NeoPrep Library System

NeoPrep Library System
Specification Sheet: Sequencing
NeoPrep™ Library Prep System
High-quality, reproducible NGS libraries with low input for all Illumina sequencing systems.
Highlights
• High Quality With Low Input
Generate high-quality, reproducible libraries, even from limited
starting material
• Sequencing-Ready Libraries With Unrivaled Simplicity
Prepare, quantify, and normalize in a single run*, optimized for
Illumina sequencing systems and informatics tools
• Versatile Library Prep for a Complete NGS Solution
Perform DNA and RNA assays, enabling complete and
seamless workflow solutions from one source
• Accessible System for Every Lab
Streamline laboratory infrastructure and reduce costs with any
lab, regardless of size, experience, or budget
*
Figure 1: NeoPrep Library Prep System—By combining the precision of digital
microfluidics and the simplest workflow in the industry, the NeoPrep System
delivers high-quality DNA and RNA libraries from low input amounts.
Sequencing-Ready Libraries, Simply Delivered
The NeoPrep Library Prep System (Figure 1) is a fully integrated
solution with a streamlined, easy-to-use workflow that produces
16 quantified and normalized sequencing-ready libraries per run*. This
seamless solution delivers high quality and reproducible results, even
with low input amounts of DNA or RNA.
Fully Integrated Workflow, Maximum Walk-Away
After loading fragmented DNA, the NeoPrep System performs all
remaining library preparation processes, including PCR amplification,
quantification, and normalization* (Figure 2). With pipetting time
dramatically reduced, users can walk-away for hours. From system
design to installation and operation, every aspect of the NeoPrep
System is optimized for efficiency and ease of use. An intuitive,
graphical user interface (Figure 3) quickly guides users through the run
setup in a few simple steps.
There are 3 simple steps in the NeoPrep workflow: run preparation,
library card loading, and library collection. The same workflow
prepares any type of library, including libraries that require complex
workflows when prepared manually, and needs just 30 minutes of
hands-on time.
Total
hands-on
time
Manual
DNA
Mechanical
Fragmentation*
NeoPrep
Mechanical
Fragmentation*
Size
selection
A-tailing
Ligation
Amplification
Quantification
Performed by NeoPrep System
Normalization
Recover
sequencingready libraries
~4
hours
Recover
sequencingready libraries
30
mins
*Requires Covaris Focused-ultrasonicator
Figure 2: The NeoPrep System Minimizes Hands-On Time—With built-in components for magnetic bead-based operations, thermal cycling, and optical detection,
the NeoPrep System enables users to walk-away from library prep.
* Quantification and normalization not currently available with TruSeq Stranded mRNA Kit-NeoPrep.
For Research Use Only. Not for use in diagnostic procedures.
Specification Sheet: Sequencing
A
Excellent Performance and Reproducibility
The NeoPrep System uses digital microfluidics technology to eliminate
almost all manual steps during library preparation, quantification, and
normalization*. This transformative technology precisely manipulates
droplets within the NeoPrep library card (Figure 4), reducing operator
variability, and human error.
B
Figure 4: NeoPrep Library Card—The 16-sample NeoPrep library card is
designed with a printed circuit board substrate and patterned electrodes to
facilitate droplet movement. Libraries are incubated, amplified, and washed
inside the card, saving users hours of hands-on time and significantly reducing
the potential for human errors.
High Quality With Low Input Amounts
The quality of NeoPrep libraries is comparable to that of libraries
prepared manually—but minimum input requirements for the NeoPrep
System are 4‑ to 10‑fold lower than those for similar manual methods,
making the system the ideal solution for users with limited starting
material (Figure 5).
C
Versatile Library Prep for a Range of
Applications
NeoPrep library prep kits include all necessary reagents and require
far fewer user-supplied consumables, such as plates and pipette tips,
reducing cost per sample. With fewer manual steps, quality control
processes are also significantly simplified (Table 2).
While these kits have been optimized to run out of the box, the system
allows users to modify some run parameters to fit their experimental
needs. For added scheduling flexibility, runs on the NeoPrep System
can be started at the end of the workday to run overnight. With run
times of ~7.5 hours for some assays, the NeoPrep System can run
twice a day, preparing up to 160 libraries per week. Because NeoPrep
workflows are the same from kit to kit, labs can quickly adopt new
library prep assays for a wide range of applications.
Figure 3: NeoPrep Run Setup—The graphical user interface quickly guides
users through a simple setup process that includes (A) selecting an assay, (B)
confirming the run settings, and (C) loading the library card.
* Quantification and normalization not currently available with TruSeq Stranded mRNA Kit-NeoPrep.
For Research Use Only. Not for use in diagnostic procedures.
