Model V15�17 Vertical Gel Electrophoresis Apparatus User Manual

Model V15�17 Vertical Gel Electrophoresis Apparatus User Manual

Model V15•17 Vertical Gel

Electrophoresis Apparatus

CAT. SERIES 21080

Table of Contents

1. Notices to Customer

........................................................................................ 1

1.1 Important Information ................................................................................. 1

1.2 Warnings .................................................................................................... 1

2. Overview

........................................................................................................... 2

3. Operating Instructions

.................................................................................... 3

3.1 Gel Casting ................................................................................................. 3

3.1.1

Preparing the Gel Plate Assembly Using the Bottom Spacer ......... 3

3.1.2

3.1.3

Preparing the Gel Plate Assembly Using the V16 Casting Clamp ...4

Pouring Discontinuous Buffer System Gels .................................... 5

3.1.4

Pouring Continuous Buffer System Gels ........................................ 5

3.2 Assembling the Gel Electrophoresis Apparatus ......................................... 5

3.3 Loading Samples ........................................................................................ 6

3.4 Electrophoresis ........................................................................................... 7

3.5 Post-Electrophoresis .................................................................................. 7

4. Troubleshooting Guide

.................................................................................... 8

5. Related Products

.............................................................................................. 9

6. Additional Information

................................................................................... 11

6.1 Care and Handling.................................................................................... 11

6.2 Maintenance ............................................................................................. 11

6.3 Specifications ........................................................................................... 12

6.4 Warranty ................................................................................................... 12

6.5 Declaration of Conformity and

CE

Mark ....................................................12

Figures

1. V15•17 Vertical Gel Electrophoresis Apparatus................................................. 2

2. Assembly of Side and Bottom Spacers .............................................................. 3

3. Assembly Using the V16 Casting Clamp ............................................................4

4. Cross Section of Banana Plug Assembly..........................................................10

Tables

1. Gel Solution Volumes as a Function of Gel Thickness ...................................... 5

2. Sample Loading Volumes for V15•17 Apparatus Combs as a Function of Gel Thickness .......................................................................... 6

B

ENCH

M

ARK

, T

ECH

-L

INE

SM , and the Life Technologies logo are marks of Life Technologies, Inc.

Ficoll ® is a registered trademark of Amersham Pharmacia Biotech A.B.

Teflon ® is a registered trademark of E.I. duPont de Nemours & Co.

C

OOMASSIE

B

RILLANT

B

LUE

R ® is a registered trademark of Imperial Chemical Industries PLC.

i

Notices to Customer

!

1.1

Important Information

This product is authorized for laboratory research use only. The product has not been qualified or found safe and effective for any human or animal diagnostic or therapeutic application. Uses for other than the labeled intended use may be a violation of applicable law.

If the product is used in a manner not specified by the manufacturer, the protection provided by the product may be impaired.

1.2

1.

Warnings

DANGER! SHOCK HAZARD!

This apparatus requires a 400-V DC power supply for operation and is, therefore, a potential shock hazard. The power supply should have open-circuit sensing. This apparatus should always be operated with caution. Careless handling can result in electrical shock. The system should be operated by trained personnel only. This manual should be readily accessible to all users.

2. Never operate damaged or leaking equipment.

3. Always turn off the DC power source prior to disconnecting the power cords from the apparatus.

4. Inspect the electrophoresis apparatus, all electrical connections, and power cords prior to operating this equipment. See section 6.2 for additional information.

5. For maximum safety, always operate this apparatus in an isolated, low traffic area that is not accessible to unauthorized personnel.

6. Certain reagents indicated for use in this manual are of a hazardous nature

( e.g., ethidium bromide, acetic acid, and boric acid, among others). The researcher is cautioned to exercise care with these reagents and with the equipment used in these procedures, including high voltage power supplies, following the manufacturer's safety recommendations.

1

1

2

Overview

The V15•17 Vertical Gel Electrophoresis Apparatus is designed for easy assembly and reliable performance. Refer to figure 1 to identify the following principal components:

Electrophoresis unit with safety lid, integral gel clamps, and power cords

Glass plates [one short (16.0

!

19.7 cm) and one long plate (18.5

!

with side spacers, bottom spacer, and well-forming comb.

19.7 cm)]

Many of these components and other accessories are available separately. For ordering information, refer to Chapter 5, Related Products.

2

Figure 1. V15•17 Vertical Gel Electrophoresis Apparatus.

