Zeiss | Axioplan Universal microscope | Axioplan 2 imaging The Universal Microscope System

Axioplan 2 imaging The Universal Microscope System
Microscopy from Carl Zeiss
Axioplan 2 imaging
The Universal Microscope System
Microscopy with
digital perfection
Axioplan 2
imaging
As we start a new millennium, we also enter a
new age of microscopy – the age of digital technology. As with many major milestones in the
history of microscopy, Carl Zeiss is at the forefront, leading the digital revolution. To meet the
challenges of this new age, we have created
Axioplan 2 imaging, a flexible microscope system designed for the future. An exciting single
solution with infinite flexibility that can be tailored to every application. Computer-controlled
or operated manually, this microscope not only
captures and stores images digitally and with
brilliant precision, it can also communicate with
other systems. Axioplan 2 imaging: the highperformance, high-flexibility microscope system
that guarantees new heights in success.
So: If you want to stay in the lead in the digital
age, you need a reliable partner. One who can
always meet the latest demanding requirements
in research and routine microscopy. One who is
always up to date with the rapid developments in
technology. With more than 150 years of experience in microscope making, with unquestioned
leadership in practice-oriented software and with
its highly competent sales and service organization, Carl Zeiss is this partner. Turn to Carl Zeiss
for solutions that ensure you accomplish the
tasks of today and tomorrow – from simple
image handling to complex image analysis problems.
Milestones
In 1847, Carl Zeiss sold his first microscope to a scientist.
Since then, the company he founded has been a leading
player in microscope development worldwide.
Time and again, Zeiss innovations have set new standards
in research and routine microscopy.
1925
The Carl Zeiss company develops
the first reflected-light microscope with
infinity optics.
1939
Lumipan
A universal microscope ushers
in the modern era in microscopy:
the Lumipan stand.
1958
Sets new standards in microscope design:
The Universal Research Microscope,
still indispensable in many laboratories.
1973
Axiomat
A completely new stand and legendary
quality: Axiomat. Over and above its
revolutionary design, it features infinity
optics for all microscopical techniques.
1986
The infinity optics principle brought to
perfection: Axioplan and Axiophot.
Technical innovations and unprecedented
Axioplan
ergonomic advantages set new standards.
2000
Subject to continuous optimization and updating, thousands of
these “pyramids” are doing excellent service around the world.
Today’s top model: Axioplan 2 imaging.
2
Axioplan 2 imaging –
Fluorescence
High performance in fluorescence
Simply brilliant:
must be expected of any micro-
The Light Trap
scope that claims to meet your
highest expectations. With its
The quality of a fluorescence im-
optics of high light transmission
age hinges on contrast, or signal-
and the precision of its innovative
to-noise ratio. However good the
mechanisms, the new Axioplan 2
light-gathering power of the ob-
imaging provides everything that
jective, the quality of the reflected
fluorescence microscopists could
light beam or the signal selectivity
ever want: Perfect, high-contrast
of the filter set may be, any com-
images and maximum system sta-
promise at the cost of contrast
bility in multichannel fluorescence
would impair the system’s detec-
applications.
tion sensitivity and consequently
the identification of image details.
Axioplan 2 imaging solves the
problem
with
the
proprietary
“Light Trap”. This unparalleled Carl
Zeiss invention with its new filter
cubes open at the rear removes
stray light from the imaging beam
path, thus substantially curtailing
background noise.
Stray radiation passes a dichroic
mirror (2), and leaves the filter cube (1)
on a direct path via the rear opening (3).
The tapered mount (4) of the filter cube
then deflects it out of the beam path (5).
The results: Substantially enhanced
contrast, images of greater definition
and brilliance, and higher sensitivity
Beam path
with Light Trap
1
3
4
4
Beam path
in a conventional reflector module
5
2
Bright And Brilliant
Perfect in every
dimension:
The new optics
The superbly corrected optics of
Axioplan 2 imaging guarantee
optimal chromatically corrected
illumination in the image plane.
Where light intensities are very
low, an optional 5-element chromatic collector further enhances
the illumination homogeneity. Improving color correction, it assures
maximum excitation and, as a
result, maximum emission. Highperformance filters made to very
close tolerances help avoid pixel
shifts. The newly designed reflected-light beam path allows the system to be tailored either for best
transmission or for diffraction-limited imaging of the field diaphragm. Thanks to interchangeable diaphragm sliders, the user
Human endothelium cells.
Quadruple fluorescence:
DAPI, Alexa 350, Alexa 488,
Phalloidin-Alexa 594.
Plan-Neofluar 63/1.25 oil.
J. Zbaeren, Insel Hospital, Bern
can select diaphragm formats or
modules.
The decisive gain in contrast:
FITC fluorescence micrograph
without Light Trap (1),
with Light Trap (2)
1
2
5
Axioplan 2 imaging –
Fluorescence
Multichannel fluorescence is play-
With 8 positions
ing an increasingly important role
in the lead :
in research, whether used alone or
Filters and filter turret
in combination with other optical
methods. There is a current trend,
Eight filter places, easily accessible
for example, from single channel
in a compact turret without com-
FISH (fluorescence in-situ hybridi-
promising the full 25 mm field of
zation) to multicolor FISH, where
view: This is top performance com-
combinations of up to seven fluo-
bined with unequalled flexibility.
rochromes yield 24 colors for auto-
You can insert a wide range of
matic chromosome analysis. In
dichroic mirror-emission filter com-
many research labs, different mu-
binations, Optovar modules and
tants of fluorescent proteins are
a DIC analyzer for convenient
combined with standard fluores-
change. If you install the optional
cent dyes. So it is becoming more
external filter changer, you can use
and more important to have six or
up to eight excitation filters as
more positions on the filter turret.
well.
With Axioplan 2 imaging, you
have eight.
With the Push&Click
mechanism, filter cubes are
changed within seconds.
Fast access to the right filter set:
the filter wheel with 8 positions
for Push&Click filter cubes
6
Number One In
Performance
And Flexibility
Fast changeover :
High-resolution four-color
banding of human chromosomes.
