Ocean Optics HR4000 Series Installation and Operation Manual
Ocean Optics HR4000CG-UV-NIR Series spectrometers are high-resolution fiber optic spectrometers designed for advanced spectroscopy applications. With a wavelength range of 200-1100 nm, these spectrometers are ideal for a wide range of applications, including absorbance, emission, and reflectance measurements. The HR4000CG-UV-NIR Series spectrometers feature a unique combination of high resolution, sensitivity, and speed, making them ideal for applications requiring precise and accurate measurements.
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HR4000 and HR4000CG-UV-NIR
Series Spectrometers
Installation and Operation Manual
For Products: HR4000/HR4000CG-UV-NIR
Document: 210-00000-000-02-201604
000-00000-000-02-A
AMERICAS & WORLD HEADQUARTERS
Phone: +1 727-733-2447
Fax: +1 727-733-3962
Sales: [email protected]
Orders: [email protected]
Support: [email protected]
Ocean Optics, Inc.
830 Douglas Ave.
Dunedin, FL 34698
USA
Manufacturing & Logistics
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Winter Park, FL 32792
USA
EUROPE, MIDDLE EAST & AFRICA
Phone: +31 26-319-0500
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www.oceanoptics.com
Copyright © 2008 Ocean Optics, Inc.
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
Trademarks
All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their respective owners.
Limit of Liability
Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the information contained in this manual.
Table of Contents
Chapter 1: Introduction ..................................................................... 1
Chapter 2: Installing the HR4000 ...................................................... 5
Chapter 3: Troubleshooting .............................................................. 7
HR4000 Connected to Computer Prior to Operating Software Installation ..................... 7
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Table of Contents
Remove the Unknown Device from Windows Device Manager ........................................... 7
Appendix A: Calibrating the Wavelength of the HR4000 ................ 11
Appendix B: Specifications ............................................................... 15
Appendix C: HR4000CG-UV-NIR Spectrometer ............................... 24
ii 210-00000-000-02-201604
About This Manual
Document Purpose and Intended Audience
This document provides the user of HR4000 Series Spectrometers (both HR4000 and
HR4000CG-UV-NIR) with instructions for setting up, calibrating and performing experiments with their spectrometer.
What’s New in this Document
This version of the HR4000 and HR4000CG-UV-NIR Series High-Resolution Fiber Optic Spectrometers
HR4000 / HR4000CG-UV-NIR Installation and Operation Manual updates the specifications.
Document Summary
Chapter
Description
Contains descriptive information about the HR4000
Spectrometer and how sampling works. It also provides a list of system requirements, interface options, and shipment components.
Chapter 2: Installing the HR4000
Provides installation and configuration instructions.
Contains recommended steps to isolate and correct common problems.
Provides instructions for calibrating the HR4000 Series
Spectrometer.
Contains technical specifications and connector pinouts for the
HR4000 Series Spectrometer.
Contains features and specifications unique to the
HR4000CG-UV-NIR Spectrometer.
Product-Related Documentation
You can access documentation for Ocean Optics products by visiting our website at http://www.oceanoptics.com
. Select Technical Operating Instructions, then choose the appropriate document from the available drop-down lists.
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iv
About This Manual
Document for… Document Location
OceanView software http://oceanoptics.com///wp-content/uploads/OceanViewIO.pdf
SpectraSuite software http://oceanoptics.com///wp-content/uploads/SpectraSuite.pdf
HR-4 Breakout Box http://oceanoptics.com///wp-content/uploads/HR-4-Breakout-Box.pdf
External triggering for firmware versions below 3.0 http://oceanoptics.com///wp-content/uploads/External-Triggering-Options.pdf
External triggering for firmware versions 3.0 and above http://oceanoptics.com///wp-content/uploads/External-Triggering-
Options_Firmware3.0andAbove.pdf
Ocean Optics offers a Glossary of spectroscopy terms to help you further understand your state-of-the-art products and how they function, located at: http://oceanoptics.com/glossary/.
Upgrades
Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a product.
Warranty
Our 3-Year Warranty covers Ocean Optics miniature fiber optic spectrometers, light sources and sampling accessories – regardless of the application – from manufacturing defects. It also covers fibers and probes for a full 12 months: http://oceanoptics.com/services/exclusive-3-year-warranty/
This comprehensive warranty ensures you of the highest level of craftsmanship and reliability for years to come. No other manufacturer offers such a solid guarantee of quality and reliability.
The Ocean Optics 3-Year Warranty applies to Ocean Optics equipment (excluding OEM configurations) purchased on or after July 1, 2010. The warranty covers parts and labor needed to repair manufacturing defects that occur during the warranty period. We also will cover the costs of shipping warranty-related repairs from our customers to Ocean Optics and from us to our customers.
