T1 Thermal Cycler Manual

T1 Thermal Cycler Manual
T1 THERMOCYCLER Manual _____________________________________________________ 1
Manual
Software Version 4.01
Sept 1999
Order-No.
050-900 T1 48
050-901 T1 96
050-902 T1 384
050-903 T1 in situ
Biometra
biomedizinische Analytik GmbH
Rudolf-Wissell-Str. 30, D-37079 Goettingen
Tel.: 0551/50 686-0; Fax: 0551/50 686-66
email: [email protected]
http://www.biometra.com
T1 THERMOCYCLER Manual _____________________________________________________ 2
1
Introduction
The T1 Thermocycler was designed to meet the highest standard of a today molecular biology
laboratory. The instrument combines the very successful Biometra silver block concept with an
advanced block architecture. The result is a thermocycler that provides extremely high
temperature ramping rates and an unrivalled temperature uniformity. High ramping rates ensure
specific reation and the perfect temperature uniformity guarantees identical conditions in any
position of the block.
The T1 Thermocyler is available with different modules that support a broad range of tubes,
strips and microplates. There is also an in situ-modul for up to 4 microscopic slides.
The new module architecture together with a high precision heated lid enables heat sealing of
microplates within the instrument. There is no need for additional hardware. The BIOMETRA
smart lid technology ensures a perfect fitting of tubes in the block, no matter what plasticware is
used.
The T1 thermocyler is adjusted to match the BIOMETRA TGradient cycling characteristics.
Thus protocols, that have been optimized with an Tgradient, can be operated without the need for
modifications. This makes the T1 the perfect instrument to increase sample throughput togehter
with an existing Tgradient.
T1 THERMOCYCLER Manual _____________________________________________________ 3
2
2.1
Before you start
Safety warnings
Before connecting the unit to the power source please ensure that the
voltage selector at the back side of the instrument is set to the required
voltage.
Danger of electric shock! Unplug the power cable before you open the T1
THERMOCYCLER.
It is not necessary to utilize oil into the opening of the block in order to
improve the heat transfer between the block and the sample tubes. If you
still decide to use oil, do not use silicon oil. Mineral oil may be used.
2.2
Warning symbols
The thermoblock and the heated lid will reach high temperatures
during operation. Both thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil
explosively. Always wear safety goggles during operation. Close the
lid before starting a program.
Ensure that both the rear and bottom ventilation slits of the rear and
bottom of the instrument are unobstructed. Insufficient ventilation
can cause overheating of the instrument.
T1 THERMOCYCLER Manual _____________________________________________________ 4
3
3.1
First steps with the T1 THERMOCYCLER
The T1 THERMOCYCLER front View
Knob for release
of the lid
Wheel for the height
adjustement
of the heated lid.
Keypad
Graphical display
Power switch
T1 THERMOCYCLER Manual _____________________________________________________ 5
3.2
The T1 THERMOCYCLER rear view
Parallel
printer port
RS-232
port
Fuse holder
for 2 fuses,
for specifications of fuses
see type label on the
instrument´s backside
Power cord
plug
Type label including:
serial number
specifications of fuses
T1 THERMOCYCLER Manual _____________________________________________________ 6
3.3
The T1 Thermocycler control panel
Graphical display
Power
Power switch:
backlit = power on
A
B
C
D
Function keys:
the corresponding functions are
specified in the screen.
7
8
9
4
5
6
1
2
3
.
0
-
Cursor keys:
For free navigation in the
programming spreadsheet.
Use to scroll through lists.
Easy to clean
keyboard
T1 THERMOCYCLER Manual _____________________________________________________ 7
3.4 Initial self test
After switching on the T1 THERMOCYCLER the serial number of the instrument and the software
version is displayed.
T1 Thermocycler
Serial No 1234567
Vers. 4.01
The T1 THERMOCYCLER checks the programs in all directories (so called RAM check).
3.5 The T1 Thermocycler display
The T1 THERMOCYCLER display provides information about the instrument and about the status
of the active program.
prog
step
temp[°C]
time
lid
A ?
25.2°C
25.4°C
B start/stop
C programs
D +
The individual function of the special keys A , B , C and D are specified in the display and
depend on the context.
3.6 Handling of the adjustable heated Lid
To achieve optimum pressure on the tubes the T1 THERMOCYCLER is equipped with an height
adjustable heated lid (smart lid technology).
Close the lid:
After the samples have been placed in the block close the lid. Turn the wheel clockwise until you
hear a clicking noise. In this mode, the pressure will not increase further, even when you keep on
turning the wheel.
Note: The pressure of the lid has been optimize for a fully loaded block. If only very few tubes
are loaded to the block you should place dummy tubes in the four corner positions to avoid
damage of tubes by excessive pressure.
Open the heated lid:
First: Release pressure by turning the wheel counter clockwise. As soon as there is no more
resistance the pressure has been released. Then: now you can open the lid with the knob.
Important: The lid must not be opened under pressure because this leads to damage of the
locking mechanism.
