Operation Manual Volume 2

Operation Manual Volume 2
Gas Chromatography (Volume 2)
Operation Manual
GC3420A
PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION
3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529
MRC.VER.01-8.13
[email protected]
CONTENTS
SECTION SIX COLUMNS......................................................................................... 1 SECTION SEVEN HELP. ................................................................................................... 40 SECTION EIGHT DISPLAYS .................................................................................. 89
SECTION NINE SPECIAL USES OF KEYS......................................................... 110
COLUMNS 1. INTRODUCTION
This section covers both packed column installation (para.3) and capillary column installation,
including MegaboreR columns, (Para. 2) For 3410 Series GC's.
Be sure to use the appropriate instructions for your hardware.
2. FUSED SILICA CAPILLARY COLUMN INSTALLATION
Proper column installation is required to ensure reproducible peak shapes without excessive tailing
due to absorption of analyte onto the polyimide coating and without noise due to graphite ferrule
particles being entrained by the fuel gases into the flame (for flame detectors).
The thin polymeric coating on fused silica columns will give some protection against breakage;
however, fused silica columns are somewhat fragile and must, be handled with care. Note: Do NOT
remove polymeric coating from the fused silica column.
2.1 Preparing Capi11ary Column Ends
To ensure no absorption of analyte onto the polyimide coating, the ends of the Column must be cut
squarely and smoothly. To achieve this proper scoring tool is required. A tungsten carbide pencil (P/N
29-900613-00) is recommended. A fine cut tool and die maker or jewelers file may also be used.
Note: Files with a coarser cut give rough edges and should not be used.
1.
VERY IMPORTANT NOTE: before cutting the sealed ends of the column, slide the
capillary column nut over the column end.
2.
Install the appropriate VespelR or
graphite ferrule onto the column with
the tapered edge toward the capillary
column nut, and the flat edge toward
the upper end of the column. See
Figure l.
This
procedure
prevents
contaminating the column with
ferrule material.
Refer to Tables l, 2, and 3 for common column ferrule part numbers.
1
2.1
Preparing Capillary Column Ends (cont.)
3.
Cut the column l to 2 cm from the sealed end by holding it securely between the thumb and
forefinger of one hand. Score the column once lightly with the cutting tool. See Figure 2.
4.
Grasp the column between thumb and forefinger. Per Figure 3, bend column slightly to break it at
the score mark. A magnifying lens (recommended 20X magnifier, P/N 00-997369-00) is necessary
to determine the quality of the cut (see Figure 3).
5.
If small splinters of silica or some of the outer coating remains on the column end, it is advisable
to make a fresh cut.
2
6.
2.2
Continue to the appropriate column connection paragraph for the injector installed.
Capillary Column Connection to Injector Fittings
Refer to the appropriate paragraph for connecting the column to your installed injector.
2.2.1 Capillary column Connection to the 1077 Split/Split less Capillary Injector
1.
The capillary column nut and the reversed ferrule should already be on the column per the
2.
procedure of paragraph 2.1. Note: the procedure detailed in paragraph 2.1 should be
followed to prevent column contamination. The position of the nut and ferrule are shown in
Figure l.
Uncoil about 20 cm of the injector end of the column.
3.
Move the column nut and ferrule to within 5 cm of the column end. Accurately measure 5.7 cm
from the end of the column and mark this distance with a felt pen. Do not allow the column
nut and ferrule to fall past this mark, as the marking medium may contaminate the
ferrule. Refer to Figure 4 for correct marking of column. Figure 4 is drawn to scale and may be
used for convenience to measure the proper distance.
4.
Partially insert the column into the lower end of the injector. Thread up the capillary column nut
and ferrule finger tight.
5.
Gently push the column up into the injector until the 5.7 cm mark on the column is aligned with
the bottom edge of the column nut. The column must go in the full 5.7 cm distance. If it does
not, the injector will not function properly.
6.
Continue to hold column as you tighten the column nut carefully and only enough to obtain a
good seal and hold the column firmly in place.
3
7. Continue to the appropriate column connection paragraph for the detector installed.
2.2.2 Capillary Column or MegaboreR column Connector to the l097/1098
1.
The capillary injector insert nut and the reversed ferrule should already be on the column per the
procedure of paragraph 2.1. Note:
The procedure detailed in paragraph 2.1 should be
followed to prevent column contamination. The position of the nut and ferrule are shown in
Figure l.
2.
Uncoil about 20 cm of the injector end of the column.
3.
Insert the syringe that will be used with the injector into the injector.
4.
Insert the column up into the injector. using the inserted needle as a guide. Gently push the
column fully up into the injector (approximately 3-1/2 inches), until the column comes to a firm
stop. This correctly positions the column against the upper end of the mega bore insert. The
column must go in the full distance; otherwise, the injector will not function properly.
5.
Continue to hold the column firmly in place as you tighten the column nut carefully and only
enough to hold the column firmly in place and obtain a good seal.
6.
After the column is installed at the injector end, make several dry injections to ensure that the
needle enters the column easily.
7.
Continue to the appropriate column connection paragraph for the detector installed.
4
FIGURE 4
REQUIRED COLUMN CONNECTION DIMENSIONS FOR DETECTORS AND THE 1077
SPLIT/SPLITLESS CAPILLARY INJECTOR
2.2.3 Capillary Column Connection to the 1090 Low Thermal Mass on Column Injector
1.
The capillary column nut and the reversed ferrule should already be on the column per the
procedure of paragraph 2.1. Note: The procedure detailed in paragraph 2.1 should be
followed to prevent column contamination. The position of the nut and ferrule are shown in
Figure l.
2.
3.
Uncoil about 20 cm of the injector end of the column.
Open the injector seal by rotating the injector nut counterclockwise (CCW) until it stops on the
injector nut stop (approximately l/2 turn).
5
4.
Carefully insert syringe into the injector until it comes to a full stop. The tip of the needle should
protrude beyond the injector body when viewed from the inside of the column oven.
5.
Slip the column over the protruding needle tip until the column comes to a full stop. This
correctly positions the column against the upper end of the alignment guide. The column must
go in the full distance. If it does not, the injector will not function properly.
6.
Thread up the capillary column nut and ferrule finger tight. Continue to hold the column firmly
in place as you tighten the column nut carefu11y and only enough to hold the Column firmly
in place and obtain a good seal.
7.
Continue to the appropriate column connection paragraph for the
2.3
Capillary Column Connection to Detector Fittings
1.
The capillary column nut and the reversed ferrule should already be on the column per the
procedure of paragraph 2.1. Note: The procedure detailed in paragraph 2.1 should be
followed to prevent column contamination. The position of the nut and ferrule are Shown in
Figure 1.
2.
Uncoil about 20 cm of the detector end of the Column.
3. Move the nut and ferrule to within 5 cm of the column end. The required distance for column
installation into the detector is 11.5 cm. See Figure 4. This is drawn to scale and may be used
for convenience to measure the proper distance.
Accurately measure the proper distance from the end of the column and mark this distance with
a felt pen. Do not allow the nut and ferrule to fall past this mark, as the marking medium
may contaminate the ferrule.
4.
Partially insert the column into the lower end of the detector. Thread up the capillary column nut
and ferrule finger tight.
5.
Gently push the column up into the detector until the distance marked on the column is aligned
with the bottom edge of the column nut.
The column must go in the full marked distance. If it does not, the detector will not function
properly. Ensure that the column length is within 2 mm of the required distance when installed.
6.
Continue to hold the column as you tighten the capillary column nut carefully and only enough
to obtain a good seal and hold the column firmly in place.
3
PACKED COLUMN INSTALLATION
6
Both glass and metal packed columns may be used with 34 Series GC's. Follow the appropriate
procedure for your column type.
3.1
Metal Column Installation
1.
Metal columns are usually installed with a metal two-piece ferrule in the detector column nut.
Properly orient the nut and ferrules on the detector column end per Figure 5. On the injector end,
thread the column nut, appropriate insert, and appropriate ferrule over the column in that order
only.
2.
Insert the column into both the injector and detector oven fittings and push up each end until the
column bottoms out in the injector and detector.
3.
Hold the column in place as you thread up the column nuts by hand. Avoid cross-threading.
4.
Using a small wrench, tighten the column nut on each fitting 3/4- turn past finger tight for 1/8"
columns or l-l/4-turns past finger tight for l/4" columns.
Follow the general rule l/4- to l/2- turn past fingertight for other than the initial seal.
7
3.2 Glass Column Installation
The fragile nature of glass requires additional care in handling during unpacking and installation.
Graphite, graphite VespelR, or VespelR ferrules are used in the column nut when installing glass
columns. NEVER use metal ferrules when installing glass columns.
1.
Properly orient the nut and ferrule on the
detector end per Figure 6. Before inserting the
column into the injector, install the appropriate
ferrules and inserts per the Injectors section of the
manual.
2. Slowly insert the column into both the injector
and detector oven fittings and gently push up each
end until the column just bottoms out in the
injector and detector.
3.
Hold the column in place as you thread up
the column nuts by hand. Avoid cross-threading.
4. Before a final tightening with a wrench, Withdraw each column end approximately l/32"from the
internal restriction. This prevents fracturing the end of the glass column when the nut is fully tightened
into place.
5.
Using a small open end wrench, tighten the column nut 3/4-turn past finger tight to achieve a
good seal. Tighten only enough to get a good gas tight seal. Over tightening can cause distorted
ferrules and possible broken columns.
Faults 495-496:
AC Power Control for a Heater or Coolant Valve is Stuck "Off"
See text for Faults 492-493.
8
Fault 495: Detector B Heater Off
Fault 496: Detector B Heater Intermittent
See text for Fault 491.
Faults 498-499:
Fault 500:
AC Power Control for a Heater or Coolant Valve is Stuck "Off"
Injector B
The inject switch is being held down for some reason. The GC will go back into run immediately at
the end of a run or if RESET is pushed. This condition is usually caused by over tightening the
injector nut Loosen it and see if anything else is holding the mechanism down.
If the fault remains when the switch actuator is completely free, there is a problem in the switch,
the injector harness, or the Temp. Control PCB, or the cable. Disconnect the switch from the harness.
If the fault goes away, replace the switch. Otherwise, disconnect the injector harness from the Temp.
Control PCB and install a spare Probe Simulator plug. (Note that all four connectors along the top of
the board must have harnesses or Probe Simulators on then.) If this cures the problem, replace the
injector harness. Otherwise, replace the Temp. Control PCB.
Temperatures cannot be measured or controlled accurately. Because of the hazard involved, all devices
operated directly from line voltage are disabled when these faults are detected. Operation can be
restored by pressing RESET to clear the fault display, but the instrument will shut down again if the
faults are detected in the background tests during normal operation.
Replace the Temperature Control PCB to correct any of these faults.
9
CONDITIONS:
ALL COVERS INSTALLED
ADJACENT THERMAL ZONES TURNED OFF
COLUMN MIXING FAN ON
20℃ AMBIENT
NO COLUMNS INSTALLED
LINE VOLTAGE: 115 VAC, 60Hz
TYPICAL BALLISTIC HEAT-UP AND COOL DOWN CURVES ( Figures 7, 8, 9, 10)
10
TABLE 2
SYMPTOM
1. Zone will not
heat; NOT
READY LED
on.
GENERAL THERMAL ZONE TROUBLE SHOOTING
POSSIBLE CAUSES
SOLUTION

Zone turned off in GC
configure table.

Reactive required zone.

Open heater, broken
terminals or unplugged
connector

Turn unit off, unplug from wall outlet,
and disconnect the indicate connector. If
the resistance for the following zones are
at room temperature and cannot be
measured, replace the heater or wiring.
The plugs numbers and resistance values
are:
For
typical
ballistic
heat-up and
cooldown
curves
for
various zones,
see
Figures
15-7 through
15-10
Column oven (1): P20, P21 pins1&2, 24 ohms;
Column oven (2): P20, P21 pins1&3, 24 ohms;
Universal Inj: P70, P72, or P79, pins1&2, 112
ohms;
OCI (120 VAC): P70, P72, or P79, pins1&2, 70
ohms;
OCI (240 VAC): P70, P72, or P79, pins1&2,
280 ohms;
Ion detector base (120 VAC): P71, pin 1&2, 65
ohms
Ion detector base (240 VAC): P71, pin 1&4,
260 ohms
TCD block (1): P71, P78, pins 1&2 287 ohms
TCD block (2): P71, P78, pins 3&4 287 ohms
Replacement column oven heater is
03-917096-00
2.
Erratic
control,
cycling display

Bad ADC on temperature
control PCB or bad
temperature
sensing
probe.


Temperature
probe
not
positioned
sensing
correctly


Heater and sensor lead,
lugs,
or
connectors
deteriorated, loose, or
dirty


Thermal

leak
with
11
Replace temp. sensor probe plug with
probe simulator. Display temp. for zone
should be 0±15℃. If not, replace temp.
control PCB. If reading =zero, probe
may be faulty.
Check the probe positions per figures in
the appropriate detector/injector section
column oven probe body must be
exposed at least 3/4 inside column oven.
Unplugging
and
replugging
the
connector may be sufficient to wipe
contacts clean. Any heater terminal that
is discolored is an indication of a poor
connection and needs prompt repair.
During reconfiguration or repair,
neighboring
ambient
3. Subambient
setpoint
not
obtainable
( column
oven and
on-column
capillary
injector
oven only)

First, check
possible
causes from
symptoms 1
and
2,
above.








zone
or
insulating material may have been left
out or overly compressed.
Coolant
time-out
occurred
in
GC
Configure table
Setpoint is below limit
for coolant: max. limit
for LCO2 is -55℃, LN2
is -99℃
LN2 feed tube poorly
installed.

Reactive zone.

Recheck method for setpoint and coolant
to be used.

Keep line as short as possible and well
insulted. LN2 has to cool the feed tube
before it can cool the oven.
Check all valves and replace tank, if
empty.
Clean line and nozzle if fog is absent.
Check only when oven is near ambient
temperature. A heavy fog is an
indication of normal operation.
Replace valve if no audible click is
heard.
Gas source depleted or
tank shut off.
Feed line or nozzle
restricted.

Control valve faulty.
Notice if valve has an
audible
click
when
setpoint changed to a
value above and below
actual temperature.
Vent not fully closed.
Insulation iced up around
nozzle.

Wrong coolant selection
in configuration table.




See table 3, symptom number 2.
This is usually caused by opening the
column oven door when the oven is at
subambient temperatures, which causes
condensation to occur and ice to form.
Select either injector or column oven for
control, as required.
TABLE 3 COLUMN OVEN TROUBLE SHOOTING
SYMPTOM
1 Column oven
won’t
program per
spec. in
Figure8.
POSSIBLE CAUSE
SOLUTION

Mix fan faulty


Fan blade loose.

12
Check for blade rotating.
a. if blade not rotating, move blade
manually. If rotation is obstructed, remove
object.
b. if blade can’t be moved manually,
bearings have sized. Replaced motor.
c. if blade can be rotated manually, the
motor or its leads are open. Replace motor.
Observe blade for wobble or shaft rotating

One of the two heaters
or its lead is open.




Vent motor or switches
faulty.
Vent
flap
is
malfunctioning.
Triac Q1 is faulty.

Mix fan faulty


Fan blade loose.


Vent motor cycling or
erratic.
Cooling fan faulty.

Check for blade rotating.
a. if blade not rotating, move blade
manually. If rotation is obstructed, remove
object.
b. if blade can’t be moved manually,
bearings have sized. Replaced motor.
c. if blade can be rotated manually, the
motor or its leads are open. Replace motor.
Observe blade for wobble or shaft rotating
and blade remaining stationary. Tighten
blade setscrews.
Do the vent test, para. 5.2.

Replace fan if not rotating.

2 Erratic
operation
and blade remaining stationary. Tighten
blade setscrews.
Disconnect P20 &P21 and measure about
24 ohms between pins 1 and 2, and pin 1
and 3. if the value is not measured, replace
the heaters or wiring.
Do the vent test, para. 5.2.



Broken/bent vent flaps need to be replaced
if they are not flush with the oven.
If Triac Q1 is bad, it will require replacing
the mother PCB. Recheck the Temperature
Control PCB, wiring, heaters, ect., as
replacement of the Mother PCB is
extensive task and should be left as a last
restort.
TABLE 4 UNIVERSAL INJECTOR/AUXILIARY OVEN TROUBLESHOOTING
SYMPTOM
POSSIBLE CAUSES
SOLUTION
1 Injector will not
heat up to set point
per spec in Figure
Review para. 8.1
Two halves of injector clam-shell must be
tightly clamped with mounting hardware.
2 Auxiliary oven not
control properly.
Variable, due to this
being a user-designed
heat zone. Refer to
para. 8.1 for typical
symptom
and
solutions.
This user-designed heat zone requires a close
thermal coupling between heater and probe and
correct heater size to load. Also, make certain
that a 100 ohm at 0℃.0.00392/ohm/ohm/℃
type of platinum probe is used.
13
TABLE 5 DETECTOR OVEN
SYMPTOM
1 Ion or TCD oven will
not heat to upper
temperatures and heats
slowly per spec. in
Figure 7 (ion) or
Figure 9 (TCD)
8.5
POSSIBLE CAUSES
SOLUTION

Review para. 8.1


One of the two
heaters or its lead
is open

Make certain that both heaters and the
probe are installed correctly. See
appropriate figure in section8, 9, 11, or
12 for ionization heaters and probe.
For TCD measure P71 resistance of
287 ohms between pin 1 and 2 and pin
3 and 4. if this value is not measured,
replace the heaters or wiring.
External Events/Valve Control
This information is provided to help find faults in External Events/-Valve Control that built-in
diagnostics do not cover. This information assumes that you have run the Automatic Tests and the
fix tended Tests for External Events, no faults have been reported, but you know that you have a
problem.
If you do not feel comfortable using an ohmmeter and doing minor repairs or have access to a
person that is, there are two courses of action. The first is to replace the External Events PCB from
a spares kit; the second is to call the local dealer.
If you do not have an External Events PCB as a spare or you have replaced the PCB and the
problem remains, proceed with Table 8.
TABLE 8 EXTERNAL EVENTS TROUBLESHOOTING
SYMPTOM
1 Valve or
solenoid
does not
operate
POSSIBLE CAUSES







2 Channel
select

SOLUTION
RELAY section not in active
method
Valve or solenoid is not turned
on in RELAY section
Valve or solenoid is connected
to wrong event
Screws clamping valve or
solenoid wires are not screwed
down on TB1 on the External
Events PCB
Valve
or
solenoid
not
connected
Broken wire on pcb
Valve or solenoid faulty

Add RELAY section


Add a timed event to RELAY
section
Change RELAY section or move
valve or solenoid or proper event
Secure screws

Connect valve or solenoid


Repair pcb
Replace defective valve or solenoid
RELAY section not in active
method

Add a program for relay 3
14

does not
work
No control for relay 3 in
RELAY section
Channel select cables not
connected properly
Cables bad

Add a program for relay 3


External events pcb faulty

See external events installation in
the External Event section
Visually
inspect,
and
check
continuity with and ohmmeter.
Removing and reconnecting may be
sufficient cleaning if connections
were oxidized. Exchange is the best
method
of
localizing
cable
problems.
Replace pcb


Add a program for relay 4

Add a program for relay 4

Install cable

RELAY section not in active
section
No control for relay 4 in
RELAY section
Cable to auxiliary connection
J96 missing or not connected
Cable bad


External events PCB faulty.

Visually
inspect,
and
check
continuity with and ohmmeter.
Removing and reconnecting may be
sufficient cleaning if connections
were oxidized. Exchange is the best
method
of
localizing
cable
problems.
Replace pcb



3
Auxiliary
relay
does
not work



8.6 printer/plotter
Refer to Table 9 for Printer/Plotter Troubleshooting
TABLE 9 PRINTER/PLOTTER TROUBLESHOOTING
SYMPTOM
1 PLOTTER
section cannot
be built into
the method
POSSIBLE CAUSES
SOLUTION

The GC has a fault


Printer/plotter unplugged


Pringer/plotter fuse blown

15
Do the automatic tests per
para. 1.2
Remove the screw and slide
the printer/plotter out and
check that the flat ribbon
cable is connected to J61 and
that the power plug is
connected to J69. check for
loose connector.
While under power, observe if
both green LED’s are lit. if
not, replace fuse F 1.
2 Paper-out false
indication
3
4
Paper feeds
erratically,
winkles, and/or
jams
Distorted
printing or
plotting

Flat cable faulty or bad connector


Printer/plotter faulty


Torn paper


Paper out
unplugged

Torn paper from the supplier



Use of non-standard paper
Paper stored improperly




GC front panel door not shut
GC faulty or erratic



Printer/plotter erratic or faulty


GC faulty


Paper feed erratic


Loose belt on paper feed motor or
thermal printhead motor
Head control erratic


sensor
faulty
16
or


If both LED’s are lit, the flat
cable need replacing.
If the status LED is off, the
printer/plotter is faulty and
need replacing.
Observe the web of the paper
from the roller is untorn and
feeding straight.
If the sensor is connected to
J66, disconnect and inspect
connector. (unplugging and
reconnecting may sufficiently
clean the contacts.) if no fault
is
found,
replace
the
printer/plotter unit.
Take-out the paper and
re-feed
it
again,
per
instruction in the Quick
Reference Manual
Use only standard paper
If the newly installed paper
has been stored in an
excessively cool and humid
location, it may take several
days for it to feed correctly in
the lab environment.
Shut GC door
Do the Automatic Tests,
para.1.2
Replace printer/plotter
Do the Automatic Tests,
para.1.2
Check distance between time
ticks. If not correct, check for
jammed paper. See symptom
#3. Press [SHIFT][FEED
PAPER]. If paper doesn’t feed
smoothly,
Tighten the belt
If the head can move freely,
and pieces of paper are
obstructing its travel replace
the printer/plotter.
5 Print or plot is
light
6 Missing dots


Use of non-standard paper
Switch S1 on Printer/Plotter Power
Supply PCB in wrong position.