Dr. I. Chudoba,
Institute of Human Genetics, Jena,
and MetaSystems, Altlussheim
Push&Click
With so many options to choose
from, flexibility is the key. The new,
cube-mounted filter sets can be
quickly removed or added to the
Axioplan 2 imaging filter turret.
No more screws, no problems with
alignment: The practical Push&Click
mechanism ensures the filters are
Metaphase. Left to right:
Inverted and band-enhanced DAPI
counterstaining;
RGB presentation;
classified pseudocolor presentation
correctly
positioned
and
held
securely in place.
Chart of chromosome karyotypes.
Comparison of DAPI counterstaining
and classified chromosomes.
Dr. Köhler,
Applied Spectral Imaging
7
Axioplan 2 imaging –
Fluorescence
Research assignments pose formi-
Turn minimum signals
dable challenges to both people
into maximum results :
and
Zeiss optics
equipment.
Axioplan
2
imaging brilliantly satisfies the
Suppose you want to observe sig-
workplace. It is the researcher’s
nals at a cell membrane or flows
ideal partner when it comes to
inside a cell, or investigate the
getting the best results.
presence of GFP in, or GFP trans-
demands placed on a microscopy
Development stadia
of C. elegans. DIC.
Prof. Schnabel, Braunschweig
University of Technology
port between, specific cell compartments. No matter whether the
1–5
C. elegans. GFP-labeled
neurons.
H. Hutter, MPI Heidelberg
emission signal comes from a fluo-
1
Differentiation by
better contrast :
DIC with a memory
For studies in developmental biology using DIC microscopy, the maxi-
2
mum in resolution and contrast is
only just good enough. To achieve
this, all parts of the instrument
must be perfectly tuned to each
other – as is the case with
Axioplan 2 imaging. For every
objective, there is a specially
rescence-labeled antibody, an ion-
matched DIC slider, including one
depicting dye or another specific
with Sénarmont contrasting. Once
marker, it is too faint to be detec-
the DIC slider has been set, the
table with optics of any but the
objective will keep the setting in
highest performance. Objectives
mind.
from Carl Zeiss feature highest NA
Axioplan 2 imaging comes with
(limited only by the laws of
an extensive range of ICS objec-
physics), ideal chromatic correction
tives of renowned, continuously
and image flattening, and the very
optimized Zeiss quality, with su-
best transmission properties for
perb contrast and resolution. One
visible light, UV and IR imaging. In
of them will be ideally suited to
short: maximum performance for
your current application, whatever
every application.
it may be.
8
3
4
Focus On Research
Perfect specimen
Correct excitation :
protection :
AttoArc 2
The high-speed shutter
With AttoArc 2 you can control the
The microscopy of live objects is
intensity of the excitation light via
particularly demanding. In order to
the brightness of the lamp source.
get the best images, specimens
As a result, you automatically get
must be treated with the least
signals of the intensity needed for
possible damage. The motorized
documentation. In computer-con-
high-speed shutter of Axioplan 2
trolled multichannel fluorescence
imaging provides a superb advan-
work, the light intensity will be
tage in fluorescence work. With
automatically adjusted from chan-
shutter speeds down to a few
nel to channel. Whether with man-
microseconds, it is the perfect so-
ual or digital control, AttoArc 2
lution for GFP images of living sys-
will always provide signals of opti-
tems. Simply leave the light source
mum strength. And this means
on and control exposure time with
perfect results.
the shutter. This reduces bleaching
and phototoxicity of the specimen.
Fast but gentle :
The SoftStop function
With the new SoftStop function,
you can change reflector modules
and objectives within fractions of a
second. This will save valuable
time, especially in automated processes. In addition, a special algorithm prevents switching oscillations otherwise inevitable at such
speeds – a feature that is easy on
5
your microscope and your specimens.
Electronic dimmer
for high-pressure lamps:
AttoArc 2
9
Axioplan 2 imaging –
ICS Optics
Infinity optics were designed in the
early 1930s on the basis of August
Plan-Neofluar
objectives
Köhler’s concepts and calculations.
But it took the invention of ICS
(Infinity Color-corrected System) in
1986 to make this type of optics
the core of a complete range of
instruments.
ICS optics aim at achieving the
best possible performance with
the least possible number of optical elements. As every optical element contributes to limiting the
Fluar
objectives
system’s light transmission, minimizing the number of optical components means maximizing the
overall optical performance.
ICS optics and SI (System Integration) stand design have brought
about visible improvements in
optical performance with regard to
image contrast, brightness and
detail resolution. Today, after more
than a decade, the ICS concept is
C-Apochromat
objectives
still unsurpassed. With continued
upgrading of the imaging elements and advanced manufacturing processes, ICS optics have become indispensable for providing
the high apertures and long working distances needed for the applications of today and tomorrow.
Plan-Apochromat
objectives
10
Limited Only
By The Laws Of Physics
1) Mycelia.
Grocott silvering.
Prof. Höfler, Munich
University of Technology
2) Salivary gland of a drosophila.
GFP labeling
3) Cell nuclei and mitosis.
Triple fluorescence.
Dr. Beensen,
Friedrich Schiller University, Jena
4) Embryo of C. elegans. DIC.
Prof. Schnabel, Braunschweig
University of Technology
1
Allrounders
If flexibility and a wide range of techniques are required, universal
semi-apochromatic Plan-Neofluar objectives are the answer. With
transmission from the near UV, semi-apochromatic correction, excellent image flattening, optimum working distances, low strain and high
numerical apertures, they are ideal for brightfield, darkfield, phase
contrast, DIC, polarization and fluorescence. High contrast makes
them perfect for capturing sharp, clear images for post processing and
subsequent analysis.
2
Photon counters
The Fluar range is designed for maximum transmission and photon collection. Constructed from special optical glass, these objectives feature
high numerical apertures and high contrast, ensuring the best light
transmission throughout the visible spectral range down to the near
UV. Fluar objectives are your best choice if you want to capture even
the faintest fluorescence signal.
3
Immersion specialists
For the examination of living organisms and immersion preparations,
you need high-performance objectives capable of optically compensating differing refractive indices and volumes of the media involved.