T1 THERMOCYCLER Manual _____________________________________________________ 8
4
Creating a Program
4.1 Select a directory
In the T1 THERMOCYCLER programs can be stored both in the main directory and in individual
subdirectories. For easy identification of the selected storage place, the subdirectories can be
named (see section 4.3).
Start with the main screen.
Prog
step
temp[°C]
time
lid
A ?
25.2°C
25.4°C
B start/stop
C programs
D +
Press [C programs] to enter the edit mode.
-3.
-4.
Main direct.--- -5.
-6.
-7.
A ?
B
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
D enter
You are now in the main directory. The structure of the subdirectories is displayed.
4.2 Select a program store
Programs can be stored in the main directory or in subdirectories.
To create a program in the main directory press [D enter].
To access a subdirectory move right with the cursor key and select a subdirectory with the
cursor keys
. The selected directory will be highlighted.
-3.
-4.
Main direct.--- -5.
-6.
-7.
A ?
B
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
D enter
T1 THERMOCYCLER Manual _____________________________________________________ 9
4.3 Enter name for subdirectory
The process for naming a subdirectory and a program is the same. For information about naming
programs see section 4.4.
Press [D enter] to enter the highlighted subdirectory
directory:
program no.
edit
A list
5
Name:
B
C quit
D enter
Press [A list] to view a list of all existing files and empty memory in this directory.
Note: If neither program name nor the word “empty” is displayed, this place may be occupied by
a program without name. For the programming of new protocols choose an empty memory.
Existing programs that are not needed any more can be deleted to provide new memory.
0
1
2
3
4
A
empty
empty
empty
empty
empty
list
B forward
Scroll through the list with cursor keys
C quit
D enter
and confirm memory with [D enter].
4.4 Enter a program name
Each program is specified by a program number and a subdirectory number. To make retrieval of
a program easier, you can enter a name for each program existing of letters, numbers and
symbols.
Directory:
program no.
A ABC
3
0
B files
Name:
∧
ABCDEFGHIJKLMNOPQRST
UVWXYZ-()#°C/,〈〉&+.%!
C name OK
D enter
To enter a name for your program press [A ABC].
Directory:
program no.
A name
3
0
B blank
Name:
∧
ABCDEFGHIJKLMNOPQRST
UVWXYZ-()#°C/,〈〉&+.%!
C quit
D enter
T1 THERMOCYCLER Manual ____________________________________________________ 10
Choose a letter from the alphabet with cursor keys
Confirm each letter with [D enter].
Directory:
program no.
A name
3
0
B blank
Name:
T
∧
ABCDEFGHIJKLMNOPQRST
UVWXYZ-()#°C/,〈〉&+.%!
C name OK
D enter
The selected letter will appear in the display and the cursor will move one step to the right.
Select the next letter and confirm with [D enter].
Note: What to do, if a wrong letter has been accepted?
To alter the position of the cursor within the name press [A name]. Now you can move the cursor
with cursor keys
through the name. This is helpful if you have confirmed an wrong letter or
if you want to change the name of an existing program.
Once the name is complete, confirm name with [C name OK].
Directory:
program no.
lid temp:
edit
A ?
3
0
°C
B files
Name:
TEST 1
preheating: on
C pgm OK
D enter
4.5 Enter the lid temperature
You can now enter a temperature for the heated lid. Note: In general, the lid temperature should
be about 10°C above the highest temperature in the protocol. Example: If your denaturation step
is at 95°C, enter 105°C for the lid.
Directory:
3
program no.
0
lid temp:
105 °C
edit
A ?
B files
Name:
TEST 1
preheating: on
C pgm OK
D enter
Note: For heat sealing of microplates set the lid temperature to 110 °C (see section 9)
4.6 Select / deselect pre-heating of the lid
You can choose whether the lid is pre-heated before the program starts. This is to avoid
condensation during the initial heating phase. Note: During the preheating of the lid, the block is
held constant at 25°C.
T1 THERMOCYCLER Manual ____________________________________________________ 11
Directory:
3
program no.
0
lid temp:
105 °C
edit
A on/off
Name:
TEST 1
preheating: on
B files
C pgm OK
D enter
Note: The default setting, which is recommended for most applications, is preheating: on.
After all pre-settings have been made, press [D enter] to open the programming spreadsheet.
4.7 Enter temperature and time settings
The programming spreadsheet:
Temp[°C]
1
2:
3:
4:
A ?
time
#
B insert/delete
opt
C pgm OK
D enter
Note: In this spreadsheet you can enter all parameters for your cycling protocol. Furthermore,
you can set special parameters like touch down, ramping rates or time increments. For further
details on these special parameters, see section 6.
Note: You can navigate in the spreadsheet without limitations with the four cursor keys
.
Note: Each setting is confirmed with [D enter]. The cursor moves automatically to the next field.
Alternatively, you can confirm a value by moving forward with the cursor keys.
Note: At any time you can call up the help function with [A ?]
Note: In an existing protocol hole program steps can be deleted and inserted. For further
information on deleting and inserting program steps see section 5.1.