Residue build up on printhead


Pinter, power supply, or printhead
faulty.

Use only standard paper
Each printhead is selected (A,
B or C) and stamped
accordingly on the head cable.
Set S1 on the printer/plotter
Power
Supply
PCB
accordingly.
Remove the paper, clean the
head with an alcohol soaked
non-abrasive pad.
Replace printer/plotter.

Printhead or PCL/ADC PC Board
faulty

Replace printer/plotter.
8.8 Misce11aneous Troubleshooting
Refer to Table 11 for Misce11aneous Troub1eshooting, which includes inject switch, remote
control, and igniter.
TABLE 11 MISCELLANEOUS TROUBLE SHOOTING
SYMPTOM
POSSIBLE CAUSE
1 Instrument won’t
go to RUN after
injection, either
with
Autosampler or
manual injection


2
Flame won’t
ignite on FID or
FPD
SOLUTION
Controller faulty
Inject switch, wiring, or
connector faulty.
Inject switch bent or
mechnically obstructed.


Run Automatic Tests, para. 1.2
Injection switch may be stuck open
or closed.
(a) Inject stuck down or shorted
wiring will allow one run and stop
all other. Check for error message.
(b) Inject switch stuck up or broke
wiring will prevent any run from
starting.

Keyboard
display,
temperature control, or
Power Supply PCB faulty.


Faulty ignition coil, cable,
or connector at the tower
on the pc board

Run Automatic Tests, para. 1.2. Key
should “beep” when depressed.
Check to see if “beep” has been
disabled in Configuration Section.
With H2 flow turned off, press and
hold IGNITE and observe ignitor
coil inside the appropriate detector
tower. The coil should glow red.
Check that the connectors are both
secure and are connected to correct
pc board. Check continuity of the
cable or coil with an ohmmeter or
use replacements to localize fault.

Ignitor probe assembly

17

3 Remote start or
Ready
inputs
control
signals
faulty.
4 Remote start or
Ready
output
signal faulty
5
TCD
has
background Fault
43/53, even under
normal conditions
with no leaks.
not installed properly.
No fuel gas at tower.


Improperly adjusted flow
rates.


Faulty cable
connector
its


Faulty temperature control
PCB board


Faulty cable
connector
its


Faulty Keyboard Display
PCB board


Badly oxidized cell.

or
or
Ferrule may be over tightened. Gas
supply may be depleted or shut off.
Adjust flow rates per the Installation
section.
Check quality of cable and
connectors, preferably with an
ohmmeter. Since these control
signals are basic, it is easy to
simulate the remote station; short
pins 1 and 2 on J16 to give a
READY condition; short pins 3 and
4 on J16 to give a START condition.
Exchange the pc board
Check quality of cable and
connectors, preferably with an
ohmmeter. These control signal can
be measured with an ohmmeter.
Measure a short pins 1 and 3 on J23
to for a READY instrument, open
for NOT READY. Measure a 1
second short at the start of a run on
pins 4 and 5 on J23.
Exchange the Keyboard Display
PCB. DO NOT forget to replace the
metal cover after the exchange.
Switch to 490 position on Switch S1
on the TCD PCB. Replace the cell if
that does not work.
8.9 Pressure Transducer
This information is provided to help find faults in the Pressure Transducer option that built-in
diagnostic do not cover. This information assumes that you have run the Automatic Tests, no faults
have been reported, but you know that a problem exists.
The Pressure Transducer option consists of a Pressure Transducer PC Board mounted in the
pneumatics compartment, interface circuitry always present on an External Events.
Events PCB, and a cable going between the two pcb’s.
If you do not feel comfortable using an ohmmeter and doing minor repairs or have access to a
18
person that is, there are two courses of action. The first is to replace the External Events or Auto
Sampler/External Events PCB from a spares kit; the second is to call the local dealer.
If you do not have a External Events
Events PCB as a spare, or you have replaced the PCB and the problem remains, then proceed with
Table 12, Pressure Transducer Troubleshooting.
0
SOLUTION PROCEDURES
8.9.1
(Ref. Symptom l, Table 12)
Turn instrument off, unplug from main power, and remove the high voltage cover. Remove the
External Events PCB. Remove the Pressure Transducer cable (03-917829-00).
From J103 0n the External Events PCB. Connect an ohmmeter between pins 9 and 2 on P103.
If the ohmmeter reads zero ohms, then the External Events PCB is faulty and should be replaced.
If the ohmmeter reads infinity, then either the Pressure Transducer cable or the Pressure
Transducer PCB is faulty. The most likely source of a problem is the cable.
8.9.2
(Ref. Symptom 2, Table 12)
Turn instrument off, unplug from main power, and remove the high voltage cover. Remove the
External Events PCB. Remove the Pressure Transducer cable (03-917829-00) from J103 on the
External Events PCB. Connect an ohmmeter between pins 2 and 10 on P103. If the ohmmeter
reads infinity, then the Pressure Transducer cable and Pressure. Transducer PCB are okay. Replace
the External Events PCB.
If the ohmmeter reads zero, then either the cable or the Pressure Transducer PC Board is faulty.
The most likely source is the Pressure Transducer PC Board.
8.9.3
(Ref. Symptom 3, Table 12)
If the change in pressure reading is real (not electronic), then there should also be a change in the
chromatogram. If no chromatogram is available, inject a known sample under known instrument
conditions. If the chromatogram has changed, go to the chromatography troubleshooting section
(Table 14) of this troubleshooting manual. If there has been no change in the chromatogram, then
there is an electronic problem. Go to Para. 8.9.4.
8.9.4
(Ref. Symptom 3, Table 12)
To locate the fault down to the level of a replaceable assembly will require a higher degree of
troubleshooting by the operator than has previously been assumed. The most likely assembly to
have failed is the Pressure Transducer PCB. The operator may choose to replace the Pressure
Transducer PCB and see if the new assembly fixes the problem. The operator may choose to call a
19
serviceman at this point.
The procedure that follows requires the use of a digital millimeter and a pressure gauge of known
accuracy "T'd" into the pneumatic line going to the Pressure Transducer. If these are not available,
then do not proceed further.
1.
2.
Perform the calibration described in the Installation section of this manual. If the
Pressure Transducer voltages are within the accuracy of the pressure gauge you are
using, the Pressure Transducer PCB is okay. Go to step 2. If the Pressure Transducer
does not pass the calibration, replace the Pressure Transducer PCB.
Disconnect the Pressure Transducer cable from the Pressure Transducer PCB and the
External Events PCB. Measure the resistance from pin 5 at one end of the cable to pin 5
at the other end of the cable. Also measure the resistance from pin 6 at one end of the
cable to pin 6 at the other end of the cable. Both wires should measure zero ohms. If
they do not, the cable is defective and should be replaced. If both wires do measure zero
ohms, the External Events PCB is probably defective and should be replaced.
TABLE 12 PRESSURE TRANSDUCER TROUBLESHOOTING
SYMPTOM
POSSIBLE CAUSES
SOLUTION

Option not present.


GC Configure table states column(s)
not installed (COLUMN A/B
INSTALLED? = NO)
External Event PCB board lock not
closed.
Option not connected properly.


Faulty External Event PCB, pressure
Transducer cable, or Pressure
Transducer PCB.

See Installation section of
this manual for pressure
transducer installation.
See para.8.9.1.
Pressure for
either A or B
not reported in
Status or Run
log

Faulty
E/E
PCB,
Pressure
Transducer cable, or Pressure
Transducer PCB.
GC Configure table states column(s)
not installed (COLUMN A/B
INSTALLED? = NO)

See para.8.9.2.

In GC Configure table,
enter YES for COLUMN
A/B INSTALLED.
3 Channel A or
channel
B
pressure
readings
are


Carrier gas depleted or shut off.
Faulty External Event PCB, pressure
Transducer cable, or Pressure
Transducer PCB.


See para.8.9.3.
See para.8.9.4.
1 Both A and B
pressure
not
reported
in
instrument
Status or Run
log


2

20


Option
requires
the
presence of a Pressure
Transducer PCB, cable and
External Event option.
In GC Configure table,
enter YES for COLUMN
A/B INSTALLED.
Close board lock.
wrong

Pressure Transducer outputs are out
of allowed range.

Measure votages between
the following test points on
the Pressure with the
instrument power on.
TP1 and TP3 – allowed
range = 0 to +5 volts

8.10
Pressure
calibration.
Transducer
needs

TP2 and TP3 – allowed
range = 0 to +5 volts
If both measurements are
good, then check the
pressure display on the
instrument.
See the Installation section
of this manual.
Split Ratio Transducer
This information is provided to help find faults in the Split Ratio Transducer option that built-in
diagnostic do not cover. This information assumes that you have run the Automatic Tests, no faults
have been reported, but you know that a problem exists.
The Split Ratio Transducer option consists of a Split Ratio Transducer PC Board mounted in the
pneumatics compartment, interface circuitry always present on an External Events or
AutoSampler/External Events PCB, and a cable going between the two pcb's.
If you do not feel comfortable using an ohmmeter and doing minor repairs or have access to a
person that is, there are two courses of action. The first is to replace the External Events.
Events PCB from a spares kit; the second is to call local dealer. If you do not have a External
Events.
Events PCB as a spare, or you have replaced the PCB and the problem remains, then proceed with
Table 13, Split Ratio Transducer Troubleshooting.
o
SOLUTION PROCEDURES
8.10.1 (Ref. Symptom l. Table 13)
Turn instrument off, unplug from main power, and remove the high voltage cover. Remove the
External Events PCB. Remove the Flow Transducer cable from
J102 on the External Events PCB. Connect an ohmmeter between pins 7 and 2 0n P102.
If the ohmmeter reads zero ohms, then the External Events PCB is faulty and should be replaced.
If the ohmmeter reads infinity, then either the Flow Transducer cable or the Split Ratio Transducer
21
PCB is faulty. The most likely source of a problem is the cable.
8.10.2
(Ref. Symptom 2, Table 13)
Turn instrument off, unplug from main power, and remove the high voltage cover. Remove the
External Events PCB. Remove the Flow Transducer cable from J102 0n the External Events PCB.
Connect an ohmmeter between pins 2 and 8 0n P102. If the ohmmeter reads infinity, then the Flow
Transducer cable and Pressure Transducer PCB are okay. Replace the External Events PCB.
If the ohmmeter reads zero, then either the cable or the Flow Transducer cable is faulty. The most
likely source is the Split Ratio Transducer.
8.10.3
(Ref. Symptom 3, Table 13)
If the change in split ratio reading is real (not electronic), then there should also be a change in the
chromatogram. If no chromatogram is available, inject a known sample under known instrument
conditions. If the chromatogram has changed, go to the chromatography troubleshooting section
(Table 14) of this troubleshooting manual. If there has been no change in the chromatogram, then
there is an electronic problem. Go to Para. 8.10.4.
8.10.4 (Ref. Symptom 3, Table 13)
To locate the fault down to the level of a replaceable assembly will require a higher degree of
troubleshooting by the operator than has previously been assumed. The most likely assembly to
have failed is the Split Ratio Transducer PCB. The operator may choose to replace the Split Ratio
Transducer PCB and see if the new assembly fixes the problem. The operator may choose to call a
serviceman at this point.
The procedure that follows requires the use of a digital millimeter and a bubble flow meter
attached to the splitter exit on the left side of the GC. If these are not available, then do not
proceed further.
1.
Perform the calibration described in the Installation section of this manual. If the Split
Ratio Transducer voltages are within the accuracy of the flow meter gauge you are using, the Split
Ratio Transducer PCB is okay. Go to step 2. If the Split Ratio Transducer does not pass the
calibration, replace the Split Ratio Transducer PCB.
2.
Disconnect the Flow Transducer cable from the Split Ratio Transducer PCB and the External
Events PCB. Measure the resistance from pin 5 at one end (P1ll) of the cable to pin4 at the
other end (P102) of the cable. Also measure the resistance from pin 6 at one end (Plll) of the cable
to pin 6 at the other end (P102) of the cable. Both wires should measure zero ohms. If they do
not, the cable is defective and should be replaced. If both wires do measure zero ohms, the
External Events PCB is probably defective and should be replaced.
22
TABLE 13 SPLIT RATIO TRANSDUCER TROUBLESHOOTING
SYMPTOM
1 Both A and B split ratio not
reported
in
instrument
Status or Run log
POSSIBLE CAUSE

Option not present.


GC Configure table states
column(s) not installed
(COLUMN
A/B
INSTALLED? = NO)
External Event PCB
board lock not closed.
Option not connected
properly.





2 Split ratio for either A or B
not reported in Status or
Run log

3 Channel A or channel B split
ratio readings are wrong
SOLUTION



Option
requires
the
presence of a Split Ratio
Transducer PCB, cable
and
External
Event
option.
In GC Configure table,
enter YES for COLUMN
A/B INSTALLED.

Close board lock.

See Installation section of
this manual for split ratio
transducer installation.
Faulty External Event
PCB, Flow Transducer
cable, or Split Ratio
Transducer PCB.

See para.8.10.1.
Faulty E/E PCB, Flow
Transducer cable, or Split
Ratio Transducer PCB.
GC Configure table states
column(s) not installed
(COLUMN
A/B
INSTALLED? = NO)

See para.8.10.2.

In GC Configure table,
enter YES for COLUMN
A/B INSTALLED.
Carrier gas depleted or
shut off.
Faulty External Event
PCB,
split
ratio
Transducer cable, or split
ratio Transducer PCB.
Outputs of channel A or B
on the Split Ratio
Transducer are out of
allowed range.

See para.8.10.3.

See para.8.10.4.

With the instrument
turned on, check with a
voltmeter the voltage
between the following
test points:
Split Ratio Transducer
PCB:
TP1 or TP5 and TP3 or
TP6 (grounds)
TP2 or TP4 and TP3 or
23


8.11
Outputs of the Pressure
Transducer may be out of
the allowed range.
The Pressure Transducer
must be calibration before
the Split Ratio Transducer
can
be
accurately
calibrated.


TP6 (grounds)
If any of the voltage
measurements are not
within 0 to 5 volts, then
the channel/side with this
bad measurement should
be recalibrated or it may
be defective. First, see
split ratio transducer
calibration in the
Installation section
See table 12, Pressure
Transducer
Troubleshooting.
Refer to the installation
section for either pressure
or split ratio transducer
calibration.
Chromatographic Interpretations
The troubleshooting procedures in this section are organized based on observable chromatographic
deviations from known responses of a standard sample. This may be any combination of peak
shapes, area, elution times or baseline characteristics. Because these observations may be the only
indication of a potential problem, the fault can be very elusive and may be anywhere in the system
and yet appear to be very obviously the fault of something else. Because of the complexity of
localizing a chromatographic problem, always recheck your method, the hardware, and perform
the Automatic Tests per paragraph l.2. All discussions assume a basic GC and recorder
configuration.
The following sections deal with observations. Study your data, determine what it should be, and
review the following list for the best match. The solutions in these sections assume that the
operator has some knowledge of chromatographic operation and maintenance. Refresh yourself
with the paragraphs dealing with split ratio and pressure transducer calibration in the Installation
section.
Refer to Table 14 for Chromatographic Interpretations Troubleshooting.
Symptom #
1
2
3
4
5
Symptom Name
Changes in sensitivity; peak missing or very small
Tailing Peaks.
Changes in retention time.
Increased noise level (with negligible drift)
Constant baseline drift in one direction
24
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
Cycling baseline
Spikes at irregular intervals
Short spikes or peaks at regular intervals
Rising baseline when temperature programming
Baseline stepping. Baseline does not return to zero,
Attenuation is incorrect, peaks are flat-topped.
Baseline cannot be zeroed
Unexpected peaks
Unresolved peaks
Square-topped peaks
Round-topped peaks
Negative peaks
Extra peaks
FID only--Sudden drop-off of otherwise normal peak.
Pen returns to level below previous baseline. FID
Flame becomes extinguished.
Negative dips after peaks
Flash injection only--Loss of resolution.
Poor resolution (between peaks l and 2).
Leading peaks
Reverse leading peaks
TABLE 14 CHROMATOGRAPHIC TROUBLESHOOTING
SYMPTOM
POSSIBLE CAUSES
SOLUTION

A.
check
that the recorder power

Chart
recorder
or
1 Change in sensitivity
cord is plugged into wall outlet of
printer/plotter
(No or lower peak
proper voltage and into the rear of
inoperative
or
faulty
height) with no other
the recorder. Exchange with a
deviations.
known good chart recorder
“Sensitivity ”refers to
B. for the built-in printer/plotter,
peak size (height or
perform Automatic Tests per
area) for a certain
para.1.2.
amount
of
sample  Recorder or plotter  Check method or attenuator knob
injected.
setting on recorder.
attenuation or misset
 Recorder cables faulty  Check singal cables:
a. check plug position at GC and
or mis-connected
recorder
b. make sure recorder terminals
don’t have a shorting strip or
plug installed
 Chart recorder zero  Check recorder zero button to
make sure that it is not stuck or
button faulty
hold down or faulty.
 Detector
cables,  Visually inspect the detector:
a. See para.7 for lists of
connectors & related
diagrams
of
detector
internal parts faulty or
Since the retention
assemblied.
miss- assembled
times are constant the
b. Internal tower parts can be
change in sensitivity
contaminated, depending on
could not be due to a
25
change in column flow
rate.
Most likely causes are
associated with either
the sample not being
delivered to the detector
or a sub par performing
detector:
 Leak in the syringe
 Leak in the septum
during injection
 Plugged syringe
 Lead at column
exit
 Contaminated
detector

Autosampler faulty


Column
switching
valves leaking


Make-up, carrier, or fuel
gas flows wrong


Flame out lit (FID,
FPD)
TCD out of balance or
faulty filament
Septum leaking


Syringe (manual
A/S) faulty
and


TCD
filament
protection fuse F2 open


ECD electrometer
pulser bad
or


Sample reaction with
system components.


Column contaminated.



2 Tailing peaks or
unsymmetrical peak
slopes.
This peak has a
normal leading slope
and partial normal back
slope, but before
26


analysis.
Clean
and
reassemble collectors, tips,
cells etc.
c. Cables at tower (ion only) and
pcb connector detents to be
fully seated.
d. If a dual detector is installed,
exchange cables to assist in
isolating the fault.
See autosampler troubleshooting,
table 6 (8034 A/S) or table 7
(8035 A/S).
Check for leaks and proper
switching operation. See the
Installation section for pneumatic
leak checking.
Check flows (see table 2,
Pre-Installation Instructions):
a. check for clogged flame jet
can clean if required.
b. Check fuel gas ratios,
make-up gas, and carrier gas
flows.
Test for flame lit: section 8 (FID)
or section 12 (FPD).
See the TCD detector section for
TCD balacing.
Tighten septum nut or replace
septum.
Check for clogged lines or needle,
and for leaks around plunger for
loss of sample.
Replace fuse. Remove ECD PCB
or clean short to ground that
caused fuse to blow.
Set ECD switch S3 to TEST;
select range 10. Disconnect cable
from electrometer input. Check
baseline for 3 setting of S1:
N2HIGH-45mV; N2STD-100mV;
CAP-180mV. If no within -20% to
+60%, replace pcb.
Eliminate metal that contacts
sample, deactivate or change
columns, or derivatize sample.
Presence of water in carrier gas or
excessive injector temperature
stripping of partition liquid. Use a
pure gas source and correct
reaching baseline,
begins to lag.

Injector contaminated.


Thermally
labile
samples.
TCD overloaded.


Injector temperature too
low.


Leaking gas sample
valve.
Improper injections.


Column
incorrectly.


Carrier flow changes.


Injector leak.


Unexpected changes in
column
oven
temperature.



Column contamination.
Colum overload.




3 changes in retention
time.
Changes in retention
time can only be cause
by changes in flow rate
or column temp. Also,
retention time changes
can be accompanied by
sensitivity
changes.
Since retention time
changes can not be
caused by the detector,
the most like source of
the
problem
is
associated with column,
its temp. and flow rate.
installed
27


temperature settings.
Clean or replace insert. May have
to replace column or break off
front portion.
Reduce injector and column
temperature.
Reduce sample size if the filament
protection became activated.
Raise temperature to volatize all
of the components within the
sample.
See the installation section for
checking pneumatic leaks.
Inject smaller amounts and do so
quickly, fully penetrating the
septum and into the injector body.
Column should penetrate injector
body to appropriate depth. See
section 14 or appropriate capillary
injector manual.
Carrier flow changes:
a. Pressure regulator, split ration
valve, or flow controller
changed or malfunctioning.
b. Depleted gas supply. Replace
cylinder when pressure drops
below 100 psig.
c. Leak between regulator and
column inlet.
Septum may leak only at time of
injection or all the time. Replace
if it does.
Column oven temperature can
vary because of:
a. verify that the method hasn’t
been changed.
b. Perform Automatic Tests,
para. 1.2, to test temperature
controller.
c. Insufficient equilibration time
after cool down if ambient
temperature has increased.
d. Column temperature too near
ambient temperature.
e. Exhausted coolant supply or
clogged nozzle or feedline.
Clean or replace column.
Overloaded packed column, will
yield longer retention time:

Loss of liquid phase or
phase degradation.