Operating Instructions

3.1

Gel Casting

Gels can be cast after assembling the glass plates and spacers, using either the bottom spacer, gel sealing tape, or the V16 Casting Clamp. These techniques are appropriate for casting polyacrylamide gels.

3.1.1 Preparing Gel Plate Assembly Using the Bottom Spacer

1. Make sure the glass plates, spacers, and combs are clean and free of dried gel fragments, grease, and dust. Before the side spacers can be used the first time, a foam block (provided with the spacer set) must be attached 2 mm from the top (unnotched end) of each side spacer.

2. Lay the large glass plate on a clean surface. Place one side spacer along each short edge with the foam blocks at the top. Interlock the bottom spacer with the side spacers (figure 2). When the side and bottom spacers are interlocked properly, no grease is required for good seal.

Short Plate

Long Plate

3

Figure 2. Assembly of Side and Bottom Spacers.

3. Place the short glass plate on top of the spacers so that both sides and the bottom of the plates and the spacers are even. Press the foam blocks at the tops of the side spacers firmly against the top edge of the short glass plate and clamp the assembly together with three spring clips over each side spacer and the bottom spacer (total of nine spring clips). A gap between the foam and the plate will allow buffer to leak out of the upper buffer reservoir during electrophoresis. Therefore, this must be avoided.

Note:

Spring clips should grip the glass plates over the spacers only. Clamping unsupported glass will distort the thickness of the gel.

4. If casting a stacking gel, temporarily insert a well-forming comb and mark the short plate 0.5 to 1 cm below the bottom edge of the teeth to allow space for the stacking gel. Remove the comb before casting the resolving gel.

3

4

Operating

Instructions

Note:

Use only 0.8 or 1.5 -mm thick side spacers with the V16

Casting Clamp.

3.1.2 Preparing Gel Plate Assembly Using the V16 Casting Clamp

1. Make sure the glass plates, spacers, combs and casting clamp are clean and free of dried gel fragments, grease, and dust. Before the side spacers can be used the first time, a foam block (provided with the spacer set) must be attached

2 mm from the bottom (notched end) of each side spacer.

2. Lay the large glass plate on a clean surface. Place one of the side spacers along each short edge with the foam blocks at the top. The V16 Casting Clamp does not require the use of the bottom spacer.

3. Place the small glass plate on top of the spacers so that both sides and the bottom of the glass plates and the spacers are even. Press the foam blocks at the tops of the side spacers firmly against the top edge of the short glass plate.

4. Hold the casting clamp vertically with your thumb resting on the top of the molded handles and your index finger over the molded feet.

5. Flex the clamp outward in a bow-like manner so that the gel sandwich can fit into the bottom of the clamp.

6. Fit the gel sandwich snugly into the bottom of the casting clamp starting at one corner and seal towards the other corner. Once the gel sandwich is seated in the bottom of the clamp, fit the sides of the clamp up onto the sides of the gel sandwich. Start at the bottom and work your way up to the top using your hands to smooth the clamp onto the gel sandwich. Repeat this step for the other side.

7. When properly placed, the sides of the casting clamp will extend to within 4-5 mm of the top of the short glass plate (figure 3). If the sides of the casting clamp should extend further than this, reposition the sides of the casting clamp so that they are at the top of the short glass plate.

8. If casting a stacking gel, temporarily insert a well-forming comb and mark the short plate 0.5 to 1.0 cm below the bottom edge of the teeth to allow space for the stacking gel. Before casting the resolving gel, remove the comb and make sure that the bottom fill port on the casting clamp is sealed.

Glass plates

Port cover

Clamp

Figure 3. Assembly Using the V16 Casting Clamp.

Operating

Instructions

Hint:

Adding 5 µl of a saturated bromophenol blue solution per

10 ml of stacking gel solution gives the stacking gel a pale blue color and makes well visualization and loading easier without affecting gel performance.

Hint:

Clamping the glass over the comb teeth with a spring clip will reduce formation of thin polyacrylamide sheets in the wells.

3.1.3 Pouring Discontinuous Buffer System Gels

1. Prepare the acrylamide solutions without ammonium persulfate (APS). The volumes required are listed in table 1.

3

Warning:

Unpolymerized acrylamide is a known neurotoxin. Please consult the

MSDS before handling this chemical.

Table 1. Gel Solution Volumes as a Function of Gel Thickness.

