°C
[
edit temperature
]
35
A warning prompt will be given on the screen.
Step 94.0°C 00m30
max
Step 75.0° C 00m30
max
gradient
10.0°C
[
gradient reduced
]
If the gradient range is edited such that, for the
currently entered step temperature, the unit will be
forced outside the gradient limits, the step
temperature will be reduced so as to preserve the
gradient range.
Step 94.0°C 00m30
max
Step 75.0° C 00m30
max
gradient
15.0°C
[
edit temperature
]
A warning prompt will be given on the screen
showing whether the temperature has been reduced
or increased.
Step 94.0°C 00m30
max
Step 72.5° C 00m30
max
gradient
15.0°C
[
temp reduced
]
Cycle 7/30
Sample 55.5°C ➔
Hold @ 58.0°C
[
to STOP
[
[
to pause
for info
When the program is running, the screen will indicate
the temperature range of the columns in the gradient
step.
56m08
60.5°C
00m17
]
]
]
36
TO PROGRAM INCREMENT OR DECREMENT TEMPERATURE OR TIME
Under normal circumstances, the Hold Temperature of all steps is constant. However, it is possible to
automatically increment or decrement the temperature of a specified step of a programme.
1
1
1
1
1
1
3
Temperature
3
2
Cycle 1
O+∂O
3
O
3
O+4∂O
O+3∂O
O+2∂O
3
2
2
2
2
Cycle 2
Cycle 3
Cycle 4
Cycle 5
Temperature profile of incremented Hold Temperature at step 3 of each cycle
The Hold Temperature of the incremented/decremented step is the defined Hold Temperature plus the
summation of the increments/decrements. The first cycle is never incremented/decremented, only
subsequent cycles. If you select a decrement, the Hold Temperature is prevented from falling below 4°C.
If you select an increment, the Hold Temperature is prevented from rising above 99°C.
Similarly, under normal circumstances, the Hold Time of all steps is constant. However, it is possible to
automatically increment or decrement the duration of a specified step of a program.
1
1
Temperature
1
3
2
Cycle 1
2
t+∂t
Cycle 2
Cycle 3
3
3
2
2
t+2∂t
1
1
3
3
2
t
1
t+3∂t
Cycle 4
t+4∂t
Cycle 5
Temperature profile of incremented Hold Time at step 3 of each cycle
The Hold Time of the incremented/decremented step is the defined Hold Time plus the summation of the
increments/decrements. The first cycle is never incremented/decremented, only subsequent cycles. If you
select a decrement, the Hold Time is prevented from falling below one second.
37
Number cycles
Step 94.0° C 00m30
Step 58.0° C 00m30
[
, to edit
[
[
to delete step
‘.’ to insert step
Number cycles
Step 94.0° C 00m30
Step 58.0° C 00m30
[
CHANGE STEP ?
[
Yes or
Number cycles
Step 94.0° C 00m30
fst 55.0° C 00m30
[
,
to edit
30
max
max
]
to go into edit mode.
Press "Pause"
The prompt line will change to flash between
'CHANGE STEP?' and 'YES' or 'NO'.
30
max
max
]
]
to save
Press "Enter"
]
]
No
[
Use the "Up Arrow" or "Down" Arrow"
keys to move to the step where you want an
increment or decrement temperature or time
30
max
max
]
Press "Enter"
Now press the "Up Arrow" key
Using the "Up Arrow" key
the line will
toggle between grad, fst and step. Select
"fst" meaning "first".
Press "Enter"
twice to go into edit mode.
]
Use the "Alpha/numeric" keys to change the
values of the 'fst' line to the first
temperatures and/or times you require.
Number cycles
30
Step 94.0° C 00m30
max
fst
65°C 00m30
max
[
edit temperature
]
Press "Enter"
after each field you change.
You will exit 'Edit Mode' when you reach the
end of the line.
Step
fst
lst
[
[
[
94.0° C 00m30
65.0° C 00m30
55.0° C 00m30
,
to edit
to delete step
‘.’ to insert step
Use the "Down Arrow" key
the "lst" line, meaning "last".
max
max
Press "Enter"
]
]
]
38
to move onto
to edit the step.
Step
fst
lst
[
94.0° C 00m30
65.0° C 00m30
55.0° C 1m30
edit time
max
max
[
.For hrs/mins
]
Use the "Alpha/numeric" keys to change the
values of the 'lst' line to the final
temperatures and/or times you require.
