Thermo Scientific TSQ Endura Triple-Stage Quadrupole Mass Spectrometer

Thermo Scientific TSQ Endura Triple-Stage Quadrupole Mass Spectrometer
Extreme quantitative value with unprecedented ease
RFQ Product Specif ications
Thermo Scientific TSQ Endura
Triple-Stage Quadrupole
Mass Spectrometer
• Extreme quantitative value in a triple quadrupole
mass spectrometer
• Best-in-class sensitivity
• Ultrafast selected-reaction monitoring enables
quantification of more compounds in less time
• Superb robustness and reliability
• Close integration with application-specific software
ensures maximum productivity
• Simple method development and easy operation
The Thermo Scientific™ TSQ Endura™
triple-stage quadrupole mass spectrometer
provides unparalleled value, with LODs and
LOQs unrivaled in its class. It delivers this
best-in-class quantitation run after run and
day after day, even for complex and
difficult-to-run samples. Close integration
with application-specific software ensures
maximum productivity. The instrument’s
excellent analytical performance doesn’t
come at the price of complexity or lack of
durability; breakthrough software and hardware
developments make operation far easier and
more reliable than in previous-generation
triple quadrupoles. The TSQ Endura MS
helps users spend more time thinking about
their analyses and less time worrying about
instrument setup and operation.
Hardware Features
Thermo Scientific™ EASY-Max NG™
API Source
• Automatic connection of all gases and
voltages on installation for simpler
operation and greater reliability
• Automatic source recognition for ease of
use and simplified data logging
• Sweep gas reduces chemical noise
• Enhanced exhaust port efficiently removes
solvent vapor, improving uptime and
reducing chemical noise
• Optimal 60 degree spray angle
• Minimal adjustment
• Interchangeable HESI and APCI
ionization probes
• Dual-mode HESI and APCI capability
Ion Optics
The progressively spaced stacked-ring ion
guide (RF-Lens) captures and efficiently
focuses ions in a tight beam. Large variable
spacing between electrodes provides better
pumping efficiency and improved
ruggedness. Automatic tuning program
optimizes ion transmission.
Ion Beam Guide
The ion beam guide, with its neutral blocker,
stops neutrals and high-velocity clusters,
keeping the ion optics cleaner, reducing
noise, and increasing sensitivity.
Hyperbolic Quadrupole Mass Filter
(Q1 and Q3)
Hyperbolic quadrupoles with high ion
transmission at isolation widths down to
0.4 amu provide excellent sensitivity and
Active Collision Cell (Q2)
The high-pressure argon-filled collision cell
produces efficient fragmentation for high
sensitivity. An axial DC field speeds ion
transits, yielding up to 500 SRMs/s with
zero cross talk.
Figure 1. TSQ Endura ion optics
Vacuum System
• Four-stage differentially pumped vacuum
• Advanced triple-inlet turbomolecular pump
integrated with the vacuum manifold
• Single rotary vacuum pump configuration
• Dual-mode discrete-dynode detector
increases sensitivity by operating in
pulse-counting mode when ion flux is low
and analog mode when ion flux is high
• Six orders of dynamic range provide
high-confidence quantitation
Integrated Divert Valve and Syringe Pump
Fully automated data system control of the
divert valve enables switching the solvent
front, gradient end point, or any portion of
the HPLC run to waste.
