EDP1 Single Channel User Manual 2004

EDP1 Single Channel User Manual 2004
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EDP1
™
10 pipettes for
volume ranges
from
0.5 µL to 20 mL
200 µL EDP1 shown
Page 1
Electronic Pipette
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Contents
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6
Section 7
Section 8
Section 9
Section 10
Section 11
Section 12
Appendix A
Appendix B
Description ...........................................................................1
Unpacking and Set-up ...........................................................1
Basic Operation ....................................................................4
Optional Settings .................................................................12
Setting Speed......................................................................13
Advanced Operation ............................................................14
Proper Pipetting Technique ...................................................18
Removing the Tip Ejector......................................................19
Changing Liquid Ends .........................................................19
Care and Maintenance.........................................................22
Troubleshooting ..................................................................24
Specifications .....................................................................25
Recharging the Battery Pack.................................................26
Ordering Information............................................................27
FIGURES AND TABLES
Figure 1-1
Figure 2-1
Figure 2-2
Figure 3-1
Figure 3-2
Figure 8-1
Figure 9-1
Figure 9-2
Figure 9-3
Figure 9-4
Figure 9-5
Figure 10-1
Figure 10-2
Figure A-1
Figure A-2
Figure B-1
EDP1 Controls and Indicators.................................................1
Display after Initialization Sequence ........................................2
Installing the Battery Pack ......................................................3
EDP1 Keyboard ....................................................................4
Resuming Operation after Auto Shut-Off ...................................5
Removing the Tip Ejector......................................................19
Removing the Liquid End .....................................................20
Removing the Encoder Plug .................................................20
Replacing the Liquid End .....................................................21
Replacing the Encoder Plug..................................................21
Display after Initialization Sequence ......................................21
Detail View of Liquid End (200 µL shown).............................22
Piston Seal Assembly (200 µL shown)..................................23
Connecting Wall Power Supply .............................................26
Rapid Charge Stand Accessory .............................................26
Line drawings of EDP1 Models .............................................29
Table 3.1
Table 5.1
Table 6.1
Table 11.1
Table 12.1
Basic Operation ....................................................................4
Programmable Speeds ........................................................13
Advanced Functions ............................................................14
Troubleshooting Table..........................................................25
Specifications .....................................................................25
This device (EDP1) is intended for use only as an electronic pipette for pipetting liquids as described in this manual. It is not
intended for any other use.
Note that pipette specifications and other information in this manual may be changed without prior notice.
“EDP1” and “EDP” are trademarks of Rainin Instrument, LLC. EDP1 Pipettes are manufactured under U.S. Patent No.
4,671,123; 4,779,467 and 4,905,526, and under the following national patents: Taiwan, NI-23042; Australia,
AU 589891; France, FR 2559904; Europe: CH, DE, GB, IT, NL, EP 0152120 and EP 0428500; Canada,
CA 1293709; Japan, JP 1807271; Germany, DE 3588071 and DE 3586289.
©2004, Rainin Instrument, LLC. All rights reserved.
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Description
1
EDP1 Electronic Pipettes use a motorized linear actuator to move the piston. The linear
actuator, controlled by a miniature onboard computer, moves the piston the correct distance,
at the correct speed, every time. Piston strokes are consistent and reproducible.
Each complete EDP1 pipette includes a control
module, battery pack, a wall power supply, and a
liquid end for the volume range ordered. The control
module is the same for all volume ranges; an
encoder plug informs the control module which liquid
end is attached. For volumes up to 2000 µL,
interchangeable liquid ends with encoders can be
purchased separately.
A stainless steel piston is moved by an electronic
linear actuator. The piston displaces a column of air
which picks up or dispenses liquid through a
disposable polypropylene tip. This tip is the only part
of the instrument that touches the liquid. A
mechanical tip ejector allows tips on all volume
pipettes to be ejcted easily without hand contact,
avoiding sample carryover and contamination.
You enter mode, volume, and speed settings into the
EDP1 via a keyboard. Operational status and settings
are shown on an easy-to-read display. Audible tones
signal completion of piston strokes and error
conditions. Pickup and dispense are performed by
pressing the trigger.
Power can be supplied to the EDP1 pipette in either of
two ways: with a cord attached to a wall power
supply, or with a rechargeable Battery Pack for
maximum convenience and freedom of movement.
With a fully-charged Battery Pack installed, the EDP1
will operate for more than 1000 full-stroke cycles
before recharging is needed.
LCD (Liquid
Crystal Display)
Encoder Plug
Keyboard
Tip Ejector
Button
Trigger
Control
Module
Tip Ejector
Arm
Shaft
Coupling
Interchangeable
Liquid End
Disposable
Tip
FIGURE 1-1
EDP1 CONTROLS AND INDICATORS
Unpacking and Set-up
2
In addition to this manual, the EDP1 package should contain:
1. EDP1 Electronic Pipette
3. Wall Power Supply
2. Battery Pack
4. Performance Check Report/Warranty card
Before using your EDP1, inspect each component for damage sustained in transit. If you
find damage, file a claim immediately with the shipping carrier. Save the shipping container
until any damage claim is resolved and the instrument is checked and operates properly.
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WARRANTY
Complete and return the Warranty Registration Card. EDP1 Electronic Pipettes carry a one year
warranty against defects in materials and workmanship. Physical and chemical abuse are not
covered. For information regarding the warranty or repair of any instrument, please call RAININ at 800543-4030, e-mail: [email protected]
Outside the U.S.A., local warranty conditions may apply. Contact your RAININ distributor: see
www.rainin-global.com or e-mail [email protected]
NOTE: If the equipment is used in a manner not specified, the protection provided by the
equipment may be impaired.
Warranty returns are only accepted with prior authorization.
SET-UP
Connect the wall power supply to a power outlet matching the line voltage printed on the label. Then
plug the cord from the power supply into the socket on the back of the control module.
The EDP1 pipette will execute its initialization routine. After about 5 seconds the instrument will be in
PIPETTE mode and the display should be as shown in Figure 2-1. If not, refer to the Troubleshooting
Guide in Section 11 of this manual.
PICKUP
KB
FIGURE 2-1
1000µl
1000µl
DISPLAY AFTER INITIALIZATION SEQUENCE (E1-1000 SHOWN)
NOTE: EDP1 can be used without a power cord when the Battery Pack is installed.
However, before installing the Battery Pack, it is a good idea to test operation with the
wall power supply, to see that the pipette works correctly.
Your EDP1 pipette is now ready for use, but the Battery Pack is not yet installed. Install the Battery
Pack into the control module as described in the following pages.
WARNING:
This equipment generates and uses radio frequency energy and if not installed and used properly, i.e., in strict
accordance with the instruction manual, may cause harmful interference to radio communications. It has been tested
and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC rules,
which are designed to provide reasonable protection against such interference when operated in a commercial
environment.
Operation of this equipment in a residential area is likely to cause interference, in which case the user at his own
expense will be required to take whatever measures may be required to correct the interference.
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INSTALLING THE BATTERY PACK
The Battery Pack compartment is at the rear of the control module. Slide the battery
compartment cover left to remove it. Carefully pull the male connector clear, shown below.
Align the male and female connectors, noting the pin arrangement: one pin, space, two
pins. Fasten the connectors together, but do not force them. Insert the Battery Pack into the
control module as indicated on the label. Replace the compartment cover.
If the pipette does not initialize when the Battery pack is installed, but worked normally
when connected to the wall power supply, the Battery Pack may have discharged in transit.
See Appendix A – Charging the Battery Pack.
A
FEMALE
CONNECTOR
BATTERY PACK
CONTROL MODULE
MALE
CONNECTOR
B
Connect the male connector to the female connector on the new battery pack, and rotate the
battery pack into the control module as shown by the arrow.
