Tgradient Manual

Tgradient Manual
TGRADIENT Manual ___________________________________________________________ 1
Manual
Software Version 4.20
May 2003
Order-No.
050-800 TGradient 48
050-801 TGradient 96
Biometra
biomedizinische Analytik GmbH
Rudolf-Wissell-Str. 30, D-37079 Goettingen
Tel.: 0551/50 686-0; Fax: 0551/50 686-66
email: [email protected]
http://www.biometra.com
TGRADIENT Manual ___________________________________________________________ 2
1
Introduction
The TGRADIENT is the new high end thermocycler in the Biometra product line. It was our goal
to develop an instrument that provides fast optimization of reaction parameters as well as a high
performance for daily routine analysis. Both could be realized by a completely new block
architecture and the latest Peltier technology.
To increase the specificity of a reaction, the annealing temperature is one of the crucial
parameters. With the new TGRADIENT different annealing temperatures can be tested within a
single run. A gradient with a maximum range of 40°C can be set at any program step. We have
taken strong efforts to create a gradient as linear as possible. At the same time it was very
important to ensure homogenous temperature distribution within each row. The temperature data
for each row of the block can be retrieved through the help menu.
The new TGRADIENT is an outstanding instrument. In addition to the advanced temperature
gradient it provides high speed and reliable accuracy for routine applications. The silver block
features very high heat conductivity and ensures fast ramping speeds and a fast temperature
equilibration.
The new TGRADIENT will speed up your laboratory work because it saves time not only in
optimizing new reactions but also in your routine work.
TGRADIENT Manual ___________________________________________________________ 3
2
2.1
Before you start
Safety warnings
Before connecting the unit to the power source please ensure that the
voltage selector at the back side of the instrument is set to the required
voltage.
Danger of electric shock! Unplug the power cable before you open the
TGRADIENT Thermocycler.
It is not necessary to utilize oil into the opening of the block in order to
improve the heat transfer between the block and the sample tubes. If you
still decide to use oil, do not use silicon oil. Mineral oil may be used.
2.2
Warning symbols
The thermoblock and the heated lid will reach high temperatures
during operation. Both thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil
explosively. Always wear safety goggles during operation. Close the
lid before starting a program.
Ensure that both the rear and bottom ventilation slits of the rear and
bottom of the instrument are unobstructed. Insufficient ventilation
can cause overheating of the instrument.
TGRADIENT Manual ___________________________________________________________ 4
3
3.1
First steps with the TGRADIENT
The TGRADIENT Thermocycler front View
Knob for release
of the lid
Wheel for the height
adjustment
of the heated lid.
Keypad
Graphical display
Power switch
TGRADIENT Manual ___________________________________________________________ 5
3.2
The TGRADIENT rear view
Parallel
printer port
RS-232
port
Fuse holder
for 2 fuses,
for specifications of fuses
see type label on the
instrument´s backside
Power cord
plug
Type label including:
serial number
specifications of fuses
TGRADIENT Manual ___________________________________________________________ 6
3.3
The TGradient control panel
Graphical display
Power
Power switch:
backlit = power on
A
B
C
D
Function keys:
the corresponding functions are
specified in the screen.
7
8
9
4
5
6
1
2
3
.
0
-
Cursor keys:
For free navigation in the
programming spreadsheet.
Use to scroll through lists.
Easy to clean
keyboard
TGRADIENT Manual ___________________________________________________________ 7
3.4 Initial self test
After switching on the TGRADIENT the serial number of the instrument and the software version
is displayed.
TGradient
Serial No 1234567
Vers. 4.00 op
The TGRADIENT checks all programs in all directories (so called RAM check).
3.5 The TGradient display
The TGRADIENT display provides information about the instrument and about the status of the
active program.
prog
step
temp[°C]
time
lid
A ?
25.2°C
25.4°C
B start/stop
C programs
D +
The individual function of the special keys A , B , C and D are specified in the display and
depend on the context.
3.6 Handling of the adjustable heated Lid
To achieve optimum pressure on the tubes the TGRADIENT is equipped with an height adjustable
heated lid.
Close the lid:
After the samples have been placed in the block close the lid. Turn the wheel clockwise until you
hear a clicking noise. In this mode the pressure will not increase further, even when you keep on
turning the wheel.
Note: The pressure of the lid has been optimize for a fully loaded block. If only very few tubes
are loaded to the block you should place dummy tubes in the four corner positions to avoid
damage of tubes by excessive pressure.
Open the heated lid:
First: Release pressure by turning the wheel counter clockwise. As soon as there is no more
resistance the pressure has been released. Then: now you can open the lid with the knob.
Important: The lid should not be opened under pressure because this leads to damage of the
locking mechanism.
TGRADIENT Manual ___________________________________________________________ 8
3.7
Releasing wheel in case of blocked lid
Note: When the lid is in the extreme up or down position, it may happen that the wheel is
uncoupled. In this situation the clutch mechanism is active in both directions (clicking noise in
either direction). To unlock wheel, press metal pin with a ballpen and turn wheel carefully. This
pin overcomes the automatic clutch mechanism. Thus, care must be taken not to apply excessive
pressure.
