Accela 600 Pump and 1250 Pump Hardware Manual Rev.F

Accela 600 Pump and 1250 Pump Hardware Manual Rev.F
Accela 600 Pump
and 1250 Pump
Hardware Manual
60157-97010 Revision F
March 2011
© 2011 Thermo Fisher Scientific Inc. All rights reserved.
Thermo Foundation, Exactive, MSQ, and TSQ are trademarks and Accela, ChromQuest, Xcalibur, and LTQ
are registered trademarks of Thermo Fisher Scientific in the United States.
The following are registered trademarks in the United States and other countries:
Valco is a registered trademark of Valco Instruments Co. Inc. and VICI AG. Fluorinert is a registered
trademark of 3M. Fomblin is a registered trademark of Solvay Solexis S.p.A. Teflon, Tefzel, and Freon are
registered trademarks of E.I. du Pont de Nemours and Company. Hastelloy is a registered trademark of Haynes
International, Inc. Luer-Lok is a registered trademark of Becton, Dickinson and Co.
PEEK is a trademark of Victrex plc.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or errorfree and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history: Revision A, August 2009; Revision B, October 2009; Revision C, March 2010; Revision D,
April 2010; Revision E, February 2011; Revision F, March 2011
Revision F contains typographical error corrections.
Software versions: ChromQuest 4.2 or later, Xcalibur 2.0.7 (LC Devices 2.2.0 SP1), Xcalibur 2.1.x or later
(LC Devices 2.2.1 to 2.5.0), Thermo Foundation 1.0.x (for the Xcalibur 2.1.x data system)
For Research Use Only. Not for use in diagnostic procedures.
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent
electromagnetic compatibility (EMC) and safety standards as described in the next section or sections by product name.
Changes that you make to your system may void compliance with one or more of these EMC and safety standards.
Changes to your system include replacing a part or adding components, options, or peripherals not specifically
authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards,
replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific
or one of its authorized representatives.
Accela 600 Pump
EMC Directive 2004/108/EC
EMC compliance has been evaluated by TUV Rheinland of North America Inc.
EN 55011: 2007
EN 61000-4-4: 2004
EN 61000-3-2: 2006
EN 61000-4-5: 2005
EN 61000-3-3: 1995, A1: 2001, A2: 2005
EN 61000-4-6: 2007
EN 61000-4-2: 1995, A1: 1999, A2: 2001
EN 61000-4-11: 2004
EN 61000-4-3: 2006
EN 61326-1: 2006
FCC Class A: CFR 47, Part 15: 2007
Low Voltage Safety Compliance
This device complies with Low Voltage Directive 2006/95/EC and the following harmonized standards:
EN 61010-1: 2001, IEC 61010-1: 2002, UL 61010A-1: 2004, CAN/CSA 22.2 61010-1: 2004.
Accela 1250 Pump
EMC Directive 2004/108/EC
EMC compliance has been evaluated by TUV Rheinland of North America Inc.
EN 55011: 2007
EN 61000-4-3: 2006
EN 61000-3-2: 2006
EN 61000-4-4: 2004
EN 61000-3-3: 1995, A1: 2001, A2: 2005
EN 61000-4-5: 2005
EN 61000-4-2: 1995, A1: 1999, A2: 2001
EN 61000-4-6: 2007
EN 61326-1: 2006
EN 61000-4-11: 2004
FCC Class A: CFR 47, Part 15: 2009
Low Voltage Safety Compliance
This device complies with Low Voltage Directive 2006/95/EC and the following harmonized standards:
EN 61010-1: 2001, IEC 61010-1: 2002, UL 61010A-1: 2004, CAN/CSA 22.2 61010-1: 2004.
FCC Compliance Statement for the Accela 600 and 1250 Pumps
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside
this manual pertaining to the safe use and operation of this product before using the device.
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or
bulky for one person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency
transmitters, such as mobile phones, in close proximity to the instrument.
For manufacturing location, see the label on the instrument.
WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union
(EU) Member State, and these companies should dispose of or recycle this product. See www.thermo.com/
WEEERoHS for further information on Thermo Fisher Scientific’s compliance with these Directives and the
recyclers in your country.
WEEE Konformität
Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC erfüllen.
Das Produkt ist durch folgendes Symbol gekennzeichnet:
Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten
getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information
über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise,
die nützlich sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter
www.thermo.com/WEEERoHS.
Conformité DEEE
Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et
Electroniques (DEEE). Il est marqué par le symbole suivant:
Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de
l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage d'informations sur la
conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre pays et les informations sur les
produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont
disponibles sur www.thermo.com/WEEERoHS.
CAUTION Symbol
CAUTION
VORSICHT
ATTENTION
PRECAUCION
AVVERTENZA
Electric Shock: This instrument uses
high voltages that can cause personal
injury. Before servicing, shut down the
instrument and disconnect the instrument
from line power. Keep the top cover on
while operating the instrument. Do not
remove protective covers from PCBs.
Elektroschock: In diesem Gerät werden
Hochspannungen verwendet, die
Verletzungen verursachen können. Vor
Wartungsarbeiten muß das Gerät
abgeschaltet und vom Netz getrennt
werden. Betreiben Sie Wartungsarbeiten
nicht mit abgenommenem Deckel. Nehmen
Sie die Schutzabdeckung von Leiterplatten
nicht ab.
Choc électrique: L’instrument utilise des
tensions capables d’infliger des blessures
corporelles. L’instrument doit être arrêté et
débranché de la source de courant avant
tout intervention. Ne pas utiliser
l’instrument sans son couvercle. Ne pas
enlever les étuis protecteurs des cartes de
circuits imprimés.
Descarga eléctrica: Este instrumento
utiliza altas tensiones, capaces de
producir lesiones personales. Antes de
dar servicio de mantenimiento al
instrumento, éste debera apagarse y
desconectarse de la línea de alimentacion
eléctrica. No opere el instrumento sin sus
cubiertas exteriores quitadas. No remueva
las cubiertas protectoras de las tarjetas
de circuito impreso.
Shock da folgorazione. L’apparecchio è
alimentato da corrente ad alta tensione
che puo provocare lesioni fisiche. Prima di
effettuare qualsiasi intervento di
manutenzione occorre spegnere ed isolare
l’apparecchio dalla linea elettrica. Non
attivare lo strumento senza lo schermo
superiore. Non togliere i coperchi a
protezione dalle schede di circuito
stampato (PCB).
Chemical: This instrument might contain
hazardous chemicals. Wear gloves when
handling toxic, carcinogenic, mutagenic,
or corrosive or irritant chemicals. Use
approved containers and proper
procedures to dispose waste oil.
Chemikalien: Dieses Gerät kann
gefährliche Chemikalien enthalten. Tragen
Sie Schutzhandschuhe beim Umgang mit
toxischen, karzinogenen, mutagenen oder
ätzenden/reizenden Chemikalien.
Entsorgen Sie verbrauchtes Öl
entsprechend den Vorschriften in den
vorgeschriebenen Behältern.
Chimique: Des produits chimiques
dangereux peuvent se trouver dans
l’instrument. Portez des gants pour
manipuler tous produits chimiques
toxiques, cancérigènes, mutagènes, ou
corrosifs/irritants. Utiliser des récipients
et des procédures homologuées pour se
débarrasser des déchets d’huile.
Química: El instrumento puede contener
productos quimicos peligrosos. Utilice
guantes al manejar productos quimicos
tóxicos, carcinogenos, mutagenos o
corrosivos/irritantes. Utilice recipientes y
procedimientos aprobados para
deshacerse del aceite usado.
Prodotti chimici. Possibile presenza di
sostanze chimiche pericolose
nell’apparecchio. Indossare dei guanti per
maneggiare prodotti chimici tossici,
cancerogeni, mutageni, o
corrosivi/irritanti. Utilizzare contenitori
aprovo e seguire la procedura indicata per
lo smaltimento dei residui di olio.
Heat: Before servicing the instrument,
allow any heated components to cool.
Hitze: Warten Sie erhitzte Komponenten
erst nachdem diese sich abgekühlt haben.
Haute Temperature: Permettre aux
composants chauffés de refroidir avant
tout intervention.
Altas temperaturas: Permita que lop
componentes se enfríen, ante de efectuar
servicio de mantenimiento.
Calore. Attendere che i componenti
riscaldati si raffreddino prima di
effetturare l’intervento di manutenzione.
Fire: Use care when operating the system
in the presence of flammable gases.
Feuer: Beachten Sie die einschlägigen
VorsichtsmaBnahmen, wenn Sie das
System in Gegenwart von entzündbaren
Gasen betreiben.
Incendie: Agir avec précaution lors de
l’utilisation du système en présence de
gaz inflammables.
Fuego: Tenga cuidado al operar el
sistema en presencia de gases
inflamables.
Incendio. Adottare le dovute precauzioni
quando si usa il sistema in presenza di gas
infiammabili.
Eye Hazard: Eye damage could occur
from splattered chemicals or flying
particles. Wear safety glasses when
handling chemicals or servicing the
instrument.
Verletzungsgefahr der Augen:
Verspritzte Chemikalien oder kleine
Partikel können Augenverletzungen
verursachen. Tragen Sie beim Umgang mit
Chemikalien oder bei der Wartung des
Gerätes eine Schutzbrille.
Danger pour les yeux: Des projections
chimiques, liquides, ou solides peuvent
être dangereuses pour les yeux. Porter des
lunettes de protection lors de toute
manipulation de produit chimique ou pour
toute intervention sur l’instrument.
Peligro par los ojos: Las salicaduras de
productos químicos o particulas que
salten bruscamente pueden causar
lesiones en los ojos. Utilice anteojos
protectores al mnipular productos
químicos o al darle servicio de
mantenimiento al instrumento.
Pericolo per la vista. Gli schizzi di
prodotti chimici o delle particelle presenti
nell’aria potrebbero causare danni alla
vista. Indossare occhiali protettivi quando
si maneggiano prodotti chimici o si
effettuano interventi di manutenzione
sull’apparecchio.
General Hazard: A hazard is present that
is not included in the above categories.
Also, this symbol appears on the
instrument to refer the user to instructions
in this manual.
Allgemeine Gefahr: Es besteht eine
weitere Gefahr, die nicht in den
vorstehenden Kategorien beschrieben ist.
Dieses Symbol wird im Handbuch
auBerdem dazu verwendet, um den
Benutzer auf Anweisungen hinzuweisen.
Danger général: Indique la présence
d’un risque n’appartenant pas aux
catégories citées plus haut. Ce symbole
figure également sur l’instrument pour
renvoyer l’utilisateur aux instructions du
présent manuel.
Peligro general: Significa que existe un
peligro no incluido en las categorias
anteriores. Este simbolo también se utiliza
en el instrumento par referir al usuario a
las instrucciones contenidas en este
manual.
Pericolo generico. Pericolo non
compreso tra le precedenti categorie.
Questo simbolo è utilizzato inoltre
sull’apparecchio per segnalare all’utente
di consultare le istruzioni descritte nel
presente manuale.
When the safety of a procedure is
questionable, contact your local Technical
Support organization for Thermo Fisher
Scientific San Jose Products.
Wenn Sie sich über die Sicherheit eines
Verfahrens im unklaren sind, setzen Sie
sich, bevor Sie fortfahren, mit Ihrer
lokalen technischen
Unterstützungsorganisation für Thermo
Fisher Scientific San Jose Produkte in
Verbindung.
Si la sûreté d’une procédure est
incertaine, avant de continuer, contacter
le plus proche Service Clientèle pour les
produits de Thermo Fisher Scientific San
Jose.
Cuando la certidumbre acerca de un
procedimiento sea dudosa, antes de
proseguir, pongase en contacto con la
Oficina de Asistencia Tecnica local para
los productos de Thermo Fisher Scientific
San Jose.
Quando e in dubbio la misura di sicurezza
per una procedura, prima di continuare, si
prega di mettersi in contatto con il
Servizio di Assistenza Tecnica locale per i
prodotti di Thermo Fisher Scientific San
Jose.
CAUTION Symbol
CAUTION
Electric Shock: This instrument uses
high voltages that can cause personal
injury. Before servicing, shut down the
instrument and disconnect the instrument
from line power. Keep the top cover on
while operating the instrument. Do not
remove protective covers from PCBs.
Chemical: This instrument might contain
hazardous chemicals. Wear gloves when
handling toxic, carcinogenic, mutagenic,
or corrosive or irritant chemicals. Use
approved containers and proper
procedures to dispose waste oil.
Heat: Before servicing the instrument,
allow any heated components to cool.
Fire: Use care when operating the system
in the presence of flammable gases.
Eye Hazard: Eye damage could occur
from splattered chemicals or flying
particles. Wear safety glasses when
handling chemicals or servicing the
instrument.
General Hazard: A hazard is present that
is not included in the above categories.
Also, this symbol appears on the
instrument to refer the user to instructions
in this manual.
When the safety of a procedure is
questionable, contact your local Technical
Support organization for Thermo Fisher
Scientific San Jose Products.
C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xv
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Good Laboratory Practices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xviii
Keeping Good Records . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xviii
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Thermo Scientific
Chapter 1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Hardware Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Vacuum Degasser Assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Liquid Displacement Assembly (LDA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Purge Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Dynamic Mixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Seal Wash Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Leak Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Status LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 2
Accela 600 or 1250 Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Accela Pump Installation Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Unpacking and Inspecting the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Making the Initial Instrument Preparations. . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Layout for an Accela LC System with an Accela Autosampler . . . . . . . . . . . . 11
Layout for an Accela LC System with an Accela Open Autosampler . . . . . . . 13
Back Panel Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Connecting the Power Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Connecting the Pump to the Data System Computer . . . . . . . . . . . . . . . . . . 17
Connecting the Accela System Interconnect Cables. . . . . . . . . . . . . . . . . . . . 17
Additional Hardware Configurations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Connecting the Low-Pressure Solvent Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Assembling the Solvent Reservoir Bottles. . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Connecting the Solvent Reservoir Bottles to the Degassing Unit . . . . . . . . . . 25
Priming the Degassing Membranes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Accela 600 and 1250 Pump Hardware Manual
xi
Contents
Connecting the Solvent Line Between the Pump and the Autosampler . . . . . . . 27
Connecting the High-Pressure Solvent Line to the Pump Outlet. . . . . . . . . . 28
Connecting the High-Pressure Solvent Line to the Autosampler . . . . . . . . . . 29
Powering On the Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
xii
Chapter 3
Hardware Upgrades . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Dynamic Mixer Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Removing the Pump Chassis from the Enclosure. . . . . . . . . . . . . . . . . . . . . . 35
Connecting the Dynamic Mixer to the Pump’s Front Panel . . . . . . . . . . . . . 37
Connecting the Dynamic Mixer to the Controller Board . . . . . . . . . . . . . . . 41
Reconnecting the Pump Chassis to the Enclosure . . . . . . . . . . . . . . . . . . . . . 42
Reconnecting the Static Mixer and the High-Pressure Lines . . . . . . . . . . . . . 42
Seal Wash Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Leak Sensor Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Setting Up Continuous Flushing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Chapter 4
Instrument Driver Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59
ChromQuest Instrument Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Xcalibur Instrument Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Thermo Foundation Instrument Configuration . . . . . . . . . . . . . . . . . . . . . . . . 66
Adding Pump 1 to the Instrument Configuration . . . . . . . . . . . . . . . . . . . . . 66
Adding Pump 2 to the Instrument Configuration . . . . . . . . . . . . . . . . . . . . . 69
Specifying the Leak Sensor Settings in the Xcalibur Data System . . . . . . . . . . . 70
Chapter 5
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73
Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Accessing the LDA Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Removing the LDA from the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Dismantling the LDA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Reassembling the LDA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Reattaching the LDA to the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Replacing the Primary Piston Seals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Replacing the Secondary Piston Seals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
Cleaning the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Cleaning the Pistons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Cleaning the Instrument Casing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Flushing the LDA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Flushing the Piston Guide Bushings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Removing Air from the Piston Chambers . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Cleaning the Displacement Chambers and the Inlet Module. . . . . . . . . . . . . . 102
Replacing the Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
Contents
Chapter 6
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
Diagnostic Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Testing the Flow Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Testing the Residual Pulsation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Testing the Pump Proportioning Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . 108
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Troubleshooting Guides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Pump Troubleshooting Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Degasser Troubleshooting Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Chapter 7
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
Main Body . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Accela 600 Pump LDA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Accela 1250 Pump LDA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Inlet Valve Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Service Spares . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Accela 600 Pump Accessory Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Accela 1250 Pump Accessory Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Inlet Tubing Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
Compression Fittings for High-Pressure Connections . . . . . . . . . . . . . . . . . . . 127
Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Seal Wash Pump Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
Dynamic Mixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
Leak Sensor Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
xiii
P
Preface
This manual describes how to install, maintain, and troubleshoot the Thermo Scientific
Accela™ 600 Pump and Accela 1250 Pump. You can operate the Accela pumps from a
computer with the ChromQuest™ data system or the Xcalibur™ data system (version 2.0.7
or later).
Contents
• Related Documentation
• Safety Information
• Environmental Conditions
• Good Laboratory Practices
• Keeping Good Records
• Contacting Us
Related Documentation
In addition to this manual, you can find information related to the Accela 600 Pump and the
Accela 1250 Pump in the following documents provided as PDF files and Help accessed from
the data system:
• Accela user guide for your Thermo Scientific data system —Provides instructions on how
to control the Accela LC system from your Thermo Scientific data system.
• Accela Preinstallation Requirements Guide—Provides information about the appropriate
laboratory setup for your Accela LC system.
• Accela Getting Connected Guide—Provides instructions on how to set up your Accela LC
system and interconnecting the system modules.
• Accela Pump Help for the ChromQuest data system—Provides instructions on how to
control the Accela pumps from the ChromQuest data system.
• Thermo Pump Help for LC Devices—Provides instructions on how to configure the
pumps from the Thermo Foundation application and control the pumps from the
Xcalibur data system.
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
xv
Preface
Safety and Special Notices
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.
CAUTION Highlights electric shock hazards to humans. Each electric shock notice is
accompanied by the international high voltage symbol.
CAUTION Highlights chemical hazards to humans, property, or the environment. Each
chemical notice is accompanied by the chemical caution symbol.
Note Highlights information of general interest.
Safety Information
Observe the precautions listed in this section to ensure the safe operation and longevity of the
instrument.
CAUTION When you use the pump, follow the generally accepted procedures for quality
control and method development. If you use the pump in the field of chromatographic
analysis and you observe a change in retention time of a particular compound, in the
resolution between two compounds, or in peak shape, immediately determine the reason
for the changes. Until you determine the cause of the change, do not rely on separation
results.
CAUTION Use equipment only in the manner specified by its manufacturer, or risk
impairing the protection provided by the equipment.
CAUTION Do not service any part when operating the instrument. Do not remove the
front panel when the instrument is running. Do not run the instrument without the front
panel.