Now enter temperature for the first step in the protocol:
Temp[°C]
time
#
1
95,0
2:
3:
4:
A ?
B insert/delete
opt
C pgm OK
D enter
Confirm temperature with [D enter] or just move on with the cursor key. If you move to the next
row, the setting will be stored in the temporary memory.
T1 THERMOCYCLER Manual ____________________________________________________ 12
Temp[°C]
1
95.0
2:
3:
4:
A ?
time
#
B insert/delete
opt
C pgm OK
D enter
Enter a time for step one.
Note: There is a convention on how time settings are entered in all BIOMETRA cyclers:
hours minutes seconds
If you enter a number without “dot” this value will be interpreted as seconds (“300” => 5
minutes). To program minutes enter a “ ” after the number of minutes. To enter hours enter
after the number. You can also enter any combination of hours, minutes and seconds. Example:
for 1 hour, 30 minutes, 20 seconds enter 1 30 20.
The time values will be displayed in the following format: 0h 00 m 00s
Accept time setting with [D enter] or move to the next field with cursor key.
4.8 Set cycle number
Note: In general, loops are defined by selecting the target for the back loop and the number of
backloops.
Temp[°C]
1: 95.0
2: 95.0
3: 55.0
4: 72.0
A ?
time
#
0h 5m 0s
0h 0m30s
0h 0m30s
0h 0m30s
2
B insert/delete
In the column labeled with
Temp[°C]
1: 95.0
2: 95.0
3: 55.0
4: 72.0
A ?
opt
10°C
C pgm OK
D enter
you can select the target step for a backloop.
time
#
0h 5m 0s
0h 0m30s
0h 0m30s
10°C
0h 0m30s 2
29
B insert/delete
C pgm OK
opt
D enter
Enter the number of backloops. Note: total cycler number = (n backloops) + 1, e.g. enter 29 for a
total number of 30 cycles.
4.9
Cool below ambient temperature
T1 THERMOCYCLER Manual ____________________________________________________ 13
Temp[°C]
2: 95.0
3: 55.0
4: 72.0
5: 4.0
A ?
time
#
0h 0m30s
0h 0m30s
10°C
0h 0m30s 2
29
pause
B insert/delete
C pgm OK
opt
D enter
To hold the temperature for an indefinite time enter “0”. After pressing [D enter] “pause” is
displayed.
Note: The minimum temperature you can set is –3°C.
4.10 Save program
Save program with [C pgm OK]. The program data are stored in the permanent memory.
Directory:
3
program no.
0
number of steps: 5
runtime:
1h10m23s
edit
A
B
Name:
C
TEST 1
D
The runtime is calculated and displayed.
Note: To view the current remaining runtime at any time of the run press [A info] in the main
menu.
T1 THERMOCYCLER Manual ____________________________________________________ 14
5
View/edit program data
You can check any existing program by scrolling through the editing mode with cursor keys
. Select the desired program as described in section 4.2.
Note: During operation of the cycler the active program can be viewed but not modified. If you
want to change settings of the active program for further experiments you have to save a copy of
this program to another memory. For further information about copying programs see section
5.2.
5.1 Delete / insert program steps
The programming spreadsheet:
Temp[°C]
time
#
2: 95.0 0h 0m30s
3: 55.0 0h 0m30s
4: 72.0 0h 0m30s 2
29
5:
A ?
B insert/delete
opt
10°C
C pgm OK
D enter
To delete or insert a program step press [B insert/delete]
insert /
2: 95.0
3: 55.0
4: 72.0
5:
A ?
delete step:
0h 0m30s
0h 0m30s
0h 0m30s 2
10°C
29
B delete input
C quit
D enter
Enter the number of the step that you want to insert / delete.
Note: Additional steps will be inserted before the selected step number. The following steps will
be shifted by one position.
insert /
2: 95.0
3: 55.0
4: 72.0
5:
A ?
delete step:
0h 0m30s
0h 0m30s
0h 0m30s 2
3
10°C
29
B delete input
C quit
D enter
Note: If you have entered a wrong number, the field can be cleared with [B delete input].
CONFIRM THE STEP NUMBER WITH [D ENTER].
T1 THERMOCYCLER Manual ____________________________________________________ 15
insert / delete step:
2: 95.0 0h 0m30s
3: 55.0 0h 0m30s
4: 72.0 0h 0m30s 2
5:
A insert
B delete
3
10°C
29
C quit
D enter
Press [A insert] to insert one step at the selected position.
Note: Additional steps will be inserted before the selected step. The following steps will be
shifted by one position.
Press [B delete] to delete the selected step.
Note: By deleting a step the following steps will be shifted by one position.
Note: The insertion and deletion of steps may have an influence on existing cycles. Be sure that
all settings are updated to maintain a correct cycle.
5.2 Copy program
Select the program you want to copy as described in section 4.1 and 4.2
Directory:
program no.
lid temp:
A ?
3
0
105°C
Name:
TEST 1
preheating: on
B files
C pgm OK
D enter
Press [B files].