Lid arrested in upper
position:
carefully turn wheel with
pressed pin clockwise, until
you feel normal resistance (no
more clicking noise, clutch is
released). Release pin and
turn lid down, until the clutch
mechanism is activated
(clicking noise, optimum
pressure applied).
Lid arrested in down
position:
carefully turn wheel with
pressed pin counter
clockwise, until you feel
normal resistance (no more
clicking noise, clutch is
released). Release pin and
turn wheel counter clockwise
until pressure is completely
released. Open lid.
IMPORTANT: WHEN THE CLUTCH MECHANISM IS ACTIVE (OPTIMUM
PRESSURE IS APPLIED), NEVER USE PIN TO FURTHER INCREASE PRESSURE.
THIS WILL LEAD TO DAMAGE OF TUBES AND INSTRUMENT!
TGRADIENT Manual ___________________________________________________________ 9
4
Creating a Program
4.1 Select a directory
In the TGRADIENT programs can be stored both in the main directory and in individual
subdirectories. For easy identification of the selected storage place, the subdirectories can be
named (see section 4.3).
Start with the main screen.
Prog
step
temp[°C]
time
lid
A ?
25.2°C
25.4°C
B start/stop
C programs
D +
Press [C programs] to enter the edit mode.
Main direct.--A ?
½-3.
½-4.
½-5.
½-6.
½-7.
B
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
D enter
You are now in the main directory. The structure of the subdirectories is displayed.
4.2 Select a program store
Programs can be stored in the main directory or in subdirectories.
To create a program in the main directory press [D enter].
To access a subdirectory move right with the cursor key ð and select a subdirectory with the
cursor keys ñò. The selected directory will be highlighted.
Main direct.---
A ?
½-3.
½-4.
½-5.
subdirect.:
subdirect.:
Subdirect.:
½-6.
½-7.
subdirect.:
subdirect.:
C quit
B
D enter
TGRADIENT Manual __________________________________________________________ 10
4.3 Enter name for subdirectory
The process for naming a subdirectory and a program is the same. For information about naming
programs see section 4.4.
Press [D enter] to enter the subdirectory
directory:
program no.
edit
A list
5
Name:
B
C quit
D enter
Press [A list] to view a list of all existing files and empty memory in this directory.
Note: If neither program name nor the word “empty” is displayed, this place may be occupied by
a program without name. For the programming of new protocols choose an empty memory.
Existing programs that are not needed any more can be deleted to provide new memory.
0
1
2
3
4
A
empty
empty
empty
empty
empty
list
B forward
C quit
D enter
Scroll through the list with cursor keys ñò and confirm memory with [D enter].
4.4 Enter a program name
Each program is specified by a program number and a subdirectory number. To make retrieval of
a program easier, you can enter a name for each program existing of letters, numbers and
symbols.
Directory:
program no.
A ABC
3
0
B files
Name:
Ù
ABCDEFGHIJKLMNOPQRST
UVWXYZ-()#°C/,áñ&+.%!
C name OK
D enter
To enter a name for your program press [A ABC].
Directory:
program no.
A name
3
0
B blank
Name:
Ù
ABCDEFGHIJKLMNOPQRST
UVWXYZ-()#°C/,áñ&+.%!
C quit
D enter
Choose a letter from the alphabet with cursor keys ïðñò
TGRADIENT Manual __________________________________________________________ 11
Confirm each letter with [D enter].
Directory:
program no.
A name
3
0
B blank
Name:
T
Ù
ABCDEFGHIJKLMNOPQRST
UVWXYZ-()#°C/,áñ&+.%!
C name OK
D enter
The selected letter will appear in the display and the cursor will move one step to the right.
Select the next letter and confirm with [D enter].
Note: What to do if a wrong letter has been accepted?
To alter the position of the cursor within the name press [A name]. Now you can move the cursor
with cursor keys ïð through the name. This is helpful if you have confirmed an wrong letter or
if you want to change the name of an existing program.
Once the name is complete, confirm name with [C name OK].
Directory:
program no.
lid temp:
edit
A ?
3
0
°C
B files
Name:
TEST 1
preheating: on
C pgm OK
D enter
4.5 Enter the lid temperature
You can now enter a temperature for the heated lid. Note: In general, the lid temperature should
be about 10°C above the highest temperature in the protocol. Example: If your denaturation step
is at 95°C, enter 105°C for the lid.
Directory:
3
program no.
0
lid temp:
105 °C
edit
A ?
B files
Name:
TEST 1
preheating: on
C pgm OK
D enter
4.6 Select / deselect pre-heating of the lid
You can choose whether the lid is pre-heated before the program starts. This is to avoid
condensation during the initial heating phase. Note: During the preheating of the lid, the block is
held constant at 25°C.
TGRADIENT Manual __________________________________________________________ 12
Directory:
3
program no.
0
lid temp:
105 °C
edit
A on/off
Name:
TEST 1
preheating: on
B files
C pgm OK
D enter
Note: The default setting, which is recommended for most applications, is preheating: on.