WARNING
This is a Class A product. In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures.
210-00000-000-02-201604
About This Manual
ISO Certification
Ocean Optics, the industry leader in miniature photonics, has been certified for ISO 9001:2008 certification applicable to the design and manufacture of electro-optical equipment since 2009.
WARNING
This is a Class A product. In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures.
FCC COMPLIANCE
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which the user will be required to correct the interference at his own expense.
WARNING: The authority to operate this equipment is conditioned by the requirement that no modifications will be made to the equipment unless the changes or modifications are expressly approved by the manufacturer.
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About This Manual vi 210-00000-000-02-201604
Chapter 1
Introduction
Product Overview
The HR4000 High-Resolution Miniature Fiber Optic Spectrometer provides optical resolution as good as
0.025 nm (FWHM). The HR4000 is responsive from 200-1100 nm, but the specific range and resolution depends on your grating and entrance slit selections.
The HR4000 is perfect for applications where high resolution is necessary, such as absorbance of gases or atomic emission lines (for solution chemistry or for color measurements, the USB4000 is more appropriate).
Data programmed into a memory chip on each HR4000 includes wavelength calibration coefficients, linearity coefficients, and the serial number unique to each spectrometer. Our spectrometer operating software simply reads these values from the spectrometer — a feature that enables hot swapping of spectrometers among computers.
The HR4000 Spectrometer connects to a notebook or desktop computer via USB port or serial port. When connected to the USB port of a computer, the HR4000 draws power from the host computer, eliminating the need for an external power supply. The HR4000, like all our USB devices, can be controlled by our
OceanView software, a Java-based spectroscopy software platform that operates on Windows, Macintosh and Linux operating systems.
Ocean Optics HR4000 High-Resolution Fiber Optic Spectrometer
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2
1: Introduction
System Requirements
You can use the HR4000’s USB connectivity with any computer that meets the following requirements:
Microsoft Windows – Windows 2000/XP/7/10; 32-bit and 64-bit and Windows Vista (32-bit only)
Apple Macintosh –version 10.5 Intel or later
Linux – Any version released for an x86 or amd64 platform since 2010
Alternately, the HR4000 has serial port adaptability for connecting to PCs, PLCs, and other devices that support the RS-232 communication protocol. However, this connection method requires an external power supply to power the HR4000, the Breakout Box (HR4-BREAKOUT), and a serial cable.
EEPROM Utilization
An EEPROM memory chip in each HR4000 contains wavelength calibration coefficients, linearity coefficients, and a serial number unique to each individual spectrometer. The software application reads these values directly from the spectrometer, enabling the ability to “hot-swap” spectrometers between PCs without entering the spectrometer coefficients manually on each PC.
About OceanView Software
OceanView is the latest generation of operating software for all Ocean Optics spectrometers. It is a completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device.
OceanView is a user-customizable, advanced acquisition and display program that provides a real-time interface to a variety of signal-processing functions. With OceanView, you have the ability to perform spectroscopic measurements (such as absorbance, reflectance, and emission), control all system parameters, collect and display data in real time, and perform reference monitoring and time acquisition experiments. Consult the OceanView manual for hardware requirements when using OceanView (see
Documentation
Sampling System Overview
How Sampling Works
Ocean Optics components function in a sampling system as follows:
1. The user stores reference and dark measurements to correct for instrument response variables.
2. The light from the light source transmits through an optical fiber to the sample.
3. The light interacts with the sample.
4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.
5. The spectrometer measures the amount of light and transforms the data collected by the spectrometer into digital information.
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1: Introduction
6. The spectrometer passes the sample information to spectrometer operating software.
7. The software compares the sample to the reference measurement and displays processed spectral information.
Modular Sampling Accessories
Ocean Optics offers a complete line of spectroscopic accessories for use with the HR4000. Most of our spectroscopic accessories have SMA connectors for application flexibility. Accordingly, changing the sampling system components is as easy as unscrewing a connector and replacing an accessory.
Interface Options
The HR4000 has both USB and serial port connectors (with the use of an adapter), enabling you to connect the spectrometer to a desktop or notebook PC via a USB port. However, you must create custom software if using the serial port. OceanView software is available if you are connecting via the USB port.
Breakout Box
Ocean Optics also offers the Breakout Box (HR4-BREAKOUT), a passive module that separates the signals from their 30-pin port to an array of standard connectors and headers, enabling easy access to a variety of features found in Ocean Optics’ HR4000 Spectrometer. In addition to the accessory connector, the breakout box features a circuit board based on a neutral breadboard pattern that allows custom circuitry to be prototyped on the board itself.
