2015 Product Catalogue

2015 Product Catalogue
EXPERTS in
Electrophoresis
PRODUCT
Catalogue
2015
www.cleaverscientific.com
Contents
Horizontal Gel Systems
Welcome to Cleaver Scientific
2014 is our 10th Anniversary and I am delighted to introduce to you the latest product catalogue
from Cleaver Scientific Ltd for 2015. This is the fifth edition since our company was incorporated, just
10 years ago. Now even larger, it offers a range of instruments, chemicals and consumables for both
electrophoresis and indeed the life science laboratory.
Much of the catalogue growth has come from creative and innovative product additions, including our
patented COMPAC-50™, which we designed and manufactured in conjunction with the University
of Leicester. We are proud that this edition contains significantly more product and technical content
than before. We believe that this will be invaluable to both researchers and dealers for product
selection as well as everyday reference.
For those of you who know us and have used our products previously, you will be pleased to see that
the latest product additions uphold the same high standards for innovation and quality that we are
known for. All of our products, including our flagship gel electrophoresis systems, are supplied directly
from our manufacturing facility in Rugby, based within the heart of the United Kingdom and central to
all major international road, rail and air transport networks. Each product represents the culmination of
the combined creativity, technical and engineering expertise acquired over many years by our in-house
manufacturing and scientific product development team.
Vertical Gel Systems
46-71
Introduction46-47
Selection Guide
48-49
omniPAGE Mini
50-51
octi-PAGE52-53
omniPAGE Mini wide
54-55
VS20WAVE maxi
56-57
Complete Modular Systems
58
VS20WAVE complete electrophoresis system
59
omniPAGE 2D systems
60
VS20WAVE 2D Tube Gel System
61
Isoelectric Focusing
62-63
Second Stage 2D
64
Gradient Mixers
65
Vertical Electrophoresis packages
66-67
Large Format Vertical
68-69
Denaturing Gradient Gel Electrophoresis
70-71
In all of these products, our philosophy remains that we are committed to offering the highest quality
products with the latest technical innovations to make the researcher’s life that little bit easier, but also
more affordable, particularly in these price-sensitive times.
Quality may be a much misused word, but at Cleaver Scientific it defines what we do, by the timely
manufacture and supply of products to our customers that not only fulfil their purpose, but will remain
durable and free of imperfections for many years to come. Our accredited ISO9001/2008 quality
management system and adherence to this ensures that these principles are met.
Please read on to see the exciting products we have to offer.
Adie Cleaver
Managing Director
Cleaver Scientific Ltd.
Blotting Systems
Introduction and Selection Guide
omniPAGE Mini Complete Electroblotting System
omniPAGE Mini Wide Complete Electroblotting System
VS20WAVE Electroblotting System
Tank Sub Electroblotters
omniPAGE Sub Blot System
Sub Blot Mini and WAVE High Intensity Transfer Systems
Semi Dry and Dot and Slot Blotters
Power Supplies
Cleaver Headquarters UK
4-45
Introduction4-5
Selection Guide
6-7
multiSUB™ Mini
8-9
multiSUB™ Midi
10-11
multiSUB™ Choice
12-13
multiSUB™ Maxi
14-15
multiSUB™ Screen
16-17
miniRapide18
Battery Gel
19
multiSUB™ Midi 96
20-21
multiSUB™-422
Casting Systems
23
ClearSight Electrophoresis
24
Accessories25
Package Deals
26-27
Safe Series
28-29
runDOC30
runSAFE31
runVIEW™ MIDI & MINI Systems
33
Teaching Gel Tanks
34-35
Comet Assay Tanks
37
COMPAC-50™ High Throughput Comet
Electrophoresis System
38-39
Cellas Electrophoresis System
40-45
72-81
72-73
74
75
76
77
78
79
80-81
82-89
Introduction82-83
Selection Guide
84-85
nanoPAC-300 & 500 Powers Supplies
86
omniPAC Power Supplies
87-88
Consort Power Supplies
89
Some of the Cleaver Team
Certificate No.17533
Gel Documentation
90-109
Introduction90-91
Selection Guide
92-93
microDOC™95
omniDOC and omniDOC-i
96-99
Uvitec Gel Documentation Sysyems
100
UV Crosslinker
101
UV Transilluminators
102
safeVIEW103
duoVIEW104
microSCAN 2-D and 1-D A4 Gel Scanner
105
Gel Analysis Software
106-109
General Lab Equipment
110-133
Introduction110-111
Pipettes112-113
Quickspin and CSL Multifuge
114-115
RMC24 Refrigerated Micro Centrifuge
116-117
Vortex Mixer
118
Blot Boxes
119
MiniMix 3D Shaker
119
microBLOCK and Cube Dry Baths
120-121
GTC96S Thermal Cycler
122-123
Heaters and Stirrers, Stirring Water Baths and
Peristaltic Pumps
124-125
hybridPRO Hybridisation Shaking Incubators
126-127
UV Sterilisation Cabinets
128
Consort pH Meters, Coolcube and Ice Buckets
129
CSL Chiller
130
Gel Dryers and Vacuum Pumps
131
Rockers and Shakers
132-133
Reagents and Chemicals
134-145
Introduction134-135
Horizontal Nucleic Acid Electrophoresis
136
CleverGEL Instant Agarose Tablets
137
Powdered and Liquid Buffers
138
Ready to Use DNA Markers
139
DNA and Orange G Loading Dye
140
RNase-Free Water and BP Grade Ultra Pure Water
140
GelX Disposable Gel Excision Tips
141
PINK Plus and BLUE Wide Protein Markers
142
Page Buffers
143
Membranes and Stains
144
‘Just Add DNA’ PCR® MaterMiX
145
Complimentary Products
146-153
Introduction146-147
Radiation Safety
148-151
Gloveboxes152
Adam Equipment Balances
153
Terms and Conditions
155
The multiSUB™ Range – Key Features
HORIZONTAL
GEL
SYSTEMS
Cleaver Scientific’s multiSUB™ horizontal gel electrophoresis units have been designed by scientists with the laboratory
scientist in mind. Through its advanced features each unit provides an easy to use, versatile and flexible system to evolve
and adapt with the changing needs of today’s laboratory researcher.
Tank and Lid Design
High quality injection moulded
construction and durable leakproof design for complete safety
and longevity
Cassette-style electrodes – difficult
to break, but inexpensive and easy
to change – composed of 99.99%
corrosion-resistant, pure platinum
Although a long-established technique, horizontal gel
electrophoresis offers many advantages for nucleic acid
separation and remains today one of the mainstays of
molecular biology. Cleaver Scientific’s multiSUB™ range
offers the most comprehensive and versatile line of horizontal
gel electrophoresis units currently available for low – and
high-throughput DNA and RNA applications.
Electrical safety – lid removal immediately disconnects power to the lower buffer chamber to allow entirely
safe access to the gel
Easy-click lid removal – asymmetric lid design and thumb locators on colour-coded cassette-style electrodes
ensure that electrophoresis is always performed in the correct polar direction – i.e. negative to positive
Combs
The widest range of combs available of any gel tank manufacturer - fit virtually every application from
preparatory electrophoresis to high-throughput screening
Available in four thicknesses and colour-coded. Range from:
• White – 1mm supplied as standard
• Black – 0.75mm for tightly resolved bands
• Red – 1.5mm to maximise sample volume
• Blue – 2mm to maximise sample volume
Selection Guide
6-7
multiSUB™ Horizontal Gel Systems
Black and white combs recommended for high resolution gels and publication quality data; red and blue to
scale-up nucleic acid volumes for preparatory techniques
8-25
Package Deals Horizontals
26-27
SAFE Series
28-33
Teaching Gel Tanks
34-35
Multiple gel tray options – eliminate the need for additional gel tanks and allow gels to be cast externally,
keeping the tank permanently in use for electrophoresis if required
Comet Assay
36-37
UV and blue light transparent
New COMPAC-50™ High Throughput Comet
Electrophoresis Systems
38-39
Height-adjustable, without any requirement for specialist tools or comb holders, to give user full control
over well depth and sample-loading volume; rigid comb back prevents heat-induced warping
Reversible loading guides sit directly above each well to provide a convenient loading template for singleand multi-channel pipettes
Trays
Casting
‘Plug-and-Go’ casting – moulded casting dams clip easily onto the ends of the gel tray for rapid external
casting, allowing the multiSUB™ unit to remain in use for gel running.
Clinical Electrophoresis
Casting is as simple as 1, 2, 3...Simply place one dam onto the lab bench facing upwards and insert the tray
into the groove in the dam(1) and repeat with the second dam at the other end(2). The tray is now sealed and
may be placed on flat bench space or gel levelling table in readiness for leak proof gel-casting (3).
40-45
Other casting options include flexicaster and plastic casting gates - see multiSUB™ Selection Guide
RELATED PRODUCTS
Accessories
Red loading guides – aid well and sample visualisation during loading (1).
White gel platform – provides a contrasting background to view bromophenol blue migration fronts and
determine electrophoresis progress during every run (2).
Gel levelling table – recommended especially for MSMAXI or MSSCREEN gel trays. Adjustable levelling feet
used in conjunction with a levelling bubble provide an even surface upon which to pour wide- and largeformat gels, to ensure consistent and uniform migration
Power Supplies
PAGES 82-89
reagents and
chemicals PAGEs 134-145
ready to uSe dna
marker PAGE 139
runFAST cool pack and platform – sit directly above the gel in the buffer to provide enhanced resolution
and faster run times; especially suited to larger format horizontals. To use: fill the tank with buffer and load
samples (1.); insert platform above the gel (2.); place pre-frozen cool pack onto platform (3.); connect to
power supply and run samples at higher voltage (4.).
Power cables – with 4mm connectors compatible with most modern low-to-medium voltage power
supplies; CE compliant (1). Adaptors available for complete power supply compatibility.
Buffer Saver Blocks – conserve buffer for added economy – especially beneficial in larger format MSMAXI
and MSSCREEN units (2).
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6
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 7
Selection Guide
MSMINI
MSMIDI
MSCHOICE
MSCHOICEST
MSMAXI
MSSCREEN
FMMS10
MSBGEL
MSMIDI96
MULTISUB-4
The preferred option for
quick sample-checks,
particularly following restriction digestion during
cloning. Its slim tray format makes the MSMINI
a very economical choice
for separation of up to 64
samples.
These units offer the
same trays lengths, but
in a wider format, to run
more samples just as
economically as in the
MSMINI under similar
running conditions.
Wide-format system with
2-3 times the sample
capacity as the MSMIDI
and MSMINI units. A
comprehensive selection
of combs provides full
flexibility for preparatory
and screening techniques.
Suitable for cloning and
mini-prep analysis.
A ‘stretched’ version of
the MSCHOICE, this
system is perfect for
extended high-resolution
separations of samples
from 96-well microplates
and blocks, using its
15x20cm and 15x25cm
gel trays and four
28-sample multichannel
compatible combs. May
also be used for routine
preparatory techniques.
Large-format system
versatile enough to
be used for routine
preparatory and
high-throughput
techniques. Combs with
spacing compatible
with fixed multichannel pipettes simplify
loading from 96-well
microplates and thermal
cycler blocks. Suitable
for RFLP analysis,
Southern and northern
blotting applications,
and DNA library and
PCR fragment analyses.
Supplied with six
28-sample multichannel compatible combs,
with well-spacing that
favours direct loading.
The content of at least
one 96-well microplate or
thermal cycler block may
be screened in a single
gel. Standard combs
may be exchanged for
56-sample versions to
double the sample capacity. A dedicated system
for all high-throughput
screening applications.
An ultra-compact
self-contained system for
routine molecular biology
procedures and quick
checks of samples. Buffer
and gel volumes kept to
a minimum to maximise
current and separation
speed.
Designed specifically
with safety and cost
constraints in mind,
this system is ideal for
education as no power
supply is required – only
batteries. Educational
kits are available which
include: protocols,
non-toxic electrophoretic dyes, agarose and
buffers.
Separates up to 1200
samples in as little as 15
minutes, over a short
1cm separation distance,
in 4 stacked horizontal
gel trays. A relatively
small footprint area,
plus the capacity to run
4 gels in 1 tank, saves
on valuable laboratory
bench space as well as
resources.
12.5 x 22 x 9cm
17.5 x 26.5 x 9cm
17.5 x 41 x 9cm
23 x 39.5 x 9cm
96-well comb block
and specialised tray
replicate the format
and well-spacing of
microplates and thermal
cycler blocks. Offset
well configuration
and short separation
distance (1.8cm)
allow samples to be run
within 15-30 minutes.
Ideal system for rapid
screening of up to 96
PCR-fragment length
polymorphisms. Stretch
versions available with
extended 3.6cm separation distance to double
capacity.
10x7cm / MS10-UV7
10x10cm / MS10-UV10
15x7cm / MS15-UV7
15x10cm / MS15-UV10
15x15cm / MS15-UV20
15x20cm / MS15UVST20
15x25cm / MS15UVST25
20x10cm / MS20-UV10
20x15cm / MS20-UV15
20x20cm / MS20-UV20
20x25cm / MS20-UV25
Unit Dimensions
28 x 50 x 9cm
(w x l x h)
15 x 15 x 4cm
17 x 11 x 3.5cm
12.5 x 22 x 9cm
(MIDI96)
12.5 x 46.5 x 8cm
(MIDI96ST)
11 x 35 x 16cm
1-32 (7x7cm)a
Sample Capacity
1-64 (7x10cm)b
1-50 (10x7cm)a
1-100 (10x10cm)b
1-70 (15x7cm)a
1-140 (15x10cm)b
1-210 (15x15cm)c
1-280 (15x20cm)†
1-350 (15x25cm)††
1-200 (20x10cm)b
1-350 (20x15cm)c
1-450 (20x20cm)d
1-550 (20x25cm)e
Active Gel
Size (w x l) /
Corresponding
Gel Tray
10x8cm / in-built tray
6x7.5cm / in-built tray
10x12cm / MS10-UV96
10x24cm / MS10UV96ST
8x6cm / MSUB4UV6
8x12cm / MSUB4UV12
8x18cm / MSUB4UV18
Tank Buffer
Volume
300ml
500ml
1000ml
1500ml
96 samples plus
12 (1 lane) or 24 (2
lanes) marker wells
8-126 (8x6cm)g
8-198 (8x12cm)e
8-306 (8x24cm)h
200ml: 1 tray
400ml: 2 trays
600ml: 3 trays
800ml: 4 trays
No
Unit Dimensions
9 x 21 x 9cm
(w x l x h)
Active Gel
Size (w x l) /
Corresponding
Gel Tray
Buffer
Recirculation
7x7cm / MS7-UV7
7x10cm / MS7-UV10
225ml
No
No
Plug-and-Go
Casting Dams
Supplied
Yes,
1 pair
Yes,
1 pair
Flexicaster
Options &
Tray Capacity
MS7/10-FC: 1 tray
MS15/20-FC: 2 trays*
MS26-FC: 3 trays*
MS7/10-FC: 1 tray
MS15/20-FC: 1 tray
MS26-FC: 3 trays*
Typical Running
80V, 45-60 minutes
Conditions
Bromophenol
~4-5cm/h at 80V
Blue Migration
Recommended for high voltage applications or extended runs. Requires
modified lid with 2 buffer recirculation ports. Available on request as per part
numbers below: MS15LID-BP
MS15STLID-BP
MS20LID-BP
Yes,
1 pair
Yes,
1 pair
Yes,
1 pair
MS15/20-FC: 1 tray
MS26-FC: 1 tray
90V, 45-60 minutes
90-150V, 60-90 minutes
100-150V, 60-90
minutes
100-150V,60-90 minutes
~4-5cm/h at 90V
~4-7cm/h at 90-150V
~4-6cm/h at 100-150V
~4-6.5cm/h at 100-150V
26x16cm / MS26-UV16
26x24cm / MS26-UV24
26x32cm / MS26-UV32
28-336 (26x16cm)c
28-504 (26x24cm)e
Sample Capacity 28-672 (26x32cm)f
1-40 (10x8cm)a
4-32 (6x7.5cm)a
Tank Buffer
Volume
2000ml
50ml
50ml
300ml (MSMIDI96)
700ml (MIDI96ST)
Buffer
Recirculation
Yes – buffer recirculation ports included as
standard
No
No
No
Plug-and-Go
Casting Dams
Supplied
No – supplied with
dedicated MSSCREEN
Yes,
No – plastic casting gates No – plastic casting gates
flexicaster; See MS26-FC,
1 pair
pg 23
No
Flexicaster
Options &
Tray Capacity
MS26-FC: 1 tray
No
No
No
MSUB4EXCASTER: 4
trays
Typical Running 100-150V,
Conditions
90-120 minutes
50V,
30-60 minutes
12V,
1-3 hours
90V,
15-30 minutes
100-150V,
15-30 minutes
* Assumes multiSUB™ trays are of the same length (e.g two MS7-UV7) and arranged side-by-side.
Bromophenol
Blue Migration
~4cm/hr at 50V
~1cm/hr at 12V
~4-5cm/h at 90V
~4-6cm/hr at 100-150V
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a Assumes 1-2 combs per gel; b Assumes 1-4 combs per gel; c Assumes 1-6 combs per gel; d Assumes 1-9 combs per gel; e Assumes 1-11 combs per gel; f Assumes 1-12 combs per gel
† Assumes 1-8 combs per gel; †† Assumes 1-10 combs per gel
~4-6cm/hr at 100-150V
8
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 9
MultiSUB™ MINI COMBS
ORDERING INFORMATION
Sample
volume
for a 5mm
thick gel
MSMINI7
multiSUB Mini, 7 x 7cm UV Tray, 2 x 8 sample
combs, loading guides and dams
Code
Description
MSMINI10
multiSUB Mini, 7 x 10cm UV Tray, 2 x 8 sample
combs, loading guides and dams
MS7-1-0.75
Comb Prep 1, Marker 1, 0.75mm
thick
152µl
MSMINIDUO
multiSUB Mini, 7 x 7cm & 7 x 10cm UV Tray,
2 x 8 sample combs, loading guides and dams
MS7-2-0.75
Comb Prep 2, Marker 2, 0.75mm
thick
68µl
MS7-UV7
7 x 7cm UV Tray
MS7-4-0.75
Comb Prep 4, Marker 2, 0.75mm
thick
36µl
MS7-UV10
7 x 10cm UV Tray
MS7-8MC-0.75
Comb 8 sample MC, 0.75mm thick
8µl
MS7-PE
Positive Electrode
MS7-8-0.75
Comb 8 sample, 0.75mm thick
19µl
MS7-NE
Negative Electrode
MS7-10-0.75
Comb 10 sample, 0.75mm thick
14µl
MS7-UVDAM
Casting Dams
MS7-12MC-0.75
Comb 12 sample MC, 0.75mm thick
10µl
MS7/10-FC
multiSUB Mini/Midi Flexi caster
MS7-16-0.75
Comb 16 sample, 0.75mm thick
7µl
MS7-LG
Adhesive Loading Guides
MS7-WP
Viewing Platform
MS7-1-1
Comb Prep 1, Marker 1, 1mm
thick
203µl
MSMINICP
Cool-pack and Platform
MS7-2-1
90µl
MSMINIBSB
Buffer Saver Blocks, pk/2 saves 100ml of buffer
Comb Prep 2, Marker 2, 1mm
thick
MS7-UVS
7cm UV Gel Scoop
MS7-4-1
Comb Prep 4, Marker 2, 1mm
thick
48µl
CSL-CAB
Electrophoresis cable (Black & Red)
MS7-8MC-1
Comb 8 sample MC, 1mm thick
11µl
MS7-8-1
Comb 8 sample, 1mm thick
25µl
MS7-10-1
Comb 10 sample, 1mm thick
18µl
MS7-12MC-1
Comb 12 sample MC, 1mm thick
14µl
MS7-16-1
Comb 16 sample, 1mm thick
10µl
MS7-1-1.5
Comb Prep 1, Marker 1, 1.5mm thick
304µl
MS7-2-1.5
Comb Prep 2, Marker 2, 1.5mm thick
135µl
MS7-4-1.5
Comb Prep 4, Marker 2, 1.5mm thick
72µl
MS7-8MC-1.5
Comb 8 sample MC, 1.5mm
thick
17µl
MS7-8-1.5
Comb 8 sample, 1.5mm thick
37µl
MS7-10-1.5
Comb 10 sample, 1.5mm thick
27µl
MS7-12MC-1.5
Comb 12 sample MC, 1.5mm
thick
20µl
MS7-16-1.5
Comb 16 sample, 1.5mm thick
15µl
MS7-1-2
Comb Prep 1, Marker 1, 2mm thick
405µl
MS7-2-2
Comb Prep 2, Marker 2, 2mm thick
180µl
MS7-4-2
Comb Prep 4, Marker 2, 2mm thick
96µl
MS7-8MC-2
Comb 8 sample MC, 2mm thick
23µl
MS7-8-2
Comb 8 sample, 2mm thick
50µl
MS7-10-2
Comb 10 sample, 2mm thick
36µl
MS7-12MC-2
Comb 12 sample MC, 2mm thick
27µl
MS7-16-2
Comb 16 sample, 2mm thick
20µl
MSMiniduo complete system
TECHNICAL SPECIFICATIONS
Gel dimensions
(w x l)
7 x 7cm
7 x 10cm
Unit dimensions
(w x l x h)
9 x 21 x 9cm
Max Sample
Capacity
7 x 7cm tray - 32 samples
7 x 10cm tray - 64 samples
Buffer volume
225ml
Combs available:
No. of samples
Thicknesses
1, 2, 4, 8MC, 8, 10, 12MC, 16
0.75, 1, 1.5, 2mm
features:
• Available with
7 x 7cm, 7 x 10cm
or with both
gel trays
• Economic low gel
and buffer volumes
• Small lab bench
footprint
multiSUB™ Mini
The multiSUB™ Mini is the smallest
unit in the range, designed for low
to medium numbers of samples. The
small gel size maximises run economy
but does not compromise versatility
as two tray options are available – 7
x 7cm and 7 x 10cm – and combs
ranging from preparative up to 16
samples. Simply by altering the gel tray
or comb, this compact unit is capable
of resolving up to 64 different samples,
prepping 1ml of sample or separating
sample bands over a distance of 9cm.
Buffer saver blocks physically reduce
the volume of a gel chamber and so
reduce buffer requirements, saving
cost.
Condensation free lids available in all sizes
MultiSUB Mini/Midi Flexi-caster
Typical Applications
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Designed for quick checks of low to
medium numbers of samples
10
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 11
MultiSUB™ MIDI COMBS
ORDERING INFORMATION
Sample
volume
for a 5mm
thick gel
MSMIDI7
multiSUB Midi, 10 x 7cm UV Tray, 2 x 16 sample
combs, loading guides and dams
Code
Description
MSMIDI10
multiSUB Midi, 10 x 10cm UV Tray, 2 x 16
sample combs, loading guides and dams
MS10-1-0.75
Comb Prep 1, Marker 1, 0.75mm
thick
270µl
MSMIDIDUO
multiSUB Midi, 10 x 7cm & 10 x 10cm UV Tray,
2 x 16 sample combs, loading guides and dams
MS10-2-0.75
Comb Prep 2, Marker 2, 0.75mm
thick
118µl
MS10-UV7
10 x 7cm UV Tray
MS10-4-0.75
Comb Prep 4, Marker 2, 0.75mm
thick
57µl
MS10-UV10
10 x 10cm UV Tray
MS10-8-0.75
Comb 8 sample, 0.75mm thick
30µl
MS10-PE
Positive Electrode
MS10-10MC-0.75
Comb 10 sample MC, 0.75mm thick
20µl
MS10-NE
Negative Electrode
MS10-12-0.75
Comb 12 sample, 0.75mm thick
17µl
MS10-UVDAM
Casting Dams
MS10-16-0.75
Comb 16 sample, 0.75mm thick
12µl
MS7/10-FC
multiSUB Mini/Midi Flexi caster
MS10-20MC-0.75
Comb 20 sample MC, 0.75mm thick
10µl
MS10-LG
Adhesive Loading Guides
MS10-25-0.75
Comb 25 sample, 0.75mm thick
7µl
MS10-WP
Viewing Platform
MS10-1-1
Comb Prep 1, Marker 1, 1mm thick
360µl
MSMIDICP
Cool-pack and Platform
MS10-2-1
Comb Prep 2, Marker 2, 1mm thick
158µl
MSMIDIBSB
Buffer Saver Blocks, pk/2 saves 100ml of buffer
MS10-4-1
Comb Prep 4, Marker 2, 1mm thick
77µl
MS10-UVS
10cm UV Gel Scoop
MS10-8-1
Comb 8 sample, 1mm thick
41µl
CSL-CAB
Electrophoresis cable (Black & Red)
MS10-10MC-1
Comb 10 sample MC, 1mm thick
27µl
MS10-12-1
Comb 12 sample, 1mm thick
23µl
MS10-16-1
Comb 16 sample, 1mm thick
16µl
MS10-20MC-1
Comb 20 sample MC, 1mm thick
14µl
MS10-25-1
Comb 25 sample, 1mm thick
10µl
MS10-1-1.5
Comb Prep 1, Marker 1, 1.5mm
thick
540µl
MS10-2-1.5
Comb Prep 2, Marker 2, 1.5mm thick
236µl
MS10-4-1.5
Comb Prep 4, Marker 2, 1.5mm
thick
115µl
MS10-8-1.5
Comb 8 sample, 1.5mm thick
61µl
MS10-10MC-1.5
Comb 10 sample MC, 1.5mm thick
41µl
MS10-12-1.5
Comb 12 sample, 1.5mm thick
34µl
MS10-16-1.5
Comb 16 sample, 1.5mm thick
24µl
MS10-20MC-1.5
Comb 20 sample MC, 1.5mm thick
20µl
MS10-25-1.5
Comb 25 sample, 1.5mm thick
15µl
MS10-1-2
Comb Prep 1, Marker 1, 2mm thick
720µl
MS10-2-2
Comb Prep 2, Marker 2, 2mm thick
315µl
• Run up to 100
samples
MS10-4-2
Comb Prep 4, Marker 2, 2mm thick
153µl
• Low buffer volumes
MS10-8-2
Comb 8 sample, 2mm thick
81µl
MS10-10MC-2
Comb 10 sample MC, 2mm thick
54µl
MS10-12-2
Comb 12 sample, 2mm thick
45µl
• Ideal for rapid
electrophoresis
MS10-6-2
Comb 16 sample, 2mm thick
32µl
MS10-20MC-2
Comb 20 sample MC, 2mm thick
27µl
MS10-25-2
Comb 25 sample, 2mm thick
20µl
MSMidi complete system
TECHNICAL SPECIFICATIONS
Gel dimensions
(w x l)
10 x 7cm
10 x 10cm
Unit dimensions
(w x l x h)
12.5 x 22 x 9cm
Max Sample
Capacity
10 x 7cm tray - 50 samples
10 x 10cm tray - 100 samples
Buffer volume
300ml
Combs available:
No. of samples
Thicknesses
1, 2, 4, 8, 10MC, 12,16, 20MC, 25
0.75, 1, 1.5, 2mm
features:
• Available with
10 x 7cm, 10 x
10cm or with both
gel trays
multiSUB™ Midi
With gel tray options of 10 x 7cm and
10 x 10cm, the multiSUB™ Midi has
been designed for routine horizontal
gel electrophoresis. Extending only the
width of this unit allows more
samples to be resolved per gel than the
multiSUB™ Mini without a significant
increase in buffer
or gel volumes.
A maximum of 100 samples per gel
can be resolved making this unit ideal
for those routinely checking medium
numbers of samples over short to
medium gel run lengths. Loading
guides allow easy well identification
and sample loading. Scoops available
as an option allow safe transfer of
gels. For Power Supplies and Product
Selector see page 84.
Typical Applications
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visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Ideal for quick checks of samples from
PCR and cloning
Condensation free lids available
12
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 13
MultiSUB™ Choice COMBS
ORDERING INFORMATION
MSCHOICE7
multiSUB™ Choice
15 x 7cm UV Tray, 2 x 20 sample combs,
loading guides and dams
MSCHOICE10
multiSUB™ Choice
15 x 10cm UV Tray, 2 x 20 sample combs,
loading guides and dams
MSCHOICE15
multiSUB™ Choice
15 x 15cm UV Tray, 2 x 20 sample combs,
loading guides and dams
MSCHOICETRIO
multiSUB™ Choice
Trio
15 x 7,10 & 15cm UV Tray, 2 x 20 sample
combs, loading guides and dams
MSCHOICETRIO15
3 x 15cm UV tray, 2x 20 sample combs,
multiSUB™ Choice
loading guides and dams
Trio 15
MSCHOICEST20
multiSUB™ Choice
Stretch 20
MSCHOICEST25
multiSUB™ Choice
Stretch 25
15 x 20cm UV tray, 4 x 28 sample combs,
loading guides and dams
15 x 25cm UV tray, 4 x 28 sample combs,
loading guides and dams
TECHNICAL SPECIFICATIONS
Sample
volume
for a 5mm
thick gel
Code
Description
MS15-1-0.75
Comb Prep 1, Marker 1, 0.75mm thick
371µl
MS15-2-0.75
Comb Prep 2, Marker 2, 0.75mm thick
169µl
MS15-4-0.75
Comb Prep 4, Marker 2, 0.75mm thick
91µl
MS15-10-0.75
Comb 10 sample, 0.75mm thick
34µl
MS15-12-0.75
Comb 12 sample, 0.75mm thick
30µl
MS15-20-0.75
Comb 20 sample, 0.75mm thick
16µl
MS15-35-0.75
Comb 35 sample, 0.75mm thick
7µl
MS15-10MC-0.75
Comb 10 sample MC, 0.75mm thick
22µl
MS15-14MC-0.75
Comb 14 sample MC, 0.75mm thick
22µl
MS15-16MC-0.75
Comb 16 sample MC, 0.75mm thick
20µl
MS15-18MC-0.75
Comb 18 sample MC, 0.75mm thick
8µl
MS15-28MC-0.75
Comb 28 sample MC, 0.75mm thick
8µl
MS15-30MC-0.75
Comb 30 sample MC, 0.75mm thick
9µl
MS15-1-1
Comb Prep 1, Marker 1, 1mm thick
495µl
MS15-2-1
Comb Prep 2, Marker 2, 1mm thick
225µl
MS15-4-1
Comb Prep 4, Marker 2, 1mm thick
122µl
MS15-10-1
Comb 10 sample, 1mm thick
45µl
MS15-12-1
Comb 12 sample, 1mm thick
41µl
Gel dimensions
(w x l)
15 x 7cm 15 x 10cm
15 x 15cm 15 x 20cm 15 x 25cm
MS15-20-1
Comb 20 sample, 1mm thick
21µl
Unit dimensions
(w x l x h)
MS15-35-1
Comb 35 sample, 1mm thick
10µl
17.5 x 26.5 x 9cm
17.5 x 41 x 9cm (Stretch models only)
MS15-10MC-1
Comb 10 sample MC, 1mm thick
29µl
MS15-14MC-1
Comb 14 sample MC, 1mm thick
29µl
MS15-16MC-1
Comb 16 sample MC, 1mm thick
27µl
Max. sample
capacity
15 x 7cm tray – 70 samples
15 x 10cm tray – 140 samples
15 x 15cm tray – 210 samples
15 x 20cm tray – 280 samples
15 x 25cm tray – 350 samples
MS15-18MC-1
Comb 18 sample MC, 1mm thick
11µl
MS15-28MC-1
Comb 28 sample MC, 1mm thick
11µl
MS15-30MC-1
Comb 30 sample MC, 1mm thick
13µl
MS15-1-1.5
Comb Prep 1, Marker 1, 1.5mm thick
743µl
MS15-2-1.5
Comb Prep 2, Marker 2, 1.5mm thick
338µl
MS15-4-1.5
Comb Prep 4, Marker 2, 1.5mm thick
182µl
MS15-10-1.5
Comb 10 sample, 1.5mm thick
68µl
MS15-12-1.5
Comb 12 sample, 1.5mm thick
61µl
MS15-20-1.5
Comb 20 sample, 1.5mm thick
32µl
MS15-35-1.5
Comb 35 sample, 1.5mm thick
15µl
MS15-10MC-1.5
Comb 10 sample MC, 1.5mm thick
44µl
MS15-14MC-1.5
Comb 14 sample MC, 1.5mm thick
44µl
MS15-16MC-1.5
Comb 16 sample MC, 1.5mm thick
41µl
MS15-18MC-1.5
Comb 18 sample MC, 1.5mm thick
17µl
MS15-28MC-1.5
Comb 28 sample MC, 1.5mm thick
17µl
MS15-30MC-1.5
Comb 30 sample MC, 1.5mm thick
19µl
MS15-1-2
Comb Prep 1, Marker 1, 2mm thick
990µl
MS15-2-2
Comb Prep 2, Marker 2, 2mm thick
450µl
MS15-4-2
Comb Prep 4, Marker 2, 2mm thick
243µl
MS15-10-2
Comb 10 sample, 2mm thick
90µl
MS15-12-2
Comb 12 sample, 2mm thick
81µl
MS15-20-2
Comb 20 sample, 2mm thick
43µl
MS15-35-2
Comb 35 sample, 2mm thick
20µl
MS15-10MC-2
Comb 10 sample MC, 2mm thick
59µl
Buffer volume
500ml (choice) 1000ml (choice stretch)
Combs available:
No. of samples
Thicknesses
1, 2, 4, 10, 10MC, 12, 14MC, 16,
18MC, 20, 28MC, 30MC, 35
0.75, 1, 1.5, 2mm
MultiSUB™ Choice ACCESSORIES
MS15-UV7
15 x 7cm UV Tay
MS15-UVDAM
Casting Dams
MS15-UV10
15 x 10cm UV Tray
MS15-PE
Positive Electrode
MS15-UV15
15 x 15cm UV Tray
MS15-NE
Negative Electrode
MS15-14MC-2
Comb 14 sample MC, 2mm thick
59µl
Comb 16 sample MC, 2mm thick
54µl
MS15-UVST20
15 x 20 UV Tray
MS15-LG
Adhesive Loading Guides
MS15-16MC-2
MS15-UVST25
15 x 25 cm V Tray
MS15-WP
Viewing Platform
MS15-18MC-2
Comb 18 sample MC, 2mm thick
23µl
MS15-UVS
15cm UV Gel Scoop
MSCHOICECP
Cool-Pack and Platform
MS15-28MC-2
Comb 28 sample MC, 2mm thick
23µl
MS15/20-FC
multiSUB™ Choice/Maxi Flexi caster
MS15-30MC-2
Comb 30 sample MC, 2mm thick
25µl
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features:
• Three tray options
• Run up to 210
samples
• Low buffer volumes
• Multichannel
pipette compatible
combs for speed
loading
multiSUB™ Choice
The multiSUB™ Choice offers a wide
degree of versatility. Three tray options
are available – 15 x 7cm, 15 x 10cm
and 15 x 15cm – allowing the choice
of one, two or all three gel length
options at the time of purchase.
Further purchases of additional
accessories are no longer required.
Maximising comb and tray options
allow up to 210 samples to be resolved
per gel. The 15cm total run length
allows restriction fragment or other
close MW sample bands to be easily
separated and identified. Speed loading
is accomplished using 10, 14, 16, 18,
28 or 30 sample multi-channel pipette
compatible combs. multiSUB™ choice
stretch units are available with optional
15 x 20cm and 15 x 25cm gel trays
and four 28-sample combs for those
researchers wanting to perform higher
resolution separation of more samples
over a longer distance. multiSUB™
Choice Trio includes all 3 tray sizes for
optimum versatility and value.
Condensation free lids available
multisub™ Choice stretch version
Typical Applications
Ideal for restriction fragment analysis,
sample prep or checking of high
numbers of samples
14
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 15
MultiSUB™ MAXI COMBS
ORDERING INFORMATION
Sample
volume
for a 5mm
thick gel
MSMAXI10
multiSUB Maxi
20 x 10cm UV Tray, 2 x 20 sample, 1mm thick
combs, casting dams, loading guides
Code
Description
MSMAXI15
multiSUB Maxi
20 x 15cm UV Tray, 2 x 20 sample, 1mm thick
combs, casting dams, loading guides
MS20-1-0.75
Comb Prep 1, Marker 1, 0.75mm
thick
506µl
MSMAXI20
multiSUB Maxi
20 x 20cm UV Tray, 2 x 20 sample, 1mm thick
combs, casting dams, loading guides
MS20-2-0.75
Comb Prep 2, Marker 2, 0.75mm
thick
236µl
MSMAXIDUO
multiSUB Maxi
20 x 10 & 20 x 20cm UV Tray, 2 x 20 sample,
1mm thick combs, casting dams, loading guides
MS20-4-0.75
Comb Prep 4, Marker 2, 0.75mm
thick
115µl
MS20-10-0.75
Comb 10 sample, 0.75mm thick
54µl
MSMAXI25
multiSUB Maxi
20 x 25cm UV Tray, 2 x 20 sample, 1mm thick
combs, casting dams, loading guides
MS20-16-0.75
Comb 16 sample, 0.75mm thick
30µl
MS20-20MC-0.75
Comb 20 sample MC, 0.75mm thick
20µl
MS20-UV10
20 x 10cm UV Tray
MS20-25-0.75
Comb 25 sample, 0.75mm thick
16µl
MS20-UV15
20 x 15cm UV Tray
MS20-30-0.75
Comb 30 sample, 0.75mm thick
13µl
MS20-UV20
20 x 20cm UV Tray
MS20-36-0.75
Comb 36 sample, 0.75mm thick
11µl
MS20-UV25
20 x 25cm UV Tray
MS20-40MC-0.75
Comb 40 sample MC, 0.75mm thick
8µl
MS20-UVDAM
Casting Dams
MS20-50-0.75
Comb 50 sample, 0.75mm thick
8µl
MS15/20-FC
multiSUB Choice/Maxi Flexi caster
MS20-1-1
Comb Prep 1, Marker 1, 1mm thick
675µl
MS20-LG
Adhesive Loading Guides
MS20-2-1
Comb Prep 2, Marker 2, 1mm thick
315µl
MS20-WP
Viewing Platform
MS20-4-1
Comb Prep 4, Marker 2, 1mm thick
153µl
MS20-PE
Positive Electrode
MS20-10-1
Comb 10 sample, 1mm thick
72µl
MS20-NE
Negative Electrode
MS20-16-1
Comb 16 sample, 1mm thick
41µl
MS20-UVS
20cm UV Gel Scoop
MS20-20MC-1
Comb 20 sample MC, 1mm thick
27µl
MSMAXICP
Cool-Pack and Platform
MS20-5-1
Comb 25 sample, 1mm thick
21µl
MSMAXIBSB
Buffer Saver Blocks pk/2 saves 450ml of buffer
MS20-30-1
Comb 30 sample, 1mm thick
17µl
MS20-36-1
Comb 36 sample, 1mm thick
14µl
MS20-40MC-1
Comb 40 sample MC, 1mm thick
11µl
MS20-50-1
Comb 50 sample, 1mm thick
10µl
MS20-1-1.5
Comb Prep 1, Marker 1, 1.5mm thick
1013µl
MS20-2-1.5
Comb Prep 2, Marker 2, 1.5mm thick
473µl
MS20-4-1.5
Comb Prep 4, Marker 2, 1.5mm
thick
230µl
MS20-10-1.5
Comb 10 sample, 1.5mm thick
108µl
MS20-16-1.5
Comb 16 sample, 1.5mm thick
61µl
MS20-20MC-1.5
Comb 20 sample MC, 1.5mm thick
41µl
MS20-25-1.5
Comb 25 sample, 1.5mm thick
32µl
MS20-30-1.5
Comb 30 sample, 1.5mm thick
26µl
MS20-36-1.5
Comb 36 sample, 1.5mm thick
22µl
MS20-40MC-1.5
Comb 40 sample MC, 1.5mm thick
17µl
MS20-50-1.5
Comb 50 sample, 1.5mm thick
16µl
MS20-1-2
Comb Prep 1, Marker 1, 2mm thick
1350µl
MS20-2-2
Comb Prep 2, Marker 2, 2mm thick
630µl
MS20-4-2
Comb Prep 4, Marker 2, 2mm thick
306µl
MS20-10-2
Comb 10 sample, 2mm thick
144µl
MS2016-2
Comb 16 sample, 2mm thick
81µl
MS20-20MC-2
Comb 20 sample MC, 2mm thick
54µl
MS20-25-2
Comb 25 sample, 2mm thick
42µl
MS20-30-2
Comb 30 sample, 2mm thick
34µl
MS20-36-2
Comb 36 sample, 2mm thick
29µl
MS20-40MC-2
Comb 40 sample MC, 2mm thick
23µl
Typical Applications
MS20-50-2
Comb 50 sample, 2mm thick
21µl
Primarily designed for separating high
numbers of samples from PCR or
cloning
MultiSUB Maxi complete system
TECHNICAL SPECIFICATIONS
Gel dimensions
(w x l)
20 x 10cm
20 x 15cm
20 x 20cm
20 x 25cm
Unit dimensions
(w x l x h)
23 x 39.5 x 9cm
Max Sample
Capacity
20 x 10cm tray - 200 samples
20 x 15cm tray - 350 samples
20 x 20cm tray - 450 samples
20 x 25cm tray - 550 samples
Buffer volume
1500ml
Combs available:
No. of samples
Thicknesses
1, 2, 4, 10, 16, 20MC, 25, 30, 36, 40MC, 50
0.75, 1, 1.5, 2mm
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features:
• Now available with
20 x 25cm, 20 x
20cm, 20 x 15cm or 20 x 10cm gel trays
• Run up to 550
samples
• Low buffer volumes
• Ideal for extended
separations
multiSUB™ Maxi
The multiSUB™ Maxi is primarily
designed for resolution of high
numbers of samples such as from
Cloning or PCR. The multiSUB™ Maxi
allows ultra high-resolution separations
over extended runs. Tray sizes
correspond to standard blotter sizes.
It also allows easy sample transfer onto
a membrane for further analysis.
compatible combs up to 40 sample
facilitate speed loading of up to 440
samples per gel. 50 sample combs
allow maximum sample capacity of 550
samples per gel.Casting dams allow
gels to be rapidly cast externally while
the multiSub™ unit is in use for
gel running.
Four gel tray sizes are available –
20 x 10cm, 20 x 15cm, 20 x 20cm
and 20 x 25cm. Multichannel pipette
Condensation free lids available
multiSUB™ Choice/Maxi flexi caster
16
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 17
MultiSUB™ SCREEN ACCESSORIES
MS26-UV32
26 x 32cm, UV Tray
MS26-PE
Positive Electrode
MS26-UV24
26 x 24cm, UV Tray
MS26-NE
Negative Electrode
MS26-UV16
26 x 16cm, UV Tray
MS26-WP
Viewing Platform
MS26-LG
Adhesive Loading
Guides
MSSCRNBSB MS26-FC
Flexicaster for gels
up to 32cm. Casts
MSMINI, MSMIDI,
MSCHOICE,
MSMAXI and
MSSCREEN gels.
MS26-UVS
26cm UV Gel Scoop
Buffer Saver
Blocks, pk/2 saves
625ml of buffer
MS26-x -yLG
CombiComb and
Loading Guide;
x = comb well
no.,y = comb
Thickness
MSSCRNCP
Cool-Pack and
Platform
MultiSUB™ SCREEN combs
Code
Description
a 5mm thick gel
Sample
volume
for a 5mm
thick gel
MS26-28MC-0.75
Comb 28 sample MC, 0.75mm thick
25µl
MS26-56MC-0.75
Comb 56 sample MC, 0.75mm thick
10µl
MS26-28MC-1
Comb 28 sample MC, 1mm thick
34µl
MS26-56MC-1
Comb 56 sample MC, 1mm thick
14µl
MS26-28MC-1.5
Comb 28 sample MC, 1.5mm thick
51µl
MS26-56MC-1.5
Comb 56 sample MC, 1.5mm thick
20µl
MS26-28MC-2
Comb 28 sample MC, 2mm thick
68µl
MS26-56MC-2
Comb 56 sample MC, 2mm thick
27µl
ORDERING INFORMATION
MSSCREEN32
multiSUB Screen, 26 x 32cm UV Tray, 6 x 28
sample combs, loading guides & flexicaster
MSSCREEN24
multiSUB Screen, 26 x 24cm UV Tray, 6 x 28
sample combs, loading guides & flexicaster
MSSCREEN16
multiSUB Screen, 26 x 16cm UV Tray, 6 x 28
sample combs, loading guides & flexicaster
MSSCREENTRIO
multiSUB Screen, 26 x 16cm, 26 x 24cm, 26 x
32cm UV Trays, 6 x 28 sample combs, loading
guides & flexicaster
Technical Specifications
Gel dimensions (w x l)
26 x 32cm, 26 x 24cm, 26 x 16cm
Unit dimensions (w x l x h)
28 x 50 x 9cm
Max. sample
capacity
26x32cm tray – 672 samples
26x24cm tray – 504 samples
26x16cm tray – 336 samples
Buffer volume
2 litres
Combs available:
No. of samples
Thicknesses
28MC, 56MC
0.75, 1, 1.5, 2mm
features:
• Available with 26 x
16, 26 x 24 and 26
x 32cm, or all three
gel trays
• Run up to 672
samples
• Buffer Recirculation
Included
• Multichannel
pipette compatible
combs for speed
loading
multiSUB™ Screen
Designed for rapid screening of very large numbers of Clonal or PCR samples, the
multiSUB™ Screen horizontal gel unit has a 672 maximum sample capacity per gel.
This allows loading and analysis of exactly seven 96-well format microtitre plates.
The large gel run length of 32cm also allows resolution of samples over a long
distance for separation of complex sample bands such as in restriction fragment
analysis.
The unit is available with a full length tray or with other tray length options of
16 or 24cm so that the user’s exact requirements can be matched. In addition
to options for single length gel trays, the multiSUB™ Screen is available with all
three gel tray lengths to provide the maximum in flexibility, versatility and value.
Buffer recirculation ports are included as standard to allow enhanced resolution
over extended runs while loading guides enhance well visibility for easy sample
loading.
The MS26-FC flexicaster is now included with each multiSUB™ screen unit.
Typical Applications
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Ideal for checking very high number of
samples for pcr and cloning.
Msscreen with recirculation ports
Flexi caster for all gels upto 32cm
18
I HORIZONTAL GEL SYSTEMS
miniRAPIDE
The miniRapide is a completely self contained system
designed for quick checks of samples. Gel casting, running
and analysis are all performed in the same ultra compact
unit. Buffer and gel volumes have been kept to a minimum
and the parallel electrode arrangement allows ultra efficient
current transfer, enabling resolution to be completed within
30 minutes. The UV transparent base allows direct viewing
on a UV Transilluminator with no need for time consuming
transfer and potential gel damage. Dual comb slots allow
the loading of up to 40 samples per gel while multichannel
pipette compatible combs further enhance the speed and
convenience.
ORDERING INFORMATION
FMMS10
miniRapide 10 x 8cm, 2 x 8 sample combs
1.5mm thick and casting dams
FMMS-DAM
miniRapide Casting dams, pk/2
RPW0.2
Replacement Platinum Wire 0.2mm – 50cm
TECHNICAL SPECIFICATION
Gel dimensions (w x l)
Unit dimensions (w x l x h)
Max. samplecapacity
Buffer volume
Combs available:
No. of samples
Thicknesses
10 x 8cm
15 x 15 x 4cm
40 samples
50ml
1, 4, 8, 12, 16, 20
1, 1.5mm
Typical Applications
For routine, rapid checking of samples
HORIZONTAL GEL SYSTEMS I 19
features:
• A convenient self contained solution for
routine agarose gel electrophoresis
features:
• Ultra compact
• Low buffer volumes
• Multichannel pipette compatible combs for
speed loading
• Low voltage makes the MSBGEL ideal for
use in schools and colleges where safety is
paramount
• Integrated safety lid makes the gel
inaccessible during use
• UV-transparent acrylic for direct visualisation
of the gel within the tank
MIN RAPIDE combs
Sample
volume
for a 5mm
thick gel
Code
Description
FMMS-1-1
Comb 1 sample , 1 dye , 1mm thick
330µl
FMMS-4-1
Comb 4 sample, 1mm thick
90µl
FMMS-8-1
Comb 8 sample, 1mm thick
40µl
FMMS-12-1
Comb 12 sample, 1mm thick
25µl
FMMS-16-1
Comb 16 sample, 1mm thick
15µl
FMMS-20MC-1
Comb 20 sample MC, 1mm thick
10µl
FMMS-1-1.5
Comb 1 sample , 1 dye , 1.5mm thick
495µl
FMMS-4-1.5
Comb 4 sample, 1.5mm thick
135µl
FMMS-8-1.5
Comb 8 sample, 1.5mm thick
60µl
FMMS-12-1.5
Comb 12 sample, 1.5mm thick
38µl
FMMS-16-1.5
Comb 16 sample, 1.5mm thick
23µl
FMMS-20MC-1.5
Comb 20 sample MC, 1.5mm
thick
15µl
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FOR MORE INFORMATION
• Small size minimises buffer and gel
consumption
• Cost-effective – no need for expensive power
supplies, or transilluminators if supplied with
optional kit
Technical Specifications
Gel dimensions (w x l)
6 x 7.5cm
Unit dimensions (w x l x h)
17 x 11 x 3.5cm
Max. sample (capacity)
32 samples
Buffer volume
Combs available:
No. of samples
Thicknesses
Battery Gel
The MSBGEL battery mini-gel is designed specifically with
safety and cost in mind: as no power supply is necessary.
Results are obtainable within 1.5 hours and it is ideal for
schools, classrooms and electrophoresis in the field where no
power source is available.
ORDERING INFORMATION
MSBGEL
Battery operated horizontal gel 6
x 7.5cm, 2 x 8 sample 1-mm-thick
combs, casting gates
RPW0.2
Replacement Platinum Wire
0.2mm – 50cm
MSBGEL-CG
Casting gates
Pk/2 for MSBGEL
MSBG-1-8
Comb 8 sample,
1mm thick
50ml
MSBGEL-BAT
PP3 batteries
for MSBGEL
Pk/2
MSBG-1-12
Comb 12 sample, 1mm thick
4, 8, 12 and 16
1mm
MSBG-1-4
Comb 4 sample,
MSBG-1-16
1mm thick
Comb 16 sample, 1mm thick
Typical Applications
Teaching and field electrophoresis
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FOR MORE INFORMATION
20
I HORIZONTAL GEL SYSTEMS
ORDERING INFORMATION
MSMIDI96
multiSUB™ Midi96, UV tray, Comb block with
12 x 8 sample, 1mm thick combs, casting dams
MSMIDI961.5
multiSUB™ Midi96, UV tray, Comb block with
12 x 8 sample, 1.5mm thick combs, casting
dams. Combs have one marker lane
MSMIDI96/2M
multiSUB™ Midi96, UV tray, Comb block with
12 x 8 sample, 1mm thick combs, casting dams.
Combs have two marker lanes
multiSUB™ Midi96, UV tray, Comb block with
MSMIDI96/1.5/2M 12 x 8 sample, 1.5mm thick combs,
casting dams. Combs have two marker lanes
MSMIDI96ST
multiSUB™ Midi96 STRETCH, UV tray, Comb
block with 12 x 8 sample, 1mm thick combs,
casting dams
MSMIDI96ST1.5
multiSUB™ Midi96 STRETCH, UV tray, Comb
block with 12 x 8 sample, 1.5mm thick combs,
casting dams. Combs have one marker lane
MSMIDI96ST/2M
multiSUB™ Midi96 STRETCH, UV tray, Comb
block with 12 x 8 sample, 1mm thick combs,
casting dams. Combs have two marker lanes
MSMIDI96ST
/1.5/2M
multiSUB™ Midi96 STRETCH, UV tray , Comb
block with 12 x 8 sample, 1.5mm thick combs, casting dams. Combs have two marker
lanes
MSMIDI96STDBL
multiSUB™ Midi96 STRETCH, UV tray, Comb
block with 24 x 8 sample, 1mm thick combs,
casting dams - N.B. run length = 1.8cm.