C
Push the battery pack into the control module as far as possible, as shown at left. Push the
wires to the right of the battery as shown at right. Replace the battery door and slide it
closed.
FIGURE 2-2
INSTALLING THE BATTERY PACK
If you have any questions regarding this procedure, call Technical Support: 800-543-4030.
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Basic Operation
3
EDP1 can operate in five modes:
MODE
KEY
DESCRIPTION
P: PIPETTE
F 1
A single volume is picked up and dispensed.
M: MULTIPLE
DISPENSE
F 2
A single volume is picked up and multiple
volumes dispensed stepwise.
T: TITRATE
F 3
A single volume is picked up and partially
dispensed under trigger control.
D: DILUTE
F 4
Diluent, an air gap, and sample are picked up
separately and dispensed together.
MEAS:
MEASURE
F 5
A sample is picked up under trigger control
and its volume is displayed.
Table 3.1 – EDP1 Basic Operation
Modes are selected in EDP1 by pressing the F key and a
number key as shown in the table above. The keyboard
shows the name or initial of the mode. See Figure 3-2:
The instrument powers up in PIPETTE mode.
You can change modes when:
FIGURE 3-1
EDP1 KEYBOARD
1. The piston is in the HOME position and KB is displayed. (If necessary, press the
FUNCTION key F and then 0 to clear the current operation and move the piston to HOME.
KB should then appear on the display.)
2. The keyboard is not locked. (Press F 9 to unlock.)
To change modes press F then the appropriate number key. For example, to open TITRATE
mode press F 3. For MULTIPLE DISPENSE mode, press F 2.
The EDP1 pipette has been designed to be easy to use whether you are right- or lefthanded. The balance, weight, comfortable “feel”, and light touch of EDP1 make it ideal for
continuous use.
Hold EDP1 loosely in either hand – you do not need to grip the pipette tightly during
operation. Operate the trigger with your index finger and the tip ejector button with your
thumb. Because there is no need for thumb pressure during pick up, dispense, and
blowout, operator fatigue and the risk of repetitive strain injury are greatly reduced compared
to manual pipettes.
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AUTO SHUT-OFF
The EDP1 pipette shuts itself off automatically if none of the keys or the trigger are pressed
for three minutes. If the display is blank and the trigger does not work while power is
supplied, EDP1 is shut off. Press any of the top row keys or E to turn on the instrument.
V SEQ
F
L
7
8
9
D
MEAS
MIX
4
5
6
C
P
M
T
0
1
2
3
FIGURE 3-2
S
.
Press any key in this row
or E
When EDP1 is shut off
E
RESUMING OPERATION AFTER AUTO SHUT-OFF
EDP1 remembers current operational settings when “shut-off”, and will resume operation at
the same point and with the same settings as when last used, as long as power to the
instrument has not been interrupted.
The AUTO SHUT OFF feature is provided to extend the life of the Battery Pack.
BASIC OPERATION
The examples used in this manual show the display for E-1000 (EDP1 with 1000 µL
nominal volume). Your instrument will operate in the same way, but volumes shown will be
different if your pipette has a different nominal volume.
Attach a disposable tip by pressing the liquid end shaft firmly into the tip. Use water as your
sample until you are familiar with the EDP1 pipette.
QUICK REFERENCE:
PIPETTE MODE
Ready
Pick up
Dispense
Blowout
Ready
PISTON
POSITION
UP
HOME
DOWN
TRIGGER> > >
TRIGGER> > >
PAUSE > > >
PAUSE > > >
Holding in the
trigger delays
this step
BEEP
BEEP
5
BEEP
BEEP
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PIPETTE MODE
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F 1
When power is supplied, EDP1 executes a five-second initialization sequence and opens in
PIPETTE mode. The display will show the nominal volume.
SETTINGS
Press number keys then the E (Enter) key to change the volume. The display will flash on
and off. If you make a mistake, set a new volume then press E.
OPERATION
PICKUP
KB
DISPENSE
475µl
475µl
PICKUP VOLUME
DISPENSE VOLUME
1. Press and release the trigger* to pick up the displayed volume. You will hear a “beep” at
the end of the piston stroke. See diagram on previous page.
2. Press and release the trigger again to dispense the liquid.
The piston moves to HOME, then rapidly executes a blowout stroke. You will hear a beep at
the end of the blowout. After a one-second delay the piston returns to HOME ready for the
next pickup. This is signalled by two beeps**.
The one-second delay is to give you time to remove the tip from the dispense vessel before
the piston returns to HOME position. You can extend the delay by holding in the trigger as
the sample is dispensed.
When EDP1 is in any other mode, open PIPETTE mode as follows:
a. Press F 0. This action will clear the current operation and move the piston to HOME
position. KB will show on the display, indicating the piston is HOME. (Check that the
keyboard is not locked. If locked, press F 9 to unlock it.)
b. Press F 1 to open PIPETTE mode.
The display will show the nominal volume unless you have previously set a volume in
PIPETTE mode. (In all modes, any previously entered volume is remembered by EDP1 as
long as power is supplied continuously.)
* Holding in the trigger postpones the next step in all modes except TITRATE, MEASURE, and MIX.
** Each piston stroke is signalled by a short beep when the tone generator is turned on.
End-of-stroke beeps will not be included in the following descriptions.
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QUICK REFERENCE:
Page 9
MULTIPLE DISPENSE MODE
Ready
Dispense
Multiple
Pick up
Blowout
Ready
PISTON
POSITION
UP
HOME
DOWN
REPEAT
TRIGGER> > >
TRIGGER> > >
BEEP
CLEAR > > >
BEEP (AFTER
EACH ALIQUOT)
PAUSE > > >
BEEP
BEEP
BEEP
BEEP
MULTIPLE DISPENSE MODE F 2
Make sure the piston is at the HOME position (KB shows on display) and the keyboard is
not locked. Open MULTIPLE DISPENSE mode by pressing F 2 .
PICKUP
MULTI
MULTIPLE DISPENSE NOMINAL VOLUME
1000µl
1000µl
KB
SETTINGS
1. The nominal pickup volume (or previously stored value) is displayed. Press E to check
or change the dispense volume.
2. Press number keys to set or change the dispense volume. While pressing keys, the entry
flashes until E is pressed. See DISPENSE VOLUME, below.
3. The instrument calculates and flashes the largest number of whole aliquots which will
divide into the nominal volume. You can accept this aliquot number by pressing E , or you
can change to a smaller number by pressing number keys, then E.
MULTI
KB
DISPENSE
MULTI
235µl
235µl
KB
DISPENSE VOLUME
44
NUMBER OF ALIQUOTS (FLASHING)
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4. The instrument calculates and displays the volume needed to pick up all aliquots.
PICKUP
940µl
KB
TOTAL PICKUP VOLUME
MULTI
OPERATION
1. Press and release the trigger to pick up the displayed volume.
2. Press and release the trigger again to dispense the first aliquot. Continue pressing and
releasing the trigger until all aliquots are dispensed. The word CLEAR will appear on the
display.
3. Press 0 to clear the instrument, discarding the residual volume left in the tip.
After the dispense volume is entered, EDP1 calculates the pickup volume:
Pickup Volume = (Number of Aliquots x Dispense Volume) + Residual Volume
When the calculated volume is picked up, the piston slightly overshoots and then
returns to its proper pickup position, to ensure accuracy of the first aliquot
dispensed. To ensure that the last aliquot will be accurate, the fixed residual
volume remains in the tip after all aliquots have been dispensed and must be
discarded by pressing 0 before making the next pickup stroke.
QUICK REFERENCE: TITRATE MODE
Ready
Pick up
Titrate
Discard remainder
and Blowout
Ready
PISTON
POSITION
UP
HOME
DOWN
TAP or HOLD
TRIGGER> > >
TRIGGER> > >
F 0 to
CLEAR > > >
BEEP
PAUSE > > >
BEEP
READ VOLUME
DISPENSED
8
BEEP
BEEP
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TITRATE MODE F 3
Make sure the piston is at the HOME position (KB shows on display) and the keyboard is
not locked.