These requirements are best met by the special family of MultiImmersion Plan Neofluar objectives or the apochromatically corrected
C-Apo family for the most demanding applications. Both will always
give you brilliant images.
4
Top performers
Thanks to the combination of best color correction with highest
numerical apertures, Plan-Apochromat objectives provide the ultimate
in resolution, image definition, and rendition of subtlest color differences. With their large apertures, they deliver outstanding brightfield
and DIC images and perform brilliantly in fluorescence microscopy.
11
Axioplan 2 imaging –
The Digital Imaging
Platform
tor, this allows you to expand your
system with video cameras, digital
compact cameras or other advanced imagers – meaning that
your system is one hundred percent future-safe.
Digital micrography :
Image quality with
AxioCam perfection
AxioCam + Axioplan 2 imaging –
12
the ideal combination of high-end
For top-quality digital images,
camera and microscope – provide
whether in color or black&white,
you with a digital imaging work-
AxioCam is the optimum imager
station unparalleled in perfection
for Axioplan 2 imaging. A camera
and convenience. No films, no time
designed by Carl Zeiss to meet the
lost with processing – the images
requirements of microscopy one
are available immediately on a PC
hundred percent. Perfect image
screen, perfectly resolved with up
quality is ensured by a sophisticated
to 3900 x 3090 non-interpolated
electronic system composed of
pixels per color channel.
first-grade devices, including a 14-
The programmable resolution of
bit analog-to-digital converter. The
AxioCam not only ensures perfect
camera is connected via glass fiber
documentation but also allows you
cable so as to be isolated from
to digitize the maximum available
ambient interferences. Cooled by a
optical resolution without any
Peltier element, the CCD image
losses. This produces brilliant digi-
sensor is kept free from disturbing
tal image files with the resolution
effects, so that even long expo-
of traditional transparencies – ideal
sures needed for very faint fluores-
for printed publications and your
cences produce images of excel-
notes, workbooks or records. Of
lent quality. AxioCam is the perfect
course, they can be incorporated
digital choice for brightfield and
into your web site or sent by e-
DIC micrography. It is amazing
mail.
how well it performs with single-
AxioCam can be used with a wide
and multichannel fluorescence; in
range of tubes and adapters for
the black&white configuration, it
micrography. Together with a use-
satisfies even the most exacting
ful device called the port replica-
demands.
Overall view
Selective enlargement
Brilliant Captures
Dual capability
Added flexibility:
with many benefits :
The dual video adapter
The multi-imaging tube
In addition to the binocular or
Want to use a video camera and a
multi-imaging tube, the dual video
digital camera in parallel? Split up
adapter provides another optical
fluorescence signals according to
port. For wavelength selection, you
wavelength and document the
can easily replace the neutral beam
image at the same time? The new
splitter with a dichroic mirror, using
motorized multi-imaging tube,
the Push&Click mechanism just as
with ports for two cameras, makes
with the multi-imaging tube.
it possible. Equipped with a soft-
That means that you can assemble
ware control interface, with XY
the exact system you need for your
camera positioning to ensure per-
specific application.
fect image overlay and with a neutral beam splitter mirror in the
Push&Click module, which you
can replace with a dichroic mirror,
the multi-imaging tube opens up
any documentation road you want
to go – with full automation.
Cat's cerebellum.
Silver staining.
J. Zbaeren,
Insel Hospital, Bern
Top:
Axioplan 2 imaging
with motorized multiimaging tube and
dual video adapter
Bottom:
Axioplan 2 imaging
with motorized
multi-imaging
tube
13
Dual TV tube
Axioplan 2 imaging –
Motorization
Reflector turret
Whether manually controlled or
Precisely in focus :
motorized, Axioplan 2 imaging
The third dimension
lets you decide precisely which
Revolving nosepiece
configuration suits your routine or
Maximum resolution governs the
research tasks best.
third dimension as well: Axioplan 2
imaging combines minute, dis-
Simple and clever :
crete focusing steps with motor-
SmartContrast
ized speed, satisfying the most
Condensers
Z con
exacting requirements of 3D imagPrecise images require precise mi-
ing. Manual focusing is supported
croscope settings. Every time you
by programmable step sizes and
change an objective or a contrast-
separate coarse and fine focusing
ing technique, you need to read-
knobs – a first for a motorized
just the light source, brightness,
microscope. Precision and repeata-
filters and diaphragms. Axioplan 2
bility at their finest!
Filter wheels and
field diaphragm
imaging saves you much of the
effort and time involved: The new
Automated convenience :
SmartContrast feature and the
Motorized
motorized DIC condenser allow you
scanning stages
to save your favored settings and
automatically retrieve them time
Motorized scanning stages open
and again – for every objective and
up almost unlimited possibilities:
every contrast technique. For ex-
Let Axioplan 2 imaging scan
ample, change from 40 x DIC to
your specimens overnight; detect,
40 x FITC – at the touch of a single
select, locate and group prede-
button!
fined patterns with the intelligent
image processing software; effect
chromosome karyotyping; produce
a high-resolution scan of your
specimen, control the microscope
remotely, or automatically track the
growth of live cells beyond the
margin of the visual field. Microscopy unlimited in time and space!
14
The graphic operator interface of AxioVision Control
Perfection
The Automatic Way
High-speed
shutter,
integrated
Personalized settings :
8-place filter wheel
The function keys
To facilitate setting, you, as well as
The motorized
components of
Axioplan 2 imaging Mot
any other user, can store up to
twenty individual, frequently used
settings in separate user menus
and activate them when needed.
trol
Select the field diaphragm setting,
the phase annulus, the objective,
and the intensity of illumination,
and save the information behind a
screen button or – with even faster
access – behind a key of your keyboard. Simply strike this key to
Supports your
Flexible choice :
reproduce the settings in next to
success :
Manual or motorized
no time. In micrography, with every
Repeatability
functions
shot you can save the exact set-
The brilliance of images is one of
It is up to you to decide which
microscope mechanisms in a data-
the two decisive quality criteria in
functions
base. Reproducibility at a key-
microscopy. Reliable repeatability
should be motorized or coded.
is the other. With its coded and
Whatever you decide or set is
motorized stands and compo-
saved by the software – whether
nents, Axioplan 2 imaging not
you make manual settings on a
only ensures exact documentation
motorized component or select a
of your experiments but also
function on the graphic user inter-
allows all settings made to be
face and see the microscope home
exactly reproduced.
in on the selected position. Here is
AxioVisionControl, a basic soft-
a concept providing you with full
ware package option available
control of everything. You decide
with the motorized Axioplan 2
whether you operate the motor-
imaging, allows you to control all
ized functions of Axioplan 2
microscope functions and inquire
imaging digitally via PC or by
all settings. Flexible, easy to oper-
hand as with a manual microscope
ate and absolutely user-friendly,
– the flexible system design gives
AxioVisionControl is also ideal in a
you the choice.
tings of all motorized and encoded
of
your
microscope
stroke – even in documentation!
multi-user environment.