MSMIDI96-8-1-CB
MSMIDI 96 Comb 8 sample MC + 1 Marker,
1mm thick COMB BLOCK
MSMIDI96-8-1.5CB
MSMIDI 96 Comb 8 sample MC + 1 Marker,
1.5mm thick COMB BLOCK
MSMIDI96-81/2M-CB
MSMIDI 96 Comb 8 sample MC + 2 Marker,
1mm thick COMB BLOCK
MSMIDI96-81.5/2M-CB
MSMIDI 96 Comb 8 sample MC + 2 Marker,
1.5mm thick COMB BLOCK
MSMIDI96ST-81-CB
MSMIDI 96 STRETCH Comb 8 sample MC + 1
Marker, 1mm thick COMB BLOCK
MSMIDI96ST-81.5-CB
MSMIDI 96 STRETCH Comb 8 sample MC + 1
Marker, 1.5mm thick COMB BLOCK
MSMIDI96ST-81/2M-CB
MSMIDI 96 STRETCH Comb 8 sample MC + 2
Marker, 1mm thick COMB BLOCK
MSMIDI96ST-81.5/2M-CB
MSMIDI 96 STRETCH Comb 8 sample MC + 2
Marker, 1.5mm thick COMB BLOCK
MSMIDI96-8-1
MSMIDI 96 Comb 8 sample MC, 1mm thick.
one marker lane.
MSMIDI96-8-1.5
MSMIDI 96 Comb 8 sample MC, 1.5mm thick.
one marker lane.
MSMIDI96-8-1/2M
MSMIDI 96 Comb 8 sample MC, 1mm thick.
two marker lanes.
MSMIDI96-8-1.5/2M
MSMIDI 96 Comb 8 sample MC, 1.5mm thick.
two marker lanes
MS10-UV96
multiSUB™ Midi, 96 Well Tray
MS10-UV96ST
multiSUB™ Midi STRETCH, 96 Well Tray
HORIZONTAL GEL SYSTEMS I 21
TECHNICAL SPECIFICATIONS
Gel dimensions (w x l)
msmidi96:
msmidi96st:
10 x 12cm
10 x 24cm
Unit dimensions (w x l x h)
msmidi96:
msmidi96st:
12.5 x 22 x 9cm
12.5 x 46.5 x 8cm
Max. sample
capacity
96 samples plus
1 or 2 marker lanes
Buffer volume (w x l)
msmidi96:
msmidi96st:
300ml
700ml
Combs available:
No. of samples
Thicknesses
8 + 1x Marker,
8 + 2x Markers
1, 1.5mm
Multisub Midi 96 Stretch
features:
• Designed for
loading of DNA samples from multiwell plates
• Ideal for high
throughput
electrophoresis
• A
verage run-time
is just 15 to 30
minutes
• Direct microplate
format for easy lane
identification
• Multichannel
pipette compatible
combs for speed
loading
multiSUB™ Midi 96
The multiSUB™ Midi 96 Gel
System is ideal for high throughput
electrophoresis of PCR products or
samples containing a small number of
DNA bands.
Its 10x12cm (W x L) gel dimensions
and 96-well comb block format
correspond to the standard microplate
configuration. Two different types of
comb are available: one which has 8
wells and one additional lane for DNA
markers and one which has 8 wells
and 2 additional lanes for markers.
The offset layout of the wells allows
a maximum run length of 1.8cm per
well, resulting in improved separation.
multiSUB™ Midi 96 can accommodate
samples from a 96-well plate – samples
can be loaded directly using 8-channel
pipettes. As with all multiSUB™ units,
leak-proof casting is simplified using
the supplied casting dams while their
ultra-compact size minimises buffer
usage and bench space requirements.
MSMIDI96ST Stretch Systems are also
available for those users requiring an
extended run length per well of up to
3.6cm, or for loading of samples from
two 96-well plates - MSMIDI96STDBL.
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Ideal for analysis of up to 96 PCRfragment length polymorphisms loaded
from 96-well microplates or thermal
cycler blocks
multisub™ Midi 96 - Well comb blocks
multisub™ Midi 96 - Complete system
22
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 23
features:
• Separates a maximum 1200 samples in as little
as 15 minutes in 4 stacked horizontal gel trays
• Multichannel-compatible combs and gel plate
configurations compatible with microplates
and thermal cycler blocks ensure rapid loading
of DNA minipreps and PCR products by
8-channel pipette
• Double-sided 1.5-mm-thick combs allow more
sample volume to be loaded into each well
• Three gel tray options available in 8x6,
8x12 and 8x18cm (WxL) sizes for maximum
flexibility
features:
• Small footprint area reduces uptake of bench
space within the laboratory
• Adjustable barrier with ultra-soft silicone
gasket ensures leak-proof casting
• Mini flexicaster accommodates 7x7, 7x10,
10x7 and 10x10cm gel trays
ORDERING INFORMATION
CSL-MULTISUB4
CSL-MULTISUB4EXCAS
multiSUB™-4
The multiSUB™-4 is a compact system capable of running a
maximum 1200 samples simultaneously by stacking up to 4
horizontal gels. This achieves high sample throughput while
maintaining the time- and space-saving advantages of a
small, compact unit.
Each multiSUB™-4 is supplied with 4 gel trays and 8 combs
as standard, while additional trays and combs may be
purchased as required. Two double-sided comb and three
tray length formats are also available.
An optional flexicaster, either supplied separately or as a
package with the multiSUB™-4, is also available that allows
up to four 8x6, 8x12 and 8x18cm multiSUB™-4 gel trays to
be cast simultaneously outside the tank.
Typical Applications
Ideal for rapid checking of very large numbers of samples
from PCR and cloning. High throughput Electrophoresis.
multisub4, Multi-Level Gel Chamber,
Includes 4x 18cm UV Trays, 8x 18/8 Sample
1.5mm Combs (Tape UV Trays To Seal)
multisub4, As Above But With External
Caster For 4 Gels
MSUB4UV6
multiSUB4 tray 8 x 6cm
MSUB4UV12
multiSUB4 tray 8 x 12cm
MSUB4UV18
multiSUB4 tray 8 x 18cm
MSUB4EXCASTER
External Caster For Msub4 (4 Trays)
MSUB4-12/1-1
12/1 Sample 1mm Combs for multiSUB4
MSUB4-18/8-1
18/8 Sample 1mm Combs for multiSUB4
MSUB4-12/1-1.5
12/1 SAMPLE 1.5mm Combs for multiSUB4
MSUB4-18/8-1.5
18/8 SAMPLE 1.5mm Combs for multiSUB4
CSL-CAB
Electrophoresis cable (Black & Red)
TECHNICAL SPECIFICATION
• Maxi flexicaster accommodates 15x7, 15x10
and 15x15 and 20x10, 20x15, 20x20 and
20x25cm gel trays
• Maxi flexicaster also accommodates MSMINI
and MSMIDI gel trays
• MS26 flexicaster accommodates all tray sizes
(gel can be poured at 80 degree C)
ORDERING INFORMATION
MS7/10-FC
multiSUB Mini/Midi Flexi Caster
MS15/20-FC
multiSUB Choice/Maxi Flexi Caster
MS26-FC
multiSUB SCREEN Flexi caster
MS26-FC Tray Compatibility
Gel dimensions (w x l)
8 x 6cm, 8 x 12cm, 8 x 18cm
Unit dimensions (w x l x h)
11 x 35 x 16cm
Tray
Caster Capacity
per 18cm tray
with 1cm run length: 306
with 2cm run length: 144
with 3cm run length: 72
200, 400, 600 or 800ml
(for 1, 2, 3 or 4 gel trays resp.)
MS7-UV7, MS7-UV10.
3 of each
MS10-UV7, MS10-UV10. MS15-UV7,
MS15-UV10, MS15-UV15, MS15-UV17,
2 of each
MS15-UVST20, MS15-UVST25.
MS20-UV10, MS15-UV15,
1 of each
1, 8, 12, 18 DuoCombs
1, 1.5mm
MS20-UV17, MS15-UV20, MS15-UV25.
1 of each
MS26-UV16, MS26-UV24, MS26-UV32.
1 of each
Max. sample
capacity
Buffer volume
Combs available:
No. of samples
Thicknesses
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Typical Applications
Casting of horizontal gels of multiple lengths
Casting Systems
Three horizontal flexicasters are now available for use with
the multiSUB™ Mini, Midi, Choice, Maxi and MSSCREEN
gel trays. Each flexicaster is entirely versatile and when
casting, one end of the gel tray is pushed into position
against a fixed wall, while a moveable dam with twin
cam-lock design and rubber insert locks into position at the
other end to provide a leak proof seal. No further casting
accessories are needed other than the gel tray, while
adjustable levelling feet and a spirit bubble facilitate even
gel-pouring.
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
24
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 25
features:
• View you gel more
easily.
• Removes some of the
heat generated by
the electrophoresis
procedure.
• Unique feature to the
multiSUB™ range of
tanks.
Accessories
Ordering Information
New Cleaver
Scientific
ClearSight
Electrophoresis
Complete systems:-
A common problem associated with electrophoresis is
condensation build up on the lids which obscures the
progress of the run. This can make it difficult to see the dye
progression in the gel and thus difficult to work out how far
the samples have actually run.
MSMINI7CS
ClearSight MSMINI7, Fan & power source.
MSMINI10CS
ClearSight MSMINI10, Fan & power source
MSMINIDUOCS
ClearSight MSMINIDUO, Fan & power source.
MSMIDI7CS
ClearSight MSMINI7, Fan & power source.
MSMIDI10CS
ClearSight MSMIDI10, Fan & power source.
MSMIDIDUOCS
ClearSight MSMIDIDUO, Fan & power source.
MSCHOICE7CS
ClearSight MSCHOICE7, Fan & power source.
MSMINICP
Cool-Pack and Platform for MSMINI
MSCHOICE10CS
ClearSight MSCHOICE10, Fan & power source.
MSMIDICP
Cool-Pack and Platform for MSMIDI
MSCHOICE15CS
ClearSight MSCHOICE15, Fan & power source.
MSCHOICECP
Cool-Pack and Platform for MSCHOICE
MSCHOICETRIOCS
ClearSight MSCHOICETRIO, Fan & power source.
MSMAXICP
Cool-Pack and Platform for MSMAXI
MSCHOICETRIO15CS
ClearSight MSCHOICETRIO15, Fan & power
source.
MSSCREENCP
Cool-Pack and Platform for MSSCREEN
It is often the case that the gel is stopped and checked by
removing the lid which is time consuming.
MSCHOICEST20CS
ClearSight MSCHOICEST20, Fan & power source.
MSCHOICEST25CS
ClearSight MSCHOICEST25, Fan & power source.
Cleaver Scientific ClearSight lids solve this problem.
MSMAXIDUOCS
ClearSight MSMAXIDUO, Fan & power source.
These completely remove any condensation build up and
give a perfectly clear view of the gel and the dye lane
progression during the run using a USB powered fan within
the lid. These are available either purchased as complete
systems or if you already own a Cleaver Scientific gel tank as
an upgrade.
MSMIDI96CS
ClearSight MSMIDI96, Fan & power source.
Simply plug the lid into the USB source that is supplied and
experience perfect clearly visible gels every time.
System Upgrades:-
The Gel Levelling Table provides a useful way of pouring
gels of uniform thickness. The table features a large 32 x
26cm platform with large thumb-wheel levelling feet at each
corner. A levelling bubble is supplied.
Buffer Saver Blocks
Ordering Information
High Efficiency Horizontal Gel Cooling Systems
Horizontal Buffer Saver Blocks
MSMINIBSB
Buffer Saver Blocks for MSMINI, pk/2 saves 100ml
of buffer
MSMIDIBSB
Buffer Saver Blocks for MSMIDI, pk/2 saves 100ml
of buffer
MSCHOICEBSB
Buffer Saver Blocks for MSCHOICE, pk/2 saves
190ml of buffer
MSMAXIBSB
Buffer Saver Blocks for MSMAXI, pk/2 saves 450ml
of buffer
MSSCREENBSB
Buffer Saver Blocks for MSSCREEN, pk/2 saves
625ml of buffer
CLEARSIGHTMINI
ClearSight Lid for multiSub Mini, Fan & power
source.
CLEARSIGHTMIDI
ClearSight Lid for multiSub Midi, Fan & power
source.
CLEARSIGHTCHOICE
ClearSight Lid for multiSub Choice, Fan & power
source.
UV Fluorescent Ruler
CLEARSIGHTMAXI
ClearSight Lid for multiSub Maxi, Fan & power
source.
CSL-RULER
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
MSMIDI-FL
Gel Levelling Table
Gel Levelling Table
CSL-GLT Gel Levelling Table
UV Fluorescent Ruler
Buffer saver blocks physically reduce the volume in positive
and negative compartments of a gel chamber and so reduces
buffer requirements, saving cost. Blocks are available for all
multiSUB™ Horizontal Gel Units.
RunFAST High Efficiency
Horizontal Gel Cooling
• Effective cooling
• Cools without external chilling systems
• Improves gel resolution and reduces run times
Comprising a removable Gel Platform and CoolPack the
unique runFAST system will cool the buffer chamber of
multiSUB™ horizontal gel units without any external chilling.
Simply place the platform over the running tray and place
a pre-chilled CoolPack on top. This allows the CoolPack
to sit above the gel in buffer directly removing any heat
generated.
Gel Rulers
• Sharp, clear, easily visible markings
• For use with ethidium bromide gels
• Ideal for gel presentation
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
26
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 27
Package Deals Horizontals
Maxi Horizontal
with Power Supply
Cleaver Scientific now offers a comprehensive range of Combination Packages to
address any budget or horizontal electrophoresis application.
Ordering Information
MSMAXIDUO-CS300 Maxi Horizontal Electrophoresis Package:
MSMAXIDUO
horizontal unit with 20x10cm
and 20x20cm gel trays and
combs (Pg 15)
CS-300V
300V, 700mA, 150W midi
power supply (Pg 87)
MSMAXIDUO-CS300KIT M
axi Horizontal Electrophoresis Package with electrophoresis
starter kit, includes:
Mini Horizontal
with Power Supply
MSMAXIDUO-CS300
Ordering Information
CSL-LOADDYE
MSMINIDUO-NANO300 Mini Horizontal Electrophoresis Package, includes:
MSMINIDUO
horizontal unit with 7x7cm and
7x10cm gel trays and combs
nanoPAC-300
(Pg 9)
MSMINIDUO-NANO300
CSL-LOADDYE
10x Bromophenol Blue Loading
CSL-AG100
Dye, 1ml (Pg 140)
CSL-MDNA-1kb 1kb DNA ladder (Pg 139)
Midi Horizontal
with Power Supply
CSL-TBEP
100g agarose (Pg 136)
Screen Horizontal
with Power Supply
MSSCREENTRIO-CS500 Screen Horizontal Electrophoresis Package, includes:
nanoPAC-300
10x Bromophenol Blue Loading
CSL-AG100
Dye, 1ml (Pg 140)
CSL-TBEP
100g agarose (Pg 136)
Midi96 Horizontal
with Power Supply
300V, 700mA, 150W mini
power supply (Page 87)
MSMIDI96-CS300KIT M
idi96 Horizontal Electrophoresis Package with electrophoresis starter
kit, includes:
CSL-LOADDYE
CS-300V
10x Bromophenol Blue Loading
CSL-AG100
Dye, 1ml (Pg 140)
CSL-TBEP
100g agarose (Pg 136)
Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches
(Pg 138)
10x Bromophenol Blue Loading
CSL-AG100
Dye, 1ml (Pg 140)
CSL-MDNA-1kb 1kb DNA ladder (Pg 139)
300V, 700mA, 150W midi
power supply (Pg 87)
MSCHOICETRIO-CS300
CSL-MDNA-1kb 1kb DNA ladder (Pg 139)
horizontal unit with UV tray and
12x8-sample 1mm comb block CS-300V
(Page 21)
MSMIDI96-CS300
MSCHOICETRIO-CS300KIT Choice Horizontal Electrophoresis Package with electrophoresis
starter kit, includes:
CSL-LOADDYE
Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches
(Pg 138)
MSMIDI96-CS300 Midi96 Horizontal Electrophoresis Package, includes:
MSMIDI96
Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches
(Pg 138)
CSL-TBEP
100g agarose (Pg 136)
Ordering Information
MSCHOICETRIO-CS300 Choice Horizontal Electrophoresis Package, includes:
MSCHOICE
TRIO
10x Bromophenol Blue Loading
CSL-AG100
Dye, 1ml (Pg 140)
CSL-MDNA-1kb 1kb DNA ladder (Pg 139)
300V, 400mA, 60W mini power supply (Pg 86)
Ordering Information
horizontal unit with 15x7cm,
15x10cm & 15x15cm gel trays
& combs (Pg 13)
500V, 800mA, 300W maxi
power supply (Page 88)
MSSCREENTRIO-CS500KIT S creen Horizontal Electrophoresis Package with electrophoresis
starter kit, includes:
CSL-LOADDYE
MSMIDIDUO-NANO300
CSL-MDNA-1kb 1kb DNA ladder (Pg 139)
Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches
(Pg 138)
MSSCREENTRIO-CS500
MSMIDIDUO-NANO300KIT Mini Horizontal Electrophoresis Package with electrophoresis
starter kit, includes:
CSL-LOADDYE
100g agarose (Pg 136)
Ordering Information
MSMIDIDUO-NANO300 Midi Horizontal Electrophoresis Package, includes:
horizontal unit with 10x7cm
and 10x10cm gel trays and
combs (Pg 11)
CSL-TBEP
horizontal unit with 26x16cm,
MSSCREENTRIO 26x24cm & 26x32cm gel trays & CS-500V
combs (Pg 17)
Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches
(Pg 138)
Ordering Information
MSMIDIDUO
Choice Horizontal
with Power Supply
CSL-MDNA-1kb 1kb DNA ladder (Pg 139)
300V, 400mA, 60W mini power supply (Pg 86)
MSMINIDUO-NANO300KIT Mini Horizontal Electrophoresis Package with electrophoresis
starter kit, includes:
10x Bromophenol Blue Loading
CSL-AG100
Dye, 1ml (Pg 140)
multiSUB™-4
Horizontal with
Power Supply
CSL-TBEP
100g agarose (Pg 136)
Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches
(Pg 138)
Ordering Information
MSUB4-CS300 MultiSUB-4 Horizontal Electrophoresis, includes:
MULTISUB-4
horizontal unit with four
8x12cm gel trays and combs
(Page 22)
CS-300V
300V, 700mA, 150W midi
power supply (Page 87)
MSUB4-CS300KIT M
ultiSUB-4 Horizontal Electrophoresis Package with electrophoresis
starter kit, includes:
MSUB4-CS300
CSL-LOADDYE
10x Bromophenol Blue Loading
CSL-AG100
Dye, 1ml (Pg 140)
CSL-MDNA-1kb 1kb DNA ladder (Pg 139)
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
CSL-TBEP
100g agarose (Pg 136)
Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches
(Pg 138)
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
28
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 29
Real-time size fractionation and recovery of
nucleic acids
The Cleaver
Scientific SAFE
SERIES
features:
• Contains everything except chemicals and reagents
Cleaver Scientific’s ‘Safe Series’ has been developed to
address the needs of researchers looking to work with safer
alternatives to UV irradiation and ethidium bromide, both of
which are known to have harmful mutagenic effects. Since
starting with its award-winning* runVIEW™ electrophoresis
system, the ‘Safe Series’ now includes the runDOC imaging
system and its repertoire of runSAFE non-mutagenic, blue
light fluorescent stains.
*Best Product Award at Lab Innovations 2013
to perform horizontal electrophoresis and real-time
gel free DNA band extraction and purification from
runSAFE, and EtBr- and SYBR-stained agarose gels
• Combines the high resolution capability of the
MSCHOICE system with the time-and space-saving
convenience of a power supply and gel illuminator
integrated within a compact bench top unit
• One viewing lid supplied, containing an amber
emission filter for runSAFE and SYBR-stained gels
which includes an extractor fan to keep the viewing
pane free of condensation during electrophoresis
• Optional gel documentation system fits directly over
the base unit and gel tank for imaging at the end of the
electrophoresis run
• Perfect for education – let students learn the
TECHNICAL SPECIFICATION
cost of a separate gel tank, power supply and
transilluminator
Real time Electrophoresis. Perfect for running and tracking
dna without harmful UV.
To use runVIEW™ for gel-free DNA recovery is simple.
1. C
ast a gel with two identical rows of wells using one of the
matching pairs of 3mm combs supplied. Transfer to the
tank, remove the combs, and add buffer just to cover the
gel and fill the wells. Load DNA (‘loading tier’).
2. R
eplace the lid and apply the power. Turn on the blue LED
and see the DNA samples migrate in real-time.
Transilluminator
Wavelength
470nm
Timer
1-999 minutes with alarm
Voltage/ Resolution
25-150V / 1V
Safety Device
No load detection
Track DNA without harmful UV
Current/ Resolution
300mA / 1mA
Operating
Temperature
Ambient to 40°C
Power
30 W
Dimensions
293 x 220 x 80 mm
Operating
Mode
Constant Voltage
or Current
Rated
Voltage
100-240V, 50/60Hz
bluVIEW
Lid Design
CSL-RVLID1 - bluVIEW
lid - bluVIEW lid – Amber
(runSAFE & SYBR stains);
Built-in extractor fan
powered by base unit
runVIEW™ Gel System
Gel Dimensions
(W X L)
Unit dimensions
(W X D X H)
Buffer volume
Typical Applications
Simple DNA recovery
3. W
hen the DNA bands of interest enter the second row
of wells (‘extraction tier’), stop the power, remove lid and
harvest the DNA by pipette.
runVIEW™ Viewing Dock
15 X 7, 15 x 10 &
15 X 15cm
principles of electrophoresis by witnessing DNA
size fractionation as it happens in the gel, in total
safety from potentially hazardous UV and ethidium
bromide!
• Money-saving – 30-50% less than the combined
runVIEW™ is an innovative new system which can be used
to maximise the efficiency of DNA recovery from runSAFE,
and EtBr- and SYBR-stained gels by minimising the number of
steps involved in post-electrophoretic purification. runVIEW™
comprises the multiSUB™ MSCHOICE system with bluVIEW
lid, containing an amber emission filter within its viewing
pane, and a base unit with integrated power supply and
blue LED gel illuminator. The amber emission filter has
been optimised for safe, green-fluorescence emitting stains,
including SYBR Green and runSAFE, while an additional
bluVIEW lid, which is optional, contains an orange emission
filter for EtBr and other red-fluorescence emitting stains.
Both lids benefit from built-in extractor fans powered by
the base unit for condensation-free viewing of real-time
electrophoresis.
26.5 X 17.5 X
9cm
500ml
Combs
Comb
Thickness
2 x extra thick 3mm
preparatory combs double
sided, 4 & 16MC and also
20/28MC.
2 x 1mm doubled sided
combs, 4 & 16MC and 4
x double sided combs 20
& 28MC
1&3mm
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Blue light is safe and has none of the detrimental effects
of UV transillumination, including mutagenesis which can
compromise cloning efficiency, while runVIEW™’s capacity to
provide real-time visualisation of electrophoresis enables DNA
to be tracked as it migrates through the gel. This allows the
user to judge precisely when the band of interest is ready for
extraction.
and EtBr gels cast within the 15x7, 15x10 or 15x15cm
MSCHOICE gel trays, and therefore does not require expensive precast gels and accessories.
A self-contained system
The compact base unit, which houses the in-built power supply and blue LED transilluminator, is dual-voltage and portable, and allows electrophoresis, gel visualisation and extraction
to be performed at the bench, without the inconvenience of
having to transport gels to a darkroom elsewhere within the
laboratory.
Other benefits offered by runVIEW™ include:
• Power supply integrated within the base unit – adjustable
in 1V or 1mA increments; timer function to 999 minutes for
extended runs.
• Specialist combs for specialist applications - four multichannel
compatible 1mm 20-/28-sample combs for rapid screening of nucleic acids from 96-well thermal cycler blocks and
microplates. 3mm preparatory combs supplied for enhanced
DNA recovery.
• Get real-time - existing MSCHOICE users may customise
their system by purchasing the base station and bluVIEW lid.
• Versatile - power supply and gel illuminator may be used
with other gels and electrophoresis systems.
ORDERING INFORMATION
CSLRVMSCHOICE7
runVIEW™ system complete with 15 x 7cm gel tray, 1
set of casting dams & 8 combs.
CSLRVMSCHOICE10
runVIEW™ system complete with 15 x 10cm gel tray, 1
set of casting dams & 8 combs.
CSLRVMSCHOICE15
runVIEW™ system complete with 15 x 15cm gel tray, 1
set of casting dams & 8 combs.
CSLRVMSCHOICETRIO
runVIEW™ system complete with 15 x 7, 15 x 10 and
15 x 15cm gel trays, 3 sets of casting dams & 8 combs.
CSL-RVLID1
runVIEW™ bluVIEW lid MS15-1/2-1
Orange (EtBr) optional
Comb 1 & 2
sample, 1mm
thick
CSL-RVLID2
runVIEW™ bluVIEW
lid - Amber (runSAFE)
standard
MS15-4/10MC-1
Comb 4 &
10MC sample,
1mm thick
CSL-RVHOOD
runVIEW™ VIEWING
HOOD
MS15-20/28MC-1
Comb 20
&28MC sample,
1mm thick
CSL-RVBSBVLID
runVIEW™ base station
& bluVIEW lid (RVLid2)
MS15-4/2M-3
Comb 4 sample
2 Marker, 3mm
thick
CSL-RVBLED-MOD
Replacement blue
light module, includes:
MMPCB-BLUE-LEDMTX-V3-1 (blue light
LED) and PPL-20-SIL37-5-2M (silicone heat
dispersion pad)
MS15-6/2M-3
Comb 6 sample
2 Marker, 3mm
thick
Save on time-consuming gel elution techniques
Running DNA into a well containing buffer effectively purifies
the DNA of agarose, eliminating the need for time-consuming gel excision and purification techniques that also result
in sample loss. Once harvested, the DNA needs only to be
cleaned by a straightforward ethanol precipitation when it is
ready for further digestion or ligation.
No expensive commercial gels
runVIEW™ works with standard runSAFE, SYBR Green
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
30
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 31
runDOC
Gel documentation for
real-time horizontal
electrophoresis
runDOC is a portable, lightweight gel documentation system
designed exclusively for the runVIEW™ real-time horizontal
electrophoresis system (Pg 28). runDOC comprises a
darkroom hood and 12.1 megapixel digital camera to
capture images of runVIEW™, and EtBr- and SYBR-stained
gels. The runDOC darkroom is placed directly over the
RVMSCHOICE gel tank located on the runVIEW™ base unit,
which contains a blue LED gel illuminator. The blue LED
illuminator provides the excitation source for the DNA gel
located within the tank, and following excitation the light
emitted is then visualised using either one of the bluVIEW lid
options supplied with runVIEW™ or the runDOC filter slide.
runDOC has a small footprint area, occupying minimal space
within the laboratory.
features:
features:
• Gel documentation system designed exclusively for
• Safe – all four runSAFE stains have ultra-low toxicity
• Lightweight darkroom hood fits quickly and easily
• Convenient – each stain is supplied as a ready to use
runVIEW™
over gel tank and base unit, allowing gels to be
imaged as the bands migrate in real-time or at the
end of the electrophoresis run
• Extractor fans in the bluVIEW lid and runDOC
darkroom hood keep the gel free of condensation
when imaging both during and after electrophoresis
• High resolution 12.1 megapixel camera with 8GB
memory card for storage of images in RAW and
JPEG formats
• Interchangeable filter slide and bluVIEW filter
options for full flexibility: amber filter for runSAFE,
SYBR and green fluorescence; and orange filter for
EtBr and red fluorescence
• runDOC may be also used like an ordinary gel
documentation system by placing the gel tray
directly on the runVIEW™ base station, which acts
as a transilluminator
Type
6x Loading dye; simply add 1 part stain to 5 parts
DNA, mix and load your gel
• Fast – no time-consuming post-staining or destaining of gels is required.
• Sensitive – very low background staining of the gel;
detects as little as 0.2ng DNA per band
• Improved cloning efficiency – does not damage
or mutate DNA, and does not affect downstream
cloning applications such as ligation
• Flexible – each stain may be used with Blue or UV
light
• runVIEW™ compatible – all four stains are perfect
for use with the runVIEW™ real-time horizontal gel
electrophoresis system supplied with bluVIEW amber
filter lid (page )
• Complete system with laptop and software available
The runSAFE Range - Less DNA damage,
improved cloning efficiency
TECHNICAL SPECIFICATION
Camera
(LC>5000mg/kg) and lack cell permeability
Lens
5x optical / 4x digital
zoom
Focal Length
6.1-30.5mm
DIGIC-5 processor
Effective Pixels
12.1MP
Aperture
f/1.8(W)-f/2.9(T)
Image
Resolution
640x480 up to
4000x3000
Shutter
Speed
15-1/4000s
Sensor
1/1.7” type high
sensitivity CMOS
Filters
File Format
RAW, JPEG
Computer
Interface
USB 2.0 Hi-speed (miniB-jack)
Video Out
NTSC / PAL
runDOC
filter slide
bluVIEW lid
options*
Weight /
Dimensions
(WxDxH)
Power
Laptop
requirements
Four safe stains for
real-time horizontal
electrophoresis
The runSAFE Range comprises four non-mutagenic stains
that produce instant visualisation of DNA bands upon
irradiation with blue light or UV in agarose or polyacrylamide
gels. Each stain is conveniently supplied in a 6x loading
dye which is mixed with 5 parts double-stranded DNA
before loading onto an agarose or polyacrylamide gel. The
individual colour constituents within each loading dye track
electrophoresis run progression, and indicate the extent of
band separation and resolution by co-migrating with DNA
bands of known molecular size, which varies according
to the gel percentage. All four stains are non-toxic, safe
for the environment and can be disposed of in the regular
laboratory waste without using expensive decontamination
methods. Each stain is sensitive and binds DNA to detect as
little as 0.2ng DNA per band within a gel; while gel imaging
is best performed using the amber emission filter found of
the bluVIEW lid or runDOC filter slide. The runSAFE Range
is comprised as follows:
• runSAFE – general purpose stain for DNA ranging from
50bp markers to large super-coiled plasmid
• runSAFE-PLUS500 – for DNA larger than 500bp in size;
• runSAFE-2000 – for DNA vectors and inserts ranging from
500-2000bp;
Orange filter for EtBr;
Amber filter for SYBR and
runSAFE
• runSAFE-500 – for small DNA fragments, PCR products,
sequence tracts and primers less than 500bp.
bluVIEW-orange for EtBr
bluVIEW-amber for SYBR
and runSAFE
ORDERING INFORMATION
General
Storage Media
The runSAFE
Range
Part Number
Description
Tracking Dyes
Size Range
CSL-RUNSAFE
runSAFE stain, 1ml
Bromophenol Blue,
Xylene Cyanol FF,
Orange G
50bp – 20Kb
CSL-RUNSAFEPLUS500
CSL-RUNSAFE2000
CSL-RUNSAFE500
runSAFE-PLUS500
stain, 1ml
8GB SD Memory Card
ORDERING INFORMATION
0.8Kg / cm
Rechargeable Li-ion battery and plug-in charger
Code
Optional mains cable charger available
CSL-RVGELDOC
1.8GHz Pentium® IV or equivalent AMD Athlon® processor; 512MB
memory; operating system Windows® XP SP3 onwards; 1GB storage
and CD-ROM drive; 1 USB port 2.0; video resolution: 1280 x 800
CSL-RVGELDOCSYS
Camera specification may change.
RVGELDOC-F1
RVGELDOC-F2
CSL-CAMCHARGER
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
CSL-RVSTATION
CSL-RVGDCOMPLETE
Description
runVIEW™ gel Documentation hood with
12.1MP camera
runVIEW™ gel Documentation hood with
camera, Laptop & 1D Analysis Software
Orange Filter for runDOC (Ethidium
Bromide)
Amber Filter for runDOC (runSAFE and SYBR
stains)
Optional mains charger cable for 12.1MP
camera
runSTATION complete with CSL-RVGELDOC
& CSL-RVMSCHOICETRIO (Pg 28)
RVGDCOMPLETE includes RVGELDOCSYS
and RVMSCHOICETRIO
Slower migrating species, indicative of a linear or relaxed
circular vector, resulting from DNA nicking or strand breaks,
are significantly reduced in DNA plasmid mixed with runSAFE and exposed to blue light. The concentration of nicked
DNA plasmid increases significantly after 8’ of exposure to
EtBR and UV irradiation.
runSAFE- 2000, 1ml
runSAFE-500, 1ml
Bromophenol Blue,
Xylene Cyanol Blue
Xylene Cyanol Blue,
Orange G
Orange G
>500bp
500-2000bp
<500bp
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
32
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 33
ORDERING INFORMATION
runVIEW™ CONVERTER package (to convert standard MSMINI &
MSMIDI units to real-time electrophoresis)
CSL-RVBSBVLID-MINI
runVIEW™ base station & bluVIEW lid for MSMINI systems
CSL-RVBSBVLID-MIDI
runVIEW™ base station & bluVIEW lid for MSMIDI systems
runVIEW™ STANDARD package (for those users with their own power
supply)
TECHNICAL SPECIFICATIONS
runVIEW™ Adjustable Viewing Dock for runVIEW™ MINI & MIDI
features:
Blue Light
Wavelength
470nm
Dimensions
(WxDxH)
•Complete versatility - 4 different
Operating Temperature
Ambient to
40°C
Rated Voltage
100-240V,
50/60Hz
runVIEW™ MINI Gel System
Gel Dimensions
(WxL)
7x7, 7x10cm
bluVIEW lid
design
Amber spectral
emission filter with
built-in extractor
fan powered by
the base unit
Unit Dimensions
(WxDxH)
9x2, 1x9cm
Included
Combs*
2x 8-sample
runVIEW™ COMPLETE package (complete real-time electrophoresis
system with power supply)
Buffer Volume
225ml
Comb
Thickness
1mm
CSL-RVMSMINI-C
CSL-RVMSMINI-S plus NANOPAC-300 (Pg 86)
runVIEW™ MIDI Gel System
CSL-RVMSMIDI-C
CSL-RVMSMIDI-S plus NANOPAC-300 (Pg 86)
Gel Dimensions
(WxL)
bluVIEW lid
design
Amber spectral
emission filter with
built-in extractor
fan powered by
the base unit
CSL-RVMSMINI-S
CSL-RVBSBVLID-MINI plus MSMINIDUO tank
with 7x7 & 7x10cm trays, 1 set of casting dams
and 2x 8-sample combs
CSL-RVMSMIDI-S
CSL-RVBSBVLID-MIDI plus MSMIDIDUO tank
with 10x7 & 10x10cm trays, 1 set of casting
dams and 2x 16-sample combs
runVIEW™ COMPLETE KIT package (complete real-time electrophoresis system with power supply & chemicals)
CSL-RVMSMINI-C plus reagent kit for real-time
electrophoresis (includes: CSL-RUNSAFE [1ml
runSAFE stain, Pg 31], CSL-MDNA [1kb DNA
CSL-RVMSMINI-CK
ladder, Pg 140], CSL-AG100 [100g agarose, Pg
136], & CSL-TBEP [Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches, Pg 138])
CSL-RVMSMIDI-C plus reagent kit for real-time
electrophoresis (includes: CSL-RUNSAFE [1ml
runSAFE stain, Pg 31], CSL-MDNA [1kb DNA
CSL-RVMSMIDI-CK
ladder, Pg 140], CSL-AG100 [100g agarose, Pg
136], & CSL-TBEP [Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches, Pg 138])
Accessories & Replacement Parts
CSL-RVRTKIT
runVIEW™ real-time reagent kit (includes:
CSL-RUNSAFE [1ml runSAFE stain, Pg 31],
CSL-MDNA [1kb DNA ladder, Pg 140], CSLAG100 [100g agarose, Pg 136], & CSL-TBEP
[Powdered Tris-Borate-EDTA Running Buffer –
10 pouches, Pg 138])
NANOPAC-300
nanoPAC-300 power supply (300V, 400mA,
60W), Pg 86
MS7-UV7
7x7cm UV tray MS7-UV10
7x10cm UV
tray
MS7-UVDAM
MS7 casting
dams, 1x set
MS7-8-1
Comb 8 sample, 1mm thick
MS10-UV7
10x7cm UV
tray
MS10-UV10
10x10cm UV
tray
MS10-UVDAM
MS10 casting
dams, 1x set
MS10-16-1
Comb 16
sample, 1mm
thick
CSL-RVLID2MINI
runVIEW™
bluVIEW
lid - Amber
(runSAFE) MSMINI
CSL-RVLID2MIDI
unVIEW bluVIEW lid - Amber (runSAFE)
- MSMIDI
10x7,
10x10cm
Unit Dimensions
(WxDxH)
12.5x22x9cm
Included
Combs*
2x 16-sample
Buffer Volume
300ml
Comb Thickness
1mm
10-300V / 1V
Power
Current / Resolution
400mA / 1mA Timer
Operating Mode
Constant
Voltage or
Current
Rated Voltage
• runVIEW™ CONVERTER
package - with emission
filter lid and blue light
illuminator, to allow standard
MSMINI and MSMINI units
to be converted to real-time
electrophoresis;
• runVIEW™ STANDARD
package – includes blue light
illuminator, and runVIEW™
MINI or MIDI tank, for those
users with their own power
supply;
• runVIEW™ COMPLETE –
runVIEW™ BASIC package
in MINI or MIDI format with
nanoPAC-300 power supply;
• runVIEW™ COMPLETE KIT
nanoPAC-300 Power Supply option (supplied with runVIEW™-COMPLETE systems only)
Voltage / Resolution
types of runVIEW™ package
are available in MINI and MIDI
formats:
60W maximum
1-999 minutes
with alarm /
Continuous
100-240V,
50/60Hz
*For other comb options for the runVIEW™ MINI & MIDI please see
pages 8 and 10.
– runVIEW™ COMPLETE
MINI or MIDI package with
electrophoresis reagent kit.
•Portable adjustable blue light
illuminator for use with MSMINI
and MSMIDI electrophoresis
tanks
•Blue light is completely safe to
both operator and DNA alike,
and results in improved cloning
efficiency compared to UV
•Emission filter lid with built-
in extractor fan enables
condensation-free viewing
of blue-light fluorescent gels,
containing SYBR Green, SYBR
Safe and runSAFE stains
•runDOC-MINI enables
visualisation of real-time
electrophoresis of MSMINI and
MSMIDI gels using adjustable
blue light illuminator
•Electrophoresis kit available
containing agarose, 10x Trisborate electrophoresis buffer,
1kb DNA markers and runSAFE
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Quick checks of low to medium
numbers of samples following PCR and
cloning. Education
RunVIEW™ MIDI
& MINI Systems
Continuing from the success of the
award-winning runVIEW™ system,
the runVIEW™ MINI and MIDI are
Cleaver Scientific’s latest additions
to its Safe Range of electrophoresis
products. Both systems benefit
from the blue light illumination of
fluorescently stained 7- or 10-cm-wide
agarose gels to allow users to view
the size-fractionation of nucleic acids
in real-time. An adjustable blue-light
illuminator platform accommodates
both the MSMINI and MSMIDI
electrophoresis tanks, while band
visualisation is achieved through the
corresponding lid containing an amber
spectral emission filter. Each lid remains
free of condensation by a built-in
extractor fan and may be connected
either to the user’s own power supply
or to the optional, but recommended,
nanoPAC-300.
34
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 35
COMBS
ORDERING INFORMATION
TGT6XMINI
Teaching gel tank to run 6x 7x7cm gel trays, includes:
12x 8-sample, multichannel compatible combs, 12x
casting dams, loading guides and cables
features:
Sample
volume
for a 5mm
thick gel
Code
Description
MS7-1-1
Comb Prep 1, Marker 1, 1mm thick
203µl
TGT6XMININANO300
TGT6XMINI supplied with nanoPAC-300 power supply
MS7-2-1
Comb Prep 2, Marker 2, 1mm thick
90µl
MS7-4-1
Comb Prep 4, Marker 2, 1mm thick
48µl
TGT6XMININANO300KIT
TGT6XMINI supplied with nanoPAC-300 power supply
and electrophoresis kit, including 1x CSL-RUNSAFE
(1ml, runSAFE stain mixed with loading dye), 1x CSLMDNA-1KB (1ml, 1kb DNA ladder), 1x CSL-AG100
(100g Agarose), CSL-TBEP (Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches)
MS7-8MC-1
Comb 8 sample MC, 1mm thick
11µl
MS7-8-1
Comb 8 sample, 1mm thick
25µl
MS7-10-1
Comb 10 sample, 1mm thick
18µl
TGT4XMINI
Teaching gel tank to run 4x 7x7cm gel trays, includes:
8x 8-sample, multichannel compatible combs, 8x casting
dams, loading guides and cables
MS7-12MC-1
Comb 12 sample MC, 1mm thick
14µl
MS7-16-1
Comb 16 sample, 1mm thick
10µl
TGT4XMININANO300
MS7-1-1.5
Comb Prep 1, Marker 1, 1.5mm thick
304µl
TGT4XMINI supplied with nanoPAC-300 power supply
MS7-2-1.5
Comb Prep 2, Marker 2, 1.5mm thick
135µl
MS7-4-1.5
Comb Prep 4, Marker 2, 1.5mm thick
72µl
TGT4XMININANO300KIT
TGT4XMINI supplied with nanoPAC-300 power supply
and electrophoresis kit, including 1x CSL-RUNSAFE
(1ml, runSAFE stain mixed with loading dye), 1x CSLMDNA-1KB (1ml, 1kb DNA ladder), 1x CSL-AG100
(100g Agarose), CSL-TBEP (Powdered Tris-Borate-EDTA
Running Buffer – 10 pouches)
MS7-8MC-1.5
Comb 8 sample MC, 1.5mm thick
17µl
MS7-8-1.5
Comb 8 sample, 1.5mm thick
37µl
MS7-10-1.5
Comb 10 sample, 1.5mm thick
27µl
TGT6XMINI4CS300
TGT6XMINI classroom workstation for 24 students,
includes: 4x TGT6XMINI units and 1x CS-300V power
supply
MS7-12MC-1.5
Comb 12 sample MC, 1.5mm thick
20µl
MS7-16-1.5
Comb 16 sample, 1.5mm thick
15µl
MS7-1-2
Comb Prep 1, Marker 1, 2mm thick
405µl
TGT6XMINI4CS300KIT
TGT6XMINI classroom workstation for 24 students,
includes: 4x TGT6XMINI units and 1x CS-300V power
supply and 4x electrophoresis kits with runSAFE, 1kb DNA
ladder, agarose and running buffer
MS7-2-2
Comb Prep 2, Marker 2, 2mm thick
180µl
MS7-4-2
Comb Prep 4, Marker 2, 2mm thick
96µl
TGT-UV7
7x7cm gel tray
MS7-8MC-2
Comb 8 sample MC, 2mm thick
23µl
MS7-8-2
Comb 8 sample, 2mm thick
50µl
MS7-10-2
Comb 10 sample, 2mm thick
36µl
MS7-12MC-2
Comb 12 sample MC, 2mm thick
27µl
MS7-16-2
Comb 16 sample, 2mm thick
20µl
MS7-UVDAM
MS15-PE
MS15-NE
MS20-PE
MS20-NE
MS15/20-FC
MS7-LG
Casting dams,
pk/2
Positive
electrode,
TGT4XMINI
Negative
electrode,
TGT4XMINI
Positive
electrode,
TGT6XMINI
Negative
electrode,
TGT6XMINI
Viewing Platform,
TGT6XMINI
Buffer Saver Blocks,
MSCHOICEBSB pk/2, save 190ml
buffer
Buffer Saver Blocks,
MSMAXIBSB
pk/2, save 450ml
buffer
MS20-WP
MS7-UVS
7cm UV Gel Scoop
CSL-CAB
Electrophoresis cable
(black and red)
MS15-WP
Viewing Platform,
TGT4XMINI
nanoPAC-300 Mini
multiSUB
Power Supply, 300V,
Choice/Maxi
NANOPAC-300
400mA, 60W – 100Flexicaster
240VAC (Pg 86)
CS-300V Midi Power
Adhesive
Supply, 300V, 700mA,
CS-300V
Loading Guides
150W – 100-240VAC
(Pg 87)
Technical Specifications
TGT4XMINI
TGT6XMINI
Unit Dimensions
(WxLxH)
17.5x26.5x9cm
23x39.5x9cm
Gel Dimensions
(WxL)
7x7cm
7x7cm
Maximum Gel
Throughput
4x 7x7cm mini gels
6x 7x7cm mini gels
Standard Combs
8x 8-sample, 1-mmthick multichannel
compatible
(2 combs per gel)
12x 8-sample, 1-mm-thick
multichannel compatible (2
combs per gel)
Maximum Sample
Capacity
64 samples with
standard comb option;
128 samples using
optional 16-sample
combs
96 samples with standard
comb option; 192 samples
using optional 16-sample
combs
Buffer Volume
500ml
1200ml
Combs available:
1, 2, 4, 8MC, 8, 10,
12MC, 16
1, 2, 4, 8MC, 8, 10, 12MC,
16
• Two new Teaching Gel Tanks
for demonstration of horizontal
gel electrophoresis to groups of
students: the TGT6XMINI with six
7x7cm gel trays and TGT4XMINI
with four 7x7cm gel trays
• A teacher may demonstrate to
up to 6 students at a time using
only one TGT6XMINI with power
supply option, and 4 students
with the TGT4XMINI with power
supply option
• Two combs supplied per gel tray
provide maximum throughput of
96 samples for the TGT6XMINI,
and 64 samples with TGT4XMINI
• One set of leak-free plug-and-go
casting dams per gel tray
• UV-transparent gel trays provide
safe transport of fragile gels from
the tank to the transilluminator
for nucleic acid band visualisation,
minimising risk of exposure to
harmful ethidium bromide
• Mutagenic ethidium bromide
may be substituted by non-toxic
runSAFE supplied with chemical
kits
• Compact gel tank design with
small buffer volumes enables a
maximum of 96 samples to be run
comfortably within the time of a
typical university practical class
• Units may be supplied with
Number of samples 0.75, 1, 1.5, 2mm
electrophoresis chemicals and
nanoPAC-300 power supply (p.
X) for maximum convenience and
cost-effectiveness
• Complete class room kit available
with CS-300V power supply (p.
X) and four TGT6XMINI units to
demonstrate electrophoresis to up
to 24 students at a time
Teaching Gel Tanks
The Teaching Gel Tank Range is
Cleaver Scientific’s latest contribution
to electrophoresis in education. By
manufacturing two compact units
capable of running up to four and
six 7x7cm mini gels respectively, a
teacher may demonstrate horizontal
gel electrophoresis to as many students
using just a single gel tank and power
supply, to save on bench space, time
and cost. The Teaching Gel Tank
Range also benefits from all of the
high quality features of MultiSUB™ gel
0.75, 1, 1.5, 2mm
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Education – demonstrate the
technique of horizontal nucleic acid gel
electrophoresis to student classes
High throughput electrophoresis of
mini gels
tanks, including: leak-free plug-and-go
casting dams; UV-transparent gel trays;
height-adjustable combs; and easy-toreplace yet hard-to-break colour-coded
cassette electrodes.
Each gel tank is manufactured in a
robust injection moulded design, and
may be supplied with an optional
power supply, either with or without
DNA electrophoresis chemicals and
markers.
36
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 37
Technical Specification
Slide Capacity
Unit Dimensions
(w x l x h)
Volume
CSL-COM10
10
CSL-COM20
20
CSL-COM40
40
17x34x9cm
31x34x9cm
33x59x9cm
550ml
1000ml
2100ml
Recommended
Running
Conditions
25V/300mA for 1h
ORDERING INFORMATION
CSL-COM10
Comet Assay Tank for 10 Slides
CSL-COM20
Comet Assay Tank for 20 Slides
CSL-COM40
Comet Assay Tank for 40 Slides
CSL-CHILLER
Chiller unit for active slide temperature control
Full Screen Shot of a Comet Profile
A. The Comet ‘Head’ comprising largely intact genomic DNA.
B. The Comet ‘Tail’ of mobile damaged or fragmented DNA.
The Comet Assay
Background: First introduced in 1981 to quantify double-stranded DNA breakages in single cells exposed to
-irradiation, the Comet Assay (or SCGE) has since been
adapted to analyse specific DNA lesions and repair processes
to analyse the potential for DNA damage of new drugs and
environmental conditions.
Overview: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of supercoiled duplex
DNA reduces the size of the large genomic DNA from which
these strands are separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a
‘comet’ as its negatively charged broken ends and fragments
migrate towards the anode during electrophoresis.
Method: After exposure to a genotoxic insult cells are
suspended within low melting point agarose and embedded
within a thin layer of agarose on a microscope slide. Cellular
protein is then removed by lysis in detergent, when DNA is
allowed to unwind in alkaline conditions before electrophoresis. The DNA is electrophoresed, stained and then analysed
using software.
Results: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged cells the resultant
image resembles a ‘comet’ with the cellular DNA separated
into a head and tail. The head is mainly composed of intact
genomic DNA, whereas any fragmented or damaged DNA is
concentrated within and towards the tail.
features:
CSL Chiller
The CSL-CHILLER (see page 81 for full details) is available
ready assembled with the thermostat mounted on the refrigerator and supplied with insulated tubing and clips to form
a system ready to use. Supported by an industry leading 3
year warranty emphasising our confidence in the reliability
of our product.
The system comprises of the following:
• For Single Cell Gel
Electrophoresis
• Minimise exposure
to light and reduce
background DNA
damage
• High efficiency
cooling for
enhanced resolution
• 5 litre tank with drain tap
• relay control for refrigeration on/off
• cooling power 140W @ 0°C
• temperature range -20 to 100°C
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Quantifying dna damage and repair
in drug development application.
Reproductive science.
Comet Assay Tanks
Cleaver Scientific COMET assay tanks
are available in four slide formats to
study Single Cell Gel Electrophoresis
(SCGE), a technique made popular by
drug toxicology and carcinogenesis
studies for the detection and quantitation of DNA damage in cells.
Each tank’s robust construction from
ebony acrylic ensures that cells remain
free of exposure to background light
and DNA damage during electrophoresis, while a cooled central platform
provides a convenient surface for slide
preparation and control of slide temperature during the assay.
Following electrophoresis DNA damage
may be measured using Comet Assay
scoring software.
38
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 39
ORDERING INFORMATION
COMPAC-50
High Throughput Comet system for 50 slides, includes
2x 25-slidecarriers, 4x staining dishes, tank with ceramic
cooling platform and cool pack, lid and power cables
COMPAC50CS300
COMPAC-50 and CS-300V Power Supply 300V,
700mA, 150W
COMRAC-25
Vertical slide carrier for 25 slides, pk/1
STAINDISH
Ebony acrylic stain dish, pk/1
STAINDISH4X
Ebony acrylic stain dish, pk/4
COMPAC50-PE
Positive electrode
COMPAC50-NE
Negative electrode
CSL-CAB
Electrophoresis cables (black and red)
Related products
CS-300V
CS-300V Power Supply 30V, 700mA, 150 W (Pg 87)
CV2
Cleaver Pipette - Volume; 0.2 - 2µl (Pg 112)
CV10
Cleaver Pipette - Volume; 0.5 - 10µl (Pg 112)
CV20
Cleaver Pipette - Volume; 2 - 20µl (Pg 112)
CV100
Cleaver Pipette - Volume; 10 - 100µl (Pg 112)
CS-NRK
Rocking Shaker with 30 x 30cm platform and flat non
slip rubber mat (Pg 132)
CSL-NHYBRIDBASIC
Incubator only with 2 stainless steel shelves - 110/230
(Pg 127)
CSL-LMA50
Agarose 50g, Low melting point (Pg 136)
features:
• Unique patent pending design
employs two carriers, each
capable of accommodating
25 slides in a vertical laminar
orientation
• Slide carriers facilitate batch-
Typical Result
Repair of UVB-induced DNA damage in human keratinocytes, using enzyme-modified comet assay. HaCaT cells
were irradiated with 1 J/cm2 UVB, then allowed to repair in
fresh medium and DNA damage analysed at different time
points (A) 0 h, (B) 1 h,(C) 6 h, (D) unirradiated (courtesy of
Karbaschi, M. University of Leicester, Leicester, UK).