TITRATE
PICKUP
Open TITRATE mode by pressing F 3 .
KB
1000µl
TITRATE NOMINAL VOLUME
SETTINGS
1. The display shows the nominal pickup volume.
To change the pickup volume, enter a new initial
pickup volume with the number keys and press E .
TITRATE
PICKUP
KB
560µl
INITIAL PICKUP VOLUME SET
2. To set an initial dispense volume, press number
keys then press E . (To set an initial dispense
volume without changing the initial pickup volume,
press E first. Enter the volume with number keys.
Then press E again.)
TITRATE
KB
DISPENSE
150µl
INITIAL DISPENSE VOLUME SET
OPERATION
1. Press and release the trigger to pick up the initial volume.
2. To begin titration, press and hold the trigger to dispense liquid first slowly, then at an
increasing rate. The longer the trigger is held, the faster the liquid is dispensed. Release
and hold in the trigger again to restart dispensing at the slowest rate. To dispense one
increment at a time, tap the trigger.
3. When the desired endpoint is reached, read and record the dispensed volume from the
display. Then clear the instrument by pressing F 0 . This will discard the remainder in
the tip and return EDP1 to its starting position.
If the entire volume is titrated, press the 0 key when the beep sounds and the display
reads “CLEAR”. This action will execute the blowout stroke and empty the residual volume
from the tip.
In TITRATE mode, a small residual volume is left in the tip after the entire volume has been
titrated. The residual volume ensures that the volume titrated is accurate.
The residual volume is not part of the titrated volume and must be discarded by
pressing F 0 .
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QUICK REFERENCE:
DILUTE MODE
Pick up
Diluent V1
Ready
Page 12
Pick up
Air
Pick up
Sample V2
Blowout
Dispense
Ready
PISTON
POSITION
UP
HOME
DOWN
TRIGGER> > >
TRIGGER> > >
TRIGGER> > >
TRIGGER> > >
PAUSE > > >
PAUSE > > >
Holding
trigger
delays this
step
DILUENT
AIR GAP
SAMPLE
BEEP
BEEP
BEEP
BEEP
BEEP
BEEP
BEEP
BEEP
DILUTE MODE F 4
Make sure the piston is at the HOME position (KB shows on display) and the keyboard is
not locked. Press F 4 to open DILUTE mode. The display will show the default diluent
volume, which is 90% of nominal volume. (The default sample volume is 10% of nominal
volume, for 10:1 dilution.)
SETTINGS
1. Set the diluent volume (V1) by pressing number keys then E .
DILUTE
DILUENT VOLUME
KB
500µl
500µl
V1
2. Set the sample volume (V2) in the same way.
DILUTE
SAMPLE VOLUME
KB
100µl
100µl
V2
OPERATION
1. Press and release the trigger to pick up the diluent.
DILUTE
PICKUP
PICK UP DILUENT VOLUME
10
KB
500µl
500µl
V1
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2. Press and release the trigger to pick up an
air gap*.
Air
DILUTE
PICKUP
3. Press and release the trigger a third time to pick
up the sample.
100µl
V2
DILUTE
4. A three-part tone sounds, indicating that diluent
and sample are separated by the air gap, ready to
be dispensed. Press and release the trigger to
dispense diluent and sample.
DISPENSE
600µl
DISPENSE DILUENT AND SAMPLE TOGETHER
5. The display shows the diluent volume, ready for the next cycle.
* When using DILUTE mode with the 5 mL, 10mL, and 20 mL EDP1 models, the air gap will
probably rise through the diluent and allow diluent and sample to mix in the sample reservoir. To
avoid this, you can use VOLUME SEQUENCING in DILUTE mode. Then set a smaller air gap, or no
gap, as desired. See Section 6 — Advanced Operation: VOLUME SEQUENCING.
QUICK REFERENCE:
MEASURE MODE
Ready
Discard
remainder
Pick up
Blowout
Ready
PISTON
POSITION
UP
HOME
DOWN
TAP or HOLD
TRIGGER> > >
F 0 to
CLEAR > > >
PAUSE > > >
PAUSE > > >
Holding
trigger
delays this
step
READ VOLUME
MEASURED
BEEP
BEEP
BEEP
NOTES :
1. An initial pickup volume can be set.
2. Piston direction can be changed by pressing 0 , even while the trigger is pressed.
3. At full stroke (or at zero) the trigger must be released.
3. Then either press F 0 to clear the pipette, or press the trigger again to reverse piston direction.
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MEASURE MODE F 5
Make sure the piston is at the HOME position (KB shows on display) and the keyboard is
not locked. Press F 5 to open MEASURE mode.
SETTINGS
The display reads “0µl” (or any previously entered initial pickup volume). You can enter an
initial pickup volume if you wish by pressing number keys then E .
OPERATION
1. Press and hold the trigger to pick up. Pickup speed increases until it reaches the
maximum. Release the trigger and press it again to restart at the slowest rate. To pick up
one increment at a time, tap the trigger. (Any initial pickup volume is picked up at the first
trigger press. Hold the trigger in to continue measurement.)
When the desired volume of liquid has been picked up, ensure the lower level of the liquid
is flush with the tip orifice (no air has been included) and read the volume on the display.
The piston direction can be reversed at any time by pressing 0 . (This is useful if an air
bubble is picked up; pressing 0 reverses the flow, expelling the bubble. Pressing 0
again reverses flow and continues measurement.) The display always shows the current
volume in the tip as well as the piston direction, either PICKUP or DISPENSE.
2. At full- or zero-scale, the display reads “CLEAR” and the trigger must be released.
Press the trigger again to reverse direction automatically. (The pipette may be cleared and
the tip contents dispensed by pressing F 0 ; this is useful if the volume of liquid to be
picked up exceeds the full-scale volume of the instrument.)
Optional Settings
4
TONE GENERATOR CONTROL F 8
A tone generator in the EDP1 signals operational status or error conditions as follows:
Status
Tone sound
Tone description
Initialization
Key press
End of pickup stroke
End of dispense stroke
Piston at HOME
End of pickup in DILUTE mode
Error condition/Low battery*
BEEP-BEEP
BIP
BEEP
BEEP
BEEP-BEEP
BEEP-BEEP-BEEP
DEE-DAH-DEE-DAH
Two short beeps
One very short beep
One short beep
One short beep (lower tone)
Two short beeps
Two short, one long beep
High warble
* Works even when tone generator is OFF
Tones are automatically switched on when the instrument is powered up. To switch off the
tones, press F 8 . Pressing F 8 again turns the tone generator back on.
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KEYBOARD LOCK CONTROL F 9
The EDP1 keyboard can be locked if desired. This feature is for protection. Locking the
keyboard prevents unwanted volume or mode changes when the EDP1 is used for the same
task over an extended period.
To lock the keyboard, press F 9 when the keyboard is active (when KB is displayed).
Press F 9 again to unlock the keyboard. When the keyboard is locked, all keystrokes are
ignored excep F 0 (clear), F 8 (tone generator control), and F 9 (keyboard
lock/unlock). The trigger operates whether the keyboard is locked or unlocked.
Setting Speed
5
PROGRAMMABLE SPEED
F •
You can program the pickup and dispense speed of the EDP1 pipette to accommodate
almost any liquid, including moderately dense or viscous liquids.
The default speed setting for EDP1 pipettes is 8*. This fast setting is optimized for liquids
with similar physical properties to water. However, you may wish to set a higher speed for
even faster operation or a slower speed for more dense or viscous liquids.
To reset the speed, press F • (decimal point) then press a number between 0 and 9, then
press E . The display reverts to show volume after speed has been set. The table below
shows cycle component times for available speed settings.