After all pre-settings have been made, press [D enter] to open the programming spreadsheet.
4.7 Enter temperature and time settings
The programming spreadsheet:
Temp[°C]
1
2:
3:
4:
A ?
time
ß
#
B insert/delete
gradient[°C]
C pgm OK
opt à
D enter
Note: In this spreadsheet you can enter all parameters for your cycling protocol. Furthermore,
you can set special parameters like touch down, ramping rates or time increments. For further
details on these special parameters, see section 5.1.
Note: You can navigate in the spreadsheet without limitations with the four cursor keys
ïðñò.
Note: Each setting is confirmed with [D enter]. The cursor moves automatically to the next field.
Alternatively, you can confirm a value by moving forward with the cursor keys.
Note: At any time you can call up the help function with [A ?]
Note: In an existing protocol program steps can be deleted and inserted. For further information
on deleting and inserting program steps see section 5.1.
Now enter temperature for the first step in the protocol:
Temp[°C]
time
ß
#
1
95,0
2:
3:
4:
A ?
B insert/delete
gradient[°C]
C pgm OK
opt à
D enter
Confirm temperature with [D enter] or just move on with the cursor key. If you move to the next
row, the setting will be stored in the temporary memory.
TGRADIENT Manual __________________________________________________________ 13
Temp[°C]
1
95.0
2:
3:
4:
A ?
time
ß
#
B insert/delete
gradient[°C]
C pgm OK
opt à
D enter
Enter a time for step one.
Note: There is a convention on how time settings are entered in all BIOMETRA cyclers:
hours l minutes l seconds
If you enter a number without “point” this value will be interpreted as seconds (“300” => 5
minutes). To program minutes enter a “l” after the number of minutes. To enter hours enter l l
after the number. You can also enter any combination of hours, minutes and seconds. Example:
for 1 hour, 30 minutes, 20 seconds enter 1l 30 l 20.
The time values will be displayed in the following format: 0h 00 m 00s
Accept time setting with [D enter] or move to the next field with cursor key.
4.8 Set temperature gradient
Note: Each temperature gradient is defined by two settings: first: the temperature in the middle
of the block, second: the range of the gradient. For example if you want to program a
temperature gradient from 50 to 60°C, enter 55°C as temperature an 10°C for the gradient.
Temp[°C]
1: 95.0
2: 95.0
3: 55.0
4:
A ?
time
0h 5m 0s
0h 0m30s
0h 0m30s
ß
#
B insert/delete
gradient[°C]
opt à
10°C
C pgm OK
D enter
Note: The maximum temperature gradient is 40°C.
Note: You can program as many steps with temperature gradient as you want.
Note: You can even set an inverse temperature gradient. An inverse gradient has the higher
temperature on the left side of the block and the lower temperature on the right side respectively.
Inverse gradient are set by adding the algebraic sign “-“ to the gradient value.
4.9 View different temperatures of the gradient in the block
To view the temperatures in the 12 different block rows press [A ?]
TGRADIENT Manual __________________________________________________________ 14
Temp[°C]
time
ß #
gradient[°C] opt à
T1 – 6: 50.0 50.2 50.9 52.0 53.2 54.4 °C
T7 –12: 55.6 56.8 58.0 59.1 59.8 60.0 °C
3: 55.0 0h 0m30s
10°C
4:
Temperature difference left side / right side
In the first highlighted lane the temperatures from row 1 to 6 are displayed. In the second
highlighted lane the temperatures from row 7 to 12 are displayed.
Note: While the TGRADIENT is cycling you can view the current gradient temperatures. The
temperatures can be displayed online during each gradient step (see section 7.4). As the program
moves to the next step the last gradient temperatures are stored. Thus you can check the
temperatures of the last gradient in consecutive steps without gradient. For further information
on the current status of the cycler see section 7.
4.10 Set cycle number
Note: In general, loops are defined by selecting the target for the back loop and the number of
backloops.
Temp[°C]
1: 95.0
2: 95.0
3: 55.0
4: 72.0
A ?
time
ß #
0h 5m 0s
0h 0m30s
0h 0m30s
0h 0m30s
2
B insert/delete
gradient[°C]
C pgm OK
opt à
D enter
In the field labeled with ß you can select the target step for a backloop.
Temp[°C]
1: 95.0
2: 95.0
3: 55.0
4: 72.0
A ?
time
ß #
gradient[°C] opt à
0h 5m 0s
0h 0m30s
0h 0m30s
0h 0m30s 2
29
B insert/delete
C pgm OK
D enter
Enter the number of backloops. Note: total cycler number = (n backloops) + 1, e.g. enter 29 for a
total number of 30 cycles.
4.11 Cool below ambient temperature
Temp[°C]
2: 95.0
3: 55.0
4: 72.0
5: 4.0
A ?
time
ß #
gradient[°C] opt à
0h 0m30s
0h 0m30s
0h 0m30s 2
29
pause
B insert/delete
C pgm OK
D enter
To hold the temperature for an indefinite time enter “0”. After pressing [D enter] “pause” is
displayed.