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Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
Preface
Safety and Special Notices
CAUTION The instrument contains voltage lines. Switch off the power and disconnect
the power cable prior to servicing the instrument. There is no need to open the pump
enclosure, as all user serviceable components are outside of the instrument.
CAUTION Follow the maintenance procedures in this manual when replacing or repairing
the pump’s serviceable components. Never try to repair or replace components not
described in this manual without the assistance of a Thermo Fisher Scientific field service
representative.
CAUTION To prevent personal injury, observe good laboratory practice when handling
solvents, changing tube lines. or both. Consult the pertinent material safety data sheets
(MSDSs) for the solvents used for HPLC analysis.
Environmental Conditions
The installation category (over voltage category) for this instrument is Category 2, which
pertains to equipment that receives its electrical power from the local level, such as an
electrical wall outlet.
The pollution degree for this instrument is Pollution Category 2 (Laboratory): Normally, only
nonconductive pollution occurs. Expect temporary conductivity caused by condensation.
Observe the following environmental specifications.
CAUTION Only use the pump according to the conditions stated, or risk personal injury
or damage to the pump.
Table 1. Environmental specifications
Thermo Scientific
Environmental condition
Recommendation
Location
Indoor use only
Altitude
Up to 2000 meters
Temperature
5 to 40 °C (41 °F to 104 °F)
Maximum relative humidity
80% for temperatures up to 31 °C (88 °F)
non-condensing, decreasing linearly to 50% relative
humidity at 40 °C (104 °F)
Voltage fluctuation limit
Not to exceed ± 10% of the nominal voltage
Accela 600 and 1250 Pump Hardware Manual
xvii
Preface
Safety and Special Notices
Good Laboratory Practices
To obtain optimal performance from your ultra-high-pressure liquid chromatography
(UHPLC) system and to prevent personal injury or injury to the environment, do the
following:
• Keep good records.
• Read the manufacturers’ MSDSs for the chemicals you use in your laboratory.
• Remove particulate matter from your samples before injecting them into the liquid
chromatograph.
• Use HPLC-grade or LC/MS-grade solvents
• Connect the drainage tubes from the pump, autosampler, and detector to an appropriate
waste receptacle. Dispose of solvents as specified by local regulations.
Keeping Good Records
To help identify and isolate problems with either your equipment or your methodology, keep
good records of all system conditions (for example, %RSDs on retention times and peak areas,
peak shape, and resolution). At a minimum, keep a chromatogram of a typical sample and
standard mixture, well documented with system conditions, for future reference. Careful
comparison of retention times, peak shapes, peak sensitivity, and baseline noise can provide
valuable clues to identifying and solving future problems.
Chemical Toxicity
Although the large volume of toxic and flammable solvents used and stored in laboratories can
be quite dangerous, do not ignore the potential hazards posed by your samples. Take special
care to read and follow all precautions that ensure proper ventilation, storage, handling, and
disposal of both solvents and samples. Become familiar with the toxicity data and potential
hazards associated with all chemicals by referring to the manufacturers’ MSDSs.
Solvent Requirements
Use HPLC-grade solvents that are free of particulates. Choose a mobile phase that is
compatible with the sample and column you have selected for your separation. Be aware that
some solvents are corrosive to stainless steel.
CAUTION Do not use solvents containing Freon™ and perfluorinated solvents, such as
Fluorinert™ and Fomblin™ perfluoro polyether solvents. They adversely affect the Teflon™
AF degassing membrane.
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Accela 600 and 1250 Pump Hardware Manual
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Preface
Safety and Special Notices
Solvent Disposal
Make sure you have a solvent waste container or other kind of drain system available at or
below the benchtop level. Most solvents have special disposal requirements prohibiting
disposal directly down a drain. Follow all governmental regulations when disposing of any
chemical.
High-Pressure Systems and Leaks
LC systems operate at high pressures. There is little immediate danger from the high pressures
in an LC system. However, if a leak occurs, correct it as soon as possible. Always wear eye and
skin protection when operating or maintaining an LC system. Always shut down the system
and return it to atmospheric pressure before attempting any maintenance.
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
xix
Preface
Safety and Special Notices
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
 To contact Technical Support
Phone
800-532-4752
Fax
561-688-8736
E-mail
[email protected]
Knowledge base
www.thermokb.com
Find software updates and utilities to download at mssupport.thermo.com.
 To contact Customer Service for ordering information
Phone
800-532-4752
Fax
561-688-8731
E-mail
[email protected]
Web site
www.thermo.com/ms
 To get local contact information for sales or service
Go to www.thermoscientific.com/wps/portal/ts/contactus.
 To copy manuals from the Internet
Go to mssupport.thermo.com, agree to the Terms and Conditions, and then click
Customer Manuals in the left margin of the window.
 To suggest changes to documentation or to Help
• Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62.
• Send an e-mail message to the Technical Publications Editor at
[email protected]
xx
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
1
Introduction
This chapter introduces the Accela 600 Pump (see Figure 1) and the Accela 1250 Pump.
Contents
• Functional Description
• Hardware Components
• Status LEDs
• Specifications
Figure 1.
Accela 600 Pump
Power
Thermo Scientific
Communication
Run
Degas
Accela 600 and 1250 Pump Hardware Manual
1
1
Introduction
Functional Description
Functional Description
The 600 and 1250 pumps are quaternary, low-pressure mixing pumps with a built-in solvent
degassing system and an automatic calibration feature. Table 2 lists the flow rate ranges where
these pumps provide optimal performance as well as their maximum operating pressures.
With the latest firmware versions (see Table 5 and Table 6), the minimum programmable flow
rate for both pumps is 1 μL/min.
Tip For gradient applications, Thermo Fisher Scientific recommends that you use a flow
rate equal to or greater than twice the gradient delay volume of the pump’s liquid
displacement assembly (LDA).
Table 2. Flow rate range and maximum operating pressure
Pump
Flow rate range
for optimal performance
Maximum
operating pressure
Accela 600 Pump
50 to 5000 μL/min (isocratic)
180a to 5000 μL/min (gradient)
600 bar (8702 psi)
Accela 1250 Pump
50 to 2000 μL/min (isocratic)
140b to 2000 μL/min (gradient)
1250 bar (18 130 psi)
a
The gradient delay volume of the LDA is 90 μL.
b
The gradient delay volume of the LDA is 70 μL.
These pumps use a force sensor feedback controller, which continuously calibrates valve
timing and pumping efficiency based on the measured compressibility of the solvent. This
patent-pending feature enables these pumps to form accurate gradients virtually
pulsation-free; no pulse damping device is required.
The integral vacuum degasser offers superb solvent degassing efficiency using a built-in
vacuum membrane degasser that removes dissolved gasses from the mobile phase.
The Accela 600 Pump and 1250 Pump are benchtop units that you can integrate with other
Accela instruments.
2
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
1 Introduction
Hardware Components
Hardware Components
The 600 and 1250 pumps have these major components: the vacuum degasser assembly, the
liquid displacement assembly (LDA), a 65 μL static mixer, an optional dynamic mixer, an
optional seal wash pump, and an optional leak sensor. Status LEDs on the left door of the
pump enclosure display the power, communication, run, and degas states of the pump.
Figure 2 shows the front panel of the 600 Pump with the optional seal wash pump and
optional dynamic mixer installed. Figure 46 on page 53 shows the optional leak sensor
installed on the pump drip tray.
Figure 2.
Front panel of the 600 Pump
Liquid displacement
assembly
Vacuum degasser
assembly
Static
mixer
(Optional) dynamic mixer or
outlet adapter
Purge valve
(Optional) seal wash pump or
cover plate
These topics describe the main components of the LC pump:
• Vacuum Degasser Assembly
• Liquid Displacement Assembly (LDA)
• Purge Valve
• Dynamic Mixer
• Seal Wash Pump
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
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1
Introduction
Hardware Components
Vacuum Degasser Assembly
The 600 and 1250 pumps contains a built-in vacuum membrane degasser that removes
dissolved gasses from the mobile phase. Dissolved gasses can cause many problems in HPLC
and must be kept to an absolute minimum for best performance. The solvent degassing
system consists of four independent channels that are maintained at a constant vacuum of
approximately 50 mm Hg absolute. Each channel has a volume of less than 500 μL, which
provides superior degassing efficiency capable of limiting dissolved oxygen to a level of only
0.8 ppm. By varying its speed, the vacuum pump maintains a constant vacuum level. This
eliminates the excess baseline noise and drift caused by vacuum pump cycling.
Liquid Displacement Assembly (LDA)
The frame of the liquid displacement assembly (LDA) consists of two components: the LDA
body and the inlet module. The LDA body contains the inlet and outlet check valves, two
pistons and their seals, upper and lower piston chambers, and various PEEK™ seal rings. The
inlet module contains four high-precision proportioning valves.
The 600 pump has ceramic pistons. Its primary piston seals are made of graphite
fiber-reinforced polytetrafluoroethylene (GFP) and its secondary piston seals are made of
polyethylene (PE).
The 1250 pump has sapphire pistons. Its primary and secondary piston seals are identical and
are made of GFP.
Purge Valve
The purge valve is located to the left of the LDA (see Figure 2 on page 3). Turning the
wingnut counterclockwise opens the purge valve.
When the purge valve is open and the solvent flow is experiencing backpressure from either an
LC column or a backpressure regulator, mobile phase exits the LDA from the left side of the
outlet module.
When the purge valve is closed, mobile phase flows out the front of the LDA assembly and
into the high-pressure tubing that connects the LDA to the mixer or outlet adapter and the
mixer to the autosampler.
Dynamic Mixer
The 600 and 1250 pumps ship with an outlet adapter that you can replace with the optional
dynamic mixer. Adding the optional dynamic mixer to the Accela or Transcend pump ensures
the mixing of complex or relatively incompatible solvents and reduces the chromatographic
baseline noise for applications using proportioned mobile phases. The integrated dynamic
4
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
1 Introduction
Hardware Components
mixer also increases the accuracy and reproducibility of complex ternary and quaternary
gradients. The dynamic mixer adds 35 μL of gradient delay volume to the system. You can
plumb the system to bypass the dynamic mixer for mobile phases that do not require
additional mixing.
Seal Wash Pump
You can also add an optional seal wash pump to the 600 and 1250 pumps for continuous
flushing of the piston guide bushings. Use the seal wash pump for optimal pump performance
when working with highly concentrated buffer solutions.
As the pistons move back and forth within the displacement chambers, a small quantity of
mobile phase leaks behind the primary piston seals. With buffered mobile phases, a precipitate
(also known as creep or salt build-up) forms as the liquid fraction evaporates. The abrasion
caused by this precipitate can scratch the piston and shorten the life of the piston seals. With
the seal wash pump option, a large reservoir above the pump continuously feeds the pump
and flushes the piston guide bushings to prevent the precipitation of salt crystals behind the
primary piston seals.
Leak Sensor
In addition to the dynamic mixer and the seal wash pump, you can add an optional leak
sensor that detects the presence of liquid on the pump’s drip tray.
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
5
1
Introduction
Status LEDs
Status LEDs
The 600 and 1250 pumps have four status LEDs on the left door that provide information
about the pump. Table 3 describes the states of the four status LEDs.
Table 3. Status LEDs for the pump
LED
Status
Description
Power
Green
The pump is switched on and is receiving power.
Comm
Amber
The pump is not communicating with the data system
computer.
Green
Communication to the data system computer has been
established.
Flashing green
A pump program is downloading from the data system
computer.
Amber
The power is switched on, but the pump pistons are idle,
producing no flow.
Flashing amber
A firmware download is in progress or an error condition
has occurred.
Green
The pump pistons are moving, but a pump program is not
running. This can occur when the pump is under direct
control or a pump program has ended.
Flashing green
The pump is running a pump program from a downloaded
method.
Amber
The degas unit is building vacuum.
Flashing amber
A failure, such as a loss of vacuum, has occurred.
Green
Sufficient vacuum has developed to perform liquid
chromatography.
Run
Degas
6
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
1
Introduction
Specifications
Specifications
Table 4 through Table 8 list the pump’s physical dimensions, pump and degasser operating
specifications, and electronic specifications.
Thermo Fisher Scientific provides periodic firmware updates that are backward compatible
with all hardware versions of the pump. For information about firmware updates, contact
your local Thermo Fisher Scientific field service representative.
Table 4. Physical dimensions
Specification
Description
Width
36.0 cm (14 in.)
Depth
45.7 cm (18 in.)
Height
18.0 cm (7.1 in.)
Weight
19.5 kg (43 lbs)
Table 5. Accela 600 Pump operating specifications
Specification
Flow rate
a
Thermo Scientific
rangea
Description
1 to 5000 μL/min
Backpressure range
0 to 600 bar (8702 psi)
Residual pulsation
< 2 bar Amplitude
Flow rate accuracy
±0.5% or 1 μL/min, whichever is greater
Gradient composition accuracy
±0.5% absolute
Gradient resolution
1% from 0 to 100%
Firmware version (November 2010)
23.67 e
Gradient composition range
0 to 100% from up to 4 independent feed channels
employing 2 of them at the same time
Delay volume
90 μL (LDA) + 65 μL (static mixer)
The minimum flow rate for firmware versions earlier than 23.67 e is 10 μL/min.
Accela 600 and 1250 Pump Hardware Manual
7
1
Introduction
Specifications
Table 6. Accela 1250 Pump operating specifications
Specification
Description
Flow rate range
1 to 2000 μL/min
Backpressure range
0 to 1250 bar (18 130 psi)
Pressure signal accuracy
±1% absolute at calibration point
±5% full range
Residual pulsation
< 1.5 bar standard deviation full working range
Typical < 0.5 bar amplitude
Flow rate accuracy
±0.5% or 1 μL/min, whichever is greater
Gradient composition accuracy
±0.5% absolute from 1 to 1500 μL/min
± 2% absolute from 1500 to 2000 μL/min
Gradient resolution
1% from 0 to 100%
Firmware version (November 2010)
23.67 f
Gradient composition range
0 to 100% from up to 4 independent feed channels
employing 2 of them at the same time
Delay volume
70 μL (LDA) + 65 μL (static mixer)
Table 7. Degasser operating specifications
Specification
Description
Channels
4
Degassing technology
Gas permeation through a fluoropolymer
membrane
Maximum flow rate
10 mL/min
Degassing capacity
~2 ppm residual gas at 1 mL/min
Delay volume per channel
~480 μL
Wetted surfaces
PEEK, glass-filled PTFE, Teflon™ AF
Table 8. Electronic specifications
8
Specification
Description
Outputs
5 relays (4 programmable), 2 analog (1 for pressure)
Inputs
2 voltage actuated, 1 analog
Communications
19 200 baud, 1 start, 8 data, 1 stop, no parity and
no handshaking, proprietary protocol/USB 1.0
Audio output
Piezo buzzer
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
2
Accela 600 or 1250 Pump Installation
To install an Accela 600 or 1250 Pump as part of an Accela LC system, follow the “Accela
Pump Installation Checklist” on page 10 and the procedures in this chapter.
For information about installing hardware options, see Chapter 3, “Hardware Upgrades.”
Contents
• Accela Pump Installation Checklist
• Unpacking and Inspecting the Instrument
• Making the Initial Instrument Preparations
• Back Panel Connections
• Connecting the Low-Pressure Solvent Lines
• Connecting the Solvent Line Between the Pump and the Autosampler
• Powering On the Pump
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
9
2
Accela 600 or 1250 Pump Installation
Accela Pump Installation Checklist
Accela Pump Installation Checklist
When the Accela pump arrives at your facility, a Thermo Fisher Scientific field service
engineer performs the initial installation. When you move the pump from one location to
another, follow the steps in this installation checklist to ensure that the Accela pump operates
properly as part of an Accela LC system.
This installation checklist summarizes the steps that must be completed to properly install the
Accela pump:

Unpack and inspect your instrument (see “Unpacking and Inspecting the Instrument”
on page 11).

Read the safety precautions in “Safety Information” on page xvi.

Position the Accela pump appropriately on the benchtop (see “Making the Initial
Instrument Preparations” on page 11).

Make the back panel connections (see system installation procedures or “Connecting
the Pump to the Data System Computer” on page 17 and “Connecting the Accela
System Interconnect Cables” on page 17).

Connect the low-pressure solvent lines (see “Connecting the Low-Pressure Solvent
Lines” on page 24).

Connect the high-pressure solvent line between the Accela pump and the autosampler
(“Connecting the Solvent Line Between the Pump and the Autosampler” on page 27).

Connect the power cord and turn on the instrument (see “Connecting the Power
Cable” on page 16).

Add the Accela pump to the instrument configuration for the data system (see
Chapter 4).
This Accela pump was installed by:
(Name)
10
Accela 600 and 1250 Pump Hardware Manual
(Date)
Thermo Scientific
2
Accela 600 or 1250 Pump Installation
Unpacking and Inspecting the Instrument
Unpacking and Inspecting the Instrument
Carefully remove the Accela pump from the shipping container and inspect the pump and
packing for any signs of damage. If you find any damage, save the shipping materials and
immediately contact the shipping company.
Ensure that you have received all the items listed on the packing list. If any items are missing,
contact your Thermo Fisher Scientific field service engineer immediately.
Making the Initial Instrument Preparations
Place the Accela pump on a laboratory bench as close as possible to a designated electrical
outlet. Be sure to place the system in a draft-free location away from an open window, air
conditioner vents, or other circulating air source. A stable room temperature is necessary for
applications requiring maximum detection sensitivity. Choose an area that is free from dust,
moisture, direct sunlight, strong electromagnetic fields, and physical vibrations. Allow at least
15 cm (6 in.) of clear space between the back panel of the pump and any wall or obstruction.
This provides access to the back-panel connections and a free flow of cooling air.
Depending on the autosampler sampler model, follow these recommended benchtop layouts:
• Layout for an Accela LC System with an Accela Autosampler
• Layout for an Accela LC System with an Accela Open Autosampler
Layout for an Accela LC System with an Accela Autosampler
Figure 3 shows the recommended layout for a stand-alone Accela LC system with an Accela
Autosampler. The autosampler rests on top of the LC pump, followed by the detector and the
solvent platform. This setup requires a minimum benchtop area 38 cm (15 in.) wide by
51 cm (20 in.) deep. The height of the LC stack and a solvent platform with standard 1 liter
solvent bottles is 114 cm (45 in.). If you plan to use larger solvent containers, allow more
vertical space.
For safety reasons, limit the maximum height of the LC stack to 114 cm (45 in.). To
minimize the stack height for systems that include additional Accela instruments, follow these
guidelines:
• If your LC system includes two pumps, create a stack that includes the two pumps and
the solvent platform, and place the stack to the left of the autosampler.
• If your LC system includes an RI detector in addition to a UV/Vis detector or a PDA
detector, place the RI detector at the end of the solvent path to the right of the LC stack.