Note
The HR4000 Breakout Box is compatible with HR4000 Spectrometers with Revision B or greater. HR4000 Spectrometers with serial numbers beginning with HR4A are not compatible with the HR4000 Breakout Box.
Shipment Components
The following information and documentation ships with the HR4000 Spectrometer:
Packing List
The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice arrives separately). It lists all items in the order, including customized components in the spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes the shipping and billing addresses, as well as any items on back order.
Wavelength Calibration Data Sheet
Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information unique to your spectrometer. OceanView reads this calibration data from your spectrometer when it interfaces to a computer via the USB port.
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1: Introduction
Note
Please save the Wavelength Calibration Data Sheet for future reference.
Other Accessories Available
Visit us at www.OceanOptics.com
for a complete list of products available for all of your spectroscopy needs.
Fibers
Light Sources
Integrated Sampling Systems
Cuvettes
Filter Holders
Lithium Ion Battery Pack
HR4-BREAKOUT Breakout Box
4 210-00000-000-02-201604
Chapter 2
Installing the HR4000
Overview
You must install the operating software application prior to connecting the HR4000 Spectrometer to the computer. The Ocean Optics spectrometer operating software installs the drivers required for the HR4000 spectrometer installation. If you do not install the software first, the system will not properly recognize the HR4000.
If you have already connected the HR4000 to the computer prior to installing the operating software,
consult Chapter 3: Troubleshooting for information on correcting a corrupt HR4000 installation.
HR4000 Installation
This section contains instructions for connecting the HR4000 via both USB and serial modes.
USB Mode
Note
The USB port on a computer can power up to five HR4000 spectrometer channels.
Systems with more than five channels require a powered USB hub.
► Procedure
Follow the steps below to connect the HR4000 to a computer via the USB port:
1. Install the spectrometer operating software on the destination computer.
2. Locate the USB cable (USB-CBL-1) provided with the HR4000.
3. Insert the square end of the cable into the side of the HR4000.
4. Insert the rectangular end of the cable into the USB port of the PC.
If you installed the spectrometer operating software prior to connecting the HR4000, the software installs the HR4000 drivers. If the drivers do not successfully install (or if you connected the HR4000 to the
computer before installing the software), consult Chapter 3: Troubleshooting .
Once you install the software and hardware, and establish your sampling system, you are ready to take measurements.
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2: Installing the HR4000
Serial Port Mode
To use the serial port capacity of the HR4000 Spectrometer, the PC must be running a 32-bit version of the Windows operating system.
► Procedure
Follow the steps below to connect the HR4000 to the PC via serial port:
1. Connect the serial cable adapter block to the appropriate pins of the HR4000’s 30-Pin Accessory
Connector.
2. Connect one end of the 9-pin serial cable to the adapter block on the HR4000, and then connect the other end to a serial port on the PC.
3. Note the number of the serial port (COM Port) to which you connected the HR4000 (some PCs may not have numbered ports; handheld PCs typically have only one serial port).
4. Plug the 5 VDC external power supply into an outlet and connect it to the HR4000.
Connect Spectroscopic Accessories
To find operating instructions for HR2000+-compatible products (such as light sources, sampling chambers, and probes), consult the Ocean Optics website at http://oceanoptics.com/support/technicaldocuments/.
HR4000 Configuration
The HR4000 can be used with OceanView operating software when connected to the USB port.
If you have followed the previous steps and started OceanView, the spectrometer is already acquiring data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light intensity. This means the software and hardware are correctly installed.
External Triggering Options
You can trigger the HR4000 using a variety of External Triggering options through the 30-pin
Accessory Connector on the spectrometer. For HR4000 firmware versions below 3.0, see the
External Triggering Options document located at
http://oceanoptics.com///wpcontent/uploads/External-Triggering-Options.pdf
.
For HR4000 firmware version 3.0 and above, see the
External Triggering Options document located at http://oceanoptics.com///wp-content/uploads/External-
Triggering-Options_Firmware3.0andAbove.pdf
.
The triggering document contains instructions for configuring External Triggering options for the HR4000.
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Chapter 3
Troubleshooting
Overview
The following sections contain information on troubleshooting issues you may encounter when using the
HR4000 Spectrometer.
HR4000 Connected to Computer Prior to
Operating Software Installation
Windows Operating Systems
If you connected your Ocean Optics HR4000 device to the computer prior to installing your spectrometer operating software application, you may encounter installation issues that you must correct before your
Ocean Optics device will operate properly.
Follow the applicable steps below to remove the incorrectly installed device, device driver, and installation files.