Press "Enter"
]
for each field you change.
You will exit 'Edit Mode' when you reach the
end of the line.
When you run the program it will automatically increment or decrement in equal steps from the first time
and/or temperature to the last over the number of cycles in the stage. For example: if the first
temperature is 65°C and the last temperature is 55°C, over 30 cycles the temperature will decrease
0.33°C each cycle. Similarly, in the example shown, the time would increase from 30 seconds to 1 minute
30 seconds in 2 second increments.
To save the program
A program can only be run once it has been
saved. Press the "End"
key.
-------- Stage 1 -------Number cycles
10
Step 94.0° C 00m30
max
[
SAVE?
]
[
Yes or
No
The prompt line will flash between 'SAVE?'
and 'YES or NO'.
Press "Enter"
for 'Yes' to save the changes
you have made.
]
If you press "End"
for 'No' then all the
changes you have made will be lost.
The program is now ready to run and will be
allocated with a unique "Speed dial" number
which can be used to rapidly locate the
program from the main start-up screen.
GEORGE
(10)
Run Program
View Program
[
,
to select
]
Use the "Up Arrow" and "Down Arrow"
keys to scroll through the list of options.
Stopping what you are doing
At any time during programming the TC-3000G, when you are satisfied with the program, you can finish
and accept what you have done by first pressing the "End" key, then follow the instructions on screen.
If you are not sure, wait for the instructions in the prompt line to scroll back to the first line that you see.
39
TO RUN A PROGRAM
When the unit is switched on the display will
show this screen.
Programs
[0]
Information
Configuration
[
,
to select
]
If known, type in the 'Speed dial' number for
the required program and press "Enter"
This will take you directly to the required
program.
Programs
[7]
Information
Configuration
[
,
to select
]
If you do not know the 'Speed dial' number,
press "Enter"
to access the list of stored
programs.
* * New Program * * [ ]
2 STEP TEMPLATE 501
3 STEP TEMPLATE 502
[
,
to select
]
DAVID
DONNA
[
Next either:
1. Scroll down to the required program using
the "Up Arrow" and "Down Arrow"
keys.
2. Or type the first letter of the program name
in the "New program" field; this will then
list all the program names beginning with
that letter. Use the "Up Arrow" and
"Down Arrow" keys
to select the
required program.
8 [D]
7
,
to select
]
Press "Enter" to accept
'Donna'.
For example
To run the program, select 'Run program' and
press "Enter"
DONNA
[7]
Run Program
View Program
[
,
to select
]
A screen similar to this one will appear.
DONNA
[
[
Press "Enter"
RUN PROGRAM?
Yes or
No
]
]
40
to start the run.
Cycle 4/30
Sample 58.0°C
Hold @ 58.0°C
[
to STOP
[
[
During the run the LCD screen will report:
56m08
1. The cycle number
00m17
]
to PAUSE
for info
2. The time remaining (counting down in
seconds)
3. The sample temperature (whether holding
or ramping)
]
]
4. The current step of the run
5. A count down of the hold time.
An example is shown here.
Manual pause
Cycle 24/30
Sample 72.0°C
Hold @ 72.0°C
[
PAUSE?
[
Yes or
To pause a program while it is running, press
"Pause"
The run will continue but the
prompt line will alternate 'PAUSE?' and 'YES
or NO'
19m26
00m10
]
No
To confirm that you want the program to
pause press "Enter"
]
If you have pressed the "Pause" key by
mistake and you want the program to
continue, press "End"
Cycle 24/30
Sample 72.0°C
Paused
[
to STOP
[
[
The unit will emit a beeping sound while
paused.
19m26
To resume the run, press "Pause"
followed by "Enter"
to continue.
]
to CONTINUE
for info
]
]
Manual stop
To stop a program while it is running, press
"End"
Cycle 24/30
10m39
Sample 94.0°C
Hold @ 94.0°C
00m10
[
STOP PROGRAM?
]
[
Yes or
No
The run will continue but the prompt line will
alternate 'STOP PROGRAM?' and 'YES or NO'
To confirm that you want to stop the program
press "Enter"
]
If you have pressed the "End"
key by
mistake and you want the program to
continue, press "End"
again.
When a program is stopped the unit will emit
a beeping sound and the original program
screen will appear.
41
Program Information
Pressing "Enter"
while a program is
running will give information about the
current run including which program is
actually running and the unit status e.g.
holding or ramping.