Directory:
program no.
lid temp:
edit
A copy
3
0
°C
Name:
TEST 1
preheating: on
B delete pgm
C quit
D enter
To copy this program to another memory press [A copy]
-3.
-4.
Main direct.--- -5.
-6.
-7.
copy to:
A ?
B
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
Select the desired subdirectory with the cursor keys
highlight.
D enter
. The selected subdirectory will
T1 THERMOCYCLER Manual ____________________________________________________ 16
-3.
-4.
Main direct.--- -5.
-6.
-7.
copy to:
A ?
B
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
D enter
Confirm your selection with [D enter].
copy from directory 3 program 0
to
directory 6 program
A list
B
C quit
D enter
Choose an empty memory from the list with [A list].
copy from directory 3 program 0
to
directory 6 program 6
enter = OK
target program contains
A list
B
0 steps
C quit
D enter
Note: Before you confirm with [D enter] the target memory is checked. If there is no program in
the target, memory the message “ target program contains 0 steps” is displayed. If the memory
already contains a program the number of steps is displayed.
Note: If you copy a program to a memory that already contains a program, the former program
will be deleted!
Save a copy of the program in the new directory with [D enter].
5.3 Delete program
Select program as described in section 4.1 and 4.2
Directory:
program no.
lid temp:
edit
A copy
3
0
°C
Name:
TEST 1
preheating: on
B delete pgm
To delete this program press [B delete pgm].
C quit
D enter
T1 THERMOCYCLER Manual ____________________________________________________ 17
Directory:
program no.
lid temp:
3
0
°C
Name:
TEST 1
preheating: on
delete program 0?
A copy
B delete pgm
enter = yes
C quit
Confirm your selection with [D enter].
Note: Once you have deleted a program it cannot be restored!
D enter
T1 THERMOCYCLER Manual ____________________________________________________ 18
6
Further programming options
Temp[°C]
1: 95.0
2: 95.0
3: 55.0
4: 72.0
A ?
time
0h 5m 0s
0h 0m30s
0h 0m30s
0h 0m30s
2
B header
#
opt
10°C
C pgm OK
D enter
Note: To program special parameters you can scroll the display to the right.
Move the cursor to the right side of the display that is labeled with opt .
The following screen will appear:
Main funct.
dT[°C]
1:
2:
3:
4:
A ?
B header
dt[s]
C pgm OK
[°/s]
5.00
5.00
5.00
5.00
D enter
Note: To get back to the screen with the basic parameters move the cursor to the left side of the
display labeled with “Main funct.” Alternatively you can move the cursor to the extreme right.
Temp[°C]
1: 95.0
2: 95.0
3: 55.0
4: 72.0
A ?
time
0h 5m 0s
0h 0m30s
0h 0m30s
0h 0m30s
2
B header
#
opt
10°C
C pgm OK
+
D enter
Note: In the main screen you will find an information that a special parameter has been
modified. This is indicated by a + in the row labeled with opt .
6.1 Program time increments
To compensate for loss in enzyme activity each step within a loop can be extended from cycle to
cycle. Enter the desired time increment [seconds] in the row labeled with dt[s]. This value will
be added to the time value from cycle to cycle.
Main funct.
dT[°C]
2:
3:
4:
5:
A ?
B header
dt[s]
10
C pgm OK
[°/s]
5.00
5.00
5.00
5.00
D enter
T1 THERMOCYCLER Manual ____________________________________________________ 19
Note: The time increment will have an impact on the total runtime depending on the numbers of
cycles and the size of the increment. A program with many cycles and large time increments will
take a significantly longer time than a standard protocol.
6.2 Program touch down
For some applications it is useful to start with a higher annealing temperature and to decrease the
temperature from cycle to cycle. This subsequent lowering of the annealing temperature is called
touch down..
To decrease the annealing temperature from cycle to cycle enter a negative temperature
increment in the row labeled with dT[s].
Main funct.
dT[°C]
2:
3:
4:
-0.20
5:
A ?
B header
dt[s]
C pgm OK
[°/s]
5.00
5.00
5.00
5.00
D enter
Note: Be sure that the temperature decrease is set in a step that lies within a loop. Otherwise
there will be no iterative temperature decrease.
6.3 Adjust heating and cooling ramps
Since the T1 THERMOCYCLER is a extremely fast thermocycler it may be necessary to reduce the
heating and cooling ramp for some applications. For example this is helpful if cycling protocols
from other cyclers shall be used.
The heating or cooling ramp respectively can be set in the row labeled with
Main funct.
dT[°C]
2:
3:
4:
5:
A ?
B header
dt[s]
C pgm OK
[°/s].
[°/s]
5.00
5.00
5.00
5.00
D enter
Note: The default is [5.00 °/s], which means maximum ramping speed.
T1 THERMOCYCLER Manual ____________________________________________________ 20
7
Run Program
7.1 Select and start program
Main display:
Prog
step
temp[°C]
time
lid
A ?
25.2°C
25.4°C
B start/stop
C programs
D +
To select a program press [B start/stop]
-3.
-4.