Note
If these procedures do not correct your device driver problem, you must obtain the
Correcting Device Driver Issues document from the Ocean Optics website: http://oceanoptics.com///wp-content/uploads/Correcting-Device-Driver-Issues.pdf
Remove the Unknown Device from Windows Device Manager
► Procedure
1. Open Windows Device Manager. Consult the Windows operating instructions for your computer for directions, if needed.
2. Locate the Other Devices option and expand the Other Devices selection by clicking on the "+" sign to the immediate left.
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3: Troubleshooting
Note
Improperly installed USB devices can also appear under the Universal Serial Bus
Controller option. Be sure to check this location if you cannot locate the unknown device.
3. Locate the unknown device (marked with a large question mark). Right-click on the Unknown
Device listing and select the Uninstall or Remove option.
4. Click the OK button to continue. A warning box appears confirming the removal of the Unknown
Device. Click the OK button to confirm the device removal.
5. Disconnect the HR4000 from your computer.
6. Locate the section in this chapter that is appropriate to your operating system and perform the
steps in the following Remove Improperly Installed Files section.
Remove Improperly Installed Files
► Procedure
1. Open Windows Explorer.
2. Navigate to the Windows | INF directory.
Note
If the INF directory is not visible, you must disable the Hide System Files and Folders and Hide File Extensions for Known File Types options in Windows Folder Options.
Access Windows Folder Options from Windows Explorer, under the Tools | Folder
Options menu selection.
3. Delete the OOI_USB.INF in the INF directory. If your computer is running either the Windows
2000 or XP operating system, you must also delete the OOI_USB.PNF file in the INF directory.
4. Navigate to the Windows | System32 | Drivers directory.
5. Delete the EZUSB.SYS file.
6. Reinstall your Ocean Optics application and reboot the system when prompted.
7. Plug in the USB device.
The system is now able to locate and install the correct drivers for the USB device.
Mac Operating Systems
Since there are no device files for the HR4000 Spectrometer in a Mac operating system, you should not encounter any problems if you installed the spectrometer before the spectrometer operating software.
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3: Troubleshooting
Linux Operating Systems
For Linux operating systems, all you need to do is install the spectrometer operating software, then unplug and replug in the spectrometer. Technically, the driver files for Linux simply give nonprivileged users permission to use newly connected hardware. There isn’t any long-term harm to plugging in the device before installing the software.
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3: Troubleshooting
10 210-00000-000-02-201604
Appendix A
Calibrating the Wavelength of the HR4000
Overview
This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the
HR4000.
About Wavelength Calibration
You are going to be solving the following equation, which shows that the relationship between pixel number and wavelength is a third-order polynomial:
p
= I + C
1
p + C
2 p
2
+ C
3 p
3
Where:
= the wavelength of pixel p
I = the wavelength of pixel 0
C
1
= the first coefficient (nm/pixel)
C
2
= the second coefficient (nm/pixel
2
)
C
3
= the third coefficient (nm/pixel
3
)
R
= the reference intensity at wavelength
You will be calculating the value for I and the three Cs.
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A: Calibrating the Wavelength of the HR4000
Calibrating the Spectrometer
Preparing for Calibration
To recalibrate the wavelength of your spectrometer, you need the following components:
A light source capable of producing spectral lines
Note
Ocean Optics’ HG-1 Mercury-Argon lamp is ideal for recalibration. If you do not have an
HG-1, you need a light source that produces several (at least 4-6) spectral lines in the wavelength region of your spectrometer.
An HR4000 spectrometer
An optical fiber (for spectrometers without a built-in slit, a 50 m fiber works best)
A spreadsheet program (Excel or Quattro Pro, for example) or a calculator that performs thirdorder linear regressions
Note
If you are using Microsoft Excel, choose Tools | Add-Ins and check AnalysisToolPak and AnalysisTookPak-VBA.
Calibrating the Wavelength of the Spectrometer
► Procedure
Perform the steps below to calibrate the wavelength of the spectrometer:
1. Place the operating software into Quick View (Scope) mode and take a spectrum of your light source. Adjust the integration time (or the A/D conversion frequency) until there are several peaks on the screen that are not off-scale.
2. Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum intensity.
3. Record the pixel number that is displayed in the status bar or legend (located beneath the graph).
Repeat this step for all of the peaks in your spectrum.
4. Use the spreadsheet program or calculator to create a table like the one shown in the following figure. In the first column, place the exact or true wavelength of the spectral lines that you used.
In the second column of this worksheet, place the observed pixel number. In the third column, calculate the pixel number squared, and in the fourth column, calculate the pixel number cubed.