Optional Ion Sources
• Thermo Scientific™ Ion Max NG™ ion
source provides ultimate performance with
full adjustments for optimization
• Thermo Scientific™ EASY-Spray NG™ ion
source is designed for maximum
nanoelectrospray performance with no
need for adjustments
• T hermo Scientific™ Nanospray Flex NG™
ion source is designed for ultimate
nanoelectrospray performance with
complete flexibility of column selection
Active collision cell
with axial DC fields
Hyperbolic quadrupole
mass filter
Ion beam guide with
neutral blocker
mass filter
EASY-Max NG ion source
Software Features
Performance Specifications
Installation Requirements
Data System
• High-performance PC with Intel®
• High-resolution LCD color monitor
• Microsoft® Windows® 7 operating
system (64 bit)
• Microsoft Office 2010 software
• Three 230 Vac ±10%, 50/60 Hz at 16 A
• Four 120 Vac +6–10%, 50/60 Hz at 20 A
or four 230 Vac ±10%, 50/60 Hz at 13 A
• Earth ground hardwired to main panel
• Free from voltage variations above or
below the recommended operating range
Standard MS Software
• Thermo Scientific™ Xcalibur™ processing
and instrument control software
• Tune editor for system calibration,
diagnostics, and manual data acquisition
• Method editor with comprehensive
application-specific template library and
drag-and-drop user interface to facilitate
method development
• Automated optimization of all instrument
parameters, including gas pressures and
collision energy, within an experiment
• Direct control of multiple vendors’ LC
systems and autosampler configurations
through Xcalibur software
• Foundation of shared, state-of-the-art
software makes it easier to transfer methods
between next-generation instruments
Scan Functions
• Highly sensitive full-scan MS in Q1 or Q3
• Selected-ion monitoring (SIM) in Q1 or Q3
• Selected-reaction monitoring (SRM) with
up to 30,000 SRMs definable and up to
500 SRMs/s
• High-resolution (0.4 Da) selected-reaction
• Product ion scanning
• Precursor ion scanning
• Neutral-loss scanning
• Reverse energy ramp (RER) MS/MS
spectra gives information-rich MS/MS
spectra for solid compound identification
• Polarity switching capabilities
Optional Application-Specific Software
• Thermo Scientific™ LCQUAN™ quantitation
software supports 21 CFR Part 11
• Thermo Scientific™ TraceFinder™ software
simplifies method development and routine
analysis in food safety, environmental,
clinical research, and forensic toxicology
• Thermo Scientific™ Pinpoint™ software
automates peptide quantitation applications
• Thermo Scientific™ Mass Frontier™
spectral interpretation and classification
software for the identification of unknowns
Positive Electrospray (HESI) at
Resolution 0.4 Da FWHM
A 2 µL injection of a 500 fg/µL reserpine
solution on a Thermo Scientific™ Hypersil
GOLD™ aQ 20 x 2.1 mm 1.9 µm particle
packed column at a flow rate of 300 µL/min
30:70:0.02 water/methanol/formic acid will
produce a minimum signal-to-noise ratio of
10,000:1 for the transition of the protonated
molecule at m/z 609.3 to the fragment ion at
m/z 195.1 when operated in selectedreaction monitoring (SRM) mode with Q1
and Q3 resolution set to 0.4 and 0.7 Da
FWHM respectively.
Mass Range
m/z 10-3400
Q1 and Q3 adjustable to 0.4 Da peak width
(FWHM) across the entire mass range
Scan Rate
15,000 amu/second at a resolution of 2 FWHM
500 SRMs/second (for any resolution from
0.4 through 2.0 FWHM)
Mass Stability
Mass assignment will be within ±0.05 Da
over a 24 hour period. The laboratory room
temperature must be maintained between
18–27 °C (65–81 °F). The room
temperature may not change by more than
5 °C (9 °F) during this period.
• Collision gas: 99.995% pure argon
ollision gas supply pressure: 135 ± 70 kPa
(20 ± 10 psig)
• S heath/aux/sweep gas: 99% pure nitrogen
• Sheath/aux/sweep gas supply pressure:
690 ± 140 kPa (100 ± 20 psig)
• Maximum sheath gas consumption:
≈20 L/min
• Functional temperature range: 15–27 °C
(59–81 °F)
• Optimal temperature range: 18–21 °C
(65–70 °F)
• Heat output: 1550 W (5400 Btu/h)
• Total system heat output: 4420 W
(15,380 Btu/h)
• Particulate matter: <3,500,000 particles
per cubic meter of air (<100,000 particles
of >5 µm diameter per cubic foot of air)
• Relative humidity: 20% to 80%, without
• Floors must be free of vibration
• TSQ Endura MS1: 680 × 760 × 840 mm
(h, w, d – 27 × 30 × 33 in)
• Oerlikon® SV 65 forepump: 270 × 320 ×
489 mm (h, w, d – 10 × 13 × 19 in)
• TSQ Endura MS: 125 kg (275 lb)
• Oerlikon® SV 65 forepump: 52 kg (115 lb)
1. Includes the divert/inject valve
RFQ Product Specif ications
For Research Use Only. Not for use in diagnostic procedures.
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PS63846_E 07/13M
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