TGRADIENT Manual __________________________________________________________ 15
Note: The minimum temperature you can set is –3°C.
4.12 Save program
Save program with [C pgm OK]. The program data are stored in the permanent memory.
Directory:
3
program no.
0
number of steps: 5
runtime:
1h10m23s
edit
A
B
Name:
C
TEST 1
D
The runtime is calculated and displayed.
Note: To view the current remaining runtime at any time of the run press [A info] in the main
menu.
TGRADIENT Manual __________________________________________________________ 16
5
View/edit program data
You can check any existing program by scrolling through the editing mode with cursor keys
ïðñò. Select the desired program as described in section 4.2.
Note: During operation of the cycler the active program can be viewed but not modified. If you
want to change settings of the active program for further experiments you have to save a copy of
this program to another memory. For further information about copying programs see section
5.2.
5.1 Delete / insert program steps
The programming spreadsheet:
Temp[°C]
time
ß #
2: 95.0 0h 0m30s
3: 55.0 0h 0m30s
4: 72.0 0h 0m30s 2
29
5:
A ?
B insert/delete
gradient[°C]
C pgm OK
opt à
D enter
To delete or insert a program step press [B insert/delete]
insert /
2: 95.0
3: 55.0
4: 72.0
5:
A ?
delete step:
0h 0m30s
0h 0m30s
0h 0m30s 2
29
B delete input
C quit
D enter
Enter the number of the step that you want to insert / delete.
Note: Additional steps will be inserted before the selected step number. The following steps will
be shifted by one position.
insert /
2: 95.0
3: 55.0
4: 72.0
5:
A ?
delete step:
0h 0m30s
0h 0m30s
0h 0m30s 2
3
29
B delete input
C quit
D enter
Note: If you have entered a wrong number, the field can be cleared with [B delete input].
Confirm the step number with [D enter].
TGRADIENT Manual __________________________________________________________ 17
insert / delete step:
2: 95.0 0h 0m30s
3: 55.0 0h 0m30s
4: 72.0 0h 0m30s 2
5:
A insert
B delete
3
29
C quit
D enter
Press [A insert] to insert one step at the selected position.
Note: Additional steps will be inserted before the selected step. The following steps will be
shifted by one position.
Press [B delete] to delete the selected step.
Note: By deleting a step the following steps will be shifted by one position.
Note: The insertion and deletion of steps may have an influence on existing cycles. Be sure that
all settings are updated to maintain a correct cycle.
5.2 Copy program
Select the program you want to copy as described in section 4.1 and 4.2
Directory:
program no.
lid temp:
A ?
3
0
105°C
Name:
TEST 1
preheating: on
B files
C pgm OK
D enter
Press [B files].
Directory:
program no.
lid temp:
edit
A copy
3
0
°C
Name:
TEST 1
preheating: on
B delete pgm
C quit
D enter
To copy this program to another memory press [A copy]
copy to:
Main direct.--A ?
½-3.
½-4.
½-5.
½-6.
½-7.
B
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
D enter
Select the desired subdirectory with the cursor keys ðñ ò. The selected subdirectory will
highlight.
TGRADIENT Manual __________________________________________________________ 18
½-3.
½-4.
½-5.
½-6.
½-7.
copy to:
Main direct.--A ?
B
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
D enter
Confirm your selection with [D enter].
copy from directory 3 program 0
to
directory 6 program
A list
B
C quit
D enter
Choose an empty memory from the list with [A list].
copy from directory 3 program 0
to
directory 6 program 6
enter = OK
target program contains
A list
B
0 steps
C quit
D enter
Note: Before you confirm with [D enter] the target memory is checked. If there is no program in
the target, memory the message “ target program contains 0 steps” is displayed. If the memory
already contains a program the number of steps is displayed.
Note: If you copy a program to a memory that already contains a program, the former program
will be deleted!
Save a copy of the program in the new directory with [D enter].
5.3 Delete program
Select program as described in section 4.1 and 4.2
Directory:
program no.
lid temp:
edit
A copy
3
0
°C
Name:
TEST 1
preheating: on
B delete pgm
To delete this program press [B delete pgm].
C quit
D enter
TGRADIENT Manual __________________________________________________________ 19
Directory:
program no.
lid temp:
3
0
°C
Name:
TEST 1
preheating: on
delete program 0?
A copy
B delete pgm
enter = yes
C quit
Confirm your selection with [D enter].
Note: Once you have deleted a program it cannot be restored!
D enter
TGRADIENT Manual __________________________________________________________ 20
6
Further programming options
Temp[°C]
1: 95.0
2: 95.0
3: 55.0
4: 72.0
A ?
time
ß
0h 5m 0s
0h 0m30s
0h 0m30s
0h 0m30s
2
B header
#
gradient[°C]
C pgm OK
opt à
D enter
Note: To program special parameters you can scroll the display to the right.
Move the cursor to the right side of the display that is labeled with opt à.
The following screen will appear:
Main funct.
dT[°C]
1: ß
2: ß
3: ß
4: ß
A ?