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Independent
Variable
Dependent
Variables
A: Calibrating the Wavelength of the HR4000
Values Computed from the Regression
Output
True Wavelength (nm)
253.65
296.73
302.15
313.16
334.15
365.02
404.66
407.78
435.84
546.07
576.96
579.07
696.54
706.72
727.29
738.40
751.47
Pixel #
175
296
1116
1122
1491
1523
1590
1627
1669
312
342
402
490
604
613
694
1022
Pixel #
2
30625
87616
97344
116964
161604
240100
364816
375769
481636
1044484
1245456
1258884
2223081
2319529
2528100
2647129
2785561
Pixel #
3
5359375
25934336
30371328
40001688
64964808
117649000
220348864
230346397
334255384
1067462648
1389928896
1412467848
3314613771
3532642667
4019679000
4306878883
4649101309
Predicted
Wavelength
253.56
296.72
302.40
313.02
334.19
365.05
404.67
407.78
435.65
546.13
577.05
579.01
696.70
706.62
727.24
738.53
751.27
5. Use the spreadsheet or calculator to calculate the wavelength calibration coefficients. In the spreadsheet program, find the functions to perform linear regressions.
If using Quattro Pro, look under Tools | Advanced Math
If using Excel, look under Analysis ToolPak
6. Select the true wavelength as the dependent variable (Y). Select the pixel number, pixel number squared, and the pixel number cubed as the independent variables (X). After executing the regression, you will obtain an output similar to the one shown below. Numbers of importance are noted.
Regression Statistics
Multiple R
R Square
0.999999831
0.999999663
Adjusted R Square 0.999999607
Standard Error 0.125540214
Observations 22
R Squared
Intercept
Coefficients Standard Error
Intercept 190.473993
X Variable 1 0.36263983
0.369047536
0.001684745
X Variable 2-1.174416E-05 8.35279E-07
X Variable 3-2.523787E-09 2.656608E-10
Third coefficient
First coefficient
Second coefficient
Difference
0.09
0.01
-0.09
0.06
-0.15
0.10
0.06
-0.13
0.19
-0.25
0.13
-0.05
-0.04
-0.01
0.00
0.19
-0.06
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A: Calibrating the Wavelength of the HR4000
7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at the value for R squared. It should be very close to 1. If not, you have most likely assigned one of your wavelengths incorrectly.
Keep these values at hand.
Saving the New Calibration Coefficients: USB
Mode
Ocean Optics programs wavelength calibration coefficients unique to each HR4000 onto an EEPROM memory chip in the HR4000.
You can overwrite old calibration coefficients on the EEPROM if you are using the HR4000 via the USB port. If you are using the HR4000 via the serial port, consult the Error! Reference source not found.
section later in this appendix.
► Procedure
To save wavelength calibration coefficients using the USB mode, perform the following steps:
1. Ensure that the HR4000 is connected to the PC and that you have closed all other applications.
2. Point your browser to http://oceanoptics.com/support/software-downloads/ and scroll down to
Microcode. Select USB EEPROM Programmer for your computer version.
3. Save the setup file to your computer.
4. Run the Setup.exe file to install the software. The Welcome screen appears.
5. Click the Next button. The Destination Location screen appears.
6. Accept the default installation location, or click the Browse button to specify a directory. Then, click the Next button. The Program Manager Group screen appears.
7. Click the Next button. The Start Installation screen appears.
8. Click the Next button to begin the installation. Once the installation finishes, the Installation
Complete screen appears.
9. Click the Finish button and reboot the computer when prompted.
10. Navigate to the USB EEPROM Programmer from the Start menu and run the software.
11. Click on the desired HR4000 device displayed in the left pane of the USB Programmer screen.
12. Double-click on each of the calibration coefficients displayed in the right pane of the USB
Programmer screen and enter the new values acquired in Steps 5 and 6 of the Calibrating the
Wavelength of the Spectrometer section in this appendix.
13. Repeat Step 12 for all of the new values.
14. Click on the Save All Values button to save the information, and then Exit the USB Programmer software.
The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the
HR4000.
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Appendix B
Specifications
Overview
This appendix contains information on spectrometer operation, specifications, and system compatibility.
It also includes accessory connector pinout diagrams and pin-specific information.
How the HR4000 Works
Below is a diagram of how light moves through the optical bench of an HR4000 Spectrometer. The optical bench has no moving parts that can wear or break; all the components are fixed in place at the time of manufacture. Items with an asterisk (*) are user-specified.
HR4000 Spectrometer with Components
See HR4000 Components Table on the following page for an explanation of the function of each
numbered component in the HR4000 Spectrometer in this diagram.
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B: HR4000 Specifications
HR4000 Components Table
Ocean Optics permanently secures all components in the HR4000 at the time of manufacture. Only Ocean
Optics technicians can replace interchangeable components, where noted.