Poor injection technique


Main power faulty.


Inject switch faulty.


Chart recorder or plotter
faulty.
Chart recorder or plotter
erratic.


4 Increased noise level
(with negligible drift)
a. Expected result
(1×10-12)


Recorder cables faulty.

b. Observed result

AC main power faulty.


Power
inadequate.
ground


Detector assembly and
related hardware faulty.


TSD bead faulty.

28
overloaded absorption columns
yield shorter retention times.
Excessive
temperatures
for
extended periods degrade column.
Replace column.
Excessive slow injection causes
peak distortion.
Erratic or excessively noisy main
power can disrupt normal
operation. See the installation
section.
Faulty inject switch would keep a
temperature
program
at
isothermal. Check switch and
connector. See para.8.8 and table
11 (misc. troubleshooting section)
Erratic paper feed. Check paper
feed against a fixed time.
Exchange with a know good unit,
if possible. See para. 8.6,
Printer/Plotter.
Check cables and connectors,
including shields. Exchange with
good ones.
Check lines for new sources of
interference, such as welders,
ultrasonic cleaners.
Check for faulty grounds,
especially on strip type bench
outlets.
Examine and repair faulty parts:
(see appropriate detector section)
a. Tighten detector tower to base
bolts. Seal must be in good
condition.
b. Signal cable and polarization
BNC cables detented and
fully seated at both ends
c. Replace ferrule at base of
flame trip.
d. Clean deposit from flame tip.
e. Operate detector above 120℃
to eliminate formation of
water condensation.
f. Excessive air drafts over
detectors.
g. Excessive
ambient
air
pollution or high dust level.
Relocate or replace TSD bead.

FPD
faulty.
photomultiplier


ECD source starting to
be inactive.
ECD O2 contamination.
Column
conditions
inferior.


Gas supplier faulty (fuel
gas, make-up, or carrier
gas)



Bad signal cable.
Excessive vibration.



Detector
faulty.



electronics
29


Also, reaccess bead current and
bias value.
Replace
faulty
FPD
photomultiplier tube. Light leaks
can also be a noise source.
ECD
foil
depleted
or
contaminated and needs replacing.
Eliminate leaks and/or scrub gases
Check and column faults:
a. loose or missing plug at
detector end of column will
allow packing material into
the detector.
b. Excessive
bleed
or
contaminated
column.
Recondition
column
or
replace.
Troubleshoot pneumatic system
and repair faults:
a. Check for leaks or interrupted
service.
b. Check tank or supply pressure
regulator. Do not locate in
thermally unstable air, such as
in the direct path of an air
conditioner.
c. Replace any contaminated
supply with clean, dry gas.
d. Replace clogged or spent
filters
e. Leak in gas line. Refer to the
Installation section for leak
testing procedures.
Replace cable.
Isolate instrument from excessive
vibration.
Evaluate the electrometer and
controllers:
a. Cap input of FID, TSD, FPD:
no noise means electrometer
okay. FID: then turn off the
flame with cables connected.
Quiet
baseline
means
polarizer okay. If still noisy,
disconnect the ignite cable; if
noise goes away, polarize is
noisy. Replace pcb to fix
polarizer
b. Perform Automatic Tests,
5 Constant baseline
drift in one direction

TCD baseline dhifts
after each injection.


Continuous oxidation of
TCD filaments.


System contamination
after start up,
particularly with new or
under-conditioned
column.
Detector contamination,
particularly ECD.


Check purity of carrier gas. Try
open tube from injector to
detector. Condition overnight at
elevated termperature.


TCD flow not balanced.
Detector temperature
unstable.



Ionization detector
leaking at seal.


FID flame tip ferrule
cracked.


Thermal
example 1.

Check flow rates of both channels.
Allow sufficient time for detector
stabilization
after
changing
detector temperature.
Replace aluminum washer. See
appropriate detector section for
procedure.
Replace flame tip ferrule. See
appropriate detector section for
procedure.
Check temperature stability of
injector, column. And detector,
and/or systematically change the

6 cycling baseline.
para.
1.2.
Intermittent
problems may be difficult to
locate. If possible, these are
best isolated by an exchange
of known good component.
c. Check time constant switch.
Use the longest possible
setting.
d. Dirty or contaminated cable
or pcb connectors. Carefully
clean with a pencil eraser or
similar abrasive. Cycling the
connector open and closed
several
times
may
be
sufficient cleaning.
Faulty filament temperature limit
circuit. Unplug bridge from TCD
PCB; If (a.) background fault
43/53 is not displayed, replace
pcb, or (b.) switch set to 490 with
oxidizing sample; use 390, if
possible.
Perform pressure leak check; TCD
cell may need to be replaced (O2
back diffusion at any fitting could
be responsible).
Overnight bakeout procedure at
the
recommended
upper
conditioning temperatures for the
column.
problem,
30
7 Spikes at irregular
intervals.

Pneumatic problem in
pressure
regulator,
example 2.


Pneumatic problem in
flow controller, example
3.
Detector contamination.




Electrical interference
from external source.


Quick
atmospheric
pressure changes from
opening and closing
doors, blowers, etc.
FID
only—Dust
particles
or
other
foreign material burned
inflame.
Column fittings loose.
Electronic
circuitry
defective.

Connection
between
detector and electronics
poor.
TSD, FID, OR ECD





31
setpoint by 10℃ of each or the
zones in sequence. The pattern ill
change within one F period after
the associated controller is
changed.
a. Change the setpoint pressure of
the pressure regulator to a high
pressure (+5 psig); if cycling is
interrupted, then resumes after
approximately 30 seconds, the
regulator is not controlling at this
pressure. Try increasing pressure,
replacing regulator. Eliminate air
current
across
pneumatic
compartment and tank regulator.
b. Low gas pressure from source.
Replace flow controller.
Check
decontamination
procedures for FID tip. Some
spiking is common when the
flame has been off for over a day.
Air borne particulates are most
frequently the cause; therefore,
protecting the exit orifice from
atmospheric particles falling into
the detector is important.
Check for heavy current drains on
the same power circuit as the
chromatograph. An isolation
transformer may be needed.
Relocate instrument to minimize
the problem. Do not locate under
heater or air conditioner blowers.

Keep detector free of dust
particles. Blow out or vacuum
detector to remove dust.



Tighten column connections.
Perform Automatic Tests per para.
1.1. Cap input of FID, TSD, FPD:
no electronic fault if spikes go
away.
Check for faulty detector cables.

Clean detector probe insulators
8 short spikes or peaks
at regular intervals.




9 Rising baseline when
temperature
programming

only—Dirty
detector
probe insulators.
Condensation in flow
lines causing carrier gas
to bubble through.
FID only — water
condensation
in
hydrogen line coming
from
hydrogen
generator
TCD only — bubble
flowmeter with high
liquid level attached to
detector exit line.
TCD only — carrier gas
moisture
filters
exhausted.
Increase in column or
septum bleed when
temperature rises.


Remove water from line and
replace or regenerate filter.

Remove flowmeter tube from exit
line.

Replace or regenerate carrier gas
filters.

Recondition column and septum;
use high-temperature septum. Use
column with different phase,
cross-linked phase, or smaller film
thickness.
Adjust reference column flow rate
at maximum temperature to zero
baseline.
Make sure instrument connected
to good earth ground.
Recalibrate according to chart
recorder manual.

Column flow rates not
balanced

10 Baseline stepping.
Baseline does not return
to zero, attenuation is
incorrect, peaks are
flat-topped.

Instrument not properly
grounded.
Excessive
recorder
deadband.

11 Baseline can not be
zeroed.

Detector
control
circuitry defective.
Autozero not enabled.

Excessive background
from column bleed.
FID or ECD only —
detector contaminated.
ECD only – O2
contamination.
TCD only – detector
filaments out of balance
FID, TSD only – FID /
TSD selector switch on








32
with clean solvent. Do not touch
clean insulators with fingers.
Heat lines to remove condensation
while purging with dry gas.






Perform Automatic Tests per
para.1.2.
Be sure Autozero enabled in
method. Autozero functions only
when GC is READY.
Condition column; use different
column.
Clean detector base and detector
(see appropriate detector section)
Eliminate leaks or scrub gas.
Check
filament
resistances.
Replace detector if necessary.
Set FID/TSD selector swith to
appropriate position.
12 Unexpected peaks
13 Unresolved peaks

electrometer set to
wrong position.
High boiling sample
components
eluting
from previous injection.


Ghost
peaks
from
septum, unconditioned
column, contaminated
carrier gas or previous
sample components.


Column
oven
temperature too high.
Column too short.
Mobile phase has baked
off of column support
material.
Incorrect column for
application.
Carrier gas flow rate too
high.
Poor injection technique

Perform
rapid
temperature
program after each analysis to
purge high impurities out of
column.
To confirm ghost peaks from
septum or contamination upstream
from column, do the following:
a. Measure cooldown and
re-equilibration time.
b. Run temperature program and
measure the magnitude of ghost
peak.
c. Allow twice the cool down and
equilibration time.
d. Repeat program and peak
measurement. If peaks are
doubled, this confirms upstream
contamination.
To
isolate
contamination
from
other
upstream causes, install the
TeflonR injector blank-offplug and
repeat above procedure. If ghost
peaks disappear, the septum is at
fault and can be minimized by: (1)
Better quality septum (2) Lower
injector
temperature(3)
Preconditioning
septum(4)Very
short
initial
hold
before
programming.
If ghost peaks persist, upstream
contamination is indicated and can
be minimized by installing a 1/8”
× 6” long molecular sieve trap
between the flow controller and
the injector.
Reduce column oven temperature


Use longer column.
Replace or repack column

Consult your nearest service
center for column advice.
Reduce carrier gas flow rate.





33


Review
sample
techniques.
injection

Printer/plotter
or
recorder attenuation too
low.


Electronic or detector
saturation due to sample
overload.

15 Round-topped peaks

Detector overload

16 Negative peaks

TCD only: sample
injected into wrong
column.
Contamination
in
carrier gas (some peaks
reversed in direction of
response).

Inject sample into correct column.



Recorder leads reversed
Contamination
in
detector.



Partial
response.

Reverse output singal.
Vacancy
chromatography.
Particularly in trace analysis,
concentration of a certain
component it greater in the carrier
gas (impurity) than it is in the
sample. Condition is common
with TCD when leak into carrier
gas. Contaminated septa, depleted
gas filters, etc., can contribute to
this condition. Successful trace
analysis demands that the system
be at optimum performance:
a. Be sure all gas inlets filters are
functional that the highest purity
is assured.
b. Use cylinder gas of proven
purity.
c. System may need to be leak
checked.
Check recorder connection.
Decontaminate
or
replace
insulators in FID or TSD.
Decontaminate ECD foil and
insulators.
“W” shaped peaks: when using a
TCD to analyze for H2; with H2
concentration above 3%, negative
H2 peak results, with H2
concentration below 3%, positive
H2 peak results. Phenomenon can
be minimized by changing the
14 Square-topped peaks

negative
34
An off scale solvent peak ill result
in this shape – not a defect. Data
system not involved unless
“overrange” message.
Select less sensitive range setting.
The best range setting is the most
sensitive position that will hold all
peaks of interest on scale at an
attenuation of 1024 with a 1 mV
chart recorder. Reduce sample
volume or concertration.
Reduce sample volume or
concentration.
17 Extra peaks
Heavy residual material
eluting from previous
sample injection(1)。
Condesed moisture and
other impurities from
carrier
gas
eluting
during temperature –
programmed run (1)
Air peak (2)(TCD only)


Desorption
from
column packing when
solvent is injected (2)


Sample
decomposition(2,3)


Contamination
sample(3)


Sample interaction with
mobile phase or solid
support of column
packing(1,2,3)
Contamination
from
glassware,
syringes,
etc.(1,2,3)
Sample size too large.




18. FID only—Sudden
drop off of otherwise
normal
peak.
Pen
returns to level below
previous baseline. FID
flame
becomes
extinguished.






19. Negative dips after
peaks


sample loop size or by using 8%
H2, 92% He carrier gas, which
always yields negative H2 peaks.
Allow sufficient time for previous
sample compounds to elute.

Install, replace
carrier gas filter.

Normal when making signal
syringe injections.
Make several solvent injections
and recondition column.
or
regenerate
Reduce injector temperature. Use
different column if packing
material is causing or catalyzing
decomposition.
Test sample contamination. Use
fresh test sample.

Use different column. Consult the
local dealer.

Make sure glassware, syringes,
ect. ,are clean.

Carrier gas flow rate too
high.
Flame tip orifice too
small.
Flame tip plugged.
Loss of hydrogen or air.

Reduce sample size. Reignite
flame.
Adjust carrier gas flow rate.

Replace flame tip.


Sample contains more
oxygen
than
combustion air, causing
flashback.
Contaminated detector,
ECD.
High column bleed.

Clean or replace flame tip.
Check hydrogen and air supplies.
Re-establish proper flow rates.
Dilute sample with inert gas, or
use oxygen rather than air to
support combustion.
35


Clean detector as described in the
ECD detector section.
Condition column.
20. Flash
only—Loss
resolution.
injection
of

Flash
injector
not
seating in injector body.


Glass wool in injector
insert is missing or
positioned too high in
insert.
Sample injected too
rapidly.
Temperature of the
column too high.


21.Insufficient
resolution
(Between
peak1 and peak2)


Non-optimum flows.

Column too short.

Poor choice of column
liquid phase.
36

Clean inside of injector body and
reinstall glass insert. If using a
1/8〞 column with 1/4〞injector,
be sure column dose not extend
too far into injector body.
Place glass wool loosely in lower
third of insert.
Decrease rate of injection to 10μl
or less per second.
 Repeat the analysis with a lower
column temperature by about
25 ℃ . Depending on the
relationship to the other peaks, a
temperature program might be
advantageous.
 The column flow rate usually will
cause only small differences in
resolution unless a very low flow
is used. Suggested flows for best
resolution are shown below:
Column Internal Diameter
2mm
4mm
N2
12-15 ml/min 25-30 ml/min
He
24-30 ml/min 50-60 ml/min
 Although the number of plates
increases linearly with an increase
in column length (providing the
packing efficiency can be
maintained), the resolution only
increase by the square root of the
increase in column length, i.e.,a4
times longer column will only
increase resolution by 2 fold.
Another
consideration
to
increasing column length is the
resulting increase in column inlet
pressure. Above 40 psig, leak
problems start to be a limiting
factor. Usually a 1-3 meter, 2mm
ID
packed
column
is
recommended
for
maximum
column length.
 Selecting a liquid phase of
different polarity may help if there
is a difference in polarity of the
components.
If 1 is more polar than 2:
a.


22. Leading peaks
Those peaks exhibiting
a slow rise to apex with
a sharp drop to baseline.




Detector time constant
too long.
Column degradation.
Column overload due to
an excessive amount of
sample.
Column overload due to
low
column
temperature.
Column overload due to
insufficient liquid phase
on the solid support,
e.g., possibly from
extended operation at
excessive temperature,
or H2 in carrier gas, H2O
in carrier gas.
Carrier velocity too low.
23. Reverse leading
peaks
Those peaks exhibiting
a sharp rise to apex
followed by a slow
constant
return
to
baseline.



Column overload due to
an excessive amount of
sample.
Column temperature too
high.
Column velocity too
low.
37


A lower polarity liquid phase
will improve the separation.
b. A slightly higher polarity
liquid phase will reduce
separation or cause co-elution
c. A much higher polarity liquid
phase may provide separation,
with 2 eluting before 1.
If 1 is less than 2:
a. A lower polarity liquid phase
will reduce separation or
might yield a separation with
2 eluting before 1.
b. A more polar liquid phase
will yield more separation.
Use FAST switch position for
very narrow capillary peaks.
Replace or repack column.

Inject a small volume of sample or
dilute the sample mixture.

Increase the column temperature.

1. Replace the column with a
new one.
2. Change column composition
to a higher ﹪liquid phase coating
(about 20% is maximum possible
loading for Chrom W, 10% for
Chrom G, and 40% for Chrom P)
 Increase column flow rate.
CONDITIONS: Adsorption column
operating at a temperature above the
boiling point of the compound (the
usual case) or partition column
operating at a temperature above the
boiling point at the compound, such as
a solvent (the usual case).
 Reduce the volume of sample
injected.