DONNA
Heated Lid
on
Status
ramping
[
to exit
]
An example is shown here.
INFORMATION
The Information field on the front screen
contains all the unit-specific information such
as serial number and software version.
Programs
[0]
Information
Configuration
[
,
to select
]
Block
Block s/n
Unit s/n
S/w version
H/w version
Comms address
Unit cycles
Block cycles
Press "Enter"
information.
to access the unit
The complete list is displayed here.
48x0.2ml
139160-2
GP0000-01
14.zz
01
1
624
69
Note that the "Comms address" must be
unique for each unit if more than one cycler is
linked to a PC using the Gensoft control
software.
CONFIGURATION
Warning! The Configuration software should only be accessed by designated personnel, as some
parameters can be changed or may be lost when using it.
The Configuration field on the front screen
allows a system administrator access to set up
all the default settings for the programs, time
and date settings and to change and remove
passwords.
Programs
[0]
Information
Configuration
[
,
to select
]
Access to the 'Configuration' set up is
password protected.
CONFIGURATION
Password
[
pad
to accept
[
to exit
To enter configuration, use the alpha keys to
type in 'pad'
]
Press "Enter"
]
42
There are a number of options which can be
changed; these are listed here.
Program defaults
Settings
Passwords
Controller reset
Use the "Up Arrow" and "Down Arrow"
keys to select then press "Enter"
to access
any of the options. Each option is discussed in
detail below.
Program defaults
Highlight 'Program defaults' then press
"Enter"
Program defaults
Settings
Passwords
Controller reset
The complete list of program defaults is given
here. These are the values that all new
programs will take when you start to create a
new program.
Heated lid
105°C
Preheat lid
on
Sample cooling
on
Pause
off
------------------------In denat
94.0°C
5m00
Hot start
off
------------------------Final extn 72.0°C
5m00
Final hold
10.0°C
[
,
to select
]
To edit any line use the "Up Arrow" or
"Down Arrow" keys
to select the line
you wish to change.
Press "Enter"
then change the value using
the numerical keys. Press "Enter"
to accept
the new value.
To edit a field with on/off settings, first select
the field and press "Enter"
Use the "Up Arrow" and "Down Arrow"
keys to toggle between the settings. Press
"Enter"
to accept.
When you have set all the defaults, press the
"Enter"
key to go back to the
Configuration menu.
Note: altering the default values does not
change existing programs.
Settings
Highlight 'Settings' then press "Enter"
Program defaults
Settings
Passwords
Controller reset
43
Set date
xx/xx/xx
Set time
xx:xx
Date format
dmy
Auto restart
on
Beeper
on
Coms address
1
Coms speed
4800
[
,
to select
]
The complete list of settings is given here.
Auto restart "on" will allow the unit to restart
from where it left off should there be a power
failure and the power returns.
The "Comms address" identifies individual units
and must be unique if there are several units linked
to each other.
The optimum "Coms speed" is set at 4800. When
using the Gensoft control software, ensure all
cyclers are set to the same value.
Edit any line as described for 'Program defaults'
above. Press "Enter"
to accept the new value.
When you have finished, press the "Enter"
to go back to the 'Configuration' menu.
key
Passwords
The 'Passwords' function allows the administrator
to change the 'Admin password' (which allows
access to Configuration) and also to delete any
passwords associated with programs.
Program defaults
Settings
Passwords
Controller reset
Highlight 'Passwords' then press "Enter"
* * Admin passwd * *
DONNA
GEORGE
[
,
to select
]
The screen will appear as opposite.
We suggest that you change the 'Admin password'
at this stage in order to protect the configuration
settings.
Use the "Up Arrow"
passwd
ADMIN PASSWD
Password
Confirm
[
to accept
]
key to highlight 'Admin
'Press "Enter"
and type in the new password.
Repeat to confirm and accept.
The screen will go back to the 'Configuration'
menu.
* * Admin passwd *
DONNA
GEORGE
[
DEL PASSWORD?
To delete a password associated with a program,
use the "Up Arrow" and "Down Arrow"
keys to highlight the program.
*
]
Press "Enter"
Press "Enter"
[
Yes or
No
]
44
again to delete the password.
Controller reset
The 'Controller reset' will delete all saved
programs from the unit.