15
Carl Zeiss –
Digital Technology
Computer-assisted work in micros-
Perfection the digital way:
copy has become a matter of
AxioVision
course. In fact, many applications
are inconceivable without digital
Precise image acquisition easily
help.
controlled: This is what AxioVision,
Carl Zeiss has developed a package
the Carl Zeiss imaging software
of solutions tailored to specific ap-
system, does for you. From expo-
plications. The solutions comprise
sure via processing, annotation
software and hardware modules,
and archiving to the report –
including a digital camera. Used
AxioVision reliably controls the
in conjunction with Axioplan 2
camera and the microscope, in-
imaging, the package is among
cluding the automatic storing of
the best tools available to digital
magnifications and calibration of
microscopy today.
the scaling bar when you change
objectives. The graphical user inter-
allow you to adapt AxioVision
face is easy to handle and clearly
optimally to your application. Its
laid out, with the main window
functionality can be expanded
split into a working and a docu-
easily and quickly. Supplementary
ment area. Configurable menus
modules enable added capabilities
for automatic image acquisition
techniques, thereby providing you
with the full performance spectrum of a motorized microscope –
for example: fully automatic control
in taking 3D image stacks with
motorized focusing, in multichannel
fluorescence work, time-resolved
series, or deconvolution.
Division of a
CHO cell.
Bottom left:
Original data.
Top right:
Result after
deconvolution
Intelligence
in Microscopy
Open-end system:
Software tailored to
Zeiss & partners
Axioplan applications
When special software is needed
• FISH and M-FISH image analysis
for specific research assignments,
• Telepathology and
your partners are our partners. The
telemicroscopy
open architecture of Axioplan 2
• Stereometric microscopy
imaging allows for easy linking of
• Automated 3D, 4D and
software packages from other suppliers, making this microscope the
6D imaging
• Automated assay scanning
ideal workstation for a great variety of special, software-controlled
systems (e.g. ELISPOT)
• General image acquisition
applications in many fields.
in varied fields
Optimized fluorescence :
Carl Zeiss
deconvolution software
Stray light from above or below
the focal plane is a familiar problem in fluorescence microscopy. In
extreme cases, it completely outOffering many interfaces
for the individual control of
all motorized components,
the Axioplan 2 imaging Mot
integrates easily into any system.
shines the fluorescence-labeled
structures. Carl Zeiss has solved
the problem for you: An efficient
3D deconvolution software mathematically traces the disturbing
stray light back to its origin and
thus “deconvolutes” the distorted
Video
structure. As a result, you get a
Digital imaging
Telemikroskopie
Shutter-Sync
and quality.
CAN/SUB-BUS
noise-free image of high resolution
RS 232
Internal data
Image data
Remote control data
LAN
WAN
ISDN
ATM ...
17
Laser Scanning Microscopy
Its exceptional capabilities make
Maximum comfort
Axioplan 2 imaging a perfect
with minimum effort
1
mate to the high-performance
laser scanning systems of Carl
The Carl Zeiss LSM devices based
Zeiss. Where the ultimate in reso-
on Axioplan 2 imaging are com-
lution in four or more dimensions
plete, advanced high-tech systems
is absolutely vital, LSM 510 and its
– and yet they are very easy and
“little brother”, LSM 5 PASCAL, are
convenient to use. Thanks to their
the ideal tools. Designed strictly
complete motorization, a high-
along confocal principles, they
capability image database and the
deliver first-grade optical slices of
re-use concept, you can set the
cells and tissues and thus guaran-
desired system configuration with
tee high-contrast, 3D multiple flu-
a single mouse click and start your
orescence images.
experiment – no matter whether
you use the system for one or
several applications. Flexibility combined with reproducibility!
High functionality
and freedom of choice
2
Carl Zeiss LSM devices are unequalled for flexibility with regard
to scanning with 360° field rotation, scanning zoom, offset and
such standard and special scanning
modes as spline scan or real region
of interest (rROI) scan. Short beam
paths and detectors picked for
performance make the system
extremely sensitive. In conjunction
with the precisely adjustable laser
power, this guarantees best results,
even with long-time experiments.
Moreover, the LSM multitracking
technique yields multichannel fluorescence images free from emission
crosstalk, which are ideal for colocalization measurements of, for
Your entry into
confocal microscopy:
LSM 5 PASCAL
18
example, GFP-labeled proteins.
See More,
Identify More
Large or small :
The LSM family
If you are looking for a cost-effective entry into confocal microscopy
that doesn't compromise on image
quality, LSM 5 PASCAL from Carl
1) Drosophila embryo.
Selected optical slices and
projection of the entire stack
of 47 slices
2) Section through the kidney
of an adolescent mouse.
Triple fluorescence.
3D shadow projection of a
digitally extracted renal tubule
3) Purkinje cell.
Fluorescence (Lucifer yellow)
in a vital brain section with
multiphoton excitation at 805 nm,
surface-rendered 3D projection
from 105 optical slices
Zeiss is the system of choice for
both single users or small teams. It
is versatile in use, with an emphasis on GFP applications. At the
high end of the family, LSM 510
permits the use of lasers – from UV
to the visible region to the near
infrared. A special model, LSM 510
NLO, is prepared for multiphoton
imaging and can be used for
examining thicker tissue samples
and observing live specimens in
The powerful software is constantly
3
upgraded. Installed on a high-end
PC ready for use, it offers a host of
functions for data acquisition in
one to five or more dimensions. In
addition, varied image processing
functions allow you to master
almost all applications in cell,
developmental and molecular biology, physiology, genetics and other
fields of research – no matter
whether you’re studying fixed or
living specimens.
vitro and in vivo for prolonged
times.