Unit Dimensions (WxLxH)
26.5 x 15 x 15cm
Total Slide Capacity
50 slides 25 x 75mm
Slide Capacity per Rack
25
Volume
550 ml
Recommended Running
Conditions
27V/450mA for 20 minutes
Recommended Power
Supply
CS-300V 300V, 700mA, 150W MIDI
batch-treatment of slides during
the lysis, neutralisation, staining
and washing steps
and staining dishes ensures that
nuclei remain free of exposure
to background light and potential
DNA damage
• Highly compact design optimises
COMPAC-50 – Experimental Procedure
COMPAC-50
• Ten staining dishes supplied for
• Ebony acrylic construction of tank
COMPAC-50 vertical slide carrier & Staining dish
Technical Specification
processing of multiple slides
simultaneously, thus eliminating
the need for manual handling of
individual slides, during pre- and
post-electrophoresis steps of
the Comet Assay, to maximise
processing speed
Overview of a typical comet assay procedure. A single-cell
suspension of the cells under investigation is mixed with low
melting point agarose. The cell/agarose mix is layered on
glass microscope slides, pre-coated with agarose, and the
agarose allowed to set. The cells are lysed under high pH
before washing with pure water. The presence of strand
breaks and high pH allows the cellular DNA to unwind.
Electrophoresis draws the DNA out of the nucleoid body
forming a ‘tail’. The amount of migration (the amount
of DNA in the tail versus the head) is proportional to the
initial amount of DNA damage. The slides are then drained,
neutralised and washed with pure water before drying overnight. Following further washing in pure water the slides are
stained, washed and finally scored and analysed, typically
using fluorescent microscopy and image analysis software.
Advantageously, the use of a slide-holder assembly, which
maintains multiple slides in laminar arrangement, also
permits batch processing of these slides through pre-electrophoresis and post electrophoresis steps, thus eliminating the
need for individual manipulation of samples
electrophoresis efficiency during
Comet Assay, compared to other
manufacturers’ systems
• Enhanced cooling is provided
by a ceramic cooling platform
with spring loaded platform that
accommodates low-cost prefrozen cool packs to maintain
optimal buffer temperature
• 50 slides may be run within 20
minutes using CS-300V power
supply (p. 87)
• Maximum 50 slide throughput
with higher throughput systems
to be launched soon
• Supported by a comprehensive
publication record, and the
technical expertise of our
academic partner: the Oxidative
Stress Group in the Department
of Cancer Studies and Molecular
Studies within the University of
Leicester
COMPAC-50™ High
Throughput Comet
Electrophoresis System
Developed in collaboration with
the Oxidative Stress Group in the
Department of Cancer Studies
and Molecular Medicine within
the University of Leicester, the
COMPAC-50 is a high throughput
electrophoresis system, available
exclusively through Cleaver Scientific,
to perform the Comet Assay,
otherwise known as Single Cell Gel
Electrophoresis. A unique patent
pending design employs two carriers
to hold a total of 50 slides (25 per
carrier) vertically. This provides two
distinct advantages over conventional
Comet Assay systems that utilise
a horizontal platform for manual
mounting of multiple individual slides.
Firstly to produce a highly compact
system which saves 75% of Lab space.
Secondly by holding 25 slides in a
rack this allows slides to be processed
together in one batch saving on
handling assay time by up to 90%.
Consequently, this is not only beneficial
for electrophoresis but also in the lysis,
neutralisation, staining and washing
steps of the Comet Assay, when each
batch of slides may be treated during
each step respectively using the four
ebony acrylic staining dishes supplied.
In addition, the COMPAC-50 benefits
from a high performance ceramic
cooling base with sliding drawer to
accommodate a cool pack, which is
frozen before use, to maintain optimal
buffer temperature.
The COMPAC-50 marks the first of
a new range of innovative systems
designed specifically for rapid
high throughput single cell gel
electrophoresis.
A product developed
in partnership with
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
DNA repair, mutagenesis and oxidative stress research. Drug toxicology studies.
Food safety testing
40
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 41
CSL-CELLAS
Horizontal Unit for Cellulose Acetate Electrophoresis
Cleaver Scientific also provides a comprehensive range of
‘wet’ cellulose acetate gels, consumables and diagnostic kits.
These include:
nanoPAC-300
300V Power supply, 400mA, 60W (Pg 86)
CellasGEL – ‘wet’ cellulose acetate gels
Code
Size (w x l) Description
CSLGEL2.514250
2.5x14cm
CellasGEL 250μm, standard thickness, 100/pack
CSLGEL2.514200
2.5x14cm
CellasGEL 200μm, standard thickness, 100/pack
CSLGEL2.514190
2.5x14cm
CellasGEL 190μm, high resolution, 100/pack
CSLGEL5.714500
5.7x14cm
CellasGEL 500μm, high volume, 25/pack
CSLGEL5.714250
5.7x14cm
CellasGEL 250μm, standard thickness, 25/pack
CSLGEL5.714200
5.7x14cm
CellasGEL 200μm,standard thickness, 25/pack
CSLGEL5.714190
5.7x14cm
CellasGEL 190μm, high resolution, 25/pack
CSLGEL2.517200
2.5x17cm
CellasGEL 200 micron, 25/pack
ORDERING INFORMATION
CellasGEL ‘wet’ cellulose acetate gel strips are ready to
use and overcome many of limitations of traditional ‘dry’
cellulose acetate membranes. CellasGEL’s advantages over
dry cellulose acetate membranes are as follows:
1. Wet state – unlike dry membranes, CellasGEL is a
cellulose acetate film produced in a wet form to facilitate
buffer adsorption, but without the entrapment of air
bubbles that inhibit electrophoresis
2. Greater thickness – CellasGEL’s greater thickness (190500μm) compared to dry membranes (160-190μm)
allows application of larger sample volumes to enhance
detection of poor quality specimens low in protein
content
3. High resolution – samples may be applied to CellasGEL
as wider but finer bands, without risk of diffusion, to
make band quantitation more reproducible; this is further
enhanced by extended migration distances (60-70mm)
that improve band separation
Using CellasGEL
1. Equilibrate a CellasGEL for 10’ in electrophoresis buffer using an
agitating platform (e.g. 3D Shaker, Pg 119).
4. Amphiphilic – CellasGEL’s lipophilic and hydrophilic
properties make it the perfect separation medium for
many different biological molecules, ranging from
lipoproteins to haemoglobins
CellasGEL is supplied either as individual packs of 25 or 100
strips or within clinical test kits for the following applications:
2. Dry surplus buffer from the CellasGEL before securing it to a
Bridge located within a pre-prepared Cellas tank.
3. A
pply samples to the CellasGEL using the appropriate Applicator,
and electrophorese at 200V for 30-90’ (see Power Supplies, Pg 86).
4. Remove the CellasGEL from the tank, and use the required Clinical
Test Kit for staining and destaining and clearing.
• Immunofixation Electrophoresis (IFE): Monoclonal
Gammopathies of Undetermined Significance (MGUS);
Multiple Myeloma (MM)
• Serum Protein Analysis: Dysproteinaemia; Incipient
Gammopathies
• Haemoglobin Analysis: Haemoglobinopathies such as
Thalassaemias and Sickle Cell Disorders
• Lipoprotein Analysis: Hyperlipidaemias; High-density
(HDL), Low-density (LDL) and Very-low-density (VLDL)
lipoprotein evaluation
features:
• Compact high
resolution system
for clinical
electrophoresis
• Accommodates
strips and gels up to
24x20cm
• Complete range of
cellulose acetate
gels and kits
• Densitometer
software and
scanner available
Clinical Electrophoresis
Cellulose acetate electrophoresis is
an important technique in clinical
diagnostics. Cleaver Scientific’s
CellasGEL range is a complete solution
for research and clinical cellulose
acetate electrophoresis applications.
The CellasGEL range includes both
equipment and consumables to assist
in the research and diagnosis of specific
disease states.
Cellas Electrophoresis System
The ideal tank for standard ‘dry’
membrane and ‘wet’ gel cellulose
acetate techniques, the Cellas
electrophoresis system is designed
and built to our high quality standard
to address both routine clinical and
research requirements. Two adjustable
supports, which can be positioned
anywhere within the tank, readily
accommodate different lengths of
dry cellulose acetate membrane to a
maximum 20cm.
CSLAPPS4
Various Bridges
5. P
lace the CellasGELon a suitably sized mylar sheet or glass plate and
dry in an oven for 10’ at 80°C (e.g. CSL-NHYBRIDBASIC, Pg 127)
6. Quantify bands using Scanner and Densitometer Software.
Other gels, membranes, strips, bridges & applicators
available – PLEASE ENQUIRE
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Qualitative identification and
quantification of Hb variants. Finding
abnormalities of Hb synthesis like sickle
cell disorders, thalassaemias etc.
42
I HORIZONTAL GEL SYSTEMS
HORIZONTAL GEL SYSTEMS I 43
CellasGEL Wet Membranes
Ordering Information
CellasGEL Applications Packages equipped with bridges, applicator and clinical test kit
Clinical Test Kit
Code
Description
CellasKIT: 1-patient test for serum and concentrated urine IFE
2x semi-micro tests (1x serum and 1x urine) for 1 patient
performed on 6x CellasGEL strips (2.5x14cm; 190μm) on 3x
bridges in 1x CSL-CELLAS*.
CSLKITCU
Kit content, sufficient for 5 patients (10x semi-micro tests):
30 CellasGEL strips & TGS buffer; Coomassie stain, clearing &
saline solutions; volumetric distributors & antisera (anti- IgG, IgA,
IgM, Ig & Ig); blotting paper & mylar film. Excludes: Destain.
CellasKIT: 6-patient test for serum IFE
6x semi-micro or 8x micro tests (1/patient) on 6x CellasGEL
strips (5.7x14 cm; 190μm), performed on 6x bridges in 2x CSLCELLAS units**.
CSLKITI2432
CSLKITSP100200
Accessories Required
Applicator
1x
CSLAPPS22
(semi-micro)
3x
CSLBDG8.5S
CSLKITCU-ABS
Includes:
1x CSLKITCU
1x CSLAPPS22,
3x CSLBDG8.5S
1x CSLAPPS6 6x
(semi-micro) CSLBDG8.5S
1x
CSLAPPS4SP
(semi-micro)
CSLKITI2432-ABS
Includes:
1x CSLKITI2432,
1x CSLAPPS6,
6x CSLBDG8.5S
6x
CSLBDG8.5S
CSLKITI2432-ABM
Includes:
1x CSLKITI2432,
1x CSLAPP8M
6x CSLBDG8.5S
3x
CSLBDG8.5S
CSLKITSP-ABS
Includes:
1x CSLKITSP100200
1x CSLAPPS4,
3x CSLBDG8.5S
Kit content, sufficient for 100x semi-micro or 200x micro tests:
25 CellasGEL strips & Tris-Hippurate buffer; Ponceau S stain,
destain & clearing solutions; blotting paper & mylar film.
1x
CSLAPPM8
(micro)
3x
CSLBDG8.5S
CSLKITSP-ABM
Includes:
1x CSLKITSP100200
1x CSLAPPM8,
3x CSLBDG8.5S
CellasKIT: serum proteins (high resolution)
6x semi-micro or 8x micro tests for each CellasGEL strip
(5.7x14cm; 190μm); 3 strips / CSL-CELLAS* performed on 3x
bridges.
1x CSLAPPS6 3x
(semi-micro) CSLBDG8.5S
CSLKITSPHR-ABS
Includes:
1x CSLKITSP150200
1x CSLAPPS4,
3x CSLBDG8.5S
1x
CSLAPPM8
(micro)
CSLKITSPHR-ABM
Includes:
1x CSLKITSP150200
1x CSLAPPM8,
3x CSLBDG8.5S
CSLKITSP150200
Kit content, sufficient for 150x semi-micro or 200x micro tests:
25 CellasGEL strips & TGS buffer; Coomassie stain, citric acid &
clearing solutions; blotting paper & mylar film.
Excludes: Destain.
CellasKIT: haemoglobin
4x semi-micro tests for each CellasGEL strip (5.7x14cm; 250μm);
3 strips / CSL-CELLAS* performed on 3x bridges.
CSLKITHG100
Kit content, sufficient for 100x semi-micro tests:
25 CellasGEL strips & Tris-Glycine buffer; Ponceau S stain,
destain & clearing solutions; blotting paper & mylar film.
CellasKIT: lipoproteins
4x semi-micro tests for each CellasGEL strip (5.7x14cm; 250μm);
3 strips / CSL-CELLAS* performed on 3x bridges.
CSLKITLP100
Kit content, sufficient for 100x semi-micro tests:
25 Cellogel strips & Tris- Hippurate buffer; Sudan Black stain &
clearing solution; blotting paper & mylar film.
MGUS,
MM
CSLAPPS22
Kit content, sufficient for 24 patients (semi-micro) & 32 (micro):
24 CellasGEL strips & Tris- Hippurate buffer; Amidoblack stain,
1x
saline & clearing solutions; volumetric distributors & antisera
CSLAPPM8
(anti- IgG, IgA, IgM, Ig & Ig); blotting paper & mylar film.
(micro)
Excludes: Destain.
CellasKIT: serum proteins
4x semi-micro or 8x micro tests for each CellasGEL strip
(5.7x14cm; 200μm); 3 strips / CSL-CELLAS* performed on 3x
bridges.
Bridges
Applicators
Package (Kit + Accessories) Diagnostic
Application
Code
3x
CSLBDG8.5S
1x CSLAPPS4 3x
(semi-micro) CSLBDG8.5S
1x CSLAPPS4 3x
(semi-micro) CSLBDG8.5S
CSLKITHG100-ABS
Includes:
1x CSLKITHG100,
1x CSLAPPS4
3x CSLBDG8.5S
CSLKITLP100-ABS
Includes:
1x CSLKITLP100
1x CSLAPPS4
3x CSLBDG8.5S
CSLAPPS4SP
Unlike dry cellulose acetate strips that are restricted to low
volume micro tests, CellasGEL’s greater thickness allows
tests to be performed on semi-micro and macro scales using
a wide range of specialist applicators. Consequently greater
sample-volumes may be loaded as larger, but finer bands
over a wider front. This reduces sample saturation and
aids densitometric band quantitation, thereby improving
resolution.
Bridges
MGUS, MM
A universal bridge (CSLBDG8.5S) supports each 2.5x14cm
and 5.7x14cm CellasGEL during sample application by
serving as a convenient loading template for the required
applicator. Up to three universal bridges may be then placed
into each Cellas tank for electrophoresis.
Dysproteinaemia;
Albumin,
Alpha-1, Alpha-2,
Transferrin, C3 &
Gamma Globulin
Quantitation
Densitometer
CSLAPPS4
CSLAPPS6
A universal densitometer scanner with software capable
of reading 144 tests per minute is available for band
quantitation following post-electrophoretic staining.
Ordering Information - CellasGEL Applications Packages
equipped with bridges, applicator and clinical test kit
Incipient
Gammopathies
Ordering Information
CellasGEL Wet Membrane Applicators
Haemoglobinopathies
CSLAPPM8
Cellas Boxes
Hyper-lipidaemias
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Description
Volume
applied/
sample band
width
Compatible
Strip Size
CSLAPPS22
1x 2-specimen semi-micro
applicator
0.7μl / 7mm
2.5x14cm
CSLAPPS4SP
1x 4-specimen semi-micro
applicator
0.9μl / 9mm
5.7x14cm
CSLAPPS4
1x 4-specimen semi-micro
applicator
1.2μl / 9mm
5.7x14cm
CSLAPPS6
1x 6-specimen semi-micro
applicator
0.7μl / 7mm
5.7x14cm
CSLAPPM8
1x 8-sample micro applicator
0.3μl / 5mm
5.7x14cm
CellasGEL Wet Membrane Bridges and Densitometer
Paper Wicks
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Code
Bridges
CSLBDG8.5S 1x 8.5cm bridge for 1x 5.7x14cm or 2x 2.5x14cm CellasGEL
strips
CSLDENS
TurboScan Software Densitometer (excludes computer and
scanner)
CSLSCAN
Flatbed scanner for TurboScan software
Other applicators and bridges available – PLEASE ENQUIRE
44
I HORIZONTAL GEL SYSTEMS
CellasMEM Dry Membranes
Although CellasGEL cellulose acetate gels have a number of
advantages over traditional dry cellulose acetate membranes,
many manual systems and modern robotic platforms still
use dry cellulose acetate membranes, which are usually
supported on a plastic backing. Consequently, in order
to address this demand, Cleaver Scientific has developed
CellasMEM – a range of dry cellulose acetate membranes
supported on a Mylar film.
Each CellasMEM dry cellulose acetate membrane is
available in 25x76mm (CSLMEM257650), 60x76mm
(CSLMEM607625) and 94x76mm (CSLMEM947625) plate
sizes, and is compatible with the manual and automated
platforms of many leading and emerging brands within
the clinical electrophoresis market, including: Helena
Laboratories, Interlab and Seleo.
CellasMEM is supplied either as individual packs of
25 or 50 strips to perform manual assays for Serum
Protein (Dysproteinaemia; Incipient Gammopathies) and
Haemoglobin Analyses (Haemoglobinopathies such as
Thalassaemias and Sickle Cell Disorders). Please see
page 45 for Ordering Information.
HORIZONTAL GEL SYSTEMS I 45
semi-micro applicator is also available to load 6 samples,
each sample deposit 7-mm-wide and corresponding to 0.5µl
in volume.
Bridges
Adjustable bridges within the CELLAS tank render specialist
bridges unnecessary. Both CELLAS bridges may be
positioned either side of the central buffer partition within
the tank to produce the 76mm gap necessary to support
Helena-type membranes, while the 24cm width of the tank
accommodates either three 60x76mm or six 25x76mm
CellasMEM membranes per run. A dedicated bridge adaptor
(CSLMEMBA) is available for those users of non-Cleaver
cellulose acetate electrophoresis tanks that do not have
adjustable bridges.
features:
• CellasMEM dry cellulose acetate membranes
available in popular 25x76, 60x76 and 94x76mm
sizes
Applicators
• Ideal for use within the CSL-CELLAS system (Pg. 41)
The CellasMEM CSLMEMAPPM8 is an 8-sample
micro applicator designed for use with 60x76mm
(CSLMEM607625) and 25x76mm (CSLMEM257650)
CellasMEM dry plates. The applicator dispenses each sample
deposit as a thin band 5-mm-wide that is equivalent to
0.25µl in volume; and may load either one 60x76mm strip
or two 25x76mm strips (4 samples per strip) at a time.
By loading each sample as a tighter, but finer, band over a
wider front, the sample diffusion and saturation typical of
standard syringe loading methods is significantly reduced,
resulting in improved band quantitation. A CSLMEMAPPS6
• Robust Mylar film backing allow CellasMEM
Ordering Information
Code
Description
CellasMEM Dry Membrane Applicators
CSLMEMAPPM8
CSLMEMAPPS6
CellasMEM 8-sample micro applicator
CellasMEM 6-sample semi-micro applicator
CellasMEM Dry Membrane Bridge
CSLMEMBA
membranes to be used on automated platforms
• Infinite shelf life
• Compatible with manual and automated systems
from leading clinical electrophoresis brands, such as
Helena Laboratories, Interlab and Seleo.
• Most popular 60x76 and 25x76mm membranes
may be used with the tank, kit and accessories of the
manual Helena Titan 3 system; dedicated 8-sample
micro-applicator available (CSLMEMAPPM8)
• 30x76 & 60x76mm membranes available for
Genio Interlab small-and standard-sized automated
platforms; 70x62mm punched membranes available
for Interlab Microtech 648 automated platorm;
and 70x60mm punched membranes for the Seleo
Adalya, Exprime and Giant automated platforms.
• Other sizes available for older automated systems
still in use
• Densitometer software and scanner compatible
CellasMEM bridge adaptor for non-Cleaver
tanks
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Paper Wicks
Supplied in packs of 100 and available in 190x60mm and
220x40mm (WxL) sizes, CellasMEM disposable paper wicks
may be used respectively with standard Cellas MEM dry plates
and CellasMEM dry plates for Helena applications. To set
up the Cellas tank for use with dry plates, simply insert each
paper wick lengthwise within the tank pre-filled with buffer,
so that the buffer will become absorbed. Once absorbed, fold
over the top of each wick to make a support bridge, ensuring
the bottom edge of the wick is immersed within buffer and in
contact with the bottom of the tank, while the top edge rests
along the adjustable bridge. Repeat for the other bridge.
CellasMEM Membranes
CellasMEM membrane plates are available in many different
sizes and quantities ranging from the market-leading Helen
Titan 3 manual system to punched dry membrane plates
compatible with the strip-holders of automated systems
from Genio Interlab and SELEO. Also listed are CellasMEM
membranes for older systems - some of which are obsolescent
- that are still in use today.
CellasMEM Package Deals
Cleaver Scientific package deals provide a quick and
convenient solution for those users wishing to perform the
more popular Helena-type applications. The most basic
kit includes 25x76 and 60x76mm CellasMEM membranes,
8-sample micro applicator, and paper wicks, while the
complete version also contains a Cellas tank and new
nanoPAC-500 power supply.
Ordering Information
Code
Description
Paper Wicks
CSLMEMWICK
CellasMEM paper wicks 190x60mm, pack of 100
CellasMEM paper wicks 220x40mm, pack of 100;
suitable for Helena-type cellulose applications
CSLMEMWICKH with CSLMEM257650, CSLMEM607625,
CSLMEM577625, CSLMEM947625,
CSLMEM9413525 membranes
CellasMEM Package Deals
CSLMEMHKIT
CellasMEM Helena-Type Kit, includes 25x76mm
(CSLMEM257650) & 60x76mm (CSLMEM607625)
membranes; CSLMEMAPPM8 8-sample micro
applicator & CSLMEMWICKH 220x40mm paper
wicks.
CSLMEMH
COMPLETE
CellasMEM Helena-Type Workstation, includes
CSLMEMHKIT, CSL-CELLAS tanks & NANOPAC-500
(500V, 400mA, 120W) power supply (Pg 86 )
CellasMem Ordering Information (DRY MEMBRANES)
Code
Size (WxL)
Description
Compatible Manual System /
Automated Platform
CSLMEM257650
25x76mm
CellasMEM manual assay membrane, 50/pack
Helena Titan 3 system
CSLMEM607625
60x76mm
CellasMEM manual assay membrane, 25/pack
Helena Titan 3 system
CSLMEM577625
57x76mm
CellasMEM manual assay membrane, 25/pack
Helena Titan 3 system
CSLMEM947625
94x76mm
CellasMEM manual assay membrane, 25/pack
Helena Titan 3 system
CSLMEM9413525
94x135mm
CellasMEM manual assay membrane, 25/pack
Helena Titan 3 system
CSLMEM7660P25
76x60mm, punched
CellasMEM assay membrane 25/pack
SELEO AdaLya 24, Selvet 24,
Thera 72, Exprime , Giant
CSLMEM307625
30x76mm
CellasMEM assay membrane, 25/pack
Genio Interlab - small
CSLMEM607624
60x76mm
CellasMEM assay membrane, 24/pack
Genio Interlab - standard
CSLMEM7662P25
76x62mm, punched
CellasMEM assay membrane, 25/pack
Interlab 648 ISO, 648 PC
CSLMEM7413625
74x136mm
CellasMEM assay membrane, 25/pack
SAE - NT
CSLMEM7822725
78x227mm
CellasMEM assay membrane, 25/pack
SAE 500/600
CSLMEM8012525
80x125mm
CellasMEM assay membrane, 25/pack
Diafero Standard
CSLMEM2508025
280x80mm
CellasMEM assay membrane, 25/pack
Diafero Extra
CSLMEM8022525
80x225mm
CellasMEM assay membrane, 25/pack
Cliniphor
CSLMEM762325
76x23mm
CellasMEM assay membrane, 25/pack
Saechem
CSLMEM678930
67x89mm
CellasMEM assay membrane, 30/pack
Smart
CSLMEM7618025
76x180mm
CellasMEM assay membrane, 25/pack
Pragma
CSLMEM7621025
76x210mm
CellasMEM assay membrane, 25/pack
Megaphore
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
THE OMNIPAGE RANGE AND WAVE MAXI – Key Features
VERTICAL
GEL
SYSTEMS
The omniPAGE mini, mini wide and new VS20WAVE systems have been designed by scientists with the laboratory scientist in mind.
Each system comprises a modular tank design with optional dedicated inserts available for PAGE, blotting and capillary gel isoelectric focusing (IEF), potentially allowing the user to perform up to three techniques in a day.
Tank and Lid Design
High quality injection moulded construction and durable leak-proof
design for complete safety and longevity.
Elongated thumb locators –
colour-coded to prevent
polarity reversal and
facilitate ‘easy-off’ lid
removal.
Corrosion-resistant, 99.99% pure platinum electrodes.
Electrical safety – lid removal immediately disconnects
power to the running insert and lower buffer chamber to allow
entirely safe access to the gel.
Vertical electrophoresis utilises potent protein and nucleic
acid analytical tools for applications within all aspects of
life science research, ranging from purity determination to
analysis of complex protein lysates. Accordingly, Cleaver
Scientific’s remit is simple: to provide a comprehensive range
of vertical electrophoresis systems – complete with tanks,
inserts and reagents – to fulfil a variety of applications and
techniques in different gel sizes and throughputs.
COMBS AND SPACERS
Reversible combs also serve as
loading indicators to aid pipettewell alignment,
preventing sample loading errors
– simply invert the comb prior
to loading to reveal a loading
template that sits conveniently
above the newly formed sample
wells
Combs and spacers are injection moulded for consistency and
‘clean’ well formation, and are available in 4 thicknesses and
colour-coded:
• White – 1mm supplied as standard
• Black – 0.75mm for tightly resolved bands
• Red – 1.5mm to maximise sample volume
• Blue – 2mm to maximise sample volume
Black and white combs recommended for high resolution
publication quality data; red and blue to scale-up volumes for
preparatory techniques.
CASTING AND RUNNING
Dual purpose PAGE inserts eliminate timeconsuming transfer of glass plates between
separate casting and running modules.
Cam-Pin caster locks PAGE insert onto the ultra-soft
silicone mat within casting base to provide a leak-free
seal.
Selection Guide
48-49
omniPAGE Mini
50-51
Octi- Page
OMNIPAGE MINI
Flat, ultra-soft moulded gasket acts
in tandem with a unique single-piece
pressure clamping frame to facilitate
even pressure distribution to minimise
gel compression; gasket reversible for
Bio-Rad compatibility.
Unique sliding-clamp
technology within PAGE
insert allows rapid set
up of handcast and
precast gels.
Gel release tool
quickly releases
glass plates from
PAGE insert.
WAVE
Resting slots allow gel clamps to sit conveniently
out of the way for hindrance-free loading of gel
cassettes.
Novel vertical screw-clamp technology utilises only 4 screws for
faster set up compared to traditional large-format systems.
Sliding gel-clamps available in two thicknesses to
accommodate single- and double-gel cassettes.
Ergonomic ‘wave’ design of PAGE insert provides convenient finger
grips for easy handling.
2-D AND BLOTTING OPTIONS (Available as standalone add-ons or as part of fully integrated electrophoresis systems for multiple
electrophoresis techniques –e.g. CVS10CBS [PAGE & blotting], CVS10C2DS [IEF & PAGE], CVS10CES [IEF, PAGE, blotting])
52
omniPAGE Mini Wide
54-55
VS20WAVE Maxi Vertical
56-57
Complete Modular Systems
58-59
2-D Systems
60-61
Isoelectric Focusing and second
stage 2-D electrophoresis
62-64
Vertical Electrophoresis Packages
66-67
Large Format Vertical
68-69
Denaturing Gradient Gel Electrophoresis
70-71
Modular blotting inserts for omniPAGE MINI, MINI WIDE and WAVE systems use the tank and lid of the
corresponding PAGE system to perform fast, high-quality blotting of a maximum 4 gels; high-intensity plate
electrode option available for the omniPAGE MINI and WAVE.
RELATED PRODUCTS
Capillary tube gel inserts utilise the same tank and lid of corresponding PAGE system for reproducible 2-D
electrophoresis; isoelectric focusing (IEF) of up to 10 capillary tube gels may be completed in as little as 3.5
hours. Optional IPG converter kits modify PAGE gels to accommodate commercial IPG strips.
ACCESSORIES
Robust scientific grade glass plates: 2mm thick omniPAGE MINI; 4mm thick omniPAGE MINI WIDE and WAVE.
Notched glass plates with bonded spacer option may be used with standard plain glass plate with 1mm bonded spacer and notched
glass plates to double gel capacity of the PAGE insert, allowing a maximum of 4 gels to be run (supplied as standard with CVS10TETRAD systems).
Power Supplies PAGEs 82-89
Gel
Documentation PAGEs 90-109
Thermal Cyclers PAGE 123
Buffers and
Markers PAGE 142
Cool packs – simple to use cooling pack systems ensure enhanced resolution without costly and time-consuming additional
equipment. Simply pre-chill in a freezer and place within the gel tank; cool packs also reduce buffer volume.
Power cables with 4mm connectors compatible with most modern
low-to-medium voltage power supplies; CE compliant. High voltage cables
with 2mm connectors and adaptors available for complete power supply
compatibility
Detachable inner cooling coil connects to the laboratory water supply or
a recirculating chiller to provide uniform smile-free electrophoresis, while
allowing runs to be performed at higher voltage.
Gradient mixers and multicasters available to cast multiple mini vertical
gradient gels.
Glass plate racks for safe drying and storage of glass plates.
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
48
I vertical electrophoresis systems
vertical electrophoresis systems I 49
Selection Guide
Advantages
OmniPAGE Mini System
OCTIPAGE MINI SYSTEM
Run 1-4 handcast gels, and up to 2 precast gels in mini
format
Sliding clamp assembly ensures fast set up times and leakfree operation
Insert for both gel casting and running eliminating timeconsuming transfer of fragile gels
Small compact unit offering ultimate economy in buffer
and reagent consumption
Modular design for rapid turnaround of data, allowing
PAGE, 2-D and blotting to be completed within a working
day
Ideal for discovery projects and evaluation of sample
preparation conditions
Run 1-8 handcast gels and up to 4 precast gels
Sliding clamp assembly ensures fast set up times and leakfree operation
Insert for both gel casting and running eliminating timeconsuming transfer of fragile gels
Small compact unit offering ultimate economy in buffer
and reagent consumption
Modular design for rapid turnaround of data, allowing
PAGE, 2-D and blotting to be completed within a working
day
Ideal for discovery projects and evaluation of sample
preparation conditions
OmniPAGE Mini WIDE System New VS20WAVE Maxi System
Commercial 10x10cm and 10x8cm (W x H) precast gels:
e.g. IDGel™, SERVA, Thermo and Invitrogen, etc.
Commercial 10x10cm and 10x8cm (W x H) precast gels:
e.g. IDGel™, SERVA, Thermo and Invitrogen
Handcast
OmniPAGE VS10 glass plates with or without bonded
spacers for handcast gels
OmniPAGE VS10 glass plates with or without bonded
spacers for handcast gels
Electrophoresis Systems
Innovative vertical screw-clamp technology exerts
uniform pressure along the height of the glass
plates, facilitating a leak-free seal, without gel
compression and bowing, ensuring even sample
migration
Even pressure screw system
Advantages
Combine pI (isoelectric point) separation with
speed by resolving
2x 7cm IPG strips or 2x 8cm capillary tube gels
per gel using special 2-D gel combs and plates
2-gel systems
CVS10PRE (Pg 50)
4-gel system
CVS10OCTIPAGE-PRE (Pg 52)
Fewer screws compared to traditional formats
resulting in rapid set up times
Optional blotting insert supplied with additional
tank and lid for a dedicated 4-gel blotting system;
rapid transfer plate electrodes available
Detachable cooling core for fast, smile-free
electrophoresis
Seamless injection moulded construction free of
potential leakage-prone glue joins
Capacity to run 1-4 18cm capillary tube gels or
IPG strips in second dimension; optional 2-D
module
Compatible Gel Formats
Handcast
VS10W plain and notched glass plates with or
without bonded spacers for handcast gels
VS20 plain and notched glass plates with or
without bonded spacers for handcast gels
Electrophoresis Systems
Standard
CVS10D (Pg 50)
Precast (tank, lid and running insert only)
CVS10DSYS (Pg 50)
CVS10OCTIPAGE1 (Pg 52)
CVS10DSYS-CU (Pg 50)
Vertical system and optional power supply combination
2-gel
8-gel
CVS10OCTIPAGE-PRENANO500
CVS10PRE-CS300 (Pg 66)
Precast
CVS10OCTIPAGE-CS300
CVS10DSYS-CS300 (Pg 66)
Handcast
CVS10OCTIPAGECBS-CS300
CVS10CBS-CS300 (Pg 66)
Handcast with blotting insert
Vertical system and optional power supply combination
Tetrad Packages
4-gel
8-gel
CVS10OCTIPAGE1
CVS10TETRAD1 (Pg 66)
Handcast
(with blotting option)
CVS10OCTIPAGE1CBS
CVS10TETRAD1CBS (Pg 66)
(with power supply)
CVS10OCTIPAGE1-CS300
CVS10TETRAD1-CS300 (Pg 66)
(with power supply and blotting option CVS10TETRAD1CBS-CS300 (Pg 66)
CVS10OCTIPAGE1CBS-CS300
(with high specification power supply) CVS10TETRAD1-CS3AMP (Pg 66)
CVS10OCTIPAGE1-NANO500
(with high current power supply and
CVS10OCTIPAGE1CBS-CS3AMP
CVS10TETRAD1CBS-CS3AMP (Pg 66)
blotting option)
Combination Package
Available Gel Thicknesses
Run 1-2 handcast gels; perfect for users with
>20 samples to compare and resolve
Ability to perform three techniques in a day: IEF,
PAGE and blotting
Precast
Active Gel Dimensions (w x h)
Runs 1-4 large format gels at maximum resolution
Screw assembly and moveable gel stops prevent
gel leakage
Compatible Gel Formats
Standard
Precast (tank, lid and running
insert only)
Tapecast (includes glass plates)
Handcast
(with glass plates and caster)
(with extra casting stand and
plates to run 2 gels in tank, while
casting 2 simultaneously)
Mini wide format effectively allows 2 mini gels to
be compared within a single gel for
gel-to-gel reproducibility
8 x 8.5cm
8 x 8.5cm
0.5, 0.75, 1, 1.5 & 2mm
Tapecast (includes glass plates)
VS10WD (Pg 55)
Handcast
(with glass plates and caster)
VS10WDSYS (Pg 55)
VS20WAVESYS (Pg 57)
VS10WDSYS-CU (Pg 55)
VS20WAVESYS-CU (Pg 57)
(with extra casting stand and plates to run 2 gels in
tank, while casting 2 simultaneously)
Combination Package
Handcast
Handcast with blotting insert
Tetrad Packages
WAVETETRAD1
WAVETETRAD1CBS
WAVETETRAD1-CS500
(with power supply and blotting option
(with high current power supply)
(with high current power supply and blotting option)
WAVETETRAD1CBS-CS500
WAVETETRAD1-CS3AMP
WAVETETRAD1CBS-CS3AMP
Active Gel Dimensions (w x h)
18 x 8cm
Available Gel Thicknesses
OmniPAGE Mini CVS10CBS (PAGE & Blotting, Pg 74) and
CVS10CES (PAGE, Blotting & 2-D, Pg 58)
Compatible Electroblotting
Transfer Systems Systems
Integrated modular
Standalone
Wet/tank transfer
SB10 and EBM10 4- and 5-blot transfer systems
(Pg 78)
Standalone
Wet/tank transfer
Semi-dry
SD10 10x10cm and SD20 20x20cm for 1x and 4x blots
(Pg 80)
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
4-gel vertical system and optional power supply combination
Handcast
(with blotting option)
(with power supply)
Compatible Electroblotting
Transfer Systems Systems
Integrated modular
SD10 10x10cm and SD20 20x20cm for 1x and 4x blots
(Pg 80)
2-gel vertical system and optional power supply combination
VS10WDSYS-CS300 (Pg 67)
VS10WCBS-CS300 (Pg 67)
Semi-dry
16 x 17.5cm
0.5, 0.75, 1, 1.5 & 2mm
OmniPAGE Mini Wide VS10WCBS (PAGE &
Blotting, Pg 75) and VS10WCES (PAGE, Blotting
& 2-D, Pg 58)
SB10W and EBM20 3- and 5-blot transfer
systems (Pg 78)
SD20 20x20cm for 2x blots (Pg 80)
VS20CBS (PAGE & Blotting, Pg 76)
SB20 and EBM20 3- and 5-blot transfer systems
(Pg 78)
SD20 20x20cm, SD33 33x45cm, and SD50
20x50cm for 1-3 blots (Pg 80)
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
50
I vertical electrophoresis systems
vertical electrophoresis systems I 51
ORDERING INFORMATION
CVS10DSYS
omniPAGE Mini, 10 x 10cm Dual, 2 sets of Glass
Plates, 1mm thick bonded Spacers, 2 x 12 sample,
1mm thick combs including caster.
CLAMP VERSION
CVS10D
omniPAGE Mini, 10 x 10cm Dual, 2 sets of Glass
Plates, 1mm thick bonded Spacers, 2 x 12 sample,
1mm thick combs. CLAMP VERSION
CVS10PRE
omniPAGE Mini, 10 x 10cm Dual. No accessories.
CLAMP VERSION
omniPAGE Mini, 10 x 10cm Dual, 2 sets of Glass
Plates, 1mm thick bonded Spacers, 2 x 12 sample,
1mm thick combs including caster.
CLAMP VERSION, External casting upstand
CVS10DSYS-CU
features:
High Throughput Capability
• Double gel capacity and run
OmniPAGE mini vertical component parts
CVS10EXCASTER
External Casting Upstand - No Casting Base
CVS10EXCASTERSYS
External Casting System - Upstand + Base
1. Lid
4. P
lain glass plates with
bonded spacers
7. G
el release tool
VS10DCAST
10 x 10cm Casting Base
2. Tank
5. PAGE insert
8. Mini cool pack
VS10DCASTM
Replacement Silicone Mat for 10 x 10cm Casting
Base
3. Combs
6. Caster
9. Notched glass plates
CVS10DIRM
Inner Running Module
VS10ICB
Mini Cooling Pack
VS10NG
10 x 10cm Notched Glass Plates 2mm thick (pk/2)
VS10PG
10 x 10cm Plain Glass Plates 2mm thick (pk/2)
Code
Description
VS10NGS0.75
10 x 10cm Notched Glass Plates with 0.75mm
Bonded Spacers (pk/2)
10 x 10cm Plain Glass Plates with 0.75mm
Bonded Spacers (pk/2)
VS10-1-0.75
Comb 1 Prep, 1 Marker, 0.75mm thick
500μl
VS10PGS0.75
VS10-5-0.75
Comb 5 sample, 0.75mm thick
70μl
VS10NGS1
10 x 10cm Notched Glass Plates with 1mm
Bonded Spacers (pk/2)
VS10PGS1
10 x 10cm Plain Glass Plates with 1mm Bonded
Spacers (pk/2)
VS10NGS1.5
a maximum of 4 gels within
an hour, using a combination
of plain and notched glass
plates with bonded spacers in
between that correspond to
your chosen gel thickness
Rapid Casting
• PAGE insert, used for both gel
For further details see the OmniPAGE™ Key Features Section on page 46.
COMB SPECIFICATIONS
Sample
Volume per
well
casting and running, eliminates
time-consuming transfer of
potentially fragile glass plates
between separate casting and
running modules
• Cast and run in 4 easy steps
using unique sliding clamp
technology…
Innovative Loading
• Reversible combs also serving
VS10-8MC-0.75 Comb 8 sample MC, 0.75mm thick
40μl
VS10-9-0.75
Comb 9 sample, 0.75mm thick
35μl
VS10-10-0.75
Comb 10 sample, 0.75mm thick
30μl
10 x 10cm Notched Glass Plates with 1.5mm
Bonded Spacers (pk/2)
VS10-12-0.75
Comb 12 sample, 0.75mm thick
25μl
VS10PGS1.5
10 x 10cm Plain Glass Plates with 1.5mm Bonded
Spacers (pk/2)
VS1016MC-0.75
Comb 16 sample MC, 0.75mm thick
20μl
edicated Modules for Different
D
Applications
VS10NGS2
10 x 10cm Notched Glass Plates with 2mm
Bonded Spacers (pk/2)
VS10-20-0.75
Comb 20 sample, 0.75mm thick
15μl
• Interchangeable modular
VS10-1-1
Comb 1 Prep, 1 Marker, 1mm thick
650μl
VS10-5-1
Comb 5 sample, 1mm thick
100μl
VS10-8MC-1
Comb 8 sample MC, 1mm thick
60μl
VS10-9-1
Comb 9 sample, 1mm thick
50μl
VS10-10-1
Comb 10 sample, 1mm thick
40μl
VS10-12-1
Comb 12 sample, 1mm thick
35μl
VS10-16MC-1
Comb 16 sample MC, 1mm thick
25μl
VS10-20-1
Comb 20 sample, 1mm thick
VS10-1-1.5
Comb 1 Prep, 1 Marker, 1.5mm thick
1000μl
VS10-5-1.5
Comb 5 sample, 1.5mm thick
140μl
VS10PGS2
10 x 10cm Plain Glass Plates with 2mm Bonded
Spacers (pk/2)
VS10DP
Dummy Plate, 10 x 10cm
VS10S0.75
10cm Spacers - 0.75mm (pk/2)
VS10S1
10cm Spacers - 1mm thick (pk/2)
VS10S1.5
10cm Spacers - 1.5mm thick (pk/2)
VS10S2
10cm Spacers - 2mm thick (pk/2)
RPW-0.2
Replacement Platinum Wire - 0.2mm, 50cm
SPECIFICATIONs
Number of
gels
1-4
Total buffer
Volume for 2 gels
Min: 250mL
Max: 1200mL
Precast gel
compatibility
IDGel™,
Novex®,
SERVAGel™,
Thermo
Precise Pierce
Protein Gel
Total buffer
volume for 4 gels
Min: 250mL
Max: 1200mL
Using VS10
glass plates
Standard run
time for
SDS-PAGE
1-2 hours at from
90-225V
Recommended
power supplies
NanoPAC-300 (Pg 86);
NanoPAC-500 (Pg 86);
CS-300V (Pg 87);
CS-3AMP for blotting
(Pg 88)
Handcast gels
10x10x0.2cm
Plate
dimensions (w
x h x t)
Gel
Dimensions
(w x h)
8x8.5cm
Unit Dimensions
(w x d x h) Weight 19x13x15cm
1.8Kg
20μl
VS10-8MC-1.5
Comb 8 sample MC, 1.5mm thick
80μl
VS10-9-1.5
Comb 9 sample, 1.5mm thick
70μl
VS10-10-1.5
Comb 10 sample, 1.5mm thick
30μl
VS10-12-1.5
Comb 12 sample, 1.5mm thick
50μl
VS10-16MC-1.5 Comb 16 sample MC, 1.5mm thick
40μl
VS10-20-1.5
30μl
Comb 20 sample, 1.5mm thick
VS10-1-2
Comb 1 Prep, 1 Marker, 2mm thick
1300μl
VS10-5-2
Comb 5 sample, 2mm thick
200μl
VS10-8MC-2
Comb 8 sample MC, 2mm thick
120μl
VS10-9-2
Comb 9 sample, 2mm thick
100μl
VS10-10-2
Comb 10 sample, 2mm thick
80μl
VS10-12-2
Comb 12 sample, 2mm thick
70μl
VS10-16MC-2
Comb 16 sample MC, 2mm thick
50μl
VS10-20-2
Comb 20 sample, 2mm thick
40μl
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
as loading indicators aid
pipette-well alignment to
prevent sample loading errors
inserts for slab gels,
2-D electrophoresis and
electroblotting allow the user
to switch quickly and easily
between electrophoresis
techniques, using the same,
single universal buffer tank
and lid.
Our modular system
configurations are as follows: -
Cast and run in 4 easy steps
Check out video at www.cleaverscientific.com for more details..
omniPage Mini
Cleaver Scientific Mini Vertical systems
are used primarily for protein but also
nucleic acid electrophoresis techniques.
By combining both functionality and
ease of use, the omniPAGE CVS10
systems set the bench mark for simple,
versatile vertical mini gel electrophoresis. Each omniPAGE electrophoresis
system can accommodate up to 4
handcast gels and 2 commercial precast
gels to provide complete flexibility for
individual research needs, while unique
sliding clamp technology within the
PAGE insert facilitates fast, intuitive
leak-free casting.
With a trouble-free set up and consistent performance, the omniPAGE
Mini Vertical systems are perfect for
today’s laboratories where the ability to
generate reproducible results quickly is
essential.
- Electroblotting of 1 to 4
PAGE gels (Pg 74)
- 2-D Electrophoresis using
capillary tube gel isoelectric
focusing & PAGE (Pg 60)
- Complete – combine
electroblotting and 2-D
electrophoresis (Pg 58)
Typical Applications
Ideal for the separation of proteins in
poly acrilamide gels.
The omniPAGE Mini blotting insert is available in traditional wire
electrode format or with rapid high-intensity plate electrodes
(Pg 79) to transfer up to 4 and 2 gels respectively
• Ideal for discovery projects and
evaluation of sample preparation
conditions.
• PAGE, 2-D electrophoresis and
blotting
The omniPAGE Mini capillary
tube gel insert may be used for
IEF of up to 10 capillary tube
gels in as little as 3.5 hours
(Pg 60)
• Gel purification of low molecular
weight nucleic acid fragments
• To learn more, please visit
www.cleaverscientific.com to see
our technical video
52
I vertical electrophoresis systems
vertical electrophoresis systems I 53
features:
• Compare data from up to eight independent gels
run within two PAGE inserts
• Unique sliding clamp technology facilitates rapid
interchange between casting and running inserts
• Run eight gels in the tank whilst casting another
Octi-Page
Octi-Page is Cleaver Scientific’s solution for high throughput
electrophoresis of 10x10cm mini-gels. Based on the 4-gel
capacity of the CVS10TETRAD system, the Octi-Page takes
electrophoresis one step further by allowing eight single gels
to be run within two individual CVS10DIRM running inserts.
By using the standard CVS10 mini-vertical plain glass plate
with bonded spacer (VS10PGS1) and notched glass plate
(VS10NG) configuration, the researcher not only benefits
from being able to compare data between single gels run
within the same tank but is also free of the complications that
sometimes occur when opening and processing double-gel
cassettes or triple-plate sandwiches.
Two casting bases are supplied to cast up to 8 gels within two
CVS10DIRM running inserts which are then transferred to the
tank for electrophoresis. While electrophoresis is underway,
time may be then spent casting another 8 gels using the two
casting upstands (CVS10EXCAST) in tandem with the casting
bases. Once the first electrophoresis run is completed, the
CVS10 mini-vertical’s unique sliding clamp technology allows
the newly cast gels to be switched quickly and easily from
the casting upstands to the running inserts, keeping the tank
permanently in use for maximum throughput.
eight gels simultaneously, to keep the tank in
permanent use and maximise throughput
• Run eight, Blot eight! - optional blotting insert
has double the transfer capacity of the market
leader, eliminating bottlenecks between PAGE and
blotting
• Suitable for precast gels, and available in a
selection of customised packages with optional
blotting insert and specialist power supply
The optional VS10BI blotting insert also fits comfortably
within the tank to blot all eight PAGE gels, while two reusable
freezer packs provide enhanced low-cost cooling to maintain
protein stability, particularly during native transfers. The
Octi-Page may be supplied pre-configured for commercial
precast gels, or as a complete package with power supply and
blotting insert.
TECHNICAL SPECIFICATION
Gel dimensions (w x l)
10 x 8cm
Unit dimensions (w x l x h)
15 x 15 x 4cm
Max. samplecapacity
40 samples
Buffer volume
50ml
Combs available:
No. of samples
Thicknesses
1, 4, 8, 12, 16, 20
1, 1.5mm
ORDERING INFORMATION
CVS10OCTIPAGE-PRE
Octi-Page Mini-Protein Electrophoresis Package for
commercial precast gels, includes 2x CVS10DIRM, 1x
VS10DP, tank, lid and power cables
CVS10OCTIPAGE-PRENANO500
Octi-Page Mini-Protein Electrophoresis Package
for commercial precast gels, includes CVS10OCTIPAGE-PRE and nanoPAC-500 power supply
CVS10OCTIPAGE1
Octi-Page Mini-Protein Electrophoresis Package for
1mm self-cast gels, includes: 2x CVS10DIRM, 1x VS10DP, 4x VS10-12-1, 4x VS10PGS1, 4x VS10NG, 4x
VS10NGS1, 2x CVS10EXCASTER and 2x VS10DCAST
CVS10OCTIPAGE1CBS
Octi-Page Mini-Protein Electrophoresis Package for
1mm self-cast gels with interchangeable 4-blot insert,
includes: CVS10OCTIPAGE1 plus VS10BI blotting insert
Octi-Page Mini-Protein Electrophoresis Package for
CVS10OCTI1mm self-cast gels with Mini power supply, includes:
PAGE1-NANO500
CVS10OCTIPAGE1 plus nanoPAC-500 power supply
Multiple
Minigel Casting
Advance casting of multiple mini gels can help to achieve
consistent results between runs. These multiple gel casting
systems are tailored for use with omniPAGE mini vertical
electrophoresis units; with three models of 6, 12 and 24 gel
capacities. Fewer gels can be poured if required using the
acrylic saver blocks supplied with each system.
The fixed hinged clamps allows the gel sandwich to be adjusted to the correct pressure irrespective of the number or
the thickness of gels being poured. Separation sheets allow
the easy separation of the glass plates after pouring.
features:
• Integral levelling feet and level bubble
CVS10OCTIPAGE1-CS300
Octi-Page with standard Midi power supply option, includes: CVS10OCTIPAGE1 plus CS-300V power supply
Octi-Page with Midi power supply and interchangeable
CVS10OCTI4-blot module, includes: CVS10OCTIPAGE1CBS and
PAGE1CBS-CS300
CS-300V
CVS10OCTIPAGE1-CS3AMP
Octi-Page with programmable high current Maxi
power supply option, includes CVS10OCTIPAGE1 and
CS3AMP
CVS10OCPAGE1CBSCS3AMP
Octi-Page with programmable high current Maxi power supply and interchangeable 4-blot module, includes:
CVS10OCTIPAGE1CBS and CS-3AMP
• Standard and gradient gels can
be poured
• Choice of 6, 12 or 24 gel casting models
ORDERING INFORMATION
CSL-6CAST
6 gel caster for 8 x 10cm or 10 x 10cm gels
CSL-12CAST
12 gel caster for 8 x 10cm or 10 x 10cm gels
CSL-24CAST
24 gel caster for 8 x 10cm or 10 x 10cm gels
Change 1 for your required spacer and comb thickness i.e. 1.5mm
Typical Applications
Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE) and
electroblotting. 2-D gel electrophoresis (requires VS10DCI tube
gel insert). Rapid screening of new samples, and evaluation of
sample preparation conditions
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Typical Applications
Casting of multiple 10 x 10cm gels up to 24.
Casting Gradient gels
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
54
I vertical electrophoresis systems
ORDERING INFORMATION
VS10WD
omniPAGE Mini Wide, 20 x 10cm Dual, 2 sets
of Glass Plates with 1mm thick bonded Spacers,
2 x 24 sample, 1mm thick combs, cooling pack
VS10WDSYS
omniPAGE Mini Wide, 20 x 10cm Dual, 2 sets
of Glass Plates with 1mm thick bonded Spacers,
2 x 24 sample, 1mm thick combs, cooling pack
including caster
VS10WDSYS-CU
omniPAGE Mini Wide, 20 x 10cm Dual, 2 sets
of Glass Plates, 1mm thick bonded Spacers, 2 x
24 sample, 1mm thick combs including caster,
External casting upstand
VS10WEXCASTER
VS10W External Casting Upstand - No Casting
Base
VS20DCAST
20 x 10cm Casting Base
VS20DCASTM
Replacement Silicone Mat for 20 x 10cm
Casting Base
VS10WDIRM
Inner Running Module
VS20-x -LG
Loading guides for omniPAGE mini combs, x =
comb well number
VS10WNG
20 x 10cm Notched Glass Plates 4mm thick
(pk/2)
VS10WPG
20 x 10cm Plain Glass Plates 4mm thick (pk/2)
VS10WNGS0.75
20 x 10cm Notched Glass Plates with 0.75mm
Bonded Spacers (pk/2)
VS10WPGSO.75
20 x 10cm Plain Glass Plates with 0.75mm
Bonded Spacers (pk/2)
VS10WNGS1
20 x 10cm Notched Glass Plates with 1mm
Bonded Spacers (pk/2)
VS10WPGS1
20 x 10cm Plain Glass Plates with 1mm Bonded
Spacers (pk/2)
VS10WPGS1.5
20 x 10cm Plain Glass Plates with 1.5mm
Bonded Spacers (pk/2)
VS10WPGS2
20 x 10cm Plain Glass Plates with 2mm Bonded
Spacers (pk/2)
VS10WDP
Dummy Plate, 20 x 10cm
RPW-0.2
Replacement Platinum Wire - 0.2mm, 50cm
VS20ICB
Maxi Cooling PackVS10WEXCASTER
vertical electrophoresis systems I 55
TECHNICAL SPECIFICATIONS
Plate dimensions,
Gel dimensions (w x l)
20 x 10cm
18 x 8cm
Unit dimensions (w
x l x h)
26 x 16 x 16cm
Max. sample capacity
192 samples, 48 samples per gel
Buffer volume
Inner Chamber: 250ml
Outer Tank: Min. 600ml – Max. 2800ml
Combs available:
No. of samples
Thicknesses
NEW
IMPRO
WAVE VED
DES
RUNNIN IGN
MODU G
LE!