Item Name Description
1
2
3
4
5
6
7
8
SMA
Connector
Secures the input fiber to the spectrometer. Light from the input fiber enters the optical bench through this connector.
Slit
Filter
A dark piece of material containing a rectangular aperture, which is mounted directly behind the SMA Connector. The size of the aperture regulates the amount of light that enters the optical bench and controls spectral resolution.
You can also use the HR4000 without a Slit. In this configuration, the diameter of the fiber connected to the HR4000 determines the size of the entrance aperture.
Only Ocean Optics technicians can change the Slit.
Restricts optical radiation to pre-determined wavelength regions. Light passes through the Filter before entering the optical bench. Both bandpass and longpass filters are available to restrict radiation to certain wavelength regions.
Only Ocean Optics technicians can change the Filter.
Collimating
Mirror
Focuses light entering the optical bench towards the Grating of the spectrometer.
Light enters the spectrometer, passes through the SMA Connector, Slit, and Filter, and then reflects off the Collimating Mirror onto the Grating.
Grating
Focusing
Mirror
Diffracts light from the Collimating Mirror and directs the diffracted light onto the
Focusing Mirror. Gratings are available in different groove densities, allowing you to specify wavelength coverage and resolution in the spectrometer.
Only Ocean Optics technicians can change the Grating.
Receives light reflected from the Grating and focuses the light onto the CCD
Detector or L2 Detector Collection Lens (depending on the spectrometer configuration).
L2 Detector
Collection
Lens
An optional component that attaches to the CCD Detector. It focuses light from a tall slit onto the shorter CCD Detector elements.
The L2 Detector Collection Lens should be used with large diameter slits or in applications with low light levels. It also improves efficiency by reducing the effects of stray light.
Only Ocean Optics technicians can add or remove the L2 Detection Collection Lens.
CCD
Detector
(UV or VIS)
Collects the light received from the Focusing Mirror or L2 Detector Collection Lens and converts the optical signal to a digital signal.
Each pixel on the CCD Detector responds to the wavelength of light that strikes it, creating a digital response. The spectrometer then transmits the digital signal to the spectrometer operating software application.
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B: HR4000 Specifications
HR4000 Specifications
The following sections provide specification information for the CCD detector in the HR4000, as well as the HR4000 Spectrometer itself. HR4000CG-UV-NIR specifications are listed in Appendix C:
HR4000CG-UV-NIR Spectrometer .
CCD Detector Specifications
Specification
Detector
No. of elements
Sensitivity
Pixel size
Pixel well depth
Signal-to-noise ratio
A/D resolution
Dark noise
Corrected linearity
Maximum pixel rate
HR4000 Spectrometer
Value
Toshiba TCD1304AP linear CCD array
3648 pixels
100 photons per count at 800 nm
8 µm x 200 µm
-100,000 electrons
300:1 (at full signal)
14 bit
8 RMS counts
>99.8%
Rate at which pixels are digitized is 1 MHz
Specification
Dimensions
Weight
Power consumption
Detector
Detector range
Gratings
Entrance aperture
Value
148.6 mm x 104.8 mm x 45.1 mm
570 g
450 mA @ 5 VDC
3648-element linear silicon CCD array
200-1100 nm
14 gratings available
5, 10, 25, 50, 100 or 200 µm wide slits
210-00000-000-02-201604 17
B: HR4000 Specifications
Specification
Order-sorting filters
Focal length
Optical resolution
Dynamic range
Fiber optic connector
Data transfer rate
Integration time
Interfaces
Operating systems:
USB Port
Serial Port
Onboard GPIO
Analog channels
Value
Installed longpass and bandpass filters f/4, 101 mm
Depends on grating and size of entrance aperture
2 x 10
9
(system); 2000:1 for a single acquisition
SMA 905 to single-strand optical fiber (0.22 NA)
Full scans into memory every 4 milliseconds with USB 2.0 port, every 600 milliseconds with the serial port
3.8 ms to 10 seconds
USB 2.0, 480 Mbps (USB 1.1 compatible); RS-232 (2-wire); SPI
(3-wire); I
2
C Inter-Integrated Circuit 2-wire serial bus
Windows 7/Vista/XP, Mac OS X, and Linux
Any 32-bit Windows operating system
10 user-programmable digital I/Os
One 13-bit analog input and one 9-bit analog output
System Compatibility
The following sections provide information on hardware and software requirements for the HR4000.