In combination with Axioplan 2
imaging, the LSM family comprises a perfect system focused on
your success.
19
AXIOPLAN2_E.qxd
13.03.2001
13:26 Uhr
Seite 22
System Overview
Axioplan 2 imaging
Stage carriers, Stages, Condenser carriers
Condensers
DIC-Prism I / 0.9 (with polarizer)
(000000-1005-002)
DIC-Prism II / 0.9 (with polarizer)
(000000-1005-003)
DIC-Prism III / 0.9 (with polarizer)
(000000-1005-004)
DIC-Prism II/1.4
(445375-0000-000)
DIC-Prism III/1.4
(445376-0000-000)
Achromatic-aplanatic universal condenser 0.9 H
(000000-1035-632)
Achromatic-aplanatic universal condenser 0.9 H D Ph
(445436-0000-000)
Achromatic-aplanatic universal condenser 0.9 H D Ph DIC
(445439-0000-000)
Polarizer for condensers
(000000-1028-973)
Mechanical stage 75 x 50 / 240° R with ceramic coating
(000000-1067-325)
Specimen holder for mechanical stage
75 x 50, suitable for one-hand operation
(000000-1067-330)
DIC-Prism I / 0.9
(000000-1004-901)
DIC-Prism II / 0.9
(000000-1004-902)
DIC-Prism III / 0.9
(000000-1004-903)
Specimen holder,
reflected light
(000000-1070-588)
Mechanical stage 75 x 50 R
with electric vernier scale
(000000-1046-520)
Display unit SCD
(413507-9001-000)
Universal mounting frame for
Petri dishes and specimen holder
(000000-1100-843)
Heatable universal mounting frame A-H
(000000-1116-055)
Tempocontrol 37 (1-channel)
(000000-1116-057)
Achromatic-aplanatic condenser 1.4 H Ph DIC
(445453-0000-000)
Achromatic-aplanatic universal condenser 0.9 H MOT
(445437-0000-000)
Achromatic-aplanatic universal condenser 0.9 H D Ph MOT
(445438-0000-000)
Achromatic-aplanatic universal condenser 0.9 H D Ph DIC MOT
(445440-0000-000)
Polarizer for condensers
(000000-1028-973)
Specimen holder D Pol
(45363-0000-000)
Mechanical stage 75 x 50 right
with height-adjustable drive
and friction setting
(000000-1063-835)
Specimen holder, special
(particularly for immersion objectives)
(000000-1070-589)
Specimen holder A Pol
(453564-0000-000)
Achromatic condenser 0.8 H DIC
(000000-1087-444)
DIC-Prism III / 0.8
(000000-1087-445)
Brightfield insert
(445467-0000-000)
Mounting frame for heating
and cooling devices
(000000-1107-544)
Kreuztisch 75 x 50 L
mit Ergonomietrieb
(000000-1063-836)
Mechanical stage 75 x 50
mot with control unit
(000000-1025-145)
Front optics 0.9 / dia. 2.9 Pol
(445331-0000-000)
Specimen holder
(acc. Price list)
Rotary stage Pol
(453550-0000-000)
with object guide Pol
(453560-0000-000)
Achromatic condenser 0.8 H D Ph DIC
(445445-9901-000)
DIC-Prism III / 0.8
(445485-0000-000)
optional
Joystick for 2 axes
(000000-1033-996)
or
Coaxial electronic drive
(000000-1034-960)
Achromatic-aplanatic system condenser
0.24/dia. 10.7, folded Pol
(445325-9901-000)
Stage carrier fixed with condenser
carrier “Axioplan” 2 imaging
(000000-1062-273)
Darkfield illuminator
(445314-9901-000)
Ultra condenser
1.2/1.4 (0.75-1.0)
(465500-0000-000)
20
Condenser holder Z
(445323-0000-000)
Dry darkfield condenser
0.8 / 0.95 (0.6 - 0.75)
(465505-0000-000)
왘
Condenser carrier with left and right hand controls
for vertical adjustment
(000000-1101-554)
Stage carrier, lowerable and detachable
(000000-1062-274)
21
AXIOPLAN2_E.qxd
13.03.2001
13:26 Uhr
Seite 22
System Overview
Axioplan 2 imaging
Stage carriers, Stages, Condenser carriers
Condensers
DIC-Prism I / 0.9 (with polarizer)
(000000-1005-002)
DIC-Prism II / 0.9 (with polarizer)
(000000-1005-003)
DIC-Prism III / 0.9 (with polarizer)
(000000-1005-004)
DIC-Prism II/1.4
(445375-0000-000)
DIC-Prism III/1.4
(445376-0000-000)
Achromatic-aplanatic universal condenser 0.9 H
(000000-1035-632)
Achromatic-aplanatic universal condenser 0.9 H D Ph
(445436-0000-000)
Achromatic-aplanatic universal condenser 0.9 H D Ph DIC
(445439-0000-000)
Polarizer for condensers
(000000-1028-973)
Mechanical stage 75 x 50 / 240° R with ceramic coating
(000000-1067-325)
Specimen holder for mechanical stage
75 x 50, suitable for one-hand operation
(000000-1067-330)
DIC-Prism I / 0.9
(000000-1004-901)
DIC-Prism II / 0.9
(000000-1004-902)
DIC-Prism III / 0.9
(000000-1004-903)
Specimen holder,
reflected light
(000000-1070-588)
Mechanical stage 75 x 50 R
with electric vernier scale
(000000-1046-520)
Display unit SCD
(413507-9001-000)
Universal mounting frame for
Petri dishes and specimen holder
(000000-1100-843)
Heatable universal mounting frame A-H
(000000-1116-055)
Tempocontrol 37 (1-channel)
(000000-1116-057)
Achromatic-aplanatic condenser 1.4 H Ph DIC
(445453-0000-000)
Achromatic-aplanatic universal condenser 0.9 H MOT