1, 5, 10, 18MC, 24, 30, 36MC, 48
0.75, 1, 1.5, 2mm
features:
• Offers the capacity
of two mini gels on
a single gel
• Rapid gel casting
and loading
• Optional low or
high buffer volumes
• Rapid set up cooling
The omniPAGE mini wide vertical
gel unit with an active gel width of
18cm effectively allows double the
number of samples to be resolved as
the omniPAGE mini unit. This allows
consistency of sample comparison
on a single gel and is designed for
those with greater than 20 samples to
compare and resolve.
Simple set up using ultra soft silicone
seals guarantees trouble free glass plate
loading and gel casting. Dual gaskets
on the gel running insert along with
notched and plain glass plates ensure
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
omniPage mini wide
Mini SDS PAGE, Native PAGE,
Gradient, Second dimension and
Nucleic acid separations
leak proof gel running. Rapid set up
cooling retains resolution in extended
separations and also saves on buffer
volume without affecting run quality.
4mm thick glass plates reduce
breakage and have bonded spacers for
added convenience. A wide range of
accessories is available allowing many
techniques to be performed using the
same unit. Prep combs can be used to
maximise sample loading and recovery.
Accessory electroblotting and tube gel
modules are available which use the
same outer tank and lid.
56
I vertical electrophoresis systems
vertical electrophoresis systems I 57
ORDERING INFORMATION
VS20WAVE Maxi, 20 x 20cm Dual with Glass
Plates with bonded 1mm thick spacers, 2x 24
sample combs, cooling coil, dummy plate and
Casting Base
VS20WAVESYS
COMB SPECIFICATIONS
Sample
Volume per
well
Code
Description
VS20WAVE Maxi, 20 x 20cm Dual, 2 sets of Glass
Plates, 1mm thick bonded Spacers, 2 x 24 sample,
1mm thick combs, cooling coil, dummy plate;
includes caster and External casting upstand
VS20-1-0.75
Comb 1 Prep, 1 Marker, 0.75mm thick
1100μl
VS20-5-0.75
Comb 5 sample, 0.75mm thick
160μl
VS20WAVE-EC
VS20WAVE External Casting Stand - No Casting
Base
VS20-10-0.75
Comb 10 sample, 0.75mm thick
80μl
VS20DCAST
20 x 20cm Dual Casting Base
VS2018MC-0.75
Comb 18 sample MC, 0.75mm thick
40μl
VS20DCASTM
Replacement Silicone Mat for 20 x 20cm Casting
Base
VS20-24-0.75
Comb 24 sample, 0.75mm thick
30μl
VS20WAVEDIRM
PAGE insert
VS20-30-0.75
Comb 30 sample, 0.75mm thick
25μl
VS20WAVE-CC
Detachable Cooling Coil
VS2036MC-0.75
Comb 36 sample MC, 0.75mm thick
20μl
VS20-x –LG
Loading guides for omniPAGE maxi combs, x =
comb well number
VS20-48-0.75
Comb 48 sample, 0.75mm thick
15μl
VS20NG
20 x 20cm Notched Glass Plates 4mm thick (pk/2)
VS20-1-1
Comb 1 Prep, 1 Marker, 1mm thick
1500μl
VS20PG
20 x 20cm Plain Glass Plates 4mm thick (pk/2)
VS20-5-1
Comb 5 sample, 1mm thick
200μl
VS20NGS0.75
20 x 20cm Notched Glass Plates with 0.75mm
Bonded Spacers (pk/2)
VS20-10-1
Comb 10 sample, 1mm thick
100μl
20 x 20cm Plain Glass Plates with 0.75mm
Bonded Spacers (pk/2)
VS20-18MC-1
Comb 18 sample MC, 1mm thick
50μl
VS20PGS0.75
VS20-24-1
Comb 24 sample, 1mm thick
40μl
VS20NGS1
20 x 20cm Notched Glass Plates with 1mm
Bonded Spacers (pk/2)
VS20-30-1
Comb 30 sample, 1mm thick
35μl
VS20PGS1
20 x 20cm Plain Glass Plates with 1mm Bonded
Spacers (pk/2)
VS20-36MC-1
Comb 36 sample MC, 1mm thick
25μl
VS20PGS1.5
20 x 20cm Plain Glass Plates with 1.5mm Bonded
Spacers (pk/2)
VS20-48-1
Comb 48 sample, 1mm thick
20μl
20 x 20cm Plain Glass Plates with 2mm Bonded
Spacers (pk/2)
VS20-1-1.5
Comb 1 Prep, 1 Marker, 1.5mm thick
2200μl
VS20PGS2
VS20-5-1.5
Comb 5 sample, 1.5mm thick
320μl
VS20DP
Dummy Plate, 20 x 20cm
VS20-10-1.5
Comb 10 sample, 1.5mm thick
160μl
VS20S0.75
20cm Spacers - 0.75mm (pk/2)
VS20-18MC-1.5
Comb 18 sample MC, 1.5mm thick
80μl
VS20S1
20cm Spacers - 1mm thick (pk/2)
VS20-24-1.5
Comb 24 sample, 1.5mm thick
60μl
VS20S1.5
20cm Spacers - 1.5mm thick (pk/2)
VS20-30-1.5
Comb 30 sample, 1.5mm thick
50μl
20cm Spacers - 2mm thick (pk/2)
VS20-36MC-1.5
Comb 36 sample MC, 1.5mm thick
40μl
IEF Conversion for 18cm IPG strips and tube gels,
includes: 1 set of plain glass plates with bonded
spacers, 0.6x20cm (WxH); and 2x 2-D combs
with one 3.5mm marker lane and one 18cm
preparatory well
VS20-48-1.5
Comb 48 sample, 1.5mm thick
30μl
VS20-1-2
Comb 1 Prep, 1 Marker, 2mm thick
3000μl
VS20-5-2
Comb 5 sample, 2mm thick
400μl
Replacement Platinum Wire - 0.2mm, 100cm
VS20-10-2
Comb 10 sample, 2mm thick
200μl
VS20-18MC-2
Comb 18 sample MC, 2mm thick
100μl
VS20-24-2
Comb 24 sample, 2mm thick
80μl
VS20-30-2
Comb 30 sample, 2mm thick
70μl
VS20-36MC-2
Comb 36 sample MC, 2mm thick
50μl
VS20-48-2
Comb 48 sample, 2mm thick
40μl
VS20WAVESYS-CU
VS20S2
VS20WAVE-IEFKIT
RPW-0.2100
MC=multichannel pipette compatible
SPECIFICATIONs
Number of
gels
Handcast gels
1-4
Using VS20 glass
plates and combs
Total Volume Inner
Buffer Chamber
640mL
Total buffer Volume
for 2 gels
5.3L
Total buffer volume
for 4 gels
4.8L
Standard run time for
SDS-PAGE
Without Cooling
With Cooling
Standard
Spacer
Dimensions
(w x h)
Recommended power EV233 for IEF (Pg 89);
supplies
CS-500V for PAGE
(Pg 88); CS-3AMP for
blotting (Pg 88)
IPG Spacer
0.6x20cm
Dimensions (w
x h)
Unit Dimensions (w
x d x h)
Weight
• Fewer Screws –
novel vertical screwclamp technology
reduces the number
of screws required
for set up compared
to traditional largeformat systems,
dramatically reducing
assembly time
• No Top Tank
Assembly - A built-in
inner buffer chamber
within the PAGE
insert allows set up
to be completed
without inclusion of
a top tank or upper
buffer chamber
The VS20WAVE Maxi
Vertical Electrophoresis System
The new VS20WAVE Maxi System
is Cleaver Scientific’s latest product
innovation for large-format vertical gel
electrophoresis. Designed to perform
a variety of separations, including
first- and second-dimension SDSPAGE, native, preparative, gradient
and high-resolution nucleic acid
electrophoresis, plus capillary tube gel
IEF and electroblotting, the VS20WAVE
is one of the most versatile maxi vertical
systems available.
By introducing innovative, new vertical
screw-clamp technology within the
Plate
dimensions (w 20x20x0.4cm
x h x t)
2x20cm
fASTER SET UP:
4-5 hours
3-4 hours
30x18x27cm
2.5Kg
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Typical Applications
Large format separations of proteins
and nucleic acids
PAGE insert only four screws are now
necessary to secure as many 20x20cm
gels. This gives the VS20WAVE Maxi
the selective advantage of a much faster
set up speed compared to competitor
products whose traditional clamping
configurations require as many as 24
screws to secure just two glass plates. In
addition, the WAVE’s vertical screwclamp configuration distributes pressure
evenly along the height of the gel
rather than in the centre to eliminate
plate bowing and gel compression, but
still maintains a leak-proof seal during
casting; while the ergonomic wave-like
design of the PAGE insert aids both
handling and set up.
Whatever your requirements are the
WAVE can be made to meet them.
Regardless of whether it is running 2
or 4 gels, electroblotting, and IEF using
capillary tube gels or IPG strips, all of
these techniques may be performed
using the same omni-purpose unit
while retaining the benefits of large
format electrophoresis, such as
extended separation distances, greater
sample throughput and superior
resolution.
58
I vertical electrophoresis systems
vertical electrophoresis systems I 59
features:
ORDERING INFORMATION
VS20WAVECES • Economy of bench space and cost
1x WAVE Maxi Vertical Unit which includes: PAGE
insert, 2x4mm thick notched glass plates, 2x4mm
thick plain glass plates with 1mm thick bonded
spacers, 1x dummy plate, 2x combs (1mm thick 24
samples), 1x casting base, silicone mat, cooling coil;
• Enhanced reproducibility
• Simple to use casting
• Rapid set up cooling
plus: 1x WAVE Standard Electroblotting Module
which includes: WAVE Maxi Platinum Wire Blotting
Insert, 4x compression cassettes and 18x fibre pads;
and: 1x WAVEDCI Capillary Electrophoresis Module
which includes: WAVE Maxi Tube Gel Insert, 100x
capillary tubes,10x blanking ports; and 1x WAVE
IEF-KIT:1 set of plain glass plates with bonded
spacers, 0.6x20cm (w x h); and 2x 2-D combs with
one 3.5mm marker lane and one 18cm preparatory
well.
ORDERING INFORMATION
Modular Systems
CVS10CES Complete system for Mini Vertical Electrophoresis
comprising
1x omniPAGE Mini Vertical Unit which includes: 2x2mm thick
notched glass plates, 2x2mm thick plain glass plates with
Mini, Mini Wide and VS20Wave
Complete Modular Systems
1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm
thick 12 samples), 1x casting base, silicone mat, cooling pack.
The omniPAGE range of Modular Vertical Gel Systems allow
multiple electrophoresis techniques to be performed in the
same unit. These systems include all modules and accessories
required for Slab Gel Electrophoresis, 2-D Electrophoresis and
Electroblotting.
The central component is the omniPAGE Mini Vertical unit,
Mini Wide Vertical Unit or omniPAGE Maxi Vertical unit.
These include a rapid and intuitive casting system, enhanced
and easy to set up cooling system and have increased capacity
– can run up to four gels per run. In addition, the omniPAGE
Tube gel module is capable of resolving up to 10 first
dimension gels and the Electroblotting module has a four blot
capacity for the Mini system, and a three blot capacity for the
Mini Wide and Maxi. The package includes all the necessary
accessories for Slab Gel, First Dimension and Electroblotting.
Each of these techniques benefits from rapid set up cooling
packs which provide enhanced resolution even during high
intensity 2-D electrophoresis and Electroblotting.
blanking plugs, 2-D combs and spacers
VS20WAVECES-HI
plus: 1x Capillary Electrophoresis Module which includes:
internal running module for tube gels, capillary tubes,
Complete WAVE Maxi System 2-D electrophoresis
and high intensity blotting, comprising:
1x WAVE Maxi Vertical Unit which includes: PAGE
insert, 2x4mm thick notched glass plates, 2x4mm
thick plain glass plates with 1mm thick bonded
spacers, 1x dummy plate, 2x combs (1mm thick 24
samples), 1x casting base, silicone mat, cooling coil;
plus: 1x Electroblotting Module comprising: internal
electroblotting module, 4x compression cassettes for gel sizes
up to 10x10cm and 16x fibre pads.
VS10WCES
Complete WAVE Maxi System for 2-D
electrophoresis and blotting, comprising:
plus: 1x WAVE High Intensity Electroblotting
Module which includes: WAVE Maxi Plate Electrode
Blotting Insert, 1x compression cassette and 6x fibre
pads;
omplete Mini Wide (20x10cm) Vertical
C
Electrophoresis Modular System, comprising:
and: 1x WAVEDCI Capillary Electrophoresis Module
which includes: WAVE Maxi Tube Gel Insert, 100x
capillary tubes,10x blanking ports; and 1x WAVE
IEF-KIT:1 set of plain glass plates with bonded
spacers, 0.6x20cm (w x h); and 2x 2-D combs with
one 3.5mm marker lane and one 18cm preparatory
well.
1x omniPAGE Mini Wide Vertical Unit which includes:
2x4mm thick notched glass plates, 2x4mm thick plain
glass plates with
1mm thick bonded spacers, 1x dummy plate, 2x combs
(1mm thick, 24 samples), 1x casting base, silicone mat,
cooling pack.
plus: 1x Capillary Electrophoresis Module which includes:
internal running module for tube gels, capillary tubes,
blanking plugs, 2-D combs and spacers
The VS20WAVE complete electrophoresis system provides a
fully integrated solution using one universal gel tank and lid
for vertical PAGE, tube gel IEF and electroblotting.
plus: 1x Electroblotting Module
comprising: internal electroblotting module, 3x
compression cassettes for gel sizes
up to 20x10cm and 12x fibre pads.
TECHNICAL SPECIFICATION
TECHNICAL SPECIFICATION
IEF tube gel capacity
1-10
PAGE gel capacity
2 as standard; up to 4 maximum
Blotting Capacity
1-4
Buffer Volume 2-D Insert
640mL
Outer Tank Buffer Volume
5.3L
Tube Gel Dimensions
18x0.1cm (length x diameter)
Unit dimensions
(w x d x h)
Mini 19 x 13 x 15cm
Mini Wide 26 x 16 x 16cm
Maxi 26 x 16 x 28cm
Max. sample, Mini
Capacity
Slab 80 samples, 20 samples /gel
Tube - 10 tubes; Blot - 4 blots
Max. sample, Mini Wide
Capacity
Slab 192 samples, 48 samples /gel
Tube - 10 tubes; Blot - 4 blots
Plate Dimensions (w x h x t)
20x20x0.4cm
Max. sample, Maxi
Capacity
Slab 192 samples, 48 samples /gel
Tube - 10 tubes; Blot - 4 blots
2-D Gel Dimensions (w x h)
18.8x17.5cm
Buffer volume
Mini: Min 250ml; Max 1200ml
Mini Wide: Min 600ml; Max 2800ml
Maxi: Min 1200ml; Max 5600ml
Blotting cassettes dimensions
20x20cm
Typical Applications
Mini SDS PAGE protein separations, western blotting and
2-D tube gel electrophoresis.
The VS20WAVE
Complete
Electrophoresis
System
• Interchangeable modular inserts allow users to combine
vertical PAGE with capillary gel IEF to perform 2-D
electrophoresis, followed by western transfer for
enhanced sensitivity
• Scale-up your discovery projects using a large format,
high-resolution system
• Cooling supplied for temperature-sensitive separations
and samples
• High-intensity blotting system available for rapid transfers
Recommended power supply EV215 (Pg 89)
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Typical Applications
Maxi SDS PAGE protein separations, western blotting and
2-D tube gel electrophoresis.
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
60
I vertical electrophoresis systems
vertical electrophoresis systems I 61
ORDERING INFORMATION
CVS10C2DS Complete Mini 2-D System comprising:
1x omniPAGE Mini Vertical Unit which includes: 2x2mm
thick notched glass plates, 2x2mm thick plain glass plates
with
omniPAGE 2D
Systems
1mm thick bonded spacers, 1x dummy plate, 2x combs
(1mm thick 12 samples), 1x casting base, silicone mat,
cooling pack..
plus: 1x Capillary electrophoresis module which includes:
internal running module for tube gels, capillary tubes,
1x WAVE Maxi Vertical Unit which includes: PAGE
insert, 2x4mm thick notched glass plates, 2x4mm
thick plain glass plates with 1mm thick bonded
spacers, 1x dummy plate, 2x combs (1mm thick 24
samples), 1x casting base, silicone mat, cooling pack;
VS10WC2DS Complete Mini Wide 2-D System comprising:
1x omniPAGE Mini Wide Vertical Unit which includes:
2x4mm thick notched glass plates, 2x4mm thick plain
glass plates with
plus: 1x WAVEDCI Capillary Electrophoresis Module
which includes: WAVE Maxi Tube Gel Insert, 100x
capillary tubes,10x blanking ports; and 1x WAVE
IEF-KIT:1 set of plain glass plates with bonded
spacers, 0.6x20cm (w x h); and 2x 2-D combs with
one 3.5mm marker lane and one 18cm preparatory
well
1mm thick bonded spacers, 1x dummy plate, 2x combs
(1mm thick 24 samples), 1x casting base, silicone mat,
cooling pack.
plus: 1x Capillary electrophoresis module which includes:
internal running module for tube gels, capillary tubes,
blanking plugs, 2-D combs and spacers.
ORDERING INFORMATION
VS20WAVEDCI
WAVE Maxi Tube Gel insert – includes glass tubes
and blanking ports, plus WAVEIEF-KIT
VS20WAVE-IEFKIT
IEF Conversion Kit for 18cm IPG strips and tube
gels,
includes: 1 set of plain glass plates with bonded
spacers, 0.6x20cm (w x h); and 2x 2-D combs with
one 3.5mm marker lane and one 18cm preparatory
well
Tube Gel Units, Inserts and Accessories
VS10DC
omniPAGE Mini Tube Gel Unit, 10x10cm with tank and lid,
glass capillary tubes, blanking ports & cooling pack
VS10DCI
omniPAGE Mini Tube Gel Insert - includes glass tubes and
blanking ports
VS10WDC
omniPAGE Mini Wide Tube Gel Unit, 20x10cm with tank
and lid, glass capillary tubes, blanking ports & cooling pack
VS10WDCI
• Rapid set-up electrofocusing
omniPAGE Mini Wide Tube Gel Insert - includes glass tubes
and blanking ports
MCT10
Mini Capillary Tubes, pk/100
• 10 tube capacity
VS20DCI
omniPAGE Maxi Tube Gel Insert - includes glass tubes and
blanking ports
• Extended accessory range
MCT20
Maxi Capillary Tubes, pk/100
• Enhanced cooling features
MCBP
Mini and Maxi Capillary Blanking ports
MCT101.5
Mini Capillary Tubes, 1.5mm, pk/100
MCT201.5
Maxi Capillary Tubes, 1.5mm, pk/100
features:
VS20WAVEC2DS Complete WAVE Maxi 2-D System comprising:
blanking plugs, 2-D combs and spacers.
Mini, Mini Wide and Wave Complete
2-D Systems
The omniPAGE 2-D Systems include both modules
required for Slab Gel and First Dimension Electrophoresis
and accessories, to provide a complete Mini, Mini Wide
or Wave 2-D system. The Tube Gel Module includes a
rapid release gasket for easy tube extraction. Focusing
can be accomplished in as little as three hours in the Mini
Unit. Disposable capillary tubes are included for added
convenience, plus 2-D combs and spacers which are colour
coded according to thickness for easy identification.
ORDERING INFORMATION
Technical Specifications
IEF tube gel capacity
1-10
Number of second-dimension PAGE
2 as standard; up to 4 maximum
gels
Tube Gel Dimensions
18x0.1cm (length x diameter)
Plate Dimensions (w x h x t)
20x20x0.4cm
2-D Gel Dimensions (w x h )
18.8x17.5cm
Running conditions for IEF with
cooling
Up to 20 hours; 800V maximum
Recommended power supply for
IEF
EV215 (Pg 89)
Typical Applications
Full evaluation of protein samples.
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
The VS20WAVE 2D
Tube Gel System
The VS20WAVE 2-D tube gel system provides all necessary
components for 2-D electrophoresis using capillary
ampholyte tube gels.
• Interchangeable modular inserts run capillary tube gels
for first-dimension IEF and vertical slab gels for seconddimension SDS-PAGE using the same universal tank and
lid
• Detachable cooling coil, which connects to the laboratory
water supply or a recirculating chiller, enables application
of high voltages to achieve precise, high-resolution
focusing for pI determination
• Focus up to 10 first-dimension IEF tube gels within a
single run using the dedicated 2-D insert, or use included
blanking ports to run fewer gels if preferred
• Run up to four 20x20cm second-dimension slab gels
using specially adapted notched glass plates with bonded
2-D spacers to accommodate 18cm tube gels
• System may also be used for 18cm IPG strips resolved in
the first-dimension on the CSL-IEF flat-bed IEF system
(Pg 63)
• Two 24-sample 1mm combs included for routine SDSPAGE techniques
62
I vertical electrophoresis systems
ORDERING INFORMATION
CSL-IEF
Flatbed IEF system for IPG strips and gels, with
focusing and rehydration trays
CSL-CHILLER
Chiller for electrophoresis systems
CSL-IEF-KIT
1-D Combination Package, includes CSL-IEF,
CSL-CHILLER and EV233
CSL-IEFPOS
vertical electrophoresis systems I 63
CSL-IEF
TYPICAL RUNNING CONDITIONS
7cm IPG Strip
IEF Step
1
2
3
4
5
6
Voltage (V)
150
300
600
1500
3000
300
Replacement positive electrode
Time (h)
0.5
0.5
0.5
0.5
2.5
<20
CSL-IEFNEG
Replacement negative electrode
Volt-hours
75
150
300
750
7500
-
CSL-IEFPLT
Replacement glass platform
EV233
Consort 3000V, 300mA, 300W power supply
CSL-IEFFRME
Replacement electrode frame
CSL-RHDTRAY
Rehydration Tray and lid
CSL-FOCUSTRAY
Focusing Tray
CSL-FTELECPOS
Focusing Tray Adjustable Electrode Positive
features:
CSL-FTELECNEG
Focusing Tray Adjustable Electrode Negative
• For IPG strips and
IEF gels
18cm IPG Strip
IEF Step
1
2
3
4
5
Voltage (V)
300
600
1500
3000
300
Time (h)
0.5
1
1
12
<20
Volt-hours
150
600
1500
36000
-
• Large cooling
platform area
TRAY SPECIFICATIONS
IPG Strip Length
7cm
11cm*
18cm
• ‘Pick-and-Place’
adjustable
electrodes
24cm
• Focusing tray for a
maximum twelve
IPG strips
FOCUSING TRAY
Electrode Distance
6.5cm
10.2cm
17.1cm
22.7cm
Maximum Strip Length
Accommodated
25.3cm
25.3cm
25.3cm
25.3cm
Cleaver Scientific IPG
Strip Length
7cm
n/a*
18cm
24cm
CSL-IEF TECHNICAL SPECIFICATIONS
Max. commercial strip length
accommodated
24cm
Max. gel dimensions on cooling plate
26x26cm
Unit dimensions (w x d x h)
55x35x10cm
Recommended Volume
for Strip Rehydration
24cm
3.5ml
24cm
6ml
*11cm strips available from other suppliers
24cm
Focusing tray strip capacity
12x 18 and 24cm strips;
24x 7 and 11cm strips
8.0ml
Operating temperature
4-25°C
Regulatory certification
CE, EN61010
24cm
12.0ml
Features include:
• A high-capacity focusing tray that
accommodates up to twelve IPG
strips
• A cooling plate, manufactured
from a special grade ceramic in
a large 26x26cm surface area,
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Now equipped with rehydration and
focusing trays, the redesigned CSL-IEF
has been optimised to perform first-dimension isoelectric focusing (IEF) with
IPG (immobilised pH gradient) strips
quickly, easily and reproducibly.
An ideal entry-level system for both
inexperienced and occasional IEF users,
the CSL-IEF is also versatile enough to
meet the needs of laboratories with
increased throughput requirements.
• Adjustable ‘pick-and-place’
electrodes clip conveniently
anywhere within the focusing tray to
resolve IPG strips 7-24cm in length;
colour-coded to prevent polarity
reversal
REHYDRATION TRAY
Maximum Strip Length
Accommodated
Isoelectric Focusing
1st dimension of 2-D electrophoresis
separation of proteins according to
their PI
facilitates effective heat dissipation
and homogenous thermal control,
particularly during high voltage
IEF techniques
• An optional, but recommended,
Cleaver Scientific recirculating chiller
(Pg 130) connects quickly and easily
to the cooling plate via snap-lock
connectors to maintain optimal
operating temperatures for IPG strips
(20°C) and precast gels (4°C)
• Rehydration tray allows convenient
transfer of IPG strips to the focusing
tray without time-consuming
removal of residual rehydration
buffer; also enables focusing tray to
remain permanently in use for IEF to
maximise throughput, and provides
useful storage at -20°C for focused
strips before second-dimension runs
• Electrode frame clips directly on
to the cooling plate and includes
adjustable electrodes to run
horizontal precast IEF and PAGE gels
64
I vertical electrophoresis systems
vertical electrophoresis systems I 65
Ordering Information
VS30D omniPAGE Maxi Plus 30 x 22cm Dual with Glass Plates with
bonded 1.5mm spacers, 2 x 28 sample combs, 2 x 2-D combs, cooling
pack, dummy plate
VS30DSYS
VS30D with Casting Base
VS30BI
omniPAGE VS30 Blot Maxi Insert - includes 3 cassettes and 6 fibre
pads.
VS30DCAST
30 x 22cm Dual Casting Base
VS30DCASTM
Replacement Silicone Mat for 30 x 22cm Casting Base
VS30DIRM
Inner Running Module
VS30ICB
Maxi Cooling Pack
VS30-x -LG
Loading guides for omniPAGE maxi combs, x = comb well number
VS30NG
30 x 22cm Notched Glass Plates 4mm thick (pk/2)
VS30PG
30 x 22cm Plain Glass Plates 4mm thick (pk/2)
VS30NGS0.75
30 x 22cm Notched Glass Plates with 0.75mm Bonded Spacers (pk/2)
VS30PGS0.75
30 x 22cm Plain Glass Plates with 0.75mm Bonded Spacers (pk/2)
VS30NGS1
30 x 22cm Notched Glass Plates with 1mm Bonded Spacers (pk/2)
VS30PGS1
30 x 22cm Plain Glass Plates with 1mm Bonded Spacers (pk/2)
VS30PGS1.5
30 x 22cm Plain Glass Plates with 1.5mm Bonded Spacers (pk/2)
VS30PGS2
30 x 22cm Plain Glass Plates with 2mm Bonded Spacers (pk/2)
VS30DP
Dummy Plate, 30 x 22cm
VS30S0.75
22cm Spacers - 0.75mm (pk/2)
Second stage 2-D
VS30S1
22cm Spacers - 1mm thick (pk/2)
VS30S1.5
22cm Spacers - 1.5mm thick (pk/2)
VS30S2
22cm Spacers - 2mm thick (pk/2)
The omniPAGE VS30 maxi-plus large format vertical has
been designed as a convenient unit for second-dimension
PAGE following first-dimension isoelectric focusing using the
CSL-IEF.
RPW-0.2100
Replacement Platinum Wire - 0.2mm, 100cm
An active gel width of 26cm easily accommodates IPG strips
up to 24cm in length, the longest available commercially,
while an extended gel height of 22cm maximises separation
distance and resolution of proteins similar in size or
isoelectric point (pI). In combination with the CSL-IEF, the
VS30DSYS provides a complete 2-D electrophoresis system
which utilises the advanced features of the omniPAGE range
to produce a unit that is both easy to use and consistent in
generating reproducible results.
Rapid set-up cooling retains resolution in extended
separations and also conserves buffer volume without
affecting run quality. A maximum of four 1-mm-thick
gels may be resolved per run, using notched glass plates
with 1mm bonded spacers; and a comprehensive range of
accessories facilitate easy interchange between 2-D and
standard vertical electrophoresis techniques. Different types
of 2-D comb offer a wide degree of versatility in sample
selection and gel set-up.
Typical Applications
Larger gel size. Running IPG strips
Gradient Mixers
features:
ACCESSORIES
• Ideal for second-dimension electrophoresis
• Accepts IPG strips 24cm in length, the longest
Code
Description
VS30-1-1
Comb 1 Prep, 1 Marker, 1mm thick
2250µl
VS30-2-1
Comb 2 sample, 1mm thick
1125µl
VS30-4-1
Comb 4 sample, 1mm thick
550µl
VS30-28MC-1
Comb 28 sample, 1mm thick MC compatible
80µl
VS30-56MC-1
Comb 56 sample, 1mm thick MC compatible
40µl
VS30-75-1
Comb 75 sample, 1mm thick MC compatible
25µl
VS30-1-1.5
Comb 1 Prep, 1 Marker, 1.5mm thick
3375µl
VS30-2-1.5
Comb 2 sample, 1.5mm thick
1680µl
VS30-4-1.5
Comb 4 sample, 1.5mm thick
VS30-28MC-1.5
available commercially
• Rapid set-up cool packs enhance resolution,
particularly during extended runs
Technical Specifications
Unit dimensions (w x h x d)
36x33x18cm
Plate dimensions (w x h)
30x22cm
Gel dimensions (w x h)
28x20cm
Max. sample capacity
300 samples per run; 75 per gel
Buffer volume
1800-8400ml
Comb options
Sample Volume
per well
Ideal for Caesium, Sucrose and Gel gradients these Gradient Mixers comprise two chambers – a reservoir and a
mixing chamber with an interconnecting valve. A second
valve regulates the output flow from the mixing chamber.
All mixers have a flat base which allows them to be placed
on a magnetic stirrer. A magnetic stirring bar can be placed
directly in the mixing chamber to ensure a constant gradient.
The support rod allows the mixer to be fixed to a retort
stand for extra stability.
Ordering Information
CSL-GM15
15ml Gradient Mixer
825µl
CSL-GM25
25ml Gradient Mixer
Comb 28 sample, 1.5mm thick MC compatible
120µl
CSL-GM50
50ml Gradient Mixer
No. of teeth
1, 2, 4, 28MC, 56MC, 75
VS30-56MC-1.5
Comb 56 sample, 1.5mm thick MC compatible
60µl
CSL-GM100
100ml Gradient Mixer
Thicknesses
0.25, 0.35, 0.5, 1, 1.5, 2
VS30-75-1.5
Comb 75 sample, 1.5mm thick MC compatible
37µl
CSL-GM500
500ml Gradient Mixer
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Typical Applications
Making gel, caesium and sucrose gradients
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
66
I vertical electrophoresis systems
vertical electrophoresis systems I 67
Vertical Electrophoresis Packages
Whether you require a package to match the market leader or one to address any budget or vertical electrophoresis
technique, Cleaver Scientific now offers the ultimate range of Combination Packages that will meet your needs.
ORDERING INFORMATION
CVS10TETRAD
CVS10TETRAD1CBS
Run 4 gels in the tank while
casting another 4 externally
1OmniPAGE TETRAD Mini-Protein
Electrophoresis Package for 1mm self-cast
gels, includes:
CVS10DSYS-CU
VS10-12-1
VS10NGS1
With interchangeable
4-blot module
OmniPAGE TETRAD with interchangeable
4-blot module, includes:
10x10cm mini-vertical unit with
casting base and external casting
stand, plus 2x 1mm 12-sample combs, CVS10TETRAD1
2x plain glass plates with 1mm spacer
and 2x notched plates (Pg 51)
2 additional 1mm 12-sample combs,
1 pack of 2x plain glass plates with
VS10BI
1mm spacer
1 pack of 2x notched glass plates
2 packs of 2x notched glass plates
with 1mm spacer
OmniPAGE TETRAD Mini-Protein
Electrophoresis Package for 1mm
self-cast gels
4-blot insert with 4 blotting cassettes
and 8 fibre pads (Pg 74)
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CVS10TETRAD1-CS300
With standard midi power
supply option
CVS10CBS-CS300
Mini vertical, power supply and
blotting package
ORDERING INFORMATION
VS10WDSYS-CS300
Wide mini vertical self cast
package
OmniPAGE TETRAD with standard Midi
power supply option, includes:
CVS10TETRAD1
OmniPAGE TETRAD Mini-Protein
Electrophoresis Package for 1mm
self-cast gels
300V, 700mA, 150W midi power
supply (Pg 87)
CS-300V
For 0.75, 1.5 and 2mm versions use CVS10TETRAD0.75For 0.75, 1.5 and 2mm versions use CVS10TETRAD0.75CBS,
CS300, CVS10TETRAD1.5-CS300 and CVS10TETRAD2CVS10TETRAD1.5CBS and CVS10TETRAD2CBS respectively
CS300 respectively
VS10WCBS-CS300
Wide mini vertical power supply
and blotting package
Self-cast gel mini-wide vertical
electrophoresis package includes
CVS10CBS
omniPAGE Mini-Protein system with
VS10WDSYS
casting base and blotting insert (Pg 74)
omniPAGE Mini Wide Protein system
with casting base (Pg 55)
VS10WCBS
omniPAGE Mini Wide Protein system
with casting base and blotting insert
CS-300V
300V, 700mA, 150W midi power
supply (Pg 87)
300V, 700mA, 150W midi power
supply (Pg 87)
CS-300V
300V, 700mA, 150W midi power
supply (Pg 87)
For packages with high current power supply, use codes
CVS10PRE-CS3AMP, CVS10DSYS-CS3AMP, CVS10CBSCS3AMP; combs and glass plates with bonded spacers may
be changed at order
CS-300V
For 0.75, 1.5 and 2mm versions use
CVS10TETRAD0.75CBS-CS300, CVS10TETRAD1.5CBSCS300 and CVS10TETRAD2CBS-CS300 respectively
For packages with high current power supply, use codes
VS10WDSYS-CS3AMP, VS10WCBS-CS3AMP; combs and
glass plates with bonded spacers may be changed at order
For 0.75, 1.5 and 2mm versions use CVS10TETRAD0.75,
CVS10TETRAD1.5 and CVS10TETRAD2 respectively
WAVETETRAD1
CVS10TETRAD1CBS-CS300
with standard midi power supply
option and interchangeable
4-blot module
OmniPAGE TETRAD with Midi power
supply and interchangeable 4-blot
module, includes:
CVS10PRE-CS300
Standard 4-gel maxi vertical
package
Standard 4-gel maxi vertical
package
Single Workstation 4-gel Maxi
Vertical package, complete with
general purpose or specialist
power
4-gel electrophoresis package: run 4 gels
in the tank and cast another 4 externally
includes:
With interchangeable 4-blot running
module includes:
4-gel electrophoresis package with general
purpose Maxi programmable
power supply includes:
CVS10DSYS-CS300
Pre-cast gel mini-vertical
package
Self cast and pre cast mini
vertical package
Pre-cast gel mini-vertical
electrophoresis package includes:
Pre-cast and hand cast gel mini-vertical
electrophoresis package includes:
CVS10TETRAD1CBS
OmniPAGE TETRAD Mini-Protein
Electrophoresis Package for
1mm self-cast gels with blotting
module
CVS10PRE
omniPAGE Mini-Protein system
(Pg 51)
CVS10DSYS
omniPAGE Mini-Protein system with
casting base (Pg 51)
CS-300V
300V, 700mA, 150W midi power
supply (Pg 87)
CS-300V
300V, 700mA, 150W midi power
supply (Pg 87)
CS-300V
300V, 700mA, 150W midi power
supply (Pg 87)
For 0.75, 1.5 and 2mm versions use
CVS10TETRAD0.75CBS-CS300, CVS10TETRAD1.5CBSCS300 and CVS10TETRAD2CBS-CS300 respectively
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
WAVETETRAD1-CS500
WAVETETRAD1CBS
VS20WAVE Maxi, 20 x 20cm Dual,
2 sets of plain glass plates with
1mm thick bonded spacers, 2 set of
VS20WAVESYS-CU notched glass plates 2 x 24 sample,
1mm thick combs, cooling coil,
dummy plate; includes caster and
external casting upstand
VS20NGS1
2 packs of 2x notched glass plates
with 1mm bonded spacers
WAVETETRAD1
4-gel electrophoresis package
WAVETETRAD1
4-gel electrophoresis package
VS20BI
1x WAVE Standard Electroblotting
Module for 4 cassettes (Pg 78)
CS-500V
500V, 800mA, 300W maxi
programmable power supply (Pg 88)
VS20-24-1
2 additional 1mm 24-sample combs
Typical
Applications
xx
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
68
I vertical electrophoresis systems
vertical electrophoresis systems I 69
ORDERING INFORMATION
CSQ20
Large Format Vertical, 20cm wide, glass plates,
0.35mm spacers, 48 sample comb
CSQ20-NG
Glass plates, pk/2 Notched
CSQ20-PG
Glass plates, pk/2 Plain
CSQ20-S0.25
Spacer set 0.25mm
CSQ33
Large Format Vertical, 33cm wide, glass plates,
0.35mm spacers, 48 sample comb
COMB SPECIFICATIONS
Sample
Volume
per
well
Code
Description
CSQ20-0.25-24
Comb 24 sample, 0.25mm thick, Sharks
tooth
7µl
CSQ20-0.25-48
Comb 48 sample, 0.25mm thick, Sharks
tooth
3µl
CSQ33-0.25-48
Comb 48 sample, 0.25mm thick, Sharks
tooth
7µl
CSQ33-0.25-96
Comb 96 sample, 0.25mm thick, Sharks
tooth
3µl
CSQ20-0.35-24
Comb 24 sample, 0.35mm thick, Sharks
tooth
9µl
CSQ33-NG
Glass plates, pk/2 Notched
CSQ33-PG
Glass plates, pk/2 Plain
CSQ33-S0.25
Spacer set 0.25mm
CSQ20-0.35-48
Comb 48 sample, 0.35mm thick, Sharks
tooth
5µl
CSL-FHS*
Fan heater sensor kit for large format vertical units
CSQ20 and CSQ33 220V
CSQ33-0.35-48
Comb 48 sample, 0.35mm thick, Sharks
tooth
9µl
CSQ20-S0.35
Spacer set 0.35mm
CSQ33-0.35-96
Comb 96 sample, 0.35mm thick, Sharks
tooth
5µl
CSQ20-S1
Spacer set 1mm
CSQ20-1-24
Comb 24 sample, 1mm thick, Square tooth
40µl
CSQ20-S1.5
Spacer set 1.5mm
CSQ20-1-48
Comb 48 sample, 1mm thick, Square tooth
20µl
CSQ33-1-48
Comb 48 sample, 1mm thick, Square tooth
35µl
CSQ33-S0.35
Spacer set 0.35mm
CSQ33-1-80
Comb 80 sample, 1mm thick, Square tooth
20µl
CSQ33-S1
Spacer set 1mm
CSQ20-1.5-24
Spacer set 1.5mm
Comb 24 sample, 1.5mm thick, Square
tooth
60µl
CSQ33-S1.5
CSQ-FC
Flexi caster for csq20 & csq33
CSQ20-1.5-48
Comb 48 sample, 1.5mm thick, Square
tooth
30µl
CSQ33-1.5-48
Comb 48 sample, 1.5mm thick, Square
tooth
50µl
CSQ33-1.5-80
Comb 80 sample, 1.5mm thick, Square
tooth
30µl
CSL-MGR
Mini Glass Plate Rack for 20x 2mm Plates
CSL-LGR
Large Glass Plate Rack for 10x 5mm Plates
features:
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• Run up to 96
samples
• Enhanced gel heat
homogenisation
• Variable low or high
buffer volumes
• 20 x 50cm or 33 x
45cm formats
Large Format Vertical
Plate Racks
Ideal for a variety of large format
vertical gel applications, these units
offer advanced features for enhancing
gel resolution and ease of use, essential
when handling gels of this size. Each
unit contains ultra soft silicone seals
for easy plate sealing and trouble free
runs, even over extended run times.
These sturdy racks are designed for
safe drying and storage of glass plates.
The small rack can hold up to 20x 2mm
thick plates while the larger rack can
accommodate up to 10x 5mm thick
glass plates.
Resolution is enhanced by using an
aluminium heat sink plate, essential
for even sample migration. Added
convenience is provided by a
removable lower buffer tank and upper
buffer drainage tap.
*100V add $ to stock code when ordering
technical SPECIFICATIONs
Plate dimensions
(w x l)
CSQ20 20 x 50cm
CSQ33 33 x 45cm
Max sample
capacity
CSQ20 48 samples
CSQ33 96 samples
Buffer Volume
CSQ20 Min 500ml, Max 1000ml
CSQ33 Min 800ml, Max 2000ml
Combs available:
No. of teeth
Thicknesses
24, 48, 80, 96
0.25, 0.35, 1, 1.5mm
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
DNA Sequencing, 2-D analysis, Micro-satellite analysis, DNA fingerprinting, Gel
shift assays, Single-Strand Conformation
Polymorphism (SSCP), Heteroduplex and
Oligonucleotide analysis
Special buffer chambers allow either
low buffer volumes to be used for
economy or high buffer volumes to be
used for extended runs.
A wide range of interchangeable
comb and spacer options allows a
large number of techniques to be
easily accomplished including; DNA
Sequencing, 2-D analysis, Microsatellite analysis, DNA fingerprinting,
Gel shift assays, Single-Strand
Conformation Polymorphism (SSCP),
Heteroduplex and Oligonucleotide
analysis.
70
I vertical electrophoresis systems
vertical electrophoresis systems I 71
TECHNICAL SPECIFICATION
WAVE ELECTROPHORESIS INSERT AND TANK
Max. Number of Gels
2 per run
Plate Dimensions (WxH)
20x20cm
Active Gel Dimensions (WxH)
16x17.5cm
Spacer Thicknesses
0.75, 1, 1.5 and 2mm
Max. Sample Capacity
96 samples; 48 per gel
Standard Combs
2x 1mm 24-sample
Available Combs
1, 5, 10, 18MC, 24, 36MC, 48; as per
VS20WAVE unit (pg 34 & 30 )
Max. Buffer Volume
8.5L
Unit Dimensions (w x d x h)
40.5 x 17 x 44cm
Weight
8kg
RECOMMENDED POWER SUPPLY
Voltage
500V
Current
800mA/500mA
Power
300W/150W
TEMPERATURE CONTROL UNIT
VS20WAVE-DGGE - Applications
DGGE
Description
i. Determines the denaturing
conditions required to identify
unknown mutations
ii. Works on the principle
ii. New VS20WAVE
that increasing denaturant
electrophoresis insert and cam
caster for leak free casting
concentrations melt DNA in a
domain-specific manner, and the
mutation or polymorphism of
interest is in the DNA domain with
the lowest Tm
iii. Temperature control
iii. Requires parallel DGGE – a
technique where DNA samples are unit provides consistent run
resolved at uniform temperature in temperatures between 45-70°C
gels containing a formamide and
urea denaturant gradient parallel
to the direction of electrophoresis
iv. Results in partial melting of
DNA to produce a branched
molecule identified by its reduced
mobility within the gel
Temperature Control
PID
Operating Temperature Range
Ambient-100°C
Working Temperature Range
(DGGE)
45-70°C
CDGE
Buffer Recirculation Mechanism
Stirring
Description
Temperature Uniformity/Stability
at 37°C
±0.05/0.02°C
Setting/Display Resolution
0.1°C
Safety
Fluid-level float switch; isolated;
IEC 1010 / CE
Stored Temperature Values
4
Heater Power at 230V/110VAC
1.4/1.3kW
Electrical Power at 230V/100VAC 1.5/1.4kW (50-60Hz)
GRADIENT MIXER
Total Volume
100ml
Volume of Reservoir & Mixing
Chambers
50ml
Internal Diameter of Outlet Port
2mm
ORDERING INFORMATION
VS20WAVE-DGGE
Complete Denaturing Gradient Gel Electrophoresis System,
20x20cm; includes: temperature control unit, cam casting
base, glass plates with 1mm bonded spacers, 2x 24-sample
combs and gradient mixer – 240 VAC version
VS20WAVE-DGGE$
VS20WAVE-DGGE – 110VAC version
VS20WAVE-DGGETC
VS20WAVE-DGGE Temperature Control Unit – 240VAC
version
VS20WAVE-DGGETC$
VS20WAVE-DGGETC – 110VAC version
GM100
Gradient Mixer, 100ml (pg 29)
RECOMMENDED ACCESSORIES
CSL-STIR
CSL Magnetic Stirrer, 19x19cm (pg 76)
MU-D01
Single Peristaltic Pump (pg 76)
MU-S16
Silicon tube I.D. 1/8”, 25 ft (for peristaltic pump, pg 76)
CS-500V
omniPAC Power Supply, 500V, 800mA, 300W (pg 61)
SOFTWARE OPTIONS
Phoretix 1D
1D image analysis with band pattern matching (pg 66)
Phoretix 1D Pro
1D image analysis with band pattern matching between
different gels (pg 66)
Benefits
i. GM100 gradient mixer and
optional MU-D01 peristaltic pump
simplify casting of denaturing
gradient gels
iv. High resolution 20x20cm
format
features:
• Maximum 96-sample
throughput compatible with
microplates and thermal
cycler blocks
• Four-screw vertical clamping
technology accelerates set up
• Large format – 20x20cm glass
plates for improved resolution
• 100ml gradient mixer,
with valve-controlled
50ml reservoir and mixing
chambers, makes two 1mm
parallel denaturing gradient
gels
• Microprocessor-controlled
Benefits
i. Rapid screening method for
multiple samples containing an
identified mutation
i. Uses constant denaturant
gels cast with new VS20WAVE
electrophoresis insert and cam
caster for leak free casting
ii. Requires DGGE beforehand
to establish optimal denaturing
conditions to identify a specific
mutation
ii. Temperature control unit
provides constant run temperature
during electrophoresis
iii. No denaturant gradient
required as multiple samples are
screened in a constant denaturant
gel
iii. Maximum 96-sample
throughput (48 samples per gel)
iv. Increases throughput and
alleviates bottlenecks
temperature control unit
accurate to ±0.02°C
Denaturing Gradient Gel Electrophoresis (DGGE) is an important technique
used in the search for mutations and
DNA polymorphisms critical in genetic
disorders and cancers, and to understand genetic diversity among species.
Supplied with the VS20WAVE-DGGE
are:
HA
Benefits
The GM100 Gradient Mixer
i. Used when it is difficult to detect
a homoduplex mutation by DGGE
i. New VS20WAVE
electrophoresis insert and cam
caster for leak free casting
ii. Requires denaturation and reannealing of wild-type and mutant
DNA mixed together, usually
within a PCR reaction
ii. Gradient mixer simplifies DGGE
option
• Forms efficient gradients by mixing
and delivering high- and low-density
denaturant solutions.
iii. Resultant heteroduplexes
are less stable and melt at a
lower denaturant concentration
than wild-type and mutant
homoduplex molecules, allowing
them to be identified by reduced
mobility within the gel
iii. Optional temperature control
for reproducibility
iv. Requires parallel DGGE, or
may be performed overnight in a
TBE gel made from special highresolution acrylamide
iv. High resolution 20x20cm
format
Description
• The flat-base design and support
handle of the GM100 allows it to be
secured to a retort stand and mounted
on a magnetic stirring plate (e.g. CSLSTIR, Pg 124).
• The mixing chamber accommodates
a magnetic stirrer to form a linear
gradient.
• Optional MUD01 peristaltic pump
recommended.
DGGE PACKAGE
VS20WAVE-DGGEKIT
VS20WAVE Package Deal; includes: VS20WAVE-DGGE,
CSL-STIR, MU-D01, MU-S16, CS-500V – 240 VAC version
VS20WAVE-DGGEKIT$
VS20WAVE-DGGEKIT – 110 VAC version
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Typical Applications
Detecting mutations in genetics and
cancer studies.
Denaturing Gradient
Gel Electrophoresis
The VS20WAVEDIRM PAGE Insert and
Casting Base
• Employs innovative vertical screw
clamp technology to assemble two
vertical gels.
• Four-screw set up makes casting
assembly faster.
• A built-in inner buffer chamber
eliminates the need for heavy top
tanks or buffer chambers.
• Its dual purpose PAGE insert makes
plate transfer unnecessary, and is
used with a cam casting base to
guarantee efficient leak free casting
• Utilises all of the combs, glass
plates and accessories of existing
VS20WAVE units, providing full
flexibility.
• Two 1mm 28-sample combs are
supplied as standard while optional
48-sample combs allow screening
to take place directly from 96-well
thermal cycler blocks after PCR®
amplification.
The VS20WAVE-DGGETC Temperature
Controller
• Combines buffer recirculation
with a heat sensor and 1.4kW
heating element to facilitate precise
temperature control to within
±0.02°C.
• Allows the gel temperature to be set
to the melting temperature (Tm) of
the amplified DNA polymorphism or
mutation of interest.
• Features include: 4-digit 16mm
LED panel; precise tuning to within
0.1°C resolution; an operating set
point, plus three adjustable pre-set
temperature values; and stirred buffer
circulation for temperature stability
and uniformity.
BLOTTING SYSTEM SELECTION GUIDE
Use the selection guide below to identify the Cleaver Scientific blotting system best suited to your electrophoresis application.
Gel Capacity
Transfer Parameters
Blotting
Area
(WxL)
Number
of blotting
Cassettes
Required
Buffer
Volume
Electrode
Distance
omniPAGE Gels (WxH) / blotting cassette
Typical
Transfer
Time
Type of
VS20
VS10W
VS30
Blotting
CVS10
WAVE
Mini Wide
Maxi Plus w = western
Cooling
Mini
8x8.5cm 16x8.5cm 16x17.5cm 26x20cm n = northern
s = southern
10x10cm
4 with
wire / 2
with plate
electrodes
1.2L
8cm wire
/ 2cm
plate
1-2h / <1h Cool
pack
1
–
–
–
w
VS10WCBS
20x10cm
3
2.8L
6cm
1-2h
2
1
–
–
w
VS20CBS
20x20cm
4 with
wire / 1
with plate
electrodes
6.4L
8cm wire
/ 4cm
plate
5-20h /
1-5h
SB10
10x10cm
4 with
wire / 2
with plate
electrodes
1.2L
8cm
1-2h / <1h Cool
pack
1
–
–
–
w
SB10W
20x10cm
3
2.8L
6cm
1-2h
2
1
–
–
w
SB20
20x20cm
4 with
wire / 1
with plate
electrodes
6.4L
8cm wire
/ 4cm
plate
5-20h /
1-5h
Coil
4
2
1
–
w
EBM10
10x10cm
5
1.5L
12cm
1-2h
Cool
pack
1
–
–
–
w
EBM20
20x20cm
5
1.6L
15cm
5-20h
4
2
1
–
w
SD10
10x10cm
–
5ml
15-30 min
–
1
–
–
–
w,s,n
SD20
20x10cm
–
20ml
As per
sandwich
thickness
15-30 min
–
4
2
1
–
w,s,n
Tank Transfer
CVS10CBS
Coil
w
SD33
33x45cm
–
75ml
15-30 min
–
20
10
5
2
w,s,n
SD50
20x50cm
–
50ml
15-30 min
–
13
6
2
1
w,s,n
MICROFILTRATION (DOT AND SLOT BLOTTING) SELECTION GUIDE
Cleaver Scientific Microfiltration Manifolds do not require the resolving power of electrophoresis before transfer, with nucleic acid,
protein and antibody samples being simply drawn onto a membrane under the power of vacuum.