Main direct.--- -5.
-6.
-7.
start
A ?
B
Choose directory with cursor keys
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
D enter
or enter main directory with [D enter]
Note: The path for the program that was modified most recently is automatically pre-selected
-3.
-4.
Main direct.--- -5.
-6.
-7.
A ?
B
subdirect.:
subdirect.:
Subdirect.:
subdirect.:
subdirect.:
C quit
D enter
Enter subdirectory with [D enter]
Directory: 5
program no.
Edit
A list
Name:
B
C quit
D enter
Enter the number of the program you want to start. Alternatively, [press A] list to select a
program from the list of all programs in this subdirectory.
Scroll through the list with cursor keys
.
T1 THERMOCYCLER Manual ____________________________________________________ 21
0
1
2
3
4
A
test 1
empty
empty
empty
empty
list
B forward
C quit
D enter
Confirm highlighted program with [D enter].
The number and name of both subdirectory and the selected program will be displayed.
Directory:
program no.
3
0
Name:
TEST 1
preheating lid on
signal on
C quit
D start
start block
A list
B
To start the program press [D start].
7.2 Display during operation
During preheating of the lid the following screen is displayed:
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
25.0°C
preheating
B start/stop
dir 3
95.0
C programs
D +
Note: In the default setting (preheating on) the block is held constantly at 25°C. The program
starts as soon as the lid has reached the set temperature. During the preheating phase the display
of the lid temperature alternates between the current lid temperature and “preheating”.
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
25.0°C
dir 3
95.0
preheating
B start/stop C programs
D +
As soon as the block has reached the programmed hold temperature, the timer starts to count
down.
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
95.0°C
0h 2m15s
104.9°C
B start/stop
dir 3
cycle 1 of 30
95.0
0h 5m 0s
C programs
D +
T1 THERMOCYCLER Manual ____________________________________________________ 22
The elapsed time versus programmed hold time is displayed
7.3 View remaining run time
Display during operation:
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
49.9 55.0 60.0
0h 0m23s
104.9°C
B start/stop
dir 3
cycle 1 of 30
50.0 55.0 60.0
0h 5m 0s
C programs
D +
Press [A info] to call up the current remaining time calculation.
Additional information about active pgm
Status:
Step 2
Cycle 3 of 30
Remaining time: 2h 57m
Stop or pause: key B in main menu
A
B
C quit
D
The current remaining run time id displayed in line 4.
7.4 Pause / stop program
Main display during operation:
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
72.0°C
0h 0m18s
104.8°C
B start/stop
dir 3
cycle 8 of 30
72.0
0h 0m30s
C programs
D +
To pause or stop the active program press [B start/stop]
Stop/pause
directory:
program no:
A ?
Select
[B pause]
[D stop]
[C quit ]
3
0 test 1
B pause
C quit
to pause program
to stop program
to return to the program display
When you select [B pause], the following screen is displayed:
D stop
T1 THERMOCYCLER Manual ____________________________________________________ 23
Prog
Step
temp[°C]
time
lid
A info
0 TEST 1
1
72.0°C
104.8°C
B start/stop
dir 3
cycle 8 of 30
72.0
pause
C programs
D +
The program pauses in the current state. The word pause is displayed and alternates with the
elapsed time. You can proceed with the program with [B start/stop].
T1 THERMOCYCLER Manual ____________________________________________________ 24
8
Special functions
Main screen
Prog
step
temp[°C]
time
lid
A ?
25.2°C
25.4°C
B start/stop
C programs
D +
To change special features of the T1 THERMOCYCLER press [D +].
1
2
3
4
5
print
signal
language
standard mode
test mode
A back
B forward
Scroll though the list with the cursor keys
8.1
C quit
D enter
. Enter desired function with [D enter]
Print protocols
1 graphic protocol*
2 graphic protocols*
3 print program
4 print all programs
A
B
C quit
Scroll through the list with the cursor keys
enter].
D enter
. Confirm the highlighted function with [D
8.2 Switch beep on/off
There is an option for an audible signal whenever a program enters a pause and when the
program is finished. If this option is active, the beep can be switched off by pressing any key of
the keyboard.
with signal
A
yes
no
B
C quit
D enter
In this window you can select whether there is a signal or whether there is not. Using the cursor
keys you can toggle between signal yes/no. Confirm your selection with [D enter].
T1 THERMOCYCLER Manual ____________________________________________________ 25
8.3 Select language
The display of the T1 THERMOCYCLER can be switched from English to German. Select language
with cursor keys
. As you toggle between the two settings the language will change.
language
A
German
English
B
To confirm your selection press [D enter]
C quit
D enter
T1 THERMOCYCLER Manual ____________________________________________________ 26
9
Sealing of microplates
Note: For sealing of microplates we recommend 96 well HSQ (high speed quality) plates (050230) and heat sealing film (050-234).
Note: There is no special program required for heat sealing. The hard - and plastic ware is
designed to provide reliable sealing with a lid temperature at 110°C.