Table 5.1 – EDP1 Programmable Speeds
Speed
Setting
Full-Scale
Pickup
Full-Scale
Dispense
Delay before
Blowout
Blowout
duration
Dwell Time
(constant)
9
1.3 sec.
1.3 sec.
0.0 sec.
0.13 sec.
1.0 sec.
8
1.3 sec.
1.3 sec.
0.5 sec.
0.13 sec.
1.0 sec.
7
1.3 sec.
1.3 sec.
1.0 sec.
0.13 sec.
1.0 sec.
6
2.2 sec.
2.2 sec.
0.5 sec.
0.22 sec.
1.0 sec.
5
2.2 sec.
2.2 sec.
1.0 sec.
0.22 sec.
1.0 sec.
4
2.2 sec.
2.2 sec.
1.5 sec.
0.22 sec.
1.0 sec.
3
2.2 sec.
2.2 sec.
2.0 sec.
0.22 sec.
1.0 sec.
2
4.4 sec.
4.4 sec.
0.5 sec.
0.44 sec.
1.0 sec.
1
4.4 sec.
4.4 sec.
1.0 sec.
0.44 sec.
1.0 sec.
0
4.4 sec.
4.4 sec.
2.0 sec.
0.44 sec.
1.0 sec.
This table does not apply to trigger-controlled actions in TITRATE and MEASURE modes.
* Default speed setting for EDP1 10 mL, 20 mL models is 5. Speed range is 0 – 6.
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Advanced Operation
6
Table 6.1 – EDP1 Advanced Functions
FUNCTION
KEY
DESCRIPTION
APPLICABLE MODE
MIX
F 6
Mixes by repeated up-anddown piston motion while
trigger is held in
P – PIPETTE
D – DILUTE
VOLUME
SEQUENCING
F 7
PIPETTE: up to 12 distinct
volumes can be picked up
and dispensed sequentially.
P – PIPETTE
MULTIPLE DISPENSE: up
to 12 distinct volumes can
be dispensed sequentially
from a single pickup volume.
M – MULTIPLE DISPENSE
DILUTE: up to 12 distinct
volumes can be picked up
sequentially and dispensed
together.
D – DILUTE
MIX
F 6
Available in PIPETTE and DILUTE modes only.
SETTINGS
1. Open the MIX function by pressing F 6 .
2. The display prompts you for a mix volume*.
Enter this volume and press E .
KB
200µl
V
MIX
MIX VOLUME ENTERED
OPERATION
IN PIPETTE MODE: 1. Press and release the trigger to aspirate the pickup volume. Press
the trigger again to dispense.
IN DILUTE MODE: 1. Press and release the trigger to pick up the diluent. Press and
release the trigger to pick up an air gap, and again to pick up the sample. When the threepart tone sounds, press the trigger once more to dispense diluent and sample together.
* The mix volume may be larger than the pickup or diluent/sample volumes.
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2. Hold the trigger (either during or after the dispense stroke) to start mixing. Mixing will
continue as long as the trigger is held in. The display alternates between PICKUP and
DISPENSE while mixing is in progress.
DISPENSE
PICKUP
200µl
V
200µl
MIX
V
MIX
MIXING IN PROGRESS; MIX VOLUME ALTERNATELY PICKED UP AND DISPENSED
3. Release the trigger. Mixing stops and a normal blowout stroke occurs. The display
shows the pickup or diluent volume, ready for another cycle.
4. To close the MIX function press F 6 .
VOLUME SEQUENCING F 7 in PIPETTE, MULTIPLE DISPENSE, and DILUTE modes.
VOLUME SQUENCING: PIPETTE MODE
In PIPETTE mode, up to twelve distinct volumes can be picked up and dispensed
sequentially with VOLUME SEQUENCING.
When using this function, you are not restricted to the fixed sequence: by pressing the
F 0 keys you can go back and repeat the previous volume, or by pressing E you can
skip over the next volume. The sequencing cycle can be started at any point. Open the
VOLUME SEQUENCING function by pressing F 7 .
SETTINGS
Display flashes “0µl” (or the volume last set). Set the first pickup / dispense volume (V1) by
pressing number keys followed by E . Continue setting sequential pickup/dispense
volumes. Press 0 E to end the sequence. If you enter all twelve volumes, you do not
have to press 0 to end the sequence. Sequential volumes are numbered as follows:
V1–9, then VA (10), Vb (11), & VC (12).
OPERATION
1. Press and release the trigger to pick up the current volume.
PICKUP
KB
SEQ
100µl
FIRST VOLUME PICKED UP
15
V1
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2. Press and release the trigger to dispense the current volume.
DISPENSE
100µl
SEQ
V1
1ST VOLUME DISPENSED
3. Press and release the trigger to pick up the next sequential volume.
PICKUP
KB
SEQ
150µl
V2
2ND VOLUME PICKED UP
4. Press and release the trigger to dispense the next sequential volume. Continue in this
way until all volumes are picked up and dispensed.
5. After the last volume in the sequence is dispensed, the first volume is displayed again,
ready for pickup.
6. To close the VOLUME SEQUENCING function, press F 7 .
VOLUME SEQUENCING: MULTIPLE DISPENSE MODE
In MULTIPLE DISPENSE mode with VOLUME SEQUENCING, up to 12 distinct volumes can be
dispensed sequentially from one pickup volume. Open the VOLUME SEQUENCING feature by
pressing F 7 .
SETTINGS
1. The display flashes “0µl” (or the volume last set). Enter the first volume (V1) with the
number keys and press E .
2. The display flashes “0µl” again, prompting you for the next volume. Enter the second
volume (V2) and continue in the same way for each entry, finishing each entry with
E and the last entry with 0 E . Sequential volumes are numbered as follows: V1–9, then
VA (10), Vb (11), & VC (12).
3. The EDP1 pipette calculates and displays the number of cycles, or the largest number of
times the sum of the volumes will divide into the nominal volume of the instrument. Accept
the number of cycles by pressing E , or set a smaller number and press E . (Setting a
larger number will sound an error alarm.)
OPERATION
1. The display will show the volume to be picked up; press and release the trigger to pick
up that volume.
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2. Press and release the trigger again to dispense the first sequential volume. Press and
release the trigger again to dispense the second sequential volume, and so on.
3. After all sequential volumes in the first cycle have been dispensed, press the trigger to
begin the second cycle. Continue until all cycles are complete.
4. The display will read “CLEAR” after the last dispense volume in the last cycle. Press 0
to clear the instrument.
5. To close the VOLUME SEQUENCING function, press F 7 .
VOLUME SEQUENCING: DILUTE MODE
In DILUTE mode with VOLUME SEQUENCING, up to twelve distinct volumes can be picked
up sequentially and dispensed together. Open VOLUME SEQUENCING by pressing F 7 .
SETTINGS
1. Display flashes “0µl” (or volume last set). Press number keys then E to set the first
sequential volume (V1).
2. Display flashes “0µl” (or volume last set). Press number keys followed by E to set the
next volume (V2). Sequential volumes are numbered as follows: V1–9, then VA(10),
Vb (11), & VC (12).
3. Continue in this way until all volumes are set. Press 0 E to end the sequence.
OPERATION
1. The display shows the first pickup volume. Press and release the trigger to pick up the
first volume (V1).
2. Press and release the trigger again to pick up the next volume (V2). Air gaps are not
automatically added when VOLUME SEQUENCING. If you wish, air gaps can be set as
volume entries.*
3. Continue in this way until all volumes are picked up.
4. A three-part tone sounds. Press the trigger to dispense the total volume all at once.
5. The display shows the first pickup volume again. Press and release the trigger to pick up
volume V1 and restart the cycle.