Gel thickness

(mm)

0.8

1.5

3.0

Stacking gel volume

(ml)

5

10

15

Resolving gel volume

(ml)

25

45

80

2. Add APS to the resolving gel and carefully pour or pipet the gel solution into the gel plate assembly within 10 min, filling to within 1 to 2 mm above the desired level, as marked above. Overlay the solution with 0.5 to 1.5 ml of watersaturated n-butanol to keep the gel surface flat and the interface sharp during polymerization. The assembly should stand vertically and level, while the acrylamide polymerizes.

3. After the resolving gel has polymerized (30 to 45 min), pour off any unpolymerized solution and butanol. Rinse the top of the gel with deionized water.

4. Add APS to stacking gel, then add stacking gel solution to within 2 mm of the top of the short plate.

5. Before inserting the comb, break any small bubbles by touching them with a few

µl of n-butanol. Insert the well-forming comb carefully. Be careful not to trap bubbles under the comb teeth. Allow the gel solution to polymerize completely before removing the comb.

3.1.4 Pouring Continuous Buffer System Gels

1. Fill the gel assembly with acrylamide solution to within 2 to 3 mm of the top of the short plate. Required volumes are listed in table 1. Before inserting the comb, break any small bubbles by touching them with a few µl of n-butanol.

Insert the well-forming comb carefully. Be careful not to trap bubbles under the comb teeth. Allow the gel solution to polymerize, flat, horizontally, completely before removing the comb.

2. The V16 Casting Clamp allows the gel to be filled from the bottom as well as from the top. To fill the gel from the bottom, assemble the gel plates so that when the casting clamp is laying down on the molded feet, the short glass plate is on top. Open the bottom fill port and insert a Luer fitting. Attach a syringe of suitable reservoir to the fitting and slowly fill the gel. Do not interrupt the filling as this may introduce bubbles into the gel solution. Once the gel is filled, insert the well forming comb as before.

3.2

Assembling the Gel Electrophoresis Apparatus

1. Carefully remove the comb. Rinse the comb with deionized water. Rinse off any thin sheets or fragments of polyacrylamide around the wells.

2. Remove the spring clips (and/or tape, if used) from the gel plate assembly.

Carefully remove the bottom spacer, if used. To remove the V16 Casting Clamp, hold the gel assembly with one hand and carefully pull the clamp off starting at the top.

Note:

Acrylamide does not polymerize against Teflon

®

, the material of the side spacers. As a result, a thin channel of unpolymerized acrylamide may remain between the gel and side spacers. Gently press the side spacers inward 0.5 mm to 1 mm to seal this gap.

5

6

Operating

Instructions

Note:

Be sure that the foam blocks are securely down against the top edge of the short glass plate. A gap between the block and the plate will cause buffer to leak out of the upper buffer reservoir during electrophoresis.

3. Place the gel plate assembly into the apparatus with the short plate against the gel sealing gasket. Secure the gel in place with the integral gel clamps. Turn the flippers horizontal to press the gel plates against the gel sealing gasket. Apply pressure evenly to both sides. Do not use excessive force.

4. Place the apparatus on a level surface. Add 400 to 450 ml of electrophoresis buffer to the upper buffer reservoir, and 450 to 500 ml to the lower buffer reservoir. The buffer level in each reservoir should be ~5 mm above the edges of the gel.

Caution:

Before applying power to the apparatus, check for leaks between the gel sealing gasket or foam blocks and the glass plates. Adjust the position of the flippers, the position of the foam blocks, or tension on the integral gel clamps, if necessary. The gasket and blocks will compress and lose resiliency with use, particularly if stored in a compressed state. Replacement parts are available

(see Chapter 5). If no buffer leaks from the top chamber, add buffer to the bottom chamber.

5. Make sure that no bubbles are trapped under the bottom edge of the gel.

Remove bubbles with a syringe and bent needle or curved Pasteur pipette.

3.3

Loading Samples

1. Carefully rinse the wells with electrophoresis buffer to remove bubbles, gel fragments, and any residual unpolymerized acrylamide. If necessary, straighten the well walls with a syringe needle or a fine-tipped micropipette.

2. Load samples into the wells with a syringe or fine-tipped micropipette. Samples should contain 10% (w/v) sucrose, Ficoll

®

, or glycerol to increase sample density and reduce mixing during loading. If the gel is tinted with bromophenol blue, the wells are readily visible. Sample loading volumes are listed in table 2.

Number of teeth

1

(center)

Table 2. Sample Loading Volumes for V15•17 Apparatus Combs as a Function of Gel Thickness.