The minimum benchtop width for a system that includes an additional LC pump or an
additional detector is 94 cm (37 in.). The minimum benchtop width for a system that
includes an additional LC pump and an additional detector is 1.5 m (5 ft).
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
11
2
Accela 600 or 1250 Pump Installation
Making the Initial Instrument Preparations
Allow at least 15 cm (7 in.) of space between the system and any wall or obstruction. This
allowance provides access to the back-panel connectors and allows sufficient room for venting
the electronic components.
Figure 3.
Recommended layout for an LC system with an Accela Autosampler
ACCELA
PDA Detector
Power
Communication
Run
Lamps
Accela
Autosampler
ACCELA
Autosampler
Power
Communication
Run
Temperature
Power
Communication
Run
Degas
Ethernet cables
from the Accela Autosampler
and the Accela detector to the
Ethernet switch
114 cm
(45 in.)
ACCELA
Pump
76 cm
(30 in.)
USB cable
from the Accela pump
to the
data system computer
12
Accela 600 and 1250 Pump Hardware Manual
Electrical outlet
115 or 230 Vac
Thermo Scientific
2
Accela 600 or 1250 Pump Installation
Making the Initial Instrument Preparations
Layout for an Accela LC System with an Accela Open Autosampler
Figure 4 shows the recommended layout for a stand-alone Accela LC system with an Accela
Open Autosampler. This setup requires a minimum benchtop area 68 cm (27 in.) wide by
72 cm (28 in.) deep. The LC pump and detector are stacked below the autosampler table.
The height of the Accela Open Autosampler with attached table is 104.5 cm (41 in.).
For Accela LC systems that include additional Accela instruments, follow these guidelines:
• If your LC system includes two LC pumps and a detector, stack the pumps under the
autosampler table and place the detector to the side of the table.
• If your LC system includes two pumps and two detectors, place the pump stack under the
autosampler table and the detector stack to the side of the table. If your LC system
includes an RI detector in addition to a UV/Vis detector or a PDA detector, place the RI
detector at the end of the solvent path.
The minimum benchtop width for a system that includes additional Accela modules is
1.2 m (4 ft).
Allow at least 25 cm (10 in.) of space between the system and any wall or obstruction. This
allowance provides access to the back-panel connectors and allows sufficient room for venting
the electronic components. The solvent bottle tray is located behind the injection valve and is
bolted to the autosampler table.
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
13
2
Accela 600 or 1250 Pump Installation
Making the Initial Instrument Preparations
Figure 4.
Recommended layout for an Accela LC system with an Accela Open Autosampler
Width
68 cm
(27 in.)
Accela Open Autosampler
RS-232 connection
to the data system
computer
SER1
LAN
MODBUS
BUZZER L
E
D
INTERFACE 1
WASH
STATION
INTERFACE 2
AUX 1
AUX 2
POWER FUSE
AUX
TERMINAL
Height
104.5 cm
(41 in.)
STACK COOLER DW
0 1
9
9
7 8
230V
7 8
115V
T3.15A/250V
ENET
GND B
CHB - 1V
CHB - 100mV
CHB - 10mV
GND A
CHA - 1V
CHA - 100mV
CHA - 10mV
DISCONNECT INPUT
REFER ALL SERVICE
POWER BEFORE
Accela PDA 80 Hz Detector
100V
UNIT ID
CAUTION
355 River Oaks Parkway
San Jose, CA 95134, U.S.A
Model:
TO QUALIFIED
OPENING COVER.
Serial No:
940XXX
Part No:
60057-60141
Option/Firmware 5cm Lightpipe / 3.00
LINE
FUSE
5 6
5 6
EVENT
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
Power supply
and control module
for the stack cooler
4
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
Autosampler
table
2 3
4
0 1
2 3
RS232
PERSONNEL ONLY
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
(1) This device may not cause harmful interference,
and (2) This device must accept any interference
received, including interference that may cause
undesired operation.
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
5, 608, 517
61010 1
50/60 HZ
T1.6A/250V
Detector
USE ONLY WITH
250 V FUSES
100VA
DISCONNECT POWER
BEFORE REPLACING FUSES
!
355 River Oaks Parkway
San Jose, CA, U.S.A
Input: 100V – 240V
2A
50/60 HZ
Output: 24V
4.5A
CAUTION
DISCONNECT INPUT
POWER BEFORE
!
OPENING COVER.
Model: 1250 Pump
I
REFER ALL SERVICE
TO QUALIFIED
PERSONNEL ONLY.
EN
61010 1
Serial No: 925000
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
(1) This device may not cause harmful interference,
and (2) This device must accept any interference
received, including interference that may cause
undesired operation.
RS232
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
5,653,876
6,248,157 B1 EP 2107241A2
Futher patent pending
!
DO NOT USE
TOGETHER
WITH
D-SUB25
1 2 3 4 5 6 7 8
USB
RESERVED
GND
INJECT HOLD
PUMP READY
GRAD. START
RESERVED
5V
GND
PELTIER
THERMOSTAT
D-SUB25
DO NOT COVER
O
LINE
FUSE
115V
T2.0A
230V
T1.0A
50/60 HZ
220VA
ETL LISTED
CONFORMS TO
UL STD 61010A-1
CERTIFIED TO
CAN/USA C22.2 STD NO. 61010-1
Accela
pump
I
0
100V – 240 V ~ 4A 50/60 HZ
!
100V
36V
PAL
4.16A
AUX
Power supply module
for the autosampler
(front and back)
76 cm
(30 in.)
Electrical outlet
115 V or 230 Vac
14
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
2
Accela 600 or 1250 Pump Installation
Back Panel Connections
Back Panel Connections
To operate the Accela pump from the data system computer, connect the power cable and the
USB communication cable. To operate the Accela pump as part of an Accela LC system,
connect the interconnect cable that coordinates the operation of the pump with the other
Accela modules. Figure 5 shows the back panel of the Accela 1250 Pump.
CAUTION Never connect the Accela pump to an electrical line source that has power
drops or fluctuations of 10% below the nominal rated line voltage.
Figure 5.
Back panel of the Accela 1250 Pump
Interconnect cable socket
for a system with an
Accela Autosampler
RS-232
connector
USB series B socket
!
355 River Oaks Parkway
San Jose, CA, U.S.A
REFER ALL SERVICE
POWER BEFORE
TO QUALIFIED
OPENING COVER.
PERSONNEL ONLY
This device complies with FCC Rules, Part 15.
Operation is subject to the following conditions
(1) This device may not cause harmful interference,
and (2) This device must accept any interference
received, including interference that may cause
undesired operation.
5,653,876
Serial No: 925000
RS232
This product is manufactured under, and covered
by, one or more of the following U.S. patents:
6,248,157 B1 EP 2107241A2
Futher patent pending
!
1 2 3 4 5 6 7 8
USB
RESERVED
GND
INJECT HOLD
PUMP READY
GRAD. START
RESERVED
5V
GND
Model: 1250 Pump
CAUTION
DISCONNECT INPUT
DO NOT USE
TOGETHER
WITH
D-SUB25
D-SUB25
DO NOT COVER
Line
Fuse
100V - 240V
50/60 Hz
2x T2.0A/250V
220VA
Power line
receptacle
D-sub 25-pin connector
(accepts the start signal
from an Accela Open
Autosampler or a CTC
autosampler)
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
15
2
Accela 600 or 1250 Pump Installation
Back Panel Connections
To make the back panel connections, follow these procedures:
• Connecting the Power Cable
• Connecting the Pump to the Data System Computer
• Connecting the Accela System Interconnect Cables
• Additional Hardware Configurations
Connecting the Power Cable
The Accela LC modules ship with power cords appropriate to their shipping destination
(see Table 9). Local codes in your area might require that you install another type of plug and
receptacle. Your local Thermo Fisher Scientific field service engineer will provide the
appropriate power plugs.
Table 9. Power cords supplied according to shipping destination
Destination
Plug type
Voltage
rating
Current
rating
P/N
United Kingdom
BS 1363
250 Vac
5A
6003-0810
Switzerland
SEV 1011
250 Vac
10 A
6003-0620
Europe
CEE 7/7
250 Vac
10 A
6003-0330
United States and Canada
NEMA
5-15P
125 Vac
10 A
6003-0160
China
C13
250 Vac
10 A
00302-99-00026
The NEMA 5-15P plug and its corresponding receptacle are shown in Figure 6.
Figure 6.
NEMA 5-15P power plug and receptacle
Receptacle
Plug
 To connect the pump to line power
1. Connect the power cord to the line power receptacle on the back panel of the Accela
pump (see Figure 5 on page 15).
2. Connect the power cord to an appropriate line power source.
16
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
2
Accela 600 or 1250 Pump Installation
Back Panel Connections
Connecting the Pump to the Data System Computer
The Accela pump communicates with the data system computer through a USB connection.
 To connect the pump to the computer with a USB cable
1. Connect the series A plug (see Figure 7) of the USB cable to an available USB port on the
computer.
Figure 7.
USB plugs and sockets
Series A socket
Series B socket
Series A plug
Series B plug
2. Connect the series B plug (see Figure 7) of the USB cable to the USB port on the back of
the Accela pump.
Connecting the Accela System Interconnect Cables
An Accela LC system can include an Accela Autosampler or an Accela Open Autosampler1,
one or two Accela pumps, and one to three LC detectors.
The contact closure connections depend on the autosampler model. For information about
making the contact closure connections for your system, see one of these topics:
• Contact Closure Connections for Accela Systems with an Accela Autosampler
• Contact Closure Connections for Accela Systems with an Accela Open Autosampler
1
Thermo Scientific
You can control the Accela Open Autosampler from the ChromQuest 5.0 data system or the Xcalibur 2.1.x data
system or later.
Accela 600 and 1250 Pump Hardware Manual
17
2
Accela 600 or 1250 Pump Installation
Back Panel Connections
Contact Closure Connections for Accela Systems with an Accela Autosampler
The system interconnect cable synchronizes the timing of the Accela devices. The
interconnect cable has seven combicon connectors: three are labeled DETECTOR; two are
labeled PUMP; one has a small, A/S tag on its adjacent cable; and one has a small, M/S tag on
its adjacent cable (see Figure 8). Figure 9 on page 19 shows the connections to the system
modules.
Figure 8.
System interconnect cable with seven combicon connectors
DETECTOR
DETECTOR
DETECTOR
M/S
PUMP
Autosampler
connector
PUMP
A/S
 To connect the system interconnect cable
1. Plug one of the pump connectors with a yellow PUMP sticker into the 8-pin socket on
the back panel of the Accela pump (all models).
Table 10 lists the pin out descriptions for the 8-pin socket.
Table 10. Accela 600 Pump interconnect cable socket pin out descriptions
Pin
Description
Pin
Description
1
GND
5
Pump ready
2
5V
6
Inject hold
3
Reserved
7
GND
4
Grad start
8
Reserved
2. Plug the autosampler connector with the A/S tag on its adjacent cable into the left, 8-pin
socket on the back panel of the Accela Autosampler.
3. If your LC system contains an Accela PDA Detector, an Accela UV/Vis Detector, or an
Accela RI Detector, plug one of the detector connectors with a blue DETECTOR sticker
into the left, 8-pin socket on the back panel of the detector.
Your Accela LC system can contain one or two pumps and up to three detectors.
18
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
2
Figure 9.
Accela 600 or 1250 Pump Installation
Back Panel Connections
System interconnect cable connections for the Accela LC system
Connector labeled
DETECTOR
Connector labeled
DETECTOR
9
5 6
7 8
9
4
5 6
0 1
2 3
4
0 1
2 3
9 10 11 12 13 14 15 16
PURGE
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
NC
CHASSIS GND
INTEGRATOR -INTEGRATOR +
NC
CHASSIS GND
RECORDER -RECORDER +
7 8
Accela PDA Detector or Accela UV/Vis Detector
1 2 3 4 5 6 7 8
ENET
Accela RI Detector
System interconnect
cable
9
5 6
7 8
9
4
5 6
0 1
2 3
4
0 1
2 3
7 8
Accela Autosampler
Connector labeled
PUMP
!
RS232
DO NOT USE
TOGETHER
WITH
D-SUB25
1 2 3 4 5 6 7 8
USB
RESERVED
GND
INJECT HOLD
PUMP READY
GRAD. START
RESERVED
5V
GND
Accela 600 Pump or Accela 1250 Pump
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
19
2
Accela 600 or 1250 Pump Installation
Back Panel Connections
Contact Closure Connections for Accela Systems with an Accela Open Autosampler
The contact closure cable for an Accela LC system with an Accela Open Autosampler is
provided with the Accela Open Autosampler. LC/MS systems that include a TSQ™ Series or
Exactive™ mass spectrometer or an Accela detector require an additional adapter cable, which
is also supplied with the Accela Open Autosampler.
Figure 10 shows the contact closure cable for an Accela LC system with an Accela Open
Autosampler and one or two Accela 600 Pumps, Accela 1250 Pumps, or a combination of
these pumps.
Tip An unlabeled cable equivalent to the Accela Open Autosampler interconnect cable
(P/N 60157-63024) ships with the autosampler.
This unlabeled cable has three connectors:
• The DB15 connector plugs into the Interface 1 port on the back panel of the
autosampler.
• The DB25 connector plugs into the D-Sub 25 port on the back panel of an
Accela 600 or 1250 Pump. For a dual-pump system, plug this connector into the
eluting pump’s D-Sub 25 port. Do not connect a contact closure cable to the loading
pump.
• The 2-socket connector connects to the Start In pins of an MSQ™ or LTQ™ Series
mass spectrometer.
Figure 10. Accela Open Autosampler interconnect cable (P/N 60157-63024)
TO PUMP
60157-63024
TO A/S
TO MSQ/LTQ/LXQ
Figure 11 shows the adapter cable for an Accela LC system with an Accela Open Autosampler
and an Accela detector (refractive index, UV/Vis, or photodiode array detector) or TSQ Series
or Exactive mass spectrometer.
20
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
2
Accela 600 or 1250 Pump Installation
Back Panel Connections
Figure 11. Adapter cable for an Accela detector (P/N 60157-63026)
TO 60157-63024
60157-63026
TO LCQ FLEET
TO LTQ/LXQ/MSQ
TO PDA
TO TSQ/EXACTIVE
 To connect the contact closure cables to the Accela LC system modules
1. Connect the Accela Open Autosampler interconnect cable (P/N 60157-63024) to the
Accela Open Autosampler and the Accela 600 or 1250 Pump as follows:
a. Plug the DB15 connector into the Interface 1 receptacle on the back panel of the
Accela Open Autosampler.
b. Plug the 25-pin connector into the D-Sub25 receptacle on the back panel of the
Accela 600 or 1250 pump.
Tip For a dual-pump system, connect the 25-pin connector to the eluting pump.
Do not connect a contact closure cable to the loading pump.
For information about setting up the configuration for the device drivers, see
Chapter 4, “Instrument Driver Configuration.” For information about setting
up the trigger signal for the loading pump (typically pump 2) and the pump
programs for both pumps, refer to the Accela user guide for your data system.
2. Connect the 2-pin connector labeled “TO 60157-63024” on the adapter cable to the
2-pin connector labeled “TO MSQ/LTQ/LXQ” on the Accela Open Autosampler
interconnect cable.
3. Connect the adapter cable to pins 1 through 8 on the back panel of the Accela detector.
Figure 12 shows the contact closure connections for an Accela LC system with an Accela
Open Autosampler, Accela 600 or 1250 Pump, and Accela detector.
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
21
2
Accela 600 or 1250 Pump Installation
Back Panel Connections
Figure 12. Contact closure connections for an Accela LC system with an Accela Open Autosampler
Accela Open Autosampler
INTERFACE 1
Connection between the
Accela Open Autosampler interconnect cable
and the detector adapter cable
TO 60157-63024
60157-63026
TO LCQ FLEET
TO LTQ/LXQ/MSQ
TO A/S
TO MSQ/LTQ/LXQ
TO PDA
60157-63024
TO TSQ/EXACTIVE
PDA detector or UV/Vis detector
RI Detector
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
ENET
NC
CHASSIS GND
INTEGRATOR -INTEGRATOR +
NC
CHASSIS GND
RECORDER -RECORDER +
GND B
CHB - 1V
CHB - 100mV
CHB - 10mV
GND A
CHA - 1V
CHA - 100mV
CHA - 10mV
UNIT ID
PURGE
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
EVENT
GND
ZERO
READY
RUN
NC
+5V @ 150mA
GND
RS232
ENET
Accela 600 or 1250 Pump
D-SUB25
Tip
For a dual-pump system, plug this connector into the
eluting pump. Do not connect a contact closure cable
to the loading pump.
TO PUMP
22
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
2
Accela 600 or 1250 Pump Installation
Back Panel Connections
Additional Hardware Configurations
Use the D-sub 25-pin connector on the back panel of the Accela pump to connect the pump
to a CTC autosampler or a valve interface module.
IMPORTANT Never use both the D-sub 25 pin connector and the pump interconnect
connector (pins 1 through 8).
Table 11 lists the pin out descriptions for the D-sub 25-pin connector.
Table 11. D-sub 25 pin connector pin out descriptions
Thermo Scientific
Pin
Description
Pin
Description
Pin
Description
1
Rel. 1 Input
10
Dig. In2+
19
Rel. 4 NC
2
Rel. 1 NC
11
Rel. Error In.
20
GND
3
Rel. 2 NO
12
Rel. Error NC
21
GND
4
Rel. 3 Input
13
GND
22
Dig. In1-
5
Rel. 3 NC
14
Rel. 1 NO
23
Dig. In2-
6
Rel. 4 NO
15
Rel. 2 Input
24
Rel. Error NO
7
Pressure Out
16
Rel. 2 NC
25
+5 Vdc
8
+24 Vdc
17
Rel. 3 NO
9
Dig. In1+
18
Rel. 4 Input
Accela 600 and 1250 Pump Hardware Manual
23
2
Accela 600 or 1250 Pump Installation
Connecting the Low-Pressure Solvent Lines
Connecting the Low-Pressure Solvent Lines
The Accela 600 Pump Inlet Tubing Kit contains preassembled, low-pressure solvent lines that
have a 20 μm particle size, stainless steel filter at one end and a flangeless fitting at the other
end (see Figure 13).
To install the solvent lines, follow these procedures:
• Assembling the Solvent Reservoir Bottles
• Connecting the Solvent Reservoir Bottles to the Degassing Unit
• Priming the Degassing Membranes
Assembling the Solvent Reservoir Bottles
 To assemble the solvent reservoir bottles
1. Label one of the four solvent reservoir bottle caps “A.”
2. Pull the filter off the end of the solvent line, and then pass the tubing through the solvent
reservoir bottle cap labeled A.