6. To close the VOLUME SEQUENCING function, press F 7 .
* This is a good way to avoid the potential problem when using DILUTE mode with the 5 mL,10mL, and
20 mL EDP1 models — where the air gap can rise through the diluent, allowing diluent and sample to
mix in the sample reservoir. You can set a smaller air gap which will stay in place between the diluent
and sample, or no gap at all. See Section 3 — Basic Operation, DILUTE mode.
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Proper Pipetting Technique
7
Use RAININ tips
EDP1 pipettes are designed for use with RAININ-manufactured disposable tips. Specified
performance of EDP1 pipettes is guaranteed only when RAININ disposable tips are used.
RAININ cannot accept responsibility for damage to EDP1 pipettes or for poor performance
resulting from the use of other than RAININ disposable tips.
RAININ molds its own disposable tips from premium-grade virgin polypropylene plastic in
an advanced clean-room environment. Molds are inspected and maintained frequently to
assure high-quality tip production. Samples from each lot of tips are inspected
microscopically to assure that every lot meets these high standards. To order tips for EDP1
pipettes, please refer to the RAININ Pipetting Solutions Catalog or the current RAININ
Product Update. Call 800-543-4030 for a free copy of these publications. You can also
view or download a PDF version online: http://www.rainin.com/lit_prodlit.asp
Pipetting techniques
EDP1 pipettes are designed to deliver accurate and precise measurements under the
following conditions:
1. The liquid pipetted is water or liquid with density, viscosity, and vapor pressure similar to
water. The temperature of the room, the EDP1, and the liquid, are all in the range 21.5°C ±
1°C. The EDP1 pipette is kept vertical while pipetting or within 20° of vertical.
2. The end of the disposable tip is immersed beneath the sample surface as follows:
2 to 3 mm – volumes up to 100 µL.
2 to 4 mm – volumes from 100 µL to 1000 µL.
3 to 6 mm – volumes from 1000 - 5000 µL.
6 to 10 mm – volumes over 5000 µL
3. There is no significant adherence of liquid to the surfaces of the tip. (Each tip should be
pre-rinsed twice before pipetting to assure the best reproducibility).
4. When dispensing, the tip is touched to the sidewall of the receiving vessel. At the end of
each dispense cycle, the tip is moved along the wall to remove any excess sample from the
outside of the tip. A consistent rhythm is maintained from sample to sample when pipetting.
Viscous liquids, such as serum or plasma, can be measured accurately with a slow speed.
See Table 5.1 – Programmable Speeds. You can further improve accuracy with viscous
solutions by using MULTIPLE DISPENSE mode with the number of aliquots set to 1 and
using a slow speed. This method simulates “reverse-mode” pipetting in manual pipettes. In
MULTIPLE DISPENSE mode, the volume picked up includes an extra residual volume and
the volume of the dispensed aliquot is accurate.
You can measure warm or cold liquids with good precision by using a consistent pipetting
rhythm. A consistent rhythm will help minimize any differences in heating or cooling effects
within the pipette. Use a new disposable tip each time for best accuracy and precision when
measuring samples with temperatures greatly different from ambient, and do not pre-rinse.
As with any air-displacement pipette, best results will be obtained if there is no delay
between picking up the sample and dispensing it.
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Removing the Tip Ejector Arm
8
REMOVING THE TIP EJECTOR
The tip ejector should be removed to change the liquid end on models up to 2000 µL (see
Section 9 below). It can also be removed if you wish to load and remove tips manually for
pipetting into deep narrow vessels, especially on the larger-volume models. Three types of
tip ejector are used and all are removed with minimum effort—do not use force.
Up to 2000 µL
5000 µL, 10 mL
20 mL
FIGURE 8-1 REMOVING THE TIP EJECTOR ARM
For models up to 2000 µL, press in the quick-release tabs on the ejector arm and pull the
ejector down. For 5000 µL & 10 mL models, twist the end of the ejector arm and pull
downward. For 20 mL models, pull the lower part of the tip ejector away from the upper
part (do not remove the upper part).
To replace the ejector arm (after changing the liquid end) on all models except 20 mL,
insert the shaft through the large opening, align the top with the tip ejector pushrod, and
push until the ejector arm snaps in place. For 20 mL models, place the large opening over
the shaft and align the rod in the lower part of the tip ejector with the hole in the upper part
and press firmly.
Changing Liquid Ends
9
The liquid ends for models up to 2000 µL are interchangeable. Liquid ends are not
interchangeable on 5000 µL, 10 mL and 20 mL models.
When changing a liquid end, you must also change the encoder plug on the control
module, to signal the liquid end volume to the software. Both the encoder plug and the shaft
are marked with the nominal volume. LTS shafts are used with encoder plugs marked LTS
and the nominal volume. Traditional shafts are used with encoder plugs marked with the
nominal volume only.
Before removing the liquid end, bring the piston to HOME by pressing F 0 . The letters KB
should appear on the display. If the keyboard is locked, unlock it (press F 9 ).
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A
Page 22
B
C
BALL-JOINT
IN SOCKET
BALL-JOINT
FIGURE 9-1
REMOVING THE LIQUID END
(NOTE THE BALL-JOINT CONNECTION IN B & C)
TO REMOVE THE LIQUID END
1. Referring to Figure 9-1A , loosen the shaft coupling by rotating it counter-clockwise.
2. When the shaft coupling is free of the threads, remove the shaft coupling. The motor
shaft is connected to the piston assembly by a ball-joint. This ball-joint must be
disconnected before proceeding, as follows:
a. pull the liquid end away from the control module enough to reveal the end of the
motor shaft – see Figure 9-1B. The spring and clear plastic spring holder can be left
in place: they will not interfere with this procedure.
b. angle the liquid end and ease the ball-joint out of the socket – see Figure 9-1C.
3. Remove the encoder plug from the control module (see
Figure 9-2) and store it with its matching liquid end. Liquid
measurements made with the EDP1 will be accurate and
precise ONLY when the correct liquid end/encoder plug
combination is used.
FIGURE 9-2 REMOVING
THE ENCODER PLUG
NOTE: Before storage, disassemble the liquid end (see Section 10) and
inspect for salts or residues. Clean the liquid end and store it with its
encoder plug and tip ejector arm in the two tubes provided with the
replacement liquid end.
Do not touch the underside of the plug; skin oils can contaminate it,
causing poor electrical contact and erratic performance. The plug can be
cleaned with isopropanol.
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REINSTALLING THE LIQUID END
CAUTION: Always attach the replacement liquid end BEFORE inserting the encoder plug.
1. The replacement liquid end is supplied in a clear plastic tube together with a second tube
holding the matching tip ejector and encoder plug. Make sure you have all three
components. The spring and spring guide extend from the liquid end as shown in Figure
9-3A. It is a good idea to pull back the spring and spring guide so that the ball-joint and
socket connection area is clear, to help in reattaching the ball-joint to the piston shaft.
SPRING
BALL-JOINT
EXTENSION
PISTON
SPRING GUIDE
SHAFT
FIGURE 9-3A REPLACING
THE LIQUID END (1)
FIGURE 9-3B REPLACING
THE LIQUID END (2)
FIGURE 9-3C REPLACING
THE LIQUID END (3)
2. Bring the liquid end and the control module together so that the ball-joint aligns with the
socket in the top of the piston shaft as shown in Figure 9-3B. Note the extension at the
end of the ball-joint – see arrow.
3. Carefully connect the ball-joint on the motor drive into the socket on the piston shaft. You
may need to maneuver the small extension downward to fully engage the two pieces,
shown connected in Figure 9-3C. Once the angle is correct, the ball and socket will join
together easily. DO NOT USE FORCE. Once the joint is made straighten the liquid end and
control module then release the spring and spring guide.
4. Hold the liquid end and control module together and thread the shaft coupling onto the
control module. Ensure the threads engage properly, and hand-tighten the shaft coupling
onto the control module.