B header
dt[s]
C pgm OK
ä[°/s]
5.00
5.00
5.00
5.00
D enter
Note: To get back to the screen with the basic parameters move the cursor to the left side of the
display labeled with “Main funct.” Alternatively you can move the cursor to the extreme right.
Temp[°C]
1: 95.0
2: 95.0
3: 55.0
4: 72.0
A ?
time
ß
0h 5m 0s
0h 0m30s
0h 0m30s
0h 0m30s
2
B header
#
gradient[°C]
opt à
+
C pgm OK
D enter
Note: In the main screen you will find an information that a special parameter has been
modified. This is indicated by a + in the row labeled with opt à.
6.1 Program time increments
To compensate for loss in enzyme activity each step within a loop can be extended from cycle to
cycle. Enter the desired time increment [seconds] in the row labeled with dt[s]. This value will
be added to the time value from cycle to cycle.
Main funct.
dT[°C]
2: ß
3: ß
4: ß
5: ß
A ?
B header
dt[s]
10
C pgm OK
ä[°/s]
5.00
5.00
5.00
5.00
D enter
TGRADIENT Manual __________________________________________________________ 21
Note: The time increment will have an impact on the total runtime depending on the numbers of
cycles and the size of the increment. A program with many cycles and large time increments will
take a significantly longer time than a standard protocol.
6.2 Program touch down
For some applications it is useful to start with a higher annealing temperature and to decrease the
temperature from cycle to cycle. This subsequent lowering of the annealing temperature is called
touch down.
To decrease the annealing temperature from cycle to cycle enter a negative temperature
increment in the row labeled with dT[s].
Main funct.
dT[°C]
2: ß
3: ß
4: ß
-0.20
5: ß
A ?
B header
dt[s]
C pgm OK
ä[°/s]
5.00
5.00
5.00
5.00
D enter
Note: Be sure that the temperature decrease is set in a step that lies within a loop. Otherwise
there will be no iterative temperature decrease.
6.3 Adjust heating and cooling ramps
Since the TGRADIENT is a extreme fast thermocycler it may be necessary to reduce the heating
and cooling ramp for some applications. For example this is helpful if cycling protocols from
other cyclers shall be used.
The heating or cooling ramp respectively can be set in the row labeled with ä[°/s].
Main funct.
dT[°C]
2: ß
3: ß
4: ß
5: ß
A ?
B header
dt[s]
C pgm OK
ä[°/s]
5.00
5.00
5.00
5.00
D enter
Note: The default is [5.00 °/s] which means maximum ramping speed.
TGRADIENT Manual __________________________________________________________ 22
7
Run Program
7.1 Select and start program
Main display:
Prog
step
temp[°C]
time
lid
A ?
25.2°C
25.4°C
B start/stop
C programs
D +
To select a program press [B start/stop]
start
Main direct.--A ?
½-3.
½-4.
½-5.
½-6.
½-7.
B
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
D enter
Choose directory with cursor keys ð ñ ò or enter main directory with [D enter]
Note: The path for the program that was modified most recently is automatically pre-selected
Main direct.---
A ?
½-3.
½-4.
½-5.
subdirect.:
subdirect.:
Subdirect.:
½-6.
½-7.
subdirect.:
subdirect.:
C quit
B
D enter
Enter subdirectory with [D enter]
Directory: 5
program no.
Edit
A list
B
Name:
C quit
D enter
Enter the number of the program you want to start. Alternatively, [press A] list to select a
program from the list of all programs in this subdirectory.
Scroll through the list with cursor keys ñ ò.
TGRADIENT Manual __________________________________________________________ 23
0
1
2
3
4
A
test 1
empty
empty
empty
empty
list
B forward
C quit
D enter
Confirm highlighted program with [D enter].
The number and name of both subdirectory and the selected program will be displayed.
Directory:
program no.
3
0
Name:
TEST 1
preheating lid on
signal on
C quit
D start
start block
A list
B
To start the program press [D start].
7.2 Display during operation
During preheating of the lid the following screen is displayed:
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
25.0°C
preheating
B start/stop
dir 3
à 95.0
C programs
D +
Note: In the default setting (preheating on) the block is held constantly at 25°C. The program
starts as soon as the lid has reached the set temperature. During the preheating phase the display
of the lid temperature alternates between the current lid temperature and “preheating”.
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
25.0°C
dir 3
à 95.0
preheating
B start/stop C programs
D +
As soon as the block has reached the programmed hold temperature, the timer starts to count
down.
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
95.0°C
0h 2m15s
104.9°C
B start/stop
dir 3
cycle 1 of 30
à 95.0
à0h 5m 0s
C programs
D +
TGRADIENT Manual __________________________________________________________ 24
The elapsed time versus programmed hold time is displayed
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
dir 3
1
cycle 1 of 30
49.9 55.0 60.0 à 50.0 55.0 60.0
0h 0m23s
à0h 5m 0s
104.9°C
B start/stop
C programs
D +
As soon as the block enters a gradient step the three temperatures of the left, middle and right
sides of the block are displayed.