3. Reconnect the filter to the end of the solvent line (see Figure 13).
IMPORTANT
Only use stainless steel, 20 μm particle size sinker frits to terminate
the solvent reservoir lines.
Figure 13. Solvent reservoir cap assembly with the solvent line assembly
Solvent reservoir
bottle cap
1/4-28 PEEK nut and
Tefzel ferrule
24
Accela 600 and 1250 Pump Hardware Manual
Solvent reservoir
filter
(20 μm particle size,
stainless steel)
Thermo Scientific
2
Accela 600 or 1250 Pump Installation
Connecting the Low-Pressure Solvent Lines
4. Insert the solvent reservoir filter and inlet line into one of the solvent reservoir bottles,
and screw the cap onto the solvent reservoir bottle until it is secure.
Tip The cap is a two-piece assembly. The upper section (see Figure 13) snaps onto a
threaded section. You can screw the threaded section onto the bottle and snap on the
upper section after installing the tube, or, if you are replacing existing tubing, you can
unscrew the entire cap from the bottle. You might also be able to use the upper section
alone for solvent bottles with a wider neck.
5. Position the bottle in the solvent platform or tray, allowing the solvent inlet line to hang
down along the left side of the system.
• For an LC system with an Accela Autosampler, the solvent reservoir bottles reside in
the solvent platform at the top of the system.
• For an LC system with an Accela Open Autosampler, the solvent reservoir bottles
reside in the solvent tray attached to the autosampler table.
6. Repeat step 1 through step 5 for solvents B, C, and D if applicable.
Connecting the Solvent Reservoir Bottles to the Degassing Unit
Use HPLC-grade solvents that are free of particulate matter and terminate the solvent
reservoir lines with the stainless steel, 20 μm particle size sinker frit provided in the
Accela 600 Pump Inlet Tubing Kit. The Accela 600 Pump Inlet Tubing Kit ships with both
the Accela 600 Pump and the Accela 1250 Pump.
CAUTION Do not use solvents containing Freon™ and perfluorinated solvents, such as
Fluorinert™ and Fomblin™ perfluoro polyether solvents. They adversely affect the Teflon
AF degassing membrane.
CAUTION To prevent personal injury, observe good laboratory practice when handling
solvents, changing tube lines, or both. Consult the pertinent material safety data sheets for
the solvents used for HPLC analysis.
 To connect the solvent reservoir bottles to the degassing unit
1. Route the solvent reservoir lines through the access slots on the top-left side of the
module enclosures and the bottom-left sides of the module drip trays.
2. For each solvent reservoir line, connect the flangeless fitting to the appropriate inlet port
of the built-in degassing unit.
Figure 14 shows the flangeless fitting connected to the solvent reservoir line. The
flangeless fittings come in four colors: blue, green, yellow, and orange.
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
25
2
Accela 600 or 1250 Pump Installation
Connecting the Low-Pressure Solvent Lines
Figure 14. Low-pressure solvent line with flangeless fitting
Figure 15 shows the inlet ports of the built-in degassing unit and the access slots in the
module enclosure and drip tray.
Figure 15. Solvent reservoir line connections to the inlet ports of the vacuum degasser
1 liter
solvent reservoir
bottles
A
Enclosure
access slot
B
C
Solvent platform
(for an LC system with an
Accela Autosampler)
Solvent reservoir line
connections to the
degassing unit
Drip tray
access slot
26
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
2 Accela 600 or 1250 Pump Installation
Connecting the Solvent Line Between the Pump and the Autosampler
Priming the Degassing Membranes
Prime the degassing membranes as part of the initial Accela pump installation procedure.
CAUTION To avoid damaging the degassing unit and the piston seals, prime the degassing
membranes by using a syringe to pull solvent through the solvent lines into the degassing
unit.
Do not push the solvent into the degasser. Pushing the solvent can generate excessive
pressure and rupture the degassing membranes. The maximum recommended pressure on
the membrane is 10 bar.
Do not use the pump to prime the solvent lines and the degassing membranes. Drawing
excessive amounts of air through the liquid displacement assembly can damage the piston
seals.
 To prime the degassing membranes
1. Remove the tubing connected to inlet valve A.
2. Connect a 10 mL syringe to the end of the tubing you removed in step 1.
3. Draw approximately 5 mL solvent into the syringe.
4. Disconnect the syringe and reconnect the tubing to inlet valve A.
5. Repeat step 1 through step 4 to prime solvents B, C, and D through the degasser if
applicable.
Connecting the Solvent Line Between the Pump and the Autosampler
To connect the high-pressure solvent line between the pump and the autosampler, follow
these procedures:
• Connecting the High-Pressure Solvent Line to the Pump Outlet
• Connecting the High-Pressure Solvent Line to the Autosampler
IMPORTANT Avoid using PEEK tubing and fittings with system pressures above
276 bar (4000 psi). Thermo Fisher Scientific ships precut 1/16 in. OD, stainless steel
tubing and two-piece, stainless steel compression fittings with the Accela autosamplers.
CAUTION To prevent personal injury caused by skin contact with hazardous solvents,
turn off the pump flow before you connect the high-pressure solvent line between the LC
pump and the autosampler.
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
27
2
Accela 600 or 1250 Pump Installation
Connecting the Solvent Line Between the Pump and the Autosampler
Connecting the High-Pressure Solvent Line to the Pump Outlet
When you operate the Accela pump at pressures above 276 bar (4000 psi), use the supplied
precut, stainless steel tubing (0.005 in. ID × 1/16 in. OD) to connect the pump to the
autosampler:
• For the Accela Autosampler, use the 26 cm (10 in.) length of precut tubing in the Accela
System Kit.
• For the Accela Open Autosampler, use the 0.6 m (2 ft.) length of precut tubing in the
Accela Open Autosampler Accessory Kit.
Tip To identify the tubing ID, the precut tubing has a color-coded band:
• Red = 0.005 in.
• Black = 0.007 in.
• Blue = 0.010 in.
• Yellow = 0.020 in.
 To connect the high-pressure solvent line to the LC pump outlet
1. Turn off the pump flow.
2. Using the supplied two-piece, compression fitting, connect the precut, stainless steel
tubing to the pump outlet (dynamic mixer outlet [see Figure 16] or placeholder blind
module outlet).
Figure 16. Dynamic mixer outlet
Dynamic
mixer
outlet
28
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
2 Accela 600 or 1250 Pump Installation
Connecting the Solvent Line Between the Pump and the Autosampler
Figure 17 shows the two-piece, compression fitting. The length of the nut is 0.45 inches,
and the length of the ferrule is 0.188 inches.
Figure 17. Two-piece compression fitting (stainless steel)
1/16 in. OD
stainless steel
tubing
Nut
Ferrule
0.188 in.
0.45 in.
3. Using a 1/4 in. open-ended wrench, tighten the nut.
Connecting the High-Pressure Solvent Line to the Autosampler
The Accela family of liquid chromatography instruments includes two autosamplers: the
Accela Autosampler and the Accela Open Autosampler.
To connect the high-pressure solvent line, follow the procedure for your autosampler:
• Connecting the Solvent Line to the Accela Autosampler Heat Exchanger
• Connecting the Solvent Line to the Accela Open Autosampler Injection Valve
Connecting the Solvent Line to the Accela Autosampler Heat Exchanger
The metal plate in front of the Accela Autosampler heat exchanger can reach temperatures as
high as 95 °C (203 °F).
CAUTION To prevent personal injury, before installing the LC column or connecting the
pump to the autosampler, ensure that the column oven compartment is at room
temperature. The column oven compartment can reach temperatures as high as 95 °C
(203 °F).
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
29
2
Accela 600 or 1250 Pump Installation
Connecting the Solvent Line Between the Pump and the Autosampler
Figure 18 shows the Hot Surface caution symbols on the column oven surface.
Figure 18. Hot surface cautions on the surface of the Accela Autosampler column oven
Hot surface caution
symbols
 To connect the high-pressure solvent line to the heat exchanger inlet
1. Ensure that the column oven compartment is at room temperature and that the pump
flow is turned off.
2. Route the high-pressure tubing through the access slot in the autosampler drip tray.
3. Connect the tubing to the heat exchanger inlet as follows:
a. Using a two-piece, compression fitting (see Figure 17 on page 29), connect the
tubing to the heat exchanger inlet.
b. Using a 1/4 in. open-ended wrench, tighten the nut.
Figure 19 shows the high-pressure connection between the Accela Autosampler and the
Accela 600 or 1250 Pump.
30
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
2 Accela 600 or 1250 Pump Installation
Connecting the Solvent Line Between the Pump and the Autosampler
Figure 19. Connection between the Accela 600 or Accela 1250 Pump and the Accela Autosampler
20
Connection to the
heat exchanger inlet
µL
3
4
2
5
1
6
Access slot in the
autosampler drip tray
Dynamic
mixer
-orPlaceholder
module
Static mixer
Connecting the Solvent Line to the Accela Open Autosampler Injection Valve
The Accela Open Autosampler has a high-pressure Valco™ injection valve (18 500 psi). To
make connections to this valve, use high-pressure compression fittings and the 0.6 m (2 ft),
precut stainless steel tubing in the Accela Open Autosampler Accessory Kit.
 To connect the high-pressure solvent line to the injection valve
1. Route the stainless steel tubing connected to the LC pump outlet through the cutout on
the top of the pump enclosure behind the right door, through the access slot in the
detector drip tray, and through the cutout on the top of the detector enclosure behind the
right door.
2. Using a two-piece, compression fitting (see Figure 17 on page 29), connect the tubing to
port 4 of the injection valve (see Figure 20).
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Accela 600 or 1250 Pump Installation
Powering On the Pump
Figure 20. Accela Open Autosampler injection valve and wash station
Injection port
of the injection valve
Wash
station
2
6
3
1
5
High-pressure
solvent line connection
to the LC column
4
High-pressure
solvent line connection
to the LC pump
3. Using a 1/4 in. open-end wrench, tighten the fitting.
4. Turn on the pump flow and check for solvent leaks at the pump outlet and the
autosampler inlet.
Powering On the Pump
 To turn on the pump
1. Verify that the pump’s power switch is in the Off position.
2. Connect the power cord (see “Connecting the Power Cable” on page 16).
3. Connect the pump to the computer (see “Connecting the Pump to the Data System
Computer” on page 17).
4. Connect the solvent lines (see “Connecting the Low-Pressure Solvent Lines” on page 24).
5. Connect the high-pressure solvent line between the pump and the autosampler (see
“Connecting the Solvent Line Between the Pump and the Autosampler” on page 27).
6. Press the power On button.
The Power LED turns solid green.
To control the pump from the data system computer, add the pump to the data system
configuration (see Chapter 4, “Instrument Driver Configuration,” on page 59).
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Hardware Upgrades
You can order these hardware options from Thermo Fisher Scientific for your Accela or
Transcend pump: a dynamic mixer, a seal wash pump, and a leak sensor.
Note Because the installation involves the removal of the pump chassis from the pump
enclosure, Thermo Fisher Scientific recommends that you have a Thermo Fisher Scientific
field service engineer install these hardware options.
To install the optional seal wash pump, dynamic mixer, or leak sensor, follow these
procedures.
Contents
• Dynamic Mixer Installation
• Seal Wash Pump Installation
• Leak Sensor Installation
• Setting Up Continuous Flushing
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Dynamic Mixer Installation
Dynamic Mixer Installation
For high-pressure gradient applications, Thermo Fisher Scientific recommends that you
install the optional 35 μL dynamic mixer (see Figure 21). To install the dynamic mixer, refer
to the product insert provided with the mixer or the following instructions.
Figure 21. Dynamic mixer inlet and outlet ports
Dynamic mixer
inlet port
Dynamic mixer
outlet port
to autosampler
To install the dynamic mixer, you must have these tools:
Tool
Use
1/4 in. open-end wrench
To disconnect the high-pressure lines
#2 Phillips head screwdriver
To disconnect the chassis from the enclosure
2.5 mm ball driver wrench
To disconnect the screws that secure the blind outlet
(placeholder) module or the dynamic mixer to the front
panel of the pump
To install the dynamic mixer, follow these steps:
1. Removing the Pump Chassis from the Enclosure
2. Connecting the Dynamic Mixer to the Pump’s Front Panel
3. Connecting the Dynamic Mixer to the Controller Board
4. Reconnecting the Static Mixer and the High-Pressure Lines
5. Reconnecting the Pump Chassis to the Enclosure
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Dynamic Mixer Installation
Removing the Pump Chassis from the Enclosure
 To remove the pump chassis from the enclosure
1. Turn off the power to the LC pump.
2. Unplug the pump from line power.
CAUTION To prevent electrical shock, turn off the pump and disconnect it from line
power before you pull the pump chassis out of the enclosure.
3. Using a 1/4 in. open-end wrench, disconnect the static mixer from the blind outlet
(placeholder) module (see Figure 22 and Figure 23).
Figure 22. Static mixer connection to the blind outlet module
Static mixer connection to
the blind outlet module
Blind outlet
(placeholder)
module
4. If the high-pressure tubing is connected between the pump and the autosampler, using a
1/4 in. open-end wrench, disconnect the high-pressure solvent line from the blind outlet
module.
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Dynamic Mixer Installation
Figure 23. Static mixer disconnected from the blind outlet (placeholder) module
Static mixer
5. Using a #2 Phillips head screwdriver, remove the screw that secures the metal plate
covering the LED board, and then remove the metal plate (see Figure 24 and Figure 25).
Figure 24. Front view of the pump with the doors open
Screw that secures the
metal plate
Four screws that secure the enclosure
to the chassis
6. Disconnect the LED cable from the left door of the pump (see Figure 25).
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Figure 25. LED cable connection
LED cable connection to the pump’s left door
7. Using a #2 Phillips head screwdriver, remove the four screws that secure the pump chassis
to the enclosure (see Figure 24 on page 36), and then pull the chassis out of the enclosure.
Go to the next procedure, “Connecting the Dynamic Mixer to the Pump’s Front Panel.”
Connecting the Dynamic Mixer to the Pump’s Front Panel
 To connect the dynamic mixer to the front panel of the pump
1. Using a 2.5 mm hex ball driver, remove the two screws (with washers) that secure the
blind outlet (placeholder) module to the front panel of the pump (see Figure 26). Set the
screws and washers aside. You use them in step 5 to secure the dynamic mixer to the
pump.
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Dynamic Mixer Installation
Figure 26. Back side of the pump’s front panel, showing the screws (with washers) being
removed
Screws that secure the
blind outlet (placeholder) module
to the front panel of the pump
2. Remove the blind outlet (placeholder) module from the front panel of the pump
(see Figure 27).
Figure 27. Blind outlet module removed from the front panel
Blind outlet module
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Dynamic Mixer Installation
3. Using a 1/4 in. open-end wrench, disconnect the static mixer from the LDA
(see Figure 28 and Figure 29).
Figure 28. Static mixer connection to the LDA
Connection between LDA and
static mixer
Figure 29. Static mixer disconnected from the pump
Static mixer
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Dynamic Mixer Installation
4. Insert the dynamic mixer into the port vacated by the blind outlet (placeholder) module
(see Figure 30).
Figure 30. Dynamic mixer inserted into the port vacated by the blind outlet module
Dynamic mixer
5. From the back side of the front panel (see Figure 31), secure the dynamic mixer with the
screws that you removed in step 1 on page 37.
Note Figure 31 and Figure 32 do not show the cable portion of the dynamic mixer.
Figure 31. Dynamic mixer, as viewed from the inside of the pump chassis
Back side of
the dynamic
mixer
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Dynamic Mixer Installation
6. Using a 2.5 mm hex ball driver, tighten the screws that secure the dynamic mixer to the
pump (see Figure 32).
Figure 32. Dynamic mixer secured to the pump chassis
Screws that secure the dynamic mixer
to the pump
Go to the next procedure, “Connecting the Dynamic Mixer to the Controller Board.”
Connecting the Dynamic Mixer to the Controller Board
 To connect the dynamic mixer to the Controller board
Connect the dynamic mixer’s cable to the P3 cable (see Figure 33). One end of the P3
cable is connected to the Controller board and the other end is free inside the pump
chassis.
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Dynamic Mixer Installation
Figure 33. Dynamic mixer connection to the P3 cable
Dynamic mixer
cable
P3
cable
Go to the next procedure, “Reconnecting the Pump Chassis to the Enclosure.”
Reconnecting the Pump Chassis to the Enclosure
 To reconnect the pump chassis to the enclosure
1. Push the pump chassis back into the enclosure, and then secure the chassis to the
enclosure with the four screws that you removed in step 7 on page 37.
2. Reconnect the LED cable to the LED board.
3. Align the hole in the LED cover plate with the port in the door for the screw. Slide the
screw through the hole. Using a #2 Phillips head screwdriver, tighten the screw to secure
the metal plate to the left door of the pump.
Go to the next procedure, “Reconnecting the Static Mixer and the High-Pressure Lines.”
Reconnecting the Static Mixer and the High-Pressure Lines
 To reconnect the static mixer and the high-pressure lines
1. Reinstall the static mixer as follows:
a. Using a 1/4 in. open-end wrench, reconnect the static mixer to the LDA.
b. Using a 1/4 in. open-end wrench, connect the static mixer to the inlet of the dynamic
mixer (see Figure 34).
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Figure 34. Static mixer connected to the LDA and the dynamic mixer
LDA outlet
Static mixer
Dynamic mixer inlet
2. Connect the outlet of the dynamic mixer to the autosampler inlet (see “Connecting the
Solvent Line Between the Pump and the Autosampler” on page 27).
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Seal Wash Pump Installation
Seal Wash Pump Installation
If your applications use highly buffered mobile phases, Thermo Fisher Scientific recommends
installing the optional seal wash pump.
To install the optional seal wash pump, you must have these tools:
Tool
Use
#2 Phillips head screwdriver
To disconnect the LED cover plate from the inside of the
pump’s left door and the chassis from the enclosure
2.5 mm hex ball driver
To disconnect the screws that secure the cover plate to the
front panel and secure the seal wash pump to the front
panel
 To install the optional seal wash pump
1. Turn off the power to the pump.
2. Unplug the pump from line power.
CAUTION To prevent electrical shock, turn off the pump and disconnect it from line
power before you install the seal wash pump.
3. Using a 2.5 mm hex ball driver, remove the two screws that secure the blind cover to the
front panel of the pump (see Figure 35). Set the screws aside. You will use these screws in
step 5 on page 45 to secure the seal wash pump to the front panel of the pump.
Figure 35. Blind cover and screws removed from the pump’s front panel
Blind cover
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Screws that secure the blind cover
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3 Hardware Upgrades
Seal Wash Pump Installation
4. With the cable connector facing up (see Figure 36), slide the seal wash pump into the
front panel of the pump (see Figure 37).