5. Take the replacement encoder plug. Make sure the labels on
the plug and liquid end match.
6. Align the encoder plug with its slot in the head of the control
module (avoid touching the bottom of the plug) and insert the
plug squarely – see Figure 9-4. If power is supplied to the
EDP1 and the keyboard is unlocked, the initialization
sequence begins when the encoder plug is in place.
FIGURE 9-4 REPLACING
THE ENCODER PLUG
7. After the initialization sequence, look at the display. The EDP1 pipette should be in
PIPETTE mode with the display reading PICKUP. The nominal volume of the liquid end is
also displayed, as shown in Figure 9-5. (If your display does not show the proper
nominal volume, check that the correct encoder plug is installed.)
PICKUP
KB
1000µl
1000µl
FIGURE 9-5
DISPLAY AFTER INITIALIZATION SEQUENCE
(E1-1000 SHOWN)
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Care and Maintenance
10
EDP1 pipettes are designed to need very little maintenance and should give years of
trouble-free service if treated with proper care and the operating recommendations in this
manual are followed. Also see: RAININ Publication AB-15, Procedure for Evaluating
Accuracy and Precision of RAININ Pipettes, available on the Rainin website at
http://www.rainin.com/pdf/ab15.pdf
The following rules should be strictly observed to keep the mechanism dry and clean.
1. Never allow liquid to enter the shaft where it can contact the piston or seal.
2. Never pick up liquid without a disposable tip attached.
3. Never invert EDP1 or lay it on its side with liquid in the tip. Always hold it upright.
4. Never use solvents to clean EDP1. Instead, use a lint-free wipe dampened with water to
clean the instrument. Keep the keyboard and encoder plug dry.
SEALING
Periodically each liquid end should be tested to ensure proper sealing, as follows:
1. Set the nominal volume and attach a disposable tip.
2. Pick up the full volume of water.
3. Hold the instrument upright and observe the tip for 30 to 45 seconds.
There should be no leaks from the tip or droplet formation at the tip orifice. If leakage does
occur, sealing between the tip and the shaft may not be airtight.
SHAFT AND TIP SEALING
Use only RAININ disposable pipette tips. These tips mate exactly with the EDP1 liquid end
shaft to form airtight sealing, necessary for air-displacement pipettes to operate.
PISTON SEALING
Sealing between the piston and the inside surface of the shaft is performed by a PTFE seal
and a Buna O-ring. The PTFE seal extends through the O-ring, and only the PTFE seal
makes contact with the piston. The O-ring compresses the PTFE seal against the piston to
maintain proper sealing even after moderate wear, and also provides stationary sealing
between the PTFE seal and the shaft. Figure 10.1 shows a detailed view of the liquid end,
with each component separated for clarity.
PISTON ASSEMBLY
SPRING
FIGURE 10-1
SPRING GUIDE
O-RING
SEAL
PTFE SEAL
RETAINER
SHAFT
DETAIL VIEW OF EDP1 LIQUID END (200 µL SHOWN)
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A close-up detail of the 200 µL piston seal
assembly is shown in Figure 10-2: the seal retainer
can appear very different in other models.
PISTON
Periodically you should dismantle the liquid end for
inspection and cleaning, as follows:
SEAL RETAINER
PTFE SEAL
1. Remove the liquid end from the control module
as described in Section 9. Do not remove the
encoder plug for this procedure.
2. Pull the piston assembly out of the liquid end.
The PTFE seal and o-ring should stay attached to
the piston. If they remain in the liquid end, invert
the liquid end and gently tap it on the bench until
the seal assembly drops out.
O-RING
FIGURE 10-2 PISTON SEAL ASSEMBLY
(200 µL SHOWN)
3. Inspect the piston, PTFE seal and o-ring, seal retainer, and inside of the shaft for any dirt
and particulate contamination. Do not dismantle the piston assembly any further.
4. Remove any contamination by soaking and rinsing the piston assembly with distilled
water or alcohol. The piston surface should have a high-gloss finish. If the finish is dull,
scratched, or pitted, the PTFE seal may wear very rapidly, and the complete piston
assembly should be replaced.
5. Blow the assembly dry with clean dry compressed nitrogen, or dry it with a very soft lintfree wipe. To avoid scoring the piston, do not use rough material such as a paper towel.
CAUTION: Never apply grease to the piston or seal assembly (except on the
5 mL, 10 mL, and 20 mL models, which use a grease seal). For EDP1
pipettes with nominal volumes below 5 mL, any grease or lubricant may alter
the effective diameter of the piston and cause liquid measurement errors.
REASSEMBLY
1. Make sure the PTFE seal and o-ring are properly positioned on the piston.
2. Follow the procedure in Section 9 to replace the liquid end.
3. Attach a disposable tip, and test for proper sealing as described in the first part of this
chapter.
AUTOCLAVING
The autoclavable parts of EDP1 are the shaft and the tip ejector*: at a tempereature of
121°C, pressure of 1 bar, for a maximum duration of 15-20 minutes.
DO NOT AUTOCLAVE THE COMPLETE PIPETTE OR ANY PARTS OTHER THAN THE
SHAFT AND THE TIP EJECTOR.
* DO NOT AUTOCLAVE THE TIP EJECTOR ON THE 20 mL MODEL
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Troubleshooting
11
Table 11-1 Troubleshooting
Symptom
Possible Cause
Suggested Remedy*
Inaccurate liquid
measurement or leaks
Bad seal between disposable tip
and liquid end
Use RAININ disposable tips
Damaged shaft end
Replace shaft.
Inadequate seal on piston
assembly
Check, clean, or replace
piston seal.
“Lob” on display,
warbling alarm
Insufficient charge in Battery Pack
Recharge Battery
Pack; see Appendix B.
Blank display, no alarm
EDP1 is shut off
Press any top row key
or E to turn on instrument.
Depleted Battery Pack
Recharge Battery
Pack; see Appendix B.
Wall power supply disconnected
(when not using Battery Pack)
Reconnect cord to
EDP1 pipette.
Illegal volume entered
(will not be accepted)
Enter legal volume.
Display reads “Err”,
warbling alarm
Keyboard will not accept
Keyboard locked
entries, “KB locked” on LCD
Unlock keyboard by
pressing F 9.
* If the suggested remedy does not correct the problem, contact RAININ at 800-543-4030.
If anything confusing happens during operation of the EDP1 pipette, it can sometimes be
remedied by pressing F 0 (unless the display is flashing, indicating an entry must be
completed). Pressing F 0 returns the piston to HOME and activates the keyboard.
You can also reset the instrument by removing power, or removing and replacing the
encoder plug. (The keyboard MUST be unlocked if you remove the encoder plug.) The
pipette will restart and reinitialize. It will be in PIPETTE mode at its nominal volume after this
sequence. This remedy should be used only as a last resort, since all stored information will
be lost from the micro-computer and will need to be re-entered.
RAININ maintains service departments to correct any electro-mechanical problems with the
EDP1 pipette, or problems resulting from physical or chemical damage. In the U.S.A. please
contact Technical Service at 800-543-4030 for more information and assistance.
Outside the U.S.A. contact the RAININ distributor for your country. If you do not know the
address, call International Customer Service at 001-510-564-1614, or send e-mail to
[email protected]
RAININ distributors are also listed by country on the web: www.rainin-global.com/list.html
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Specifications
12
These manufacturer’s specifications should be used as guidelines when establishing your
own performance specification in accordance with ISO 8655.