7.3 View remaining run time
Display during operation:
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
dir 3
1
cycle 1 of 30
49.9 55.0 60.0 à 50.0 55.0 60.0
0h 0m23s
à0h 5m 0s
104.9°C
B start/stop
C programs
D +
Press [A info] to call up the current remaining time calculation.
Additional information about active pgm
Status:
Step 2
Cycle 3 of 30
Remaining time: 2h 57m
Stop or pause: key B in main menu
A gradient
B
C quit
D
The current remaining run time id displayed in line 4.
7.4 View current gradient temperatures
While the TGRADIENT is cycling you can view the current gradient temperatures. To retrieve
detailed information about the temperatures in the different rows press [A info]
in the main screen.
Additional information about active pgm
Status:
Step 2
Cycle 3 of 30
Remaining time: 2h 57m
Stop or pause: key B in main menu
A gradient
B
C quit
D
Then press [A gradient]
TGRADIENT Manual __________________________________________________________ 25
Temperatures of current gradient
T 1- 6: 49.4 49.6 50.3 51.4 52.6 53.8 °C
T 7-12: 55.0 56.2 57.3 58.4 59.1 59.4 °C
remaining time: 2h56m
A gradient
B
C quit
D
Note: The temperatures can be displayed online during each gradient step (see section 7.4). As
the program moves to the next step the temperature data of the last gradient are stored in the
temporary memory. Thus you can check the temperatures of the last gradient in consecutive
steps.
Note: If there has not been yet a gradient step in the protocol yet, you will get the message: “No
gradient data available”.
To leave the window press [C quit]
7.5 Pause / stop program
Main display during operation:
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
72.0°C
0h 0m18s
104.8°C
B start/stop
dir 3
cycle 8 of 30
à 72.0
à0h 0m30s
C programs
D +
To pause or stop the active program press [B start/stop]
Stop/pause
directory:
program no:
A ?
Select
[B pause]
[D stop]
[C quit ]
3
0 test 1
B pause
C quit
D stop
to pause program
to stop program
to return to the program display
When you select [B pause], the following screen is displayed:
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
72.0°C
104.8°C
B start/stop
dir 3
cycle 8 of 30
à 72.0
pause
C programs
D +
The program pauses in the current state. The word pause is displayed and alternates with the
elapsed time. You can proceed with the program with [B start/stop].
TGRADIENT Manual __________________________________________________________ 26
8
Special functions
Main screen
Prog
step
temp[°C]
time
lid
A ?
25.2°C
25.4°C
B start/stop
C programs
D +
To change special features of the TGRADIENT press [D +].
1
2
3
4
5
print
signal
language
standard mode
test mode
A back
B forward
C quit
D enter
Scroll though the list with the cursor keys ñ ò. Enter desired function with [D enter]
8.1
Print protocols
1 graphic protocol*
2 graphic protocols*
3 print program
4 print all programs
A
B
C quit
D enter
Scroll through the list with the cursor keys ñ ò. Confirm the highlighted function with [D
enter].
8.2 Switch beep on/off
There is an option for an audible signal whenever a program enters a pause and when the
program is finished. If this option is active, the beep can be switched off by pressing any key of
the keyboard.
with signal
A
yes
no
B
C quit
D enter
In this window you can select whether there is a signal or whether there is not. Using the cursor
keys you can toggle between signal yes/no. Confirm your selection with [D enter].
TGRADIENT Manual __________________________________________________________ 27
8.3 Select language
The display of the TGRADIENT can be switched from English to German. Select language with
cursor keys ò ñ. As you toggle between the two settings the language will change.
language
A
German
English
B
C quit
D enter
To confirm your selection press [D enter]
8.4 RS232 Control
The Tgradient can be programmed and controlled by Personal Computer. The Tcontrol iT
software is available with the order number 050-820. To acivate remote control, the Tgradient
has to be set to RS232 control mode.
For detailled information on controlling Tgradient with PC please refer to the Tcontrol iT
manual.
8.5 Block type
Display and calculation of gradient temperatures depends on the block version used. A
temperature gradient can be run with either 48 well and 96 well modules. After changing block
modules from one type to the other, the block type must be set in the instruments software.
Go to special function 8 [block type] and set correct block version.
TGRADIENT Manual __________________________________________________________ 28
9
Maintenance
The TGRADIENT was built to operate for a long time without the need for periodical
maintenance. The housing may be cleaned from time to time with a smooth cotton cloth. Do not
use strong detergents or organic solvents for cleaning.
10 Exchange of modules
For exchange of modules you need two hexagonal keys (number 3 and number 5). These keys
are included in the shipment of the module.
Remove module
Ü
Ü
Ü
Ü
Ü
Ü
Remove power plug
Place instrument upside down on a soft surface
Remove the 2 screws that close the shutter (hexagonal key number 3)
Remove the 4 screws that hold the block (hexagonal key number 4)
unplug connectors from the block (control and power supply)
remove module
Install module
Follow description in reversed order.