Figure 36. Seal wash pump
Cable connector
Figure 37. Seal wash pump placed into the front panel of the pump
Seal wash pump
5. Using the two screws that you removed in step 3 on page 44, secure the seal wash pump
to the front panel of the pump (see Figure 38).
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Seal Wash Pump Installation
Figure 38. Seal wash pump secured to the front panel of the pump
Screws that secure the seal wash pump
6. Using a 2.5 mm hex ball driver, tighten the screws.
7. Remove the pump chassis from the enclosure (see “Removing the Pump Chassis from the
Enclosure” on page 35).
CAUTION Observe precautions for handling electrostatic sensitive devices.
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Seal Wash Pump Installation
8. Using the cable supplied with the seal wash pump, connect the seal wash pump to Valve E
on the Controller board (see Figure 39).
Figure 39. Seal wash pump connection to the Controller board
Cable connection to
seal wash pump
Cable connection to
Controller board
9. Reconnect the pump chassis to the enclosure (see “Reconnecting the Pump Chassis to the
Enclosure” on page 42).
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Seal Wash Pump Installation
10. Remove the inner wash line from the guide bushings (see Figure 40).
Figure 40. Guide bushings with the inner wash line removed
Inner wash line removed
11. To set up the seal wash pump for continuous flushing, go to “Setting Up Continuous
Flushing” on page 57.
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Leak Sensor Installation
Leak Sensor Installation
Thermo Fisher Scientific recommends installing the optional leak sensor to stop the solvent
flow from the pump when a leak occurs.
The Leak Sensor Kit (P/N 00960-01-00024) contains these items:
• Leak sensor with attached cable
• Mounting bracket
• Locating fixture
• Two-component adhesive
 To install the optional leak sensor
1. Turn off the power to the pump and unplug the pump from line power.
2. Glue the mounting bracket for the leak sensor to the pump’s drip tray as follows:
a. Place the locating fixture against the front panel of the pump as shown in Figure 41.
Figure 41. Appropriate position for the locating fixture
Locating fixture
b. Mix the two-component adhesive on a disposable surface, such as a sheet of weighing
paper.
c. Add a thin layer of adhesive to the back side of the mounting bracket.
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Leak Sensor Installation
d. Adjust the position of the mounting bracket as shown in Figure 42.
Figure 42. Appropriate position for the mounting bracket
Mounting
bracket
e. Gently press the mounting bracket against the drip tray for approximately two
minutes.
f.
Remove the locating fixture before the adhesive solidifies.
3. Pull the sinker frits above the solvent level in the solvent reservoir bottles. Then
disconnect the solvent lines from the degasser inlet ports and drain the solvent in the
solvent lines into a waste container.
4. Remove the LDA from the pump (see “Removing the LDA from the Pump” on page 75).
CAUTION Because the pump contains a high voltage supply, turn off the power to the
pump and unplug it from line power before you pull the chassis out of the enclosure.
CAUTION To avoid damage to the Interface board and the Controller board, observe
precautions for handling electrostatic sensitive devices.
5. Remove the pump chassis from the enclosure (see “Removing the Pump Chassis from the
Enclosure” on page 35).
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Leak Sensor Installation
6. Remove the cable that is connected to the J14 connector on the Interface board and the
P18 connector on the Controller board (see Figure 43).
In step 7 on page 52, you replace this cable with a cable that connects to the J14, P18,
and P8 connectors.
Figure 43. Pump chassis with a view of the Interface board and the Controller board
J14 connector
Thermo Scientific
P8 connector
P18 connector
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Leak Sensor Installation
7. Connect the new cable (see Figure 44) to the J14 connector on the Interface board and
the P18 and P8 connectors on the Controller board.
Figure 44. New cable (provided in the Leak Sensor Kit)
J14 connector
P18 connector
Leak sensor
connector
P8 connector
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Leak Sensor Installation
8. Slide the leak sensor onto the mounting bracket (see Figure 45).
Figure 45. Sliding the leak sensor onto the mounting bracket
Cable
Latch
Mounting bracket
9. Press the leak sensor latch against the mounting bracket until it clicks in place
(see Figure 46).
Figure 46. Leak sensor secured to the mounting bracket
Leak sensor
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Leak Sensor Installation
10. Route the leak sensor cable through the slot for the proportioning valve cables on the
front panel of the pump (see Figure 47).
Figure 47. Front panel of the pump with the LDA removed
Slot for the proportioning valve cables
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Leak Sensor Installation
11. Connect the leak sensor cable to the new P18 to J14 cable as shown in Figure 48.
Figure 48. Connection between the leak sensor cable and the new P18 to J14 cable
Leak sensor cable
New P18 to J14 cable
Connection between the
leak sensor cable and the
P18 to J14 cable
12. Reconnect the pump chassis to the enclosure (see “Reconnecting the Pump Chassis to the
Enclosure” on page 42).
13. Reattach the LDA to the pump’s front panel (see “Reattaching the LDA to the Pump” on
page 87).
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Leak Sensor Installation
14. Power on the pump.
While the drip tray is dry, the leak sensor’s LED remains green (see Figure 49). If solvent
comes in contact with the sensor, the leak sensor’s LED turns red (see Figure 50).
Figure 49. Green LED indicates a dry drip tray
Green LED
Figure 50. Red LED indicates a wet drip tray
Red LED
For information on specifying the configuration settings for the leak sensor, see Chapter 4,
“Instrument Driver Configuration.”
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Setting Up Continuous Flushing
Setting Up Continuous Flushing
If your Accela or Transcend pump is equipped with a seal wash pump, you can set up
continuous flushing of the piston guide bushings. Thermo Fisher Scientific recommends this
setup if you use mobile phases with a high concentration of salts.
The Seal Wash Pump Kit (see “Seal Wash Pump Kit” on page 128) contains the seal wash
pump, the tubing and fittings that you must have to set up the seal wash pump, and the cable
that connects the seal wash pump to the Controller board.
 To set up continuous flushing
1. Connect a reservoir of distilled water to the In port (see Figure 51) of the seal wash pump.
2. Using the tubing with the red, flangeless fitting that is provided in the Seal Wash Pump
Kit, connect the lower guide bushing to the Out port of the seal wash pump.
3. Using the tubing (without fittings) provided in the Seal Wash Pump Kit, connect the
lower wash tubing of the upper guide bushing to a waste reservoir.
Figure 51. Seal wash pump solvent line connections
Seal wash pump Seal wash pump
outlet
inlet
Thermo Scientific
Connection to
waste
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Instrument Driver Configuration
You can control one or two Accela pumps from your Thermo Scientific chromatography data
system or mass spectrometry application.
To configure the device driver for the Accela 600 Pump or Accela 1250 Pump, the Accela
pump must be turned on and connected to the data system computer.
To add instrument control for the Accela pump to the data system configuration, follow the
appropriate procedure in this chapter.
Contents
• ChromQuest Instrument Configuration
• Xcalibur Instrument Configuration
• Thermo Foundation Instrument Configuration
ChromQuest Instrument Configuration
To control the Accela 600 Pump or the Accela 1250 Pump from the ChromQuest data
system, add an Accela instrument to the ChromQuest Enterprise or add the pump to an
existing Accela instrument.
An instrument can include one or two Accela pumps.
 To add a new Accela instrument to the ChromQuest Enterprise
1. Open the ChromQuest data system.
The Main Menu window appears (see Figure 52).
IMPORTANT If the Instrument window of the ChromQuest data system is open,
close it. You can access the Main Menu window from the Instrument window and
modify the instrument’s configuration while the Instrument window is open, but you
must exit the Instrument window for the changes to take effect.
2. Right-click The Enterprise, and then choose New > Instrument from the shortcut menu
(see Figure 52).
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ChromQuest Instrument Configuration
Figure 52. Adding an instrument to the Enterprise
Action performed by choosing
this menu item
The New Instrument icon appears in the right pane.
3. To open the Instrument Configuration dialog box, do one of the following:
• Select the new instrument, and then choose File > Configure > Instrument.
–or–
• Right-click the new instrument and choose Configure > Instrument from the
shortcut menu.
The Instrument Configuration dialog box appears (see Figure 53).
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ChromQuest Instrument Configuration
Figure 53. Instrument Configuration dialog box
4. In the Instrument Name box, type a name for the instrument to distinguish it from other
instruments with different configurations or stack IDs.
5. In the Instrument Type list, select Accela.
The message shown in Figure 54 appears.
Figure 54. Message that appears when you select an instrument type
6. Do one of the following:
• To create a new instrument, click OK to close the message box.
–or–
• To modify the configuration of an existing instrument, click Cancel and do not make
a selection in the Instrument Type list.
7. Click Configure.
The Accela dialog box appears (see Figure 55).
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ChromQuest Instrument Configuration
Figure 55. Accela dialog box
To add one or two pumps to the instrument configuration, leave the Accela dialog box open
and go to the next procedure.
 To add one or two Accela pumps to the instrument configuration
1. Open the Accela dialog box as described in the previous procedure.
2. In the Available Modules list, double-click
(Accela Pump).
A copy of the Accela Pump icon appears in the Configured Modules pane.
3. In the Configured Modules pane, double-click
(Accela Pump).
The Accela Pump Configuration dialog box appears.
4. In the Serial Number list, select the serial number for the pump.
If you have connected two pumps to the data system computer, the last five digits of the
serial numbers for both pumps appear in the two Serial Number lists.
The serial numbers in the Serial Number list match the last five digits of the serial
numbers on the back panels of the pumps (see Figure 56).
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ChromQuest Instrument Configuration
Figure 56. Serial number on the pump’s back panel
355 River Oaks Parkway
San Jose, CA, U.S.A
Model: 1250 Pump
Serial No: 925000
The Status readback changes from Disconnected to Connected, and the Pump Type
readback for Pump 1 (and Pump 2 for a dual-pump system) specifies Accela 600 or
Accela 1250 (see Figure 57). In a dual-pump system, the two pumps can be different
pump types.
Figure 57. Accela Pump Configuration dialog box with the pump’s serial number selected
5. If your pump has a leak sensor, specify the leak sensor settings as follows:
a. Select the Use Leak Sensor check box (see Figure 57).
The Configure button becomes enabled.
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Xcalibur Instrument Configuration
b. Click Configure.
The Leak Sensor Configuration dialog box appears (see Figure 58).
Figure 58. Leak Sensor Configuration dialog box
c. Select the action that you want the pump to take if the leak sensor detects a leak.
d. Click OK to accept the setting and close the Leak Sensor Configuration dialog box.
6. In the Default Pressure Units list, select the pressure units that you want the data system
to display.
The available selections are PSI, bar, and MPa.
7. Click OK to accept the configuration and close the Accela Pump Configuration
dialog box.
8. Click OK to close the Accela dialog box.
9. Click OK to close the Instrument Configuration dialog box.
Xcalibur Instrument Configuration
If you are controlling the Accela 600 Pump or Accela 1250 Pump from a 2.0.7 version of the
Xcalibur data system, use the Xcalibur Instrument Configuration application to add the
pump to the instrument configuration.
 To add one or two Accela pumps to the Xcalibur instrument configuration
1. If the Xcalibur data system is open, close it.
1. Connect the pump or pumps to the data system computer.
2. Turn on pump 1 by pressing its power switch. If you have a dual-pump system, turn off
pump 2.
3. For Xcalibur versions below 2.1, from the computer desktop, choose Start > Programs >
Xcalibur > Instrument Configuration.
The Instrument Configuration window appears (see Figure 59).
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Xcalibur Instrument Configuration
Figure 59. Instrument Configuration window (for Xcalibur version 2.0.7)
4. To complete the configuration setup, do one of the following:
• To add one pump to the instrument configuration, go to step 5 on page 67 in
“Adding Pump 1 to the Instrument Configuration.”
• To add two pumps to the instrument configuration, go to step 5 of this procedure.
5. To add two pumps to the instrument configuration, do the following:
a. Follow step 5 through step 10 on pages 67 to 68 in “Adding Pump 2 to the
Instrument Configuration.”
b. Ensure that pump 2 is connected to the data system computer.
c. Turn on pump 2.
d. Reopen the Instrument Configuration window as described in step 3 on page 64.
e. Go to step 4 in “Adding Pump 2 to the Instrument Configuration” on page 69.
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Thermo Foundation Instrument Configuration
Thermo Foundation Instrument Configuration
You can control one or two Accela pumps from the data system computer. A dual-pump
system can contain two Accela 600 Pumps, two Accela 1250 Pump, or an Accela 600 Pump
and an Accela 1250 Pump. The Accela 600 Pump and Accela 1250 Pump drivers can
recognize and control either pump.
Tip To increase throughput for a gradient analysis, you can set up your liquid
chromatography/mass spectrometry (LC/MS) system in a dual-pump, dual-column
configuration and equilibrate one column while performing sample elution with the other
column.
To alternate columns between consecutive injections, use two instrument methods. In the
first method, pump 1 produces a gradient for sample elution on the first column, and
pump 2 produces a gradient that re-equilibrates the second column. In the second
method, pump 1 produces a gradient for sample elution on the second column, and
pump 2 produces a gradient that re-equilibrates the first column.
Using these alternating instrument methods (along with valve switching) allows for each
injection to run through a separate column and removes the wait time for column
equilibration.
To minimize the gradient delay volume between the eluting pump’s proportioning valve
and the autosampler’s injection valve, place the eluting pump on top of the equilibration
pump. Typically, the eluting pump is designated as pump 1 and the equilibration pump is
designated as pump 2.
Depending on whether you are adding one or two pumps to your instrument configuration,
follow one or both of these procedures:
• Adding Pump 1 to the Instrument Configuration
• Adding Pump 2 to the Instrument Configuration
Adding Pump 1 to the Instrument Configuration
 To add the pump (or pump 1 of a dual-pump system) to the instrument configuration
1. Connect the pump or pumps to the data system computer.
2. Turn on pump 1 by pressing its power switch. If you have a dual-pump system, turn off
pump 2.
3. Close the data system if it is open.
4. From the computer desktop, choose Start > Programs > Thermo Foundation 1.0 >
Instrument Configuration.
The Unloading Device Drivers status box appears (see Figure 60), followed by the
Thermo Foundation Instrument Configuration dialog box (see Figure 61).
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Thermo Foundation Instrument Configuration
Figure 60. Unloading Device Drivers status box
Figure 61. Thermo Foundation Instrument Configuration dialog box
5. In the Available Devices area, double-click the Accela 600 icon or the Accela 1250 icon.
Note Because you can install only one Accela pump driver, the Available Devices area
contains either an Accela 600 or Accela 1250 icon.
A copy of the Accela pump type icon appears in the Configured Devices area.
Tip Even though you can only install one pump driver, you can control both the
Accela 600 Pump and the Accela 1250 Pump from one data system computer because
the Accela 600 Pump and Accela 1250 Pump drivers can recognize and control both
pump models.
6. In the Configured Devices area, double-click the Accela 600 or the Accela 1250 icon.
The Accela pump type dialog box appears (see Figure 62). The Serial Number list contains
the serial number of the Accela pump that is powered on and connected to the data
system computer.
7. Ensure that the Serial Number list displays the pump’s serial number and that the Status
readback displays Connected.
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Thermo Foundation Instrument Configuration
The serial number is listed on the pump’s back panel (see Figure 56 on page 63).
Note The firmware version compatible with the current version of LC Devices is
subject to change.
Figure 62. Accela pump type dialog box
8. If your Accela pump has a leak sensor, select the Leak Sensor Installed check box.
The leak sensor is an option for the Accela 600 Pump and the Accela 1250 Pump. The
leak sensor has an optical sensor that turns red when covered by liquid.
For instructions about installing the leak sensor, see “Leak Sensor Installation” on
page 49.
For information about setting up leak sensor actions, such as stopping a run, see
“Specifying the Leak Sensor Settings in the Xcalibur Data System” on page 70.
9. Click OK to accept the configuration settings and close the Accela pump type dialog box.
10. Click Done to close the Instrument Configuration application.
If you have a dual-pump system, continue to the next procedure.
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4 Instrument Driver Configuration
Thermo Foundation Instrument Configuration
Adding Pump 2 to the Instrument Configuration
 To add pump 2 to the instrument configuration for a dual-pump system
1. Ensure that pump 2 is connected to the data system computer.
2. Turn on pump 2.
3. From the computer desktop, choose Start > Programs > Thermo Foundation 1.0 >
Instrument Configuration.
The Unloading Device Drivers status box appears (see Figure 60), followed by the
Thermo Foundation Instrument Configuration application.
4. In the Configured Devices area, double-click the Accela pump type icon.
The Accela pump type dialog box appears.
5. Select the Pump 2 check box (see Figure 63).
Figure 63. Accela 1250 Pump dialog box with the settings for a dual-pump system
6. Ensure that the Serial Number list displays the pump’s serial number and that the Status
readback displays Connected.
The serial number is listed on the pump’s back panel (see Figure 56 on page 63).
7. If your Accela pump has a leak sensor, select the Leak Sensor Installed check box.
8. Click OK to accept the configuration settings and close the Accela pump type dialog box.
9. Click Done to close the Instrument Configuration application.
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Instrument Driver Configuration
Specifying the Leak Sensor Settings in the Xcalibur Data System
Specifying the Leak Sensor Settings in the Xcalibur Data System
The leak sensor is a hardware option that can detect the presence of liquid on the pump’s drip
tray. When liquid comes in contact with the leak sensor, the leak sensor’s optical LED turns
green and the leak sensor sends a signal to the data system.
 To set up the leak sensor actions
1. Check the configuration settings for your Accela pump (see “Thermo Foundation
Instrument Configuration” on page 66). Ensure that the Leak Sensor Installed check
box is selected.
2. Open the Xcalibur Instrument Setup window.
3. In the view bar, click the icon that represents your Accela pump.
The instrument setup view for your Accela pump appears.
4. From the menu bar, choose Accela 600 Pump or Accela 1250 Pump > Options.
The Options dialog box appears (see Figure 64).
Figure 64. Options dialog box
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4 Instrument Driver Configuration
Specifying the Leak Sensor Settings in the Xcalibur Data System
5. Select the action that you want the pump to take when the leak sensor detects a leak.
The default selection is Do nothing.
Selection
Meaning
Do Nothing
The pump pistons continue moving.
Write Event to Log File Only
The pump pistons continue moving, but the
following text appears in the log file: Leak detected.
Set Instrument to Error State
after Run
When the current run ends, the pump pistons stop
moving and the sequence pauses.
Set Instrument to Error State
Immediately
When the leak sensor detects the presence of liquid
on the pump’s drip tray, the pump pistons stop
moving and the sequence pauses.
Tip To restart the pump after the leak sensor detects a leak, do the following:
1. Stop the sequence and delete it from the run queue.
2. Close the data system.
3. Fix the leak and make sure that the pump’s drip tray is dry and that the leak
sensor LED is green.