Reduce column temperature

Increase column flow rate.
9 ELECTRONIC HARDWARE LOCATIONS
Refer to the figures on these pages for electronic hardware locations.
ELECTRONIC HARDWARE LOCATIONS
38
39
40
HELP HOW TO USE THIS MANUAL
When there is a question about the current display on the instrument, press [SHIFT] then hold
[HELP] to display a number reference in this manual where additional information about the
current display is located.
Turn to that number in this manual, locate the display in question, and read the information
explaining use of the parameter or more information about display contents. Upon release of
[HELP], the display in question returns. To find any individual display, use the index at the back
of this manual.
★★★★★★★★★★★★★★★★★★★
If you are not yet familiar with the operation of your instrument, turn first to the Operation
section for key functions, operation, and method building exercises.
41
OPERATIONS Keys
[BUILD/MODIFY ]
1. Display: SELECT METHOD / SECTION OR TABLE
The Build/Modify light is on. Active or inactive methods and tables can be built and modified.
Press:- one of the four Method keys (not digits on the number pad), or
- a SECTION CONTROL key, such as [COLUMN], or
- [GC CONFIGURE], or
- [RACK TABLE] or [SEQUENCE TABLE] automation keys.
[SHIFT] [ACTIVE LINE]
2. Display: SELECT SECTION OR TABLE
Active line provides quick access to display and modify the controlling program (or initial
conditions) within an active method section or automation table.
The BUILD/MODIFY and active method lights are ON. The GC or automation must be running.
Note that the GC is operating and therefore control can pass to the next program. The displayed
program is not updated since the instrument is in Build/Modify mode.
Other possible displays are:
DETECTOR A OR B?
SECTION NOT IN METHOD
ILLEGAL SECTION
TABLE NOT ACTIVE
INJ A-INJ B OR AUX?
AUXILIARY (INJ B) SECTION? NO
INJECTOR A/B OFF IN GC CONFIGURE
AUXIL IARY OFF IN GC CONFIGURE
DETECTOR A/B OFF IN GC CONFIGURE
NOT IN RUN
NOT IN AUTOMATION
Auxiliary is part of the INJECTOR method section. See HELP 13. For a description of
ILLEGAL SECTION, see HELP 35.
[ACTIVATE]
3. Display: SELECT METHOD OR TABLE
The activate operation is used to change which method controls the GC, i.e., the active method.
Press the desired method number key. The light beside the method key comes on, the GC.
immediately assumes the conditions of the new method, and the display informs:
METHOD ACTIVATED or TABLE ACTIVATED
[ACTIVATE]
ACTIVATE is also used to set up for automation. Build the desired Rack or Sequence automation
42
table, activate the table, and begin automation by pressing [START].
Press [STATUS] at any time to find out which method and table is currently active. The light by
the active method number key and table key will be ON.
Under certain conditions the activate operation is denied:
METHOD RUNNING
AUTOMATION ACTIVE
TABLE ACTIVE
TABLE NOT BUILT
UNDER REMOTE CONTROL
[COPY]
4. Display: SELECT METHOD TO COPY FROM
The copy action is used to copy one entire method over another.
METHODS keys.
Press one of the four
Display: SELECT METHOD TO COPY TO
Press the METHODS key for the method that is to be the copy. Note: The original contents of the
copy will be lost. Copies to locked or running methods are not allowed.
Display to:
COPY COMPLETE
METHOD LOCKED
METHOD RUNNING
[SHIFT] [LOCK/UNLOCK]
5. Display: ENTER LOCK CODE
[][][][]
Locked methods and tables cannot be modified. Any or all can be locked, one at a time. The user
lock code is first required. Enter the same lock code that was entered in the GC Configure table.
If it was never changed in the GC Configure table, the lock code is 0 (zero). Incorrect codes will
not be accepted.
Display: SELECT METHOD OR TABLE
After entry of the correct lock code, press the desired method number key, [RACK TABLE],
[SEQUENCE TABLE], or [GC CONFIGURE].
[SHIFT] [LOCK/UNLOCK] (cont.)
Display to: METHOD LOCKED (OR UNLOCKED)
TABLE LOCKED (OR UNLOCKED)
or
If the selected method or table was not locked, this action locked it. If it was locked, it becomes
unlocked.
43
[DELETE PROGRAM]
6. Display: SELECT METHOD/SECTION OR TABLE
DELETE PROGRAM is used to delete individual programs that have been built into method
sections or automation tables. Only entries labeled PRGM are deleted, one at a time, by
requesting the specific program by number.
Press the method, method section, or table key containing the program to delete. Follow the
displays where applicable:
SELECT SECTION
DETECTOR A OR B?
AUXILIARY (INJECTOR B) SECTION? NO
INJ A-INJ B OR AUX?
Display:
ENTER PROGRAM NO. TO DELETE---
Enter the desired program number to be deleted.
Display:
PROGRAM (OR TABLE) DELETED
Remaining programs are renumbered if necessary. Upon deletion of the last line of either the
Rack or Sequence tables, the entire table is deleted.
Sometimes deletion is not performed:
METHOD RUNNING
METHOD (or TABLE) LOCKED
PROGRAM NOT IN METHOD
PROGRAMS NOT BUILT
PROGRAM NOT IN TA8LE
TABLE NOT BUILT
SECTION NOT IN METHOD
TABLE ACTIVE
If the method section is illegal, see HELP 35.
[DELETE SECTION/TABLE)
7. Display: SELECT METHOD / SECTION OR TABLE
Specified method sections or entire automation tables can be deleted. Entire methods cannot be
deleted at once. The GC Configure table cannot be deleted.
Method sections are always deleted from the method whose light is on.
Display: SELECT SECTION TO DELETE
Press the key for the Method section to delete.
44
Display to: SECTION (or TABLE) DELETED or
SECTION CLEARED
or
INJ A CLEARED/INJ B DELETED or
DET OVEN A OR B OR DET A OR B?
Required method sections (COLUMN, INJECTOR, and DETECTOR) sections are not deleted,
but are cleared (returned to the preset values of an unmodified method section with no
programs).
Display:
DELETE DETECTOR A OR B?
The instrument has two detectors. After one detector section is deleted, the other can only be
cleared.
Display:
DELETE AUX (INJECTOR B) SECTION ONLY? YES or
INJ A-INJ B OR AUX?
Optional auxiliary zone or INJECTOR B control is part of the INJECTOR method section. Press
[Yes] [ENTER] to delete just the auxiliary/INJECTOR B part or [No] [ENTER] to delete the
Auxiliary or INJECTOR B section and clear the INJECTOR section to presets.
Under certain conditions deletion cannot be performed:
SECTION NOT IN METHOD
METHOD (OR TABLE) LOCKED.
[TUNE ON]
8. Display:
METHOD RUNNING
TABLE NOT BUILT
SELECT SECTION TO TUNE
Press [DETECTOR], or [PLOTTER], or [RELAY] and [ENTER].
TUNE is specified as the program time during method building of these sections. That program
can then be executed under user control during the method run using Tune mode. The current run
time is inserted into the method.
See The Special Uses of Keys section for use of Tune mode. Temperature programs cannot be
tuned.
[TUNE ON] (cont.)
Press [TUNE ON] to enter Tune mode. The instrument remains in Tune mode until another
OPERATIONS key is pressed. When a tunable method section is selected, the first tunable
program is displayed:
TUNE FID A/B PRGM n
TUNE PLOTTER PRGM n
TUNE RELAY PRGM n
45
DISPLAY CONTROL keys [▲] and [▼] can be used to display the programs in that section that
have TUNE entered for the program time. Tune mode can be entered at any time. but to actually
tune a program. The GC must be in RUN.
Display the chosen program and press [ENTER] at the appropriate time to execute the program
and replace TUNE in the method with the current run time. The program will then be inserted in
the proper sequence, according to the time of the tune event, and programs Will be renumbered.
Tunable programs are not renumbered until they are tuned. Other programs in the method are
executed at their program times.
When a program is tuned, the next TUNE program in the section will be displayed. The user can
select another section to tune at any point in tune mode.
Display:
DETECTOR A OR B?
Detector A and B sections are tuned independently. To tune the other detector section, press
[TUNE ON] and [DETECTOR] again.
Display:
NO MORE TUNE ENTRIES
All programs in that section have been tuned. Select another method section to tune, or press an
OPERATIONS key to exit tune mode.
Under certain circumstances, tuning is not allowed. The display informs and the instrument exits
Tune mode:
NOT IN RUN
METHOD LOCKED
ILLEGAL SECTION
If the method section is illegal, see HELP 35.
[PRINT] and [SHIFT] [REPORT]
9. Display: SELECT METHOD /SECTION OR TABLE
Press one of the four METHODS keys, [GC CONFIGURE], [RACK TABLE] or [SEQUENCE
TABLE] to print the current entries. If a method section key is chosen, that section will be
printed for the method whose light is ON (currently displayed method).
Display:
SELECT SECTION OR ENTER FOR ALL
A method key was selected for printing. Press [ENTER] to print the entire method, or a
SECTION CONTROL key for a single section.
Display: PRINTING - SELECT NEXT OPERATION
46
Printing is in progress. This display remains until another OPERATIONS key is pressed.
Displays: PLOTTER BUSY and PAPER OUT
The print or plot operation is not executed. The print request must be repeated when the plotter is
free or after paper is loaded. Refer to the Quick Reference Manual for paper loading procedure.
[SHIFT] [REPORT]
Press [PRINT] [SHIFT] [REPORT] to print the report of the most recent GC run. To print the run
log as well, enable it in that method, even if the run is over. The information is retained until the
GC goes to RUN again.
Diagnostic faults that are detected are added to the report when they occur, even if the instrument
is not in RUN. Thus diagnostic results can be printed at any time by pressing [PRINT] [SHIFT]
[REPORT]. If the total report is not wanted, press [(PLOTTER) STOP] as soon as the
diagnostics portion is printed. Pressing [RESET] clears the diagnostics messages from the report.
10. [STATUS]
Status displays include overall GC Status, actual temperatures, NOT READY conditions, fault
messages, and elapsed run time clock. The STATUS light and active methods, light are ON.
Entries cannot be made into any status display. All STATUS displays have the prompt:
INSTR STATUS - NO USER ENTRY
Pressing [ENTER] advances the display to additional Status messages.
CONTROL or AUTOMATION
CONTROL keys for individual status.
Press SECTION
[STATUS] (cont.)
Display:
COL aaao INJ aaao DET aaao
Display:
COL aaao INJ aaao AUX aaao DET aaao
Display:
INJ B aaao DET B aaao AUX aaao
Display:
PRES A/B aaa VEL A/B aa SPLITRA A/B aaa
Current actual temperatures for column, injector, auxiliary and detector ovens are displayed.
(Auxiliary is part of the INJECTOR section and must be enabled in GC Configure). If an actual
temperature differs from its set point by more than a tolerated amount, the degree symbol by that
zone blinks. To view set points as well, press a SECTION CONTROL key.
Display: METHOD x RUN xxxxx END xxxxx MIN
Display shows the currently active method number, the current run time, and the method end
time.
Display: METHOD x COMPUTING
47
Display indicates that the method is processing peaks in preparation for printing a report. While
in this state, you may modify this or another method, examine status, or change instrument set
points without interfering with the computation. The printer/plotter will not print or plot while in
the COMPUTING state. The display will be PLOTTER BUSY.
Display: METHOD x STABILIZE xxxxx MIN
When all thermal zones have reached their set point values, the instrument advances from NOT
READY to STABILIZE, the NOT READY light remains ON, and the THERMAL STABILIZE
TIME entered in the GC Configure table counts down to GC READY.
Certain conditions, for example detector baseline out of the Auto zero range, prevent the
instrument from advancing to READY. The displayed stabilize time will show 0.00. Press
[RESET] and the GC will go to READY, or turn detector initial auto zero off in the method.
Display: METHOD x MONITOR
MONITOR state exists between the end of STABILIZATION and READY, during which time
the baseline noise is evaluated. At the end of MONITOR, the noise value is printed and the
method goes READY. Baseline noise is MONITORed each time a method is manually activated
or activated by automation.
[STATUS] (cont.)
Display: METHOD x MONITOR
(cont.)
Since the baseline noise value is used to set, the sensitivity of the peak detection logic, it is
important not to introduce extraneous noise during the MONITOR state, such as by pressing
RESET, changing detector parameters, opening the column oven, and making sure detectors have
stabilized before putting the GC in the MONITOR state. If an erroneous noise value is detected,
a new noise monitor can be achieved by reactivating the same method, after removing the cause
of the original error.
Display: METHOD x A/S WAITING xxxxx MIN
The active method contains the AUTOSAMPLER section and is waiting to start the next
sampling cycle. If the method AUTOSAMPLER section specifies a time for START A/S AFTER
RUN START. Then this waiting time countdown is displayed between injections.
Display: METHOD x A/S SAMPLING
The Autosampler is in the sampling cycle.
Display: METHOD x WAITING FOR EXT DEVEICE
The GC is NOT READY because it is waiting for a ready signal from an external device. This
48
wait is enabled in the GC Configure table.
Display: METHOD x INACTIVE-END TIME xxxxx
Pressing an inactive METHODS key while the STATUS light is ON displays the inactive status
plus method end time.
Display:
FAULT XX
The STATUS light is blinking to indicate that fault(s) have been detected by the instrument
background diagnostics. Refer to the Diagnostics/Troubleshooting Section for a complete
listing of fault messages and assistance.
Display: ILLEGAL METHOD x
The active method contains an illegal method section, caused by changing the instrument
configuration after the method section was built, i.e., std. injector removed and OCI injector
installed. The STATUS light blinks and the GC is held NOT READY. Press [STATUS]
[ENTER] to display the fault(s). Refer to the Diagnostics section for a listing of fault messages.
NOTE: Correct an illegal method by correcting the hardware configuration or deleting the
illegal method section.
COLUMN Method Section
([BUILD/MODIFY]) [COLUMN]
11. Display: INITIAL COLUMN TEMP
Enter the isothermal column oven temperature, or the initial temperature for temperature
programming. Temperatures are in degrees C. The entry range given in the prompt is from -99o
to the COLUMN TEMP LIMIT entered in the GC Configure table or 1o less than the next
temperature if a temperature program follows.
Temperatures can be changed while the method is running, but oven will not cool down below
the current actual value until the last column temp. Hold time has expired.
If COLUMN STANDBY TEMP is enabled in the GC Configure table, the active method
temperature set point will not be effective and the GC will be held NOT READY until
[RESET] is pressed or STANDBY TEMP is disabled.
If optional cryogenic control is to be used, column coolant must be enabled in the GC Configure
table.
Display:
INITIAL COL HOLD TIME
Enter the time that the initial column temp is to be held before the first temp program starts in the
run. For isothermal operation, this determines the End Time of the GC Run Unless other method
sections have time or temp. Programs which take a longer time to complete.
The range of time entries allowed is from 0 to 650 minutes for isothermal operation. If column
49
temperature programs follow, the maximum hold time is reduced to 650 minutes minus the time
required for the programs. These values are calculated and displayed when [PROMPT] is pressed.
INF is infinite time, and will keep the GC in RUN until [RESET] is pressed.
Display:
TEMP PROGRAM COLUMN? NO
If column temp programming is required, enter [YES]. A total of 4 column temperature programs
can be built.
NOTE: No programs can be added after an infinite hold time, and any which are already
present will be deleted. If a temperature entry is equal to the COLUMN TEMP LIMIT,
then no further programs may be added.
NOTE: No value is displayed in the entry field when adding new programs and the
program is considered incomplete until a legal value is entered. When modifying a
program already built, the previously entered value is displayed.
[COLUMN]
(cont.)
Display: PRGM 1 FINAL COL TEMP
Allowable entries range from lo above the INITIAL COLUMN TEMP (or previous program
temperature) to lo less than the final temperature of a following program, or, if this in the last
program built, the COLUMN TEMP LIMIT. Press [PROMPT] to display these calculated values.
Display:
PRGM 1 COL RATE IN o/MIN
Enter desired programming rate, from 0.1 to 50.0oC per minute, or the lowest rate which fits into
the remaining run time. Press [PROMPT] to display legal values.
Display:
PRGM 1 COL HOLD TIME
Enter the time that the final temperature of this same program number is to be held until the next
program begins. If this is the last program, the column oven cools down at the end of this final
hold time.
Display: FLOW A/B IN ML/MIN
Only On instruments With electronic flow readout. Enter desired column flow rate, and adjust
flow controller at the pneumatics panel While observing flow status.
Display:
PRESSURE A/8 IN PSIG
Displayed only on GC's equipped with electronic pressure readout for column A or B. Enter
desired column head pressure in psi, and adjust pressure regulator at the pneumatics panel while
observing pressure status.
50
Display: SPLIT RATIO A/B x TO
Displayed only for capillary systems with electronic flow readout. Enter the desired split ratio
xx:l as "xx". Adjust the splitter flow while observing split ratio status until the correct split ratio
is achieved.
Display:
COLUMN IN CM/SEC VELOCITY A/B
Enter the average linear velocity for the carrier gas in use, and then adjust the gas flow rate while
observing status. Optimum velocity for best separation efficiency can be determined from Van
Demeter plots. Refer to the Columns section for setting velocity.
([STATUS]) [COLUMN]
12. Displays: COL aaao SET sss or OFF
COL aaao SET sss STABILIZE
COL aaao SET sss RATE rr.r
.
o
COL aaa SET sss HOLD xxx.xx
COL aaao SET sss EQUILIBRATING
COL aaao SET sss STANDBY
The actual column oven temperature in oC is displayed, followed by its current set point in the
active method. The right-hand field updates to give additional current information:
- The GC is in the STABILIZE state.
- RATE and rate set point are displayed during a temp. program. The displayed temperatures are
updated according to the rate.
- HOLD times are displayed counting down.
- EQUILIBRATING indicates that the temp program(s) are completed while the GC is still in
run.
- STANDBY flags that the set point temperature is the COLUMN STANDBY TEMP set in the
Configure table (see HELP 29), and the GC stays NOT READY. Return control to the active
method by pressing [RESET].
If the actual temperature is out of tolerance of its set point value, the degree symbol flashes, the
NOT READY light comes on, and a column temperature error message is generated if the GC is
in RUN.
Display:
FLOW A/B aaa SET xxx
Requires optional flow readout module. Actual and set point flow for column A or B is displayed
in ml/ minute. If WAIT FOR FLOW READY? is YES in the GC Configure table, and the actual
flow drops to half of the set point value, the actual value blinks, and the GC goes NOT READY.
Display:
PRESSURE A/B aaa SET 10
Optional pressure readout module must be present. The pressure for column A or B is displayed
in psig. If WAIT FOR PRESSURE READY? is YES in the GC Configure table, the GC goes
NOT READY if the actual pressure falls to half the set point value, the display blinks, and the
51
GC goes NOT READY.
Display:
SPLIT RATIO A/B aa SET ss
Actual split ratio aa:l and method set point value ss:l are displayed for col. A or B. If WAIT FOR
SPLIT RATIO READY? Is YES in the GC Configure table, then if the actual value is less than
half the set point, the GC goes NOT READY and the actual value blinks on the display.
([STATUS]) [COLUMN] (cont.)
Display: COL A/B VELO aa CM/SEC ss
Column A or B average linear velocity is displayed for the carrier gas in use (entered in the GC
Configure table). If WAIT FOR VELOCITY READY? is YES in the GC Configure table, then if
the velocity falls to less than half the set point value, the instrument goes NOT READY and the
display blinks.
INJECTOR Method Section
( [BUILD/MODIFY] ) [INJECTOR]
13. Display: INJECTOR A/B OFF IN GC CONFIGURE
Injector, detector, and auxiliary ovens are turned ON or OFF in the GC Configure table. Refer to
HELP 31 for the appropriate INJ, DET, or AUX zone ON/OFF displays.
Display:
INJECTOR A/B TEMP
Injector temperature is part of every method. The allowable entry range is displayed by pressing
[PROMPT]. The maximum temperature that can be entered is the INJECTOR TEMP LIMIT set
in the GC Configure table.
Temperature Programmable Capillary On-Column Injector
If the GC is equipped with the temperature programmable capillary on-column injector, the
injector switch (INJ) on the Temperature Control PC Board must be in the O.C.I. position. This
enables displays and control for temperature programming this injector. Up to two injector
temperature programs can be built.
COOLANT TO INJECTOR? must be enabled in the GC Configure table for correct operation
and rapid cool down of this injector.
NOTE: The control parameters for the STD and O.C.I. injector switch positions are
optimized for the two modes. Use the correct position for the injector type installed to
obtain stable and reliable control.
Displays:
INITIAL INJECTOR A TEMP
INITIAL INJ A HOLD TIME
TEMP PROGRAM INJECTOR A? NO
PRGM l FINAL INJ A TEMP --PRGM l INJ A RATE IN o/MIN --PRGM l HOLD TIME
ADD NEXT INJECTOR PROGRAM? NO
See COLUMN Help (HELP 11) for a description of temperature programming. The column,
injector, and auxiliary zones are handled in the same way.
52
([BUILD/MODIFY]) [INJECTOR]
Display: AUXILIARY OFF IN GC CONFIGURE
Injector, detector, and auxiliary ovens are turned ON or OFF in the GC Configure table. Refer to
HELP 31 for the appropriate INJ, DET, or AUX zone ON/OFF displays.
Display:
ADD AUXILIARY SECTION? NO or ADD INJECTOR B SECTION? NO
The Temperature Control PC Board has a connector which can be used to control the temperature