TGRADIENT Manual __________________________________________________________ 29
11 Trouble shooting
11.1 Slow heating and cooling
The T1 THERMOCYCLER is equipped with a strong ventilator for the cooling of the heat sink. The
inlet of this fan is located at the bottom side of the instrument. Be sure that the inlet is not
clogged by dust or other material (e.g. a sheet of paper placed under the cycler can be attached to
the inlet as the fan is in operation). Dirt can be removed easily from the inlet with a conventional
vacuum cleaner.
11.2 Adaptation of protocols from other cyclers
Since the T1 THERMOCYCLER is an extremely fast thermocycler it may be necessary to reduce
the heating and cooling ramps to run protocols from other cyclers. For the setting of the heating
and cooling ramps see section 6.3. Alternatively, the time settings may be extended.
Note: Protocolls that have been optimized with a TGradient thermocycler can be run without
changes.
TGRADIENT Manual __________________________________________________________ 30
12 Trouble shooting
12.1 Slow heating and cooling
The TGRADIENT is equipped with a strong ventilator for the cooling of the heat sink. The inlet of
this fan is located at the bottom side of the instrument. Be sure that the inlet is not clogged by
dust or other material (e.g. a sheet of paper placed under the cycler can be attached to the inlet as
the fan is in operation). Dirt can be removed easily from the inlet with a conventional vacuum
cleaner.
12.2 Restart due to unrecognized power failure
High voltage fluctuation can lead to an automatic restart of the thermocycler. In this case the
cycler restarts at the step where there power failure has occurred. To avoid voltage fluctuation,
do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a
centrifuge.
12.3 Adaptation of protocols from other cyclers
Since the TGRADIENT is an extremely fast thermocycler it may be necessary to reduce the
heating and cooling ramps to run protocols from other cyclers. For the setting of the heating and
cooling ramps see section 6.3. Alternatively, the time settings may be extended.
TGRADIENT Manual __________________________________________________________ 31
13 Appendix
13.1 Technical specification TGradient 96
Thermal range
-3°C – 100°C
Temperature accuracy
+ 0.1°C
Thermal homogeneity
+ 0.3°C within 15 sec.
Range of Gradient
40°C
Maximum heating speed
4°C/sec.
Max. cooling speed
3°C/sec.
Sample capacity
96 x 0.2ml tubes, 96 well microplates, strips
Line Voltage
115 Volt or 230 Volt
Frequency
50-60 Hz
Maximum power consumption
310 Watt
Fuses
see label on the back of the instrument
Display
LCD graphical display, back-lit
Ports
serial: RS232 (bi-directional data transfer)
parallel: Centronics (printer)
Port for in tube-sensor
memory
Total capacity: 1500 steps
in 10 directories with maximum 100 programs each
Dimensions (WxDxH)
Weight
Storage conditions
Operating conditions
25 x 34 x 16,5 cm
8,8 kg
5°C – 35°C
13.2 Ordering information
TGRADIENT Thermocycler
TGRADIENT 48 for 48 x 0.5ml tubes
TGRADIENT 96 for 96 x 0.2ml tubes, strips or 96 well microplates
Order-No.
050-800
050-801
TGRADIENT Modules
TGRADIENT 48 Module for 48 x 0.5ml tubes
TGRADIENT 96 Module for 96 x 0.2ml tubes, strips or 96 well microplates
TGRADIENT 384 Module for 384 well microplates (non gradient)
TGRADIENT in situ Module for 4 microscopic slides (non gradient)
050-810
050-811
050-812
050-813
For information about tubes, strips, microplates and other consumables please contact
BIOMETRA or your local BIOMETRA distributor.
TGRADIENT Manual __________________________________________________________ 32
14 Instructions for return shipment
In case of faults occurring with our TGRADIENT Thermocycler please proceed as follows:
· Return only defective devices. For technical problems which are not definitively recognizable
as device faults please contact the Technical Service Department at Biometra ( Tel.:
+49/551/5068640, Fax: ++49/551/5068666, e-mail: [email protected]).
· Important: Cleanse all parts of the instrument of biologically dangerous, chemical or
radioactive contaminants. If a an instrument is contaminated, Biometra will be forced to
refuse to accept the device. The sender of the repair order will be held liable for possible
losses resulting from insufficient decontamination of the device.
· Please prepare written confirmation that the device is free from biologically dangerous and
radioactive contaminants. The declaration of decontamination (see 16) has to be attached to
the outside of the packaging.
· Use the original packing material. If not available, please use a comparable robust box. Be
sure that the instrument fits tightly into the box. When packaging, protect the device in a way
to avoid
· Label the outside of the box with “CAUTION! SENSITIVE ELECTRONIC
INSTRUMENT!”
· Please enclose a note which contains the following:
a) Sender’s name and address,
b) Name of a contact person for further inquiries with telephone number,
c) Description of the fault, which also reveals during which procedures the fault occurred, if
possible
TGRADIENT Manual __________________________________________________________ 33
15 Warranty
This Biometra instrument has been carefully built, inspected and quality controlled before
dispatch. Hereby Biometra warrants that this instrument conforms to the specifications given in
this manual. This warranty covers defects in materials or workmanship for 24 months as
described under the following conditions:
This warranty is valid for 12 month from date of shipment to the customer from Biometra or an
authorized distributor. This warranty will not be extended to a third party without a written
agreement of Biometra.