Blot Parameters
Membrane
Size Required
Configuration
Size of Well in
Loading Template
6mm diameter,
12mm deep
CSL-D48
12.1x4.4cm
3x16-well Dot
Array
CSL-D96
11x7.4cm
8x12-well Dot
Array
CSL-S24
12.1x4.4cm
2x12-well Slot
Array
CSL-S48
12.1x4.4cm
3x16-well Slot
Array
6x0.5mm Slot,
12mm deep
Microtitre Plate Compatibility / Capacity
Vacuum Required
600mg Hg 0.8
BAR with cold
trap
24-well
48-well
96-well
Cleaver Scientific offers
four types of system:
• Modular
Electroblotters –
combine PAGE and transfer
techniques within the same
tank (see Pg 74-75 ).
• Tank transfer systems
– available with either plate
or wire electrodes, support
efficient, quantitative
transfers over a wide
molecular weight range.
Plate electrode systems are
faster through greater field
strength; wire electrodes
are more economical,
consuming less current and
generating less heat (see Pg.
77 ).
• Semi-dry transfer
systems – perfect for rapid,
high-intensity transfers of
mid-range proteins, 10100kD in size (see Pg. 80 ).
• Microfiltration (dot
and slot blotting)
– does not require
electrophoresis and is used
to determine the working
conditions for a new blotting
assay, antibody titres and
antibody-antigen specificity.
Also suitable for nucleic
acids (see Pg. 81 ).
Type of
Blotting
w = western
n = northern
s = southern
Yes / 2x
plates
Yes / 1x
plate
–
w,s,n
Yes / 4x
plates
Yes / 2x
plates
Yes / 1x
plate
w,s,n
Yes / 1x
plate
–
–
w,s,n
Yes / 2x
plates
Yes / 1x
plate
–
w,s,n
BLOTTING
SYSTEMS
Blot Transfer
Systems Overview:
Blotting, a technique that entails immobilisation of
proteins or nucleic acids on a solid membrane support
and then detection using a specific antibody or probe of
complementary nucleic acid sequence, significantly increases
the potential for identification and characterisation of
proteins and nucleic acids. Upon transfer to a membrane
support proteins and nucleic acids become far more
accessible to detection by antibodies and probes than they
would otherwise be within a gel. Separating out DNA,
RNA or Protein samples of different sizes by electrophoresis
followed by blotting is an excellent way to identify specific
molecules within a mixed population of nucleic or protein
molecules, and the two techniques are often used in tandem.
Selection Guide
72
omniPAGE Electroblotting
74-76
Tank Sub Electroblotters
77
omniPAGE Sub Blot High Intensity Systems
and Electroblotters
78-79
Semi Dry Blotters
80
Dot and Slot Blotters
81
RELATED PRODUCTS
POWER SUPPLIES PAGES 82-89
VACUUBRAND GEL
PUMP PAGE 131
BLOTTING
MEMBRANES
PAGE 144
PROTEIN MARKERS PAGE 142
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74
I BLOTTING
omniPage
Mini Complete
Electroblotting
System
BLOTTING I 75
ORDERING INFORMATION
CVS10CBS
1x Mini Vertical Unit which includes: PAGE insert,
2x2mm thick notched glass plates, 2x2mm thick
plain glass plates with 1mm thick bonded spacers,
1x dummy plate, 2x combs (1mm thick 12 samples),
1x casting base, silicone mat, cooling pack; plus:
1x Mini Standard Electroblotting Module which
includes: Mini Platinum Wire Blotting Insert, 4x
10x10cm compression cassettes and 16x fibre pads
CVS10CBS-HI
The omniPAGE Mini electroblotting system allows PAGE and
blotting to be performed using the same universal tank and
lid. Interchangeable modules dedicated to PAGE or blotting
facilitate simultaneous transfer of up to four 10x10cm gels.
Features include:
• Transfer capability for up to four 10x10cm gels in as little
as an hour; low-intensity transfers may also be performed
overnight
• Platinum-wire electrodes 8cm apart generate a powerful
electric field for efficient transfer;
an optional plate-electrode insert is available for rapid
2-gel transfers
• Cool pack which, when frozen an hour before use, slots
conveniently into the tank to provide a low-cost, efficient
heat sink during fast high-intensity transfers
• Open design, rigid cassettes maximise current transfer
and eliminate ‘shadow band’ formation; colour-coded to
ensure proper orientation within the blotting system.
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Complete Mini System for electrophoresis and
blotting, comprising:
Complete Mini System for electrophoresis and high
intensity blotting, comprising:
1x Mini Vertical Unit which includes: PAGE insert,
2x2mm thick notched glass plates, 2x2mm thick
plain glass plates with 1mm thick bonded spacers,
1x dummy plate, 2x combs (1mm thick 12 samples),
1x casting base, silicone mat, cooling pack; plus:
1x Mini Standard Electroblotting Module which
includes: Mini Plate-electrode Blotting Insert, 2x
10x10cm compression cassettes and 8x fibre pads
VS10BI
1x Mini Blotting Insert, 4x compression cassettes and
16x fibre pads
VS10BI-HI
1x Mini High Intensity Blotting Insert, 2x
compression cassettes and 8x fibre pads
Technical Specifications
omniPage Mini
Wide Complete
Electroblotting
System
The omniPAGE Mini Wide electroblotting system shares
the same features as the omniPAGE Mini, to facilitate
simultaneous transfer of up to three 20x10cm Mini
Wide gels.
ORDERING INFORMATION
VS10WCBS
Complete Mini Wide System for electrophoresis
and blotting, comprising:
1x Mini Wide Vertical Unit which includes:
PAGE insert, 2x4mm thick notched glass
plates, 2x4mm thick plain glass plates with
1mm thick bonded spacers, 1x dummy plate,
2x combs (1mm thick 24 samples), 1x casting
base, silicone mat, cooling pack; plus: 1x
Mini Standard Electroblotting Module which
includes: Mini Platinum Wire Blotting Insert,
4x 20x10cm compression cassettes and 16x
fibre pads
Technical Specifications
Mini Wide
Maximum Gel
Size
17.5x8.5cm (active)
Maximum Gel Size 8x8.5cm (active gel dimensions)
Gel Capacity
6x Mini, 4x Mini Wide
Gel Capacity
4x CVS10 Mini Gels; 2 with plate-electrode insert
Buffer Volume
2.8L Max.
Buffer volume
1.2L Max.
Running
conditions
35V overnight to 100V high-intensity, <1-2 hours
Running
conditions
35V overnight to 100V high-intensity 1-2 hours
Recommended
power supply
CS-3AMP; CS-300V also recommended (Pg
87-88)
Recommended
power supply
CS-3AMP; CS-300V & CS-500V (Pg 87-88)
Mini
Typical Applications
Cast, Run and Blot up to 4 gels in the one system.
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76
I BLOTTING
BLOTTING I 77
ORDERING INFORMATION
Complete WAVE Maxi System for electrophoresis
and blotting, comprising:
VS20CBS
1x WAVE Maxi Vertical Unit which includes: PAGE
insert, 2x4mm thick notched glass plates, 2x4mm
thick plain glass plates with 1mm thick bonded
spacers, 1x dummy plate, 2x combs (1mm thick 24
samples), 1x casting base, silicone mat, cooling pack;
plus: 1x WAVE Standard Electroblotting Module
which includes: WAVE Maxi Platinum Wire Blotting
Insert, 4x compression cassettes and 18x fibre pads
VS20CBS-HI
The VS20WAVE
Electroblotting
System
Complete WAVE Maxi System for high intensity
electrophoresis and blotting, comprising:
1x WAVE Maxi Vertical Unit which includes: PAGE
insert, 2x4mm thick notched glass plates, 2x4mm
thick plain glass plates with 1mm thick bonded
spacers, 1x dummy plate, 2x combs (1mm thick
24 samples), 1x casting base, silicone mat, cooling
pack;
Tank Sub
Electroblotters
plus: 1x WAVE High Intensity Electroblotting
Module which includes: WAVE Maxi Plate Electrode
Blotting Insert, 1x compression cassettes and 6x
fibre pads
VS20BI
WAVE Maxi Platinum Wire Blotting Insert, 4x
compression cassettes and18x fibre pads – standard
VS20BI-HI
WAVE Maxi Plate Electrode Blotting Insert, 1x
compression cassette and 6x fibre pads – highintensity
Designed primarily for wet electroblotting of proteins,
TankSub Electroblotters offer a combination of increased
capacity with economy saving features. Both units, Mini 10
x 10cm and Maxi 20 x 20cm, have increased capacity over
standard systems with up to five gel blot cassettes utilised
at any one time. This is especially useful in high throughput
laboratories.
The VS20WAVE complete electroblotting system provides all
necessary components for performing transfers from vertical
slab gels.
features:
• Interchangeable modular inserts combine vertical PAGE
with electro-transfer using the same universal tank and lid
• Ideal for wet electroblotting of proteins Western blotting
• Multi-transfer capability for up to 4 WAVE Maxi gels, 8
omniPAGE Mini Wide and 16 omniPAGE Mini gels
• Complete flexibility with power settings as experimental
needs dictate: perform overnight transfer at voltages as
low as 35V; and rapid, high-intensity transfers at 200V in
1-2 hours
• Standard insert with platinum wire electrodes 8cm apart
for increased capacity; and a high-intensity insert with
plate electrodes 4cm apart for transfer rapidity
• Detachable cooling coil, which connects to the laboratory
water supply or a recirculating chiller, prevents buffer
depletion to allow overnight transfers and fast highintensity blots, and maintains the low temperatures
important for protein stability during native transfers
• Open design, rigid cassettes maximise current transfer
and eliminate ‘shadow band’ formation
• Colour-coded cassettes prevent polarity reversal
Typical Applications
Combine PAGE and transfer Techniques within the
same tank.
• Up to five gel blot cassettes utilised at any
one time
Technical Specifications
Maximum Gel
Size
Gel Capacity
Electrode types
17.5x18cm (active gel dimensions)
• Hinged cassettes for added convenience
• Accommodates gel thicknesses from 0.25
up to 3mm
Standard Blotting Insert 4x WAVE Maxi Gels
High-intensity Blotting
Insert
1x WAVE Maxi Gels
Standard Blotting Insert Platinum wire
ORDERING INFORMATION
High-intensity Blotting
Insert
EBM10
Electro Blot Mini, 10 x 10cm System for five cassettes, with tank
and lid, 5x cassettes, 24x fibre pads and cooling pack
Platinum-coated titanium
& stainless steel
Outer Tank
Buffer Volume
5.3L working volume to 6.4L max.
SB10C
Tank Blot Mini Cassette
Running
conditions
35V overnight to 200V high-intensity 1-2 hours
EBM20
Electro Blot Maxi, 20 x 20cm System for five cassettes, with
tank and lid, 5x cassettes, 24x fibre pads and cooling pack
Recommended
power supply
CS-3AMP (300 Volts, 3,000 mAmps, 300 Watts)
(Pg 88)
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SB20C
Tank Blot Maxi Cassette
SB10F
SB20F
Fibre pads - pk/8
Fibre pads - pk/6
A uniform electric field is provided by a high intensity
coiled electrode and ensures uniform transfer across the
blot surface. The cassette’s open architecture ensures the
maximum blot area allows direct transfer of current. Its
rigid construction ensures contact between the gel and
membrane is retained throughout the blot and an even
pressure is maintained. These units are compatible with
magnetic stirrers to aid heat dispersal and prevent pH drifts
in the buffer due to incomplete buffer mixing. Each system
includes a cooling pack to further enhance transfer efficiency
by removing excess heat. This also saves on buffer for added
economy.
Technical Specifications
Unit
dimensions
(w x h x d)
Mini
Maxi
24 x 16 x 26cm
Max. sample,
capacity
Mini
5 Blots, 10 x 10cm
Maxi
5 Blots, 20 x 20cm
19 x 13 x 19cm
20 Blots, 10 x 10cm
Buffer volume
Mini
Min 1000ml; Max 1500ml
Maxi
Min 4300ml; Max 6000ml
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78
I BLOTTING
BLOTTING I 79
Sub Blot Mini
and WAVE High
Intensity
Transfer Systems
omniPage Sub
Blot System
Available in Mini, Mini Wide and Maxi formats, omniPAGE
Sub Blot systems are complete stand-alone units for western
blotting. Each unit comprises an omniPAGE tank and lid,
electroblotting insert, cassettes and fibre pads. The versatile
design of the omniPAGE tank and lid allows the unit to be
easily adapted for vertical PAGE or IEF with capillary tube
gels, using the relevant optional insert.
ORDERING INFORMATION
SB10
SB10W
omniPAGE Blot Mini,
10 x 10cm System
including blotting
insert, tank and lid,
4 cassettes, 16 fibre
pads,cooling pack
omniPAGE Blot Mini
Wide,20 x 10cm System
including tank and lid, 4
cassettes, 16 fibre pads,
cooling pack
VS10BI
VS10WBI
features:
• Multi-transfer capability for up to 4 Maxi
gels, 8 Mini Wide and 16 Mini gels in the
Maxi System; 4 Mini Wide and 8 Mini gels in
the Mini Wide system; and 4 Mini gels in the
Mini system
• Complete run-time flexibility from overnight
to as short as a couple of hours
• Cool pack for environmentally friendly lowcost cooling during high-intensity transfers
• Open design, rigid cassettes maximise
current transfer and eliminate ‘shadow band’
formation
• Cassettes colour-coded prevent polarity
reversal to ensure transfer in the correct
orientation
ACCESSORIES
omniPAGE
Blot Mini Insert
–includes 4
cassettes and
16 fibre pads
Wet Western Electroblotting
omniPAGE Blot Mini VS20BI omniPAGE
Blot Maxi
Wide Insert –includes
WAVE Insert
4 cassettes and 16
–includes 4
fibre pads
cassettes and
16 fibrepads
SB10C
omniPAGE Blot
Mini Cassette
SB10WC
omniPAGE Blot Mini
Wide Cassette
SB20C
omniPAGE Blot
Maxi Cassette
SB10F
Fibre Pads –
pk/8
SB10WF
Fibre Pads – pk/8
SB20F
Fibre Pads –
pk/8
Technical Specifications
ORDERING INFORMATION
SB10-HI
omniPAGE Blot Mini, 10 x 10cm, High Intensity
System including tank and lid, 2 cassettes, 8 fibre
pads, cooling pack
VS10BI-HI
omniPAGE Blot Mini Insert – includes 2 cassettes
and 8 fibre pads
SB10C
omniPAGE Blot Mini Cassette
SB10F
Fibre Pads – pk/8
SB20-HI
WAVE Sub Blot, 20 x 20cm, High Intensity System
including tank and lid, 1 cassettes, 6 fibre pads,
cooling coil
VS20BI
omniPAGE Blot Maxi WAVE Insert –includes 4
cassettes and 16 fibrepads
SB20C
omniPAGE Blot Maxi Cassette
SB20F
Fibre Pads – pk/8
SB10 Mini
SB10W Mini Wide
SB20 MaxiWAVE
Max. Gel Size
8x8.5cm
16x8.5cm (active)
16x17.5cm
(active)
SB10-HI
SB20-HI
Max. Blot Size
10x10cm
20x10cm
20x20cm
Max. Gel Size
10x10cm
20x20cm
Gel Capacity
2x Mini
4x Mini, 2x Mini Wide &
1x Maxi
Buffer Volume
1.3L Max.
7L
Running conditions
100-200V 1-2h
100-200V 1-2h
Cooling
Cool Pack
Cooling Coil
Gel Capacity
Buffer Volume
Running
conditions
4x Mini
6x Mini, 3x Mini
Wide 12x Mini
16x Mini 8x Mini
Wide & 4x Maxi
1.2L Max.
2.8L Max.
6.4L Max
100V 1-2h
Recommended CS-3AMP &
power supply
CS-300V
Typical Applications
SB20
omniPAGE Blot
MaxiWAVE, 20
x 20cmSystem
including tank and
lid, 4 cassettes, 16
fibre pads, cooling
coil
100V 1-2h
100V 5-20h
CS-3AMP, CS300V & CS-500V
CS-3AMP
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Technical Specifications
Recommended power CS-3AMP
supply
CS-3AMP
Typical Applications
High Speed Wet Western Electroblotting
The Sub Blot Mini and WAVE high intensity transfer systems
combine the cooling capacity of wet submarine blotting
units with the speed of semi-dry transfer systems. Both
systems utilise plate electrodes to create a higher strength
electric field and greater current density than conventional
wire-electrode systems. This allows transfer of broad range
molecular weight proteins to be achieved typically within an
hour in the Sub Blot Mini, and in 1-2 hours using the WAVE
Sub Blot System. Features offered by the Sub Blot Mini and
WAVE are as follows:
• Plate-electrodes 2cm apart in the Sub Blot Mini facilitate
fast transfer of two stacked 8x8.5cm mini gels; and 4cm
apart in WAVE Sub Blot system for transfer of one Maxi
gel
• Multi-transfer capability in the WAVE also allows transfer
of 4 omniPAGE Mini and 2 omniPAGE Mini Wide gels
arranged side-by-side
• Two cooling options: a cool pack supplied with the Sub
Blot Mini for environmentally friendly low-cost cooling;
and active cooling in the Sub Blot WAVE by a detachable
cooling coil connected to a recirculating chiller (Pg 130)
• Magnetic stirring bars fit conveniently beneath the cooling
coil within the WAVE tank to maximise buffer circulation
and heat dissipation, while preventing ion gradient formation; both tanks sit comfortably on most stirring plates
• Open design, rigid cassettes maximise gel-to-membrane
compression for efficient current transfer; colour-coded to
prevent polarity reversal
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80
I BLOTTING
BLOTTING I 81
features:
•Rapid transfer times
•Western, Southern and Northern Blots
•Economic Transfers due to very low buffer volumes
•Screw down lid – gels from 0.25 up to 10mm thick
can be blotted
Semi Dry
Blotters
These Semi Dry Blotters offer rapid transfer times for DNA,
RNA and protein blotting – typically 15 to 30 minutes.
All units can be used for all types of blotting: western,
Southern and northern via uncomplicated buffer and set
up procedures and are compatible with gel thicknesses
from 0.25 up to 10mm without the need for additional
equipment. Each unit is compatible with their respective
omniPAGE vertical gel system.
Semi Dry Blotting has the added benefit of economic
transfers due to very low buffer volumes – typically only a
few millilitres of buffer are required per transfer. These SemiDry Blotters utilise a screw down lid, which secures the blot
sandwich and allows complete control of pressure ensuring
even transfer. The electrodes, comprising platinum coated
anode and stainless steel cathode, will exhibit practically no
corrosion and so provide many years of trouble free use.
Uniform heat dispersion across the blot sandwich ensures
stable transfer times and no heat induced sample loss or
transfer distortions. Being translucent, it allows viewing of
the blot sandwich to ensure correct positioning and transfer
is occurring correctly. Electrode plates are fully separated to
prevent arcing or damage.
Typical Applications
Rapid transfer of DNA, RNA or proteins from gels onto
membranes. Termed Southern, Northern and Western
blotting.
•Uniform heat dispersion
•Long life electrodes
features:
• Low cost
ORDERING INFORMATION
• Simple construction
SD10
Semi Dry Mini, 10 x 10cm System
• Easy assembly
SD20
Semi Dry Maxi, 20 x 20cm System
SD33
Semi Dry Maxi Plus, 33 x 45cm System
• Four sample configurations
SD50
Semi Dry Maxi Long, 20 x 50cm System
SD10-CS3AMP
D10 and cs-3amp power supply
SD20-CS3AMP
Sd20 and cs-3amp power supply
• Alpha-numeric sample identification
ORDERING INFORMATION
Technical Specifications
Unit
Mini
16 x 16 x 7cm
Maxi
26 x 26 x 7cm
Maxi Plus
33 x 45 x 7cm
CSL-D48
48-well Dot Blot
Manifold, 3 x 16 array
CSL-S24
24-well Slot Blot
Manifold, 2 x 12 array
CSL-D96
96-well Dot Blot
Manifold, 8 x 12 array
CSL-S48
48-well Slot Blot
Manifold, 3 x 16 array
Maxi Long 26 x 56 x 7cm
Max. sample
capacity
Mini
Maxi
Maxi Plus
1 Blot, 8 x 8.5cm
MODEL
4 Blots, 8 x 8.5cm,2 Blots, 16 x 8.5cm
1 Blot, 16 x 17.5cm
CSL-D48
20 Blots, 8 x 8.5cm, 2 Blots, 26 x 20cm
Configuration 3 x 16
10 Blots, 16 x 8.5m, 1 Blot, 33 x 45cm
Size of well
5 Blots, 16 x 17.5cm
13 Blots, 8 x 8.5cm, 2 Blots, 26 x 20cm
Maxi Long 6 Blots, 16 x 8.5cm, 1 Blot, 20 x 50cm
2 Blots, 16 x 17.5cm
Buffer volume Mini
5ml
Maxi
20ml
Maxi Plus
75ml
Maxi Long 50ml
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Vacuum
required
CSL-D96
CSL-S24
CSL-S48
8 x 16
2 x 16
3 x 16
6mm
6mm
6mm
6mm
diameter
diameter
diameter
diameter
12mm deep 12mm deep 12mm deep 12mm deep
----- 600mg Hg 0.8 BAR with cold trap -----
Unit
dimensions
60 x 95 x
100cm
60 x 105 x
140cm
60 x 74 x
83cm
60 x 95 x
100cm
Membranes
size required
12.1 x
4.4cm
11 x 7.4cm
12.1 x
4.4cm
12.1 x
4.4cm
Typical Applications
Direct transfer of Nucleic acid or protein samples onto
membranes for hybridisation or antibody screenings
Dot and Slot
Blotters
Four different sample number size and style of Hybridisation
Manifold are offered:- two types of dot blotter and two
types of slot blotter.
Typical applications include clone screening with DNA /
RNA probes in Southern/northern blots and immunological
screening with antibodies in western blots.
The units incorporate precision lapped surfaces to ensure
uniform blotting membrane contact and a leak proof
gasket. These prevent lateral transfer of samples – smudging
– by ensuring that a complete vacuum is formed. Six
thumbscrews ensure even and tight sealing for fast sample
suck down. Dot blotters are available in 48 and 96 well
versions and slot blotters in 24 and 48 well.
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omniPAC Power Supply Specifications
MINI
nanoPAC-300
Output range
Volts
Current
Power
Resolution
10-300V
10-400mA
60W max.
1V / 1mA
Type of output
MIDI
CS-300V
nanoPAC-500
10-500V
10-400mA
120W max.
1V / 1mA
MAXI
CS-500V
POWER
SUPPLIES
MAXI
CS-3AMP
2-300V
1-700mA
150W
1V / 1mA
5-500V
1-800mA
300W
1V / 1mA / 1W
5-300V
10-3000mA
300W
1V / 1mA / 1W
Constant voltage or constant current
Constant voltage or
constant current
Constant voltage, constant current or constant
power
Automatic crossover
–
4
4
Timer
1-999 min. with alarm;
Continuous
Constant mode: 1-9999 min. with alarm; con1-999 min. with alarm;tinuous.Programmable mode: 1-999 min. with
Continuous
alarm; continuous.
Pause/resume function
–
4
4
4
Display
3-digit LED
3-digit LED
2.6” LCD, 2-line
2.6” LCD, 2-line
Programmable
Methods
–
Automatic recovery
after power failure
–
Safety features
No-load detection; over-voltage, current &
temperature protection; maximum power output
detection; shrouded plugs and sockets
No-load detection; sudden load change detection; overload detection;
ground-leak detection; over- voltage, current & temperature protection;
maximum power output detection; shrouded plugs and sockets
Operating conditions
Ambient-40°C; ≤95% humidity
Ambient-40°C; ≤95%
humidity
Ambient-40°C; ≤95%
humidity
Ambient-40°C; ≤95%
humidity
Stackable
–
4
4
Number of output jacks 2 sets in parallel
4 sets in parallel
Regulatory conformity
EN-61010-1; CE
Dual voltage
Whether you require a power supply for routine horizontal
DNA agarose gel electrophoresis or techniques as technically
demanding as SSCP analysis within a large format vertical,
or first dimension IEF using IPG strips, Cleaver Scientific can
meet your requirements with its comprehensive range of
power supplies. Each power supply benefits from a small
footprint area and compact design, while explanatory selfprompting menus facilitate easy set-up. Furthermore, these
power supplies adhere to IEC 61010 – one of the world’s
most stringent electrical safety standards.
4
Up to 30 programmable files, each with 6 steps
4
4
4
4
Selection Guide
84-85
nanoPAC Power Supplies
86
4
omniPAC Power Supplies
87-88
4 sets in parallel
4 sets in parallel
Consort Power Supplies
89
EN-61010-1; CE
EN-61010-1; CE
EN-61010-1; CE
4
100-240 VAC
4
100-240 VAC
4
100-240 VAC
4
100-240 VAC
Construction
Polycarbonate housing with aluminium base
Flame retardant ABS-plate design with aluminium base
Dimensions (WxDxH)
140x191x84mm
190x305x95mm
190x305x95mm
190x305x95mm
Weight
1Kg
2.5Kg
2.5Kg
2.5Kg
1Kg
RELATED PRODUCTS
Horizontal
Gel tanks
PageS 4-45
Vertical
Gel tanks PageS 48-71
Blotting systems 2-D
PageS 72-81
Electrophoresis
PageS 63-64
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84
I POWER SUPPLIES
POWER SUPPLIES I 85
Power Supply Selection Guide
Gel or Tube Size*,
Technique &
Quantity
Apparatus
(Width x Length x
Format
Thickness)
Electrophoresis Power Supplies
Typical Running Conditions†
At start
End
Voltage
(V)
Power (W)
Current
(mA)
Run Time
Voltage
(V)
Power (W)
Current
(mA)
omniPAC
(Consort)
Power Supply
SDS-PAGE, second-dimension 2-D
VS30DSYS
280 x 200 x
1mm, 2 gels
–
100
35 (Constant)
–
350
35 (Constant)
5½-6h Max
CS-500V
VS20WAVE
160 x 175 x
1mm, 2-4 gels
–
100
35 (Constant)
–
350
35 (Constant)
5h Max
CS-500V or
nanoPAC-500
VS10WDSYS
160 x 85 x
1mm, 2 gels
–
200
(Constant)
200
–
200
(Constant)
80
60-80 min
CS-300V,
nan PAC-300
or -500
80 x 85 x 1mm,
2-4 gels
–
200
(Constant)
120-240
200
(Constant)
120
CVS10DSYS,
CVS10TETRAD,
CVS10TETPRO
–
40-60 min
–
Maxi Tube Gel – 180 x 1/1.5mm
tubes, 10 max.
e.g. VS20DC,
WAVEC2DS
–
800
(Constant)
4
–
800
(Constant)
<1
8h Max
EV215
–
700-800 (Constant)
1
–
700-800 (Constant)
<1
4h Max
EV215
80 x 1/1.5mm
tubes
Mini Tube Gel
- e.g. VS10DC,
CVS10C2DS
80 x 1/1.5mm
tubes
Current (mA)
Power (W)
Voltage (V)
Current (mA)
100 x 120 x 5mm
–
70 (Constant)
40
–
80 (Constant)
45
30-45 min
nanoPAC-300,
-500 or CS-300V
MSMIDI96ST
100 x 240 x 5mm
–
90 (Constant)
50
–
95 (Constant)
55
60-90 min
nanoPAC-300,
-500 or CS-300V
MULTISUB4
80 x 240 x 5mm, max.
–
90 (Constant)
50
–
95 (Constant)
55
60-90 min
nanoPAC-300,
-500 or CS-300V
MSCHOICEST
150 x 250 x 5mm, max.
–
90-150 (Constant)
50-80
–
90-150 (Constant)
55-90
60-90 min
nanoPAC-300,
-500 or CS-300V
MSSCREENTRIO
260 x 320 x 5mm, max.
–
100-150 (Constant)
50-80
–
100-150 (Constant)
55-90
90-120 min
nanoPAC-300,
-500 or CS-300V
–
25V (Constant)
300 max.
–
25V (Constant
300 max.
1h
nanoPAC-300,
-500 or CS-300V
21V (Constant)
450 max
21V (Constant)
20 mins
CS-300
–
200V (Constant) 7.5 max.
30-90 min
nanoPAC-300,
-500 or CS-300V
Comet Assay, SCGE (Horizontal)
CSL-COM10
CSL-COM20
CSL-COM40
COMPAC-50™
25 x 75mm, 10, 20, 40 &
50 slides respectively
Clinical Electrophoresis (Horizontal)
CSL-CELLAS
25 x 140mm–170 x
170mm, Cellasgel strips
max. 250μM thickness; or
CellasMEM membranes
(all types)
–
200V (Constant) 7.5
DNA Sequencing, SSCP Analysis & Microsatellite Mapping (Large Format Vertical)
3 x 240 x 1mm,
max.
12 strips
Mini-Wide
TubeGel - e.g.
VS10WDC,
VS10WC2DS
Voltage (V)
300
(Constant)
3
–
300
(Constant)
<1
16h Max
EV233
CSQ20
160 x 500 x 0.35mm
45-55
1500 max.
(Constant)
20-30
45-55
(Constant)
1500
20-30
4-5h
EV233
CSQ33
290 x 410 x 0.35mm
45-55
1500 max.
(Constant)
20-30
45-55
(Constant)
1500
20-30
4-5h
EV233
160 x 175 x 1mm, 2 gels
–
120-150
(Constant)
–
–
120-150
(Constant)
–
2-2.5h
CS-500V
omniBLOT Mini
– e.g. SB10
80 x 85 x 1mm, 4 gels
–
100 (Constant)
250
–
100 (Constant)
400
1-2h
CS-300V or
CS-3AMP
Modular
System - e.g.
CVS10CBS
80 x 85 x 1mm, 4 gels
–
100 (Constant)
250/550 Wire/
Plate
Electrodes
–
100 (Constant)
400/1500
Wire/Plate
Electrodes
2h / ½-1h
Wire/Plate
Electrodes
CS-300V/
CS-3AMP
Wire/Plate
Electrode
Standalone –
e.g. EBM10
80 x 85 x 1mm, 4 gels
–
100 (Constant)
250
–
100 (Constant)
400
1-2h
CS-300V or
CS-3AMP
omniBLOT Mini
Wide – e.g.
SB10W
160 x 85 x 1mm, 3 gels
–
100 (Constant)
250
–
100 (Constant)
250
1-2h
CS-300V, CS-500V
or CS-3AMP
Modular
System - e.g.
VS10WCBS
160 x 85 x 1mm, 3 gels
–
100 (Constant)
250
–
100 (Constant)
250
1-2h
CS-300V, CS-500V
or CS-3AMP
WAVE Maxi
– e.g.
VS20CBS
160 x 175 x 1mm, 4 gels
–
50/100 Wire/
Plate Electrodes
(Constant)
150-250/10001600
–
50/100 Wire/
Plate Electrodes
(Constant)
150250/10001600
5-20h/
1-5h
CS-3AMP
omniBLOTMaxi
– e.g. SB20
160 x 175 x 1mm, 3 gels
–
50 (Constant)
250
–
50 (Constant)
250
5-20h max.
CS-3AMP
Mutation Detection
VS20-DGGE
Western Blotting
–
700-800 (Constant)
1
–
700-800 (Constant)
<1
4h Max
EV215
DNA Restriction Analysis (Horizontal)
MSMINIDUO
70 x 100 x
5mm, max.
–
80 (Constant)
40
–
80 (Constant)
45
45-60 min
nanoPAC-300,
-500 or CS300V
MSMIDIDUO
100 x 100 x
5mm, max.
–
90 (Constant)
50
–
95 (Constant)
55
45-60 min
nanoPAC-300,
-500 or CS300V
FMMS10
omniPAC (Consort) Power
Supply
MSMIDI96
CS-300V,
nanoPAC-300
or -500
IEF, first-dimension 2-D
Flat-bed
e.g. CSL-IEF
Power (W)
Run Time
End
High Throughput DNA Electrophoresis (Horizontal)
Please use the selection menu below to choose the power supply most suitable for your electrophoresis application. The typical
running conditions shown serve as guidelines only.
Gel or
Tube Size*,
Technique &
Quantity
Apparatus
(Width x
Format
Length x
Thickness)
Typical Running Conditions†
At start
100 x 80 x 5mm –
MSCHOICETRIO
150 x 150 x
5mm, max.
–
MSMAXIDUO
200 x 200 x
5mm, max.
–
50 (Constant)
25
90-150 (Constant)
50-80
100-150 (Constant)
50-80
–
–
–
50 (Constant)
35
90-150 (Constant)
55-90
100-150 (Constant)
55-90
30-60 min
60-90 min
60-90 min
nanoPAC-300,
-500 or CS300V
nanoPAC-300,
-500 or CS300V
nanoPAC-300,
-500 or CS300V
*Sizes shown are those most commonly used in the corresponding apparatus. See product manuals for running conditions for additional sizes.
†(Constant) the parameter set as a constant value on the power supply. Typical conditions are to serve as guidelines only, and will vary according to the
buffer and overall quality of the sample and reagents. a Uses 2xTAE.
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Semi-Dry Blotting (Protein / Nucleic Acids)
SD10 Mini
100 x 100 x 2/5mm, 1 gel
–
75 (Constant)
550
–
75 (Constant)
550
15-30 min
CS-3AMP
SD20 Maxi
200 x 200 x 2/5mm, 1gel;
4x Mini Gels
–
75 (Constant)
1200
–
75 (Constant)
1200
15-30 min
CS-3AMP
SD33 Maxi-Plus
330 x 450 x 2/5mm, 1gel;
14x Mini Gels; 3x Maxi
Gels
–
75 (Constant)
2000
–
75 (Constant)
2000
15-30 min
CS-3AMP
SD50 MaxiLong
200 x 500 x 2/5mm, 1
gel; 10x Mini Gels; 2x
Maxi Gels
–
75 (Constant)
2000
–
75 (Constant)
2000
15-30 min
CS-3AMP
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86
I POWER SUPPLIES
POWER SUPPLIES I 87
omniPAC Midi
CS-300V
nanoPAC-300
& 500 Power
Supplies
features:
The nanoPAC Mini Power supplies are an ultra compact
and economic unit ideal for use with DNA (Horizontal) and
Protein (Vertical) electrophoresis systems.
• 500Volts, 400mAmps, 120 Watts For DNA &
Protein
With enhanced features, such as a maximum constant
voltage up to 300 or 500V and maximum constant current
output of 400mA the nanoPAC’s are capable of running all
Cleaver Scientific horizontal multiSUB™ systems and vertical
omniPAGE™ mini. The nanoPAC-500 is also capable of
running the VS10W & VS20WAVE vertical units, as well as
horizontal and vertical gel tanks from other manufacturers,
These can be set on a continuous run or timed setting up to
999 minutes. The nanoPAC’s user-friendly interface is easily
adjustable in 1V and 1mA increments, making it perfect
for separations where precise settings are required. Its ultra
compact size and two pairs of parallel power terminals,
which can run two electrophoresis units simultaneously, save
time and bench space.
ORDERING INFORMATION
nanoPAC-300
Mini Power supply, 300V, 400mA, 60W - 100240VAC
nanoPAC-500
Mini Power supply, 500V, 400mA, 120W 100-240VAC
Typical Applications
NanoPAC-300 - Suitable for running all Cleaver Horizontal
Gel tanks and the CVS10 Mini Vertical. NanoPAC-500 - as
above but also larger vertical systems such as VS20WAVE.
• Highly visible LED display
• 300Volts, 400mAmps, 60 Watts For DNA &
Protein
• Electrophoresis Enhanced in-built safety
features Alarm function
• Easy maintenance and cleaning
OUTPUT SPECIFICATIONS
Order No.
nanoPAC-300
nanoPAC-500
Output Voltage / Inc.
0-300V /1V
10 - 500V / 1V
Output Current / Inc.
10 - 400mA / 1mA
Output Power
60W
Output Type
Constant voltage or constant current
Control
Microprocessor controller
Terminal Pairs
2 Pairs
Timer
1~999 minutes with alarm or continuous
Safety Device
No load detection; shrouded plugs and sockets
Unit Dimension (W x
L x H)
140 x 191 x 84mm
Weight
Approx. 1 kg
Rated Voltage
120W
Universal, 100 - 240V
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
ORDERING INFORMATION
CS-300V
omniPAC MIDI Power Supply, 300V, 700mA,
150W – 100-240VAC
OUTPUT SPECIFICATIONS
With nearly twice the current and power of the market
leader’s equivalent unit, at 700mA and 150W, the CS300V offers a specification comparable to any midi power
supply presently available on the market. The CS-300V is
perfectly suited to use with all Cleaver Scientific horizontal
multiSUB™ systems and omniPAGE mini vertical gel units,
and may also be adapted for specialist techniques including
the Comet Assay, and clinical and high throughput horizontal electrophoresis. Microprocessor control with four sets of
power terminals allow simultaneous operation of as many
electrophoresis units either at a constant voltage or current
setting, while the timer function may be set continuously
or up to a maximum 999 minutes when an alarm sounds
to signify termination of the run. A user-friendly interface
houses a conspicuous 3-digit LED to aid set up, as well as a
convenient ‘pause/resume’ key, a particularly useful feature
during extended runs when it is necessary to access the gel
tank to monitor buffer levels and sample migration. Given its
high specification and remarkable versatility the CS-300V is
relatively inexpensive, and benefits from additional features
such as:
• a stackable design;
Voltage
2-300V
• rigorous in-built safety mechansims;
Current
1-700mA
• automatic crossover;
Power
150W max.
• dual voltage compatibility.
Typical Applications
General Laboratory power supply ideal for DNA gels and
mini protein electrophoresis and blotting.
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
88
I POWER SUPPLIES
POWER SUPPLIES I 89
Consort Power
Supplies
omniPAC
Maxi CS-500V
omniPAC
Maxi CS-3AMP
With a maximum 500V, 800mA and 300W voltage, current
and power output, the omniPAC CS-500V MAXI is an excellent
general purpose power supply that fits the broadest range
of electrophoresis applications, and can run as many as four
units under a constant or programmable setting. Its capacity to
store up to 30 programmed files, each with 6 steps, makes the
CS-500V perfect for techniques that benefit from subtle and
gradual stepwise changes in the electrical parameters as the
run progresses, such as DGGE and large format vertical PAGE
using Cleaver Scientific’s VS20-DGGE and WAVE systems.
A large 2.6” LCD screen shows within 2 lines the real-time
values of the electrical parameters during the run, along with
the program file and individual step in operation, enabling the
user to appraise the entire run at a single glance. The CS500V MAXI is also equipped as standard with many features
associated with the omniPAC MIDI, including timer and
alarm, ‘pause / resume’ functionality, a stackable design and
enhanced in-built safety features.
At 300V, 3000mA, 300W, the omniPAC MAXI CS-3AMP
is designed for virtually all high current electrophoresis
applications. The CS-3AMP’s higher current output capability
is perfect for electroblotting units with high-intensity plate
electrodes, particularly Cleaver Scientific’s omniBLOT maxi,
VS20 WAVE and semi dry blotting systems. Electrotransfers
may be performed as timed runs in constant or programmable
mode to prevent overheating and buffer depletion, although a
run time extendable to a maximum 9999 minutes in constant
mode also favours overnight transfers undertaken at constant
low current in wire electrode systems. The two-line 2.6” LCD
screen allows the experimental parameters, program file and
step to be viewed within a single screen during each run, while
the CS-3AMP’s four power terminals and robust current and
power outputs make it suitable for high throughput SDS-PAGE
using multiple vertical systems. The CS-3AMP shares the same
standard features as the omniPAGE MAXI CS-500V.
All Consort Maxi Series (EV200) power supplies have four
output terminals for up to four simultaneous runs. Powerful
microprocessor control allows complex programming, while
manual mode permits the setting of voltage, current, power
and time for routine electrophoretic runs. The parameters may
also be changed temporarily without interrupting the run.
Programming – up to 9 different programs, each with 9 steps,
can be stored in the non-volatile memory for future recall.
Timer – can be set to automatically terminate the run and
sound an alarm when the allocated time or volt hours has
elapsed.
Automatic cross-over – each model has constant voltage,
constant current and constant power capabilities with
automatic cross-over while showing which parameter is kept
constant.
Automatic recovery after mains failure – after restoration of
power, the instrument will automatically continue the run for
the remaining time.
omniPAC MAXI Power Supply, 500V, 800mA,
300W – 100-240VAC
CS-3AMP
omniPAC MAXI Power Supply, 300V, 300mA,
300W – 100-240VAC
OUTPUT SPECIFICATIONS
Typical Applications
More advanced programmable power supplies with
increased voltage, power and current
The Consort Mini EV245 power supply is ideally suited for
use with small electrophoresis systems. It occupies little
space within the laboratory, is portable and has several other
features:-
Data Transfer – optional data acquisition/control software for
PC is available to visualise and examine the stored run details,
store and program the methods, add notes to the run, identify
the unit, copy or delete programs in the unit’s memory.
Manual Set-up – voltage, current and power settings can be
adjusted during the run.
Safety precautions – the AC line is automatically disconnected
from the high voltage transformer when a ground leakage
path is detected protecting the user from potential shock.
Other safety features include protection against any overload
including accidental short circuit of the output and voltage
increases smoothly.
Automatic recovery after power failure.
Voltage ramp – this allows a linear voltage gradient for any
step to be programmed at any step.
3 output terminals.
The EV245 also benefits from 9 different programs, each
with 9 steps; data-logging and data transfer to a PC, and PC
remote control.
ordering information
ORDERING INFORMATION
CS-500V
Consort Mini
EV245
TECHNICAL SPECIFICATIONS
EV245
Consort Power Supply 400 V 300 mA 50 watts
EV231
Consort Power Supply 300 V 1000 mA 150 watts
EV202
Consort Power Supply 300 V 2000 mA 300 watts
EV265
Consort Power Supply 600 V 500 mA 150 watts
Consort Maxi
Consort Mini
Timer
0-99.59 hours
0-99.59 hours
Programs
9 of 9 sets each
EV243: 9 of 9 sets each
EV261
Consort Power Supply 600 V 1000 mA 300 watts
Outputs
4 in parallel, 4mm sockets
3 in parallel, 4mm sockets
EV215
Consort Power Supply 1200 V 500 mA 300 watts
1V, 1mA, 1W
EV233
Consort Power Supply 3000 V 300 mA 300 watts
Consort Power Supply 6000 V 150 mA
EV222: 1 set
CS-500V
CS-3AMP
Voltage
5-500V
5-300V
Resolution
1V, 1mA, 1W
Current
1-800mA
10-3000mA
Dimensions
31 x 26 x 15cm (w x l x h) 24 x 20 x 13cm (w x l x h)
EV262
Power
300W max.
300W max.
Weight
10kg max.
For 110V add $ to stock code.
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
3kg max.
Typical Applications
Suitable for powering different gel tanks depending on size
300 watts
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
GEL
DOCUMENTATION
GEL DOCUMENTATION OVERVIEW
Gel documentation is usually performed
in molecular biology laboratories whereby
nucleic acid and protein gels are imaged and
then documented and the DNA, RNA or
protein bands are identified, quantitated and
the results analysed. This is an important
step in many molecular biology techniques
and studies including cloning, mutation
analysis, protein identification, quantitation
and expression. Such techniques are
important stages in studies in infectious
diseases and cancer, drug development,
human genome analysis, cell structure
and function and studies on food and the
environment.
Originally Universities and research institutes
had a dark room within their departments
with a UV light source for ethidium bromide
stained DNA gels or with white light boxes
for Coomasie stained protein gels and
a simple Polaroid camera set up to take
pictures and produce a photo of the gels to
be stuck into lab books.
Gel documentation technology has since
been revolutionised with much more sensitive
camera systems with independent dark room
cabinets and computer gel analysis software
to document the results and allow in-depth
analysis of the bands or spots on the gels.
These also allow the simple generation of
publication quality images for sharing of
results in scientific journals.
Consequently, gel documentation is now
applicable to routine horizontal DNA
agarose gels, fluorescent sequencing gels,
preparative gels, colorimetric protein gels,
and chemiluminescent blots. By using
modern imaging and excitation technologies
gel documentation systems are now more
reliable, accurate and quantitative than ever
before and can be adapted for almost any
stain, dye, probe, antibody or fluorescent
protein.
Selection Guide
92-93
microDOC™94-95
omniDOC and omniDOC-i
Cleaver Scientific provides a comprehensive
range of gel documentation and imaging
systems to suit any application or budget.
Our range starts with the popular
microDOC™. Low cost systems which are
ideal to image routine horizontal DNA or
protein gels.
96-99
Uvitec Gel Documentation Systems
100
UV Crosslinker
101
UV and Blue Light Transilluminators
microSCAN Scanner
102-104
105
Gel Analysis Software including 2-D
106-109
The new omnidoc systems contain a
highly sensitive camera and have convenient
features such as quick light source switching
for DNA and protein gels and wireless
downloading of images to PC, Laptop or
smart device.
We offer the full range of UVItec
systems which include high specification
models dedicated to Gel documentation,
chemiluminescence and fluorescence.
Finally we offer a full range of accessory
products including scanners, light sources,
crosslinkers and one and two dimension gel
software.
RELATED PRODUCTS
Horizontal Gel
Systems
PAGES 4-45
Vertical Gel
Systems
PAGES 46-71
Blotters
PAGES 72-81
2-D
Electrophoresis
PAGE 63
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92
I GEL DOCUMENTATION
GEL DOCUMENTATION I 93
Gel Documentation Systems
Selection Guide
BASIC
VERSATILE YET AFFORDABLE
STRICTLY PROTEIN
ADVANCED
Auxiliary Products
DUOVIEW UV/BLUE LIGHT TRANSILLUMINATOR
The duoVIEW transilluminator combines blue light epi-illumination with UV transillumination. Blue light excitation eliminates concerns over user safety and minimises damage to nucleic acid samples, which compromises cloning efficiency.
The duoVIEW’s parallel configuration of two powerful LED arrays produces more uniform excitation than conventional
transilluminators, thus enabling gels containing SYBR Safe, SYBR Green and our own proprietary runSAFE stains to be
viewed at greater intensity. The duoVIEW also allows the user to switch between two UV wavelengths, at high or low
intensity, to view UV fluorescent gels; and may be used as a blue light transilluminator for the microDOC™.
SAFEVIEW TRANSILLUMINATORS
Like the duoVIEW, the safeVIEW blue light transilluminator also has a parallel configuration of two powerful LED arrays to
produce more uniform excitation than standard transilluminators. This enables gels containing blue-light fluorescent stains
to be viewed at greater intensity. The safeVIEW may be used either as a standalone blue light transilluminator or with the
microDOC™ as part of a complete gel documentation system.
Single/Dual Wavelength Transilluminators
Available in single and dual wavelength formats and in 21x21cm or 21x26cm sizes, Cleaver Scientific transilluminators
may be supplied individually or with the microDOC™ as part of a complete gel documentation system to visualise UV
fluorescent gels.
The microDOC™ and microDOC-BASIC are for entry-level and
basic application use. Both systems
are ideal for nucleic acid agarose
gels stained with ethdium bromide,
or runSAFE or SYBR green, and may
be used with a white light converter
or box for 1D colorimetric protein
gel imaging.
Although functional in design
to address modest budgets, the
microDOC™ and microDOCBASIC do not lack in specification
or performance. Both systems are
manually operated and supplied
with a high resolution colour
digital camera. The microDOCBASIC comprises a fully enclosed
ebony acrylic dark room that
may be placed directly on a UV
transilluminator or white light
box; whereas the microDOC™
includes an epoxy-coated dark room
with front door panel and safety
interlock switches that disconnect
the optional UV transilluminator
upon opening, while a large front
TFT screen aids visualisation of the
gel. Optional 1D analysis software
is available.
The omniDOC and omniDOC-i
offer high performance gel
documentation and analysis at a
relatively low cost. By providing
many of the features used by
leading gel documentation brands,
each omniDOC system presents a
simple but sophisticated imaging
solution for most laboratories. A
high resolution 5 mega pixel camera
with slide-out UV transilluminator,
and optional blue epi-illumination
module and white light table, makes
both systems suitable for imaging
most fluorescent and colorimetric
gels.
In the omniDOC a USB port
connects the dark room assembly to
an external PC for control, whereas
for the omniDOC-i a front panel
touchscreen may be used for image
acquisition and auto exposure.
Complimentary acquisition and
analysis software is supplied on a
disc for the omniDOC, or as an App
downloadable to a tablet or laptop
for remote image analysis from the
omniDOC-i.
Both systems benefit from a prefocused camera that requires little
or no manual adjustment and four
filter options for different fluorescent
applications.
The microSCAN is an affordable
low-cost gel documentation system
for scanning colorimetric 1-D and
2-D protein gels, as well as blots and
TLC plates.
Control software allows rapid
generation of high quality images
that may be then imported into
analysis software, such as TotalLab
Quant or Phoretix 1D. Users may
choose between transmittance and
reflectance modes in colour and
greyscale, and select their required
resolution to a maximum 2400 dpi.
The advanced gel documentation
systems of Uvitec cover a much
broader range of imaging
applications to complement
the existing gel imaging
capabilities offered by the Cleaver
Scientific range. Each Uvitec gel
documentation system features a
high performance CCD camera with
remarkable sensitivity to generate
high resolution images of even the
faintest gels or blots, while optional
dedicated epi-illumination modules
provide the excitation for near
infrared (NIR), red, green and blue
(RGB) imaging applications.
‘One-touch’ image acquisition
software simplifies the gel
documentation process by fully
automating lens positioning, filter
selection and exposure to allow
quantitative results to be obtained
quickly and easily.
In addition to 1-D and 2-D nucleic
acid and protein gel imaging,
Uvitec systems may be supplied
to perform chemiluminescence,
coloured fluorescence, multi-plex,
and biofluorescence and thin layer
chromatography applications.
UV-TO-WHITE LIGHT CONVERTER PLATES
For use with UV transilluminators, allow visualisation of colorimetric protein gels, blots developed on X-ray film, and
microbial colonies.
ANALYSIS SOFTWARE
OmniDOC Software – complimentary basic image analysis software supplied with all omniDOC systems for molecular
weight or size determination in 1-D gels
TotalLab 1D for 1-D gel analysis of nucleic acid and protein gels; TotalLab Quant includes an additional colony counting
module. Phoretix 1D, which includes TotalLab Quant, is a more sophisticated option for band-pattern matching within
individual gels, while Phoretix 1D Pro has added database functionality to generate and manipulate large lane comparison
datasets from multiple gels.
UVI 1D supplied with each Uvitec system includes molecular weight and volume calculation, and Rf and colony counting
functionalities. Uviband is fully automated and more sophisticated, with modules for 1-D molecular weight and optical
density; 1-D lanes / free form optical density; array optical density and colony counting.
SDS-PAGE, second-dimension 2-D
APPLICATION GUIDE
microDOC-BASIC
microDOC™
omniDOC
microSCAN
UVITEC
Fluorescence, UV
4 (Option A)
4 (Option A)
4 (OMNIDOC/-I)
–
4 (FIREREADER, PLATINUM, ALLIANCE)
Visible Stain, White Light
4 (Option B)
4 (Option B)
4 (OMNIDOC3/-I3)
(Option E)
4 (FIREREADER, PLATINUM, ALLIANCE)
Fluorescence, Blue Light
4 (Option C)
4 (Option C)
4 (OMNIDOC2/-I2)
–
4 (FIREREADER, PLATINUM, ALLIANCE)
Colony Plate
4 (Option D)
4 (Option D)
4 (OMNIDOC /-I + Option E) –
4 (FIREREADER, PLATINUM, ALLIANCE)
Dot Blots & Gel Blots, X-ray film
4 (Option D)
4 (Option D)
4 (OMNIDOC3/-I3
4 (Option E)
4 (FIREREADER, PLATINUM, ALLIANCE)
Fluorescence, RGB Colour
–
–
–
–
4 (ALLIANCE CHROMA)
Fluorescence, NIR
–
–
–
–
4 (ALLIANCE CHROMA)
Proteomics, 2-D
–
–
–
4 (Option F)
4 (PLATINUM, CHROMA)
Chemiluminescence
–
–
–
–
4 (ALLIANCE)
3
3
Option A: requires UV transilluminator + TotalLab1D Software
Option D: r equires UV transilluminator + UV-to-white light converter plate + TotalLab
Quant software
Option B: r equires UV transilluminator + UV-to-white light converter plate + TotalLab1D
Software
Option E: requires TotalLab Quant software
Option C: requires blue light / DUOVIEW transilluminator + TotalLab1D Software
Option F: requires Phoretix 1D software
Options (A-D) include the required transilluminator for gel imaging and recommended analysis software option; Options E&F show recommended analysis software only.