Specification Sheet: Sequencing
Table 1: Lab Efficiency Comparison
Hands-On Time
NeoPrepa
100–1000 ng
25–100 ng
~4 hours
30 minutes
Pipetting Steps
40–60
< 10
Walk-away Time
Minimal
Full walk-away
Library prep,
quantification reagents
NeoPrep kit
Qubit, qPCR, or
RT-PCR instrument
N/A
Reagents
Library Quantification
Deviceb
Gene Abundance-FPKM
(15986 genes; R2 = 0.97)
A
100 ng - Manual
Input
Manual
a. Quantification and normalization not currently available with stranded mRNA.
b. Assay dependent
25 ng - NeoPrep
Simplicity, Speed, Support
NeoPrep System runs can be set up and monitored in BaseSpace®,
sequenced on any Illumina sequencing platform, and analyzed using
BaseSpace Apps. The complete, seamless workflow solution is
supported at every step of the process by the industry-leading Illumina
service team. With its unparalleled ease-of-use and competitive price,
the NeoPrep System allows any lab to prepare NGS libraries with
confidence and within budget.
Differentially Expressed Genes Detected
(15765 genes; R2 = 0.98)
25 ng - NeoPrep
B
Learn More
For more information and to learn which Illumina library prep kits are
currently available for the NeoPrep System, visit
www.illumina.com/neoprep.
Ordering Information
Product
Catalog No.
NeoPrep Library Prep System (installation included)
SE-601-1001
TruSeq Nano DNA Library Prep Kit for NeoPrep
(16 samples, 24 indexes)
NP-101-1001
TruSeq Stranded mRNA Library Prep Kit for NeoPrep
(16 samples, 24 indexes)
NP-202-1001
100 ng - Manual
Coverage of Challenging Regions, Human gDNA
(TruSeq Nano DNA 550 bp)
% Normalized Coverage
120
100
80
60
40
20
0
Genes
Exons ≥ 75% GC ≥ 85% GC AT DN
Manual 200 ng
NeoPrep 75 ng
Figure 5: NeoPrep and Manual Library Prep Methods—Libraries prepared
with the NeoPrep System show equivalent performance to comparable manual
methods using Illumina TruSeq® Nano DNA and TruSeq Stranded mRNA library
prep kits, with much lower input amounts.
* Quantification and normalization not currently available with TruSeq Stranded mRNA Kit-NeoPrep.
For Research Use Only. Not for use in diagnostic procedures.
Specification Sheet: Sequencing
NeoPrep System Performance Parameters
Input
gDNA or Total RNA
Input Quantityc
25–100 ng
Loading volume: 25–45 μl
Output
Quantified and normalized libraries
Library Yield
10–100 nM in 10 μl
Normalized Concentration
TruSeq Nano DNA Kit-NeoPrep: 10 nM
TruSeq Stranded mRNA Kit-NeoPrep: N/A
Throughput
16 libraries per run
Up to 80/160 libraries per week, depending on assay
Run Time
TruSeq Nano DNA Kit-NeoPrep: ~7.5 hoursd
TruSeq Stranded mRNA Kit-NeoPrep: ~9.5 hours (library prep only)e
Hands-On Time
30 minutes per run
Applications
Whole-genome sequencing, whole-transcriptome sequencing
See www.illumina.com/neoprep for a complete list.
Reagent Tracking
Via barcode scanner
c. Assay dependent.
d. NeoPrep System run time includes quantification and normalization. If only doing library prep, run time is reduced by ~2.5 hours.
e. Quantification and normalization not currently available with stranded mRNA.
NeoPrep System Specifications
Instrument Configuration
Integrated touch screen display
2D hand-held barcode scanner with USB cable
Instrument Controlf
Base unit: Intel Atom N450/D510 1.66 GHz CPU
Memory: 2 GB
Storage: 16 GB
Operating system: Windows embedded standard 7P
Operating Environment
Temperature: 19°C to 25°C (22°C ± 3°C)
Humidity: Noncondensing 20%–80% relative humidity
Altitude: Less than 2000 m (6500 ft)
Ventilation: Pollution degree II environment
For indoor use only
Dimensions
W×D×H: 51.3 cm × 41.4 cm × 34.3 cm (20.2 in × 16.3 in × 13.5 in)
Weight: 21 kg (~46 lbs)
Packaged weight: 30 kg (~67 lbs)
Power Requirements
100–240 VAC 50/60 Hz
Power consumption: 220 Watts
Product Safety and Compliance
NRTL certified IEC 61010-1
CE marked
Certified to the following standards:
UL STD 61010-1
CSA STD C22.2 No. 61010-1
EN/IEC 61010-1
EN/IEC 61326-1
EN/IEC 61326-2-6
Compliant with the following directives:
Low voltage directive 2006/95/EC
EMC Directive 2004/108/EC
f. Computer specifications are subject to change.
* Quantification and normalization not currently available with TruSeq Stranded mRNA Kit-NeoPrep.
Illumina • 1.800.809.4566 toll-free (US) • +1.858.202.4566 tel • [email protected] • www.illumina.com
For Research Use Only. Not for use in diagnostic procedures.
© 2014, 2015 Illumina, Inc. All rights reserved. Illumina, NeoPrep, TruSeq,and the pumpkin orange color are trademarks of Illumina, Inc. and/
or its affiliate(s) in the U.S. and/or other countries. Pub. No. 970-2014-004 Current as of 27 April 2016
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