of an auxiliary zone (such as a sampling valve) or INJECTOR B. Auxiliary control is enabled in
the GC Configure table (TURN HARDWARE ON-OFF?). This adds displays to the injector
section of methods and reports the actual auxiliary zone temperature in STATUS.
Up to two auxiliary temperature programs can be built. Control of an auxiliary cryogenic valve is
not provided.
Displays:
INITIAL AUX (INJ B) TEMP
INITIAL AUX (INJ B) HOLD TIME
TEMP PROGRAM AUXILIARY (INJECTOR B)? NO
PRGM l FINAL AUX (INJ B) TEMP --PRGM l AUX (INJ B) RATE IN o/MIN --PRGM l AUX (INJ B) HOLD TIME
ADD NEXT AUXILIARY (INJECTOR B) PROGRAM? No
See COLUMN HELP (HELP 11) for a description of temperature programming entries and
prompts. The column, injector, and auxiliary zones are handled in the same way.
( [STATUS])
14. Displays:
[INJECTOR]
INJ A/B aaao SET 100 or OFF
INJ A/B aaao SET sss STABILIZE
INJ A/B aaao SET sss RATE rrrr
INJ A/B aaao SET sss HOLD xxx.xx
INJ A/B aaao SET ss (EQUILIBRATING)
The actual injector temperature in oC is displayed followed by its current set point in the active
method.
The right-hand field updates to give additional current information:
- The GC is in the STABILIZE state,
- RATE and rate set point are displayed during a temperature program.
The displayed temperatures are updated according to the rate.
- HOLD times are displayed counting down.
- EQUILIBRATING indicates that the temperature program(s) are completed while the GC
is still in run.
( [STATUS]) [INJECTOR] (cont.)
If the actual temperature is out of tolerance of its setpoint value, the degree sign flashes, the Not
Ready light comes on, and an 'Inj Temp' error message is generated if the GC is in Run.
53
Displays:
AUX (INJ B) aaao SET sss or OFF
AUX (INJ B) aaao SET sss STABILIZE
AUX (INJ B) aaao SET sss RATE rrrr
AUX (INJ B) aaao SET sss HOLD xxx.xx
AUX {INJ B) aaao SET ss (EQUILIBRATING)
Status for the auxiliary temperature controlled zone is displayed by pressing [Status] + [Injector]
+ [Enter]. Auxiliary control must first be enabled in GC Configure. Display descriptions are the
same as above for Injector.
DETECTOR Method Section
15. ([BUILD/MODIFY]) [DETECTOR]
DETECTOR HELP: A variety of detector types are available and can be mounted in
position A or B. This information is included in each detector display. Help information for
each detector type is treated separately in the following pages. Descriptions include
detector method building (HELP 15) and detector status (HELP 16).
Display:
DET OVEN A/B OFF IN GC CONFIGURE
Injector, detector, and auxiliary ovens are turned ON or OFF in the GC Configure table. Refer to
HELP 31 for the appropriate INJ, DET, or AUX zone ON/OFF displays.
Display:
DETECTOR A/B TEMP
Enter the desired detector temperature in oC. There is a single detector thermal zone for one or
more detectors. The detector temperature should be set higher than that of the column oven to
prevent condensation in the detector towers. Temperatures higher than the DETECTOR TEMP
LIMIT set in the GC Configure table cannot be entered, as reflected by the Prompt, unless the
GC Configure table value is first changed.
DUAL DETECTOR INSTRUMENTS:
Display: DETECTOR A/B OFF IN GC CONFIGURE
Injector, detector, and auxiliary ovens are turned ON or OFF in the GC Configure table. Refer to
HELP 31 for the appropriate INJ, DET, or AUX zone ON/OFF displays.
Display: DETECTOR A OR B? A
Choose the detector A or B to be edited.
([BUILD/ MODIFY]) [DETECTOR] (cont.)
Display:
ADD DET B SECTION?
YES adds method control of the indicated detector, type, and location A or B. The method must
contain at least one DETECTOR section.
Display:
SIGNAL A OR B TO DATA SYSTEM
54
This display is part of the Detector A method section in dual detector instruments, and is seen
only if the Serial I/O option is present, and if SIGNAL SWITCHING TO DATA SYSTEM is
enabled in the GC Configure table. (See the External Data Systems section for Serial I/O cabling
connections.) Enter the desired detector, A or B, to be selected for the single control station line
to the data system. Signal selection is time programmable in the DETECTOR A method section.
Display:
PRGM l SIGNAL TO DATA SYSTEM
Detector A method section can time program signal switching. Thus both detectors can be
monitored during the run on a single chromatogram by switching between the detector A and B
signals during the run.
To build detector time programs, refer to the displays that correspond to the detector type
installed.
([BUILD/MODIFY]) [DETECTOR]
Display: FID/PID INITIAL ATTEN
Entries are powers of 2: 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024. Larger values mean a smaller
output signal. INF (Infinite) means no signal out. The RCDR (recorder) and local printer/plotter
signals are affected. The INTEG (Integrator) and CMPTR (Computer) outputs are not attenuated.
The initial value stays effective until changed during the run by a time program for the same
detector, or by pressing the ATTENUATION keys. ATTENUATION keys override the method;
the method initial attenuation value is restored at RESET.
([BUILD/MODIFY]) [DETECTOR] (cont.)
Display:
FID/PID A/B INITIAL RANGE
FID/PID range entries are 8, 9, 10, 11, 12. Range 8 is the least sensitive, used when peaks are
very large; range 12 is the most sensitive, used to detect small signals. Each range is a power of
ten in output signal. For example, range 12 means 10E-12 Amperes of detected signal gives 1mV
deflection at the recorder (RCDR signal). Detector range setting effects RCDR, INTEG, and
CMPTR outputs.
Display:
FID/PID A/B AUTOZERO ON? NO
When auto zero is ON, an offset voltage is subtracted from the detector signal to give
approximately zero volts at each output. Thus, the initial chromatogram baseline is at electrical
55
zero of the recorder or data system.
Autozero is performed only when the GC is in STABILIZE or READY, or as a time programmed
event during RUN. The value of auto zero adjustment is displayed as BASELINE in mV by
pressing [STATUS] [DETECTOR] [ENTER]. The baseline value varies with detector range,
column type, temperature, and overall contamination, and can be used as an estimate of system
cleanliness.
Auto zero is automatically disabled during RUN to avoid zeroing out peaks. When initial auto
zero is turned off, the last auto zero offset value remains until the detector is turned off in the GC
Configure table.
Display:
TIME PROGRAM FID/PID A/B? NO
Time programs execute run time changes to detector parameter such as range and attenuation,
useful when different parts of the chromatogram have different peak sizes. Up to five time
programs can be built separately for each detector.
Display: PRGM l FID/PID A/B TIME IN MIN --Enter the time during the GC Run that the program is to be executed. Allowable entry is 0.01 to
650.00 minutes, or 0.01 minutes greater than the time in the previous program t0 0.01 minutes
less than the time in the following program. If the exact time is unknown, but is to be determined
during the chromatographic run, enter TUNE for the time and press [TUNE ON] during the run
to execute the program and record the time into the method. See the Special Uses of Keys
section for use of Tune mode.
([BUILD/MODIFY]) [DETECTOR] (cont.)
Display: PRGM 1 FID/PID A/B ATTEN x
Legal entries are the same as INITIAL ATTEN (see above). The displayed attenuation in the
entry field when a new step is added is the same value as in the previous program. Time
programmed attenuation modifications override updates made with the ATTENUATION keys.
Display:
PRGM l FID/PID A/B RANGE x
Legal entries are the same as INITIAL RANGE (see above). The displayed range in the entry
field when a new step is added is the same value as in the previous program.
Display:
PRGM 1 FID/PID A/B AUTOZERO? NO
Time programmed auto zero action during the run is useful to correct offset due to detector range
change or to bring a drifting baseline back on scale.
Display: ADD NEXT FID/PID A/B PROGRAM? NO
56
Up to five detector time programs can be added.
([BUILD/MODIFY]) [DETECTOR]
Display: TSD A/B INITIAL ATTEN
Entries are powers of 2: 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, and 1024. Larger values mean a
smaller output signal. INF (Infinite) means no signal out. The RCDR (recorder) and local
printer/plotter signals are affected;
The INTEG (Integrator) and CMPTR (Computer) outputs are not attenuated. The initial value
stays effective until changed during the run by a time program for the same detector, or by
pressing an ATTENUATION key. ATTENUATION keys override the method. The method
initial attenuation value is restored at RESET.
Display:
TSD A/B INITIAL RANGE
TSD range entries are 8, 9, 10, 11, 12. Range 8 is the least sensitive, used when peaks are very
large; range 12 is the most sensitive, used to detect small signals. Each range is a power of ten in
output signal. For example, range 12 means 10E-12 Amperes of detected signal gives 1mV
deflection at the recorder (RCDR signal). Detector range setting effects RCDR, INTEG, and
CMPTR outputs.
( [BUILD/MODIFY])[DETECTOR] (cont.)
Display:
TSD A/B AUTOZERO ON? NO
When auto zero is ON, an offset voltage is subtracted from the detector signal to give
approximately zero volts at each output. Thus, the initial chromatogram baseline is at electrical
zero of the recorder or data system.
Auto zero is performed only when the GC is in STABILIZE or READY, or as a time
programmed event during RUN. The value of the auto zero adjustment is displayed as
BASELINE in mV by pressing [STATUS] [DETECTOR] [ENTER]. The baseline value varies
with detector range, column type, temperature, and overall contamination, and can be used as an
estimate of system cleanliness.
Auto zero is automatically disabled during RUN to avoid zeroing out peaks. When initial auto
zero is turned off, the last auto zero offset value remains until the detector is turned off in the GC
Configure table.
Display:
TSD A/B BEAD POWER ON? NO
Press [YES] to enable bead current supply. Bead power is also time programmable.
Display:
TSD A/B BEAD CURRENT IN AMPS
57
Enter the desired bead current from 2.400 t0 3.800 Amperes. For extended bead life, use the
lowest current that will yield the required sensitivity for the analysis.
Display:
TIME PROGRAM TSD A/B? NO
Time programs execute run time changes to detector parameters, such as range and attenuation.
This is useful when different parts of the chromatogram have different peak sizes.
Display:
PRGM l TSD A/B TIME IN MIN ---
Enter the time during the GC Run that the program is to be executed. Allowable entry is 0.01 to
650.00 minutes, or 0.01 minutes greater than the time in the previous program t0 0.01 minutes
less than the time in the following program. If the exact time is unknown, but is to be determined
during the chromatographic run, enter TUNE for the time and press [TUNE ON] during the run
to execute the program and record the time into the method. See the Special Uses of Keys
section for use of the Tune mode.
([BUILD/MODIFY]) [DETECTOR] (cont.)
Display:
PRGM l TSD A/B ATTEN x
Legal entries are the same as Initial Attenuation (see above). The displayed attenuation in the
entry field when a new step is added is the same value as in the previous program. Time
programmed attenuation changes override updates made with the ATTENUATION keys.
Display:
PRGM 1 TSD A/B RANGE x
Legal entries are the same as Initial Range (see above). The displayed range in the entry field
when a new step is added is the same value as in the previous program.
Display: PRGM l TSD A/B AUTOZERO? NO
Time programmed Auto zero action during the run is useful to correct offset due to detector
range change or to bring a drifting baseline back on scale.
Display:
PRGM 1 TSD A/B BEAD POWER ON? NO
Time programmed bead power can be used to turn the bead off to prevent large solvent peaks
from overcooling and quenching or possibly cracking the bead. Turn the bead power back on in
the next time program after the solvent has eluted.
Display:
ADD NEXT TSD A/B PROGRAM? NO
Up to five detector time programs can be added.
([BUILD/MODIFY]) [DETECTOR]
58
Display:
ECD A/B INITIAL ATTEN
Entries are powers of 2: 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024. Larger values mean a smaller
output signal. INF (Infinite) means no signal out. The RCDR (recorder) and local printer/plotter
signals are affected. The INTEG (Integrator) and CMPTR (Computer) outputs are not attenuated.
The initial value stays effective until changed during run by a time program for the same detector,
or by pressing an ATTENUATION key. The ATTENUATION keys override the method. The
method initial attenuation value is restored at RESET.
([BUILD/MODIFY]) [DETECTOR] (cont.)
Display:
ECD A/B INITIAL RANGE
ECD range entries are l and 10. Range 10 is the least sensitive, used when peaks are very large.
Range l is ten times more sensitive, used to detect small signals. Detector range setting affects
RCDR, INTEG, and CMPTR outputs.
Display:
ECD A/B AUTOZERO ON? NO
When auto zero is ON, an offset voltage is subtracted from the detector signal to give
approximately zero volts at each output. Thus, the initial chromatogram baseline is at electrical
zero of the recorder or data system. Auto zero is performed only when the GC is in STABILIZE
or READY, or as a time programmed event during RUN, The value of auto zero adjustment is
displayed as BASELINE in mV by pressing [STATUS] [DETECTOR] [ENTER].
The baseline value varies with detector range, column type, temperature, and overall
contamination, and can be used as an estimate of system cleanliness. Auto zero is automatically
disabled during RUN to avoid zeroing out peaks. When initial auto zero is turned off, the last
auto zero offset value remains until the detector is turned off in the GC Configure table.
Display:
TIME PROGRAM ECD A/B?
Time programs execute run time changes to detector parameters such as range and attenuation.
This is useful when different parts of the chromatogram have different peak sizes. Up to five
time programs can be built separately for each detector.
Display:
PRGM x ECD A/B TIME IN MIN ---
Enter the time during the GC Run that the program is to be executed. Allowable entry is 0.01 to
650.00 minutes, or 0.01 minutes greater than the time in the previous program t0 0.01 minutes
less than the time in the following program. If the exact time is unknown, but is to be determined
during the chromatographic run, enter TUNE for the time and press [TUNE ON] during the run
to execute the program and record the time into the method. See the Special Uses of Keys
section for use of Tune mode.
59
([BUILD/MODIFY]) [DETECTOR](cont.)
Display:
PRGM l ECD A/B ATTEN x
Legal entries are the same as INITIAL ATTEN (see above). The displayed attenuation in the
entry field when a new step is added is the same value as in the previous program. Time
programmed attenuation changes override updates made with the ATTENUATION keys.
Display:
PRGM l ECD A/B RANGE x
Legal entries are the same as INITIAL RANGE (see above). The displayed range in the entry
field when a new step is added is the same value as in the previous program.
Display:
PRGM l ECD A/B AUTOZERO? NO
Time programmed Auto zero action during the run is useful to correct offset due to detector
range change or to bring a drifting baseline back on scale.
Display:
ADD NEXT ECD A/B PROGRAM? NO
Up to five detector time programs can be added. ( [BUILD/MODIFY] )
[DETECTOR] Enter
the GC Configure display TCD CARRIER GAS HELIUM? To set up correct filament control
and protection circuitry for Helium or Nitrogen carrier gas.
Display:
TCD A/B INITIAL ATTEN x
Entries are powers of 2: 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024. Larger values mean a smaller
output signal. INF (Infinite) means no signal out. The RCDR (recorder) and local printer/plotter
signals are affected. The INTEG (Integrator) and CMPTR (Computer) outputs are not attenuated.
The initial value stays effective until changed during run by a time program for the same detector,
or by pressing an ATTENUATION key. ATTENUATION keys override the method. The method
initial attenuation value is restored at RESET or run end.
Display:
TCD A/B INITIAL RANGE x
TCD range entries are 5, 0.5, and 0.05. Range 5 is the least sensitive, used when peaks are very
large. Range 0.05 is the most sensitive, used to detect small signals. Each range is a power of ten
in output signal. Detector range setting affects RCDR, INTEG, and CMPTR signal outputs.
( [BUILD/MODIFY])
Display:
[DETECTOR]
TCD A/B AUTOZERO ON? NO
60
When auto zero is ON, an offset voltage is subtracted from the detector signal to give
approximately zero volts at each output. Thus, the initial chromatogram baseline is at electrical
zero of the recorder or data system. Auto zero is performed only when the GC is in STABILIZE
or READY, or as a time programmed event during RUN. The value of auto zero adjustment is
displayed as BASELINE in mV by pressing [STATUS] [DETECTOR] [ENTER].
The baseline value varies with detector range, column type, temperature, and overall
contamination, and can be used as an estimate of system cleanliness. Auto zero is automatically
disabled during RUN to avoid zeroing out peaks,
When initial auto zero is turned OFF, the
last auto zero offset value remains until the detector is turned off in the GC Configure table.
Display:
TCD A/B FILAMENT TEMP OFF
Enter a filament temperature at least 50oC higher than the block DETECTOR TEMP, or enter 0
for OFF. The maximum FIL TEMP (filament temperature) that can be entered is normally 390oC.
If extremely high sensitivity is needed. The filament protection switch on the TCD controller can
be set to allow a maximum filament temperature entry of 490oC. Operation at these high filament
temperatures will greatly reduce filament lifetime. For minimum baseline drift and prolonged
filament life, it is best to operate with the lowest DETECTOR TEMP and FIL TEMP (i.e.,
minimum filament current) that will produce the required sensitivity. To display actual filament
current in mA, press [STATUS] [DETECTOR] [ENTER].
Display:
TCD A/B POLARITY POSITIVE? YES
Enter [YES] for most injections into the analytical (front) cell. If an injection is made into the
reference column of the TCD (rear cell) rather than the analytical column, or if a hydrogen peak
is detected, peaks will be negative or down-scale. Negative peaks are reversed by entering [NO].
For chromatograms containing both positive and negative peaks, polarity reversal can be time
programmed in the DETECTOR section of the method without additional hardware. Normally,
data acquisition systems require that all peaks have positive polarity.
( [BUILD/MODIFY])
Display:
[DETECTOR] (cont.)
TIME PROGRAM TCD A/B? NO
Time programs execute run time changes to detector parameters such as range and attenuation,
useful when different parts of the chromatogram have different peak sizes. Up to five time
programs can be built separately for each detector.
Display:
PRGM 1 TCD A/B TIME IN MIN ---
Enter the time during the GC Run that the program is to be executed. Allowable entry is 0.01 t0
650.00 minutes, or 0.01 minutes greater than the time in the previous program t0 0.01 minutes
less than the time in the following program. If the exact time is unknown, but is to be
determined during the chromatographic run. Enter TUNE for the time and press [TUNE ON]
during the run to execute the program and record the time into the method. See the Special Uses
61
of Keys section for use of Tune mode.
Display:
PRGM 1 TCD A/B ATTEN x
Legal entries are the same as INITIAL ATTEN (see above). The displayed attenuation in the
entry field when a new step is added is the same value as in the previous program. Time
programmed attenuation changes override updates made with the ATTENUATION keys.
Display:
PRGM 1 TCD A/B RANGE x
Legal entries are the same as INITIAL RANGE (see above). The displayed range in the entry
field when a new step is added is the same value as in the previous program.
Display:
PRGM l TCD A/B AUTOZERO? NO
Time programmed auto zero action during the run is useful to correct offset due to detector range
change or to bring a drifting baseline back on scale.
Display:
PRGM l TCD A/B POLARITY POS?
When both positive and negative peaks are present, the detector output polarity can be reversed
to yield all peaks with the same polarity. If a peak to be detected is negative, enter [NO] to
correct it.
Display:
ADD NEXT TCD A/B PROGRAM? NO
Up to five detector time programs can be added.
([BUILD/MODIFY])[DETECTOR]
If the FPD is operating in square root mode, the displays will show detector type SFPD. Only
displays for FPD are listed here, since keyboard entries for the two modes are so similar.
Display:
FPD A/B INITIAL ATTEN x
Entries are powers of 2: 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024. Larger values mean a smaller
output signal. INF (Infinite) means no signal out. The RCDR (recorder) and local printer/plotter
signals are affected. The INTEG (Integrator) and CMPTR (Computer) outputs are not attenuated.
The initial value stays effective until changed during run by a time program for the same detector,
or by pressing an ATTENUATION key. ATTENUATION keys override the method. The method
initial attenuation value is restored at RESET.
Display:
FPD A/B INITIAL RANGE x
FPD range entries are 8, 9, 10. Range 8 is the least sensitive, used when peaks are very large.
Range 12 is the most sensitive, used to detect small signals. Each range is a power of ten in
output signal. For example, range 9 means 10E-9 Amperes of detected signal gives 1mV
62
deflection at the recorder (RCDR signal). Detector range setting affects RCDR, INTEG, and
CMPTR outputs. SFPD ranges are 100, 10, and l, in order of decreasing sensitivity.
Display:
FPD A/B AUTOZERO ON? NO
When auto zero is ON, an offset voltage is subtracted from the detector signal to give
approximately zero volts at each output. Thus, the initial chromatogram baseline is at electrical
zero of the recorder or data system. Auto zero is performed only when the GC is in STABILIZE
or READY, or as a time programmed event during RUN. The value of auto zero adjustment is
displayed as BASELINE in mV by pressing [STATUS] [DETECTOR] [ENTER].