Tooth width

(mm)

149.7

Gel thickness

(mm)

0.8

1.5

3.0

Nominal capacity/well

(µl)

1,000

2,000

4,000

10 13.0

12

14

20

10.0

8.2

4.8

0.8

1.5

3.0

0.8

1.5

3.0

0.8

1.5

3.0

0.8

1.5

3.0

59

110

220

34

63

126

94

175

350

72

135

270

Note:

All loading volumes are calculated for a sample depth of 9 mm.

Operating

Instructions

3.4

Electrophoresis

1. Place the safety interlock lid over the gel and attach the power cords first to the apparatus, then to the power supply.

2. Turn on the power supply and adjust the settings to the desired voltage or current. Routine settings are 120 to 250 V and 50 mA for a 1.5-mm thick gel.

3

3.5

Post-Electrophoresis

1. At the end of the electrophoresis period, turn off the power supply. Disconnect the leads first from the power supply, then from the apparatus. Remove the safety interlock lid.

2. Carry the apparatus over to a sink and discard the electrophoresis buffer by pouring it out over the front of the apparatus.

3. Unclamp the gel plate assembly from the apparatus. Lay the gel plate assembly on a paper towel. Use a thin spatula to carefully pry the upper glass plate away from the gel.

4. Transfer the gel to a container of stain, fixative, or transfer buffer for further processing.

5. Rinse all apparatus components thoroughly in deionized water and wipe or air dry.

Note:

Do not store glass plates clamped to the unit.

7

Troubleshooting Guide

4

8

Many apparatus problems can be solved by reading and carefully following the instructions in the manual. Some suggestions for troubleshooting are given below. Should these suggestions not resolve the problem, please call the T

ECH

-

L

INE

SM

(numbers are listed on the back cover). If the unit must be returned for repair, contact the Customer Service

Department or your local distributor for shipping instructions. Please include a full description of the problem.

Problem

The glass plates crack.

Comment

The current or voltage setting is too high, causing overheating.

Gel solution leaks from the bottom during casting.

Buffer leaks slowly from the upper reservoir.

Check that the bottom spacer tabs are fully and snugly seated in the side spacer notches.

Apply a small bead of grease to the spacer joints.

Seal the bottom of the gel assembly with a small amount of the resolving gel before casting the full gel.

Add sufficient polymerization catalyst to a 5-ml aliquot of resolving gel solution to cause polymerization in 3 to 5 min, then pour this aliquot into the bottom of the gel assembly and allow it to polymerize.

If using the V16 Casting Clamp, check that the inner surface of the casting clamp is clean and free of debris and grease. Also make sure the bottom of the glass plates are firmly seated against the bottom of the casting clamp.

For continuous buffer system gels, after filling the assembly and inserting the comb, tilt the assembly back on the long plate to a 5° to 10° angle to reduce hydrostatic pressure on the bottom spacer and joints while it polymerizes. Be careful not to overfill the assembly.

Adjust the spring clips slightly to improve the sealing of the gasket against the glass plate.

Verify that the gel sealing gasket is intact and the spacer foam blocks are compressed against the upper edge of the short glass plate.

A spatula may be used to push down on the top of the foam blocks to improve seal.

Press the side spacers inward against the gel to eliminate any channel formed by unpolymerized gel solution.

Check the gel sealing gasket and foam blocks for tears, wrinkles, or signs of compression and loss of resiliency. Replace, if necessary.

After the gel plate assembly has been placed in the apparatus, push down on the top of the side spacer to compress the spacer foam block against the short glass plate.

The gel dye front is not straight.

Bands are distorted or streaked.

Verify that the apparatus is level.

Verify that the buffer depth is sufficient to cover the upper and lower edges of the gel evenly by

"

5 mm.

Remove any bubbles blocking the bottom edge of the gel.

The gel may be overheating. Reduce the voltage or current.

Check that the gel is not being cooled unevenly by a draft or breeze.

Sample may contain excess salt. Dialyze or desalt before loading on gel.

Sample may be too concentrated. Dilute sample or reduce voltage.

Sample may contain precipitated material. Centrifuge or filter before loading on gel.

Dye migration is slower than expected.

The electrophoresis buffer may be too concentrated. Check buffer preparation procedure. If the concentration is high, at constant voltage the current will be higher than usual; at constant current, the voltage will be lower than usual.

Check voltage or current settings on power supply.