9.1
To seal microplates in the cycler
Enter a program and set lid temperature to 110 °C. (we recommend an initial denaturation
step of 4 minutes at 94°C).
Load microplate and place it into the cycler.
Cover plate with heat sealing film.
Important: The dull side of the film must face towards the microplate!
Close the lid and turn down the wheel until the optimum pressure is applied (you will hear a
clicking noise as soon as the clutch mechanism starts)
start cycler program
9.2
Remove heat sealing film
To remove the heat sealing film, heat the top of the plate in your thermocycler. Set heated lid to
110°C and incubate microplate 2-5 minutes.
T1 THERMOCYCLER Manual ____________________________________________________ 27
10 Maintenance
The T1 THERMOCYCLER was built to operate for a long time without the need for periodical
maintenance. The housing may be cleaned from time to time with a smooth cotton cloth. Do not
use strong detergents or organic solvents for cleaning.
11 Exchange of modules
For exchange of modules you need two hexagonal keys (number 3 and number 5). These keys
are included in the shipment of the module.
Remove module
Remove power plug
Place instrument upside down on a soft surface
Remove the 2 screws that close the shutter (hexagonal key number 3)
Remove the 4 screws that hold the block (hexagonal key number 4)
unplug connectors from the block (control and power supply)
remove module
Install module
Follow description in reversed order.
T1 THERMOCYCLER Manual ____________________________________________________ 28
12 Trouble shooting
12.1 Slow heating and cooling
The T1 THERMOCYCLER is equipped with a strong ventilator for the cooling of the heat sink. The
inlet of this fan is located at the bottom side of the instrument. Be sure that the inlet is not
clogged by dust or other material (e.g. a sheet of paper placed under the cycler can be attached to
the inlet as the fan is in operation). Dirt can be removed easily from the inlet with a conventional
vacuum cleaner.
12.2 Adaptation of protocols from other cyclers
Since the T1 THERMOCYCLER is an extremely fast thermocycler it may be necessary to reduce
the heating and cooling ramps to run protocols from other cyclers. For the setting of the heating
and cooling ramps see section 6.3. Alternatively, the time settings may be extended.
Note: Protocolls that have been optimized with a TGradient thermocycler can be run without
changes.
T1 THERMOCYCLER Manual ____________________________________________________ 29
13 Appendix
13.1 Technical specification T1 Thermocycler 96
Thermal range
-3°C – 100°C
Temperature accuracy
+ 0.1°C
Thermal homogeneity
+ 0.3°C within 15 sec.
Maximum heating speed
4°C/sec.
Max. cooling speed
3°C/sec.
Sample capacity
96 x 0.2ml tubes, 96 well microplates, strips
Line Voltage
115 Volt or 230 Volt
Frequency
50-60 Hz
Power consumption
310 watts maximum
Fuses
see label on the back of the instrument
Display
LCD graphical display, back-lit
Ports
serial: RS232 (bi-directional data transfer)
parallel: Centronics (printer)
Port for in tube-sensor
memory
Total capacity: 1500 steps
in 10 directories with maximum 100 programs each
Dimensions (WxDxH)
Weight
Storage conditions
Operating conditions
25 x 34 x 16,5 cm
8,8 kg
5°C – 35°C
10 – 90% humidity
13.2 Ordering information
T1 THERMOCYCLER
T1 THERMOCYCLER 48 for 48 x 0.5ml tubes
T1 THERMOCYCLER 96 for 96 x 0.2ml tubes, strips or 96 well microplates
T1 THERMOCYCLER 384 for 384 well microplates*
T1 THERMOCYCLER in situ for 4 microscopic slides*
Order-No.
050-800
050-801
050-802
050-803
T1 THERMOCYCLER Modules
T1 THERMOCYCLER 48 Module for 48 x 0.5ml tubes
T1 THERMOCYCLER 96 Module for 96 x 0.2ml tubes, strips or 96 well
microplates
T1 THERMOCYCLER 384 Module for 384 well microplates*
T1 THERMOCYCLER in situ Module for 4 microscopic slides*
available 12/99
050-810
050-811
050-812
050-813
For information about tubes, strips, microplates and other consumables please contact
BIOMETRA or your local BIOMETRA distributor.
T1 THERMOCYCLER Manual ____________________________________________________ 30
14 Instructions for return shipment
In case of faults occurring with our T1 THERMOCYCLER please proceed as follows:
• Return only defective devices. For technical problems which are not definitively recognizable
as device faults please contact the Technical Service Department at Biometra ( Tel.:
+49/551/5068640, Fax: ++49/551/5068666, e-mail: [email protected]).
• Important: Cleanse all parts of the instrument of biologically dangerous, chemical or
radioactive contaminants. If a an instrument is contaminated, Biometra will be forced to
refuse to accept the device. The sender of the repair order will be held liable for possible
losses resulting from insufficient decontamination of the device.
• Please prepare written confirmation that the device is free from biologically dangerous and
radioactive contaminants. The declaration of decontamination (see 16) has to be attached to
the outside of the packaging.