(445437-0000-000)
Achromatic-aplanatic universal condenser 0.9 H D Ph MOT
(445438-0000-000)
Achromatic-aplanatic universal condenser 0.9 H D Ph DIC MOT
(445440-0000-000)
Polarizer for condensers
(000000-1028-973)
Specimen holder D Pol
(45363-0000-000)
Mechanical stage 75 x 50 right
with height-adjustable drive
and friction setting
(000000-1063-835)
Specimen holder, special
(particularly for immersion objectives)
(000000-1070-589)
Specimen holder A Pol
(453564-0000-000)
Achromatic condenser 0.8 H DIC
(000000-1087-444)
DIC-Prism III / 0.8
(000000-1087-445)
Brightfield insert
(445467-0000-000)
Mounting frame for heating
and cooling devices
(000000-1107-544)
Kreuztisch 75 x 50 L
mit Ergonomietrieb
(000000-1063-836)
Mechanical stage 75 x 50
mot with control unit
(000000-1025-145)
Front optics 0.9 / dia. 2.9 Pol
(445331-0000-000)
Specimen holder
(acc. Price list)
Rotary stage Pol
(453550-0000-000)
with object guide Pol
(453560-0000-000)
Achromatic condenser 0.8 H D Ph DIC
(445445-9901-000)
DIC-Prism III / 0.8
(445485-0000-000)
optional
Joystick for 2 axes
(000000-1033-996)
or
Coaxial electronic drive
(000000-1034-960)
Achromatic-aplanatic system condenser
0.24/dia. 10.7, folded Pol
(445325-9901-000)
Stage carrier fixed with condenser
carrier “Axioplan” 2 imaging
(000000-1062-273)
Darkfield illuminator
(445314-9901-000)
Ultra condenser
1.2/1.4 (0.75-1.0)
(465500-0000-000)
20
Condenser holder Z
(445323-0000-000)
Dry darkfield condenser
0.8 / 0.95 (0.6 - 0.75)
(465505-0000-000)
왘
Condenser carrier with left and right hand controls
for vertical adjustment
(000000-1101-554)
Stage carrier, lowerable and detachable
(000000-1062-274)
21
AXIOPLAN2_E.qxd
13.03.2001
13:27 Uhr
Seite 24
Stand, Tubes, Eyepieces, Filters, Reflectors
Intermediate tubes, Nosepieses
Double video adapter
(000000-1058-640)
Binocular phototube with
height adjustment
(452146-0000-000)
Optovar intermediate tube
1.0x/1.25x/1.6x/2.0x/2.5x cod.
(452175-0000-000)
Binocular phototube 30°/25 with
sliding prism (100:0/50:50/0:100)
(452143-0000-000)
Eyepiece PI 10x/25 Br.
(444033-0000-000)
Eyepiece PI 10x/25 Br. foc
(444034-0000-000)
Eyepiece PI 16x/16 Br.
(444053-0000-000)
Eyepiece PI 16x/16 Br. foc.
(444054-0000-000)
Eyepiece E - PI 10x/25 Br. foc.
(444234-0000-000)
Eyepiece E - PI 10x/23 Br. foc.
(444235-0000-000)
Intermediate tube Pol
(with quartz depolarizer)
(452184-0000-000)
Intermediate tube for
image projection
(452181-0000-000)
Double TV-tube mot.
with 2 outputs
(000000-1054-146)
Accessories
(acc. price list
Reflector module H
(000000-1046-274)
Reflector module FI
(000000-1046-281)
Filter dia. 25 mm and
beam splitter
(acc. to price list)
왘
Binocular tube with two ports
30°/25 (100:0/50:50/0:100)
(452145-000-000)
“Axioplan” 2 imaging stand, upper part
(000000-1087-134)
“Axioplan” 2 imaging MOT stand, upper part
(000000-1079-078)
High speed shutter FL
(000000-1101-354)
Antiglare screen
(452163-000-000)
Reflected-light illuminator HD
(000000-1079-047)
Reflected-light illuminator FL
(000000-1079-073)
Reflector module H
(000000-1046-274)
Reflector module D P & C
(000000-1099-898)
Reflector module DIC
(000000-1046-277)
Reflector module DIC Rot I
(000000-1046-278)
Reflector module Pol
(000000-1046-279)
Reflector module FI
(000000-1046-281)
Optovar module 2.5x
(000000-1046-283)
Optovar module 1.6x
(000000-1046-283)
Optovar module 1.25x
(000000-1046-284)
Analyzer module D
(000000-1050-958)
Storing case for reflector modules
(000000-1103-997)
Filter slider A
(for 3 filters d = 18 mm)
(452174-0000-000)
Objective nosepiece 7x H W 0.8
(452130-0000-000)
Objective nosepiece 6x H DIC W 0.8
(452131-0000-000)
Objective nosepiece 6x H D DIC M27 cod.
(452136-0000-000)
Objective nosepiece 7x H W 0.8 mot.
(452137-0000-000)
Objective nosepiece 6x H DIC M27 mot.
(452138-0000-000)
Objective nosepiece 6x H DIC W 0.8 mot.
(000000-1067-351)
Objective nosepiece 6x Pol W 0.8 cod.
(412139-0000-000)
Iris stop slider for incident-light equipment
(000000-1062-360)
DIC slider
(acc. price list)
Built-in power supply 12 V/100 W,
stabilized 90 ... 264 V AC/50 ... 60 Hz/
225 VA, 2.5 ... 12 V DC
(000000-1079-075)
왘
왘
Transmitted-light illumination
(452150-0000-000)
Luminous field diaphragm insert D
(452160-0000-000)
Luminous field diaphragm insert D mot.
(000000-1079-076)
22
Intermediate zoom tube
1.0x ... 2.5x cod.
(452180-0000-000)
Filter magazine D
(452155-0000-000)
Filter magazine D Foto
(000000-1088-124)
Filter magazine D mot.
