Bio-Plex Assays—Washing Steps Made Easy Bio-Plex Pro

Bio-Plex Assays—Washing Steps Made Easy Bio-Plex Pro
Bio-Plex Pro™ Wash Stations
Bio-Plex Assays—Washing Steps Made Easy
®
Bio-Plex Pro wash stations eliminate manual wash steps
from Bio-Plex assays, making Bio-Plex assays as easy
as ELISAs. Benefits of Bio-Plex Pro wash stations include:
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Improved lab productivity
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Reliable and reproducible results
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Optimized on-board protocols
■■
Two models to meet your needs
Bio-Plex Pro Wash Station Selection Guide
Bio-Plex Pro Wash Station
Bio-Plex Pro II Wash Station
Bio-Plex assays (polystyrene beads)
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MicroPlex microspheres (polystyrene beads)
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Bio-Plex Pro assays (magnetic beads)
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Bio-Plex®
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Precision Pro assays (magnetic beads)
MagPlex microspheres (magnetic beads)
Performance
Bio-Plex Pro wash stations help decrease variability and
increase consistency in results between experiments.
The wash stations are specifically designed to perform
Bio-Plex assay wash steps, but are compatible with
any standard xMAP assay.
Representative results using the Bio-Plex Precision Pro human
cytokine assay. Experiments determining intra- and inter-assay variability
were carried out using the magnetic plate carrier on the Bio-Plex Pro wash
station. Intra-assay variability was calculated from triplicate experiments.
Inter-assay variability was determined from three separate experiments
performed on different days.
Human Target
Mean Observed Concentration, pg/ml
Intra-Assay Variability
IL-1b
IL-4
IL-6
IL-12 (p70)
IFN-g
TNF-a
Inter-Assay Variability
IL-1b
IL-4
IL-6
IL-12 (p70)
IFN-g
TNF-a
Standard
Deviation
%CV
6.39
13.22
465.09
10.12
25.03
11.84
0.172.63
0.594.50
13.632.93
1.14
11.29
0.863.45
0.786.63
6.23
16.68
467.62
16.34
32.40
13.91
0.406.45
0.211.28
17.123.66
1.51
9.26
2.307.11
0.221.56
Ordering Information
Catalog #
Description
Bio-Plex Pro Wash Stations
300-34376 Bio-Plex Pro Wash Station, includes magnetic plate
carrier, waste bottle, 2 buffer bottles
300-34377Bio-Plex Pro II Wash Station, includes magnetic
plate carrier, vacuum manifold plate carrier, waste
bottle, 2 buffer bottles
Bio-Plex Accessories
171-020100
Bio-Plex Handheld Magnetic Washer, includes
magnetic washer and hex tools for use in manual
wash steps for all Bio-Plex magnetic assays
171-025001*
Bio-Plex Pro Flat Bottom Plates, 40 × 96-well plates
Bulk Buffers
171-304500 Bio-Plex Wash Buffer, 1.5 L
* Required for washing Bio-Plex Pro assays or other magnetic
bead-based assays using the magnetic plate carrier.
MagPlex and xMAP are trademarks of the Luminex Corporation.
The Bio-Plex suspension array system includes fluorescently labeled
microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc.
by the Luminex Corporation.
Specifications
Magnetic field
Vacuum range*
Number of dispensing channels
Residual volume magnetic wash
Dispensing accuracy
Dispensing uniformity
Dimensions (W × D × H)
Weight
4,500 ± 200 gauss
–50 to –150 mbar
8
<4 μl
≤2%
CV ≤4% over the plate
28 × 37 × 18 cm
6.6 kg
* Available on the Bio-Plex Pro II wash station only.
Note: Additional features include plate-shaking capability and PC-based
software for custom protocol development.
Bio-Rad
Laboratories, Inc.
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 5044 5699
Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 9532 220 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666
New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04
Singapore 65 6415 3188 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 061 717 95 55
Taiwan 886 2 2578 7189 Thailand 800 88 22 88 United Kingdom 020 8328 2000
Life Science
Group
Bulletin 5703 Rev C
US/EG
11-1719
0112
Sig 1211
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