• Use the original packing material. If not available, please use a comparable robust box. Be
sure that the instrument fits tightly into the box. When packaging, protect the device in a way
to avoid scratches.
• Label the outside of the box with “CAUTION! SENSITIVE ELECTRONIC
INSTRUMENT!”
• Please enclose a note which contains the following:
a) Sender’s name and address,
b) Name of a contact person for further inquiries with telephone number,
c) Description of the fault, which also reveals during which procedures the fault occurred, if
possible
T1 THERMOCYCLER Manual ____________________________________________________ 31
15 Warranty
This Biometra instrument has been carefully built, inspected and quality controlled before
dispatch. Hereby Biometra warrants that this instrument conforms to the specifications given in
this manual. This warranty covers defects in materials or workmanship for 12 month as
described under the following conditions:
This warranty is valid for 12 month from date of shipment to the customer from Biometra or an
authorized distributor. This warranty will not be extended to a third party without a written
agreement of Biometra.
This warranty covers only the instrument and all original accessories delivered with the
instrument. This warranty is valid only if the instrument is operated as described in the manual.
Biometra will repair or replace each part which is returned and found to be defective. This
warranty does not apply to wear from normal use, failure to follow operating instructions,
negligence or to parts altered or abused.
T1 THERMOCYCLER Manual ____________________________________________________ 32
16 Equipment Decontamination Certificate
To enable us to comply with German law (i.e. §28 StrlSchV, §17 GefStoffV and §19 ChemG) and to avoid
exposure to hazardous materials during handling or repair, will you please complete this form, prior to the
equipment leaving your laboratory
COMPANY / INSTITUTE ____________________________________________________________
ADDRESS ____________________________________________________________
TEL NO _____________________ FAX NO _____________________ E-MAIL _____________________
______________________________
EQUIPMENT
If on loan / evaluation
Model
Serial No
__________
__________
__________
__________
__________
__________
__________
__________
Start Date: ________ Finish Date ________
Hazardous materials used with this equipment
_________________________________________________________________________________
_________________________________________________________________________________
_________________________________________________________________________________
Has the equipment been cleaned and decontaminated? YES / NO (delete)
Method of cleaning / Decontamination
_________________________________________________________________________________
_________________________________________________________________________________
_________________________________________________________________________________
NAME ________________________________ POSITION __________________________
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
SIGNED ______________________________ DATE ______________________________
PLEASE RETURN THIS FORM TO BIOMETRA GmbH OR YOUR LOCAL BIOMETRA DISTRIBUTOR
TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS WITHOUT THIS
CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
T1 THERMOCYCLER Manual ____________________________________________________ 33
17 Year 2000 conformity declaration
YEAR 2000
Conformity Declaration
We confirm that the
T1 THERMOCYCLER
is YEAR 2000 compatible
In connection with the YEAR 2000 we guarantee that the above
mentioned instruments are produced in such a way that they can be
used before, during and after calendar YEAR 2000 and that they work
properly without failures concerning date fields or functions referring
to date fields.
In particular we guarantee that
♦ the instruments perform all functions without system crashes and abnormal behavior
despite the YEAR 2000 problem,
♦ software integrated into the instruments was developed in a way that YEAR 2000
compatibility is warranted,
♦ integrated software interprets rules concerning centuries and multiple centuries (like
leap years) properly and does not cause system crashes and failure functions,
♦ integrated computer chips are YEAR 2000 compatible,
♦ the date 9. 9. 1999 does not cause system crashes and failure functions.
Dr. Jürgen Otte
Quality Manager
Dr. Lutz Heine
Quality Manager Assistant
T1 THERMOCYCLER Manual ____________________________________________________ 34
18 EU - Declaration of Conformity
EU - Konformitätserklärung
Göttingen, den 09.03.1999
im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung innerhalb
bestimmter Spannungsgrenzen 73/23/EWG, Anhang III
following the EC directive about electrical equipment for use within certain limits of voltage
73/23/EWG, appendix 3
und / and
im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit 89/336/EWG, Anhang I.
following the EC directive about theelectromagnetic compability 89/336/EWG, appendix 1.
Hiermit erklären wir, daß folgende Thermocycler und Thermocycler Module:
Herewith we declare that the following thermocyclers and thermocycler moduls:
Typen:
types:
T1 Thermocycler 48, T1 Thermocycler 96,
T1 Thermocycler 384, T1 Thermocycler Modul 48,
T1 Thermocycler Modul 96, T1 Thermocycler Modul 384
T1 Thermocycler 48, T1 Thermocycler 96,
T1 Thermocycler 384, T1 Thermocycler Modul 48,
T1 Thermocycler Modul 96, T1 Thermocycler Modul 384
Best.-Nr. / Order No.: 050-900, 050-901, 050-902, 050-903, 050-910, 050-911, 050-912, 050-913
den grundlegenden Anforderungen der
corresponds to the basic requirements of
EG-Niederspannungsrichtlinie 73/23 EWG i.d.F. 93/68 EWG und der
EC low voltage directive 73/23 EWG in version 93/68 EWG and the
EG-Richtlinie über die elektromagnetische Verträglichkeit 89/336 EWG i.d.F. 93/68 EWG
entsprechen.