Check for secure connections of power cords to the power supply and the apparatus.

Dye migration is faster than expected.

The electrophoresis buffer may be too dilute. Check buffer preparation procedure.

Check voltage or current settings on power supply.

Related Products

Product

Accessories:

Preparative Teflon ® Combs with marker lanes:

Size

Analytical Teflon Combs

10-tooth:

12-tooth:

14-tooth:

0.8-mm thick

1.5-mm thick

3.0-mm thick

0.8-mm thick

1.5-mm thick

3.0-mm thick

0.8-mm thick

1.5-mm thick

3.0-mm thick

0.8-mm thick

1.5-mm thick

3.0-mm thick

Spacer Set

Complete with 1 bottom and 2 side spacers and 2 foam blocks:

Spacer Foam Blocks

Glass Plates

Spring Clips

Stainless Steel Spring Clips

V16 Casting Clamp

Gel Sealing Tape (1.5 in.

!#

72 yd.

$

Replacement Parts:

Replacement Gasket with 6 foam blocks

Upper Platinum Wire Replacement

Lower Platinum Wire Replacement

Gel Clamp Replacement Kit

(Includes: 1 spring, 1 shoulder washer, 1 washer,

1 tab, 1 knob, and 1 support ring)

Power Cord Replacements

(Includes: 1 red and 1 black)

0.8-mm thick

1.5-mm thick

3.0-mm thick pkg. of 12 pkg. of 3 pr.

pkg. of 12 pkg. of 12 pkg. of 1 pkg. of 2

1 roll

10 rolls

48 in.

-----

-----

-----

-----

Cat. No.

11956-117

11956-125

11956-133

11956-026

11956-059

11956-083

11956-034

11956-067

11956-091

11956-042

11956-075

11956-109

41077-017

41077-025

41077-033

21070-057

11074-010

11098-019

21105-333

21070-065

21070-073

11032-018

11032-026

21960-059

11958-345

11958-329

11958-352

11099-041

5

9

10

Related Products

Product

Wire Hardware Replacement Kit

(Includes: 2 O-rings, 1 red cap nut, 1 black cap nut,

2 rubber washers, 2 electrode boots, and 2 hex nuts)

Banana Plug Replacement Kit (see figure 4)

(Includes: 2 banana plugs, 2 O-rings, 1 red cap nut,

1 black cap nut, 2 rubber washers, 2 electrode boots,

2 hex nuts, 2 long ratchet rivits, 2 short ratchet rivits,

2 thin washers, and 2 thick washers)

Cap nut

Platinum wire

Hex nut

Boot

Size

-----

-----

Rubber washer

O-ring

Plug block

Cat. No.

11958-212

11958-378

Unit

Figure 4. Cross Section of Banana Plug Assembly

Apparatus:

Model 250 Power Supply

Model 250EX Power Supply

Reagents:

Acrylamide

Ammonium Persulfate

BluPrint Fast-PAGE Stain

C

OOMASSIE

B

RILLANT

Protein Stain

B

LUE

R ® 250

Glycine

N,N´–Methylenebisacrylamide

TEMED

Tris

Sodium Dodecyl Sulfate

Molecular Size Standards:

B

ENCH

M

ARK

™ Protein Ladder

B

ENCH

M

ARK

Prestain Protein Ladder

10 kDa Protein Ladder

Prestained Protein Molecular Weight

Standards, High Range

-----

-----

100 g

100 g

100 ml

10 g

500 g

25 g

30 ml

500 g

100 g

2 x 250 µl

2 x 250 µl

1 ml

500 µl

11066-016

11066-081

15512-015

15523-012

15587-017

15528-011

15527-013

15516-016

15524-010

15504-012

15525-025

10747-012

10748-010

10064-012

26041-020

Additional Information

6.1 Care and Handling

The Model V15•17 Apparatus is fabricated from high quality acrylic plastic possessing very good heat, impact, and chemical resistance. As with any laboratory instrument, adequate care will assure consistently reliable service.

Caution:

Platinum electrodes are very fragile and not warranted against breakage.

To avoid damage, use extreme care when cleaning this apparatus.

After each use, rinse well with deionized water. Wipe dry with a soft cloth or paper towel, or allow to air dry. Whenever necessary, all components may be washed gently with water and a nonabrasive detergent, and rinsed and dried as above. To remove grease and oils, use a light application of hexane, kerosene, or aliphatic naphtha.