(000000-1079-077)
“Axioplan” 2 imaging man stand, lower part
(000000-1078-232)
“Axioplan” 2 imaging MOT stand, lower part
(000000-1078-233)
“Axioplan” 2 imaging E stand, lower part
(000000-1078-234)
Objectives
(ICS-Objectives acc. price list)
Antiflexcap for
Epiplan-Neofluar 1.25
(44921-0000-000)
Antiflexcap for
Epiplan-Neofluar 2.5
(4444922-0000-000)
Intermediate ring
M 27 on W 0.8 H = 0.94
(444911)-0000-000)
Intermediate ring
H “0” M 27 on W 0.8
(000000-1095-168)
23
AXIOPLAN2_E.qxd
13.03.2001
13:27 Uhr
Seite 24
Stand, Tubes, Eyepieces, Filters, Reflectors
Intermediate tubes, Nosepieses
Double video adapter
(000000-1058-640)
Binocular phototube with
height adjustment
(452146-0000-000)
Optovar intermediate tube
1.0x/1.25x/1.6x/2.0x/2.5x cod.
(452175-0000-000)
Binocular phototube 30°/25 with
sliding prism (100:0/50:50/0:100)
(452143-0000-000)
Eyepiece PI 10x/25 Br.
(444033-0000-000)
Eyepiece PI 10x/25 Br. foc
(444034-0000-000)
Eyepiece PI 16x/16 Br.
(444053-0000-000)
Eyepiece PI 16x/16 Br. foc.
(444054-0000-000)
Eyepiece E - PI 10x/25 Br. foc.
(444234-0000-000)
Eyepiece E - PI 10x/23 Br. foc.
(444235-0000-000)
Intermediate tube Pol
(with quartz depolarizer)
(452184-0000-000)
Intermediate tube for
image projection
(452181-0000-000)
Double TV-tube mot.
with 2 outputs
(000000-1054-146)
Accessories
(acc. price list
Reflector module H
(000000-1046-274)
Reflector module FI
(000000-1046-281)
Filter dia. 25 mm and
beam splitter
(acc. to price list)
왘
Binocular tube with two ports
30°/25 (100:0/50:50/0:100)
(452145-000-000)
“Axioplan” 2 imaging stand, upper part
(000000-1087-134)
“Axioplan” 2 imaging MOT stand, upper part
(000000-1079-078)
High speed shutter FL
(000000-1101-354)
Antiglare screen
(452163-000-000)
Reflected-light illuminator HD
(000000-1079-047)
Reflected-light illuminator FL
(000000-1079-073)
Reflector module H
(000000-1046-274)
Reflector module D P & C
(000000-1099-898)
Reflector module DIC
(000000-1046-277)
Reflector module DIC Rot I
(000000-1046-278)
Reflector module Pol
(000000-1046-279)
Reflector module FI
(000000-1046-281)
Optovar module 2.5x
(000000-1046-283)
Optovar module 1.6x
(000000-1046-283)
Optovar module 1.25x
(000000-1046-284)
Analyzer module D
(000000-1050-958)
Storing case for reflector modules
(000000-1103-997)
Filter slider A
(for 3 filters d = 18 mm)
(452174-0000-000)
Objective nosepiece 7x H W 0.8
(452130-0000-000)
Objective nosepiece 6x H DIC W 0.8
(452131-0000-000)
Objective nosepiece 6x H D DIC M27 cod.
(452136-0000-000)
Objective nosepiece 7x H W 0.8 mot.
(452137-0000-000)
Objective nosepiece 6x H DIC M27 mot.
(452138-0000-000)
Objective nosepiece 6x H DIC W 0.8 mot.
(000000-1067-351)
Objective nosepiece 6x Pol W 0.8 cod.
(412139-0000-000)
Iris stop slider for incident-light equipment
(000000-1062-360)
DIC slider
(acc. price list)
Built-in power supply 12 V/100 W,
stabilized 90 ... 264 V AC/50 ... 60 Hz/
225 VA, 2.5 ... 12 V DC
(000000-1079-075)
왘
왘
Transmitted-light illumination
(452150-0000-000)
Luminous field diaphragm insert D
(452160-0000-000)
Luminous field diaphragm insert D mot.
(000000-1079-076)
22
Intermediate zoom tube
1.0x ... 2.5x cod.
(452180-0000-000)
Filter magazine D
(452155-0000-000)
Filter magazine D Foto
(000000-1088-124)
Filter magazine D mot.
(000000-1079-077)
“Axioplan” 2 imaging man stand, lower part
(000000-1078-232)
“Axioplan” 2 imaging MOT stand, lower part
(000000-1078-233)
“Axioplan” 2 imaging E stand, lower part
(000000-1078-234)
Objectives
(ICS-Objectives acc. price list)
Antiflexcap for
Epiplan-Neofluar 1.25
(44921-0000-000)
Antiflexcap for
Epiplan-Neofluar 2.5
(4444922-0000-000)
Intermediate ring
M 27 on W 0.8 H = 0.94
(444911)-0000-000)
Intermediate ring
H “0” M 27 on W 0.8
(000000-1095-168)
23
Intermediate tubes, Illumination, Analyzers
Multi-observation equipment for “Axioskop 2”
(acc. price list)
with intermediate plate
(452968-0000-000)
100 HAL lamp housing with collector,
lamp mount and heat-reflecting filter
(447219-0000-000)
Lamp housing HBO 50 with socket
(447220-000-000)
Lamp collector HBO 50/SF 25
(447270-000-000)
Power supply unit for HBO 50,
220 ... 240 V, 50 ... 60 Hz, 350 VA
(392642-0000-000)
Path-deflecting mirror for two
illuminators
(447230-9901-000)
Path-deflecting mirror for two
illuminators (mot)
(000000-1095-490)
Bertrand lens slider Ph
(453671-0000-000)
Analyzer slider, fixed
(453657-0000-000)
Adjusting aid
HBO/XBO
(452169-0000-000)
Slider with analyzer with
rotary lambda plate
(453663-0000-000)
8-position filter wheel
for dia. 25 mm
excitation filters
(000000-1008-106)
Illuminator N HBO 103
(000000-1007-980)
Illuminator N XBO 75
(000000-1007-981)
Collector N HBO 103/XBO 75
(000000-1007-976)
Quartz collector N HBO 103/XBO 75
(000000-1007-977)
Achromatic collector
(000000-1007-978)
Super-pressure mercury lamp
HBO 103 W/2
(380301-9350-000)
XBO 75 W/2 xenon short arc lamp
(380079-9190-000)
Power supply unit for N HBO 103
90 ... 250 V, 50 ... 60 Hz, 265 VA
(000000-1003-928)
Power supply unit for N XBO 75
90 ... 250 V, 50 ... 60 Hz, 130 VA
(000000-1003-924)
Variable intensity lamp control
AttoArc 2 N HBO
(000000-1007-975)