The baseline value varies with detector range, column type, temperature, and overall
contamination, and can be used as an estimate of system cleanliness. Auto zero is automatically
disabled during RUN to avoid zeroing out peaks. When initial auto zero is turned OFF, the last
auto zero offset value remains until the detector is turned off in the GC Configure table.
([BUILD/MODIFY])
Display:
[DETECTOR] (cont.)
TIME: PROGRAM FPO A/B? NO
Time programs execute run time changes to detector parameters such as range and attenuation,
useful when different parts of the chromatogram have different peak sizes. Up to five time
programs can be built separately for each detector.
Display:
PRGM l FPD A/8 TIME IN MIN ---
Enter the time during the GC Run that the program is to be executed. Allowable entry is 0.01 to
650.00 minutes, or 0.01 minutes greater than the time in the previous program to 0.01 minutes
less than the time in the following program. If the exact time is unknown, but is to be determined
during the chromatographic run, enter TUNE for the time and press [TUNE ON] during the run
to execute the program and record the time into the method. See the Special Uses of Keys section
for use of Tune mode.
Display:
PRGM l FPD A/B ATTEN x
Legal entries are the same as INITIAL ATTEN (see above). The displayed attenuation in the
entry field when a new step is added is the same value as in the previous program. Time
programmed attenuation changes override updates made with the ATTENUATION keys.
Display:
PRGM l FPD A/B RANGE x
Legal entries are the same as INITIAL RANGE (see above). The displayed range in the entry
field when a new step is added is the same value as in the previous program.
63
Display:
PRGM l FPD A/B AUTOZERO? NO
Time programmed auto zero action during the run is useful to correct offset due to detector range
change or to bring a drifting baseline back on scale.
Display:
ADD NEXT FPD A/B PROGRAM? NO
Up to five detector time programs can be added.
([BUILD/MODIFY])[DETECTOR]
Display:
HALL A/B INITIAL ATTEN x
Attenuation entries are powers of 2: 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024. Each factor of 2
doubles or halves the plotted signal amplitude. Larger values mean smaller signals. INF means
no signal out. Only the in-board printer/plotter signal is affected. Initial values stay effective until
changed during RUN by a time program for the same detector, or by pressing an
ATTENUATION key, which will override the method. The method initial attenuation is restored
at RESET or run end.
Display:
HALL A/B VENT OPEN? YES
The Hall detector solvent vent is normally open until the solvent has passed the detector during
the run. The vent is then closed with a detector time program. Enter [YES] to open the vent or
[NO] to close it.
Display:
TIME PROGRAM HALL A/B? NO
Time programs execute run time changes to plotter attenuation or solvent vent.
Display:
PRGM l HALL A/B TIME IN MIN ---
Enter the time during the GC run that the program is to be executed. Allowable entry is 0.01 to
650.00 minutes, or 0.01 minutes greater than the time in the previous program to 0.01 minutes
less than the time in the following program. If exact time is unknown, but is to be determined
during the run, enter TUNE for the time and press [TUNE ON] during the run to execute the
program and record the time into the method. See the Special Uses of Keys section for use of
Tune mode.
Display:
PRGM 1 HALL A/B ATTEN x
Legal entries are the same as INITIAL ATTEN (see above). The displayed attenuation in the
entry field when a new step is added is the same value as in the previous program. Time
programmed attenuation changes override updates made with the ATTENUATION keys.
Display:
PRGM 1 HALL A/B VENT OPEN? NO
The Hall detector vent can be opened or closed at any time during the run. Enter [YES] to open
64
the vent, or [NO] to close it.
Display:
ADD NEXT HALL A/B PROGRAM? NO
Up to five detector time programs can be added.
DETECTOR STATUS Displays
16. ([STATUS] [DETECTOR]
Display: DET aaao SET sss or OFF
The actual detector temperature followed by the active method set point value is displayed by
pressing [STATUS] [DETECTOR]. When the actual temperature is out of tolerance of its set
point value, the degree symbol flashes, the NOT READY light is ON, and a detector temperature
error message results if the GC is in RUN. Press [ENTER] to view additional detector Status
displays.
Display:
FID/PID A/B BASELINE xxxxx.xx MV (A/Z)
BASELINE is the applied auto zero correction in mV. The value decreases by a factor of ten with
each decrease in detector range. A/Z is displayed only when auto zero is actually ON. When auto
zero is OFF, this field is blank. See description of FID A AUTOZERO ON? above.
Display:
FID/PID A/B ATTEN aaaa RANGE xxx
The FID/PID is operating at attenuation aaaa and range 10E-xxx A/mV. The display updates to
reflect run time changes in detector status.
Display:
DET A/B OFF
Detector A has been turned off via TURN HARDWARE ON-OFF? In the GC Configure table.
This will be the only detector status display, but the detector method section can be fully
displayed and edited.
([STATUS]) [DETECTOR]
Display:
ECD A/B BASELINE xxxxx-xx MV (A/Z)
BASELINE is the applied auto zero correction in mV. The value decreases by a factor of ten with
detector range change from 1 to 10. A/Z is displayed only when auto zero is actually ON. When
auto zero is OFF, this field is blank. See description of ECD A AUTOZERO ON? Display.
Display:
ECD A/B ATTEN aaaa RANGE xxx
The ECD is operating at attenuation aaaa and range xxx. The display updates to reflect run time
65
changes in detector status.
( [STATUS] )
Display:
[DETECTOR]
DET A/B OFF
Detector A has been turned OFF via TURN HARDWARE ON-OFF? in the GC Configure table.
This will be the only detector status display, but the detector method section can be fully
displayed and edited.
([STATUS]) [DETECTOR]
Display:
TCD A/B BASELINE xxxxx.xx MV (A/Z)
BASELINE is the applied auto zero correction in mV. The value decreases by a factor of ten with
each decrease in detector range. A/Z is displayed only when auto zero is actually ON When
auto zero is OFF, this field is blank.
See description of TCD x AUTOZERO ON? in HELP 15 TCD
Once the block and filament temperatures have equilibrated, balance the TCO bridge: set
detector range to 0.05, auto zero ON, and adjust the TCD balance knob on the TCD PC Board
until the displayed baseline is close to zero.
Display:
TCD A/B ATTEN aaaa RANGE xxx
The TCD is operating at attenuation aaaa and range xxx. The display updates to reflect run time
changes in detector status.
Display:
TCD A/B FIL TEMP sss CUR sss MA
The TCD filament temperature set point in the active method is displayed
actual resultant filament current in mA.
Display:
TCD A/B POLARITY POSITIVE
(NEGATIVE)
Status of current TCD polarity reversal mode.
programs.
Display:
followed by the
Display updates if polarity reversed by time
DET A/B OFF
Detector A has been turned OFF via TURN HARDWARE ON-OFF? In the GC Configure table.
This will be the only detector status display, but the detector method section can be fully
displayed and edited.
66
([STATUS]) [DETECTOR]
Display:
TSD A/B BASELINE xxxxx.xx MV (A/Z)
BASELINE is the applied auto zero correction in mV. The value decreases by a factor of ten with
each decrease in detector range. A/Z is displayed only when auto zero is actually ON. When auto
zero is OFF, this field is blank. See description of TSD x AUTOZERO ON? in HELP 15 TSD
Display:
TSD A/B ATTEN aaaa
RANGE xxx
The TSD is operating at attenuation aaaa and range 10E-xxx A/mV (integrator output). The
display updates to reflect run time changes in detector status.
Display:
TSD A/B s.sss AMP vv.vVOLT BIAS
This status display shows the TSD bead current set point and the actual negative bias voltage.
Bias voltage is conveniently set by turning the TSO Bias slot adjustment (R34) on the FID/TSD
PC Board while observing this display.
Display:
DET A/B OFF
Detector A/B has been turned OFF via TURN HARDWARE ON-OFF? In the GC Configure
table. This will be the only detector status display, but the detector method section can be fully
displayed and edited.
([STATUS])[DETECTOR]
Display:
FPD A/B BASELINE xxxxx.xx MV (A/Z)
BASELINE is the applied auto zero correction in mV. The value decreases by a factor of ten with
each decrease in detector range. A/Z is displayed only when auto zero is actually ON. When auto
zero is OFF, this field is blank. See description of FPD A/B AUTOZERO ON? in HELP 15 FPD.
Display:
FPD A/B ATTEM aaaa
RANGE xxx
The FPD is operating at attenuation aaaa and range I0E-xxx A/mV (integrator output). The
display updates to reflect run time changes in detector status.
Display:
Display:
FPD A/B PM TUBE xxx VOLTS
SFPD A/B PM TUBE xxx VOLTS
The FPD photomultiplier tube voltage is displayed with 10 volt resolution.
([STATUS]) [DETECTOR]
Display:
DET A/B OFF
Detector A/B has been turned OFF via TURN HARDWARE ON-OFF? in the GC Configure
table. This will be the only detector status display, but the detector method section can be fully
67
displayed and edited.
([STATUS]) [DETECTOR]
Display:
Display:
HALL A/B VENT OPEN (CLOSED)
HALL A/B ATTEN aaaa VENT OPEN (CLOSED)
The display updates to show run time changes in attenuation or solvent vent state. Attenuation is
only displayed when the PLOTTER section is in the active method.
PLOTTER Method Section
17. [PIOTTER]
Display:
ADD PLOTTER Section? NO
Provides the opportunity to include plotting and/or printing report and run log as part of the
method. The active method must have a PLOTTER section to plot with the [(PLOTTER) START]
key.
Display:
INITIAL PLOT SPEED IN CM/MIN
Enter the desired plotting speed from 0 (Off) to 30.0 cm/minute. Plotting begins when (START)
PLOTTER is pressed or an injection made.
Display:
ZERO OFFSET IN PERCENT
Enter the plotter electrical zero offset in percent. Range is 0 to 100 percent offset. Zero percent
offset is at the right hand paper edge.
Display:
PLOT SIGNAL A OR B?
Choose the detector signal to be plotted by entering [A] or [B]. The detector analog outputs of
the instrument are not affected.
Display:
TIME TICKS? NO
Time ticks are printed along the right hand edge of the paper and are synchronized with GC Run
time. Ticks are spaced I minute apart for plot speeds of I cm/min or faster, otherwise they are 5
minutes apart.
[PLOTTER] (cont.)
Display: INSTRUMENT EVENT CODES? NO
Codes annotate instrument events (changes to the GC) along the plot as they occur. The lower
edge of the code letters are aligned with the event time. A description of these codes is contained
in Table l in the printer/Plotter section. For IBDH unique annotations, refer to Figure 4 in the
IBDH Operation section. Zero offset must be at least 15% to allow space for printing
annotations.
68
Display:
FIRST USER NUMBER?
Enter any number up to four digits as the first part of the user number to be printed on methods
and reports.
Display:
LAST USER NUMBER? ----
Enter any number up to four digits that will be printed as the second part of the user number.
Display:
PRINT USER NUMBER? NO
When the method report is printed, the two part hyphenated user number is printed beside the
GC method number. For example METHOD 1 324-6999, where 324-6999 is the user number.
This is useful to label the method with analytical process number or operator identification.
Display:
PRINT REPORT AT RUN END? YES
If entry is YES, then the run report will be printed at the run end. The report contains information
such as method number, time of injection, run number, and Auto Sampler rack and vial number.
If entry is NO, the report will be suppressed, but may be printed for the most recent run by
pressing [SHIFT] [REPORT].
Display:
PRINT RUN LOG AT RUN END? NO
The Run Log lists actual initial conditions at the time the GC went to run, injection, all
instrument changes during run, and run end or reset To print the Run Log along with the method
report when the run ends, enter [YES].
NOTE: The Run Log is only printed if PRINT REPORT AT RUN END? Is also YES.
The Run Log is always recorded. If printout was not requested in the most recent GC run and is
now wanted, change PRINT REPORT AT RUN END? To YES and print the report and Run Log
by pressing [SHIFT] [REPORT].
[PLOTTER](cont.)
Display:
TIME PROGRAM PLOTTER?
NO
Time programs automatically execute changes in plot speed or which detector, A or B, is plotted
during the GC run. Enter YES to add up to five plotter time programs to the method.
Display:
PRGM l PLOTTER TIME IN MIN ---
Enter the time during the GC Run that the program is to be executed. Allowable entry is 0.01 to
650.00 minutes, or 0.01 minutes greater than the time in the previous program to 0.01 minutes
less than the time in the following program. If exact time is unknown, but is to be determined
during the chromatographic run, enter TUNE for the time and press [TUNE ON] during the run
to execute the program and record the time into the method.
69
See Special Uses of Keys section for use of Tune mode.
Display:
PRGM l PLOT SPEED IN CM/MIN
It is useful to change plot speed during the run when only widely separated regions of the
chromatogram have peaks of interest. Enter desired plot speed, from 0 (off) to 30.0 cm/min. The
default plot speed when plotter programs are added is the speed in the previous step.
Display:
PRGM l PLOT SIGNAL A OR B?
Choose detector signal A or B to be plotted at this program time. The default signal when
programs are added is the same as in the previous program. The detector analog outputs are
unaffected.
Display:
ADD NEXT PLOTTER PROGRAM? NO
Enter YES to add another time program to the method PLOTTER section. Up to 5 time programs
can be built
([STATUS]) [PLOTTER]
18. Display:
PLOT ddd A/B AT vv.v CM/MIN ZERO xxx
The default plotter parameters are to plot detector A/B at 1 cm/min with a 10 percent zero offset.
These plot parameters are valid and displayed even when not actually plotting. The displayed
parameters are updated when time programmed plotter changes occur during the run.
Display: NO PLOTTER SECTION IN METHOD
Active method does not contain PLOTTER method section.
19. RELAY Method Section
( [BUILD/MODIFY] ) [RELAY]
Display:
ADD RELAY SECTION?
NO
Enter [YES] to add the RELAY (external events) control section to the method. The External
Events PC Board must be present. Four relays and 20 time programmable events are available
to control: 4 AC switches to control sampling valves (relays l, 2, 3, 4), one low level contact
closure (simultaneous action with relay 4), one signal switching relay (simultaneous action with
relay 3). See the tabbed section
Relays for additional details and required connections.
Display:
INITIAL RELAYS xxxx
Enter positive numbers to turn relays ON, negative numbers to turn them OFF. Example:
1-2-34 turns relays 1 and 4 0n, 2 and 3 off. It is only necessary to enter the relay numbers that
require a change of state.
Relays remain at their set point until they are changed by the
active method. See also INITIAL RELAYS AT RUN END? Display in the SET CHECKS FOR
70
GC READY? Category in the GC Configure table.
( [BUILD/MODIFY])
Display:
[RELAY]
(cont.)
TIME PROGRAM RELAYS?
NO
Time programs execute changes to the relays during the GC run, for example, to open and then
close a sampling valve for injection.
Display:
PRGM 1 RELAY TIME IN MIN ---
Enter the time during the GC Run that the program is to be executed allowable entry is 0.01 to
650.00 minutes, or 0.01 minutes greater than the time in the previous program to 0.01 minutes
less than the time in the next program. If the exact time is unknown, but is to be determined
during the chromatographic run, enter TUNE for the time and press [TUNE ON] during the run
to execute the program and record the time into the method. See the Special Uses of Keys section
for use of Tune mode.
Display:
PRGM l RELAYS xxxx
Allowable entry is the same as initial relays. The value in the entry field when a new program is
added is the same as in the previous program.
Display:
ADD NEXT RELAY PRGM? NO
up to 20 relay time programs can be added
20. ( [STATUS]) [RELAY]
Display:
RELAYS ON xxxx
Relays currently ON are displayed as positive numbers I to 4.
Display:
NO RELAY SECTION IN METHOD
The active method contains no RELAY section. Relays may be ON if they were left on from a
previous method.
21. Display: METHOD COMPLETE - END TIME xxxxx
This is the last display when a method is built. The method end time in minutes is equal to the
longest time required by an event in the method, either a temperature program or hold time, or a
time program. TUNE time entries are not considered.
Method end time can also be displayed by pressing [STATUS] then the appropriate method
number key.
22. SEQUENCE TABLE Displays
([BUILD/MODIFY]) [SEQUENCE TABLE]
71
The Sequence Automation table is a list of methods to be run in order. See the Automation
Control section.
RUNNING SEQUENCE AUTOMATION:
- Sample injection can be facilitated with time programmed relay control of a sampling valve
- Press [BUILD/MODIFY] [SEQUENCE TABLE]; follow the displays (see below). Specify in
order in each program the next method to be run and the number of times to run it.
- Press [ACTIVATE] [SEQUENCE TABLE] to set up for automated mode.
Press [START] to begin automation. The GC goes to RUN at GC READY '
- Sequence automation continues until RESET is pressed, or after last
SEQUENCE TABLE prgm, if SINGLE pass, or when A/S stop pin is found.
deactivated.
Table is then
([BUILD/MODIFY]) [SEQUENCE TABLE] (cont.)
Display:
RUNS OF TABLE?
SINGLE (MUFTI)
Enter SINGLE and SEQUENCE TABLE will be executed once during the automation and then
stopped. For MULTI runs, the table will be repeated from the beginning until RESET is pressed
Display: STOP AUTOMATION AFTER ERROR
See same display under HELP number 24
Display: PRGM n RUN METHOD
Enter the first method number to be run in the automation sequence. Use ENTRY keypad.
(METHODS keys do not work here and entry field cannot be left blank).
Display: PRGM n NUMBER OF RUNS--The number of times the method is to be run before automation proceeds to the next programmed
method.
Display: ADD NEXT SEQ TABLE PROGRAM? NO
YES-allows addition of up to 16 sequence table programs.
Display: SEQ TABLE COMPLETED
The Sequence Automation Table has been built and contains at least one program. Press an
OPERATIONS key for the next desired action.
Display: TABLE SUSPENDED
Automation can be temporarily interrupted during sample purging,while in run, or at the end of
the current run by pressing [SHIFT] [(RACK TABLE) SUSPEND]. Use to pause automation to
72
modify the table or methods, or to change sample vials and has a less drastic effect than pressing
[RESET]. Resume automation by pressing [START].
Automation is terminated if suspend is presses during sample sizing or injection. Press RESET to
clear suspension. The sequence or rack table may need to be modified to continue the method for
the next injection.
23. ([STATUS) [SEQUENCE TABLE]
Display: PRGM x RUN nn INJ nn
Sequence automation is active and the light by the Sequence Table key is ON. The current
program number and number of runs of that program are displayed.
Display: SEQ TASL.E VOT ACTIVE
Sequence automation is not in progress. The table is deactivated at automation end.
Display: SEQ AUTOMATION SUSPENDED
The ongoing Sequence automation has been interrupted with the SUSPEND action. Current
Sequence Table program Status can be displayed by pressing ENTER. Resume automation
where it left by pressing START.
Automation is terminated if suspend is pressed during sample sizing or injection. Press RESET
to clear suspension. The sequence or rack table may need to be modified to continue the method
for the next injection.
24. GC CONFIGURE Displays
Display: SET TIMES OR DATE? NO
Enter [YES] to access the time/date category of GC Configure displays.
Display: THERMAL STABILIZE TIME
This time provides an equilibration period for the GC to reach a stable thermal state after one or
more zones have changed temperature. It applies to all temperature zones in use. When all GC
thermal zones are in tolerance
of the method setpoint values, the stabilization countdown begins and can be observed in Status.
The GC NOT READY light stays ON. Pressing [RESET] will end the STABILIZE period, and
the GC will show READY.
A stabilize time of 2 minutes is adequate for most operations. If large temperature changes are
made as part of a method, a 1onger period may be desired.
Display:
ENTER TIME OF DAY IBS NHMM xxxx
This is a 24-hour clock, so, if it is 2:25 pm, press [1] [4] [2] [5] [ENTER]. Once set, time is
maintained unless the GC is turned off or a power failure occurs.
GC CONFIGURE Displays (cost.)
Display: ENTER DATE- DAY MONTH XXXX
73
Enter the present day and month as a 4-digit number with no
punctuation. Use zeros to fill spaces. For example, enter 0503 for 5
March.
Display: ENTER DAT - YEAR xx
Enter the last two digits for the current year.
Display:
COOLANT TIME OUT IN MIN INF.
Provides for coolant conservation if coolant is being used for either the column or injector zones.
Enter the time that the GC waits during STABILIZE/NOT READY or after READY before
automatically shutting off the coolant to both zones if the GC does not go to RUN. The timer is
also reset and reactivated when the GC advances from STABILIZE/NOT READY to READY.
Enter INF(infinite time) and the coolant will never be timed out. Once coolant has timed out,
press [RESET] to re-enable coolant and restart the coolant timer.
Coolant use is enabled via TURN HARDWARE ON - OFF in the
GC Configure table. Note: Coolant tine out should be set longer than the expected
STABILIZE/NOT READY or READY times so that the cooled zone will remain ready
during STABILIZE/NOT READY or READY.
25. Display: SET TEMP LIMITS? NO
Enter [YES] to access displays where protective upper temperature limits for each thermal zone
are set. The temp limits for column,injector,auxiliary,and detector ovens are entered
separately. The upper value in prompts for method temperature displays will be
adjusted so that temperatures higher than the respective TEMP LIMITS cannot be entered.
Temperature limits can be raised at any time, but cannot be reduced below the highest