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94
I GEL DOCUMENTATION
GEL DOCUMENTATION I 95
Technical Specifications
Camera:
Type
1/1.7 type CMOS sensor with DIGI6 processor
Zoom
5x optical / 4x digital
Effective Pixels
12.1 megapixels
Max. Aperture
f/2.8 (W) - f/4.5 (H)
Shutter Speed
250 - 1/4000s. (total range)
Filters
+3 Close up and EtBr; optional SYBR green
Storage Media
4GB memory cardwith built in Wi-Fi
Computer Interface Hi-Speed USB (Mini-B compatible)
Video Out
NTSC/PAL
Darkroom:
Multi-Power Source For camera, inner white LED, TFT screen
Inner White Light
2x3W LED
Safety Device
Safety door switch
Weight
6.1kg
Dimensions
29x22x32cm
Voltage Rating
110~220V
Screen:
Type
8” TFT
Resolution
600x800 Pixels
Brightness
350 cd/mm2
Constant Ratio
300 : 1
Display Mode
NTSC / PAL / SECAM mode, auto switching
ORDERING INFORMATION
Gel Documentation
Systems
System only
Including TotalLab 1D
Analysis Software
Compact Gel
documentation system
CSL-MICRODOC
CSL-MICRODOC1D
microDOC™ System
CSLwith UV Transilluminator
MDOCUV312
(UVT312)
CSL-MDOCUV3121D
microDOC™ System
CSLwith UV Transilluminator
MDOCUV254
(UVT254)
CSL-MDOCUV2541D
microDOC™ System
CSLwith UV Transilluminator
MDOCUV365
(UVT365)
CSL-MDOCUV3651D
microDOC™ System
CSLwith UV Transilluminator MDOCUV254
(UVT254/312)
/312
CSL-MDOCUV254
/3121D
microDOC™ System
CSLwith UV Transilluminator MDOCUV254
(UVT254/365)
/365
CSL-MDOCUV254
/3651D
microDOC™ System
CSLwith UV Transilluminator MDOCUV312
(UVT312/365)
/365
CSL-MDOCUV312
/3651D
microDOC™ Basic
System with lift-off dark
room hood and camera
only
CSL-MDOCBASIC CSL-MDOCBASIC1D
microDOC™ System for
protein gels
CSL-MDOCWLB
microDOC™ Basic
System for protein gels
CSLCSLMDOCBASICWLB MDOCBASICWLB1D
microDOC™ System
for Colony Counting
(includes Totalab Quant
software)
microDOC™ Basic
System for Colony
Counting (includes
CSL-MDOCWLB1D
CSL-MDOCCOL
CSLMDOCBASICCOL
Totalab Quant software)
Accessories
Mitsubishi Thermal
Printer for use with
MicroDOC™ 110 - 240V
CSL-PRINT
MicroDOC™ ethidium
bromide filter
CSL-MDOCEB
MicroDOC™ SYBR filter CSL-MDOCSBRG
Replacement printer
CSL-PRTPAP
paper
UV to white light
conversion screen for
CSL-UVSCRN
transilluminator for
protein gels
White light box for Micro
CSL-MDOCWLB
doc for protein gels
Camera specification may change.
Optional 4GB wireless
memory card
features:
• 12.1 Megapixel
digital camera
with improved
high-sensitivity
CMOS sensor and
processor*
• Image visualised
within a large 8”
TFT colour monitor
• Light weight
compact hood with
easy access door
and built-in inner
lights
• Safety switches
disconnect the UV
source when the
easy access door is
opened
• Computer-free
operation
• Available on its
own with camera
and darkroom, or
as a complete gel
documentation
system with
transilluminator,
either with or
without software
• Transilluminators
supplied in
single and dual
wavelength formats
- see page 102
• Optional TotalLab™
1D analysis
software and
wireless memory
card
Gel Documentation
microDOC™
Now upgraded with a new 12.1
megapixel digital camera*, with
improved high-sensitivity CMOS
sensor and the latest image processor
to guarantee superb resolution, the
microDOC™ is the researcher’s
choice for a simple ultra-compact gel
documentation system that meets
constraints in both budget and space
within the laboratory.
A large 8” TFT screen enables images,
including agarose and fluorescent gels,
colorimetric gels, autoradiography film
and blotting membrane, to be captured
in colour, clearly and easily. The system
is computer-free and supplied with a
8GB storage card and 55mm ethidium
bromide filter as standard, while an
optional SYBR filter is also available.
Files are saved onto the 8GB storage
card in RAW, and JPEG formats and
may be transferred to computer for
analysis with the highly recommended
TotalLab™ 1D software (Pg 107).
* At the time of publication.
CSL-MDOC-WIFI
Printer
The Mitsubishi P93 is a high speed,
high resolution thermal printer that
is perfectly suited to printing images
directly from the microDOC™.
Connected to the microDOC™ by a
BNC cable, the Mitsubishi P93 prints
325dpi images, up to 133 x 99mm
in size. Thermal printer paper is also
available.
Wireless transfer
The new microDOC™ system includes
convenient wireless transfer of images
to a PC or smart device – no cable
connection required.
microDOC™ BASIC
The microDOC™ BASIC is a simple
low-cost system comprising a lift-off
dark room hood and 12.1 megapixel
digital camera, through which the gel
is viewed directly. This system can be
supplied with optional TotalLab™ 1D
Analysis Software and any one of the
21x21cm transilluminators listed on
Pg 102-103.
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Imaging, documentation and analysis
of DNA, RNA and Protein gels.
CSL-MDOCBASIC
CSL-MICRODOC
96
I GEL DOCUMENTATION
GEL DOCUMENTATION I 97
omniDOCi
3.5” 64K colour TFT display
shows at a glance the
excitation source in use,
and provides full manual
touchscreen control of the
excitation source, UV intensity
and exposure time.
OMNIDOC features:
• Pre-focused 5 mega pixel camera with
auto-exposure for almost instantaneous high
resolution gel imaging; CMOS sensor for
improved light sensitivity
• 6mm lens, F1.2 aperture size, with manual
adjustment
• Interchangeable filter slide with 620nm
ethidium bromide filter as standard; 520,
560 and 580nm filter options available for
runSAFE, SYBR stain and other fluorescence
applications
• Viewing pane with universal amber filter for
gel inspection, which may be covered by a
spring-loaded panel during documentation
• Internal white LED – aids gel positioning and
focusing
• Slide-out 312nm transilluminator; uses
optional plug-in white light table for
coomassie, silver-stain and other colorimetric
gels
• Large 21x26cm filter area
omniDOC and
omniDOCi
omniDOCi wireless remote control possible through
multiple portable devices
OMNIDOCi features:
Two new systems for affordable, high
performance gel documentation and analysis
omniDOC
The omniDOC is the first of two new systems from Cleaver
Scientific offering high performance gel documentation and
analysis at a relatively low cost. By providing many of the
features used by leading gel documentation brands, but
without the added price premium, each omniDOC system
presents a simple but sophisticated imaging solution for
most laboratories. A high resolution 5 mega pixel camera
with slide-out UV transilluminator, and optional blue epi-illumination module and white light table, makes the omniDOC
suitable for imaging most fluorescent and colorimetric gels,
while a USB port requires a cable to connect the dark room
assembly to an external PC for control. Imaging applications
are made easy by a pre-focused camera that requires little or
no manual adjustment, while simple one-click image acquisition and analysis software guides the user through every
step of the gel documentation process. A front LED indicator panel reveals at a glance the light source in use, whereas
a viewing screen with universal filter and spring-loaded
cover facilitates safe and convenient gel inspection.
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OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
• Accessory white light table and blue lights
allow easy switching between ethidium
bromide, safe stained and protein gels
omniDOCi
The omniDOCi shares all of the same features of the
standard omniDOC, but with the added benefit of wireless
connectivity to a remote laptop or tablet. Simply install
the omniDOC image acquisition and analysis software on
a laptop or tablet, place the gel on the transilluminator or
white light table within the darkroom, and then begin image
capture, using your preferred excitation source and filter,
either by ‘pressing’ the tabs on the omniDOC’s front panel
colour touchscreen, or by following the prompts within the
software on your laptop or tablet. Once image acquisition
is complete the gel may be analysed immediately using the
complimentary analysis software included, or saved for later
to perform analysis at a more convenient time and place.
The software is downloadable as an app to iPAD, and Windows and Samsung Android tablets to provide full touchscreen remote control, making the omniDOCi probably the
most portable and versatile imaging system on the market.
Typical Applications
Documentation and analysis of DNA, Safe Stained and
Protein gels
• Dark room assembly with corrosion resistant
ABS construction
• Safety switch – prevents accidental UV
exposure when opening front door panel
• Power on-off switch
• USB port for PC connectivity in omniDOC,
and used for maintenance and installation of
updates in omniDOCi
• Wi-Fi connection for wireless remote control
and image transfer to complimentary
image acquisition and analysis software
downloadable as an App (in omniDOCi
models only) or supplied on disc for
installation onto a laptop or PC (omniDOC
and omniDOCi)
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98
I GEL DOCUMENTATION
GEL DOCUMENTATION I 99
OMNIDOC and OMNIDOC-i Applications
ORDERING INFORMATION
Dot-blots – use
the omniDOC
software to
acquire and
analyse nucleic
acid dot blots
DNA – use the slide-out UV
transilluminator to capture
images of DNA gels stained
with EtBr and SYBR dyes
Blue light – LED epiillumination module allows
visualisation of some stains
with better clarity and without
DNA damage – e.g. runSAFE
White light table – use plugin white light table to view
coomassie blue and silver
stain protein gels; may also be
used to view tissue slides and
autoradiographs
Autoradiographs – high
resolution 5MP camera
captures images in high detail,
especially when scrutinising
separation between closely
located bands or spots
OMNIDOC
Omnidoc Gel Documentation System with 620nm
(EtBr) emission filter & 312nm UV transilluminator*
OMNIDOCSAFE
OMNIDOC plus Blue LED Epi-illumination Module
(OMNIDOC-BL), and 520, 560 & 580nm filters
(OMNIDOC-SYBR, -AF560 & -AF580)*
OMNIDOCPRO
OMNIDOC plus White Light Table (OMNIDOC-WLT)*
OMNIDOCPROSAFE
OMNIDOC plus Blue LED Epi-illumination Module
(OMNIDOC-BL), and 520, 560 & 580nm filters
(OMNIDOC-SYBR, -AF560 & -AF580); and White
Light Table (OMNIDOC-WLT)*
*Requires a PC or laptop with USB cable
OMNIDOCi
Omnidoc-i Gel Documentation System with 620nm
(EtBr) emission filter & 312nm UV transilluminator†
OMNIDOC-i plus Blue LED Epi-illumination Module
OMNIDOCiSAFE (OMNIDOC-BL), and 520, 560 & 580nm filters
(OMNIDOC-SYBR, -AF560 & -AF580)†
Acquire, store and manipulate images
Analyse, document and quantify gels
Use intuitive touchscreen control for image acquisition in a few simple
steps
Following image acquisition use the intuitive touchscreen control software for analysis
Adjust the exposure time, altering the UV intensity or manipulating the
iris on the camera if required
Load the newly acquired image, or select one stored previously in TIFF,
JPEG, BMP or GIF image format
Select your light source: UV, blue or white light
Select the gel or dot blot type from one of four options
Use Toolbox function to change default settings for excitation source
& exposure time, or apply advanced features like saturation detection &
date stamp
‘Tap and drag’ rectangular boxes on your tablet to define the sample
lanes to be analysed
Image Freeze – minimise UV damage nucleic acid gels by ‘freezing’ the
gel image and switching off the transilluminator ahead of image capture
or printing
Set the level of sensitivity and define the base line for subtraction
Acquire and save image for…
Perform density analysis…
Analysis.
And then molecular weight analysis; use software to save as an image
file format of your choosing or export into Microsoft Excel as a CSV file
for further data analysis
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
OMNIDOC-i
312nm, 21x26cm (WxL); 6x8W tubes
Resolution
5 mega pixels (2592x1944 pixels maximum)
Sensor
CMOS, 1/2.5”
Lens
5mm focal length; aperture F1.2
Image Bit-Depth Sensor
12-bit (0-4095 grey levels)
Filter Camera
620nm EtBr (standard); optional 520, 560,
580nm filters
PC or Laptop
Laptop, PC or iPAD,
and Windows® and
Samsung Android
tablets
USB to PC
Wi-Fi to PC or tablet
Image Storage
Connection to Operating Device
Operating System Requirements for Software
Dark Room Assembly
Dimensions
Windows® 7 (64bit & 32bit) / XP / Vista
410 x 405 x 570mm (W x D x H)
OMNIDOC-i plus White Light Table (OMNIDOC-WLT)†
OMNIDOCiPROSAFE
OMNIDOC-i plus Blue LED Epi-illumination Module
(OMNIDOC-BL), and 520, 560 & 580nm filters
(OMNIDOC-SYBR, -AF560 & -AF580); and White
Light Table (OMNIDOC-WLT)†
Front Panel Display
†Requires a PC, laptop or tablet with Wi-Fi connection
White Light
6x1W LED (standard) for gel positioning
White Light Table
(optional)
Blue LED Epi-illumination Module (optional)
21x26cm filter; connects internally to dark
room
OMNIDOC-WLT Optional White Light Table
Use the complimentary omniDOC software supplied to…
OMNIDOC
UV Transilluminator
OMNIDOCiPRO
ACCESSORIES for OMNIDOC & OMNIDOC-i
OMNIDOC Image Capture and Analysis Software
Technical Specifications
OMNIDOC-BL
Blue LED Epi-illumination Module
OMNIDOC-EB
Replacement EtBr filter, 620nm (supplied as
standard)
OMNIDOC-SYBR
YBR Filter (520nm)
OMNIDOC-AF580
Orange Filter (580nm)
OMNIDOC-AF560
Yellow Filter (560nm)
OMNIDOC-F1
Replacement viewing window, Yellow Filter,
560nm (supplied as standard)
Viewing Window
Safety
USB Port
LED
560nm universal orange filter
excitation wavelength 470nm; connects
internally to dark room
Safety interlock switch on front door panel;
disconnects UV transilluminator on opening;
complies with CE, FCC standards
For PC connection For updates and maintenance
-
Wi-Fi Format
Dual voltage: 110-230VAC
Weight
25kg
Filter
• High resolution 5MP
camera with CMOS
sensor for enhanced
light sensitivity
Filter slide with four
interchangeable
filter options:
• Filter slide with four
filter options to
perform an extensive range of
fluorescence
applications
Wireless N, wireless G
Power Rating
Camera
• Lens: focal length
6mm; aperture size
F1.2; autofocusing
3.5” 64K colour TFT
display; touchscreen
• 620nm filter
(standard) – EtBr,
Gel Red & SafeView Classic
• 520nm filter (SYBR) – Gel Green, Midori Green, runSAFE, SYBR Green I & II, SYBR Gold & SYBR Safe
• 560nm filter (yellow) – as per 520nm filter but also
including SYPRO Orange
• 580nm filter (orange) – EtBr, Gel Green & Red, SafeView Classic; SYBR Green I & II, SYBR Gold & Safe;
SYPRO Orange & Ruby
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100
I GEL DOCUMENTATION
GEL DOCUMENTATION I 101
Uvitec Gel
Documentation
Systems
Cleaver Scientific is delighted to announce a privileged
UK partnership with Uvitec Ltd. Based in Cambridge,
Uvitec has served the molecular biology market since
1996 by manufacturing high quality fluorescence and
chemiluminescence imaging systems, and analysis software.
A synopsis of the Uvitec range is offered below .Products
may be supplied either as standalone fully integrated systems
to address all imaging applications or customised to meet
specific individual application and budgetary requirements.
For further information, or to receive a Uvitec catalogue or a
quotation, please do not hesitate to contact us.
• BENEFITS
INCLUDE
• Nearly 20 years’ experience in the
manufacture of high quality imaging systems
and software
• Total versatility: systems may be supplied as
fully-integrated imaging solutions or to suit
any budget or application
• Full technical support and comprehensive
warranty
features:
UVITEC PRODUCT SYNOPSIS
Product Category
Application
Solution
Advanced Systems –
Chemiluminescence &
Fluorescence Imaging
Chemiluminescence & multi-wavelength
fluorescence imaging
ALLIANCE CHROMA
Complete chemiluminescence & UV
fluorescence imaging
ALLIANCE 7
Chemiluminscence & optional UV fluores- ALLIANCE LD
cence imaging
Gel Documentation –
Fluorescence & Colorimetric
samples
TLC & UV Instruments –
Sophisticated lighting
solutions
• Programmable microprocessor control
• Automatic monitoring of UV energy
• Small footprint with large interior
Dedicated low-cost chemiluminescence
ALLIANCE MINI
Highest specification PC-controlled CCD
fluorescence imaging
PLATINUM HD7
• Large visible front panel LED, with non-UV
transmissible front door connected to safety
interlock switches
PC-controlled gel documentation and
quantification
FIREREADER V4
• Observation window – UV blocking
Low-cost PC-controlled rapid image
capture and printing
ESSENTIAL V4
• Membrane keypad operation
Standalone fluorescence and colorimetric
gel documentation; no PC required
UVIDOC HD5
Basic low-cost gel imaging; no PC required; suitable for education
UVISAVE HD5
Dedicated PC-controlled TLC imaging
system
FIREREADER TLC
UV filtered lamps for fluorescence and
TLC techniques
UVILITE
Viewing cabinets for fluorescent samples
and TLC plates
UVICAB
Enhanced UV transillumination & documentation free of unwanted background
fluorescence
UVIPURE
Standard UV transillumination
Germicidal UV for Sterilisation
Replacement UV tubes
Typical Applications
Chemiluminescence and Multi-Wavelength Fluorescence.
Colorimetric imaging. Thin Layer Chromatography plates
ORDERING INFORMATION
CL-508.G
Shortwave crosslinker, 254nm
CL-508.M
Midrange crosslinker, 302nm
CL-508.BL
Longwave crosslinker, 365nm
TECHNICAL SPECIFICATION
UV Source
5x8W UV Bulbs, 254, 302 or 365nm
Exposure Time
0 – 999.9 minutes
Energy Ranges
0 – 99.99 J or 0 – 9.999 J
Internal Dimensions
26x33x14.5cm (w x d x h)
UVIVUE
Exterior Dimensions
35x35x30cm (w x d x h)
UVILITE GERMICIDE
Weight
10.5Kg
UVITUBE
Footprint
35x36cm
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FOR MORE INFORMATION
Typical Applications
Crosslinking DNA and binding to membranes. Colony and
plaque studies, UV sterilisation and gene mapping.
UV Crosslinker
The UVIlink UV crosslinker is especially designed for binding
nucleic acids to membranes. A membrane keypad facilitates
manual or preset control of the desired UV dosage and
exposure time, while a highly accurate microprocessor-controlled photo-feedback system maintains uniform output
from each of the crosslinker’s five 8-Watt UV bulbs. Other
features comprise safety interlock switches to prevent accidental UV leakage during operation, a clearly visible LED,
plus a large interior chamber and small footprint area. The
crosslinker may be used in a variety of applications, such as
colony or plaque lifts, UV sterilisation and gene mapping or
DNA damage studies.
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102
I GEL DOCUMENTATION
UV
Transilluminators
Available in single and dual wavelength formats, in 21x21cm
and 21x26cm sizes, our transilluminators are supplied either
as standalone units or with the microDOC™, as part of a
fully integrated gel documentation system. With a large
surface area, each transilluminator serves as the perfect
workstation for viewing and working with fluorescentlystained nucleic acid and protein gels.
Standard features include a high/low intensity safety switch
and an efficient starter that allows each of the six 8-Watt
UV tubes to energise quickly without flickering, while special
filter glass minimises unwanted background light. All of
these features maximise contrast and sensitivity, allowing
even the faintest fluorescent gels to be viewed. Two dual
wavelength models offer added flexibility and convenience.
TECHNICAL SPECIFICATION
Filter Size
21 x 21 cm or 21 x 26cm
Light Source
8W x 6 tubes
Intensity Switch
High (100%) / Low (70%) intensity
switch for single wavelength mode
Fast Start Up
New high quality starter to
simultaneously illuminate tubes when
switched on with no blink
UV resistant plastic cover
33 x 25 cm (w x d )
Unit dimensions (w x l x h)
34 x 29.5 x 10 cm
Typical Applications
Illumination of stained gels for analysis, band cutting and
documentation.
GEL DOCUMENTATION I 103
safeVIEW
features:
Our new safeVIEW LED transilluminator offers the user a
safe way to view and document their samples.
• Three wavelength options: 254/312/365 nm
– Two dual wavelength models
features:
• Long life filter
• No DNA damage to samples.
• High efficiency reflector
• Safer for user - No UV light.
• Hi/Lo intensity switch
• High Purity LED light.
• Fast start up
• Strong Metal enclosure with stainless steel
filter frame.
• Fast start up.
ORDERING INFORMATION
CSLUVTS254
UV Transilluminator, small, 21 x 21 cm, 254 nm
CSLUVTS312
UV Transilluminator, small, 21 x 21 cm, 312 nm
CSLUVTS365
UV Transilluminator, small, 21 x 21 cm, 365 nm
CSLUVTSDUO
UV Transilluminator, small, 21 x 21 cm, 254/365 nm
BLUE Light Transilluminator 21 x 21cm
Accessories
CSLUVTSDUO365L UV transilluminator, small, 21 x 21 cm, 312/365 nm
CSL-UVPS22
UV Transparent Cutting Platform 22 x 22cm
CSLUVTS254L
UV Transilluminator, large, 21 x 26 cm, 254 nm
CSL-UVPS27
UV Transparent Cutting Platform 22 x 27cm
CSLUVTS312L
UV Transilluminator, large, 21 x 26 cm, 312 nm
CSLUVTS365L
UV Transilluminator, large, 21 x 26 cm, 365 nm
CSLUVTSDUOL
UV Transilluminator, small, 21 x 26 cm, 254/365 nm
TECHNICAL SPECIFICATION
Filter Size
21 x 21cm
Replacement Parts and Accessories
Light Source
470nm BLUE LED’s
CSL-Txxx
8W UV bulb (xxx = 254 nm, 312 nm or 365 nm)
Size
340 x 270 x 130mm
CSL-UVPS22
UV Transparent Cutting Platform 22 x 22 cm
Weight
5Kg
CSL-UVPS27
UV Transparent Cutting Platform 22 x 27 cm
Voltage
110- 240V (Selectable)
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OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
They are supplied as a standalone unit and can be used
with the microDOC™, as part of a fully integrated gel
documentation system. With a large surface area, each
transilluminator serves as the perfect workstation for viewing
and working with fluorescently-stained protein and nucleic
acid gels.
ORDERING INFORMATION
Safeview
CSLUVTSDUO312 UV Transilluminator, small, 21 x 21 cm, 254/312 nm
CSLUVTSDUO312L UV Transilluminator, small, 21 x 26 cm, 254/312 nm
This light source also has the added advantage that it does
not cause damage to dna or rna that would normally be
associated with uv light.
Typical Applications
A safe way for the operator to view their samples with no
dna or rna damage.
High purity led light.
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104
I GEL DOCUMENTATION
GEL DOCUMENTATION I 105
features:
• For scanning of colorimetric protein gels,
blots and TLC plates
• Sealed unit suitable for wet gels
• User has full control over resolution,
compression and scan speed, providing
complete flexibility in choosing between high
speed or high quality image acquisition
• Transmittance and reflectance modes for
different imaging applications, whereas
colour channel separation allows accurate
detection of all colorimetric dyes
duoVIEW
Our new duoVIEW transilluminator offers the user the
added advantage of being able to view their gels with either
standard UV light or using the safer blue light technology.
This means only one transilluminator is needed for ethidum
bromide and safe dye stained gels. They can either be used
as a standalone unit or with the microDOC™, as part of
a fully integrated gel documentation system. With a large
surface area, each Duoview serves as the perfect workstation
for viewing and working with Nucleic acid and fluorescently
stained protein gels.
• Create and store acquisition methods for
different applications to ensure scan-to-scan
reproducibility; greyscale wedge for system
calibration and calibration reporting
• Ideal for post-electrophoresis scanning of
omniPAGE Mini, Midi, Maxi and WAVE gels
features:
• UV & Blue light technology in one
transilluminator.
• Single or dual wavelength models avaialble.
• Industry leading long life UV filter
ORDERING INFORMATION
CSL-MSCAN
microSCAN complete documentation
system for 1-D and 2-D colorimetric gels –
240VAC*
CSL-MSCAN$
microSCAN complete documentation
system for 1-D and 2-D colorimetric gels –
110VAC*
• Fast start up
*Each system includes Epson scanner with transparency unit; power
supply and USB cable; control software and greyscale wedge for
system calibration
ORDERING INFORMATION
TECHNICAL SPECIFICATION
Filter Size
Technical Specifications
DUOVIEW254
UV254 & BLUE Light Transilluminator 21 x
21cm (110V-240V)
System
High Quality 1-D and 2-D A4 scanner with
transmittance and reflectance modes
DUOVIEW312
UV312 & BLUE Light Transilluminator 21 x
21cm (110V-240V)
Max. Gel Imaging Size
20.9x28.5cm (reflectance)
19.7x23.7cm (transmittance)
DUOVIEW365
UV365 & BLUE Light Transilluminator 21 x
21cm (110V-240V)
Optical Resolution
2400dpi
Resolution
16-bit greyscale; 48-bit colour
Scan Optical Density
Range
3.8 OD
Spectral Range
430-745nm
Interface
USB 2.0, Firewire
Dimensions (w x d x h)
30.8x53x15.3cm
21 x 21cm
DUOVIEW254/312
UV254/312 & BLUE Light Transilluminator
21 x 21cm (110V-240V)
DUOVIEW254/365
UV254/365 & BLUE Light Transilluminator
21 x 21cm (110V-240V)
Light Source
470nm BLUE LED’s or UV Single Wave (8W
x 5 tubes) or UV dual Wave (8W x 9 Tubes)
Intensity Switch
High (100%)/ Low (70%) Single
Size
400 x 190 x 350mm
Accessories
Weight
10.5Kg
CSL-UVPS22
UV Transparent Cutting Platform 22 x 22cm
Weight
6.6Kg
Voltage
110- 240V (Selectable)
CSL-UVPS27
UV Transparent Cutting Platform 22 x 27cm
Power
110-240VAC
Typical Applications
Viewing of DNA & Protein Gels
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Typical Applications
Scanning and documentation of gels and blots.
microSCAN
2-D and 1-D A4 Gel
Scanner
The microSCAN is a low-cost documentation system for
scanning colorimetric 1-D and 2-D gels, as well as blots
and TLC plates. The microSCAN’s control software allows
rapid generation of high quality images that may then be
imported into recommended TotalLab Quant and Phoretix
1D software (Pg 107) for analysis. Users may choose
between transmittance and reflectance modes in colour
and greyscale, and can select their required resolution.
The microSCAN can generate high resolution images at
2400 dpi, while its variable colour scanning functionality is
sensitive enough to detect the faintest protein spots in gels
stained with Coomassie Blue, silver and other colorimetric
stains. As a sealed unit with a maximum 20.9x28.5cm
scanning area, the microSCAN is also suitable for wet protein
gels, particularly those run in the omniPAGE Mini and Midi,
and Maxi and WAVE vertical systems (Pg 51).
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106
I GEL DOCUMENTATION
GEL DOCUMENTATION I 107
ORDERING INFORMATION
TotalLab 1D
TotalLab 1D analysis module for agarose and
PAGE gels; available only with the purchase of
MicroDOC1D models (Pg 95)
TotalLab Quant
TotalLab 1D analysis module plus array analysis,
colony counting & toolbox modules
Phoretix 1D
Phoretix 1D software for gel quantitation,
calibration and band pattern matching studies
within a single gel
Phoretix 1D Pro
Phoretix 1D software for gel quantitation,
calibration and band pattern matching studies
between multiple gels
Gel Analysis Software
TotalLab and Phoretix
TotalLab and Phoretix gel analysis
software options are available for
quantitative gel analysis following gel
documentation. Each software option
offers the highest level of automation
currently available and guides the
user step by step through the analysis
process. A user-friendly interface is
split into four parts allowing the user to
view within a single screen every aspect
of gel quantitation, including the gel
image, lane and band profiles, analysis
data and the help menu.
TECHNICAL SPECIFICATION
TotalLab TotaLlab Phoretix Phoretix
1D
Quant
1D
1D Pro
Compatible with 21CFR part
11 compliance module
✓
✓
✓
Automatic detection of lanes
and bands
✓
✓
✓
✓
Automatic background
subtraction
✓
✓
✓
✓
Image manipulation tools
✓
✓
✓
✓
Lane templates
✓
✓
✓
✓
✓
✓
Analysis protocols for batch
processing
Molecular weight calibration
✓
✓
✓
✓
Quantity calibration &
normalisation
✓
✓
✓
✓
Profile deconvolution
✓
✓
✓
✓
Rf calibration
✓
✓
Band picking
✓
✓
Band pattern matching –
single gel
✓
✓
Band pattern matching –
lanes across multiple gels
✓
Band pattern queries
✓
Dendrogram – single gel
✓
✓
Data archive and search
facility
✓
Classification and
identification tools
✓
✓
TotalLab 1D Module which is
✓
Dendrogram – lanes from
multiple gels
Reports
TotalLab is used primarily for 1D gel
quantitation and is suitable for all users
regardless of their experience. More
advanced Phoretix software, which
also includes a free copy of TotalLab
Quant, is recommended for laboratories
performing in-depth lane relationship
studies. Details of each software option
are as follows:
✓
✓
✓
Array analysis module
✓
✓
✓
Colony counting module
✓
✓
✓
Toolbox for general analysis
✓
✓
✓
the 1D gel analysis module of TotalLab
Quant, is the software supplied
exclusively with all microDOC1D
models (Pg 95). TotalLab 1D features a
user-friendly interface and help menu
that provide a simple, guided workflow
for fast and accurate quantitation and
calibration of 1D gels and western blots.
Main benefits include:
• The capacity to review each step
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within the automated workflow
analysis, and manually intervene or
edit if desired
• Highly developed algorithms which
accurately detect lanes and bands
even on distorted gel images
• A range of visualisation tools that
facilitate further examination of
lane and band data to verify results,
including band calibration from
Molecular Size standard lanes and
accurate quantitation derived from
known band volumes
• Full user control over the visualisation
tools and the data displayed,
allowing only the important data
fields and images of choice to be
selected for final output
• Multiplex analysis
TotalLab Quant includes
TotalLab1D plus three modules for array
analysis; colony counting and 2D spot
measurement; and general featurebased image analysis. The array analysis
module can automatically detect up
to 1536 wells or arrays spots, and
may also be used to quantify dot and
slot blots. Array analysis and Toolbox
modules also include multiplex analysis
functionalities.
Phoretix 1D is more advanced
analysis software used primarily for
band-pattern matching within individual
Typical Applications
Analysis of gel bands and 1D quantification.
DGGE, SSCP and RFLP gels that are
important for cultivar experiments,
evolutionary biology and population
genetics. Phoretix 1D has a powerful
band matching feature, which is
flexible and easy to use, while visual
tools show the results of matching
and identify similarities within an
individual gel, including lane clustering
via dendrograms. A copy of TotalLab
Quant is included with Phoretix 1D.
Phoretix 1D Pro shares the same
analysis features as Phoretix 1D, but
with added database storage to provide
the ideal solution for generating and
manipulating large lane-comparison
datasets from multiple gels. Benefits
include:
• Long-term archiving and storage of
data from multiple gels
• Comparison of each lane with any
other lane within the database, with
the results presented as dendrograms
or tables that show all band and
lane similarities, thereby enabling
identification of lane relationships
across many different experiments
• Classification and identification of
unknown sample against a defined
library of known references that
can be easily shared between
co-workers and labs making
collaboration on large projects easier
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108
I GEL DOCUMENTATION
GEL DOCUMENTATION I 109
Statistics
Experiment Design Setup
Image Alignment
• 100% matched data enables you to
apply robust and reliable multivariate
statistics including, False
• C
hoice of experiment design setups
– either “between-subject design” or
“within-subject design” (e.g. same
patients sampled before, during and
after treatment)
• A
utomatically align images at the
pixel level before image analysis
• Discovery Rate (q-values), Principal
Components Analysis, Correlation
Analysis and Power Analysis
• Highlight the interesting spots on a
whole gel image view
• Import findings from any other
statistical analysis packages and link
them to spots in your experiment
View Results
• Differentially expressed spots are
automatically sorted by significance
based on ANOVA p-value
• V
alidate expression changes using 2D
and 3D montage views, expression
profiles and spot data tables
• Perform any editing on a single
image and apply the change to all
other images automatically
• Quickly create populations of
spots with shared, user-defined
characteristics using tags
• Display a CV plot to check
experimental variation is within the
limits defined by your lab
• Interesting spots are taken forward
for statistical analysis, picking and
reporting
• S et up multiple comparisons of data
within the same experiment
SameSpots analysis
• A
utomatic analysis of the aligned
image set and correction of dye
related offsets in DIGE
• T
he result is a common spot outline
map across every image with 100%
matching and no missing values for
robust statistical analysis
• U
nique normalisation method that is
unaffected by outliers and saturated
large spots
Filtering & Normalisation
Review
• E xclude any artefacts and/or damaged
areas of your gel from the analysis
• R
eview results of normalisation and
take corrective action if required to
maintain experiment quality
ORDERING
INFORMATION
Progenesis2D
Software for 2D gel analysis
Image QC
Image Alignment
Multivariate Statistics
Spot Picking
View Results
• A
ccuracy can be assessed before
proceeding using a simple visual
application
• P
ixel-perfect alignment makes it
possible to generate a common spot
outline map across every image
• M
ake detection applicable to your
experiment by selecting the images
the software uses to generate the
• S ameSpots outline map
Image Quality Control
• A
utomatic assessment at the start
of your workflow highlights many
common problems with image
quality before you proceed to the
next step. Tools are included to crop,
flip, rotate and invert images.
• Image intensity histogram shows
intensity levels and dynamic range of
your captured gel images
Image Import
• A
nalyse single stain, 2D DIGE and
multiple-staining of the same gel.
Supports .tiff, .img, .gel, .mel images
• E xtend your analysis at any time
by adding new images to existing
experiments
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Reproducible 2D gel analysis
is possible Be confident in your proteomics data...
Progenesis SameSpots from Total Labs is the 2D gel analysis software of choice for reproducible proteomics results. It
enhances your analysis workflow with speed, objectivity and statistical power. You also have features to perform detailed
investigations of your data, support spot picking and generate custom reports.
Speed
Progenesis SameSpots
Typical analysis times of 5mins/image*
with a fast, streamlined workflow.
Faster analysis times means you can run
more replicates.
Key Steps in the Workflow:
Objectivity
Easy-to-use and proven to give highly
reproducible results across-labs. Post
analysis editing is eliminated on the
majority of experiments.
Statistics
Increased statistical power with 100%
matching in your experiment and no
missing values, so you can apply valid
multivariate statistics.
• Q
uickly share a report of protein
spots defined as significant,
interesting or those you selected for
picking
• P
rint or save the report. Images and
data can be cut and pasted into other
applications
Spot Calibration & Picking
• P
ick spots using a unique image
alignment approach for high
precision
• S upports common spot cutting
robots including GelPix, ProPic™
and Ettan™ as well as manual
picking
• p
I and Molecular Weight calibration
GENERAL LAB EQUIPMENT – OVERVIEW
GENERAL
LAB
EQUIPMENT
Please see below a synopsis of the general laboratory equipment now available through Cleaver Scientific.
NEW PRODUCTS
GTC96S gradient thermal cycler delivers affordable, high performance PCR of DNA in 96-well plates and 0.2ml tubes and strips.
RMC24 refrigerated micro centrifuge sets a new standard in laboratory bench top centrifugation for molecular biology
applications. Equipped with high speed 24-place rotor for 24 × 1.5/2.0 ml tubes, 2 × PCR strips or 16x0.2ml tubes.
LIQUID HANDLING
omniPETTE – ergonomically designed single and multichannel pipettes provide accurate and precise liquid handling. Left- and right-handed operation,
fully autoclaveable and UV resistant.
omniPET pipette filler – cordless, rechargeable and with a digital LCD to show volume – dispense 0.5-100ml volumes.
Pipette tips – available on request – please enquire.
CENTRIFUGES, VORTEX AND SHAKER
Quickspin microcentrifuge for rapid spin-downs of samples in 1.5/2ml microtubes and 0.2ml strips.
multiFUGE – includes a unique duoROTOR for simultaneous centrifugation of 12x 1.5/2ml microtubes and 32x 0.2ml PCR tubes.
Vortex – combines fast, efficient mixing with minimal vibration. Optional head attachments for 0.2ml tubes and strips, 1.5/2ml tubes and larger 15ml
and 50ml tubes.
MiniMix 3D Shaker combines orbital shaking with rocking to provide the perfect speed and tilt for blotting incubations and gel staining. A range of blot
boxes available in different sizes.
DRY BATHS, HEATERS AND STIRRERS, STIRRING WATER BATHS
microBLOCK – personal, ultra-compact mini dry bath for tubes ranging from 0.2 to 50ml in size.
Cube Dry Baths – with digital microprocessor controller for accurate temperature control in 0.1°C increments from ambient +5°C to 150°C, for applications including restriction digestion and Hot Start PCR. Single and dual block models with interchangeable blocks to accommodate different tube
volumes.
Stirring water baths with either 1 or 3 built-in stirrers in 10 and 20L volumes.
PERISTALTIC PUMPS
Supplied in single, dual and tetrad pump-head formats with a selection of silicone tubes. Ideal for buffer recirculation and gradient gel formation.
HYBRIDISATION OVENS
Used in numerous temperature sensitive applications, such as enzyme assays, nucleic acid probe-hybridisations and overnight cultures. The entry-level
hybridBASIC includes 2 stainless steel shelves, while more sophisticated models include vortex, orbital, reciprocal and rocking shaking-platform formats
with optional tube rotisseries, double-capacity platform, and 250ml and 500ml flask holders.
UV STERILISATION, COOLCUBE AND ICE BUCKETS
CSL-UVCAB and CSL-UVCABMINI – serve as low cost alternatives to clean rooms to provide a convenient area to set up PCR reactions in an environment free from background nucleic acid contamination.
Consort pH meters – measure pH, conductivity, total dissolved salt, salinity and temperature, and may be supplied with electrodes, buffers and standards.
CoolCube – allows temperature-sensitive samples to be retained on the bench at 4°C for up to 4 hours.
Ice buckets made from durable plastic and supplied with lids to keep samples at 0°C.
Key Features:
• A full array of products
for electrophoresis-related
applications
• Include: pipettes for liquid
handling; heating blocks
and dry baths for enzyme
incubations and sample
denaturation; heaters and
stirrers, and stirring water
baths to dissolve solids
in buffers and samples;
peristaltic pumps for
gradient gel formation;
hybridisation incubators
for northern and Southern
blotting applications; UV
cabinets and gradient
thermal cyclers for PCR;
pH meters; ice buckets for
storage of temperaturesensitive samples;
recirculating chillers for
active gel temperature
control; refrigerated
centrifuges to harvest living
cells; gel dryers and vacuum
pumps; rockers and shakers;
glove boxes and radiation
safety.
• Guaranteed minimum
1-year warranty
CHILLER, GEL DRYERS AND VACUUM PUMPS
CSL-CHILLER – recirculating chiller ready to use with insulated tubing and clips, and a 3-year warranty. The preferred cooling option for the VS20WAVE electrophoresis and blotting units.
ROCKERS AND SHAKERS
Four models in orbital, reciprocal, rocking and 2-D/3-D gyratory shaking-platform formats. Additional platforms double capacity without increasing
footprint area. Optional water bath for nucleic acid hydridisations.
All laboratory needs are met.
Regardless of whether it’s a microcentrifuge to spin-down
protein samples denatured in a heating block ahead of gel
loading, a thermal cycler, or a hybridisation incubator and
shielding for manipulation of radioactively labelled nucleic
acid probes, The range of Cleaver Scientific laboratory
products offers a solution.
Pipettes112-113
Centrifuges114-117
Vortex and MiniMix 3D Shaker
118-119
Dry Baths and Thermal Cycler
120-123
Heaters and Stirrers
124
Stirring Water Baths and Peristaltic Pumps
125
Hybridisation Incubators
126-127
UV Cabinets with option of HEPA Filter
128
pH Meters and Ice Buckets
129
Chiller, Gel Dryer and Pump
130-131
Rockers and Shakers
132-133
RELATED PRODUCTS
Gel dryers supplied in 21x31cm and 35x45cm sizes.
Vacuubrand MZ 2C + 2 AK – the preferred vacuum pump option for Cleaver Scientific gel dryers.
Cleaver Scientific offers a comprehensive collection of
laboratory equipment for molecular biology and life science
laboratories
Balances page 153
Radiation protection
products
page 148
Reagents and
chemicals
page 134
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112
I GENERAL LAB EQUIPMENT
GENERAL LAB EQUIPMENT I 113
Multichannel Manifold
omniPETTE multichannels feature a revolutionary suspension
system which allows the shafts to retract slightly when they
are pressed against a row of pipette tips. Each shaft moves
independently, resulting in an even sealing force being applied
to all 8 or 12 tips. This ensures that all tips secured on their
individual shaft with the minimum of effort - and never fall off!
The suspension system not only ensures secure tip sealing but
also eliminates the common incidence of tips being jammed
on pipette outer shafts. The innovative ejection bar is curved,
allowing the tips to be pushed off in steps, therefore reducing
the amount of force required for ejection. For comfortable
pipetting in any direction improved omniPETTE manifold now
ejects tips within 360o.
UV Resistant
omniPETTE
Variable Volume Single and Multichannel Pipette
Ergonomically designed omniPETTE single and multichannel
pipettes feature slim handle, high accuracy and precision
rates combined with their tremendous robustness and very
competitive price.
Safety & Comfort
Distinctive handle of these pipettes with ejector pushbutton
located on the top of the finger rest and the volume counter
facing backwards offer their users the sense of comfort and
continuous control of the pipetting volume. omniPETTE single
channel pipettes feature PVDF shafts ensuring increased
chemical and mechanical durability of the product.
omniPETTE pipettes are available in wide range of volumes:
from 0.1 µl to 10000 µl.
Large Volume Display
Easy-to-read numbers
Accuracy and Precision
High technology design, and sophisticated space age materials
are used for our pipettes production. Each pipette is individually
tested (Quality Control Certificate included) on the most
precise electronic balances.
Crafted out of highly durable materials omniPETTE Series
Pipettes can be safely sterilized with UV radiation. Even
prolonged exposure to UV beams does not damage the plastic
and the de-coloration of the pipette parts is minimal.
Recalibration System
Calibration key provided with omniPETTE allows for quick and
accurate pipette recalibration.
Reliable Pipette – Tip System
Cleaver Scientific offers the wide range of pipette models.
Shafts of omniPETTE are designed to fit all popular tips. To
ensure outstanding precision and repeatability each Cleaver
Scientific pipette is manually calibrated. The performance of
every pipette is checked by gravimetric method and the results
of test are printed in pipette Quality Control Certificate.
ORDERING INFORMATION
omniPETTE Single Channel
Volume µl
Volume µl
CV2
0.2 - 2ul
CV200
20 - 200ul
CV10
0.5 - 10ul
CV1000
100 - 1000ul
CV20
2 - 20ul
CV5000
1000 - 5000ul
CV100
10 - 100ul
CV10000
1000 - 10,000ul
Volume µl
omniPET is a revolutionary engine powered pipette filler
with LCD display designed for cordless work with glass or
plastic pipettes in the 0.5-100 ml range. While designing
the omniPET we made users convenience a priority.
Carefully modeled lightweight handle, together with
smooth pushbuttons and switches located “right where
they are needed” guarantee effortless pipetting even during
extensive use. For convenient storage our tool is equipped
with “rest wings”. LCD display indicates setting options and
level of battery charge.
Adjust It To Suit Your Work
Easily accessible switches of omniPET allow choosing
different operation mode depending on the volume of
pipette and viscosity of liquid. For liquid aspiration the user
can choose HIGH or LOW work speed and additionally
adjust the suction by the pressure applied to the trigger
button. Dispensing can be carried out by gravity (GRAV) or
supported by pump (BLOW) which empties the pipette with
blow out. The settings are shown on the LCD display.
ORDERING INFORMATION
Volume µl
CV8-10
0.5 - 10ul
CV8-200
20 - 200ul
CV8-50
5 - 50ul
CV8-300
50 - 300ul
Pipette filler
Omnipet
omniPETTE -Multichannel
Volume µl
Volume µl
CV12-10
0.5 - 10ul
CV12-200
20 - 200ul
CV12-50
5 - 50ul
CV12-300
50 - 300ul
New pipetting mechanism allows for precise and effortless
setting of pipette volume. Winding the counter from min to
max volume can be performed with one hand in minimal time.
Pipettes Stands
Measuring and dispensing of low to medium sample
volumes
Pipetting Never Felt So Good
omniPETTE 8-Multichannel
Smooth Adjustment of Pipette Volume
Typical Applications
omniPET Pipette
CV-RS
Rotating Stand
CV-4POS
CV-1POS
1 Position Stand CV-MS
4 Position Stand
Multiple Stand
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omnipet, with charging stand
Technical Specifications
Autoclavability
Nosepiece, pipette holder, filter
Filter
Hydrophobic PTFE 0,2µm
Pipette types
Glass or Plastic 0.5-100ml
Typical Applications
Measuring and dispensing of medium to large sample
volumes
No Worries About Safety
Many pipette fillers are not sufficiently protected against
overfilling. When an aggressive liquid enters the body of a
pipetting aid it may damage the tool or constitute a threat to
the users health. Therefore we have equipped the omniPET
not only with standard PTFE filters, but also a safety valve,
blocking any liquid from entering the unit. To protect your
samples from cross contamination, filters and pipette holders
can be easily exchanged and autoclaved.
• Suitable for 0.5 ml to 100 ml pipettes
• Ergonomically shaped handle
• LCD display
• Sensitive valves for precise work with low volume pipettes
• Low battery light indicator
• Protected by filter and safety valve
• Autoclavable nosepiece and pipette holder
• Charging stand
Always Ready for Action
Powerful, environmentally friendly Ni-MH battery enables
many hours of continuous work. The LCD display indicates
when omniPET should be recharged. You do not have to
stop your work. Just put the power supply jack in and load
your tool while working! omniPET battery is automatically
protected against overcharging by timing and thermal
systems so you do not need to worry about your tool while
leaving it for overnight charge. The omniPET is supplied with
comfortable charging stand.
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114
I GENERAL LAB EQUIPMENT
GENERAL LAB EQUIPMENT I 115
features:
• Supplied with both standard microtube and
strip tube rotors
• Ideal for quick spin-downs and microfiltration
• Starts and stops in seconds
• Compact design
ORDERING INFORMATION
CSLQSPIN
Spectrafuge Mini complete with 1.5/2.0 ml rotor,
strip tube rotor, 0.5 and 0.4ml adapters, purple lid
CSLQSPIN$
Spectrafuge Mini complete with 1.5/2.0 ml rotor,
strip tube rotor, 0.5 and 0.4ml adapters, purple lid
110v
features:
• duoROTOR for microtubes and PCR strips/
tubes
• Twice the capacity of traditional mini
centrifuges
Quickspin
• Near silent operation
• Starts & stops with opening/closing of lid
The Quickspin ‘Purple’ is perfect for microfiltration and rapid
spin-down of sample from the walls and caps of microcentrifuge tubes.
ORDERING INFORMATION
By occupying less than 6 inches square of bench space, the
Quickspin ‘Purple’ has a very small footprint, making it easy
to use in the lab. Rotors and adaptors, which may be easily
interchanged, are supplied as standard to accommodate
1.5 ml, 0.5 ml and 0.4 ml tubes, as well as 0.2 ml strips and
individual tubes.
A highly durable stainless steel hinge pin facilitates easy
opening of the translucent lid, while an on/off switch is
located on the side of the centrifuge to start and stop operation. Alternatively, with the switch in the ‘on’ position,
the centrifuge can be started and stopped by closing and
opening the lid.
Typical Applications
Spinning down (centrifugation) of liquid samples in small
tubes
TECHNICAL SPECIFICATIONS
CSL-MULTIFUGE
multiFUGE Mini Centrifuge, with duoROTOR for
microtubes and PCR strips/tubes
MF-A0.5-6
Adapters, 0.5ml, pack of 6
MF-A0.2-6
Adapters, 0.2ml, pack of 6
TECHNICAL SPECIFICATIONS
Speed
5,500rpm / 2,000xg
2,000 x g
Capacity
6 x 1.5/2.0 ml
2 x 0.2ml Strips
12 x 1.5 / 2.0ml tubes
32 x0.2ml PCR tubes
4 x PCR strips (8x0.2ml)
Maximum speed
6000rpm
Maximum G Force
Capacity
Dimensions
14 x 20 x 11.2 cm
Unit Dimensions (w x l x h) 15 x 15 x 11.7 cm
Weight
1.1 kg
Weight
Electrical
100-240V, 50-60Hz
0.45 kg
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Typical Applications
Spinning down (centrifugation) of liquid samples in small
tubes
CSL Multifuge
Unlike traditional mini centrifuges, the new multiFUGE
Mini Centrifuge eliminates the need to change rotors when
switching between microtubes and PCR strips. Included, the
unique duoROTOR is all that is required for running
12 microtubes and 4 PCR strips simultaneously.
With a fixed speed that produces 2,000 xg, this centrifuge
is perfect for quick spin downs. Simply close the lid and the
unit quickly ramps up to 5500 rpm. Open the lid and the
rotor quickly decelerates for removal of samples.
At just 14 cm wide and 11 cm high, the multiFUGE Mini
Centrifuge trully is a personal centrifuge with unmatched
capacity and flexibility.
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116
I GENERAL LAB EQUIPMENT
ORDERING INFORMATION
Part Number
Description
RMC24
Refrigerated 24-place micro centrifuge, 230VAC
RMC-24-ROTOR
Rotor, 24x1.5/2ml tubes (included with RCM-24)
RMC-24-1205
Adaptors for individual 0.5/0.6ml tubes, pk of 6
RMC-24-1206
Adaptors for individual 0.25/0.4ml tubes, pk of 6
RMC24$
Refrigerated 24-place micro centrifuge, 110VAC
RMC-24-SS
Strip-spin adaptor for 2x 8x0.2ml PCR strips or
16x0.2ml tubes
RMC-24-1222
Adaptors for individual 0.2ml tubes, pk of 6
GENERAL LAB EQUIPMENT I 117
Rotors & Adaptors:
for 24x1.5/2ml tubes; 2x PCR strip tubes; and up to
individual 0.2 or 0.5ml tubes
• Solid aluminium rotor with
RMC-24-ROTOR
TECHNICAL SPECIFICATIONS
Maximum Speed
13,500rpm
Maximum rcf
17,135 x g
Temperature Range
-10 to +40°C
Timer
0.5-99’; continuous
Dimensions
10.9x17x9.8” / 27.7x43x24.8cm
(WxDxH)
Weight
44lb / 20 kg
Electrical
120V~ or 230V~ 50/60Hz; 500W
features:
sample capacity for 24 ×
1.5/2.0 ml tubes, 2 × PCR strips
or 16x0.2ml tubes
• Maximum speed of 17,135 × g
(13,500 rpm) achieved in <16
seconds
• Rapid-COOL function allows
rotor temperature to reach
4°C within eight minutes; 4°C
maintained even at maximum
speed
• User-friendly LCD display
facilitates temperature control
from -10 to +40°C, and allows
speed to be set either in rpm
or rcf
• Imbalance detection with
automatic shut-off
• Quiet operation at <56dBA at
maximum speed
• Quick spin function ideal for cell
‘pelleting’
• Brushless motor ensures
maintenance-free operation
• Compact with small footprint
• 2-year warranty
RMC-24-SS
Easy access
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
The Cleaver Scientific RMC24
refrigerated micro centrifuge sets a
new standard in laboratory bench top
centrifugation. Its 24-place rotor and
high speed capability make the RMC24
the ideal centrifugation solution for
all molecular biology applications that
entail 1.5ml tubes and 0.2ml tubes
and strips. A self-explanatory LCD
control panel provides full user-control
over speed, time and temperature,
as well as a wipe-clean surface for
easy decontamination, while speed
adjustments in increments as low as
100 rpm or 100 x g ensure precise
control to a maximum 13,500 rpm, or
17,135 x g rcf.