Compatibility for Desktop or Notebook PCs
To use the HR4000, you must have a PC that meets the following minimum requirements:
Microsoft Windows: Windows 2000/XP/7; 32-bit and 64-bit and Windows Vista (32-bit only)
Apple Macintosh: OS X version 10.0 or later
Linux: Any version released for an x86 or amd64 platform since 2010
Computer interfaces: USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115.2 K baud
Peripheral interfaces: I 2
C inter-integrated circuit
18 210-00000-000-02-201604
B: HR4000 Specifications
30-Pin Accessory Connector Pinout
The HR4000 features a 30-pin Accessory Connector, located on the side of the unit as shown:
30-Pin Connector
Location of HR4000 30-Pin Accessory Connector
30-Pin Accessory Connector Pinout Diagram
When facing the 30-pin Accessory Connector on the front of the vertical wall of the HR4000, pin numbering is as follows:
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
USB
Port
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29
30-Pin Accessory Connector Pinout Diagram
30-Pin Accessory Connector – Pin Definitions and
Descriptions
The following table contains information regarding the function of each pin in the HR4000’s 30-Pin
Accessory Connector:
Pin
#
Function Input/Output Description
1
2
RS232 Rx Input
RS232 Tx Output
RS232 receive signal – Communicates with a PC over DB9 Pin 3
RS232 transmit signal – Communicates with a PC over DB9 Pin 2
3
4
5
GPIO (2) Input/Output
V5_SW Output
General purpose software-programmable, digital input/output
(channel number)
Regulated 5 Volt power pin – Supplies 50 mA (maximum)
Ground Input/Output Ground
210-00000-000-02-201604 19
B: HR4000 Specifications
Pin
#
6
Function Input/Output Description
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
I
2
C SCL Input/Output I
2
C clock signal for communication to other I
2
C peripherals
I
GPIO (0)
2
C SDA
Input/Output
General purpose software-programmable, digital input/output
(channel number)
Input/Output I
2
C data signal for communication to other I
2
C peripherals
GPIO (1) Input/Output
Ext.
Trigger In
Input
General purpose software-programmable, digital input/output
(channel number)
TTL input trigger signal – See External Triggering Options document for info.
GPIO (3) Input/Output
V
CC
, V or 5V
USB
IN
, Input or
Output
General purpose software-programmable, digital input/output
(channel number)
Input power pin for HR4000 – When operating via USB, this pin can power other peripherals – Ensure that peripherals comply with USB specifications
SPI Data
Out
V
CC
, V or 5V
USB
IN
,
Output
Input or
Output
SPI Master Out Slave In (MOSI) signal for communication to other
SPI peripherals
Input power pin for HR4000 – When operating via USB, this pin can power other peripherals – Ensure that peripherals comply with USB specifications
SPI Master In Slave Out (MISO) signal for communication to other
SPI peripherals
SPI Data
In
Input
GPIO (4) Input /Output
General purpose software-programmable, digital input/output
(channel number)
Single
Strobe
Output
TTL output pulse used as a strobe signal – Has a programmable delay relative to the beginning of the spectrometer integration period
GPIO (5) Input/Output
General purpose software-programmable, digital input/output
(channel number)
SPI Clock Output
Continuou s Strobe
Output
SPI clock signal for communication to other SPI peripherals
TTL output signal used to pulse a strobe – Divided down from the master clock signal
SPI Chip
Select
Output
SPI Chip/Device Select signal for communication to other SPI peripherals
GPIO (6) Input/Output
General purpose software-programmable, digital input/output
(channel number)
20 210-00000-000-02-201604
Pin
#
Function Input/Output Description
23
24
25
26
27
28
29
30
B: HR4000 Specifications
Analog In
(0-5V)
Input 13-bit analog-to-digital input with a 0-5V range
Analog
Out (0-5V)
Output
Lamp
Enable
Output
9-bit programmable output voltage with a 0-5V range
TTL signal driven Active HIGH when the Lamp Enable command is sent to the spectrometer
GPIO (7) Input/Output
General purpose software-programmable, digital input/output
(channel number)
Ground Input/Output Ground
GPIO (8) Input/Output
General purpose software-programmable, digital input/output
(channel number)
Ground Input/Output Ground
GPIO (9) Input/Output
General purpose software-programmable, digital input/output
(channel number)
30-Pin J2 Accessory Connector - Part Numbers
The part numbers for the 30-pin accessory connector on the HR4000 Spectrometer are as follows:
The connector is Pak50™ model from 3M Corp. Headed Connector – Part Number
P50–030P1–RR1–TG.
The mating connector is Part Number P50–030S–TGF.
Mating the two components requires two 1.27 mm (50 mil) flat ribbon cables (3M 3365 Series is recommended).
If you are customizing your HR4000 Spectrometer system or configuring External Triggering, you may need these part numbers to complete your setup.