temperature for that zone in the method. This will be reflected in the lower value of the prompt
for each TEMP LIMIT display. The minimum temperature limit is 50oC.
Display: COLUMN TEMP LIMIT
Enter a safe upper temperature 1imit for the columns currently installed. Do not exceed the
highest operating temperature recommended by the column manufacturer.
GC CONFIGURE Displays (cont.)
Display: INJECTOR A/B TEMP LIMIT
The maximum safe temperature for packed column injectors
0r the split/splitless capillary injector is determined by the septum and column installed. The
temperature should not be so high that degradation and/or excessive bleed can occur. The
on-column capillary injector is limited to a maximum of 3500C.
74
Display: AUXILIARY TEMP LIMIT
See HELP13 for a description of the auxiliary thermal zone.
Display: DETECTOR A/B TEMP LIMIT
The maximum safe operating temperature of the detector is limited by the column that is inserted
into the detector and by the ferrules in use. Vespel R ferrules may crack at temperatures
over320oC; graphite ferrules can withstand 4200C.
Display: COLUMN STANDBY TEMP?
The allowable range for. the COLUMN STANDBY TEMP is from 20oC to the COLUMN TEMP
LIMIT. See next display.
26. Display: ENABLE COLUMN STANDBY TEMP? NO
Upon entry of [YES], the COLUMN STANDBY TEMP entered in the previous display will
override the active initial column temperature at run end or automation end. This is intended for
use when it is undesirable to leave the column at the initial column temperature of the active
method for 1ong periods of time and no user is present to adjust the active method temperature.
When the column oven is held at the standby temperature, the GC is held NOT READY and
STANDBY is displayed in column status. Press [RESET] to re-enable column temperature
control by the active method.
Display: SET CHECKS FOR GC READY? No
Enter [YES] to access the category of displays allowing selection of certain GC functions to be
watched or ignored in GC READY criteria. When a WAIT FOR…entry is NO, that zone need
not be within the required tolerance band of its method setpoint for the GC to become READY.
This offers flexibility when particular hardware is present but is not being used in the current
analysis. Since the zone is not checked for READY, no Error Log entry is generated during
RUN.
GC CONFIGURE Displays (cont.)
Display: SET CHECKS FOR GC READY?
NO (cont.)
Note: Column oven temperature must be at its setpoint for the GC to
become READY. The displays are:
WAIT FOR INJ A/ B TEMP READY?
WAIT FOR AVX TEMP READY?
WAIT FOR DET A/B TEMP READY?
WAIT FAR FLOW READY? YES
WAIT FOR SPLIT RATIO READY?
WAIT FOR VELOCITY READY?
WAIT FOR PRESSURE READY?
These displays are only in instruments with electronic flow and/or pressure readout. Velocity
refers to the average linear velocity of the carrier gas.
Display: WAIT IF PAPER OUT? YES
YES presents GC READY if inboard printer/plotter is out of paper.
75
Display: WAIT FDR EXT DEVICE READY? NO
YES holds the GC NOT BEADY until a ready signal is received from some external device(such
as a purge and trap unit). See the tabbed section External Data Systems for cable connections.
Display: WAIT IF FAULTS PRESENT? NO
Enter YES and the GC will be held NOT READY if background faults are defected. Thus,
automation does not continue if faults occur.
27. Display: INITIAL RELAYS AT RUN END? YES
The External Evens (relay) PC Board must be installed This entry determines when the relays
return to the initial relay conditions of the active method after time programmed changes during
the run. A YES entry causes the relays to set to their initial conditions immediately at run end or
RESET. A NO entry holds the relays in their existing state until the GC is READY and the
READY light in ON. If operating in the splitless capillary mode, enter NO..
Display: TURN HARDWARE ON-OFF? NO
YES accesses a set of displays to individually turn selected hardware ON and OFF. Hardware
turned off has no power supplied to the zone,but methods remain unchanged. Status displays for
each show setpoints OFF.
GC CONFIGURE Displays (cont.)
Display: DETECTOR A ON? YES
DETECTOR B ON? YES
The detector must be turned on to get a chromatogram; however,it is recommended to turn it off
before working on the tower. When the detector is turned off, the autozero offset (BASELINE
value in DETECTOR Status) is cleared to zero until the next autozero action. The following
specify detector functions are turned off: FID polarizing voltage, TSD bead current and bias
voltage, TCD filament current, ECD pulse voltage and FPD PM tube high voltage
Displays:
DETECTOR A/B OVEN? YES
INJECTOR A/B OVEN?
YES
AUXILIARY OVEN ON? YES
NO interrupts heater power to the indicated zone. Turning the capil1ary on-column injector oven
OFF also turns off its cryogenic coolant valve. Column oven control is turned on (COL OVEN
ON) or off (SHIFT, COL OVEN OFF), via a key in the GC CONTROL keyboard section.
Displays:
COOLANT TO COLUMN?
NO
COOLANT TO INJECTOR? NO
YES enables the cryogenic, valve for the column oven or capillary on-column injector. If only
one valve is present, the display for the other is held invisible. Column actual temperature must
be 1ess than 50oC for coolant to come an. On-column capillary injector coolant is supplied
whenever needed to quickly lower or maintain temperature.
76
NOTE: For optimum temperature control and fastest cool down of the column oven below
500C(near ambient), do not enable column coolant if cryogenics is not present (column
temperature and vent control are different).
The COOLANT TIME OUT can be used to conserve coolant by shutting off all coolant valves at
the specified time after GC READY. See GC Configure category SET TIMES OR DATE?
28. Display: SET COLUMN PARAMETERS? NO
Enter YES to access displays related to e1ectronic flow readout. This data is combined with
pressure transducer readout to calculate column flow, column average linear velocity, and split
ratio, all displayed in Column STATUS.
Display: COLUMN A/B INSTALLED?
Enter YES for column A or B that is in use. This enables displays in the COLUMN section of the
method and Column STATUS related to pressure and flow.
Display: COLUMN A/B LENGTH IN METERS
Enter the column length in meters. If the 1ength of a coiled column is unknown, it can be
estimated by mu1tipiying the(coil diameter) × (pi) × (the number of loops in the coil). (Use
pi= 3.14.)
Display: COLUMN A/B ID IN MICRONS
Enter the internal diameter of the column in microns. If it is unknown, measure the flow with a
bubble flowmeter and adjust the value unti1 the display equals the value measured. This will give
you the actual diameter.
Display: COLUMN A/B CARRIER GAS
Specify carrier gas in use: HE,N2 OR H2, by using the values shown when the PROMPT key is
pressed.
Display: OTHER CONFIGURATIONS?
Enter YES to access a miscellany of displays depending on the hard ware present.
Display: A/S SLOW INJ RATE IN UL/SEC
Injection rate can affect peak shape. Enter rates in microliters/second to correspond to the fast and
slow settings.
FAST rate is appropriate for most packed column and split infections、and for small capillary
on-column injection volumes (<1 microliter ). The preset fast rate of 5 ul/sec is recommended for
most applications.
SLOW injection rate is used for capillary splitless injections,and for large capillary on-column
77
injectionvolumes(>l microliter) . A slow injection rate of 1.0 ul/sec is useful for most cases.
GC CONFIGURE Displays (cont.)
Display: TCD CARRIER GAS HELIUN? YES
Enter YES for helium or hydrogen, NO for nitrogen. This establishes the correct filament
temperature vs. voltage calculations for operation of the TCD-equipped instruments.
Additionally,if entry is YES(helium carrier),protective circuitry turns the TCD filaments off
after 4 minutes if poor flow or a large air (nitrogen) leak is detected. With entry of NO (nitrogen
carrier gas), this filament protection is disabled. The action of the filament temperature limit
switch is not affected (see the TCD detector section).
NOTE: Use of nitrogen with the TCD is not recommended because sensitivity is much
lower than with helium/hydrogen and the fi1aments must be operated at high temperatures
where filament life is significantly shortened and filament protection must be disabled.
Display: NEXT RUN NUMBER? nnnn
The run number to be printed on the report of the next GC run can be changed and the run
number counter increments from there. The next run number can be from 1 to 9999, as shown
in the prompt.
Display: SOUND KEYSTROKES? YES
Allows keystroke "beep" to be turned off or on.
Display: SIGNAL SWITCHING TO DS? NO
Refers to serial I/0 control station feature. A single analog channel to the data system can be
switched between GC detectors A and B under method control if YES is entered. This adds
displays to the Detector A method section for designating the detectors and times when they are
to be switched. The Serial I/0 PC Board must be installed. Refer to the tabbed section External
Data Systems for board instal1ation.
Display:
SET LOCK CODE?
NO
YES allows the instrument lock code to be modified by the user,provided he knows the existing
code. This only enters the code to be used for locking, and does not itself lock anything. The
code is used with the LOCK/UNLOCK key in the OPERATIONS section of the keyboard to
protect methods, automation, and GC Configure tables one-at-a-time. Locked methods and
tables cannot be modified. (The TURN HARDWARE ON-OFF section of the GC Configure
table remains unlocked). Both lock and unlock operation, require know1adge of the code. See
HELP 5.
GC CONFIGURE Displays (cont.)
Display: CURRENT LOCK CODE [] [] [] []
The current lock code must be re-entered before the code can be changed. An incorrect code will
be flagged as an illegal entry. The preset code on instrument power-up is 0
78
Display: NEW LOCK CODE xxxx
A11owable 1ock code is 0 to 9999. Verify that the new code is correctly typed before pressing
ENTER, since displaying it again requires knowing it. Do not forget the code: the intended
recovery procedure is instrument cold start, in which case all methods and tables are lost.
29. Display: GC CONFIGURE TALE COMPLETE
This is the last display in the GC Configure table. Select the next desired operation.
34 Series Miscellaneous Operations
30. Display: INCOMPLETE PROGRAM - DELETE IT? NO
This display is only seen when adding new programs to a method section or automation table.
Attempt has been made to change the display before a valid entry of time, temperature, or rate.
The program is considered incomplete. Only displays with blank entry fields cause this display.
Display: ILLEGAL ERTRY - PRESS PROMPT
Illegal entries are not allowed. Press and hold [PROMPT] to view legal entries for each
parameter. When PROMPT is released, the original display with the illegal value is displayed
again. Displayed Prompt values (e.g., temperature ranges) can change and are updated
as other entries are made.
Note: If the field contains an illegal value and a key other than ENTER is pressed that
changes the display, the previous legal value is retained.
31. 34 Series Miscellaneous Operations (cont.)
Display: METHOD LOCKED or TABLE LOCKED
Methods, Rack Table, Sequence Table, or the GC Configure table will not accept entries if they
are locked. They may be completely examined, but parameters cannot be changed.
Display: ILLEGNL SECTION
A fault condition exists. For example, a pc board was removed after the method section was built.
Certain operations on illegal sections are not allowed. Either correct the hardware or delete the
section. If the illegal section is in the active method,the STATUS light blinks and the GC is held
NOT READY. Press [STATUS] to display the fault and refer to the
Diagnostics/Troubleshooting Section.
79
32. Displays: TEST OK
FAULT XXX
RETURN TO TEST MENU? NO
VENT TEST?
RELAY TEST?
KEY ECHO TEST?
DESTRUCTIVE RAM TEST?
TEST SESSION COMPLETED
INSTR BUSY-CANNOT ENTER TEST
These displays and a few others are part of instrument diagnostics,initiated by pressing
[SHIFT][INSTR TEST]. Instrument test is automatically performed on cold start. See the
Diagnostics/Troubleshooting section for use of diagnostics.
33. Display: POWER FAIL/WARM START OCCURRED
Instrument power was lost,but battery back-up saved all methods and tables. Update the time
and date in the GC Configure table. The display remains until RESET is pressed.
34. INTEGRATION Displays
Display: ADD INTEGRATION SECTION?
The INTEGRATION section serves two purposes: (1) it allows you to enter calculation
parameters such as run mode, amount standard, sample ID, etc., to be used by the method when
you are not using an Auto Sampler and Sample Table, and (2) it also stores information such as
calculation type, report format, whether or not to subtract the stored blank baseline, etc., which do
not change from run to run in an automated sequence and thus do not need to be included in the
Sample Table
Deleting either the TIME EVENTS or INTEGRATION section will delete both sections from
the method.
Display: RUN MODE?
See HELP 19,display PRGM n RUN MODE. The run mode entered here will be used for al1
vials not identified by rack and vial numbers in the sample table.
Display: PEAK MEASUREMENT PARAMETER?
The value entered here (1=peak area; 2=peak height; or 3= square root of peak height) determines
what measure of peak size will be used calibration and analysis runs. While it is possible to
calibrate a method using peak heights and then change the method to calculate analysis results by
peak area, the results would have very little meaning.
80
In general,peak area is most useful in quantitating well-resolved peaks, while peak height may be
more reliable for poorly resolved or low level peaks. The square root of height mode is only
useful in correcting for the square law response of the flame photometric detector (FPD) to sulfur
compounds. Refer to the FPD section of this manual for more details on square root mode.
INTEGRATION Displays (cont.)
Display: LONG REPORT FORMAT?
Enter [YES] to print the long report, which includes all information found in the short report as
well as information on the peak widths fond, retention time offsets, total unidentified peak area
or height, and relative retention times.
The long report is very useful during method development, as everything the system has
determined about the chromatogram is printed out. The short report is usually chosen for more
routine analyses, when brevity is desired.
Display: RESULT CALCULATION TYPE
Enter the calculation type:
1=A% ( Area Percent)
2=N% ( Normalized Area Percent)
3=IS
(Internal Standard)
4=ES
(External Standard)
Area Percent(A%) is the simplest type calculation, as it does not require identification of peaks
in the chromatogram, calibration standards, or anything beyond a suitably prepared sample and
adequate detector sensitivity. The area or height of all selected. Peaks are summed, and the
amount of each individual peak is expressed as a percentage of this sum in the report. A% is a
useful way of presenting data on all peaks in a chromatogram,known or unknown. At is not
suitable for most quantitative applications, as an increase in the percentage of any one peak
results in a relative decrease in percentage of all other peaks.
Normalized Area Percent (N%) is similar to A% except that the area of each identified peak is
multiplied by a response factor before the summation and determination of percentages. This
allows for variations in detector response to different compounds in the sample. The factors can
be entered into the Peak Table by hand if they are known or, more commonly, are determined by
running a standard in a calibration run mode. Refer to the IBDH Operation section for details.
INTEGRATION Displays (cont.)
Display: RESULT CALCULATION TYPE (cont.)
The Internal Standard (IS) calculation type requires the addition of a standard substance to the
sample to serve as an internal yardstick for detector response against which the sample peaks can
be compared. The standard must be pure, easily resolved from all sample components,never
81
occur naturally in the sample, and, ideally, should behave very similarly to the sample
components during the preparation of the sample, e.g. extraction or derivatization.
IS can be the most reliable mode of analysis, but it also requires the most time for method
development.
The External Standard (ES) calculation type relies on the injection of known amounts of
standard compounds to calibrate the detector response. Once these calibration factors have been
determined and stored in the Peak Table,subsequent injections in the analysis mode combine the
peak area or height information from the chromatogram with the calibration factor information
from the Peak Table to calculate the amount of each identified peak present。
The precision obtained by ES depends greatly on the stability of the chromatographic system and
the injection technique used. ES calculation type is particularly suited to automated operation.
Display: DIVISOR
See HELP 19, display PRGM n DIVISOR.
Display: AMOUNT STANDARD
See HELP 19, display PRGM n AMOUNT STANDARD.
Display: MULTIPLIER
See HELP 19, display PRGM n MULTIPLIER.
Display: RESULT UNITS
Enter an 8 character header to be printed above the result column in the report. This has no effect
on the calculations.
INTEGRATION Displays (cont.)
Display: REPORT UNIDENTIFIED PEAKS
Unidentified peaks are those which are detected by peak processing,are not rejected by peak
reject or solvent reject, and are not listed in the current Peak Table.
If you request that unidentified peaks be reported (by entering [YES]), each peak that is not
listed in the Peak Table will be multiplied by the unidentified peak factor. (UPF,see below)and
the result reported in chronological order along with the identified peak results.
If you request NO unidentified peaks, their retention times,areas/heights, etc. will not appear
individually in the report. Only the total unidentified peak area/height for the chromatogram as a
whole will be reported.
If there is no Peak Table in the current method(A% only),there can be no unidentified peaks in
the method.
82
Display: UNIDENTIFIED PEAK FACTOR
The unidentified peak factor (UPF) is used to scale peaks which have not been listed in the Peak
Table and which therefore do not have individual response factors. A large factor will cause any
unidentified peaks to stand out in the report for easy detection,while a very small factor will
allow the area and retention time to appear in the report without influencing the results for the
identified peaks. This factor is not used in the Area Percent calculation.
Another application of the UPF is the case where a number of peaks for which individual
calibration standards are not available must be analyzed. If this group of peaks can be
represented by one typical compound for which a response factor is known, then application of
this factor to all members of the group may permit reasonable quantitation.
Display: SAMPLE ID
See HELP 19, display PRGM n SAMPLE ID:
INTEGRATION Displays (cont.)
Display: SUBTRACT BLANK BASELINE?
Entering [YES] allows the blank baseline currently stored in memory to be subtracted from
chromatograms run by this method.
When there is a rising baseline due to increasing column bleed with increasing temperature
during a temperature-programmed method, subtracting blank baseline may permit more accurate
quantitation, particularly of smaller peaks eluting during the later, steeper portions of the
chromatogram.
Since the stored baseline is not automatically deleted when the temperature program is altered, it
is important to re- equilibrate the system and collect a new blank baseline whenever the
parameters of a temperature program are modified.
This function should not be used to attempt to correct for a randomly wandering baseline
due to insufficient column equilibration time, contamination, etc. Instead, the cause of the
baseline instability should be corrected before attempting to run samples.
35. TIME EVENTS Displays
Display: ADD TIME EVENTS SECTION?
Press [YES] [ENTER] to add the TIME EVENTS section, which is used for controlling the
parameters the method will use to collect data for calculations. If you do not build this section(by
pressing[NO] [CENTER], the preset values of signal/noise ratio and initial peak width will be
used. Deleting either the TIME EVENTS or INTEGRATION section will delete both
sections from the method.
83
Display: PEAK REJECT VALUE
This value is the smallest height or area(see HELP 38, PEAK
MEASUREMENT PARAMETER) which will be included in the calculations and report. Peaks
smaller than the current peak reject value can be detected and stored in memory and included in a
recalculated report by lowering the peak reject value and pressing [SHIFT] [REPORT].
Display: SLGNAL TO NOISE RATIO
At the beginning of each manual run and each automation sequence,the data system measures the
baseline noise of the detector specified in that method. This noise value is multiplied by the signal
to noise ratio to obtain the integration sensitivity which will be used by the method. The larger the
signal to noise ratio, the larger a peak must be before it will be detected.
Values of 2 to 5 are commonly used for high sensitivity analyses,while values of 10 to 20 may be
used when integration of only larger peaks is desired, or when it is desirable to have the data
system detect baseline frequently during the run. The S/N ratio appears on the short report as
“NOISE”, under “MULTIPLIER.”
Display: TANGENT PEAK HEIGHT
When two or more adjacent peaks are not com1etely baseline resolved. The data system must
decide how to divide the area or height of the combined peaks so that the contribution of each
component to the total is accurately reflected in the report.
In general, if the earlier eluting of the fused peaks is much larger than the later eluting component,
it's reasonable to divide the area or height by drawing a tangent under the later peak and adding
any residual area to the area of the earlier peak. For fused peaks of similar size,division of the
area by dropping a perpendicular to baseline from the valley point between them may be more