EDP1 Model
10 µL
20 µL
100 µL
200 µL
300 µL
1000 µL
2000 µL
5000 µL
10 mL
20 mL
Volume
µL
Increment
µL
1
5
10
2
10
20
10
50
100
20
100
200
30
150
300
100
500
1000
200
1000
2000
500
2500
5000
1 mL
5 mL
10 mL
2 mL
10 mL
20 mL
0.01
0.02
0.1
0.2
0.2
1
2
10
10
20
Accuracy (Systematic Error)
%
µL (±)
2.5
1.5
1.0
7.5
1.5
1.0
3.5
0.8
0.8
2.5
0.8
0.8
2.5
0.8
0.8
3.0
0.8
0.8
3
0.8
0.8
2.4
0.6
0.6
5
1
0.8
5
1
0.8
0.025
0.075
0.1
0.15
0.15
0.2
0.35
0.4
0.8
0.5
0.8
1.6
0.75
1.2
2.4
3
4
8
6
8
16
12
15
30
50
50
80
100
100
160
Precision (Random Error)
%
µL (≤)
1.2
0.6
0.4
2.0
0.5
0.3
1.0
0.24
0.15
1
0.25
0.15
1
0.25
0.15
0.6
0.2
0.15
0.6
0.2
0.12
0.6
0.2
0.16
0.6
0.2
0.16
0.6
0.2
0.16
0.012
0.03
0.04
0.04
0.05
0.06
0.1
0.12
0.15
0.2
0.25
0.3
0.3
0.375
0.45
0.6
1
1.5
1.2
2
2.4
3
5
8
6
10
16
12
20
32
Specifications are subject to change without notice, and apply to operation in PIPETTE mode.
For ISO 8655 purposes, specifications can also be referred to as “Maximum Permissible Error”.
Electrical specifications
This device is intended for use only with the power sources with RAININ part numbers listed below. No
other power sources may be used with this device.
Wall Power Supply Input:
Wall Power Supply Output:
RCS Wall Transformer Supply Input:
6101-063
6100-064UX
6100-064EX
All P/Ns
100-130 VAC 50-60 Hz (US/JAPAN)
210-260 VAC, 50-60 Hz (UK)
210-260 VAC, 50-60 Hz (EU)
6 VDC 100 mA Regulated Nominal
6101-049
100-130 VAC 50-60 Hz (US/JAPAN)
6101-048UX
210-260 VAC, 50-60 Hz (UK)
6101-048EX
210-260 VAC, 50-60 Hz (EU)
RCS Wall Transformer Supply Output: All P/Ns
12 VAC 400 mA Regulated Nominal
Battery
6100-080 Ni-Cad 4.8 VDC Nominal 250 mAh Nominal
Explanation of symbols:
A - Ampere, Hz - Hertz, mAh - Milliamp Hour, VAC - Volts Alternating Current, VDC - Volts DIrect Current
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Recharging the Battery Pack
A
Power is supplied to the EDP1 by either a wall power supply cord, or with a Battery Pack. A
fully-charged Battery Pack will allow you to make up to 2000 full-stroke cycles before
charging is needed, depending on the pipette volume range. If the Battery Pack discharges
during operation, the EDP1 will sound a warning and the message “Lob” will be displayed.
If this occurs, connect the EDP1 to a charger immediately after the current pipetting cycle. If
you connect to the wall power supply, you can continue operation without interruption.
The Battery Pack can only be charged when installed in EDP1, in either of two ways:
WALL POWER SUPPLY
Connect the wall power supply cord to the connector on the back of the
control module. Connect the power supply to an AC power source with
the correct line voltage as specified on the power supply. The wall
power supply can recharge a completely discharged Battery Pack in
about 14 hours.
RAPID CHARGE STAND
Connect the Rapid Charge Stand to a suitable AC power source with
the correct voltage (see paragraph above). Place EDP1 on the stand
so that the four contact pins engage the contact slots on the stand. The
display reads “F C” (Fast Charge): functions are inoperative until EDP1
is removed from the stand. The Rapid Charge Stand can recharge a
Battery Pack in about 90 minutes. Current is metered precisely, and the
Battery Pack temperature continually monitored via a built-in sensor
probe. When the Battery Pack is fully charged, the red charge indicator
light on the Rapid Charge Stand goes out.
FIGURE A-1
CONNECTING
WALL POWER
SUPPLY
For extended storage of the EDP1, the Battery Pack should be fully
charged and removed from the instrument.
FIGURE A-2
RAPID CHARGE
STAND
ABOUT NICKEL-CADMIUM BATTERIES
The Battery Pack uses NiCad batteries, ideal for use in this application
because they are rechargeable with a long service life. However, all NiCads can develop
over time a “memory” or a propensity to seem fully charged at less than 100% of capacity.
This is a gradual process unlikely to be noticed in normal usage. In the extreme, the unit
could display “Lob” at increasingly frequent intervals after recharging for the requisite
period.
To extend the life of the Battery Pack, fully drain the batteries from time to time. The lowbattery alarm and the Lob display will alert you that it is time to recharge the batteries.
If you use a Rapid Charge Stand to rest the instrument, keep the Rapid Charge Stand
unplugged until you need to recharge the batteries. (Recharging takes about 1-1/2 hours
with the Rapid Charge Stand.)
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Ordering Information
B
EDP1 pipettes, parts and accessories can be ordered online 24/7 at www.rainin.com, or by
phone, mail, fax, or e-mail: 800-4-RAININ (800-472-4646) e-mail: [email protected]
Rainin Instrument, LLC.
7500 Edgewater Drive, Box 2160, Oakland, CA 94621-0060 • FAX 510-564-1617
Rainin Road, Box 4026, Woburn, MA 01888-4026 • FAX: 781-938-1152
See back page for other countries.
Complete EDP1 Pipettes: Includes one Control Module, one Wall Power Supply, and one
Liquid End for the nominal volumes listed below.
Cat. No.
Description
LTS MODELS
E1-10
E1-20
E1-100
E1-200
E1-300
E1-1000
E1-2000
E1-5000
E1-10ML
E1-20ML
TRADITIONAL MODELS
SE1-10
SE1-20
SE1-100
SE1-200
SE1-300
SE1-1000
SE1-2000
SE1-5000
SE1-10ML
n/a
E1-CM
Control Module:
Control Module only, for all volumes
LTS MODELS
E1-10LE
E1-20LE
E1-100LE
E1-200LE
E1-300LE
E1-1000LE
E1-2000LE
Interchangeable Liquid Ends: (Each is supplied with matching Encoder Plug)
TRADITIONAL MODELS
SE1-10LE
EDP1 Liquid End, 1-10 µL
SE1-20LE
EDP1 Liquid End, 2-20 µL
SE1-100LE
EDP1 Liquid End, 10-100 µL
SE1-200LE
EDP1 Liquid End, 20-200 µL
SE1-300LE
EDP1 Liquid End, 20-300 µL
SE1-1000LE
EDP1 Liquid End, 100-1000 µL
SE1-2000LE
EDP1 Liquid End, 200-2000 µL
EDP1 Electronic Pipette, 1-10 µL
EDP1 Electronic Pipette, 2-20 µL
EDP1 Electronic Pipette, 10-100 µL
EDP1 Electronic Pipette, 20-200 µL
EDP1 Electronic Pipette, 20-300 µL
EDP1 Electronic Pipette, 100-1000 µL
EDP1 Electronic Pipette, 200-2000 µL
EDP1 Electronic Pipette, 500-5000 µL
EDP1 Electronic Pipette, 1-10 mL
EDP1 Electronic Pipette, 2-20 mL
Replacement Battery Pack:
6100-080
Battery Pack for cordless operation.
(Rechargeable by Wall Power Supply or Rapid Charge Stand.)