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B: HR4000 Specifications
Pin #
1
2
3
4
5
6
7
8
HR4000 15-Pin Accessory Cable Pinout
Description
Single_strobe
ContStrobe
V5_SW
ExtTrigIn
ExtTrigIn
GPIO-8
A_OUT
ExtTrigIn
Pin #
13
14
15
9
10
11
12
Description
GPIO-9
GND_SIGNAL
SDA
SCL
LampEnable
A_IN
GPIO-7
22 210-00000-000-02-201604
B: HR4000 Specifications
210-00000-000-02-201604 23
Appendix C
HR4000CG-UV-NIR Spectrometer
The HR4000CG-UV-NIR Composite Grating Spectrometer has a new proprietary grating and ordersorting filter to provide a 200-1100 nm wavelength range with 0.5 nm optical resolution in one spectrometer.
The HR4000-CG-UV-NIR is functionally similar to the standard HR4000 Spectrometer. Follow the
instructions in Chapter 2: Installing the HR4000 to configure the HR4000CG-UV-NIR.
HR4000CG-UV-NIR Features
The HR4000CG-UV-NIR contains the following features that differ from the standard HR4000:
New HC-1 Landis Composite Grating
The HR4000CG-UV-NIR uses the new HC-1 Landis grating designed to provide a 200-1100 nm wavelength range. The HC-1 is fixed in place at the time of manufacture.
Variable Order-Sorting Filter
The HR4000CG-UV-NIR contains a new OFLV-200-1000 variable order sorting filter to eliminate second and third order effects.
HR4000CG-UV-NIR Spectrometer Specifications
Specification
Dimensions:
Weight
Power consumption
Detector
Wavelength range
Optical resolution
Value
148.6 mm x 104.8 mm x 45.1 mm
570 g
450 mA @ 5 VDC
3648-element linear silicon CCD array
200-1100 nm
0.75 nm FWHM
210-00000-000-02-201604 24
Specification
Gratings
Entrance aperture
Order-sorting filters
Focal length
Dynamic range
Fiber optic connector
Data transfer rate
Integration time
Onboard GPIO
Analog channels
B: HR4000 Specifications
Value
HC-1, 300 lines per nm grating
5
m-wide slit
OFLV-200-1100 installed f/4, 101 mm
2 X 10
9
(system); 2000:1 for a single acquisition
SMA 905 to single-strand optical fiber (0.22 NA)
Full scans into memory every 4 milliseconds with the USB 2.0, every 18 ms with the USB 1.1, every 600 milliseconds with the serial port
Continuous – 4 milliseconds to 20 seconds
Shutter – 10 microseconds to 4 milliseconds
10 user-programmable digital I/Os
One 13-bit analog input and one 9-bit analog output
210-00000-000-02-201604 25
Numbers
15-pin accessory cable pinouts, 23
30-pin accessory connector diagram, 20 part numbers, 22 pin definitions, 20
A
accessories, 4, 6
Accessories, 3 accessory connector pinout, 20
B
breakout box, 3
C
Calibrating, iii, 11 calibration, 11 preparing for, 12 procedure, 12 calibration coefficients saving in USB mode, 14
CCD, 18
CCD Detector, 17 collimating mirror, 17 compatibility, 19
Desktop or Notebook PCs, 19
Components Table, 17
D
detector, 18
Detector Collection Lens, 17 document audience, iii
210-00000-000-02-201604
Index
purpose, iii summary, iii
E
EEPROM, 2
External Triggering, 6
F
filter, 17, 25 focusing mirror, 17
G
grating, 17
H
HC-1 Landis Composite Grating, 25
HR4000 specifications, 18
HR4000CG-UV-NIR, 25 features, 25
HR4000CG-UV-NIR Spectrometer specifications, 25
I
Installation, 5
Serial Port mode, 6
USB mode, 5 installed filter, 17
Interface, 3
ISO certification, v
L
L2 Detector Collection Lens, 17
Lens, 17
26
M
memory chip, 2 mirror, 17
O
OceanView, 2
Options
Interface, 3
P
packing list, 3 power supply (external), 2 product-related documentation, iii
S
Sampling
Accessories, 3
System, 2
Serial Port mode, 6 setup, 5 shipment components, 3 slit, 17
SMA Connector, 17 specifications, 16 detector, 18
HR4000, 18
HR4000CG-UV-NIR Spectrometer, 25 spectroscopic accessories, 6
System Requirements, 2
T
B: HR4000 Specifications
Triggering, 6 troubleshooting
Linux systems, 9
Mac systems, 8
Troubleshooting, 7
U
upgrades, iv
USB mode, 5
W
warranty, iv
Wavelength Calibration about, 11
Wavelength Calibration Data File, 3
Wavelength Calibration Data Sheet, 3 what's new, iii
210-00000-000-02-201604 27
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