Program defaults
Settings
Passwords
Controller reset
Highlight 'Controller reset' then press
"Enter"
CONTROLLER RESET
This will delete
all programs
[
SURE ?
]
[
]
Yes or
No
To delete all the programs, press "Enter"
45
GRADIENT CALCULATOR
It is not possible to view the actual temperatures of each column in the progam or while the unit is
running. Therefore for any set gradient, the gradient calculator can be used to calculate the temperatures
for each of the columns and display them on the screen. This allows the user to determine the optimal
amplification conditions for the assay.
The gradient calculator field on the front
screen allows a user to input a temperature
and gradient range. It then calculates the
temperatures for each of the columns.
Programs
[0]
Information
Configuration
Gradient calculator
[
,
to select
]
From the front screen scroll down to Gradient
calculator.
Gradient calculator
Temp 50.0°C
Grad 5.0°C
Column 1
47.5°C
[
,
to select
]
Press "Enter"
Press "Enter" again to edit the
temperature and gradient range.
Gradient calculator
Temp 55.0°C
Grad 5.0°C
Column 1
47.5°C
[
edit temperature
]
Press "Enter" when finished and use the
"Up Arrow" and "Down Arrow"
keys to scroll through the list of column
temperatures.
Gradient calculator
Temp 55.0°C
Grad 10.0°C
Column 1
50.5°C
Column 2
51.6°C
Column 3
53.2°C
Column 4
55.2°C
Column 5
56.9°C
Column 6
58.2°C
Column 7
59.1°C
Column 8
59.6°C
[
,
to select
]
46
RUNNING THE TC-3000G FROM A PC
The TC-3000G can be run from a PC using Bibby Scientific Ltd's Gensoft software. Instructions for using
Gensoft are included in a separate manual.
Connecting one unit to a PC
To connect a single TC-3000G unit, connect the unit to the PC using the USB cable, part number
6205046.
TC-3000G
USB cable
Connecting several TC-3000G units to a PC.
If you are driving two or more units then you will need a USB cable for each unit, part number 6205046
plus a hub, which will connect multiple units together.
Computer
USB
cable
USB hub
USB cables
47
NOTES ON TRANSFERRING PROTOCOLS FROM OTHER THERMAL CYCLERS
The TC-3000G can heat up to 3.3°C/s and provides the highest levels of temperature uniformity. Many
slower machines have historically begun the countdown on hold times before the set temperature is
reached in order to avoid any overshooting in sample temperature. The slower the heating rate the more
significant the loss in hold time, i.e. if a 30 second hold time is set at 55°C then perhaps 5 to 10s will be
lost in between the start of the time countdown and actually reaching the set temperature. The overall
effect of this method of control is that your sample, if cooling from a higher temperature, is subjected to
an environment with an elevated temperature compared to the target temperature for a significant part
of the hold time.
The TC-3000G provides the user with 'what-you-program-is what-you-get'. Due to the rapid and
responsive nature of the Peltier heating system, the time between the timer countdown initiating and
actually reaching the set temperature has been reduced to as little as 1s. This ensures that the sample is
only subjected to the exact temperature required.
Taking the above into consideration it must therefore be noted that care must be taken when reoptimizing protocols from a slower machine.
Trouble Shooting
1. The optimum annealing temperature may in fact be higher than the set temperature used in the slower
machine. Try increasing it by 1 or 2°C.
2. The hold time can be reduced once the optimum temperature has been found. Try reducing it by 5 to
10s.
48
USER MAINTENANCE
CLEANING YOUR TC-3000G
Before cleaning your unit, disconnect from the power supply and allow it to cool to below 50°C.
CLEANING YOUR BLOCK
The heating/cooling block, including wells and flat surfaces, should be cleaned regularly to ensure
optimum heat transfer to the samples. Always clean the block if there has been a spillage. Use a cloth or
cotton buds dipped in a fresh, 50:50 water/isopropanol solution and make sure that no deposits are left
in the wells.
In the case of radioactive spillages the heater block can be removed from the unit for more detailed
cleaning. Bibby Scientific Ltd recommends that you use a proprietary cleaning agent and follow the
manufacturer’s instructions. The heating/cooling block is made of aluminium, therefore an agent such as
Neutracon (from Decon Labs Ltd.), suitable for nonferrous metals should be used. However, remember
that other parts of the unit are made of ferrous materials and may be damaged by spillage onto them.