4. Reopen the data system and check the status of the instrument’s modules.
5. Resubmit the sequence.
–or–
1. Fix the leak and make sure that the pump’s drip tray is dry and that the leak
sensor LED is green.
2. Open the Information view in the Xcalibur data system.
3. Right-click the directory listing for the pump and choose Turn Device On from
the shortcut menu.
4. Click OK to accept the selection and close the Options dialog box.
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5
Maintenance
To maintain the optimal performance of your Accela pump, follow the “Maintenance
Schedule” on page 74 and the procedures in this chapter.
CAUTION Read all safety precautions before performing any maintenance procedures on
the pump. See “Safety Information” on page xvi.
CAUTION The instrument contains voltage lines. Switch off the power and disconnect the
power cable prior to servicing the instrument. There is no need to open the pump
enclosure, however, as all user serviceable components are outside of the instrument.
Contents
• Maintenance Schedule
• Accessing the LDA Components
• Replacing the Primary Piston Seals
• Replacing the Secondary Piston Seals
• Cleaning the Check Valves
• Cleaning the Pistons
• Cleaning the Instrument Casing
• Flushing the LDA
• Flushing the Piston Guide Bushings
• Removing Air from the Piston Chambers
• Cleaning the Displacement Chambers and the Inlet Module
• Replacing the Fuses
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Maintenance
Maintenance Schedule
Maintenance Schedule
To ensure optimal performance of the pump, perform preventive maintenance at regularly
scheduled intervals. Perform corrective maintenance when you observe poor performance or
when you have been instructed to do so by a Thermo Fisher Scientific field service
representative.
Tip With time, acetonitrile mobile phases coat the sapphire check valve seats with an
aliphatic amine residue that can cause the check valves to stick. To prevent residue
buildup, periodically pump HPLC-grade methanol or isopropanol (IPA) through the
system as described in “Flushing the LDA” on page 99. If you are using a buffered mobile
phase, pump a non-buffered solvent that is miscible with the buffered mobile phase
through the system before pumping methanol or IPA through the system.
Table 12 lists the recommended maintenance schedule for the pump.
Table 12. Maintenance frequency
74
Procedure
Frequency
Flushing the LDA
If the LC pump does not have the
99
optional seal wash pump, flush the LDA
once per day if you use buffered mobile
phases and once per month if you use
mobile phases that contain acetonitrile.
Flushing the Piston Guide Bushings
Once per day if you use buffered mobile 100
phases and you do not have the seal
wash pump installed
Replacing the Primary Piston Seals
Every 6 to 12 months depending on
usage
Cleaning the Check Valves
Every 6 to 12 months if you use buffered 98
mobile phases
Cleaning the Pistons
Every 6 to 12 months depending on
usage
98
Cleaning the Instrument Casing
As needed
99
Removing Air from the Piston
Chambers
As needed
101
Cleaning the Displacement Chambers
and the Inlet Module
As needed
102
Replacing the Fuses
As needed
103
Replacing the Secondary Piston Seals
As needed
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5 Maintenance
Accessing the LDA Components
Accessing the LDA Components
To replace the primary piston seals or to clean the components of the LDA, you must detach
the LDA from the pump, and then disassemble the LDA. To access the LDA components and
return the pump to operating condition, follow these procedures:
1. Removing the LDA from the Pump
2. Dismantling the LDA
3. Reassembling the LDA
4. Reattaching the LDA to the Pump
For a description of the LDA, see “Liquid Displacement Assembly (LDA)” on page 4.
Removing the LDA from the Pump
Remove the LDA to access the piston seals, which you replace during regularly scheduled
preventive maintenance.
Removing the LDA requires these tools:
Tool
Use
1/4 in. open-end wrench
To disconnect the high-pressure lines
#2 Phillips head screwdriver
To disconnect the chassis from the enclosure
To disconnect the LED cover
4 mm hex wrench
To loosen the clamping screws
To loosen the compression lid set screw
4 mm ball driver
To loosen the LDA attachment screws
To loosen the fastening screws that secure the holding
hooks to the LDA
2.5 mm ball driver
To remove the Z-arm cover
 To remove the liquid displacement assembly
1. Turn off the power to the pump.
2. Unplug all four inlet valves (see Figure 65).
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Maintenance
Accessing the LDA Components
Figure 65. Inlet valves connected to the LDA
Inlet valves
3. Disconnect all four solvent lines from the inlet module (see Figure 66 and Figure 67).
Figure 66. Pump with inlet valves cables removed and solvent lines connected
Solvent lines
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Accessing the LDA Components
Figure 67. Pump with the solvent lines disconnected
4. Using a 1/4 in. open-end wrench, unscrew the static mixer at the outlet of the LDA and
at the inlet of the outlet module (see Figure 68).
Figure 68. Static mixer connections to the LDA and the outlet module
LDA outlet
Thermo Scientific
Static mixer
Outlet module inlet
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Maintenance
Accessing the LDA Components
5. Using a 2.5 mm ball driver, loosen the two M3 × 4.0 cm screws on the Z-arm cover and
remove the cover (see Figure 69).
Figure 69. Z-arm cover in place over the Z-arms
Z-arm cover
M3 × 4.0 cm screws
6. Using a 4 mm hex wrench, loosen the two clamping screws by three or four turns
(see Figure 70).
Figure 70. Two clamping screws that secure the position of the pistons
Clamping screws
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5 Maintenance
Accessing the LDA Components
7. Pull out the two piston retaining springs (see Figure 71).
Figure 71. Location of the two piston retaining springs
Piston retaining springs
8. Gently push the piston heads to the left so that they do not contact the Z-arms
(see Figure 72).
Figure 72. Piston heads pushed to the left away from the Z-arms
Piston head
Thermo Scientific
Z-arm
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Maintenance
Accessing the LDA Components
9. Using a 4 mm ball driver, loosen the three LDA attachment screws (see Figure 73).
CAUTION To avoid breaking inlet valve D, do not touch or apply force to the valve
when loosening the LDA attachment screw.
Figure 73. Location of the LDA attachment screws
LDA attachment screws
Inlet valve D
10. Gently pull the LDA off the two guide pins. Do not let the pistons touch the Z-arms
(see Figure 74).
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5 Maintenance
Accessing the LDA Components
Figure 74. Removing the LDA from the pump
Piston
Guide pins
Z-arm
Dismantling the LDA
Dismantle the LDA as part of the procedure to replace the piston seals or if your Thermo
Fisher Scientific field service representative instructed you to do so as part of a corrective or
troubleshooting procedure.
 To disassemble the LDA
1. Remove the piston back flush tubes by gently pulling them off (see Figure 75).
Figure 75. Location of the piston back flush tubes
Piston back flush tubes
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Maintenance
Accessing the LDA Components
2. Put the LDA onto a level surface. Using a 4 mm ball driver, loosen, but do not remove,
the fastening screws that secure the holding hooks (see Figure 76).
Figure 76. Location of the fastening screws that secure the holding hooks
Fastening
screws
3. Carefully remove the pistons, guide bushings, and holding hooks from the LDA body
(see Figure 77).
Figure 77. LDA with the pistons, guide bushings, and holding hooks removed
Holding hook
Piston
Guide bushings
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5 Maintenance
Accessing the LDA Components
4. Using the 4 mm hex wrench, loosen the compression lid set screw. Remove the
compression lid by sliding it out (see Figure 78).
Figure 78. LDA with and without the compression lid
Compression lid
5. Slide out the outlet module, check valves, and displacement chambers (see Figure 79).
Figure 79. Sliding out the check valves, displacement chambers, and outlet modules
Check
valve
Check
valve
Lower
displacement
chamber
Thermo Scientific
Outlet
module
Upper
displacement
chamber
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Maintenance
Accessing the LDA Components
Reassembling the LDA
Reassemble the LDA after replacing the piston seals or completing a corrective or
troubleshooting procedure that requires you to disassemble it.
 To reassemble the LDA
1. Place the LDA on a clean, level surface.
2. Verify that the first check valve is facing the appropriate direction (see Figure 80), and
then insert it into the main body of the LDA (see Figure 81).
Figure 80. Check valve orientation
Figure 81. Inserting the displacement chambers, check valves, and outlet module into the LDA
Check
valve
Check
valve
Lower
displacement
chamber
Outlet
module
Upper
displacement
chamber
3. Insert the lower displacement chamber into the main body of the LDA.
4. Verify that the second check valve is facing the appropriate direction (see Figure 80), and
then insert it into the main body of the LDA.
5. Insert the upper displacement chamber into the main body of the LDA.
6. Insert the outlet module into the main body of the LDA.
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5 Maintenance
Accessing the LDA Components
7. Carefully slide the compression lid into the LDA main body (see Figure 82). If the
compression lid does not fit, verify that the parts are installed correctly.
Figure 82. Installing the compression lid
Compression lid
8. Using the 4 mm hex wrench, firmly tighten the compression lid screw (see Figure 83).
Figure 83. Tightening the compression lid screw
9. If you have not already done so, insert the pistons into the holding hook bores and
bushing guides. Align the piston wash tubes so that they protrude away from the holding
hook (see Figure 103 on page 97).
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Maintenance
Accessing the LDA Components
10. Carefully insert the piston into the bore of the displacement chamber and seal
(see Figure 84). At the same time, tighten the holding hook using the 4 mm ball driver.
Repeat the same procedure for the second piston unit.
Figure 84. Inserting the piston/guide bushing/holding hook assembly
11. Firmly tighten both holding hooks (see Figure 85).
Figure 85. Tightening the holding hooks
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5 Maintenance
Accessing the LDA Components
Reattaching the LDA to the Pump
Reattach the LDA to the pump after you have completed the routine or corrective
maintenance procedure that required you to remove it.
 To reattach the LDA to the pump
1. Align the pistons in such a way that they do not touch the Z-arms while you insert them.
Do not insert the piston springs at this time.
2. Reinstall the piston back flush tubes (see Figure 86).
Figure 86. LDA with the piston back flush tubes reinstalled
Piston back flush tubes
3. Place the LDA onto the drive unit using the two alignment pins as a guide
(see Figure 87).
Figure 87. Replacing the LDA onto the pump
Alignment pins
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Maintenance
Accessing the LDA Components
4. Using a 4 mm hex wrench, tighten the three LDA attachment screws (see Figure 88).
CAUTION When tightening the screw near the inlet valve, do not touch or apply
force on inlet valve D or it might break.
Figure 88. Tightening the LDA attachment screw near the inlet valve
Inlet valve D
LDA attachment screws
5. Insert the piston springs. The lip of the spring faces toward the right (see Figure 89).
Figure 89. Proper orientation of the piston springs
Piston retaining springs
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5 Maintenance
Accessing the LDA Components
6. Using the 4 mm wrench, tighten the two clamping screws (see Figure 90).
Figure 90. Tightening the two clamping screws
7. Replace the Z-arm cover and tighten the screws using a 2.5 mm ball driver
(see Figure 91).
Figure 91. Z-arm cover in place over the Z-arms
M3 × 4.0 mm screws
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Maintenance
Accessing the LDA Components
8. Carefully reconnect the static mixer (see Figure 92).
Figure 92. Static mixer connected to the LDA and the outlet module
Static mixer
9. Reconnect the solvent lines (see Figure 93).
Figure 93. Solvent lines connected to the LDA
Solvent lines
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5 Maintenance
Accessing the LDA Components
10. Connect the inlet valves (see Figure 94).
Figure 94. Inlet valves connected to the LDA
Inlet valves
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5
Maintenance
Replacing the Primary Piston Seals
Replacing the Primary Piston Seals
Replace the two primary piston seals with new piston seals as part of a regularly scheduled,
preventive maintenance routine or if you detect a leak from the LDA. Thermo Fisher
Scientific recommends replacing the primary piston seals every 6 to 12 months, depending on
your pump usage.
The primary piston seals for the Accela 600 Pump and the Accela 1250 Pump are made of
GFP and have a spring made of Hastelloy™, an alloy of stainless steel.
Figure 95. Primary piston seal with a view of the spring
spring
 To replace the primary piston seals
1. Remove the LDA from the pump (see “Removing the LDA from the Pump” on page 75).
2. Dismantle the LDA (see “Dismantling the LDA” on page 81).
3. Remove the piston from the holding hook and guide bushing (see Figure 96).
Figure 96. Piston, holding hook, and guide bushings assembly
Piston
Guide bushings
Holding hook
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5 Maintenance
Replacing the Primary Piston Seals
4. Insert the piston into the primary seal no more than 3 mm (0.12 in.) (see Figure 97). The
primary seal is located inside the displacement chamber.
Figure 97. Displacement chamber with piston inserted
Displacement chamber
Piston
5. Gently remove the seal by pulling on the piston while applying side force. Do not allow
the piston to touch any metal parts.
6. Remove the primary piston seal from the piston and discard the primary piston seal.
7. Insert the piston into the guide bushing.
8. With the spring (see Figure 95 on page 92) facing away from the guide bushing, slide the
replacement seal over the piston. Ensure that the replacement seal is flush with the guide
bushing and the piston protrudes out of the seal by 6 mm (0.24 in.) (see Figure 98).
Figure 98. Piston, guide bushing, and replacement piston seal assembly
Guide bushing
Piston seal
6 mm (0.24 in.)
9. Insert the piston into the bore of the displacement chamber. Do not apply any side load
onto the seal during this process (see Figure 99).
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Maintenance
Replacing the Primary Piston Seals
Figure 99. Using the guide bushing and piston to insert the piston seal
10. Hold down the guide bushing and slide the piston up until it is out of the seal
(see Figure 100).
Figure 100. Sliding the piston out of the seal
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5 Maintenance
Replacing the Primary Piston Seals
11. Remove the guide bushing/piston assembly from the displacement chamber
(see Figure 101).
Figure 101. Removing the bushing/piston assembly from the displacement chamber
12. Repeat step 3 through step 11 to replace the primary piston seal of the second
displacement chamber with a new piston seal.
13. Continue with one of the following:
• Perform other procedures that require access to the LDA (see “Removing the LDA
from the Pump” on page 75).
• Reassemble the LDA and reattach it to the pump (see “Reassembling the LDA” on
page 84 and “Reattaching the LDA to the Pump” on page 87).
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5
Maintenance
Replacing the Secondary Piston Seals
Replacing the Secondary Piston Seals
The secondary piston seals rarely require replacement. Replace the two secondary piston seals
if a Thermo Fisher Scientific representative has instructed you to do so as part of a corrective
or troubleshooting procedure.
The secondary piston seals are made of polyethylene (PE) for the Accela 600 Pump and GFP
for the Accela 1250 Pump.
 To replace the secondary piston seals
1. Remove the LDA from the pump (see “Removing the LDA from the Pump” on page 75).
2. Dismantle the LDA (see “Dismantling the LDA” on page 81).
3. Carefully guide the piston through the bore of the holding hook (see Figure 102).
4. Carefully slide a new secondary piston seal over the piston with the spring facing away
from the holding hook.
Figure 102. Holding hook with piston and seal
Piston seal (for the Accela 600 Pump)
5. Remove the secondary piston spring from the piston guide bushing.
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5 Maintenance
Replacing the Secondary Piston Seals
6. Carefully slide the piston guide bushing over the piston and align the piston wash tubes
so that they face away from the holding hook (see Figure 103).
Figure 103. Proper alignment of the piston wash tubes
Piston wash
tubes
7. Gently push the new secondary piston seal into its groove by pushing the guide bushing
up toward the end of the holding hook (see Figure 104).
Figure 104. Holding hook/piston/guide bushing assembly
8. Repeat step 3 through step 7 for the remaining secondary piston seal.
9. Continue with one of the following:
• Perform other procedures that require access to the LDA (see “Removing the LDA
from the Pump” on page 75).
• Reassemble the LDA and reattach it to the pump (see “Reassembling the LDA” on
page 84 and “Reattaching the LDA to the Pump” on page 87).
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5
Maintenance
Cleaning the Check Valves
Cleaning the Check Valves
If you use buffers with high concentrations of salts, clean the check valves as part of a regularly
scheduled, preventive maintenance procedure every 6 to 12 months depending on your usage.
Clean the check valves if they are not functioning properly or if you experience residual
pulsation that is too high.
CAUTION Do not use an ultrasonic bath to clean the check valves. Sonicating the check
valves can damage their small ruby balls.
 To clean the check valves
1. Remove the LDA from the pump body (see “Removing the LDA from the Pump” on
page 75).
2. Dismantle the LDA (see “Dismantling the LDA” on page 81).
3. Using a 10 mL syringe with a very short piece of silicon tubing at the Luer exit, gently
flush the check valves with 10 mL of distilled water.
4. Repeat step 3 with 10 mL of isopropanol.
5. Verify that fluid flows freely through the check valve. If you cannot achieve free flow
through the check valve, replace it with a new one.
6. Continue with one of the following:
• Perform other procedures that require access to the LDA (see “Removing the LDA
from the Pump” on page 75).
• Reassemble the LDA and reattach it to the pump (see “Reassembling the LDA” on
page 84 and “Reattaching the LDA to the Pump” on page 87).
Cleaning the Pistons
Clean the pistons as part of a regularly scheduled preventive maintenance procedure or if you
observe precipitate on them.
 To clean the pistons
1. Remove the LDA from the pump (see “Removing the LDA from the Pump” on page 75).
2. Dismantle the LDA (see “Dismantling the LDA” on page 81).
3. Moisten a soft tissue with isopropanol and wipe the pistons with it.
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Cleaning the Instrument Casing
4. Continue with one of the following:
• Perform other procedures that require access to the LDA (see “Removing the LDA
from the Pump” on page 75).
• Reassemble the LDA and reattach it to the pump (see “Reassembling the LDA” on
page 84 and “Reattaching the LDA to the Pump” on page 87).
Cleaning the Instrument Casing
If the pump enclosure becomes dirty, clean it with a soft cloth lightly moistened with a mild
detergent solution. Do not use any type of abrasive pad, scouring powder, or solvent.
Flushing the LDA
To maintain optimal performance of the LDA, do the following:
• If you use buffered mobile phases, flush the pump’s LDA daily with a non-buffered
mobile phase. Flushing the LDA helps prevent particulates from sticking in the check
valves, which might cause pressure problems.
• If you use mobile phases that contain acetonitrile, flush the LDA monthly with
HPLC-grade methanol or IPA. This removes the aliphatic amine residue that can build
up on the sapphire check valve seats and cause the check valves to stick.
 To flush buffered mobile phases, aliphatic amine residues, or both out of the LDA
1. If your chromatographic applications use buffered mobile phases, set up the system to
pump a non-buffered solvent that is miscible with the buffered mobile phase through the
LDA for 10 minutes.
2. If your chromatographic applications use acetonitrile mobile phases, pump HPLC-grade
methanol or IPA through the LDA for an additional 10 minutes.