Never use abrasive cleaners, window sprays, or scouring pads to clean the components, as these can cause surface damage.

Prolonged clamping pressure will compress the sealing gasket. Storing the apparatus unassembled between runs allows the gasket to decompress, regaining its original resiliency. Constant, heavy duty service may eventually result in permanent gasket compression and possible buffer leakage from the upper chamber. A replacement gasket is provided with each system. If replacement is necessary, remove the old gasket and adhesive. Clean the surface thoroughly with a mild detergent and apply the new gasket according to instructions provided in the package.

Additional cautions:

Do not autoclave or dry-heat sterilize the apparatus or components.

Do not expose the apparatus or components to phenol, acetone, benzene, halogenated hydrocarbon solvents, or undiluted laboratory alcohols.

Avoid prolonged exposure of the apparatus or components to UV light.

6.2 Maintenance

The following simple, routine inspection and maintenance procedures will help ensure both the safety and the performance of the Model V15•17 Vertical Gel

Electrophoresis Apparatus. For ordering information on replacement parts, refer to

Chapter 5 or call the Life Technologies T

ECH

-L

INE

SM .

Inspect electrical connections and power cords regularly. If the banana plug connectors to either the apparatus or the power supply do not exhibit reasonable friction or can be rocked easily when connected, replace the banana plugs or power cords as appropriate.

If power cords show any signs of wear or damage ( e.g., cracks, nicks, abrasions, melted insulation, or bare wire), replace the cords immediately.

Examine the electrode banana plugs and electrode connection nuts on the apparatus to ensure that they are free of corrosion. If the plugs or nuts are corroded, they should be replaced.

Examine the gel sealing gasket and spacer foam blocks for tears, wrinkles, or compression. Replace any damaged or permanently compressed foam parts.

6

11

12

Additional

Information

6.3 Specifications

“Installation Category I”

Weight................................................................................................................................. 1.75 kg (3.85 lb.)

Dimensions (W × D × H) ..............................................................................................29.2

!

17.5

!

22.4 cm

(11.5

!

6.9

!

8.8 in.)

Gel Dimensions (W × H, using bottom spacer)...............................................17.1

!

14.7 cm (6.75

!

5.8 in.)

Voltage Range..........................................................................................................................250 VDC Max

Current Range...................................................................................................................................0-50 mA

Operating Temperature Range...........................................................4-30°C (non-condensing atmosphere)

Construction ................................................................................................................................acrylic, ABS

Electrode material .............................................................................................................................platinum

Maximum gel thickness .........................................................................................................................3 mm

Working buffer volume ..................................................................................................................... 1,000 ml

Comb and spacer set included ...................................................................................20-tooth, 1.5-mm thick

Comb material ......................................................................................................................................Teflon

6.4 Warranty

Life Technologies warrants, to the original purchaser, apparatus of its manufacture against defects in materials and workmanship, under normal service, for one year from the date of receipt by the purchaser. This warranty excludes damages resulting from shipping, misuse, carelessness, or neglect. Life Technologies’ liability under the warranty is limited to the repair of such defects or the replacement of the product, at our option, and is subject to receipt of reasonable proof by the customer that the defect is embraced within the terms of the warranty. All claims made under this warranty must be presented to Life Technologies within one year following the date of delivery of the product to the customer.

This warranty is in lieu of any other warranties or guarantees, expressed or implied, arising by law or otherwise. Life Technologies makes no other warranty, expressed or implied, including warranties or merchantability or fitness for a particular purpose. Under no circumstances shall Life

Technologies be liable for damages either consequential, compensatory, incidental, or special, sounding in negligence, strict liability, breach of warranty, or any other Theory, arising out of the use of the product listed herein.

Life Technologies reserves the right to make improvements in design, construction, and appearance of this product without notice.

6.5 Declaration of Conformity and CE Mark

Note:

The information outlined in this section applies only to customers located in the European Union (EU). The EU is currently comprised of 15 member countries.

This laboratory apparatus is identified with the

CE

mark. This mark indicates that the product complies to the following EU Directives and Standards:

Application of Council Directive(s):

73/23/EEC Low Voltage Directive

Standards:

EN 61010-1:1993 Product Safety

EU Representative:

Life Technologies Ltd.

EU Address:

3 Fountain Dr.

Inchinnan Business Park

Paisley, PA49RF Scotland

A copy of the Declaration of Conformity certificate is available upon request.

Part No.

Lot No.

14276

1085863

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