The new RMC24
refrigerated micro
centrifuge
Centrifugation may be either timed
between 30 seconds and 99 minutes
or performed continuously, whereas
a ‘quick’ key is available for short
spins or cell pelleting. The RMC24’s
powerful refrigeration system
maintains sample temperatures as
low as -10°C and takes less than
8 minutes to reach 4°C from room
temperature. A brushless motor drive
allows the rotor to achieve its set
speed quickly and effortlessly, while a
computer-controlled isolation system
ensures that the centrifuge operates
free of vibration with a slight load
imbalance; although shut down occurs
automatically, within 18 seconds
Typical Applications
Centrifugation of samples in a controlled temperature down to -10 degrees C.
and without sample disruption, if a
significant imbalance is detected.
The unique design of the 24-place
rotor allows easy access to all sample
tubes; and individual tube slots within
the solid aluminium rotor support each
tube along its length and retain sample
in the event of tube failure. The rotor
accepts 1.5 and 2.0ml tubes directly
and smaller 0.5ml tubes through
the purchase of specialist adaptors;
whereas an optional StripSpin™
adaptor is connected to the top of
the rotor to spin 0.2ml tubes and
PCR strips. The RMC24 rotor sits
on a tapered shaft so that it may be
removed easily to clean and autoclave.
118
I GENERAL LAB EQUIPMENT
GENERAL LAB EQUIPMENT I 119
features:
features:
• Powerful, reliable motor with optimised
counter balance
• The perfect speed and tilt for blotting and gel
staining
• Circular orbit for effective vortexing at any
speed
• Three-dimensional motion
• CombiCup head accepts a variety of tube sizes
• Designed for use with Blot Boxes
• Versatile head attachment accessories for
microplates and different tube sizes
Blot Boxes
These gel staining/blotting boxes are available in 4 sizes
and are the ideal accessory for incubating blots or staining
gels. The two smaller sized boxes are available in packs of
ten. The smallest box has dimensions of 9.1 x 6.6cm and a
capacity for 3-5ml of solution.
Vortex
Cleaver Scientific’s new variable speed Vortex Mixer combines fast, efficient mixing with minimal vibration. Unlike
other vortex mixers using elliptical orbits, the CSLVORTEX
true circular orbit facilitates uniform sample-vortexing even
at low speed. The CombiHead™ head, which is supplied
as standard, accepts many different tube sizes, while the
unique overmoulding technology used in its manufacture
provides a sturdy plastic core with non-slip TPE that fits
optional heads for microplates, microtubes, PCR strip tubes,
15ml and 50ml tubes and blood vials.
CSLVORTEX may be used in ‘touch’ or continuous mode:
‘touch’ mode being activated by simply depressing the
sample head and then stopped by releasing the pressure.
An optimised counter balance system within the CSLVORTEX minimises vibration and movement of the unit during
operation, whereas its lightweight construction and small
footprint allows it to be readily transported and used in areas
where space is restricted. The CSLVORTEX is also safe for
use in temperature controlled environments, and may be
used within a cold room.
Typical Applications
Mixing of samples in tubes between 0.2ml to 50ml volume.
ORDERING INFORMATION
CSLVORTEX
Cleaver Vortex Mixer with general purpose head
S0200-21
Optional Head Attachment for 24 x 1.5/2.0ml
Tubes,
24 x 0.5ml Tubes and 32 X 0.2ml Tubes (or 4 Tube
S0200-22
Optional Head Attachment for 1 Microplate or
64 x 0.2ml Tubes or 8 x 0.2ml Tube Strips
This is ideal for incubating cut blot strips. The next box in
the range holds 6-10ml of solution and is appropriate for
mini gels and blots up to 11.7 x 8.9cm. Two larger boxes,
available in single packs, can be used for staining gels or incubating blots up to 12 x 12cm or 20 x 16.5cm respectively.
ORDERING INFORMATION
S0200-23
Optional Head Attachment for 8 x 15ml and 8 x
12/13mm Diameter Tubes
CSL3DSHAKER
S0200-24
Optional Head Attachment for 6 x 50ml Tubes
MiniMix 3D Shaker with 20x16.5cm tray and nonslip rubber mat, 230V
Optional Head Attachment for 12 x 1.5/2.0ml
Tubes, Held Horizontally
CSL-BB9X6
Gel/Blot box, 9.1 x 6.6cm, 3-5ml capacity, 10/pack
SO200-25
CSL-BB11X8
S0200-26
Optional Head Attachment for 4 x 15ml Tubes,
Held Horizontally
Gel/Blot box, 11.7 x 8.9cm, 6-10ml capacity, 10/
pack
CSL-BB12X12
Gel/Blot box, 12 x 12cm, 1/pack
CSL-BB20X16.5
Gel/Blot box, 20 x 16.5cm, 1/pack
S0200-27
Optional Head Attachment for 2 x 50ml Tubes,
Held Horizontally
TECHNICAL SPECIFICATIONS
Speed Range
18rpm (115V) / 20 rpm (230V)
Motion
3-D, nutating
Tilt Angle
5°
Operating Modes
Touch or Continuous
Platform Size (w x d)
20x16.5cm
Ambient Operating Range
4 - 65°C
Ambient Operating Range
4-65°
Dimensions (w x d x h)
14cm x 16cm x 13cm
Dimensions (w x d x h)
20.3X17.8X10.5cm
Weight
2.2Kg
Weight
0.88kg
Electrical
115V or 230V, 50/60Hz
Electrical
115/230V 50/60Hz
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The Cleaver Scientific MiniMix combines the motions of orbital shaking and rocking to produce a gentle, but thorough,
3-D action that is perfect for antibody incubation of western
blots and staining gels.
The pitch and speed of the Cleaver Scientific MiniMix are
optimised to generate a tilt angle that is large enough to
ensure uniform distribution of solutions, yet small enough
to prevent the centre of the blot from drying out. This has
the added benefit of allowing users to work with minimal
volumes, thus conserving valuable probes and antibodies.
A non-slip rubber mat keeps the boxes in place on the agitating platform, which, along with the housing, is very easy
to clean and decontaminate after use. The MiniMix’s compact and light weight design allows it to be moved around
the lab where needed. It is also safe for use in an incubator
or cold room for temperature controlled reactions.
TECHNICAL SPECIFICATIONS
115V 0 - 3400 RPM
230V 0 - 2850 RPM
Speed Range
MiniMix
3D Shaker
Typical Applications
Staining and destaining of gels. Incubating blots.
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120
I GENERAL LAB EQUIPMENT
GENERAL LAB EQUIPMENT I 121
features:
features:
• Compact, fits in the palm of your hand
• Microprocessor control with digital
performance for precise, accurate control
• Exchangeable blocks, for tubes 0.2 to 50ml
• Clear cover ensures temp, uniformity
• Digital temperature control
• Portable (Optional 12V car adapter)
• LCD screen showing timer and temperature
simultaneously
• Outsranding heating rate
• User temperature calibration
• Single molded chamber, no cracks or welds
• Data logging and function control software
available
microBLOCK
A breakthrough in size and economy, our NEW microBLOCK Mini Dry Bath is truly the first personal block incubator. Whether your customers are incubating PCR strips or
tube sizes up to 50mL, the compact microBLOCK Mini has
the smallest footprint of any digital dry bath on the market.
MDMINI-B01
MDMINI-B04
MDMINI-B02
ORDERING INFORMATION
microBLOCK Digital Dry Bath, with block lifter (No
block supplied)
MD-MINI-B01
For 0.2ml tube (PCR Strip Tube), 32 wells, 6.35mm,
depth 19mm, L71 x W47 x H32mm
MD-MINI-B02
For 1.5ml tube, 12 wells, 10.8mm, depth 28.5mm,
L71 x W47 x H32mm
SINGLE BLOCK
MD-MINI-B03
For 15ml tube, 6 wells, 17.3mm, depth 70mm, L71
x W47 x H75mm
TCDB-01
MD-MINI-B04
For 50ml tube, 2 wells, 29.2mm, depth 72mm, L71
x W47 x H75mm
MD-MINI-B05
For 0.5ml tube, 12 wells, 8.0mm, depth 25mm, L71
x W47 x H32mm
MD-MINI-B06
For 2.0ml or 1.5ml tube, 12 wells, 11.0mm, depth
30mm, L71 x W47 x H32mm
MD-MP01-S
MD-MINI-B07
For 1.5ml tube, 12 wells, 10.9mm, depth 30mm,
L71 x W47 x H32mm
MD-MP02-S
MS-BL95-E
Block lifter 95mm, with E-Type Retaining Rings
MD-MP01-D
Typical Applications
Precise heating of samples up to 80°C in an ultra compact
bath.
ORDERING INFORMATION
The Cube Dry Bath Incubator (one block unit);
without block 220V
DUAL BLOCK
TCDB-02
The Cube Dry Bath Incubator (dual block unit);
without block 220V
Accessories
Temperature Range
Ambient +5 to 80 °C
Temperature Accuracy
+/- 0.5 °C
Temperature Increments
0.1 °C
Temperature Uniformity
+/- 0.2 °C
Dimensions
11 x 15 x 10 cm
MD-B0.2
Weight
500g
MD-B0.5
Block for Microplate; Titerplate Plain Block for
Single Block Unit Only
Block for 96 wells deep Microplate or PCR plate
for Single Block Unit Only
Block for Microplate; Titerplate Plain Block for
Dual Block Unit Only
Block for 96 wells deep Microplate or PCR plate
for Dual Block Unit Only
Double side block: one side for 1.5 or 2.0 ml
tube, 20 wells;
Opposite side for 0.5 ml tube, 30 wells
Combination block : for 0.5 ml tube, 12 wells and
for 1.5 or 2.0 ml tube, 12 wells (on the same side)
Block for 0.2 ml tube, 64 wells or for 0.2ml PCR
strip tubes for 8 wells x 8
Block for 0.5 ml tube, 20 wells
Electrical
100-240V, 50-60Hz*
MD-B1.5
Block for 1.5 ml tube, 20 wells
MD-B13
Block well size 13 mm, 20 wells
MD-B17
Block for 15 ml centrifuge tube, 12 wells
MD-B20
Block well size 20 mm, 12 wells
MD-B25
Block well size 25 mm, 6 wells
MD-B29
Block for 50 ml centrifuge tube, 4 wells
MD-RS232
RS232 Cable
MD-DLSW
Single License Software Package for Datalogging
Single License Software Package for Datalogging
and Computer-controlled Temperature Setting
MDMINI-B05
MD-MP02-D
MD-B0.5/1.5
MD-B0.5+1.5
MDMINI-B07
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FOR MORE INFORMATION
Cube Dry Baths
Cleaver Scientific’s Cube digital dry baths are available in
single and dual block models, and comprise a comprehensive range of interchangeable blocks. Each digital dry bath is
compact, easy-to-use and excellent value for money.
MBDB-01
TECHNICAL SPECIFICATIONS
MDMINI-B03
• Rapid temperature increase rate
• User tempreature calibration
• PTFE coated chamber resistance stains
A personal sized drybath
• Wide temperature control range with
excellent uniformity
MD-PCSW
Typical Applications
Precise heating of samples up to 150°C in a compact bath .
Interchangeable quick-change blocks have fast heat-up
times and reproducible temperature uniformity and accuracy, and may be used in a variety of applications, which
include: restriction digestion, BUN, melting agar, coagulation
studies, hybridisation, Hot Start PCR® reactions and DNA
denaturation. Because of an impermeable moulded aluminium alloy block construction, each Cube dry bath may also
be adapted as a mini water bath incubator if desired.
Both dry baths incorporate a digital microprocessor controller
for accurate temperature control in 0.1°C increments from
ambient +5°C to 150°C. Rapid and easy programming is
facilitated by the easy to use arrow keys on the sloped front
panel, while both the temperature and running time are
shown simultaneously on the conspicuous dual digital LCD
display. Two software options are available for data logging
to a PC and computer control (please enquire).
MD-MP01-S
MD-MP02-S
MD-MP01-D
MD-MP02-D
MD-B0.5/1.5
MD-B0.5+1.5
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122
I GENERAL LAB EQUIPMENT
GENERAL LAB EQUIPMENT I 123
ORDERING INFORMATION
TECHNICAL SPECIFICATIONS
GTC96S
GTC96S thermal cycler with 96-well block, 240VAC
Sample Capacity
1x 96-well plate; 12x 8x0.2ml strip tubes;
96 x 0.2ml tubes
GTC96$
GTC96S thermal cycler with 96-well block, 120VAC
Programmable
Temperature Range
4-99.9°C
features:
Package Deals
Temperature Control
Calculated or block
CSL-PCRKIT
PCR package includes GTC96S thermal cycler,
MSMIDI96 96-well electrophoresis unit and
nanoPAC-500 power supply
Temperature Accuracy /
Uniformity
±0.5°C /
±0.5°C
• Compatible with 96-
Heating / Cooling Method
Peltier
Complete PCR package with low cost clean room.
Includes CSL-GTC96S, CSL-UVCAB, CV2, CV20,
CV200, CV1000 and CV8-200 pipettes, MSMIDI96
and nanoPAC-500.
Maximum Heating /
Cooling Rate
5°C / 3.5°C per second
Temperature Range of
6-Segment Blocks
30-99°C; temperature of each segment
may be set independently
Maximum Temperature
Difference Between
6-Segment Blocks
24°C
6-Segment Temperature
Block Format
6 segments, each 4x4-well
Programmable Lid
Temperature
60-65°C, 90-94°C
Memory
200 complete programmes
Temperature Increments /
Decrements
Yes
Time Increments /
Decrements
Yes
CSL-CLEANCAB
Use CSL-PCRKIT$ and CSL-CLEANCAB$ to order 120VAC versions
well plates, 0.2ml tubes
and tube strips
• Protocol optimization
selectable from 1 to
24°C across the entire
temperature control
range from 4-99°C
• Improved protocol
optimisation aided by
Flexible Temperature
technology allows
simultaneous testing of
6 different annealing
temperatures for
optimum yield of the
desired PCR product
less nonspecific
artefacts
• Precision temperature
control increases both
speed and efficiency
• Intuitive programming
through selfexplanatory userfriendly interface
• 3-year warranty
The NEW GTC96S
Thermal Cycler at a Personalised price!
Intuitive user-friendly interface facilitates
manual selection of different temperatures
in each of the 4x4-well zones, for full user
control during PCR optimisation.
Flexible temperature technology provides
ability to run multiple samples with
different annealing temperatures in one
run. This is ideal for genotyping.
Blue and black squares on the thermal
cycler block aid identification of each of the
six 4x4-well temperature zones.
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Faster and with enhanced features,
the new GTC96S advanced
thermal cycler delivers exceptional
performance at an affordable price.
An improved protocol optimisation
process utilises Flexible Temperature
technology to segregate the 96-well
plate into six discrete (4x4-well)
temperature zones, made easily
distinguishable by blue and black
squares. Temperature selection is no
longer automated and is entirely in
the hands of the operator over a 24°C
advanced range, anywhere between
4 and 96°C. This enables the operator
to optimise PCR by testing 6 different
temperatures simultaneously in just
one thermal cycler run. This is ideal for
Genotyping work.
With heating and cooling rates of
5°C/s and 3.5°C/s respectively, the
precision temperature control of
the GTC96S minimises temperature
overshooting and undershooting
between individual stages within each
PCR cycle, resulting in faster run times
and greater efficiency.
Programming is both quick and simple
through a large user-friendly interface,
while pre-programmed methods for
Optimisation, Touchdown and Time
Increments make set-up obvious
Typical Applications
Gene cloning and analysis. Gene expression analysis. Mutational screening
even to first time users. A heated
lid, which is fully adjustable to apply
optimal pressure to 0.2ml tubes and
microplates, may be programmed to
hold different temperatures between
60 and 65°C and 100 to 115°C.
Additional advantages offered by the
new heated lid are its slide-in slideout design for safe access to samples,
and its prevention of condensation
formation during overnight cooling.
124
I GENERAL LAB EQUIPMENT
GENERAL LAB EQUIPMENT I 125
Peristaltic
Pumps
features:
Heaters and
Stirrers
Cleaver Scientific hotplates, stirrers and hotplate-stirrers
feature an exceptionally durable, chemically resistant, white
ceramic work surface. Their space-efficient design (20 x 23
cm footprint), makes them ideal for use on crowded bench
tops and inside cabinets.
Advanced microprocessor controls with convenient turn
knobs allow quick, precise adjustment and maintenance of
speed and temperature. A safety indicator LED on each front
panel indicates when the heating and stirring functions have
been activated. With a square 19 x 19 cm work surface, all
three models are compatible with a wide variety of popular
sizes of borosilicate glass beakers, flasks, bottles and other
vessels.
The hotplate/magnetic stirrer model (CSL Hotplate) comes
complete with a support rod for mounting thermometers
and temperature probes.
Typical Applications
Heating and stirring of liquids in beakers and conical flask.
• Large work surface, 19 x 19 cm
• Space saving design, 20 x 23 cm Footprint
• Chemical resistant white ceramic top plate
• Safety LEDs indicate when heating or stirring
• Quick and easy adjustment of heating/mixing
ORDERING INFORMATION
CSL-HOTPLATE
CSL Hotplate, 19 x 19 cm
CSL-HOTSTIR
CSL Hotplate Magnetic Stirrer 19 x 19 cm
CSL-STIR
CSL Magnetic Stirrer, 19 x 19 cm
Stirring Water
Baths
A powerful magnetic stirring mechanism combined with high
wattage heating allows each Cleaver Scientific stirring water
bath to maintain temperatures to a maximum 99°C. Available in 10 and 20 litre bath capacities, these water baths
comprise as many as 3 stirrers for a maximum stirring speed
of 1800rpm. Each bath includes a conspicuous front-panel
LCD, reproducible microprocessor control of temperature
within 0.1°C increments, a corrosion resistant stainless steel
interior and automatic alarm and safety shutdown mechanism. Optional data logging and PC control software is also
available.
Use $ sign to order 110v version
TECHNICAL SPECIFICATIONS
Speed Range:
60-1500 rpm (stirring units only)
Temp. Range
(heating units only)
Ambient +5° to 380°C
Platform
19 x 19cm
Control
Quick Adjustment Knobs
ORDERING INFORMATION
Available in single, dual and tetrad formats these versatile peristaltic pumps are an ideal accessory for gradient gel formation
with the VS20-DGGE. The ‘easy load’ head of the MU-D01
single peristaltic pump accommodates multiple tubes of
different diameters offering the user enhanced flexibility in
flow rate selection, while the MU-D02 single pump has the
added benefit of a more powerful pump motor. The MFU-01
and MFU-02 dual and tetrad pumps are equipped with microprocessor technology for more accurate rpm control, and
comprise 2 and 4 peristaltic pumps respectively that may be
controlled individually. All 4 pumps are reversible and have a
small footprint, occupying minimum space within the laboratory. Other applications include: filtration, sampling, chemical
spraying, dispensing, transferring, feeding and filling.
Ordering Information
MU-D01 Single Peristaltic Pump
MU-S14
Silicon tube I.D. 1/16”,
25 ft
MU-D02 Single Peristaltic Pump
MU-S16
Silicon tube I.D. 1/8”,
25 ft
MFU-01
Dual Peristaltic Pump
MU-S18
Silicon tube I.D. 3/8”,
25 ft
MFU-02
Tetrad Peristaltic Pump MU-S25
MU-S13
Silicon tube I.D. 1/32”,
Silicon tube I.D. 1/4”,
MU-S17
25 ft
25 ft
Silicon tube I.D. 3/16”,
25 ft
TECHNICAL SPECIFICATIONS
SWB-10L-1
Stirring Water Bath 10L with 1 built-in stirrer
SWB-10L-2
Stirring Water Bath 10L with 2 built-in stirrers
MU-D02
MFU-01
MFU-02
SWB-20L-1
Stirring Water Bath 20L with 1 built-in stirrer
No. of Heads 1
1
2
4
Stirring Water Bath 20L with 3 built-in stirrers
Max. RPM:
300
600
100
100
Flow Rate, :
ml/min
1.2-1140
0.3-2280
0.6-380
0.6-380
SWB-20L-3
All of the above include lid
Accessories
MU-D01
Unit Dimensions (w x d x h) 20 x 23 x 11.5 cm
SWB-LID10
Transparent lid for 10L stirring water bath
Dimensions:
(h x l x w)
20x34x13
20x34x13
26.5x34x18
26.5x34x18
Electrical Data
SWB-LID20
Transparent lid for 20L stirring water bath
Weight, kg:
5.7
5.7
5.7
5.7
120V, 60 HZ / 230V, 50/60 HZ
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Typical Applications
Precise temperature control and stirring of liquids in beakers
and flasks. Precise pumping or dispensing of small liquid
volumes.
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126
I GENERAL LAB EQUIPMENT
TECHNICAL SPECIFICATIONS
GENERAL LAB EQUIPMENT I 127
TECHNICAL SPECIFICATIONS
for all models – CSL-NHYBRID -BASIC, VX, ORB, REC & ROC
Display
3.5” 64K colour-TFT display
Incubator Model
Controller
32-bit Microprocessor-control
for Shaking-platform models only
Shaker motion
Speed
CSL-NHYBRIDVX
Vortex
50-1500rpm
Touch screen & Graphical interface
CSL-NHYBRIDORB
0-200rpm
Continuous; Programmable 1-9999’ with
alarm / 1’
Orbital (clockwise
&anticlockwise)
CSL-NHYBRIDREC
Reciprocal
5-100rpm
Temperature Control
Range / Resolution
Ambient +5°C to 85°C / 0.1°C
CSL-NHYBRIDROC
Rocking
5-100rpm
Temperature Uniformity /
Accuracy at 37°C
±0.2°C
Temperature Calibration
Yes
Control interface
Timer / Resolution
Dimensions (w x d x h)
Inner Chamber
Exterior
ORDERING INFORMATION
CSL-NHYBRIDORB & CSL-NHYBRIDREC incubators
34x22.5x26cm
44.2x46.2x45.2cm
Platform Dimensions
27x20cm
(20x30cm for CSL-NHYBRIDVX)
Data-logging capacity
RS-232
Operating Voltage
110/220V~ 50/60Hz (Dual, selectable)
Construction / Weight
Painted metal chassis; ABS front door
panel / 29kg
Safety features
Safety-door & thermal safety switches;
auto shut-off upon fan failure;
auto-recovery after power failure
ORDERING INFORMATION
CSL-NHYBRIDBASIC incubator
CSLNHYBRIDBASIC
Incubator with 2x stainless steel mesh-shelves –
110/240 VAC
Accessories
CSL-HYB-SSMP
1x Stainless steel mesh plate, 32.5 x 34.5cm, with
4x holders
CSL-NHYBRIDVX & CSL-NHYBRIDROC incubators
CSLNHYBRIDVX
Vortex Incubator with 20x30cm platform for 4
microplates (no rotisserie) – 110/240 VAC
CSLNHYBRIDROC
Rocking-shaker Incubator with 27x20cm platform
(no rotisserie) – 110/240 VAC
Hybridisation Rotisserie Options
CSL-HYB-8RT
1x Rotisserie for 8x40mm glass tubes (CSLHYBBT40X150, CSL-HYBBT40X200 & CSLHYBBT40X300; tubes not included)
CSL-HYB-16RT
1x Rotisserie for 16x50ml disposable conical tubes
(tubes not included)
CSL-HYB-24RT
1x Rotisserie for 24x15ml disposable conical tubes
(tubes not included)
CSLNHYBRIDORB
Orbital-shaking Incubator with 27x20cm platform
(no rotisserie) – 120/240 VAC
CSLNHYBRIDREC
Reciprocal-shaking Incubator with 27x20cm
platform (no rotisserie) – 110/240 VAC
Hybridisation Rotisserie Options
CSL-HYB-8RT
1x Rotisserie for 8x40mm glass tubes (CSLHYBBT40X150, CSL-HYBBT40X200 &
CSL-HYBBT40X300; tubes not included)
CSL-HYB-16RT
1x Rotisserie for 16x50ml disposable conical tubes
(tubes not included)
CSL-HYB-24RT
1x Rotisserie for 24x15ml disposable conical tubes
(tubes not included)
Accessories
CSLNHYB-P2720
Additional 27x20cm platform (with 4x 10cm pillars)
to double capacity of CSL-NHYBRIDORB & CSLNHYBRIDREC incubators
CSLNHYBFH-250
1x250ml flask holder for CSL-NHYBRIDORB &
CSL-NHYBRIDREC platforms
CSLNHYBFH-500
1x500ml flask holder for CSL-NHYBRIDORB &
CSL-NHYBRIDREC platforms
CSL-NHYBFH250-SET
5x250ml flask holders for CSL-NHYBRIDORB &
CSL-NHYBRIDREC platforms
CSL-NHYBFH500-SET
4x500ml flask holders for CSL-NHYBRIDORB &
CSL-NHYBRIDREC platforms
CSL1x Glass tube 40x150mm (d x l) for CSL-HYB-8RT
HYBBT40X150
CSL1x Glass tube 40x200mm (d x l) for CSL-HYB-8RT
HYBBT40X200
CSL1x Glass tube 40x300mm (d x l) for CSL-HYB-8RT
HYBBT40X300
e.g. To purchase an orbital incubator with one 8-tube rotisserie, an
extra 27x20cm platform and four 500ml flask holders, use codes
(quantities): CSL-NHYBRIDORB (1 off), CSL-HYB-8RT (1 off), CSLNHYB-P2720 (1 off) and CSL-NYHYBFH-500-SET (1 off)
e.g. To purchase a vortex incubator with one 8-tube rotisserie and one
16-tube rotisserie, use codes (quantities): CSL-NHYBRIDVX (1 off),
CSL-HYB-8RT (1 off) and CSL-HYB-16RT (1 off)
Accessories
CSLHYBBT40X150
1x Glass tube 40x150mm (d x l) for CSL-HYB-8RT
CSLHYBBT40X200
1x Glass tube 40x200mm (d x l) for CSL-HYB-8RT
CSLHYBBT40X300
1x Glass tube 40x300mm (d x l) for CSL-HYB-8RT
CSL-HYT-8RT
CSL-NHYB-P2720
CSL-HYT-16RT
Various Glass Tubes
CSL-HYT-24RT
CSL-NHYBFH-250-SET
features:
• Five compact benchtop
incubator models for multiple
temperature-sensitive
applications, ranging from
incubation of overnight
cultures to nucleic acid
hybridisation
• Large 3.6” colour touchscreen
control panel with easy-toprogramme 3-line display
• Temperature uniformity to
within ±0.2°C
• Wipe-clean painted metal
chassis and stainless steel
interior
With a compact, space-saving stackable design and temperature uniformity
to within ±0.2°C, Cleaver Scientific’s
hybridPRO can be used for numerous
temperature-dependent applications.
In addition to the standard entry level
model, the hybridPRO range includes
four incubator models, each supplied in
one of four shaking-platform formats
– vortex, orbital, reciprocal or rocking
– that may be customised for nucleic
acid hybridisation techniques with three
rotisserie options. A large 3.6” colour-touchscreen control panel simplifies
manipulation of speed, temperature
and time within an easy to programme
3-line display, while a new 32-bit
microprocessor provides the temperature uniformity and stability necessary
to support the most temperature-sensitive applications. A modern stylish
front door panel encloses the stainless
steel interior of a robust metal chassis
designed to withstand the stresses of
frequent daily usage.
hybridPRO Hybridisation
Shaking Incubators
The hybridPRO Incubator
Range includes…
CSL-NHYBRIDBASIC – Standard
• Basic Incubator model with 2 stainless steel mesh-shelves to maintain
airflow
• Typical applications: drying agar
plates & microbial plating techniques
CSL-NHYBRIDVX - Vortex
• Vortex Incubator model with vibrating platform for 4x microplates
exhibits orbital, reciprocal or rocking
motion
• Optional flask holders fit to the
shaking platform to accommodate up
to five 250ml or four 500ml flat-bottomed flasks
• Shaking platform may be replaced by
one of three optional hybridisation
rotisseries
• Additional platform with four 10cm
mounts available to double capacity
in orbital and reciprocal models
• Typical applications:
• Three rotisserie options for hybridisation techniques using 40mm, 15ml
and 50ml tubes
– CSL-NHYBRIDORB - growing
overnight cultures of bacteria,
yeast & insect cells;
• Typical applications: drying agar
plates & microbial plating techniques
– CSL-NHYBRIDREC - staining,
blocking & antibody incubations;
CSL-NHYBRIDORB – Orbital,
CSL-NHYBRIDREC – Reciprocal,
CSL-NHYBRIDROC - Rocking
– CSL-NHYBRIDROC - nucleic acid
hybridisations: northern & Southern blotting techniques.
• Each model equipped with a
27x20cm shaking platform that
Typical Applications
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Temperature controlled incubation with a choice of orbital, reciprocal, rocking or vortex sample mixing and shaking.
128
I GENERAL LAB EQUIPMENT
GENERAL LAB EQUIPMENT I 129
features:
• Inactivates nucleic acids in 5 to 30 minutes
• Doors fitted with safety switches
• Complete with four powerful, timer
controlled UV bulbs
• Efficient decontamination of the complete
work surface
• Suitable for work with 32P
UV Sterilisation
Cabinets
These UV Sterilisation Cabinets provide a convenient
area for setting up PCR reactions in a nucleic acid free
environment thus limiting PCR reaction contamination.
Acting effectively as a low cost alternative to a clean room,
the powerful UV lights on the cabinets denature nucleic
acids in 5 to 30 minutes making them unsuitable for
amplification. The cabinet incorporates safety features to
prevent user exposure to UV light. The UV lights themselves
are timer controlled and there are safety switches on the
cabinets doors which turn off the UV lights when opened.
The cabinets also act as an efficient shield of beta radioactive
emissions and can therefore be safely used with isotopes
such as 32P.
ORDERING INFORMATION
CSL-UVCAB
UV sterilisation cabinet with timer, four UV lights
and white light, no Tray
CSL-UVCABTY4
UV Sterilisation cabinet with timer, four UV lights
and white light and includes tray.
CSL-UVCABMINI
Mini UV sterilisation cabinet with timer, UV light
and white light, no Tray
Mini UV sterilisation cabinet with timer, UV light
CSL-UVCABMTY4
and white light, includes Tray
CSL-UVTUBE
Replacement UV tube for cabinets
CSL-WHITETUBE
Replacement White Light tube for cabinets
CSR-TW4
General Purpose Tray White - 68 x 54cm
CSR-TY4
Radiation Hazard Tray Yellow - 68 x 54cm
With the benefits comparable to those offered by the CSLUVCAB, the new Mini UV Cabinet is the perfect solution for
laboratories constrained by space and budget. A reduced
height and footprint allows the Mini UV Cabinet to fit snugly
and conveniently into those small areas within the laboratory
that would otherwise remain unused.
CSR-TL4
Liners - 68 x 54cm, Pk/25 (for cabinet)
Ideally suited to the measurement of electrophoresis buffer
pH, Consort pH meters may also be used to measure
conductivity, total dissolved salt, salinity and temperature.
Each meter may be supplied on its own, with or without the
electrode, calibration buffers and standards. All meters comprise a BNC connection for connection to most electrodes
commonly available on the market.
ORDERING INFORMATION
technical specifications
C1010
Meter only (without electrodes) + mains adaptor
Temperature
0°C for up to 4 hours
C1010P
Meter kit for pH: meter + pH/ATC electrode SP10T
+ 2x50 ml buffers (pH 4 and 7) + 50 ml electrolyte
(3M KCl)
Capacity
Meter kit for conductivity: meter + conductivity/
ATC electrode SK10T + 50 ml conductivity standard
(0.01 M KCl)
36 x 1.5ml
96 x 0.2ml
12 x 0.2ml PCR Strips
1 x 96 well PCR Plate
Dimensions:
142 x 142 x 63.5mm
Weight:
900g
Material:
Durastar Polymer
C1010T
Meter kit complete: meter + pH/ATC electrode
SP10T + conductivity/ATC electrode SK10T +
dissolved oxygen electrode SZ10T + 2x50 ml buffers
(pH 4 and 7) + 50 ml electrolyte (3M KCl) + 50 ml
conductivity standard (0.01 M KCl)
SH300
Flexible electrode holder (optional)
C5010
Meter only (without electrodes) + 4 NiMH batteries
+ mains adaptor
C5010X
Meter kit without electrodes: C5010 + 2x50 ml
buffers (pH 4 and 7) + 50 ml electrolyte (3M KCl) +
50 ml conductivity standard (0.01 M KCl) + carrying
case
C5010P
Meter kit for pH: C5010 + pH/ATC electrode SP10T
+ 2x50 ml buffers (pH 4 and 7) + 50 ml electrolyte
(3M KCl) + carrying case
C5010K
Meter kit for conductivity: C5010 + conductivity/
ATC electrode SK10T + 50 ml conductivity standard
(0.01 M KCl) + carrying case
C5010Z
Meter kit for oxygen: C5010 + dissolved oxygen
electrode SZ10T + carrying case
C5010T
Meter kit complete: C5010 + pH/ATC electrode
SP10T + conductivity/ATC electrode SK10T +
dissolved oxygen electrode SZ10T + 2x50 ml buffers
(pH 4 and 7) + 50 ml electrolyte (3M KCl) + 50 ml
conductivity standard (0.01 M KCl) + carrying case
For 110 V AC, please add ‘-$’ as a suffix to the appropriate code
TECHNICAL SPECIFICATIONS
UV Lights
Maxi
4 x 15 Watt
Mini
4 x 15 Watt
White Light
Maxi
15 Watt
Mini
15 Watt
Dimensions
(h x w x d)
Maxi
Mini
770 x 560 x 420mm
510 x 560 x 350mm
Weight
Maxi
19 Kg
Mini
12 Kg
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OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Coolcube
The CoolCube is designed to store samples safely on the lab
bench without sample degradation caused by temperature
increase or fluctuation. The CoolCube maintains temperature at approximately 0°C for up to four hours. Simply place
the CoolCube in a freezer overnight and it will be ready to
use. One side of the CoolCube can be used as a convenient
PCR workstation for 12 standard 1.5/2.0ml microtubes with
one 96 well PCR plate, or with 12x 0.2ml strips or 96x0.2ml
tubes. The other side may be used to hold up to 36 standard
1.5/2.0ml microtubes.
C1010K
White light provides excellent visibility when working within
the cabinets. For use with trays of size 68 x 54cm, CSRTW4/TY4/TL4.
Optional HEPA Filter extraction system available suitable for
removal of VOC’s/Solvent fumes and particulate – please enquire.
Consort
pH Meter
A4049
Car adaptor, 12 V (optional)
Typical Applications
Measuring pH, conductivity, salinity and temperature of
buffers. Cool storage of small and large samples
Ice
Buckets
Cleaver Scientific Ice Buckets are constructed of durable plastic
and offer both robustness and long life. They will also withstand reasonable use with dry ice. Lids are available for the the
large bucket.
ORDERING INFORMATION
CSLIB
Large Ice Bucket - White
CSLIBLID
Lid For Large Ice Bucket - White
CSMIB
Mini Ice Bucket - White
CSL-COOLCUBE Coolcube Storage Rack
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OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
130
I GENERAL LAB EQUIPMENT
GENERAL LAB EQUIPMENT I 131
CSL-Chiller
The CSL-CHILLER is available ready assembled with the
thermostat mounted on the refrigerator and supplied with
insulated tubing and clips to form a system ready to use.
Supported by an industry leading 3 year warranty emphasising our confidence in the reliability of our product.
The system comprises the following:
• 5 litre tank with drain tap
• relay control for refrigeration on/off
ORDERING INFORMATION
• cooling power 140W @ 0°C
Gel Dryer
• temperature range -20 to 100°C
CSL-GDVH
Midi Gel Dryer 220V, 21 x 31 cm
CSL-S2131
Replacement Silicon Rubber, Midi
CSL-M2131
Replacement Supporting Mask, Midi
CSL-GDVH35
Maxi Gel Dryer 220V, 35 x 45 cm
CSL-S3545
Replacement Silicon Rubber, Maxi
CSL-M3545
Replacement Supporting Mask, Maxi
CSL-GDVHCS
Cellophane sheets, precut, 30x30cm, pk/100
CSL-GDVH35CS
Cellophane sheets, precut, 35x45cm, pk/100
The immersion thermostat incorporates Intelligent Control Optimisation for adaptive intelligent PID temperature
control and includes a powerful integral pump, making the
system suitable both for immersing samples and circulating
temperature controlled fluid to external devices. It includes a
convenient timer function for reaction timing and a variable
high temperature alarm setting. The temperature range and
heater power are automatically limited according to the
liquid type selected.
Pump
CSL-GDPUMP
Geldryer Pump - 732502 MZ 2C NT +2AK
TECHNICAL SPECIFICATIONS
Gel Dryer
Temperature Increment
• Simple-to-use rotor plus two keys provide access to the
interactive interface for fast, accurate set-up
0.1°C
Temperature Calibration Yes
• Clear digital display for instant reassurance - easy to read
from a distance
Temp Uniformity
± 0.2°C
Timer
1-999 mins
Drying area
Midi
Maxi 21 x 31cm
35 x 45cm
• Visual and audible alarm - alert you when your attention is
required
Operating Temp. Range Ambient to 90°C
• Operating setpoint plus 3 adjustable preset temperatures
for convenience
Pump MZ 2C + 2 AK
Dimension (w x h x l)
• User calibration facility for optimum accuracy at the required operating temperature
• Powerful integral pump - maximum flow rate 17L/min
• Cooling/heating range: -20 to 100°C
Typical Applications
Temperature control of electrophoresis and other laboratory
equipment
30 x 36 x 8cm
44 x 50 x 8cm
Unit Dimensions
(w x l x h)
24.1 x 34.5 x 32.6cm
Vacuum Pump
Chemistry Diaphragm Pump
Number of Stages
2
Pumping Speed 50/60
Hz
1.9m3/h / 2.1 m3/h // 1.2cfm
Ultimate Vacuum (total) 9 mbar // 6.8 Torr
• Stability ± 0.1°C
Note: The refrigeration unit can be switched off indepedendently of the thermostat to allow heating only applications. All the refrigeration base units can be used up to a
maximum temperature of 100°C.
Midi
Maxi
ORDERING INFORMATION
CSL-CHILLER
Chiller system, -20 to 100°C, including insulated
tubing & clips, 220V supply
CSL-CHILLER$
Chiller system, -20 to 100°C, including insulated
tubing & clips, 115V supply
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Ultimate Vacuum with
Gas Ballast
15 mbar // 11 Torr
Inlet Connection (IN)
Hose nozzle NW 10mm
Outlet Connection (EX)
Hose nozzle NW 10mm
Weight
11.9kg
Pump Flow Rate
35 l/min
Typical Applications
Drying of sequencing, IEF or smaller format gels for analysis,
storage and documentation
Gel Dryers
With a drying area of 21 x 31 cm, the Midi gel dryer can dry
six 10 x 10cm gels or a single larger gel. The Maxi Gel Dryer
with a 35 x 45cm drying area can dry twelve 10 x 10cm
mini gels simultaneously. The unit’s microprocessor controls
temperature and time, each parameter being displayed on
its own LED display. The gels are heated from the base plate
while the vacuum removes the moisture from below to dry
the gel homogeneously. These dryers feature optimal sealing
using a silicone rubber cover and supporting mask. When
applying the vacuum, a groove that frames the drying surface provides an optimal tight seal during drying.
Vacuum Pumps
The Vacuubrand® MZ 2C + 2 AK is a quiet, low maintenance oil free vacuum pump, which generates the lowest
pressure measurable reproducibly at room temperature for
almost 100% efficient solvent recovery. Two round bottomed flasks collect and condense noxious gases released
during gel drying, whereas inlet and outlet separators act
in tandem with the diaphragm pump to facilitate controlled
collection of vapours without risk of condensate entering
and contaminating the pump. The pump also benefits from
a compact design and construction from specially-selected chemically resistant materials, long-life easy-to-change
diaphragms and valves, and gas ballast function.
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
132
I GENERAL LAB EQUIPMENT
GENERAL LAB EQUIPMENT I 133
ORDERING INFORMATION
CS-NOR
Orbital shaker with 30x30cm platform and nonslip rubber mat – 110 / 230 VAC
CS-NRC
Reciprocal shaker with 30x30cm platform and
non-slip rubber mat – 110 / 230 VAC
CS-NRK
Rocking shaker with 30x30cm platform and nonslip rubber mat – 110 / 230 VAC
TECHNICAL SPECIFICATIONS
Shaker Model
CS-NOR
CS-NRC
CS-NRK
CW-23
Orbital:
singledirection or
alternating,
bi-directional
clockwise &
anticlockwise
shaking
Linear,
reciprocating
action
Rocking
Gyratory
(2-D or
3-D)
Orbits per
Shaking Cycle
0.1-10
-
-
-
Orbit /
Amplitude
20mm
19mm
-
-
-
-
12°
8°
Motion
CS-NOR, CS-NRC & CS-NRK Options
CS-P3030
1x 30x30cm shaking platform with 8 adjustable
pillars and non-slip rubber mat
CS-SP
2x Strip springs to secure laboratory glassware,
tissue culture flasks & multi-well plates
MS-DIMPLED-30
1x 30x30cm dimpled mat
2-D/3-D Gyratory Shaker
CW-23
2-D/3-D gyratory shaker with 33x33cm platform
and non-slip rubber mat – 110 / 230 VAC
CW-WB
Hybridisation water bath (ambient to 95°C) with
RS232 port for data-logging
CW-SP
2x Strip springs to secure laboratory glassware,
tissue culture flasks & multi-well plates
CW-P3333
1x 33x33cm shaking platform with 8 adjustable
pillars and non-slip rubber mat
CW-DIMPLED
1x 33x33cm dimpled mat
MW-PF-SS
Stainless Steel Platform Option per platform for 2
& 3D Waver Shaker
Max. Tilt
Angle
Speed /
Resolution
features:
0-200rpm /
1rpm
1-9999’ with alarm; continuous / 1’
Controller
Digital microprocessor
Display
4-digit red LED
Operating
Temperature
4-40°C
Optional
Stacking
Platform
Yes
Platform
Dimensions
(w x l)
Max. Load
for Platform
30 x 30 cm
10kg
Unit
Dimensions
(w x l x h)
Operating
Voltage
15kg
33 x 33 cm
15kg
26 x 31
x 13 cm
Operating
Power
Weight
• 2-D/3-D shaker supplied
with 33x33cm shakingplatform & non-slip rubber
mat, to accommodate
optional hybridisation water
bath
5-100rpm / 1rpm
Timer /
Resolution
• Orbital, reciprocal & rocking
models supplied with a
30x30cm shaking-platform
& non-slip rubber mat
15kg
33 x 45
x 28 cm
• Additional platforms
available for all models to
double capacity without
increasing footprint area
• Dimpled mat option (all
models) to support 1.5ml,
15ml and 50ml tubes
110 / 220V
7 kg
7 kg
8 kg
Rockers and Shakers
10 kg
110/240V selectable
Cleaver Scientific rockers and shakers
are available as four different models in
orbital, reciprocal, rocking and 2-D/3-D
gyratory shaking-formats. Features and
benefits include: outstanding uniform
motion and low noise; microprocessor-based keypads with digital control
and display of pre-set time, continuous
time, operation mode and speed; and
high quality stain-resistant platforms
with non-slip rubber mats. All models are lightweight and portable for
easy transportation from the bench to
incubator and cold room alike, while
additional platforms may be added for
increased capacity.
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
ixing enzyme and PCR reactions.
M
Staining, blocking and antibody
incubations. Tissue culture.
CS-NOR - Orbital
The CS-NOR includes a 30x30cm
orbital shaking-platform that may be
programmed to perform up to ten 20mm
orbits within each clockwise or anticlockwise shaking cycle. This generates the
swirling motion optimal for aeration of
samples, 0.5 to 5ml in volume, within
multi-well plates, standard dishes and
Petri dishes.
CS-NRC – Reciprocal
The CS-NRC is a 30x30cm reciprocal
shaking-platform incubator that has a linear reciprocating motion which is perfect
for incubation of western blots and initial
mixing of reagents within 96-well microplates during enzyme assays and PCR.
CS-NRK - Rocking
The CS-NRK includes a 30x30cm
rocking platform to provide the perfect
motion and tilt angle to prevent gels
and membranes from drying out during
staining, blocking and antibody incubations.
CW-23 – 2-D/3-D Gyratory
The CW-23 includes a 33x33cm platform whose 3-D gyratory action is ideal
for gentle, foam-free washing of delicate cell lines within tissue culture. An
optional water bath (CW-WB) may be
mounted on the gyratory platform for
nucleic acid hybridisation applications.
• Nucleic acid hybridisation with
water bath option
CW-23
REAGENTS AND CHEMICALS – OVERVIEW
REAGENTS
AND
CHEMICALS
Please see below a synopsis of the reagents and chemicals now available through Cleaver Scientific.
NEW PRODUCTS
‘Just-Add-DNA’ is a ready-to-use PCR MasterMix. Just add water, DNA primers and template
to use!
runSAFE Stains - four non-cytotoxic, non-mutagenic stains produce instant visualisation of DNA
bands upon irradiation with blue light or UV in
agarose or polyacrylamide gels. See safe series
horizontal section page 31.
CleverGEL Instant Agarose Tablets – dissolve
rapidly with no tedious weighing-out to be performed beforehand.
• Supplied in robust,
convenient packaging for
shipping and long-term
storage
HORIZONTAL NUCLEIC ACID ELECTROPHORESIS
CleverGEL
environmentally
friendly agarose
suitable for routine
nucleic acid
electrophoresis. Low
melting point and high
resolution agarose are
also available.
Pre-made TBE and TAE
in powdered and liquid
form. No weighing-out
of individual chemicals
and pH adjustments
once dissolved.
Ready-to-use DNA
markers – stable
for up to 2 years
and available in 6
molecular weight size
ranges: 100-3000bp,
100-1500bp, 501500bp, 100bp-10Kb
and 250bp-25Kb.
DNA Loading Dye
– mix with DNA
samples to monitor
their progress in
electrophoresis runs.
RNase-Free Water
for use in PCR and
northern blotting
techniques.
GelX Disposable Tips
– for completely safe
and contaminationfree removal of DNA
bands from agarose
gels during cloning.
VERTICAL PROTEIN ELECTROPHORESIS
‘Add colour to your PAGE’ with
PINK Plus and BLUE Wide Range
Protein Markers. Supplied prestained in gel loading buffer for
direct loading and stable for up to 2
years at -20°C. 10-175kDa (PINK
Plus) and 10-245kDa (BLUE Wide
Range) sizes.
PAGE Buffers available in powder
sachets and as pre-made stock
solutions for a variety of native and
denaturing protein gel electrophoresis techniques.
• Time-saving: agarose
and buffers in tablet
and powder-sachet
form eliminating tedious
weighing procedures;
ready-made stock solutions
of electrophoresis and
blotting buffers also
available
• Long ‘shelf-life’ DNA and
protein markers
• Membranes and blotabsorbent filter paper
provided to match the most
popular gel sizes
Horizontal Nucleic Acid Electrophoresis136-140
GelX Disposable Gel Excision Tips
Vertical Protein Electrophoresis
141
142-143
Blotting Membranes, Filter Paper
and Staining Solutions
144
‘Just Add DNA’ PCR® MasterMix
145
• Ready-to-use pre-made
PCR MasterMixes and
nucleic acid stains
BLOTTING
Ready-to-use powdered buffers
and stock solutions for classic
Towbin wet electroblotting techniques and high-intensity semi-dry
transfers.
KEY FEATURES:
At Cleaver Scientific our remit is simple: to provide
everything you need for electrophoresis.
Consequently, Cleaver Scientific now offers a select choice
of reagents and chemicals for electrophoresis and molecular
biology techniques. Each Cleaver Scientific reagent is
manufactured to a high standard free of impurities and tested
using the most stringent analytical methods.
Blot-absorbent filter paper in
convenient 10x10cm and 20x20cm
sizes for efficient transfers.
Membrane rolls in 0.24x3m and
0.3x3m (WxL) sizes in PVDF, Nitrocellulose and positively charged
supported nylon for protein and
nucleic acid blotting applications.
Reusable Ponceau S staining
solution for membrane staining and
protein detection following transfer.
RELATED PRODUCTS
Horizontal Gel
systems
PAGES 4-45
Vertical Gel
systems
PAGE 46-71
Blotters
PAGES 72-81
PCR Cabinets
PAGE 128
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136
I REAGENTS & CHEMICALS
REAGENTS & CHEMICALS I 137
Three types
of agarose
available:
Low EEO general
purpose agarose
resolves 100bp to 25Kb
in size
Low Melting Point – for
nucleic acid recovery
– typically resolves
fragments 200bp to
25Kb
Cleaver Scientific now offers a choice selection of chemicals and reagents for electrophoresis and
molecular biology techniques. Each Cleaver Scientific reagent is manufactured to a high a standard
free of impurities and tested using the most stringent analytical methods.
Horizontal
Nucleic Acid
Electrophoresis
CleverGEL – High Grade Agarose for
Nucleic Acid Electrophoresis
Clever for DNA and Clever for the
Environment
CleverGEL is a new environmentally friendly agarose suitable
for routine analysis of nucleic acids using standard electrophoretic procedures. CleverGEL is manufactured by a
process which excludes organic solvents harmful to marine
life, making it far kinder to the environment than conventional agarose.
A low EEO (electroendoosmotic) flow minimises diffusion
so that even the smallest of nucleic acid fragments remains
sharp and tightly resolved, while a high gel strength aids
handling and maintains compatibility with blotting techniques.
CleverGEL is now available in a low melting point form for
nucleic acid recovery and enzymatic applications, as well as
in a high resolution PCR-grade form to resolve very small
nucleic acid fragments 20-800bp in size.
High Resolution – PCR
grade – high resolution,
low background for
analysis of fragments,
20-800bp
TECHNICAL SPECIFICATIONS
Low EEO
Low Melting
Point
CAS
9012-36-6
39346-81-1
39346-81-1
EEO
<0.13
≤0.1
≤0.1
Gelling Point*
36°C±1.5°C
26-30°C
≤33°C
Melting Point*
88°C±1.5°C
≤65°C
≤70°C
Solubility
Clear, colourless
@ 1% [w/v]
solution
Moisture
≤10%
≤10%
≤10%
Gel Strength
>1200 g/cm2
(1% [w/v] Gel)
>200 g/cm2
(1% [w/v] Gel)
≥750 g/cm2
(1.5% [w/v]
Gel)
Yes
Yes
Yes
Nuclease &
Protease Free
High Resolution
Clear, colourless @ 2% [w/v]
solution
*For a 1.5% [w/v] gel
ORDERING INFORMATION
CSL-AG5
Agarose 5g, Low EEO
Low Melting Point
CSL-AG100
Agarose 100g, Low
EEO
CSL-LMA5
Agarose 5g,
LMP
CSL-AG500
Agarose 500g, Low
EEO
CSL-LMA50
Agarose 50g,
LMP
CSL-AG1000
Agarose 1000g, Low
EEO (2x500g bottles)
CSL-LMA100
CSL-AG2000
Agarose 2000g, Low
EEO (4x500g)
CSL-AG5000
Agarose 5000g, Low
EEO (10x500g)
CSL-HRA100
Agarose 100g,
HR
CSL-AG10KG
Agarose 10Kg, Low
EEO (20x500g)
CSL-HRA500
Agarose 500g,
HR
Agarose 100g,
LMP
High Resolution PCR-grade
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
features:
• Each tablet constitutes
0.5g of agarose,
eliminating the need for
tedious, inconvenient and
sometimes inaccurate
weighing procedures
• Faster to prepare: simply
add the required number
of tablets to buffer, and
then heat, dissolve and
pour the gel as usual
• 200 tablets supplied in
total, in 20 foil blister packs
of 10 tablets
• Made from
environmentally friendly
CleverGEL low EEO
agarose (Page 140)
• ISO9001:2008 compliant
• Fast Dissolving
• Consistent Gel Percentage
• No Weighing Necessary
TECHNICAL SPECIFICATIONS
Low EEO
CAS
9012-36-6
EEO
<0.13
Gelling Point*
36°C±1.5°C
Melting Point*
88°C±1.5°C
Solubility
Clear, colourless @ 1% [w/v] solution
Moisture
≤10%
Gel Strength
>1200 g/cm2 (1% [w/v] Gel)
Nuclease & Protease Free
Yes
*For a 1.5% [w/v] gel
ORDERING INFORMATION
CSL-AGTAB
Agarose 100g, Low EEO (200x0.5g
tablets, supplied as 20 blister packs of
10x0.5g tablets)
Typical Applications
For horizontal agarose gels resolving nucleic acids 0.1-10Kb
in size. For industrial and academic laboratories adhering to
rigorous standard operating procedures and protocols
CleverGEL
Instant Agarose
Tablets
Environmentally friendly CleverGEL agarose is now available in tablet form for routine analysis of nucleic acids using
standard electrophoretic procedures. Supplied as 200 tablets
in total, each tablet contains 0.5g of CleverGEL agarose
which eliminates the need to weigh out loose agarose powder. Because each tablet is fast-dissolving all that is required
is to add the desired number of tablets to the buffer, heat,
dissolve and then prepare the gel as normal. CleverGEL
agarose tablets are provided in blister packs of 10, rather
than bulky bottles, for extra convenience and storage.