Quartz depolarizer d = 32 mm
(453653-0000-000)
Notebook with German keyboard
(000000-1056-8525)
Notebook with US keyboard
(000000-1056-853)
100 HAL lamp housing with collector,
lamp mount and heat-reflecting filter
(447219-0000-000)
24
Software AxioVision Control
(000000-1068-563)
Equipment for Documentation
Film cassette
35 mm Mot DX
(456071-0000-000)
TV adapter for Axiophot 2
(452230-0000-000)
TV adapter 60 C 2/3˝ 1.0x
(456105-0000-000)
Camera-attachment M 4˝ x 5˝
(MC 80 DX/MC 200 CHIP)
(45060-0000-000)
(
Camera body MC 80 DX
(456031-0000-000)
Projection lens P 2.5x
(456021-0000-000)
Projection lens P 10x
(456023-0000-000)
D4 Databack
(456073-0000-000)
Adapter 60 for microskope
camera / d = 30 mm
(456006-0000-000)
Film cassette
35 mm Mot DX
(456071-0000-000)
on both sides can be
attached
TV-Camera
(as desired)
Axiophot 2 Photo module with
center-weighted averaging and spot metering
(452237-0000-000)
Adapter from 60 to 44
(456140-0000-000)
TV-Adapter
(acc. price list)
Binocular phototube 30º / 25 with
sliding prism (100:0/50:50/0:100)
(452143:0000-000)
Notebook with German keyboard
(000000-1056-852)
Notebook with US keyboard
(000000-1056-853)
Software AxioVision Control
(000000-1068-563)
Control panel MC 80 DX
(456048-0000-000)
25
Axioplan 2 imaging
Your Partner for the Future
The decision you make for a micro-
Our local consultants and techni-
scope, one equipped with the
cians and the Carl Zeiss customer
components you need, is as com-
service all support you in your
plex as the requirements it must
research with technical and appli-
meet.
cations aid, whenever you need it.
A skilled team of consultants will
Fast and reliably. Moreover, Carl
help you with making the right
Zeiss training courses and work-
choice and with budget planning.
shops provide you with added
And all of our consultants possess
insights into practical areas of
impressive know-how and experi-
microscopy and imaging tech-
ence as well as extensive knowl-
niques.
edge of the entire microscope
In fact, when you add together all
market. Thus you benefit from
the services and support that you
much more than our skill in devel-
get with Axioplan 2 imaging,
oping microscopes. You will be
you’ll see that it is more than a
able to draw upon the enormous
high-power microscope: Axioplan 2
wealth of experience that Zeiss has
imaging is your ticket to a power-
accumulated in decades of prac-
house of knowledge in microscopy
tice in research and routine. You
that has been built up over the
will profit from specific assistance
past 150 years.
in your microscopy methods – and
above all from innovative methods
which will enable you to make
great progress in your work. As a
matter of fact, you often can’t
really appreciate the full range of
Zeiss service until after you have
made your purchase – in practice,
which is what counts.
26
Convincing – from A(bbe)*
to Z(eiss)**
Accessible:
the diaphragm planes
Clearly laid out:
the AxioVisionControl software
Complete:
system solutions from a single source
Convenient:
the Push&Click filter cubes
Customized:
the modular system
Efficient:
the motorized features
Flexible:
the dual video adapter
Highly resolved:
DIC (even with Sénarmont)
Individual:
the macro function keys
Infinite:
the ICS optics
More contrast:
the Light Trap
Motorized:
the multi-imaging tube
Open-end:
the system architecture
Powerful:
the optics
Precise:
the dual-guidance stage
Retrofittable:
the confocal systems
Sturdy:
the mechanisms
Time-saving:
the Smart Contrast feature
Versatile:
8 filter positions with 25 mm field of view
Vibration-free:
the SoftStop function
Zappy:
the high-speed shutter
* Ernst Abbe (1840 –1905),
physicist and ingenious researcher,
associate of Carl Zeiss,
became sole owner in 1889.
** Carl Zeiss (1816 –1888),
mechanic, opened an optical
workshop in 1846, which grew into
a global player in optics.
27
Axioplan 2 imaging
Stand, lower part
Stand, upper part
• Manual version
• Manual version
• Coded version
• Motorized version
• Motorized version
Fluorescence
Motorization
• 25 mm field of view
• Multi-imaging tube
• Light Trap
• Reflector turret
• High-speed shutter (< 20 ms)
• Revolving nosepiece
• 8-place reflector turret
• Condensers
• Push&Click filter cubes
• Z motion with 25 nm increments
• Exchangeable field diaphragm
• Filter wheels & field diaphragm
• Adjusting aid
• High-speed shutter
• AttoArc 2
• External 8-place filter wheel
• Deflecting mirror for 2 lamps
Documentation
• Trinocular tube
• Binocular tube with 2 TV ports
• Binocular tube with 2 TV ports, motorized version
• Dual video adapter
• Axiophot 2 photomodule
485
214
304
323
376
45
385
398
491
110
315
Carl Zeiss
Light Microscopy
P.O.B. 40 41
37030 Göttingen
GERMANY
Phone : ++49 5 51 50 60 660
Telefax: ++49 5 51 50 60 464
E-Mail: micro@zeiss.de
www.zeiss.de/micro
Subject to change.
Printed on environment-friendly paper,
bleached without the use of chlorine.
For further details, please contact:
Rat tongue.
Double fluorescence
(Alexa 594, DAPI).
Plan-Apochromat 40x/1.0 oil.
J. Zbaeren, Insel Hospital, Bern
40-086 e/02.01
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