HEATER BLOCK REMOVAL
Removing the block may expose the possibility of electric shock; disconnect the unit from the
mains and allow to cool below 50ºC before continuing. Remove the four screws on the underside
of the unit and drop the block/fan assembly down. Unplug the assembly from the wiring and remove the
assembly from the unit. Refitting the block/fan assembly is the reverse procedure; be careful of the wires
as you refit the block.
Underside of the TC-3000G
Four screws which hold the
block/fan assembly in place
CLEANING YOUR CASE
The outer case of the TC-3000G can be cleaned with a cloth dipped in water or ethanol (50:50
methanol/water or 99:1 water/formaldehyde can also be used). No part of the case or cover should be
immersed in the solvents. Do not use aggressive solvents such as acetone, or abrasive cleaners. Before
using any cleaning or decontamination method except those recommended here, the responsible person
should check with Bibby Scientific Ltd that the proposed method will not damage the equipment.
49
SOME FREQUENTLY ASKED QUESTIONS
Q1 What is the 'Pause' function at the start of the program used for?
A1 Some users prefer to preheat the heated lid before placing the samples into the unit. The pause
feature is used to stop the unit after the 4 minute heated lid preheat step. It will also sound an
audible alarm indicating that the machine is ready for the sample tubes or plate to be added.
Pressing the "Pause" key followed by the "Enter" key will commence the remaining program.
Q2 Why do you have to press some keys twice to make the unit do what you want it to do?
A2 With many of the program features there is a fail-safe function which asks if you are sure you want
to perform a particular action. You are required to press one key to select a function followed by a
second to accept it.
Q3 What is 'Hot Start'?
A3 A "hot start" is used for reducing non-specific product formation during the first cycle of
amplification. The 'Hot Start' programming step is used to pause the instrument at a specific
temperature, typically around 70ºC, after the initial template denaturation. The reason is to allow
the manual addition of unmodified Taq DNA polymerase which may loose activity if added during
the initial 5min denaturation. Heat-activated Taq or Hot Start enzymes do not require this step.
Q4 What is the incremental function?
A4 Incremental timing and temperature are used to increase or decrease either the time or temperature
incrementally over the number of cycles in a stage.
Incrementation of extension time is used with 'Long PCR' which is when large template fragments
are to be amplified (e.g. 27kb lambda DNA, 40kb genomic DNA).
Decremental temperature is used for protocols such as 'Touchdown PCR' where one starts with a
high annealing temperature in the first cycle and gradually decreases the temperature over the
number of cycles in the stage. This ensures that only the specific product is amplified.
Q5 The heat-up ramp-rate in the specification is 3.3°C/second yet I can only manually set it to
2.0°C/second. Why is this?
A5 If you set the ramp-rate to max the block will heat at 3.3°C/second the unit sets its own control
parameters. If you program a ramp-rate the control parameters reduce the ramp-rate. This is a
feature of the software and not the actual ramp-rate.
If you require further technical or applicational assistance please contact Bibby Scientific Ltd at:
[email protected]
For servicing information please contact: [email protected]
We are continually striving to improve our thermal cyclers and software. If you have any comments and
suggestions on how we can do things better please send them to us at:
[email protected]
50
ADDITIONAL INFORMATION
Brief fault finding notes and a list of replacement parts are given in this section.
Note that this equipment should only be dismantled by properly trained personnel. Removing the outer
cover exposes potentially lethal mains voltages. There are no user serviceable parts within this equipment.
FAULT FINDING
Should you have any problems with your unit which cannot be easily remedied, you should contact your
supplier and return the unit if necessary. Please include details of the fault observed and remember to
return the unit in its original packing. Bibby Scientific Ltd accepts no responsibility for damage to units
which are not properly packed for shipping: if in doubt, contact your supplier, giving the full serial number
of the unit and software version number (shown when the unit is first switched on).
FUSES
If neither the power light nor display on the front panel is lit, one of the two fuses may have blown.
Check that there is no external cause, such as a faulty plug or lead. Check both fuses and replace the
faulty fuse with a new one of the correct value (fuse values are given on the label next to the power inlet).
Note that fuses should only be replaced by a qualified electrician.
The holder for the two fuses is built into the mains input socket. First remove the power cable and then
gently prise the fuse drawer open with a flat-bladed screwdriver or similar tool.
Each fuse can be removed by using the screwdriver as a lever.
Exchange both fuses in the fuse holder for a working fuse of the correct value. Finally, replace the fuse
drawer in the fuse compartment and push the drawer shut.