6101-049
6101-048UX
6101-047EX
Rapid Charge Stand:
Rapid Charge Stand for EDP1 Pipette, US/Japan
Rapid Charge Stand for EDP1 Pipette, UK
Rapid Charge Stand for EDP1 Pipette, Europe
6100-063
6100-064UX
6100-044EX
Extra Wall Power Supply:
Wall Power Supply, 100-130V, 50-60Hz, US/Japan
Wall Power Supply, 210-260V, 50-60Hz, UK
Wall Power Supply, 210-260V, 50-60Hz, Europe
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Replacement Parts (Refer to drawings opposite)
A: Shaft Coupling
F: Spring Guide
B: Shaft
G: Seal Retainer
C: Tip Ejector D: Piston Assembly
H: Seal
J: O-ring
E: Spring
Parts for E3-Series 10 µL to 2000 µL:
A
B
C
D
E
F
G
H
J
E1-10
6107-063
6202-064
6202-071
6100-682
6200-195
6100-680
6200-196
6200-138
6200-139
E1-20
6107-063
6202-065
6202-071
6100-683
6200-197
6100-680
6200-198
6200-143
6200-170
E1-100
6107-063
6202-066
6202-073
6100-684
6200-197
6100-680
6200-201
6200-150
6200-151
E1-200
6107-063
6202-067
6200-156
6100-685
6200-199
6100-680
6200-200
6200-154
6200-155
E1-300
6107-063
6202-425
6202-419
6100-686
6200-199
6100-680
6200-416
6200-415
6200-414
E1-1000
6107-063
6202-068
6200-163
6100-687
6107-108
6100-680
6107-106
6200-161
6200-162
E1-2000
n/a
6202-214
6200-168
6100-688
6107-109
6100-681
6107-107
6200-166
6200-167
SE1-200
6107-063
6200-157
6200-156
6100-685
6200-199
6100-680
6200-200
6200-154
6200-155
SE1-300
6107-063
6200-413
6200-419
6100-686
6200-199
6100-680
6200-416
6200-415
6200-414
SE1-1000
6107-063
6200-160
6200-163
6100-687
6107-108
6100-680
6107-106
6200-161
6200-162
SE1-2000
n/a
6200-169
6200-168
6100-688
6107-109
6100-681
6107-107
6200-166
6200-167
Parts for SE1-Series 10 µL to 1000 µL:
A
B
C
D
E
F
G
H
J
SE1-10
6107-063
6200-140
6200-133
6100-682
6200-195
6100-680
6200-196
6200-138
6200-139
SE1-20
6107-063
6200-145
6200-144
6100-683
6200-197
6100-680
6200-198
6200-143
6200-170
SE1-100
6107-063
6200-147
6200-148
6100-684
6200-197
6100-680
6200-201
6200-150
6200-151
Parts for E1 and SE1 series 5000 µL to 10 mL:
A: Piston Assembly
B: Piston o-ring
D: Cylinder o-ring E: Shaft
F: Tip Ejector Arm
A
B
C
D
E E1 series
E SE1 series
F
5000 µL
6100-689
6107-112
6200-365
6200-364
6202-222
6200-362
6200-373
10 mL
6100-690
6107-113
6200-371
6200-370
6202-223
6200-368
6200-374
C: Cylinder
20 mL
6100-691
6202-299
6202-301
6202-300
6202-302
n/a
6202-298
Common parts for 5000 µL, 10 mL, and 20 mL E1 and SE1 series:
Tube of grease:
Filters for 5000 µL & 10 mL:
Filters for 20 mL:
6100-555
6190-164 (pkg of 100)
6190-221 (pkg of 100)
6190-165 (pkg of 1000)
6190-222 (pkg of 500)
FIGURE B-1 (OPPOSITE PAGE): LINE DRAWINGS OF EDP1 MODELS SHOWING REPLACEMENT PARTS
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EDP1 10 µL and 20 µL
EDP1 100 µL, 200 µL and 300 µL
D
D
E
E
F
F
G
A
A
H
J
G
H
J
B
B
C
C
Legend for EDP1 10 µL to 2000 µL Models
A: Shaft Coupling
B: Shaft
C: Tip Ejector
D: Piston Assembly
E: Spring
F: Spring Guide
G: Seal Retainer
H: Seal
J: O-ring
EDP1 1000 µL and 2000 µL
EDP1 5000 µL, 10 mL and 20 mL
A
D
E
B
F
Note: Tip ejector
arm shown is typical
for EDP1 5000 µL
and 10 mL.
E1-20ML has a
two-part tip ejector,
shown below.
G
H
A
C
D
E
B
F
C
Note: 2000 µL Model
does not use a shaft
coupling
Legend for EDP1 5000 µL, 10 mL and 20 mL Models
A: Piston Assembly B: Piston o-ring C: Cylinder
D: Cylinder o-ring
E: Shaft
F: Tip Ejector Arm
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Limited Warranty
See the enclosed Limited Warranty and Limitations of Liability Statement. Please complete and return the
Warranty Registration Card on receipt of your pipette.
RAININ pipettes are calibrated with RAININ tips. To assure excellent reproducibility and performance, use
only RAININ tips as recommended in this manual. Specified performance is guaranteed only when RAININ
tips are used.
Contacting RAININ
Direct Order Line: Phone: 800-472-4646
Fax:
510-564-1617
E-mail: [email protected]
Web:
www.rainin.com
RAININ Canada:
Phone: 800-472-4646
Fax: 866-758-3991
Pipette Service:
Phone: 800-662-7027
Fax:
510-564-1683
E-mail: [email protected]
Technical Support: Phone: 800-543-4030
Fax:
510-564-1617
E-mail: [email protected]
RAININ Japan:
Phone: ++ 81 3 5689-8311
Fax: ++ 81 3 5689-2670
METTLER TOLEDO GmbH, Switzerland
Phone: ++41 1 944 45 45
Fax:
++41 1 944 45 10
To call RAININ from outside the U.S.: 001-510-564-1600
For other International Offices and Distributors: See www.rainin-global.com
E-mail:
[email protected]
Web: www.rainin-global.com (from outside the U.S.A.)
EC Declaration of Conformity
according to ISO/IEC Guide 22 and EN45014
Manufacturer’s Name:
Rainin Instrument, LLC.
|Manufacturer’s Address: 7500 Edgewater Drive, Oakland, CA 94621
declares that the following product:
Product Name:
EDP1 Motorized Microliter Pipette
Model Number:
EDP1
Product Options:
Rapid Charge Stand, Wall Power Supply
conforms to the following Product Specifications:
Safety:
EN61010-1:2001
EMC:
FCC - 47 Part 15 Class A (Unintentional Signal Radiation)
IEC 61000-4-2, Edition 2.1 (2001) including Amds. 1&2 and EN 61000-4-2: Electrostatic Discharge
Immunity Test
IEC 61000-4-3, (2002) and EN 61000-4-3: Radiated Radio-Frequency Electromagnetic
Field Immunity Test
IEC 61000-4-4 (1995) + Amd. 1 (2000) & Amd.2 (2001 and EN 61000-4-4: Electrical Fast Transient /
Burst Immunity Test
IEC 61000-4-5 (1995) + Amd. 1(2000) and EN 61000-4-5: Surge Immunity Test
IEC 61000-4-6, Edition 2.0 (2003) and EN 61000-4-6: Immunity to Conducted Disturbances,
Induced by Radio-Frequency Fields
IEC 61000-4-8, Edition 1.1 (2001) and EN 61000-4-8: Power Frequency Magnetic Field Immunity Test
IEC 61000-4-11 (1994) + Amd.1 (2000) and EN 61000-4-11: Voltage Dips, Short Interruptions and
Voltage Variations Immunity Test
Supplementary Information:
Responsible Signatory:
Jim Petrek, VP of R&D
Date:
March, 2004
This Declaration of Conformity applies only to products which have the CE mark attached.
Rainin Instrument, LLC
7500 Edgewater Drive, Box 2160, Oakland, CA 94621-0060
Prices and specifications are subject to change without notice. Copyright 2004, Rainin Instrument, LLC.
a METTLER TOLEDO Company
9920-313 Rev A
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