EC directive about the electromagnetic compatibility 89/336/EWG in version 93/68 EWG.
Folgende harmonisierte Normen wurden angewandt:
The following harmonized standards have been used:
EN 50081-1
EN 60555-2
EN 61010-1
EN 50082-1
EN 60555-3
EN 61010-2
....................................................
Dr. Jürgen Otte, Quality Manager
T1 THERMOCYCLER Manual ______________________________________________________I
1
INTRODUCTION ............................................................................................................................................. 2
2
BEFORE YOU START .................................................................................................................................... 3
2.1
2.2
3
FIRST STEPS WITH THE T1 THERMOCYCLER ..................................................................................... 4
3.1
3.2
3.3
3.4
3.5
3.6
4
SELECT AND START PROGRAM ....................................................................................................................... 20
DISPLAY DURING OPERATION ........................................................................................................................ 21
VIEW REMAINING RUN TIME .......................................................................................................................... 22
PAUSE / STOP PROGRAM ................................................................................................................................ 22
SPECIAL FUNCTIONS ................................................................................................................................. 24
8.1
8.2
8.3
9
PROGRAM TIME INCREMENTS ........................................................................................................................ 18
PROGRAM TOUCH DOWN................................................................................................................................ 19
ADJUST HEATING AND COOLING RAMPS ........................................................................................................ 19
RUN PROGRAM ............................................................................................................................................ 20
7.1
7.2
7.3
7.4
8
DELETE / INSERT PROGRAM STEPS ................................................................................................................. 14
COPY PROGRAM............................................................................................................................................. 15
DELETE PROGRAM ......................................................................................................................................... 16
FURTHER PROGRAMMING OPTIONS ................................................................................................... 18
6.1
6.2
6.3
7
SELECT A DIRECTORY ...................................................................................................................................... 8
SELECT A PROGRAM STORE.............................................................................................................................. 8
ENTER NAME FOR SUBDIRECTORY ................................................................................................................... 9
ENTER A PROGRAM NAME ................................................................................................................................ 9
ENTER THE LID TEMPERATURE ...................................................................................................................... 10
SELECT / DESELECT PRE-HEATING OF THE LID ............................................................................................... 10
ENTER TEMPERATURE AND TIME SETTINGS ................................................................................................... 11
SET CYCLE NUMBER ...................................................................................................................................... 12
COOL BELOW AMBIENT TEMPERATURE .......................................................................................................... 12
SAVE PROGRAM ........................................................................................................................................ 13
VIEW/EDIT PROGRAM DATA................................................................................................................... 14
5.1
5.2
5.3
6
THE T1 THERMOCYCLER FRONT VIEW ............................................................................................................ 4
THE T1 THERMOCYCLER REAR VIEW .............................................................................................................. 5
THE T1 THERMOCYCLER CONTROL PANEL ...................................................................................................... 6
INITIAL SELF TEST............................................................................................................................................ 7
THE T1 THERMOCYCLER DISPLAY ................................................................................................................... 7
HANDLING OF THE ADJUSTABLE HEATED LID .................................................................................................. 7
CREATING A PROGRAM.............................................................................................................................. 8
4.1
4.2
4.3
4.4
4.5
4.6
4.7
4.8
4.9
4.10
5
SAFETY WARNINGS .......................................................................................................................................... 3
WARNING SYMBOLS ........................................................................................................................................ 3
PRINT PROTOCOLS ......................................................................................................................................... 24
SWITCH BEEP ON/OFF ..................................................................................................................................... 24
SELECT LANGUAGE........................................................................................................................................ 25
SEALING OF MICROPLATES .................................................................................................................... 26
9.1
9.2
TO SEAL MICROPLATES IN THE CYCLER ......................................................................................................... 26
REMOVE HEAT SEALING FILM ........................................................................................................................ 26
10
MAINTENANCE ............................................................................................................................................ 27
11
EXCHANGE OF MODULES ........................................................................................................................ 27
12
TROUBLE SHOOTING................................................................................................................................. 28
12.1
12.2
SLOW HEATING AND COOLING .................................................................................................................. 28
ADAPTATION OF PROTOCOLS FROM OTHER CYCLERS ................................................................................ 28
T1 THERMOCYCLER Manual _____________________________________________________ II
13
APPENDIX ...................................................................................................................................................... 29
13.1
13.2
TECHNICAL SPECIFICATION T1 THERMOCYCLER 96 ................................................................................. 29
ORDERING INFORMATION ......................................................................................................................... 29
14
INSTRUCTIONS FOR RETURN SHIPMENT ........................................................................................... 30
15
WARRANTY ................................................................................................................................................... 31
16
EQUIPMENT DECONTAMINATION CERTIFICATE............................................................................ 32
17
YEAR 2000 CONFORMITY DECLARATION ........................................................................................... 33
18
EU - KONFORMITÄTSERKLÄRUNG ....................................................................................................... 34
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