Fuses which blow repeatedly indicate a serious fault and you should return the unit to your supplier for
repair.
THE HEATED LID OVER-TEMPERATURE CUT-OUT
The heated lid is fitted with an independent circuit to protect it from overheating. In the unlikely event
of an over-temperature problem with the lid, the unit is fitted with a thermal fuse which will remove
power to the heater plate should the maximum temperature be exceeded. If this fault occurs, contact
your local supplier for repair.
INSULATION TESTING
This equipment is fitted with RFI suppression circuitry. Any check of the electrical insulation by means of
high voltage dielectric testing (for example as in BS EN 61010-1) must be carried out using only a DC
voltage.
This unit contains semiconductor components which may be damaged by electric field effects.
INTERCHANGEABLE BLOCKS
The block can be removed and replaced by another of the same sort or of a different sort. The software
will register the change and set up the unit for the new block.
Remove the four screws on the underside of the unit and drop the block/fan assembly down. Unplug the
assembly from the wiring and remove the assembly from the unit. Refitting the block/fan assembly is the
reverse procedure; be careful of the wires as you refit the block.
51
Underside of the TC-3000G
Four screws which hold the
block/fan assembly in place
ACCESSORIES
The following accessories can be obtained from Bibby Scientific Ltd or your Bibby Scientific Ltd dealer:
Item No
Description
FTC3G/02/B
0.2ml Tube block for TC-3000G
FTC3G/05/B
0.5ml Tube block for TC-3000G
FTUB02TW
0.2ml Micro tubes
Quantity
1
1
Pack of 1000
FTUB05TW
0.5ml Micro tubes thin wall
FSTRIP2
Strips of 0.2ml tubes for use with flat or domed caps
Pack of 125 strips
Pack of 1000
FSTRIP3
Strips of 0.2ml tubes, each tube has individual flat cap
Pack of 120 strips
FSTRIP4
Strips of 0.2ml tubes with attached, hinged strip of domed caps Pack of 125 strips
F8CAP2
Strips of 8 domed caps for use with FSTRIP2
Pack of 125 strips
F8CAP3
Strips of 8 flat caps for use with FSTRIP2
Pack of 125 strips
FCOOL
Mini cooler. Suitable for all 0.2ml tubes, strip tubes & plates
F24PLNS
24 well plate, non-skirted
40 plates
F48PLNS
48 well plate, non-skirted
20 plates
F24MAT
24 well sealing mat (silicone) for use with F24PLNS
10 mats
F48MAT
48 well sealing mat (silicone) for use with F48PLNS
10 mats
52
1
REPLACEMENT PARTS
The following replacement parts can be obtained from Bibby Scientific Ltd or your Bibby Scientific Ltd
dealer:
Item No
Description
FCABRTUK
Mains cable and plug, 230V UK
Quantity required
1
FCABRTEU
Mains cable and Schuko plug, 230V Europe
1
FCABRTUS
Mains cable and 3-pin plug, 100V/120V US type
1
6500342
Fuse T6.3A
2
6106196
Heated lid mat
1
53
Bibby Scientific France SAS
Bibby Scientific Italia Srl
ZI du Rocher Vert - BP 79
77793 Nemours Cedex
France
Tel: +33 1 64 45 13 13
Fax: +33 1 64 45 13 00
e-mail: [email protected]
www.bibby-scientific.com
Via Alcide de Gasperi 56
20077 Riozzo di Cerro al Lambro
Milano Italia
Tel: +39 (0)2 98230679
Fax: +39 (0)2 98230211
e-mail: [email protected]
www.bibby-scientific.it
Bibby Scientific Ltd
Techne Inc.
Bibby Scientific (Asia) Ltd
Beacon Road Stone
Staffordshire ST15 0SA
United Kingdom
Tel: +44 (0)1785 812121
Fax: +44 (0)1785 813748
email: [email protected]
www.bibby-scientific.com
3 Terri Lane Suite 10
Burlington NJ 08016
USA
Tel: 800-225-9243
Fax: 609-589-2571
email: [email protected]
www.techneusa.com
Room 607 Yen Sheng Centre
64 Hoi Yuen Road Kwun Tong
Kowloon Hong Kong
Tel: +852 3583 1581
Fax: +852 3583 1580
e-mail [email protected]
www.bibby-scientificasia.com
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