This warranty covers only the instrument and all original accessories delivered with the
instrument. This warranty is valid only if the instrument is operated as described in the manual.
Biometra will repair or replace each part which is returned and found to be defective. This
warranty does not apply to wear from normal use, failure to follow operating instructions,
negligence or to parts altered or abused.
TGRADIENT Manual __________________________________________________________ 34
16 Equipment Decontamination Certificate
To enable us to comply with German law (i.e. §28 StrlSchV, §17 GefStoffV and §19 ChemG) and to avoid
exposure to hazardous materials during handling or repair, will you please complete this form, prior to the
equipment leaving your laboratory
COMPANY / INSTITUTE ____________________________________________________________
ADDRESS ____________________________________________________________
TEL NO _____________________ FAX NO _____________________ E-MAIL _____________________
______________________________
EQUIPMENT
If on loan / evaluation
Model
Serial No
__________
__________
__________
__________
__________
__________
__________
__________
Start Date: ________ Finish Date ________
Hazardous materials used with this equipment
_________________________________________________________________________________
_________________________________________________________________________________
_________________________________________________________________________________
Has the equipment been cleaned and decontaminated? YES / NO (delete)
Method of cleaning / Decontamination
_________________________________________________________________________________
_________________________________________________________________________________
_________________________________________________________________________________
NAME ________________________________ POSITION __________________________
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
SIGNED ______________________________ DATE ______________________________
PLEASE RETURN THIS FORM TO BIOMETRA GmbH OR YOUR LOCAL BIOMETRA DISTRIBUTOR
TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS WITHOUT THIS
CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
TGRADIENT Manual ____________________________________________________________I
1
2
Introduction .............................................................................................................................2
Before you start .......................................................................................................................3
2.1 Safety warnings ...................................................................................................................3
2.2 Warning symbols.................................................................................................................3
3
First steps with the TGRADIENT ..............................................................................................4
3.1 The TGRADIENT Thermocycler front View.........................................................................4
3.2 The TGRADIENT rear view ..................................................................................................5
3.3 The TGradient control panel ...............................................................................................6
3.4 Initial self test ......................................................................................................................7
3.5 The TGradient display.........................................................................................................7
3.6 Handling of the adjustable heated Lid.................................................................................7
3.7 Releasing wheel in case of blocked lid ...............................................................................8
4
Creating a Program..................................................................................................................9
4.1 Select a directory.................................................................................................................9
4.2 Select a program store .........................................................................................................9
4.3 Enter name for subdirectory..............................................................................................10
4.4 Enter a program name .......................................................................................................10
4.5 Enter the lid temperature ...................................................................................................11
4.6 Select / deselect pre-heating of the lid ..............................................................................11
4.7 Enter temperature and time settings ..................................................................................12
4.8 Set temperature gradient....................................................................................................13
4.9 View different temperatures of the gradient in the block..................................................13
4.10 Set cycle number ...........................................................................................................14
4.11 Cool below ambient temperature ..................................................................................14
4.12 Save program.................................................................................................................15
5
View/edit program data.........................................................................................................16
5.1 Delete / insert program steps .............................................................................................16
5.2 Copy program....................................................................................................................17
5.3 Delete program ..................................................................................................................18
6
Further programming options................................................................................................20
6.1 Program time increments ..................................................................................................20
6.2 Program touch down .........................................................................................................21
6.3 Adjust heating and cooling ramps .....................................................................................21
7
Run Program .........................................................................................................................22
7.1 Select and start program ....................................................................................................22
7.2 Display during operation...................................................................................................23
7.3 View remaining run time...................................................................................................24
7.4 View current gradient temperatures ..................................................................................24
7.5 Pause / stop program .........................................................................................................25
8
Special functions ...................................................................................................................26
8.1 Print protocols ...................................................................................................................26
8.2 Switch beep on/off ............................................................................................................26
8.3 Select language..................................................................................................................27
8.4 RS232 Control...................................................................................................................27
8.5 Block type .........................................................................................................................27
9
Maintenance ..........................................................................................................................28
10 Exchange of modules ............................................................................................................28
11 Trouble shooting ...................................................................................................................29
11.1 Slow heating and cooling ..............................................................................................29
11.2 Adaptation of protocols from other cyclers...................................................................29
TGRADIENT Manual ___________________________________________________________ II
12
Trouble shooting ...................................................................................................................30
12.1 Slow heating and cooling ..............................................................................................30
12.2 Restart due to unrecognized power failure....................................................................30
12.3 Adaptation of protocols from other cyclers...................................................................30
13 Appendix ...............................................................................................................................31
13.1 Technical specification TGradient 96 ...........................................................................31
13.2 Ordering information.....................................................................................................31
14 Instructions for return shipment ............................................................................................32
15 Warranty................................................................................................................................33
16 Equipment Decontamination Certificate...............................................................................34
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