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Maintenance
Flushing the Piston Guide Bushings
Flushing the Piston Guide Bushings
As the pistons move back and forth within the displacement chambers, a small quantity of
mobile phase leaks behind the primary piston seals. With buffered mobile phases, a precipitate
(also known as creep or salt build-up) forms as the liquid fraction evaporates. The abrasion
caused by this precipitate can scratch the piston and shorten the life of the piston seals.
If you use mobile phases that contain a high concentration of salts and you do not have the
seal wash pump, flush the piston seals at least once a day. The seal wash pump is an optional
pump that periodically flushes the piston guide bushings (see “Seal Wash Pump Installation”
on page 44).
 To flush the piston guide bushings
1. Connect a 10 mL syringe filled with distilled water to the bottom-outer tubing
(see Figure 105).
Figure 105. Syringe connected to the guide bushings for flushing
2. Gently push the water through the guide bushings.
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5 Maintenance
Removing Air from the Piston Chambers
Removing Air from the Piston Chambers
Air trapped in the piston chambers causes an excessive variation in the backpressure.
Note All low-pressure HPLC pumps require eluents that have been properly degassed.
The pump has a built-in vacuum degasser. Plumbing the pump to bypass this degasser
introduces a potentially unacceptable level of air into the piston chambers.
 To purge air out of the piston chambers
1. Open the purge valve by turning its wingnut counterclockwise (see Figure 106).
2. To collect the solvent being pumped through the liquid displacement assembly (LDA),
insert the tip of a 10 mL disposable syringe into the tubing attached to the purge valve.
Figure 106. Purge valve with tubing attached
Purge valve wingnut
Tubing
(with fitting
for syringe)
3. Pump 100% methanol or 100% acetonitrile through the LDA at a flow rate of
200 μL/min for 10 to 15 minutes.
Note Purging the pump at a low flow rate with 100% organic solvent works much
better than purging the pump at a high flow rate with (partly) aqueous eluents.
Note Due to seal friction, the system backpressure does not drop to zero when you
pump solvent through the open purge valve. Depending on the flow rate and solvent
composition, typical backpressure values range from 5 to 15 bar.
4. Close the purge valve, remove the syringe, and return to the initial conditions.
5. Check the pressure variation to determine if removing the trapped air stabilized the
system pressure.
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Maintenance
Cleaning the Displacement Chambers and the Inlet Module
Cleaning the Displacement Chambers and the Inlet Module
If the primary piston seals have started to decompose and you observe black, fibrous particles
in the chamber, clean the displacement chambers.
 To clean the displacement chambers and the inlet module
1. Remove the liquid displacement assembly (LDA) from the pump (see “Removing the
LDA from the Pump” on page 75).
2. Dismantle the LDA (see “Dismantling the LDA” on page 81).
3. Pry the piston seals out of the bores of the displacement chambers:
a. Carefully insert a piston into the hole in the center of the seal without pushing the
piston further into the bore of the displacement chamber.
b. Gently pry the seal out of the bore.
4. Using a needle, graphite pencil, or equivalent tool, carefully remove the PEEK film seals.
Be careful not to scratch the displacement chambers as you remove the film seals, as this
might cause leaking.
Note Both ends of the lower displacement chamber, the lower end of the upper
displacement chamber, and the bottom of the outlet module contain a PEEK film seal
for a total of five film seals.
5. Place the displacement chambers and the inlet module in a container of water and place
the container in an ultrasonic bath for 10 minutes.
6. Place the displacement chamber and the inlet module in a container of acetone and place
the container in an ultrasonic bath for 10 minutes.
7. Install the PEEK film seals if they are not damaged; otherwise, replace them with
new ones.
8. Replace the primary and secondary piston seals (see “Replacing the Primary Piston Seals”
on page 92 and “Replacing the Secondary Piston Seals” on page 96).
9. Continue with one of the following:
• Perform other procedures that require access to the LDA (see “Removing the LDA
from the Pump” on page 75).
• Reassemble the LDA and reattach it to the pump (see “Reassembling the LDA” on
page 84 and “Reattaching the LDA to the Pump” on page 87).
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5 Maintenance
Replacing the Fuses
Replacing the Fuses
Replace the pump fuses if you suspect that the current fuses are not functioning properly.
 To replace the fuses
1. Unplug the pump from the power outlet.
CAUTION To avoid an electrical shock, turn off the power to the pump and unplug
the pump from line power before you remove the fuse holder.
2. Using a flat head screwdriver, push the fuse holder lock to the right and pull out the
fuse box.
Figure 107. Power entry module and fuse label
Line
Fuse
100V 115V 230V
T2.0A
T1.0A
50/60 Hz
220VA
Fuse holder
lock
Fuse
holder
3. Remove the fuses and replace with the appropriate fuses.
Line power
Fuse
100 Vac or 115 Vac
5 × 20 mm, 2.0 A, 250 V, TL, UL, CSA
230 Vac
5 × 20 mm, 1.0 A, 250 V, TL, UL, VDE
4. Plug the pump power cord into the power outlet.
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6
Troubleshooting
To troubleshoot the pump’s performance, use the diagnostic tests and troubleshooting guides
in this chapter.
Contents
• Diagnostic Tests
• Error Messages
• Troubleshooting Guides
Diagnostic Tests
Run these tests if you want to validate the pump’s performance or if you suspect the pump is
not functioning properly.
• Testing the Flow Rate
• Testing the Residual Pulsation
• Testing the Pump Proportioning Accuracy
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Troubleshooting
Diagnostic Tests
Testing the Flow Rate
Run this test to compare the actual flow rate with the flow rate you assigned to the pump.
 To measure the actual flow rate
1. Connect a 40 bar (~580 psi) restrictor cartridge to the LDA outlet. Align the restrictor so
that the flow is in the direction of the arrow marked on the restrictor.
Figure 108. LDA attached to a restrictor
LDA outlet
Restrictor cartridge
2. Fill a mobile phase reservoir with HPLC-grade methanol and connect it to inlet
channel A.
3. Use the instrument control system to set channel A to 100%.
4. Set the flow rate to 1 mL/min.
5. Do one of the following:
• Connect the other end of the restrictor cartridge to an instrument that measures
flow rate.
• Connect the other end of the restrictor cartridge to a length of tubing and place the
tubing into an empty, 10 mL graduated cylinder.
6. Start the pump, wait 2 minutes, and then stop the pump.
7. If you do not have a flow rate meter, measure the fluid in the graduated cylinder. The
actual flow rate equals the measured volume divided by two.
8. Compare the actual flow rate with the set flow rate. Under these conditions, the accuracy
of the flow should be ±0.5%.
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6
Troubleshooting
Diagnostic Tests
9. If your flow rate is unstable or inaccurate, see Table 13.
Table 13. Reasons for unstable flow rates
Possible reason for unstable flow rate
Correction
Damaged or dirty check valve
Clean the check valves. See “Cleaning the
Check Valves” on page 98.
Worn out primary piston seals
Replace the seals. See “Replacing the Primary
Piston Seals” on page 92.
Unstable load (column)
Use a backpressure restrictor or another
column.
Testing the Residual Pulsation
This test measures the pump pulsation.
 To measure the pump pulsation
1. Connect a 40 bar (~580 psi) restrictor cartridge to the LDA outlet. Align the restrictor so
that the flow is in the direction of the arrow marked on the restrictor (see Figure 109).
Figure 109. LDA attached to a restrictor
LDA outlet
Restrictor cartridge
2. Fill a mobile phase reservoir with HPLC-grade methanol and connect it to inlet
channel A.
3. Open the purge valve.
Tip For information on viewing the pump’s status and starting and stopping the
solvent flow from the pump, refer to the data system Help.
4. Set the pressure unit to bar.
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6
Troubleshooting
Diagnostic Tests
5. Verify that the pressure is 0.
6. Set channel A to 100%.
7. Set the flow rate to 500 μL/min.
8. Start the pump, wait 1 minute, and then close the purge valve.
9. Wait 5 minutes to allow the pump pressure to equilibrate.
10. Observe the pressure graph for pressure fluctuations. The pressure standard deviation
should be less than 1 bar (14.5 psi).
Testing the Pump Proportioning Accuracy
This procedure tests the pump’s proportioning accuracy.
 To test the pump proportioning accuracy
1. Set up the system as follows:
a. Plumb your system to bypass the autosampler.
b. Connect a 40 bar (~580 psi) restrictor cartridge to the LDA outlet.
c. Connect the other end of the cartridge to a UV detector with an analytical flowcell
(see Figure 110).
Figure 110. LDA attached to a flow restrictor
d. Fill a mobile phase reservoir bottle with HPLC-grade methanol and connect it to
inlet channels A and B.
e. Fill a mobile phase reservoir bottle with HPLC-grade methanol spiked with 0.5%
acetone and connect it to inlet channels C and D.
2. Draw solvent through all four channels:
a. Set each channel to 25%.
b. Open the purge valve.
c. Set the flow rate to 1000 μL/min and start the pump.
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6
Troubleshooting
Diagnostic Tests
d. Wait 2 minutes, and then close the purge valve.
e. Wait an additional 2 minutes.
3. Pump 100% methanol and stabilize the baseline at 254 nm as follows:
a. Set channel A to 100%.
b. Set the UV detector to monitor 254 nm.
c. Wait until the system is equilibrated (that is, until the baseline is stable).
d. Set the detector to zero at 254 nm.
e. Stop the pump.
4. Use Table 14 to create a method to run a step gradient two times. Set the detector to
acquire data at 254 nm for 160 minutes.
5. Run the method and acquire the data.
Table 14. Gradient file for quaternary pumps (Sheet 1 of 2)
Thermo Scientific
Time
%A
%B
%C
%D
Flow rate (μL/min)
0
100
0
0
0
1000
5
100
0
0
0
1000
10
0
0
0
100
1000
15
0
0
0
100
1000
15.1
20
0
0
80
1000
20
20
0
0
80
1000
20.1
40
0
0
60
1000
25
40
0
0
60
1000
25.1
60
0
0
40
1000
30
60
0
0
40
1000
30.1
80
0
0
20
1000
35
80
0
0
20
1000
35.1
100
0
0
0
1000
40
0
100
0
0
1000
45
0
0
100
0
1000
50
0
0
100
0
1000
50.1
0
20
80
0
1000
55
0
20
80
0
1000
55.1
0
40
60
0
1000
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6
Troubleshooting
Diagnostic Tests
Table 14. Gradient file for quaternary pumps (Sheet 2 of 2)
Time
%A
%B
%C
%D
Flow rate (μL/min)
60
0
40
60
0
1000
60.1
0
60
40
0
1000
65
0
60
40
0
1000
65.1
0
80
20
0
1000
70
0
80
20
0
1000
70.1
0
100
0
0
1000
75
0
100
0
0
1000
6. Determine the height (absorbance value) of the steps (A through J) from baseline to top
(see Figure 111).
Figure 111. Step gradient profile for testing compositional accuracy
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6
Troubleshooting
Diagnostic Tests
7. Perform the calculations in Table 15.
Table 15. Step gradient calculation
Step%
20%
Calculation
(E + J)/2
× 100 =
(A + F)/2
40%
(D + I)/2
× 100 =
(A + F)/2
60%
(C + H)/2
× 100 =
(A + F)/2
80%
(B + G)/2
× 100 =
(A + F)/2
8. Compare your results for each step gradient with the pass criteria in Table 16.
Table 16. Pass criteria for gradient test.
Gradient forming performance
Pass criteria
20% Step Height: _______%
19.5–20.5%
40% Step Height: _______%
39.5–40.5%
60% Step Height: _______%
59.5–60.5%
80% Step Height: _______%
79.5–80.5%
20% Step Height: _______%
19.5–20.5%
40% Step Height: _______%
39.5–41.5%
60% Step Height: _______%
59.5–60.5%
80% Step Height: _______%
79.5–80.5%
9. If any value falls outside the range, call Thermo Fisher Scientific Technical Support.
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6
Troubleshooting
Error Messages
Error Messages
Table 17 describes the pump-related error messages that might appear in the system control
software event log or status area.
Table 17. Error messages
112
Error message
Description
Low Pressure Error
The system pressure has fallen below the set minimum
pressure.
High Pressure Error
The system pressure has risen above the set maximum
pressure.
Index Error
The system expected an index signal that never arrived.
Physical problem with the hardware or connectors: Contact
an authorized dealer.
Leak detected
Indicates the presence of liquid on the pump’s drip tray.
Motor Error
The motor is malfunctioning. Indicates that too much torque
is required to drive the pump accurately.
Position Error
The system was unable to maintain accurate positioning of
the motor. Restart the pump.
Power Failure Error
The drive motor has drawn excessive current.
Solenoid Error
A proportioning valve’s solenoid has been disconnected or is
defective. Physical problem with the hardware or connectors:
Contact an authorized dealer.
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
6 Troubleshooting
Troubleshooting Guides
Troubleshooting Guides
This section provides troubleshooting guides for the pump and degasser.
Pump Troubleshooting Guide
Table 18 describes symptoms you might observe with the pump, possible causes for the
symptom, and corrective actions.
Table 18. Pump health check/troubleshooting guide
Symptom
Possible cause
Solution
No response when
power is switched on
Power cord not properly installed
Place cord correctly.
Defective power cord
Replace cord.
Blown fuse
Replace the fuse.
Communication cable not firmly installed
Reinstall the cable.
Defective communication cable
Replace the cable.
Wrong or corrupted firmware
Download the current firmware to the
pump.
Check valve failure
Clean/replace the check valve. See
“Cleaning the Check Valves” on
page 98.
Immiscible solvents
Test/change the solvent system.
Insufficient degassing
Use helium degassing.
Solvent frit clogged
Replace the solvent frit.
Insufficient system pressure
Add a backpressure regulator or increase
the flow rate.
Defective seals
Replace the primary piston seals. See
“Replacing the Primary Piston Seals” on
page 92.
Valve failure
Clean/replace the check valve. See
“Cleaning the Check Valves” on
page 98.
Pressure error
Check the pressure limits.
No connection to
software
Flow
unstable/pulsation
No flow from the
pump
Pump remote start/stop Incorrect wiring
not working
Incorrect software/gradient table settings
Thermo Scientific
Check the wiring.
Check the method and the method
options.
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6
Troubleshooting
Troubleshooting Guides
Degasser Troubleshooting Guide
Table 19 describes symptoms you might observe with the degasser, probable causes of the
symptoms, and corrective actions.
Table 19. Degasser health check/troubleshooting guide
Symptom
Probable cause
Yellow status LED is on steadily,
pump is running, and RPM seems
high.
Pump is in start up phase or system’s If vacuum pump speed continues to rise
degassing demand has increased.
for an extended period of time (as heard
by the pitch of the stepper motor), it
might indicate a potential fault
condition.
Yellow status LED is flashing
approximately 1 second off,
1 second on. Vacuum pump is not
running.
Possible leak.
Contact your Thermo Fisher Scientific
field service representative.
Yellow status LED is flashing
approximately 2 seconds off,
1 second on. Vacuum pump is not
running.
Possible sensor or control board
fault.
Contact your Thermo Fisher Scientific
field service representative.
Power and Vacuum LEDs
illuminate green, but the pump
cannot be heard.
Due to the design of the pump and
degasser, the pump is very silent at
low RPMs, even though vacuum is
good and degassing is normal.
Monitor the UV absorbance of
non-degassed methanol at 215 nm
versus degassed methanol coming
through the degasser. Proper
performance of the degasser should
decrease the UV absorbance of the
methanol significantly.
Bubbles move through the output
tubing.
Loose fitting or fittings.
Tighten the input and output fittings.
No solvent flow.
If a buffer was left in the degasser for Prime/purge the pump head.
some time after use, it might plug
Use a different channel, or connect the
the degasser.
channel to a beaker of the solvent
without the buffer. Draw the solvent
through the channel to dissolve the
buffer. Do not push the solvent through
the channel. If this flushing action does
not work, contact your Thermo Fisher
Scientific field service representative.
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Solution
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7
Replacement Parts
This chapter contains a list of replaceable parts and their corresponding part numbers. To
place an order, contact your local Thermo Fisher Scientific customer service representative or
technical support.
Contents
• Main Body
• Accela 600 Pump LDA
• Accela 1250 Pump LDA
• Inlet Valve Assembly
• Service Spares
• Accela 600 Pump Accessory Kit
• Accela 1250 Pump Accessory Kit
• Inlet Tubing Kit
• Compression Fittings for High-Pressure Connections
• Tools
• Seal Wash Pump Kit
• Dynamic Mixer
• Leak Sensor Kit
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Replacement Parts
Main Body
Main Body
The replaceable parts listed in Table 20 correspond to the items shown in Figure 112. These
parts are compatible with both the Accela 600 Pump and the Accela 1250 Pump.
Table 20. Main body assembly parts
116
Item
Description
Part number
1
Degassing bracket assembly, Accela
00950-01-00297
2
Screw, ISO 4762, M3 × 8a
N/A
3
Cover, metal plate, Accela 600
N/A
4
LDA, Accela 600
00950-01-00294
5
Mixer static, Accela 600
00950-01-00292
6
Placeholder Accela 600
00950-01-00293
7
Piston spring stainless steel
00201-11324
8
Z-arm cover Accela 600
N/A
b
9
Screw, M3 × 4.0
10
Front panel Accela 600
N/A
11
Drip tray Accela 600
60057-40001
12
Cable valve D, Accela 600
N/A
13
Inlet module Accela 600
N/A
14
Cable valve C, Accela 600
N/A
15
Cable valve B, Accela 600
N/A
16
Cable valve A, Accela 600
N/A
N/A
Cable assembly, proportioning valve, Accela 600
(includes the cable valves A, B, C, and D and other
cables)
00950-01-00289
a
The length of the screw is 8 cm and the diameter is 3 mm.
b
The length of the screw is 4 cm and the diameter is 3 mm.
Accela 600 and 1250 Pump Hardware Manual
N/A
Thermo Scientific
7
Replacement Parts
Main Body
Figure 112. Main pump—front view
(1)
Degassing
bracket
(2)
Screw ISO 4762
M3 × 8
(3)
Blind cover
(4)
LDA
(5)
Mixer
static
(6)
Placeholder blind outlet module
(7)
Piston spring
(8)
Z-arm cover
(9)
Screw
M3 × 40
(16)
Cable
valve A
Thermo Scientific
(15)
Cable
valve B
(14)
Cable
valve C
(13)
Inlet
Module
(12)
Cable
valve D
(11)
Drip tray
(10)
Front panel
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7
Replacement Parts
Accela 600 Pump LDA
Accela 600 Pump LDA
The replaceable parts listed in Table 21 correspond to the items shown in Figure 113.
The Accela 600 Pump has ceramic pistons. The primary piston seal is made of graphite
fiber-reinforced polytetrafluoroethylene (GFP) and the secondary piston seal is made of
polytetrafluoroethylene (PTFE).