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FOR MORE INFORMATION
138
I REAGENTS & CHEMICALS
REAGENTS & CHEMICALS I 139
Ready To Use
DNA Markers
ORDERING INFORMATION
Powdered and
Liquid Buffers
Cleaver Scientific supplies TBE and TAE in powder and liquid
form. Both TBE and TAE are widely used to separate nucleic
acids by horizontal gel electrophoresis, and occasionally in
vertical polyacrylamide gels. TBE has a higher buffering capacity than TAE, which is used for faster separations of linear
double-stranded DNA.
Cleaver Scientific dry TBE is supplied in packs of 10 powder
sachets to maintain shelf life. Each buffer sachet may be
opened as required and reconstituted in distilled water to
make 1 litre of working solution.
Buffers are also provided as ready-made 50x TAE and 10x
TBE solutions in 1 and 5 litre volumes. These are ideal for
laboratories running horizontal nucleic acid gels on a daily
basis that require off-the-shelf working stock solutions.
Typical Applications
Pre-made concentrated buffers for DNA and RNA
Electrophoresis
ORDERING INFORMATION
CSL-TBEP
Powdered Tris-Borate-EDTA Running Buffer –
10 sachets (1 litre / pack)
TBE10X1L
Cleaver Buffer Tris-Borate-EDTA Running
Buffer, 10 x 1L
TBE10X5
Cleaver Buffer Tris-Borate-EDTA Running
Buffer, 10 x 5L
TAE50X1L
Cleaver Buffer Tris-Acetate-EDTA Running
Buffer, 50 x 1L
TAE50X5L
Cleaver Buffer Tris-Acetate-EDTA Running
Buffer, 50 x 5L
TECHNICAL SPECIFICATIONS
TAE
TBE
Each 50x solution contains: Tris-base (2.0M final
stock concentration); glacial acetic acid (1.0M);
EDTA, pH 8.0 (0.05M); followed by distilled water
to 1L
Each 10x solution / powder contains: Tris-base
(0.112M final stock concentration); boric acid
(0.112M); EDTA, pH 8.0 (0.02M); followed by
distilled water to 1L
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FOR MORE INFORMATION
CSL-MDNA-100BPH
100bp DNA ladder, 100-3000bp, 1x500µl vial
CSL-MDNA-100BP
100bp DNA ladder, 100-1500bp, 1x500µl vial
CSL-MDNA-1KB
1Kb DNA ladder, 250bp-10Kb, 1x500µl vial
CSL-MDNA-50BP
50bp DNA ladder, 50-1500bp, 1x500µl vial
CSL-MDNA-BR
Broad Range DNA ladder, 100bp-10Kb, 1x
500µl vial
CSL-MDNA-HR
High Range DNA ladder, 250bp-25Kb,
1x500µl vial
Pre-made and containing loading dye for immediate use,
Cleaver Scientific’s ready-to-use (RTU) DNA markers are
specially formulated to run accurately and produce sharp,
well defined ladders. Available in six molecular weight ranges and composed of discrete marker fragments isolated from
restriction-digested proprietary plasmids, each DNA marker
will remain stable for up to 6 months at room temperature
and 12 months if kept in the fridge at 4°C. All Cleaver Scientific RTU markers contain high intensity reference bands
and may be used to perform size comparisons with DNA
molecules ranging from the smallest of PCR fragments to
large, linearised cosmid vectors.
TECHNICAL SPECIFICATIONS
Product Code
CSL-MDNA100BPH
CSL-MDNA100BP
CSL-MDNA-1KB
CSL-MDNA-50BP CSL-MDNA-BR
CSL-MDNA-HR
Size Range
100-3000bp
100-1500bp
250bp-10Kb
50-1500bp
100bp-10Kb
250bp-25Kb
Number of Bands
12
11
13
17
19
14
Reference Bands
500, 1500bp
500, 1500bp
1Kb, 3Kb
200, 500bp
500bp, 1.5 & 3Kb 1Kb, 3Kb
Package Concentration
54µg/500µl vial
50µg/500µl vial
50µg/500µl vial
56µg/500µl vial
86µg/500µl vial
Storage
Recommended loading
Volume
5µl/well
5µl/well
Tracking Dyes
Source
52µg/500µl vial
6 months at 25°C, 12 months at 4°C & 24 months at - 20°C
5µl/well
5µl/well
5µl/well
5µl/well
Orange G, Xylene Cyanol FF, Bromophenol Blue
Proprietary plasmids and PCR fragments phenol-extracted following restriction digestion and dissolved in
10mM Tris-HCl (pH 8.0) and 10mM EDTA.
Typical Applications
Size determination of DNA and RNA bands on gels
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140
I REAGENTS & CHEMICALS
DNA Loading
Dye
DNA loading dye contains 10x bromophenol blue, the
standard tracking dye for electrophoresis. The charge-tomass ratio of bromophenol blue allows it to co-migrate with
smaller molecules within agarose and PAGE gels (e.g. at
300bp in a standard 1% agarose, TBE gel) which, with its
conspicuous dark blue colour, makes it the perfect tracking
dye to monitor the progress of electrophoresis runs. DNA
loading dye is supplied in 1ml volumes for easy handling.
REAGENTS & CHEMICALS I 141
RNase-Free
Water
DEPC-treated to eliminate enzyme activity and then autoclaved, this sterile highly purified water product is perfect for
use in PCR and Northern blotting techniques. RNase-Free
water is available either as a single 250ml bottle or in fifty
5ml aliquots to prevent cross-contamination.
ORDERING INFORMATION
RFW250
RNase-Free Water, 1x250ml
RFW50X5
RNase-Free Water, 50x5ml
ORDERING INFORMATION
CSL-LOADDYE
10x Bromophenol Blue Loading Dye, 1ml
CSL-LOADDYE10
10x Bromophenol Blue Loading Dye, 10ml
Orange G
Loading Dye
Orange G Loading dye 1x (with ficoll) Used as a marker in
PAGE and Agarose electrophoresis of DNA, as it migrates
through the gel consistently with smaller DNA fragments.
Contains sucrose and Xylene Cyanol. Used as a 1x solution.
ORDERING INFORMATION
CSL-ORANGEDYE
Orange G Loading Dye, 1ml
BP Grade Ultra
Pure Water
GeIX
Disposable Gel
Excision Tips
Purified water (18 mega Ohms) for use with sensitive experimental procedures often needs verifying as pyrogen free,
this is done using the LAL test or Limulus (Horseshoe crab)
amoebocyte lysate assay. The LAL test is extremely sensitive
to endotoxins which are the result of bacterial lysis.
Widely acclaimed as an excellent tool for gene cloning,
Cleaver Scientific GelX disposable gel excision tips offer a
unique and convenient way to excise nucleic acid and
protein bands from gels. GelX tips allow completely safe
and contamination free removal of the gel band of interest
without the need to handle potentially dangerous scalpels.
The gel band is neatly extracted and contained in the tip,
when it is then dispensed into a tube for further analysis.
Once used, the tip is ejected for disposal.
BP Grade Sterile Water has endotoxins removed by electrostatic filtration at the final purification stage prior to
autoclaving. The LAL tested water conforms to the standard having less than <0.25EU/ml to ensure the water is of
pre-requisite quality. This product is therefore pyrogen free.
CFU>0 WFi compatible.
LAL (Limulus Amoebocyte Lysate Assay)
• Eliminates scalpel damage to transilluminator or gel tray
• Designed for removing bands from agarose and
protein gels
ORDERING INFORMATION
UPW1000
Typical Applications
Useful reagents for PCR, Electrophoresis and Blotting
BP Grade Sterile Water, 1000ml
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ORDERING INFORMATION
• One-handed operation
CSL-GELX4
4mm x 1mm, Gel Cutting Tips, 250/ bag
• Push button gel and tip release mechanism
CSL-GELX4RACK
4mm x 1mm, Gel Cutting Tips, 5 racks of 48
• Fits standard 1000μl pipettes
CSL-GELX6.5
6.5mm x 1mm, Gel Cutting Tips, 250/ bag
• Autoclavable, and DNase and RNase free
CSL-GELX6.5RACK
6.5mm x 1mm, Gel Cutting Tips, 5 racks of 48
• Sterile versions also available
Typical Applications
Cutting out and purification of DNA bands for cloning
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142
I REAGENTS & CHEMICALS
REAGENTS & CHEMICALS I 143
features:
• Convenient, pre-made
stock solution or powder
– just dilute or dissolve
as necessary with water
PINK Plus
• Save time & trouble
– no weighing, pH
adjustment or need
to stock individual
compounds
BLUE Wide
• Long shelf-life
Vertical Protein
Electrophoresis
PINK Plus and BLUE Wide Range
Protein Markers
• Consistency assured
– rigorous QC for
reproducible separations
features:
• Standard (10-175kDa) and wide (10-245kDa)
molecular weight ranges available
Adding colour to your PAGE
• Coloured reference bands for easy
identification in gels and blots
Stable for up to 2 years if stored at -20°C and supplied prestained in gel loading buffer for direct loading, Cleaver Scientific PINK Plus and BLUE Wide Range recombinant protein
markers are perfect for SDS-PAGE applications. Sizes range
from 10-175kDa for PINK Plus and 10-245kDa for BLUE
Wide Range, making both markers suitable for accurate
molecular weight determination of most cellular proteins.
• Stable for 2 years at -20°C
Each marker is covalently bound to a pink or blue colour
chromaphore to produce a ladder of evenly interspersed
bands of uniform intensity. Coloured reference bands serve
as visual indicators of electrophoresis run progression and
the efficiency of western transfer onto membranes following
SDS-PAGE. Both PINK Plus and BLUE Wide Range markers
can be detected at volumes as low as 2.5µl per well.
TECHNICAL SPECIFICATIONS
Product
Code
CSL-PPL
CSL-BBL
Size Range
10-175kDa
10-245kDa
Number of
Bands
11
12
Reference
Bands
10, 40 and 90kDa blue
25 & 75kDa; green & red
Contents
Max. 2.2mg total protein
in 15% (v/v) glycerol, 2%
SDS, 20mM Tris pH 7.5,
1mM 2-ME and 3.6M Urea
Max. 2.4mg total protein
in 15% (v/v) glycerol, 2%
SDS, 20mM Tris pH 7.5,
1mM 2-ME and 3.6M Urea
500µl
500µl
Volume
Supplied
Storage
Stable for up to 2 weeks at 25°C,
3 months at 4°C & 24 months at -20°C
Loading
Volume
2.5-5µl/well
CSL-PPL
CSL Pink Plus Prestained Protein Ladder,
10-175kDa, with 10, 40 & 90kDa reference
bands, 1x 500μL vial.
Applications
CSL-BBL
CSL BLUE Wide Range Prestained Protein
Ladder, 10-245kDa, with 25 & 75kDa
reference bands, 1x 500μL vial.
ORDERING INFORMATION
Typical Applications
Size determination of denatured proteins on
electrophoresis gels
Number of
Source
100-200
Recombinant proteins, various sources
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FOR MORE INFORMATION
TECHNICAL SPECIFICATIONS
Powder
Buffer
Composition
Applications
TrisGlycineSDS
Each litre of 1x working
solution contains: Tris-base
(25mM); glycine (192mM);
SDS, 0.1% (w/v); followed
by distilled water. Working
solution pH = 8.3.
Denaturing SDS-PAGE for
most cellular proteins, 10200kDa in size
TrisGlycine
Each litre of 1x working
solution contains: Tris-base
(25mM); glycine (192mM);
followed by distilled water.
Working solution pH = 8.3.
Native PAGE
TrisTricineSDS
Each litre of 1x working
solution contains: Tris-base
(0.1M); tricine, (0.1M);
SDS, 0.1% (w/v); followed
by distilled water. Working
solution pH = 8.25.
Denaturing SDS-PAGE, with
greater resolving power
for small proteins 2-20kDa
in size
MOPSSDS
Denaturing SDS-PAGE for
Each litre of 1x working
solution contains: MOPS
medium- to large-sized
(50mM); Tris Base (50mM); proteins
SDS, 0.1% (w/v); EDTA
(1mM); followed by distilled
water. Working solution pH
= 7.7.
MES-SDS
Each litre of 1x working
solution contains: MES
(50mM final stock
concentration); Tris Base
(50mM); SDS, 0.1% (w/v);
EDTA (1mM); followed by
distilled water. Working
solution pH = 7.3.
Denaturing SDS-PAGE for
small- to medium-sized
proteins; faster than MOPS
Typical Applications
Used with protein gels in vertical electrophoresis systems
Page Buffers
Five buffers are available in powder sachets for a range of
native and denaturing protein gel electrophoresis techniques.
Each powder sachet, which is supplied as a 10-pack, may be
reconstituted to make 1 litre of working solution. Running
buffers are also available in 1 litre and 5 litre volumes as
ready-made 10x Tris-Glycine and 10x Tris-Glycine-SDS
solutions.
ORDERING INFORMATION
Powder Buffers
CSL-TGSDSP
Powdered Tris-Glycine-SDS Running buffer
- 10 Sachets (10 litres/pk)
CSL-TGP
Powdered Tris-Glycine Running buffer
- 10 Sachets (10 litres/pk)
CSL-TTSDSP
Powdered Tris-Tricine-SDS Running buffer
- 10 Sachets (10 litres/pk)
CSL-MSDSP
Powdered MOPS-SDS buffer Running buffer
- 10 Sachets (10 litres/pk)
CSL-MESDSP
Powdered MES-SDS buffer Running buffer
- 10 Sachets (10 litres/pk)
Liquid Buffers
TG10X1L
Cleaver Buffer Tris-Glycine 10 x 1litre
TG10X5L
Cleaver Buffer Tris-Glycine 10 x 5litre
TG-SDS10X1L
Cleaver Buffer Tris-Glycine SDS 10 x 1litre
TG-SDS10X5L
Cleaver Buffer Tris-Glycine SDS 10 x 5litre
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144
I REAGENTS & CHEMICALS
REAGENTS & CHEMICALS I 145
Blotting Membrane Rolls
Blot-Absorbent Filter Paper
Supplied in 0.24x3m and 0.3x3m (w x l) sizes, allowing
them to be cut to match specific gel formats, these membrane rolls are suitable for transfer of proteins and nucleic
acids from polyacrylamide and agarose gels. Offered in 0.2
and 0.45µm pore sizes.
Cleaver Scientific blot-absorbent filter paper is supplied in
packs of 50 and in sizes of 10x10cm and 20x20cm. Its 1-mmthick texture and high buffer retention properties, being able
to absorb twice its own weight in buffer, allow it to exert the
gel-membrane compression needed for efficient transfers.
ORDERING INFORMATION
ORDERING INFORMATION
Code
Description
CSL-BP1010
Blot-Absorbent Filter paper, 10x10cm, pack of 50
CSL-RNC45
Nitrocellulose roll, 0.3x3m (w x l)
CSL-BP2020
Blot-Absorbent Filter paper, 20x20cm, pack of 50
CSL-RNC2
Nitrocellulose roll, 0.3x3m (w x l)
CSL-RNY45
Positively charged supported nylon, 0.24x3m (w x l)
CSL-RNY2
Positively charged supported nylon, 0.24x3m (w x l)
TECHNICAL SPECIFICATIONS
Code
Pore Size
Recommended Applications
CSL-RNC45
0.45µm
For most analytical blotting procedures,
including protein, single-stranded DNA
& RNA; compatible with colorimetric,
radiolabelled, chemiluminescent and
fluorescent detection and staining
methods
CSL-RNC2
0.2µm
For small proteins <15kDa
CSL-RNY45
0.45µm
CSL-RNY2
0.2µm
For Southern blots and transfer of nucleic
acids from agarose and acrylamide gels;
rapid alkaline Southern transfer
10 Pre-cut PVDF 28 x28 cm 0.22 µm
CSL-PVDF0.45S
10 Pre-cut PVDF 28 x28 cm 0.45 µm CSL-PVDF0.45R
Roll PVDF 28 cm x 3 m, 0.45 µm CSL-PVDF0.22R
Roll PVDF 28 cm x 3 m, 0.22 µm Typical Applications
Membranes, stains and reagents for
blotting
• No agarose gel staining and destaining
necessary
Ponceau S staining solution is reusable and available in a
convenient 500ml volume for membrane staining and early
protein detection following transfer before western blotting.
Ponceau S may also be supplied a powder staining kit for
long-term storage.
• Contains Thermus Aquaticus Taq DNA
polymerase - expressed and purified from
recombinant E. coli – with 5’-3’ polymerase
and exonuclease activities
ORDERING INFORMATION
CSL-PSS
Ponceau S staining solution (500ml)
CSL-PSB
Ponceau S staining solution powder staining kit
(makes 2000ml)
• As sensitive as ethidium bromide, but
without the mutagenicity and associated
environmental and disposal hazards
• Supplied in two 1.25ml vials to make up to
100 PCR reactions, each of 50µl final volume
• Ideal for use in the GTC96S thermal cycler
(Pg 123)
ORDERING INFORMATION
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FOR MORE INFORMATION
Ligation compatible: ‘Just-Add-DNA’ has no adverse effect on ligation of
PCR products following PCR. Recombinant plasmids containing identical
insert after amplification with standard PCR (1) and ‘Just-Add-DNA’ (2)
master mixes. Stable for at least 3 months at room temperature.
• Lack of 3’-5’ exonuclease and proofreading
activity makes ‘Just-Add-DNA’ useful for PCRbased site-directed mutagenesis applications
• Long shelf-life: stable for 3 months at room
temperature and up to a year at -20°C
ORDERING INFORMATION
Description
• Just mix with an equal volume of DNA and
primer solution, perform PCR and then run the
resultant products straight out on an agarose
gel without prior mixing with loading dye
• UV- and blue-light fluorescent; perfect for
runVIEW (Pg 28)
Used in Western Blotting of Proteins and for use in Hybridisation Tehcniques. PDVF with nitrocellulose and nylon
membranes are available for different application needs.
A protein’s properties (i.e., charge, hydrophobicity, etc.)
affects its ability to bind to membrane surfaces. Finding the
optimal membrane may require experimenting with your
specific protein on different materials. We supply PVDF
membrane in sheet form and as a 3M role which can be cut
to size to fit your particular need.
CSL-PVDF0.22S
• Ready-to-use, pre-mixed 2x PCR® MasterMix
that contains Taq DNA Polymerase, PCR
buffer, dNTPs, and fluorescent stain and
loading dye for electrophoresis post-PCR
Ponceau S
PVDF Sheets &
Membrane Rolls
Code
features:
CSL-JADNA
‘Just-Add-DNA’ MasterMix, 2x 1.25ml vials,
sufficient for 100 reactions
CSL-JADNA-10
‘Just-Add-DNA’ MasterMix, 20x 1.25ml vials,
sufficient for 1000 reactions
CSL-RV-JA-DNA
CSL-runVIEW PCR kit for users already with
thermal cycler; incl. CSL-RVMSCHOICETRIO
plus CSL-JADNA-10
CSL-GTC96-JA-DNA
CSL-GTC96S PCR kit for users already with
runVIEW or an electrophoresis system; incl.
CSL-GTC96S plus CSL-JADNA-10
CSL-GTC96-RV-JADNA
Complete PCR/real-time DNA electrophoresis
workstation; incl. CSL-GTC96S & CSLRVMSCHOICETRIO, plus CSL-JADNA-10
Typical Applications
All in one PCR mix plus electrophoresis safe stain for direct
loading onto gels
Fluorescence excitation and emission maxima of the fluorescent stain within ‘Just-Add-DNA’. Excitation is maximal around 490nm with a secondary
peak at 490nm. Fluorescence emission is maximal at around 550nm.
‘Just Add DNA’
PCR® MasterMiX
‘Just-Add-DNA’ is a ready-to-use PCR® MasterMix that
requires only water, DNA primers and template to execute
primer elongation or the DNA polymerisation step of PCR®.
With ‘Just-Add-DNA’ MasterMix time-consuming MasterMix
preparation is rendered unnecessary, as Taq DNA polymerase,
PCR buffer, deoxynucleotides (dNTPs), gel-loading dye and
fluorescent stain are already premixed at 2x concentration,
and may be dispensed in 25µl aliquots to an identical volume
of DNA template and primer solution. Once mixed, PCR may
be performed as usual and the resultant PCR products then
checked following transfer from the thermal cycler block to
an agarose gel, which is visualised on a UV transilluminator or
by using runVIEW - our award-winning blue-light real-time
horizontal gel electrophoresis system (Pg 28 ).
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OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Complimentary
products
COMPLIMENTARY PRODUCTS OVERVIEW
In our range of complimentary products are our radiation protection products, glove boxes
and balances.
Radiation Safety Products
Cleaver Scientific provide radiation protection
shielding for use in Laboratories. Three
types are available for shielding against
Beta emitters, Gamma emitters and for dual
blocking.
Beta Protection Products
The accepted standard for shielding beta
emissions of 32P, 35S, 3H and 14C is 10mm
thick optical acrylic which is an effective
and ideal material for the construction of
beta radiation protection equipment. Its
properties provide easy fabrication and
manufacturability into a wide range of
products.
Gamma Protection Products
Now readily available is a lead containing
acrylic sheet which offers complete visibility
of stored gamma emitting radioisotopes
unlike the non transparent solid lead
shielding which until recently has been used
as protection against isotopes such as 125I,
133
Xe, 57Co, 99mTe, 123I and 67Ga.
This transparent shielding has a lead content
of 30% which is introduced as an organo
lead salt.
The CSL range of products using 12mm thick
lead acrylic, 0.5mm thick lead equivalent,
attenuates gamma emissions from 125I and
of lesser energy. The 35mm thick lead
acrylic, 1.7mm thick lead equivalent, can
be purchased for use with more energetic
isotopes of Iodine.
Glove Boxes
Cleaver Scientific Glove Boxes are
manufactured from Poly carbonate and
provide a contained area for a variety of
isolation procedures.
These boxes are ideal for use as isolation
chambers for potentially harmful samples
and substances. In addition they can also
be used to maintain consistent atmospheres
and conditions around sensitive samples
including:- moisture / humidity controlled
or free atmosphere, inert gas (nitrogen)
atmosphere, anaerobic atmosphere and
oxygen rich atmosphere.
Cleaver Scientific Glove Boxes are available in
four sizes but are also customizable for your
specific requirements.
Radiation Safety
148-151
Glove Boxes
152
Balances153
Balances
Cleaver Scientific offer the full range of
Adams balances and weighing instruments
of a variety of types including portable,
precision, analytical, compact and triple
beam.
RELATED PRODUCTS
Reagents
PAGES 134-135
Rockers and Shakers PAGE 132
Thermal cyclers
PAGE 123
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148
I RADIATION SAFETY
RADIATION SAFETY I 149
ORDERING INFORMATION
BETA
GAMMA
BOX SIZE
CSR-S1
CSR-S1G
Small Fixed 15° Angle - Flat Base 30 x
45cm, Base 30 x 30cm
CSR-S1T
CSR-S1TG
Small Fixed 15° Angle - Curved Base 30
x 45cm
CSR-S2
CSR-S2G
Large Fixed 15° Angle - Flat Base 35 x
53cm, Base 35 x 30cm
CSR-S2T
CSR-S2TG
Large Fixed 15° Angle - Curved Base 35
x 53cm
CSR-S1O
CSR-S1OG
Small Fixed 45° Angle - Flat Base 30 x
45cm, Base 30 x 30cm
CSR-S1OT
CSR-S1OTG
Small Fixed 45° Angle - Curved Base 30
x 45cm
CSR-S2O
CSR-S2OG
Large Fixed 45° Angle - Flat Base 35 x
60cm, Base 30 x 30cm
CSR-S2OT
CSR-S2OTG
Large Fixed 45° Angle - Curved Base 35
x 60cm
CSR-S3
CSR-S3G
3-Sided Front 46 x 50cm, Sides 30 x
50cm
CSR-S4
CSR-S4G
Hourglass Shield - Flat Base 30 x 45cm,
Base 30 x 30cm
CSR-S4T
CSR-S4TG
Hourglass Shield - Curved Base 30 x
45cm
CSR-SFLEXI
CSR-SFLEXIG
Shield - Adjustable 35 x 54 or 54 x 35cm
CSR-SF
CSR-SFG
Base Plate 45 x 41cm
CSR-SDUO
DuoShield - Curved Base, Beta/Gamma
30 x 45cm
CSR-SFLEXITG
Shield - Adjustable, 35 x 54 or 54 x
35cm, 35mm thick
CSR-S1, CSR-S2
CSR-S1T, CSR-S2T
CSR-S1O, CSR-S2O
CSR-S3
CSR-S4, CSR-S4T
CSR-SFLEXI
ORDERING INFORMATION
Radiation Safety
TRAYS AND LINERS
Radiation
General
BioHazard
APET liners,
Hazard tray
purpose tray,
tray, White
pk 25
Yellow
White
Tray size
46 x 26cm
CSR-TY1
CSR-TO1
CSR-TW1
CSL-TL1
54 x 34cm
CSR-TY2
CSR-TO2
CSR-TW2
CSL-TL2
57 x 54cm
CSR-TY3
CSR-TO3
CSR-TW3
CSL-TL3
68 x 54cm
CSR-TY4
CSR-TO4
CSR-TW4
CSL-TL4
70 x 46cm
CSR-TY5
CSR-TO5
CSR-TW5
CSL-TL5
113 x 54cm
CSR-TY6
CSR-TO6
CSR-TW6
CSL-TL6
CSR-SF
CSR-TY4
Available in standard 10mm beta-protecting acrylic, 12mm gamma-attenuating lead acrylic and also as duo
shielding for protection against both
types of emission, Cleaver Scientific’s
comprehensive range of Radiation
Safety Products comprises a large
selection of shields, boxes, waste bins,
trays, plus assorted accessories and
cabinets.
ORDERING INFORMATION
CABINETS
BETA
GAMMA
BOX SIZE
CSR-CAB
CSR-CABG
Beta Work Cabinet, 49 x 55 x 37cm
CSR-TW1
CSR-CAB
Shields
Supplied in small, medium and large
sizes and with curved base 15cmdeep for use with safety trays or flat
30x30cm base for under-the-bench
protection. A range of angles offers
increased manoeuvrability, while clear
optical acrylic aids visualisation.
Trays and Liners
Available in general purpose, biohazard
and radiation safety formats, spilltrays
provide a re-usable work area with the
added benefit of safe containment of
spillages and lab bench protection. All
trays supplied with one free liner when
purchased. Additional environmentally
Cabinets
Cleaver Scientific cabinets provide a
convenient area to carry out work with
beta and gamma emitting isotopes
with complete all round protection. Each cabinet’s 49 x 55 x 37cm
dimensions offer a large working area
without impeding vision, either in a
standing or seated position.
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Protect users of radioactive isotopes from exposure to radiation. Allow visualisation of the sample
and work area without exposure.
150
I RADIATION SAFETY
RADIATION SAFETY I 151
ORDERING INFORMATION
BETA
GAMMA
BOX SIZE
ORDERING INFORMATION
BETA
BINS
GAMMA
BOX SIZE
CSR-B1
CSR-B1G
CSRB2TIP
2L, use with bag CSR-BAG1 13 x 13x
CSR-B2TIPG
13cm
-
Mini Box Insert - 16 x 1.5ml tubes
CSR-R50
-
Maxi Box Insert - 8 x 50ml Centrifuge
tubes
CSRB2MCTIP
CSRB2MCTIPG
2L, use with bag CSR-BAG1 13 x 13x 13cm
CSR-R0.5
-
Mini Box Insert - 20 x 0.5ml tubes
CSR-B3
CSR-B3G
3.3L, use with bag CSR-BAG1 15 x 15 x
15cm
CSR-R3F
-
Maxi Box Insert - 3 x Falcon tubes, 8 x
1.5ml tubes
CSR-B10
CSR-B10G
10L, use with bag CSR-BAG2 25 x 20 x
20cm
CSR-R1.5L
-
Midi Box Insert - 32 x 1.5ml Eppendorf
tubes
CSR-B15
CSR-B15G
15L, use with bag CSR-BAG2 29.5 x 21.5
x 23.5cm
CSR-R2F
-
Maxi Box Insert - 2 x Falcon tubes, 8 x
1.5ml tubes
Large 5L, use with bag CSR-BAG2 33 x 13
x 13cm
CSR-R0.5L
-
Midi Box Insert - 40 x 0.5ml Eppendorf
tubes
CSRB5TIP
CSR-B6.6
INSERTS
-
1L, use with bag CSR-BAG1 13 x 10 x 8cm
CSR-R1.5
CSR-B20
CSR-B20G
20L, use with bag CSR-BAG5 38 x 21.5 x
23.5cm
CSR-R20
-
Maxi Box Insert - 8 x 20ml Scintillation
vials
CSR-B53
CSR-B53G
53L, use with bag CSR-BAG5 40 x 49 x
27cm
CSR-RDUO
-
Midi Box Insert - 16 x 1.5 and 16 x
0.5ml tubes
CSR-R5
-
Maxi Box Insert - 15 x 5ml Scintillation
vials
CSR-R2
-
Midi Box Insert - 32 x 2ml Cryotubes
CSR-RU
-
Maxi Box Insert - 8 x Universals
-
Maxi Box Insert - 15 x 15ml Centrifuge
tubes
CSR-B47
-
47L with Wheels, use with bag CSR-BAG6
58 x 28.5 x 27cm
CSR-B122
-
122L with Wheels, use with bag CSR-BAG6
74 x 41 x 41cm
BAGS
CSR-R15
RBAG1
Printed Yellow Tinted Bags, 100/pack 200x150x380mm
RBAG2
Printed Yellow Tinted Bags, 100/pack 280x100x610mm
RBAG3
Printed Yellow Tinted Bags, 100/pack 280x280x760mm
RBAG4
Printed Yellow Tinted Bags, 250/roll
CSR-STORE
CSR-B15
610x915mm
CSR-PB2
CSR-PB2G
Pipette Shield - Biohit PS1000 Beta
CSR-PB1
CSR-PB1G
Pipette Shield - Biohit PS200 Beta
CSR-PB3
CSR-PB3G
Pipette Shield - Biohit PS5000 Beta
CSR-PG3
CSR-PG3G
Pipette Shield - Gilson P1000 Beta
RBAG5
Unprinted Clear Bags, 100/pack
200x405mm
RBAG6
Unprinted Clear Bags, 100/pack
255x305mm
RBAG7
Unprinted Clear Bags, 100/pack
305x455mm
CSR-PG1
CSR-PG1G
Pipette Shield - Gilson P20/100 Beta
RBAG8
Unprinted Clear Bags, 100/pack
305x505mm
CSR-PG2
CSR-PG2G
Pipette Shield - Gilson P200 Beta
RBAG9
Unprinted Clear Bags, 100/pack
355x405mm
RBAG10
Unprinted Clear Bags, 100/pack
610x915mm
Inserts
LABELS
CSR-LABS
-
Radiation Labels, pk/25 25x25mm
CSL-LABL
-
Radiation Labels, pk/25 50x50mm
-
Radiation Tape, 1 Roll 25mm x 66m
Radiation Safety
TAPE
CSR-B0.4G
Mini 5.5 x 8.5 x 8.5cm
CSR-B0.8
CSR-B0.8G
Midi 6 x 16.5 x 8.5cm
CSR-B6.5
CSR-B6.5G
Maxi 14 x 28 x 16.5cm
CSR-B3.5
CSR-B3.5G
Box for four way racks 14 x 17.5 x 15cm
CSR-B8
CSR-B8G
Transport Box 7.5 x 29.5 x 38cm
CSRBDUO
CSRBDUOG
Duo Box 7 x 10 x6cm
CSRBLOCK
CSRBLOCKG
Block for 4 x 1.5ml Eppendorf tubes 5 x 3.5
x 14cm
CSRBLOCKL
CSRBLOCKLG
Cover for B4 1 x 3.5 x 14cm
CSRSTORE
CSRSTOREG
Beta-Storage/Transport Block 7 x 15 x 12cm
CSR-COV CSR-COVG
Pipette Guard
ACCESSORIES
GAMMA
CSR-B0.4
CSR-B122
ORDERING INFORMATION
BETA
BOXES
CSR-B8
CSRRADTAPE
CSR-B0.4
CSR-B0.8
CSR-B2TIP
Carboy Cover 59 x 38 x 38cm
Waste Bins, Bags
and Inserts
Cleaver Scientific storage boxes are
manufactured with hinged lids and
accommodate interchangeable inserts
that hold microtubes, centrifuge tubes,
scintillation vials, universals, cryotubes
and falcon tubes. Also supplied is our
range of floor-standing and benchtop
bins with anti-slip feet and hinged
lids. These serve as an ideal solution
for short-term storage of radioactive
waste or radioisotopes. Both the Beta
and Gamma storage bins are available
in five sizes, while the two largest bin
models have wheels for easy transportation. Optional heavy duty drawstring
bags may also be purchased.
Accessories
Other accessories available include
pipette guards, radiation tape and
warning signs and labels.
Typical Applications
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
Storage of radioactive samples in various sized visible boxes with different types of inserts for different sized vials and tubes.
Disposal of radioactive waste.
152
I RADIATION SAFETY
RADIATION SAFETY I 153
features:
• Available in 4 sizes with or without air locks:
• Standard 2 port 24x24x24” (60x60x60cm)
• Standard 2 port 36x24x24” (90x60x60cm)
• Standard 2 port 48x24x24” (120x60x60cm)
• Standard 2 port 60x24x24” (150x60x60cm)
Adam® Equipment Balances
Cleaver Scientific is delighted to announce its distributor partnership with Adam® Equipment Ltd. Adam® Equipment Ltd.
has been manufacturing high quality balances from the UK since 1972, for applications and disciplines that range from
education and agriculture to laboratory research, health care and veterinary medicine. A synopsis of Adam® Equipment
Laboratory Balances offered by Cleaver Scientific is shown below, although balances, scales, moisture analysers and mass
measuring equipment for other applications are also available. For further information, or to receive an Adam® Equipment
brochure or a quotation, please do not hesitate to contact us.
• Provides a barrier between the user and
potential contaminants
Gloveboxes
Cleaver Scientific glove boxes are available in four sizes and
provide an ideal cost-effective solution for those scientists
or users performing functions in which containment is
paramount. A robust construction from non-reactive
polycarbonate allows each glove box to be used with inert
gases such as helium, nitrogen and argon, for procedures
requiring exclusion of atmospheric oxygen and moisture.
Each box, which includes hermetically-sealed gloves and
a side panel as standard, provides a safe barrier between
the worker and any potential contaminant. Glove boxes
may also be supplied with airlocks, and are customisable in
various shapes and sizes to suit different work environments,
applications and spaces. Shelving and pipette holder options
are also available.
NOW Introducing the
Combi Box
The Cleaver Scientific Combi Box combines the benefit of
UV sterilisation with the fully sealed and enclosed area of a
glove box. Four 15W UV-C bulbs may be timer controlled
for up to 30 minutes, or indefinitely, to decontaminate
equipment and the work surface before and after use.
A side panel with safety interlock switches immediately
disconnects the UV source to facilitate easy placement and
removal of samples and packages, while a 15W white light
bulb illuminates the entire work surface to provide excellent
visibility. Two hermetically sealed non-UV transparent
gloves afford the user total manoeuvrability and dexterity
when handling equipment, samples and packages within
the contained environment of the glove box, plus additional
protection when working under the UV source.
• Hermetically sealed gauntlets permit safe
handling within a fully enclosed containment
area
• Side panel allows samples and packages to be
placed in and removed from the work area
safely and easily
• Non-reactive polycarbonate suitable for use
with gases such as helium, nitrogen and
argon to allow work with samples sensitive to
moisture or oxidation
• Customisable in different shapes and sizes to
suit a variety of work settings, applications
and spaces
• Antechamber, lead acrylic, shelving and
pipette holder options available
ORDERING INFORMATION
Part Number
Description
CSL-GB24
Standard 2 port 24x24x24” (60x60x60cm)
CSL-GB24-A
CSL-GB24 with Air-Lock
CSL-GB36
Standard 2 port 36x24x24” (90x60x60cm)
CSL-GB36-A
CSL-GB36 with Air-Lock
CSL-GB48
Standard 2 port 48x24x24” (120x60x60cm)
CSL-GB48-A
CSL-GB48 with Air-Lock
CSL-GB60
Standard 2 port 60x24x24” (150x60x60cm)
CSL-GB60-A
CSL-GB60 with Air-Lock
ORDERING INFORMATION
Part Number
Description
CSL-COMBIBOX
Combination Glove box with 2 ports - 240VAC
CSL-COMBIBOX-A CSL-COMBIBOX with Air-Lock - 240VAC
Use $ for 120VAC versions; e.g CSL-COMBIBOX$
Laboratory Balances & Scales Synopsis
Product
Capacity /
Readability
Nimbus Precision
balances
(Internal calibration)
80g -210g/0.0001g
220g - 420g/0.001g
1020g - 4600g/0.01g
Nimbus Precison
balances
(External calibration)
80g - 210g/0.0001g
220g - 420g /0.001g
2600g - 4600g/0.01g
4200g - 22000g/0.1g
Eclipse Precision
balances
(External calibration)
100g - 310g/0.0001g
220g - 1620g/0.001g
1600g - 6200g/0.01g
4200g - 32000g/0.1g
Eclipse Precision
balances
(Internal calibration)
Applications
Overview
• Weighing
• Net / Total • Check weighing
• Percentage weighing
• Weight accumulation
• Parts counting
• Animal / dynamic weighing
• Density determination
• Below balance weighing
For laboratories, schools, industry and
commercial users
100g - 310g/0.0001g
220g - 1620g/0.001g
1600g - 6200g/0.01g
• Weighing • Parts counting
• Percentage weighing • Check
weighing • Density determination
• Below balance weighing
For those user requiring a high precision
top-loading balance that is portable and
easy to use
Highland™ Portable
Precision Balances
120g-3kg /
0.001g-0.1g
• Weighing • Percentage weighing
• Parts counting • Below balance
weighing • Weight accumulation
For basic laboratory use, field work and
various industrial applications
Core™ Compact
Portable Balances
0.01g-1g /
200g-5000g
• Weighing • Below balance
weighing • Weight accumulation
For simple operation and economy
Dune™ Compact
Balances
200g-5000g /
0.1g-2g
• Weighing
For quick and easy weighing
CB Compact Scale
500g-3000g /
0.1g-1g
• Weighing
Compact, portable scale for a general
weighing
TBB - Triple Beam
Balances
2610g / 0.1g
• Weighing • Below balance
weighing • Density of solids
Low cost mechanical balances for high
precision, reliable weighing in laboratories,
schools and industry
Other Balances available so please contact us for more information.
features:
• Over 40 years’ experience in the manufacture of high quality
balances, weighing scales and mass measuring equipment
• Full versatility: systems supplied for weighing applications
requiring the greatest precision and sensitivity, as well as full
portability and space-saving design
• Full technical support and up to 3 years return-to-base
warranty
Typical Applications
Inert Gas Glove Boxes – handling and processing of air –
and moisture-sensitive materials in carefully maintained
atmosphere. Chemical Synthesis. Isolation Glove Box –
self-contained area for opening suspect packages.
VISIT www.cleaverscientific.co.uk
OR CALL +44 (0) 1788 565300
FOR MORE INFORMATION
Note: New Range coming soon.
Contact Cleaver Scientific for more
information.
• ISO 9001:2008 approved and certified
Typical Applications
Laboratories, research & education
Moisture analysis
Agriculture & food industries
visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
cleaver scientific Terms and Conditions
NOTES
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visit www.cleaverscientific.co.uk OR CALL +44 (0) 1788 565300 for more information
1.
1.1
Business customers and consumers
Some of these terms apply to consumers only; some apply to business customers only.
Those terms are marked as such.
1.2
All other terms apply to all customers.
1.3
You are classified as a business customer if you indicate to us that the goods supplied by
us will be used in the course of your business or if you use the goods in the course of your
business.
1.4
If you are not a business customer, you are a consumer. Nothing in these terms affects your
statutory rights as a consumer.
2.Price
2.1 Consumers: The quoted price includes VAT (unless otherwise stated).
2.2
Business customers: The price quoted excludes VAT (unless otherwise stated). VAT will
be charged at the rate applying at the time of delivery.
2.3
Our quotations lapse after twelve weeks (unless otherwise agreed).
2.4
Business customers: The price quoted excludes delivery (unless otherwise stated).
2.5
Business customers: Unless otherwise stated, the price quoted to business customers
is an illustrative estimate only and the price charged will be our price current at the time of
delivery.
2.6
Business customers: Rates of tax and duties on the goods will be those applying at the
time of delivery.
2.7
Business customers: At any time before delivery we may adjust the price to reflect any
increase in our costs of supplying the goods.
3.Delivery
3.1
All delivery times quoted are estimates only.
3.2
If we fail to deliver within a reasonable time, you may (by informing us in writing) cancel the
contract, however: 3.2.1
you may not cancel if we receive your notice after the goods have been dispatched; and
3.2.2
if you cancel the contract, you can have no further claim against us under that contract.
3.3
If you accept delivery of the goods after the estimated delivery time, it will be on the basis that
you have no claim against us for delay (including indirect or consequential loss, or increase
in the price of the goods).
3.4
We may deliver the goods in instalments. Each instalment is treated as a separate contract.
4
Delivery and safety
4.1
We may decline to deliver if:
4.1.1
we believe that it would be unsafe, unlawful or unreasonably difficult to do so; or
4.1.2
the premises (or the access to them) are unsuitable for our vehicle.
5
Payment terms
5.1
You are to pay us cash (or otherwise in cleared funds such as by credit card) on delivery
unless you have an approved credit account.
5.2
Business customers: If you have an approved UK business credit account, payment is due
30 days from the end of the month of the invoice , unless otherwise agreed in writing.
5.3
If you fail to pay us in full on the due date:
5.3.1
we may suspend or cancel future deliveries;
5.3.2
we may cancel any discount offered to you;
5.3.3
you must pay us interest at the rate equivalent to that set for the purposes of s6 of the Late
Payment of Commercial Debts (Interest) Act 1998:
a.
calculated (on a daily basis) from the date of our invoice until payment;
b.
compounded on the first day of each calendar month; and
c.
before and after any judgement (unless the court orders otherwise).
5.4
If you have an approved business credit account we may withdraw it or reduce your credit
limit or bring forward your due date for payment.
5.5
We take any of those actions in 5.4 at any time and without notice.
5.6
You do not have the right to set off any money you may claim from us against anything you
may owe us.
5.7
While you owe money to us, we have a right to keep any property we may hold of yours until
you have paid us in full (a lien).
5.8
You are to indemnify us in full and hold us harmless from all expenses and liabilities we may
incur (directly or indirectly and including legal costs on a full indemnity basis) following any
breach by you of any of your obligations under these terms.
6Title
6.1Consumers: s18 Sale of Goods Act 1979 applies.
6.2
Business customers: Until you pay all debts you may owe us:
6.2.1
all goods supplied by us remain our property;
6.2.2
you must store them so that they are clearly identifiable as our property;
6.2.3
you must insure them (against the risks for which a prudent owner would insure them) and
hold the policy on trust for us;
6.2.4
you may use those goods and sell them in the ordinary course of your business, but not if:
a.
we revoke that right (by informing you in writing); or
b.
you become insolvent.
6.3 Business customers: You must inform us (in writing) immediately if you become insolvent.
6.4
Business customers: If your right to use and sell the goods ends you must allow us to
remove the goods.
6.5
Business customers: We have your permission to enter any premises where the goods
may be stored:
6.5.1
at any time, to inspect them; and
6.5.2
after your right to use and sell them has ended, to remove them, using reasonable force if
necessary.
6.6
Despite our retention of title to the goods, we have the right to take legal proceedings to
recover the price of goods supplied should you not pay us by the due date.
6.7
You are not our agent. You have no authority to make any contract on our behalf or in our
name.
7Risk
7.1
The goods are at your risk from the time of delivery.
7.2
Delivery takes place either:
7.2.1
at our premises (if you are collecting them or arranging carriage); or
7.2.2
at your premises (if we are arranging carriage).
7.3
You must inspect the goods on delivery. If any goods are damaged (or not delivered), you
must write to tell us within seven working days of delivery (or the expected delivery time). You must give us (and any carrier) a fair chance to inspect the damaged goods.
8Warranties
8.1
We warrant that the goods:
8.1.1
comply with their description on our delivery note; and
8.1.2
are free from material defect at the time of delivery (as long as you comply with clause 7.3).
8.2
Business customers: We give no other warranty (and exclude any warranty, term or
condition that would otherwise be implied) as to the quality of the goods or their fitness for
any purpose.
8.3Consumers: Your statutory rights are unaffected.
8.4
If you believe that we have delivered goods which are defective in material or workmanship,
you must:
8.4.1
inform us (in writing), with full details, as soon as possible; and
8.4.2
allow us to investigate (we may need access to your premises and product samples). 8.4.3
If the goods are found to be defective in material or workmanship (following our
investigations), and you have complied with those conditions (in clause 8.4) in full, we will (at
our option) replace the goods or refund the price.
8.5
We are not liable for any other loss or damage (including indirect or consequential loss,
financial loss, loss of profits or loss of use) arising from the contract or the supply of goods or
their use, even if we are negligent.
Our total liability to you (from one single cause) for damage to property is limited to five
million pounds.
8.7
For all other liabilities not referred to elsewhere in these terms our liability is limited in
damages to the price of the goods.
8.8
Nothing in these terms restricts or limits our liability for death or personal injury resulting from
negligence.
9
Specifications and designs
9.1
If we prepare the goods in accordance with your specifications or instructions:
9.1.1
you must ensure that the specifications or instructions are accurate; 9.1.2
you must ensure that goods prepared in accordance with those specifications or instructions
will be fit for the purpose for which you intend to use them; and
9.1.3
you warrant that the specifications or designs will not result in the infringement of any rights
belonging to a third party and that you will indemnify us in respect of all loss, damage, costs
or expenses (including legal fees) which we may incur in connection with any such claim or
threatened claim by a third party.
9.2
By supplying goods to you we do not waive any intellectual property rights (including any
design rights) that we may have in respect of them.
9.3
Business customers only: we also reserve the right to make without notice any minor
modifications in our specifications designs or materials as we think necessary or desirable.
9.4
We reserve the right to make any changes in the specifications and designs of our goods
which are necessary to ensure they conform with any applicable safety or other statutory
requirements.
10
Return of goods
10.1
We will accept the return of goods from you only:
10.1.1
by prior arrangement (confirmed in writing);
10.1.2
on payment of an agreed handling charge (unless the goods were defective when delivered);
and
10.1.3
where the goods are as fit for sale on their return as they were on delivery.
11
Export terms
11.1
Where the goods are supplied by us to you by way of export from the United Kingdom then
the ‘Incoterms’ of the International Chamber of Commerce which are in force at the time of
the date when the contract is made shall apply.
11.2
If there is any conflict between the Incoterms and the terms in these terms of trading then
these terms shall prevail.
11.3
Clause 11 of these terms shall apply to exports (except where otherwise agreed in writing
between us) notwithstanding any other provisions of these terms.
11.4
You are responsible for complying with any legislation or regulations governing the
importation of the goods into the country of destination and for the payment of any duties
due.
11.5
The goods shall be delivered as agreed between us but we shall be under no obligation to
give a notice under section 32(3) of the Sale of Goods Act 1979.
11.6
You have the right at your expense and by arrangement with us to inspect the goods at our
premises or at the point of shipment before shipment except where otherwise agreed. If
you do not exercise that right then the fact the goods passed our quality inspection will be
conclusive proof that they were of the warranted quality. We are not liable for any defect
in the goods which would be apparent on your inspection unless a claim is made before
shipment. We are not liable for any damage during transit.
11.7
Payment of all amounts due to us shall be made as stipulated by us unless otherwise agreed
in writing.
11.8
We shall have no liability for death or personal injury arising from the use of the goods where
the goods are to be delivered in the territory of another State (within the meaning of s.26 (3)
(b) Unfair Contract Terms Act 1977).
12Cancellation
12.1
Suppose the order is cancelled (for any reason). You are then to pay us for all stock (finished
or unfinished) we may then hold (or to which we are committed) for the order.
12.2
We may suspend or cancel the order, by written notice if:
12.2.1
you fail to pay us any money when due (under the order or otherwise);
12.2.2
you become insolvent;
12.2.3
you fail to honour your obligations under these terms.
12.3
You may not cancel the order unless we agree in writing (and clauses 3.2.2 and 10.1 then
apply).
13
Waiver and variations
13.1
Any waiver or variation of these terms is binding in honour only unless:
13.1.1
made (or recorded) in writing;
13.1.2
signed on behalf of each party; and
13.1.3
expressly stating an intention to vary these terms.
13.2
All orders that you place with us will be on these terms (or any that we may issue to replace
them). By placing an order with us, you are expressly waiving any printed terms you may
have to the extent that they are inconsistent with our terms.
14
Force majeure - business customers only
14.1
Suppose we are unable to perform our obligations to you (or able to perform them only at
unreasonable cost) because of circumstances beyond our control. We may then cancel or
suspend any of our obligations to you, without liability,
14.2
Examples of those circumstances include act of God, accident, explosion, fire, transport
delays, strikes and other industrial disputes and difficulty in obtaining supplies.
15General
15.1
English law is applicable to any Contract made under these terms. The English and Welsh
courts have non-exclusive jurisdiction.
15.2
If you are more than one person, each of you has joint and several obligations under these
terms
15.3
If any of these terms are unenforceable as drafted:
15.3.1
it will not affect the enforceability of any other of these terms; and
15.3.2
if it would be enforceable if amended, it will be treated as so amended.
15.4
We may treat you as insolvent if:
15.4.1
you are unable to pay your debts as they fall due; or
15.4.2
you (or any item of your property) becomes the subject of:
a.
any formal insolvency procedure (examples of which include receivership, liquidation,
administration, voluntary arrangements {including a moratorium} or bankruptcy);
b.
any application or proposal for any formal insolvency procedure; or
c.
any application, procedure or proposal overseas with similar effect or purpose.
15.5
Business customers: all brochures, catalogues and other promotional materials are to be
treated as illustrative only. Their contents form no part of any contract between us and you
should not rely on them in entering into any contract with us.
15.6
Business customers only: any notice by either of us which is to be served under these
terms may be served by leaving it at or by delivering it to (by first class post or by fax) the
other’s registered office or principal place of business. All such notices must be signed.
15.7
No contract will create any right enforceable (by virtue of the Contracts [Rights of Third
Parties] Act 1999) by any person not identified as the buyer or seller.
15.8
The only statements upon which you may rely in making the contract with us, are those
made in writing by someone who is (or whom you reasonably believe to be) our authorised
representative and either:
15.8.1
contained in our estimate (or any covering letter) and not withdrawn before the contract is
made; or
15.8.2
which expressly state that you may rely on them when entering into the contract.
15.9
Nothing in clause 15.8 is to affect or limit our liability for fraudulent misrepresentation.
8.6
Cleaver Scientific Ltd
Unit 4 Triton Park
Brownsover Road
Swift Valley, Rugby,
Warwickshire CV21 1SG
United Kingdom
t: +44 (0)1788 565300
t: +44 (0)1788 552822
[email protected]
www.cleaverscientific.com
Distributor
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