Table 21. Accela 600 Pump LDA parts
118
Item
Quantity
Description
1
1
Outlet module, complete
00950-01-00284
(compatible with both the Accela 600 Pump
and the Accela 1250 Pump)
2
5
UHP PEEK sealing ring
00950-01-10013
3
1
Displacement chamber, upper
00950-01-00282
4
2
Check valve, titanium
00110-05110
5
1
Displacement chamber, lower
00950-01-00281
6
1
UHP LDA main body
N/A
7
2
Piston unit (S-finish), ceramic
00201-11324
8
2
Holding hook
N/A
9
2
Secondary piston seal, polyethylene (PE)
00107-18114
10
2
Guide bushing
00950-01-00283
11
2
Primary piston seal,
graphite fiber-reinforced
polytetrafluoroethylene (GFP)
00107-18110
12
1
Adapter, LDA
N/A
13
1
Compression lid including set screw
00950-01-00125
Accela 600 and 1250 Pump Hardware Manual
Part number
Thermo Scientific
7
Replacement Parts
Accela 600 Pump LDA
Figure 113. Accela 600 Pump LDA parts
(1)
Outlet module
(2)
UHP sealing ring, PEEK
(3)
Upper displacement chamber
(4)
Check valve, titanium
(5)
Lower displacement chamber
(6)
UHP LDA main body
(7)
Piston unit (S-finish), ceramic
(8)
Holding hook
(9)
Secondary piston seal (PE)
(13)
Compression lid
including set
screw
Thermo Scientific
(12)
LDA adapter
(11)
Primary piston
seal (GFP)
(10)
Guide bushing
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7
Replacement Parts
Accela 1250 Pump LDA
Accela 1250 Pump LDA
The replaceable parts listed in Table 22 correspond to the items shown in Figure 114.
The Accela 1250 Pump has sapphire pistons and graphite fiber-reinforced
polytetrafluoroethylene (GFP) primary and secondary piston seals.
Table 22. Accela 1250 Pump LDA parts
120
Item
Quantity
Description
1
1
Outlet module, complete
00950-01-00284
(compatible with both the Accela 600 Pump
and the Accela 1250 Pump)
2
5
UHP PEEK sealing ring
00950-01-10013
3
1
Displacement chamber, upper
00950-01-00123
4
2
Check valve, titanium and ruby
00110-05110
5
1
Displacement chamber, lower
00950-01-00121
6
1
UHP LDA main body
N/A
7
2
Piston unit, sapphire
00950-01-00126
8
2
Holding hook
N/A
9
2
Secondary piston seal, (GFP)
00950-01-00129
10
2
Guide bushing
00950-01-00283
11
2
Primary piston seal, (GFP)
00950-01-00129
12
1
Adapter, LDA
N/A
13
1
Compression lid including set screw
00950-01-00125
Accela 600 and 1250 Pump Hardware Manual
Part number
Thermo Scientific
7
Replacement Parts
Accela 1250 Pump LDA
Figure 114. Accela 1250 Pump LDA parts
(1)
Outlet module
(2)
UHP PEEK sealing ring
(3)
Upper displacement chamber
(4)
Check valve, titanium
(5)
Lower displacement chamber
(6)
UHP LDA main body
(7)
Sapphire piston
(8)
Holding hook
(9)
Secondary piston seal (GFP)
(13)
Compression lid
including set
screw
(11)
Primary piston
seal (GFP)
(10)
Guide bushing
(12)
LDA adapter
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Replacement Parts
Inlet Valve Assembly
Inlet Valve Assembly
The replaceable parts listed in Table 23 correspond to the items shown in Figure 115.
Table 23. Inlet valve assembly parts (compatible with both pumps)
Item
Quantity
Description
Part number
1
1
Flow brake (Accela 600)
00950-01-00287
2
1
O-ring, 2.69 × 1.14 mm
00950-01-00286
3
4
Parts for valve fixation
00950-01-00288
4
4
Valve (Accela 600)
00950-01-00285
Figure 115. Inlet valve assembly
(1)
Flow
brake
122
Accela 600 and 1250 Pump Hardware Manual
(2)
O-ring
2.69 × 1.14
(3)
Parts for valve
fixation
(4)
Valve
Thermo Scientific
7
Replacement Parts
Service Spares
Service Spares
Table 24 lists services spares common to the Accela 600 Pump and the Accela 1250 Pump
that you can order from Thermo Fisher Scientific.
Table 24. Service spares that are common to both pumps (Sheet 1 of 2)
Thermo Scientific
Description
Part number
Brake, flow, inlet module, Accela 600 Pump
00950-01-00287
Cable assembly, AC power, internal
60157-63014
Cable assembly, RS-232, internal, Degasser board
60157-63004
Cable assembly, fan, Diablo, UHPLC
60157-63002
Cable assembly, RS-232 internal, Interface board
60157-63012
Cable assembly, COMM and Gradient LED
60157-63010
Cable assembly, Degasser LED
60157-63008
Cable assembly, LED, front panel
60157-63006
Cable assembly, proportioning valve, Accela 600 Pump, RoHS
00950-01-00289
Cable assembly, force sensor, Accela 600 Pump, RoHS
00950-01-00290
Cable assembly, digital I/O, Accela 600 Pump, RoHS
00950-01-00291
Cable, Bowden, Accela
00302-99-00010
Cable, motor index, MS pump
00950-01-00001
Cable, USB 2.0, A to B, 12 in. UL RoHS
00302-99-00009
Fuseholder, drawer, 5 × 20 mm, 2 pole, safe, RoHS
2109-0740
Fuse, 1.0 A, 250 V, 5 × 20 mm, time-lag, UL, VDE, RoHS
N/A
Fuse, IEC, 2.0 A, 250 V, 5 × 20 mm, time-lag, UL, CSA, RoHS
N/A
Kit, Inlet Tubing, Accela 600 Pump
60157-62008
Kit, Proportioning Valve Fixation, Accela 600 Pump
00950-01-00288
Mixer, static, 65 μL, Accela 600 Pump, RoHS
00950-01-00292
Module, outlet, Accela 600 Pump
00950-01-00284
O-ring, 2.69 × 1.14 mm, perfluor
00950-01-00286
PCB, UHPLC Interface board
60157-61000
Placeholder for the dynamic mixer, blind outlet module,
Accela 600 Pump, RoHS
00950-01-00293
PCB, Control, new, Accela 600 Pump, RoHS
00950-01-00296
Power supply, 24 V, 2.5 A, 60 W, RoHS
00012-01-00013
Seal, face, PEEK, check valve
00950-01-10013
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7
Replacement Parts
Service Spares
Table 24. Service spares that are common to both pumps (Sheet 2 of 2)
Description
Part number
Spring, piston retaining
00201-11328
Valve, proportioning, Accela 600 Pump
00950-01-00285
Valve, check
00110-05110
Table 25 lists the services spares for the Accela 600 Pump that you can order from Thermo
Fisher Scientific.
Table 25. Accela 600 Pump service spares
Description
Part number
Bushing, guide, Accela 600 Pump
00950-01-00283
Chamber, displacement, lower, Accela 600 Pump
00950-01-00281
Chamber, displacement, upper, Accela 600 Pump
00950-01-00282
Gearbox, new, Accela 600 Pump, RoHS
00950-01-00295
Piston, 1/8 in. diameter, tetragonal zirconia polycrystal (TZP)
00201-11324
Seal, piston, secondary, PE
00107-18114
Seal, piston, primary, GFP
00107-18110
Table 26 lists the services spares for the Accela 1250 Pump that you can order from Thermo
Fisher Scientific.
Table 26. Accela 1250 Pump service spares
124
Description
Part number
Bushing, guide, Accela Pump
00950-01-00127
Chamber, displacement, lower, Accela 1250 Pump
00950-01-00121
Chamber, displacement, upper, Accela 1250 Pump
00950-01-00123
Gear box, Accela 1250 Pump
00950-01-00308
Piston, sapphire
00950-01-00126
Seal, piston, secondary and primary, GFP
00950-01-00129
Spring, piston retaining
00201-11328
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
7 Replacement Parts
Accela 600 Pump Accessory Kit
Accela 600 Pump Accessory Kit
Table 27 lists the parts provided in the Accela 600 Pump Accessory Kit.
Table 27. Accela 600 Pump Accessory Kit (P/N 60157-62006)
Quantity
Description
Part number
1
Luer adapter
N/A
2
Fuse, 1.0 A, 250 V, 5 × 20 mm, time-lag, UL, VDE,
RoHS
N/A
1
Fuse, IEC, 2.0 A, time-lag, 5 × 20 mm, RoHS, UL, CSA
N/A
1
Wrench, 1/4 × 5/16 in., super krome, RoHS
N/A
1
Tubing, convoluted, pump waste
F5034-040
1
Syringe, Luer-Lok™, 10 mL
N/A
1
Screwdriver, balldriver, hex, 4 mm, RoHS
N/A
1
Connector, cable, plug, 8 positions, 3.81 mm pitch,
Phoenix, RoHS
00004-02511
1
Cable, USB, A to B, 3.0 m, shielded, RoHS
00302-99-00014
1
Wrench, L-hex, 4 mm, RoHS
N/A
1
Tool, balldriver, 2.5 mm
N/A
2
Seal, high-pressure
00107-18110
1
Tool, fitting extender, Accela 600 Pump
00725-01-00023
1
Kit, Inlet Tubing, Accela 600 Pump
60157-62008
Accela 1250 Pump Accessory Kit
Table 28 lists the parts provided in the Accela 1250 Pump Accessory Kit (P/N 60157-62009).
Table 28. Accela 1250 Pump Accessory Kit (Sheet 1 of 2)
Thermo Scientific
Quantity
Description
Part number
1
Luer adapter
N/A
1
Fuse, IEC, 2.0 A, time-lag, 5×20 mm, RoHS, UL, CSA
N/A
2
Fuse, 1.0 A, 250 V, 5 × 20 mm, time-lag, UL, VDE,
RoHS
N/A
1
Wrench, 1/4 × 5/16 in., super krome, RoHS
N/A
1
Tubing, convoluted, pump waste
F5034-040
1
Syringe, Luer-Lok, 10 mL
N/A
Accela 600 and 1250 Pump Hardware Manual
125
7
Replacement Parts
Inlet Tubing Kit
Table 28. Accela 1250 Pump Accessory Kit (Sheet 2 of 2)
Quantity
Description
Part number
1
Screwdriver, balldriver, hex, 4 mm, RoHS
N/A
1
Connector, cable, plug, 8 positions, 3.81 mm pitch,
Phoenix, RoHS
N/A
1
Cable, USB, A to B, 3.0 m, shielded, RoHS
00302-99-00014
1
Wrench, L-hex, 4 mm, RoHS
N/A
1
Tool, balldriver, 2.5 mm
N/A
2
Seal, high-pressure, GFP, Accela Pump
00950-01-00129
1
Tool, fitting extender, Accela 600 Pump
00725-01-00023
1
Kit, Inlet Tubing, Accela 600 Pump
60157-62008
Inlet Tubing Kit
The Accela 600 Pump Inlet Tubing Kit contains four solvent reservoir lines. Each solvent line
consists of a 3 m length of 1/8 in. OD×1/16 in. ID, FEP tubing with a 1/4-28 PEEK
flangeless fitting at one end and a 20 μm particle size, stainless steel filter at the other end.
Table 29 lists the parts provided in the Accela 600 Pump Inlet Tubing Kit
(P/N 60157-62008).
Table 29. Accela 600 Pump Inlet Tubing Kit
a
126
Quantity
Description
Part number
288 in.
0.062 in. ID × 1/8 in. OD, FEP tubing (order by the 3219-2004
inch)
4
Filter, solvent inlet, stainless steel, 20 μm, RoHS
4
Fitting, ferrules for 1/8 in. OD tubing, short, Tefzel™ 00101-18223
1
Fitting, nut, for 1/8 in. OD tubing, 1/4-28a, PEEK,
blue, RoHS
N/A
1
Fitting, nut, for 1/8 in. OD tubing, 1/4-28, PEEK,
yellow, RoHS
N/A
1
Fitting, nut, for 1/8 in. OD tubing, 1/4-28, PEEK,
green, RoHS
N/A
1
Fitting, nut, for 1/8 in. OD tubing, 1/4-28, PEEK,
orange, RoHS
N/A
00109-02-00022
The nut has a 1/4 inch thread barrel with 28 threads per inch.
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
7 Replacement Parts
Compression Fittings for High-Pressure Connections
Compression Fittings for High-Pressure Connections
Table 30 lists the fittings used to connect the high-pressure solvent line between the pump
and the autosampler.
Table 30. Compression fitting for high-pressure connections and 1/16 in. OD tubing
Description
Part number
Compression nut, stainless steel, 0.45 in. length
2522-1880
Ferrule, stainless steel, 0.188 in. length
00101-18187
Tools
Table 31 lists the tools that you must have to perform maintenance on the pump.
Table 31. Tools required to maintain the pump
Thermo Scientific
Description
Part number
Tool, fitting extender, Accela 600 Pump
00725-01-00023
Balldriver, hex, 4 mm, RoHS
N/A
Wrench L hex 4 mm, RoHS
N/A
Ball driver, 2.5 mm
N/A
Wrench, 1/4 × 5/16 in., super krome, RoHS
N/A
Accela 600 and 1250 Pump Hardware Manual
127
7
Replacement Parts
Seal Wash Pump Kit
Seal Wash Pump Kit
The Seal Wash Pump Kit (P/N 00960-01-00023) contains the seal wash pump, the tubing
and fittings that you must have to set up the seal wash pump, and the cable that connects the
seal wash pump to the Controller board. Figure 116 through Figure 118 show the
components of the Seal Wash Pump Kit.
Figure 116. Tubing, fittings, solvent filter, and solvent bottle in the Seal Wash Pump Kit
Figure 117. Seal wash pump in the Seal Wash Pump Kit
Figure 118. Cable for seal wash pump
Connect to the
seal wash pump
128
Accela 600 and 1250 Pump Hardware Manual
Connect to the
Controller board
Thermo Scientific
7
Replacement Parts
Dynamic Mixer
Dynamic Mixer
The part number for the optional dynamic mixer (see Figure 119) is 00950-01-00298.
Tip To secure the dynamic mixer to the Accela 600 Pump, use the screws that secure the
blind outlet (placeholder) module.
Figure 119. Dynamic mixer
Outlet port
Connects to the P3 cable within the
pump chassis
Inlet port
Leak Sensor Kit
The Leak Sensor Kit (P/N 00960-01-00024) contains the following items:
• Leak sensor with attached cable
• Mounting bracket
• Locating fixture
• Two-component adhesive
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
129
I
Index
A
Accela Autosampler
high-pressure solvent line connection 30
interconnect cable 19
Accela Open Autosampler
adapter cable for detector 21
high-pressure solvent line connection 31
interconnect cable 20
acetonitrile mobile phases, using 74
air
removing from the degassing unit 27
removing from the piston chambers 101
B
back panel
clearance requirements 11
computer connections 17
D-sub 25-pin connector 23
interconnect cable connection 17
power cable connection 16
benchtop space requirements 11–14
buffered mobile phases, using 5
C
cables
interconnect (contact closure) 18
power cord 16
USB 17
check valves
cleaning 98
sticking 99
checklist, installation 10
cleaning
check valves 98
displacement chambers 102
inlet module 102
instrument casing 99
pistons 98
Thermo Scientific
compliance
FCC iv
regulatory iii
WEEE vii
computer, connecting 17
configuration
for the ChromQuest data system 59
for the Thermo Foundation application 66
for the Xcalibur 2.0.x data system 64
connections
interconnect cable 18
low-pressure solvent lines 24
USB cable 17
contact closure cables
Accela Autosampler 18
Accela Open Autosampler 20
continuous flushing 5, 57
D
degasser
connecting solvents 25
description 2, 4
priming membranes 25
troubleshooting guide 114
D-sub 25-pin connector 23
dual-pump system
contact closure, Accela Open Autosampler 20–22
Thermo Foundation Instrument Configuration 66
dynamic mixer
description 4
installation 34
ordering information 129
E
electromagnetic compatibility iv
eluting pump, contact closure connection with an Accela
Open Autosampler 21
EMC compliance iii–iv
environmental conditions xvii
error messages 112
Accela 600 and 1250 Pump Hardware Manual
131
Index: F
F
FCC compliance iv
film seals, removing 102
firmware
Accela 1250 Pump 8
Accela 600 Pump 7
fittings
flangeless for low-pressure solvent lines 25
two-piece compression 29
flow rate
range 2
testing 105
flushing, continuous 57
footprint, LC system 11
fuses, replacing 102
H
high-pressure error 112
I
index error 112
inlet module
cleaning 102
description 4
installation
checklist 10
dynamic mixer 34
leak sensor 87
seal wash pump 44
serial communication cable 17
system interconnect cable 18
unpacking 11
instrument casing, cleaning 99
interconnect cable, installation 18
L
LDA
assembling 84
dismantling 81
functional description 4
installing 87
removing 75
leak sensor
actions, setting up in the Xcalibur data system 70
configuration, ChromQuest data system 63
installation 49
ordering information 129
132
Accela 600 and 1250 Pump Hardware Manual
LED, status 6
liquid displacement assembly (LDA) 4
low-pressure error 112
low-pressure solvent lines 24
M
maintenance schedule 74
motor error 112
O
over voltage category xvii
P
PDA detector, interconnect cable connection 19
piston chambers 4
piston guide bushings, flushing 57, 100
pistons, cleaning 98
platform, solvent 25
plugs, international 16
Pollution Category 2 xvii
position error 112
power cord, connecting 16
primary piston seals, replacing 92
proportioning accuracy, testing 108
proportioning valves 4
pulsation, testing 107
pump
environmental requirements 11
functional description 2
interconnect cable connection 19
LDA maintenance 75
powering on 32
replaceable parts 115
seal wash (figure) 45
troubleshooting guide 113
USB connection to the data system computer 17
purge valve 4
R
regulatory compliance iii
replacement parts
inlet valve assembly 122
main body 115
residual pulsation 107
Thermo Scientific
Index: S
S
safety information xvi
safety standards iii
seal wash pump
functional description 5
installing 44
ordering information 128
seals, pump
primary, replacing 92
secondary, replacing 96
secondary piston seals, replacing 96
serial number, pump 62
solenoid error 112
solvent lines
connecting the low-pressure lines 24
non-metallic filters 24
solvent platform or tray 25
solvent requirements xviii
solvent reservoir bottles, assembling 24
solvent reservoir filter 25
space requirements 11–14
start cable, dual-pump system 20
T
tray, solvent 25
troubleshooting guide
degasser 114
pump 113
U
unpacking, shipping container 11
USB cable, port, and plug 17
V
vacuum degasser, description 4
valves
injection, Accela Open Autosampler 31
purge, pump 4
W
WEEE compliance vii
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
133
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