reasonable. Tangent percent is used by the data system to determine which calculation to use.
TIME EVENTS Displays (cont.)
Display: TANGENT PEAK HEIGHT (cont.)
For example, if a tangent percent of 10 is entered, any later eluting peaks in a fused group that are
less than 10% as 1arge as the “mother” peak will have a tangent peak drawn under them and the
residual area added to the mother peak. If a fused peak is detected which is more than 10% as
large as the mother peak, a pependicular is dropped from the valley point between it and the
mother peak, and the later peak becomes the new mother peak for subsequent tangent calculations.
If a tangent percent of 0% is selected, all fused peaks will be calculated by perpendicular drop,
while a value of 100% will force calculation of all fused peaks as tangents.
Display; INITIAL PEAK WIDTH IN SEC
This entry is used to adjust the effective sampling rate of the data system to changing peak shapes
during a chromatogram. The data system calculates the width at half height of all detected peaks.
If no time-programmed peak width events have been entered in the method, the initial peak width
84
will be used to set the sampling rate until after a resolved peak with a half height width more than
50% greater than the current width parameter is detected,at which time the peak width parameter
will be automatically doubled. In the case of a group of fused peaks, the average peak width of
the group is used in deciding whether to update bunch.
Less commonly, the half height peak width may decrease during a run, in which case the
detection of a resolved peak with a width less than 75% of the current peak width parameter will
cause the system to halve the peak width parameter for subsequent data collection.. This
automatic updating of peak width parameter by the detected peaks is overridden whenever a
time-programmed peak width event is entered in the method.
Any change in the width, user-programmed or automatic, will be indicated in the run log and if
the user selects, as an annotation an the p1ot.
Display: CREATE TIME EVENTS TABLE?
Press [YES] [ENTER] if you wish to use the time events to change the way in which data is
collected and calculated during the run. Press [NO] [ENTER] if the initial values are suitable for
the entire run.
TIME EVENTS Displays (cont.)
Display: PRGM n EVENT
This entry selects the type of the next event to be entered in the Time events Table. Note: Once
an event type(WI,II,GR, SR, or YB) is entered, it cannot be changed unless this program
step is deleted (using the DELETE PROGRAM key) then rebuilt with the new event type.
WI event: Used to adjust the effective sampling rate and slope sensitivity of the system to match
the width at half height of the peaks in the chromatogram.
II event: Used to force the data system to designate event start and stop times as baseline points,
and prevent any collection of peak data between those times. Commonly used to reset baseline
after a detector range change or column switching valve upset.
GR event: Sums the response for all peaks with retention times between the start and stop time
of the event and assigns the total area to a peak with a retention time which is the average of
those-two times. The “group” peak is treated like any other peak in the calculations but is
identified by a separation code "GR” in the report.
SR event: Used to reject the area or height of the solvent or other extraneous peaks from the
calculated results of a run. Any peaks with retention times between the start and stop time of the
event are excluded from the calculations.
85
VB event: Designates every valley as baseline points integration purposes. VB can be used
when the chromatogram complexity prevents a data system from detecting baseline during a run,
but should be used with caution, since even a valley point near the apex of a major peak could be
defined as a baseline point, and a major peak could be almost completely lost from the
calculations.
Display:
PRGM n START TIME and PRGM n END TIME
The program start and end times define the time window during which the current time event
will be in effect. In the case of the peak width event, only the start time is used,the stop time
being replaced by the desired value of the parameter.
TIME EYENTS Displays (cont.)
Display: PRGM n PEAK WIDTH IN SEC
Desired value of peak width parameter from current time onward. By adding a times peak width
event, the automatic peak width updating done throughout the run is turned off. Insert enough
peak width events to keep peak width parameter about equal to the width at half height of the
peaks of interest in the chromatogram.
Display: ADD NEXT TIME EVENT?
Press [YES] [ENTER] to add other timed events or [NO] [ENTER] to exit to other method
sections.
36. PEAK TABLE Displays
Display: ADD PEAK TABLE SECTIQN?
You must add the PEAK TABLE section to do Internal Standard(IS),External Standard(ES), or
Normalized Percent (N%) calculations, or to identify peaks in any type of calculation.
If you want only an Area Percent (A%) report without identified peaks,no PEAS TABLE section
is required.
Display:
TABLE NUMBER OF STANDARD PK
The Internal Standard (IS) method of calculation requires that one peak in the chromatogram be
identified as the standard peak. This entry points to the peak number in the Peak Table which has
the retention time and other information pertaining to the standard peak:
Note that this is the number of the standard peak in the Peak Table only. Early eluting
unidentified peaks or identified peaks missing from the chromatogram may change the number of
the peak in the report, but this will not influence the identification of the standard peak, since it is
identified by comparison with its peak number listed in the Peak Table.
Display: TABLE NUMBER OF REL RETENTION PK
86
Identifies which peak in the Peak Table is to be used by the system in calculating relative
retention times. If peak is not identified in the chromatogram, the expected retention time listed
for it in the Peak Table will be used in the calculation. If the peak is detected,its actual retention
time is used. In either case, the unretained peak time will be subtracted from the retention times
of all peaks to correct for column dead volume. If the relative retention peak is identified as peak
0 in the method,the calculation will be skipped.
PEAK TABLE Displays (cont.)
Display: UNRETAINED PEAK TIME
The time it would take a totally unretained sample component to elute from the column under the
conditions of the method. This time can be determined by injecting air, methane, or some other
very weakly retained sample which will cause an observable detector response.
Display: RESPONSE FACTOR TOLERANCE-%
When a calibration mode run is specified, the data system calculates the response factor for each
identified peak and compares the calculated value with the value currently 1isted in the Peak
Table entry for that peak. If the calculated value is within the response factor tolerance
percentage o f the table value, the new factor is considered valid, otherwise the new value will be
considered out of tolerance and that fact will be noted in the error log at the end of the report.
In either case, the newly calculated value of the factor will appear in the report. Refer to HELP
number 19, PRGM n RUN MODE, for further information on automatic updating and averaging
of response factors.
Displays: REF PEAK WINDOW-MIN
REF PEAK WINDOW- %
NON-REF PEAK WINDOW-MIN
NON-REF PEAK WINDOW- %
The sum of these 2 parameters determines the time window within which a reference peak can be
identified. Using 2 parameters allows more flexibility in setting time window size for both early
and late eluting ref. peaks.
For example,a 2% window would represent a very reasonable ±0.2 minutes for a peak eluting at
10 minutes, but would provide only±0.01 minutes window (0.6 seconds) for a peak eluting at
+0.5 minutes. By adding 0.05 minutes through the REF PEAK WINDOW-MIN entry, the
window at 0.5 minutes can be widened to allow for some variation in injection timing without
unduly broadening the window at 10 minutes.
Note that the only difference between the REF PEAK WINDOW and the NON-REF PEAK
WINDOW entries is that the largest peak within the REF PEAK WINDOW is identified as the
reference peak,whereas the peak closest to the listed retention time in the NON-REF PEAK
WINDOW, regardless of size, is identified as the non-reference peak for that window. Thus it is
possible to make the windows for reference peaks somewhat wider to ensure that the reference
peaks are found, since only the largest peak in the window will be considered.
PEAK TABLE Displays (cont.)
Display: CREATE PEAK TABLE?
87
Press [YES] [ENTER] if you wish to identify peaks on the basis of retention time and quantitate
them by Internal Standard (IS), External Standard (ES), or Normalized Percent (N%) methods.
You may create a table of identified peaks for use with Area Percent (A%) calculation, but it is
not required.
Display: PEAK n EXPECTED TIME
This is the expected retention time of the component identified on this line of the Peak Table.
Only peaks detected within the appropriate reference or non-reference windows around the listed
retention time will be considered. For identification as the listed peak,regardless of their order or
number in the report.
Display: PEAK n PEAK NAME
Up to 8 alphanumeric characters may be entered to be used as the name of the peak in the report.
Display: PEAK n REL RESP FACTOR
This factor represents the relative response of the detector to the compound identified in this line
of the Peak Table, typically based on injection of known amounts of the components of interest in
a calibration mode run, although relative response factors can be calculated and entered by hand
if desired.
Entering a value of 0 for the relative response factor of one or more peaks in the peak table causes
the data system to enter the response factor calculated for those peaks in a calibration run table
without testing for factor tolerance. This is recommended at the beginning of each automated
sequence to ensure that new samples are not calculated using old leftover calibration factors. If
this factor is 0.0 in an analysis run, the result will be 0.0. If all factors are 0 in an analysis run,
that is an error which will cause the calculation to default to A%.
PEAK TABLE Displays (cont.)
Display: PEAK n CALIB AMOUNT
This entry is the amount of the current component which is contained in the calibration sample
which will be used to calibrate this method. The amount should be expressed in the units you
wish to appear in the report; i.e. enter amounts in ppm if you wish the report to be interpreted as
ppm.
Display: PEAK n REFERENCE PEAK?
Reference peaks are components of your sample which are always present,are reasonable
well-resolved from adjacent peaks,and are the largest peaks in their immediate vicinity of the
chromatogram. Peaks which satisfy these criteria are readily identifiable by the data system, and
88
can therefore be used as milestones or internal time signals by the data system as it attempts to
correctly identify the peaks in your chromatogram.
The IBDH system does this by first identifying the reference peaks in the chromatogram and
noting how their actual retention times differ from their listed retention times. It then uses these
deviations to adjust the expected retention times of the intersperses non-reference peaks. Thus, if
the carrier gas flow rate has decreased 2% since retention times were last determined, the
retention times of the reference peaks will have increased 2%, and the data system will use that
2% factor to increase the expected retention time of the non-reference peaks by the same factor,
helping to assure proper identification despite the flow rate change.
Display: ADD NEXT PEAK?
Press [YES] [ENTER] if you wish to add more peaks to the Peak Table.
89
NOTE
For ease in locating any display’s HELP number, refer to the
specific display (listed alphabetically )in this index and find its
HELP number listed after it.
Display
HELP Number
A
ADD AUXILIARY SECTION? NO
ADD DETECTOR B SECTION ?
ADD INUECTOR B SECTION? NO
ADD INTEGRATION SECTION ?
ADD NEXT AUXILIARY PROGRAM? NO
ADD NEXT ECD A/B PROGRAM? NO
ADD NEXT FID A/B PROGRAM ? NO
ADD NEXT FPD A/B PROGRAM ? NO
ADD NEXT HALL A/B PROGRAM? NO
ADD NEXT PEAK?
ADD NEXT PID A/B PROGRAM? NO
ADD NEXT PLOTTER PROGRAM? NO
ADD NEXT SEQTABLE PROGRAM? NO
ADD NEXT TCD A/B PROGRAM ? NO
ADD NEXT TIME EVENT ?
ADD NEXT TSD. A/B BEAD POWER ON ? NO
ADD NEXT INJECTOR PROGRAM ? NO
ADD NEXT INJECTOR B PROGRAM? NO
ADD NEXT RELAY PRGM? NO
ADD PEAK TABLE SECTION ?
ADD PLOTTER SECTION ? NO
ADD RELAY SECTION ? NO
ADD TIME EVENTS SECTION ?
AMOUNT STANDARD
A/S FAST INJ RATE IN UL/SEC (8035 A /S only)
A/S SLOW INJ RATE IN UL/SEC
AUTOMATION ACTIVE
AUX aaa0 SET ss (EQUILIBRATING)
AUX aaa0 SET sss HOLD xxx .xx
AUX aaa0 SET sss or OFF
AUX aaa0 SET sss RATE rrrr
AUX aaa0 SET sss STABILIZE
AUXILIARY OFF IN GC CONFIGURE
90
13
15
13
34
13
15 ECD
15 FID
15 FPD
15 HALL
36
15 FID/FPD
17
22
15 TCD
35
15 TSD
13
13
19
36
17
19
35
34
28
28
3
14
14
14
14
14
2, 3, 13
AUXILIARY OVEN ON ? YES
AUXILIARY SECTION? NO
AUXILIARY TEMP LIMIT
27
2, 6
25
C
COL aaa0 INJ aaa0 DET aaa0
COL aaa0 INJ aaa0 AUX aaa0 DET aaa0
COL aaa0 SET sss EQUILIBRATING
COL aaa0 SET sss HOLD xxx .xx
COL aaa0 SET sss or OFF
COL aaa0 SET sss RATE rr.r
COL aaa0 SET sss STABILIZE
COL aaa0 SET sss STANDBY
COL A /B VELO aa CM/SEC ss
COLUMN A /B CARRIER GAS
COLUMN A/B ID IN MICRONS
COLUMN A/B INSTALLED ?
COLUMN A /B LENGTH IN METERS
COLUMN IN CM /SEC VELOCITY A/B
COLUMN STANDBY TEMP ?
COLUMN TEMP LIMIT
COOLANT TIME OUT IN MIN INF
COOLANT TO COLUMN? NO
COOLANT TO INJECTOR? NO
COPY COMPLETE
CREATE PEAK TABLE ?
CREATE TIME EVENTS TABLE?
CURRENT LOCK CODE [ ][ ][ ][ ]
10
10
12
12
12
12
12
12
12
32
32
32
32
11
29
29
28
31
31
4
40
39
32
D
DELETE AUX SECTION ONLY? YES
DELETE DETECTOR A OR B?
DELETE INJECTOR B SECTION ONLY? YES
DESTRUCTIVE RAM TEST?
DET aaao SET sss or OFF
7
7
7
32
16
16 ECD,
16FID/PID
16 TCD, 16TSD
2, 3, 15
7
27
2,6,8
DET A/B OFF
DET OVEN A/B OFF IN GC CONFIGURE
DET OVEN A OR B OR DET A OR B ?
DETECTOR A ON ? YES
DETECTOR A OR B?
91
DETECTOR A OR B ? A
DETECTOR A/B OFF IN GC CONFIGURE
DETECTOR B ON? YES
DETECTOR A/B OVEN ON ? YES
DETECTOR A/B TEMP
DETECTOR TEMP LIMIT
DIVISOR
15
15
27
27
15
25
34
E
ECD A/B ATTEN aaaa RANGE xxx
ECD A/B AUTOZERO ON ? NO
ECD A/B BASELINE xxxxx.xx MV (A/Z)
ECD A/B INITIAL ATTEN
ECD A/B INITIAL RANGE
ENABLE COLUMN STANDBY TEMP ? NO
ENTER DATE - DAY MONTH xxxx
ENTER DATE - YEAR xx
ENTER LOCK CODE [ ][ ][ ][ ]
ENTER PROGRAM NO. TO DELETE --ENTER TIME OF DAY AS HHMM xxxx
16ECD
15ECD
16ECD
15ECD
15ECD
25
24
24
5
6
24
F
FAULT xx
FID A/B ATTEN aaaa RANGE xxx
FID A/B AUTOZERO ON ? NO
FID A/B BASELINE xxxxx.xx MV (A/Z)
FID A/B INITIAL ATTEN
FID A/B INITIAL RANGE
FIRST USER NUMBER?
FLOW A/B aaa SET xxx
FLOW A/B IN ML /MIN
FPD A/B ATTEN aaaa RANGE xxx
FPD A/B AUTOZERO ON ? NO
FPD A/B BASELINE xxxx:xx MV(AZ)
FPD A/B INITIAL ATTEN x
FPD A/ B INITIAL RANGE x
FPD A/PM TUBE xxx VOLTS
10, 32
16 FID
15 FID
16 FID
15 FID
15 FID
17
12
11
16 FPD
15 FPD
16 FPD
15 FPD
15 FPD
16 FPD
G
GC CONFIGURE TABLE COMPLETE
29
92
H
HALL A/B ATTEN aaaa VENT OPEN (CLOSED)
HALL A/B INITIAL ATTEN x
HALL A/B VENT CLOSED
HALL A/B VENT OPEN
HALL A/B VENT OPEN? YES
16 HALL
15 HALL
16 HALL
16 HALL
15 HALL
I
ILLEGAL ENTRY-PRESS PROMPT
ILLEGAL METHOD x
ILLEGAL SECTION
INCOMPLETE PROGRAM-DELETE IT? NO
INITIAL AUX HOLD TIME
INITIAL AUX TEMP
INITIAL COL HOLD TIME
INITIAL COLUMN TEMP
INITIAL INJ A HOLD TIME
INITIAL INJ B HOLD TIME
INITIAL INJ B TEMP
INITIAL INJECTOR A TEMP
INITIAL PEAK WIDTH IN SEC
INITIAL PLOT SPEED IN CM/MIN
INITIAL RELAYS xxxx
INITIAL RELAYS AT RUN END? YES
INJ A CLEARED/INJ B DELETED
INJ A - INJ B OR AUX?
INJ A/B aaao SET ss (EQUILIBRATING)
INJ A/B aaao SET sss HOLD xxx.xx
INJ A/B aaao SET sss RATE rrrr
INJ A/B aaao SET sss STABILIZE
INJ A/B aaao sss 100 or OFF
INJ B aaao DET B aaao AUX aaao
INJ B aaao SET ss(EQUILIBRATING)
INJ B aaao SET sss HOLD xxx.xx
INJ B aaao SET sss or OFF
INJ B aaao SET sss RATE rrrr
INJ B aaao SET sss STABILIZE
INJ B SECTION? NO
INJECT IN?
INJECTOR A/B OFF IN GC CONFIGURE
INJECTOR A/B OVEN ON? YES
INJECTOR A/B TEMP
30
10
2, 8, 31
30
13
13
11
11
13
13
13
13
35
17
19
36
7
2, 6, 7
14
14
14
10
14
14
14
14
14
2, 6
28
2, 3, 13
27
13
93
INJECTOR A/S TEMP LIMIT
INSTR STATUS - NO USER ENTRY
INSTRUMENT BUSY-CANNOT ENTER TEST
INSTRUMENT EVENT CODES? NO
25
10
32
17
K
KEY ECHO TEST?
32
L
LAST USER UNMBER?---LONG REPORT FORMAT?
17
34
M
METHOD COMPLETE - END TIME xxxxx
METHOD LOCKED (OR UNLOCKED)
METHOD RUNNING
METHOD x A/S SAMPLING
METHOD x INACTIVE-END TIME xxxxx
METHOD x WAITING FOR EXT DEVICE
METHOD x A/S WAITING xxxxx MIN
METHOD x COMPUTING
METHOU x MONITOR
METHOD x RUN xxxxx END xxxxx MIN
METHOD x STABILIZE xxxxx MIN
MULTIPLIER
21
4, 5, 6, 7, 8, 31
3, 4, 6, 7
10
10
10
10
10
10
10
10
34
N
NEW LOCK CODE xxxx
NEXT RUN NUMBER? nnnn
NO MORE TUNE ENTRIES
NON-REF PEAK WINDOW – MIN
NON-REF PEAK WINDOW - %
NO PLOTTER SECTION IN METHOD
NO RELAY SECTION IN METHOD
NOT IN AUTOMATION
NOT IN RUN
28
28
8
36
36
18
20
2
2, 8
O
OTHER CONFIGURATIONS?
28
94
P
PAPER OUT
PEAK n CALIB AMOUNT
PEAK n EXPECTED TIME
PEAK n PEAK NAME
PEAK n REFERENCE PEAK?
PEAK n REL RESP FACTOR
PEAK MEASUREMENT PARAMETER?
PERK REJECT VALUE
PID A/B ATTEN aaaa RANGE xxx
PID A/B AUTOZERO ON?NO
PID A/B BASELINE xxxxx.xx MY(A/Z)
PID A/B INITIAL ATTEN
PID A/B INITIAL RANGE
PLOT ddd A/B AT vv.v CM/MIN ZERO xxx
PLOT SIGNAL A OR B?
PLOTTER BUSY
POWER FAIL/WARM START OCCURRED
PRES A/B aaa VEL A?B as SPLITR A/B aaa
PRESSURE A/B aaa SET 10
PRESSURE A/B IN PSIG
PRGM 1 AUX HOLD TIME
PRGM 1 AUX RATE IN 0/MIN--PRGM 1 COL HOLD TIME
PRGM 1 COL RATE IN 0/ MIN
PRGM 1 ECD A/B AUTOZERO? NO
PRGM 1 ECD A/B ATTEN x
PRGM 1 ECD A/B RANGE x
PRGM 1 ECD A/B TIME IN MIN--PRGM I END TIME
PRGM 1 EVENT
PRGM 1 FID A/B ATTEN x
PRGM 1 FID A/B AUTOZERO? NO
PRGM I FID A/B RANGE x
PRGM 1 FID A/B TIME IN-MIN--PRGM 1 FINAL AUX TEMP--PRGM 1 FINAL INJ A TEMP--PRGM 1 FINAL INJ B? NO?
PRGM 1 FINAL COL TEMP
PRGM 1 FPD A/B ATTEN x
PRGM 1 FPD A/B AUTOZERO? NO
PRGM 1 FPD A/B RANGE x
PRGM 1 FPD A/B TIME IN MIN---
9
36
36
36
36
36
34
35
16 FID/PID
15 FID/PID
16 FID/PID
15 FID/PID
15 FID/PID
18
17
9
32
10
12
11
13
13
11
11
15 ECD
15 ECD
15 ECD
15 ECD
35
35
15 FID
15 FID
15 FID
15 FID
13
13
13
11
15 FPD
15 FPD
15 FPD
15 FPD
95
PRGM 1 HALL A/B ATTEN x
PRGM I HALL A/B TIME IN MIN--PRGM I HALL A/B PENT OPEN? NO
PRGM 1 HOLD TIME
PRGM I INJ B HOLD TIME
PRGM 1 INJ A RATE IN O/MIN--PRGM 1 INJ B RATE IN O/MIN--PRGM 1 MULTIPLIER
PRGM 1 NUMBER OF RUNS--PRGM 1 PEAK WIDTH. IN SEC
PRGM 1 PID A/B ATTEN X
PRGM 1 A/B AUTOZERO? NO
PRGM l PID A/B RANGE x
PRGM 1 PID A/B TIME IN MIN--PRGM 1 PLOT SIGNAL A OR B?
PRGM 1 PLOT SPEED IN CM/MIN
PRGM 1 PLOTTER TIME IN MIN-PRGM 1 RELAY TIME IN MIN--PRGM 1 RELAYS xxxx
PRGM 1 RUN nn INJ nn
PRGM 1 RUN METHOD
PRGM 1 SIGNAL TO DATA SYSTEM
PRGM 1 START TIME
PRGM 1 TCD A/B ATTEN x
PRGM 1 TCD A/B AUTOZERO?NO
PRGM 1 TCD A/B POLARITY POS?
PRGM 1 TCD A/B RANGE x
PRGM 1 TCD A/B TIME IN MIN--PRGM 1 TSD A/B ATTEN x
PRGM I TSD A/B AUTOZERO? NO
PRGM 1 TSD A/B BEAD POWER ON?NO
PRGM 1 TSD A/B TIME IN MIN--PRGM 1 TSD A/B RANGE x
PRINT REPORT AT RUN END? YES
PRINT RUN LOG AT RUN END? NO
PRINT USER NUMBER? NO
PRINTING - SELECT NEXT OPERATION
PROGRAM DELETED
PROGRAM NOT IN METHOD
PROGRAM NOT IN TABLE
PROGRAMS NOT BUILT
15 HALL
15 HALL
15 HALL
13
13
13
13
19
22
35
15 FID/PID
15 FID/PID
15 FID/PID
15 FID/PID
17
17
17
19
19
23
22
15
35
15 TCD
15 TCD
15 TCD
15 TCD
15 TCD
15 TSD
15 TSD
15 TSD
15 TSD
15 TSD
17
17
17
9
6
6
6
6
96
R
REF PEAK WINDOW - MIN
REF PEAK WINDOW - %
RELAY TEST?
RELAYS ON xxxx
REPORT UNIDENTIFIED PEAKS
RESPONSE FACTOR TOLERANCE - %
RESULT CALCULATION TYPE?
RESULT UNITS
RETURN TO TEST MENU? NO
RUN MODE?
RUNS OF TABLE? SINGLE (MULTI)
36
36
32
20
34
46
34
34
32
34
22
S
SAMPLE ID?
SECTION CLEARED
SECTION DELETED
SECTION NOT IN METHOD
SELECT METHOD OR TABLE
SELECT METHOD/SECTION OR TABLE
SELECT METHOD TO COPY FROM
SELECT METHOD TO COPY TO
SELECT SECTION
SELECT SECTION OR ENTER FOR ALL
SELECT SECTION OR TABLE
SELECT SECTION TO DELETE
SELECT SECTION TO TUNE
SEQ AUTOMATION SUSPENDED
SEQ TABLE COMPLETED
SFQ TABLE NOT ACTIVE
SET CHECKS FOR GC READY? NO
SET COLUMN PARAMETERS? NO
SET LOCK CODE? NO
SET TEMP LIMITS? NO
SET TIMES OR DATE? NO
SFPD A/B PM TUBE xxx VOLTS
SIGNAL A OR B TO DATA SYSTEM
SIGNAL TO NOISE RATIO
SIGNAL SWITCHING TO DS? NO
SOUND KEYSTROKES? YES
SPLIT RATIO A/B as SET ss
SPLIT RATIO A/B x TO
34
7
7
2, 6, 7
3, 5
1, 6, 7, 9
4
4
6
9
2
7
8
24
22
23
26
28
28
25
24
16 FPD
15
35
28
28
12
11
97
START A/S AFTER RUN START
STOP AUTOMATION AFTER ERROR
SUBTRACT BLANK BRSELINE?
19
22
34
T
TABLE ACTIVE
TABLE DELETD
TABLE LOCKED (OR UNLOCKED)
TABLE NOT ACTIVE
TABLE NOT BUILT
TABLE NUMBER OF REL RETENTION PK
TABLE NUMBER OF STANDARD PK
TABLE SUSPENDED
TANGENT PEAK HEIGHT
TCD A/B ATTEN aaaa RANGE xxx
TCD A/B AUTOZERO ON? NO
TCD A/B BASELINE xxxxx.xx MV(A/Z)
TCD A/B FIL TEMP sss CUR sss MA
TCD A/B FILAMENT TEMP OFF
TCD A/B INITIAL ATTEN x
TCD A/B INITIAL RANGE x
TCD A/B POLARITY NEGATIVE
TCD A/B POLARITY POSITIVE
TCD A/B POLARITY POSITIVE? YES
TCD CARRIER GAS HELIUM? YES
TEMP PROGRAM AUXILIARY? NO
TEMP PROGRAM COLUMN? NO
TEMP PROGRAM INJECTOR A? NO
TEMP PROGRAM INJECTOR B? NO
TEST OK
TEST SESSION COMPLETED
THERMAL STABILIZE TIME
TIME PROGRAM ECD A/B?
TIME PROGRAM FID A/B? NO
TIME PROGRAM FPD A/B? NO
TIME PROGRAM HALL A/B? NO
TIME PROGRAM PID A/B? NO
TIME PROGRAM PLOTTER? NO
TIME PROGRAM RELAYS? NO
TIME PROGRAM TCD A/B? NO
TIME PROGRAM TSD A/B? NO
TIME TICKS? NO
TSD A/B ATTEN aaaa RANGE xxx
3, 6
6, 7
5, 6, 7, 31
2
3, 6, 7
36
36
22
35
16 TCD
15 TCD
16 TCD
16 TCD
15 TCD
15 TCD
15 TCD
16 TCD
16 TCD
15 TCD
28
13
11
13
13
32
32
24
15ECD
15 FID
15 FPD
15 HALL
15 FID/PID
17
19
15 TCD
15 TSD
17
16 TSD
98
TSD A/B AUTOZERO ON? NO
TSD A/B BASELINE xxxxx.xx MV(A/Z}
TSD A/B BEAD CURRENT IN AMPS
TSD A/B BEAD POWER ON? NO
TSD A/B INITIAL ATTEN
TSD A/B INITIAL RANGE
TSD A/B s .sss AMP vv v VOLT BIAS
TURN HARDWARE ON-OFF? NO
TUNE FID A/B PRGM n
TUNE PLOTTER PRGM n
TUNE RELAY PRGM n
15 TSD
16 TSD
15 TSD
15 TSD
15 TSD
15 TSD
15 TSD
27
8
8
8
U
UNDER REMOTE CONTROL
UNIDENTIFIED PEAK FACTOR
UNRETAINED PEAK TIME
3
34
36
V
VENT TEST
34
W
WAIT IF FAULTS PRESENT? NO
WAIT IF PAPER OUT? YES
WAIT FUR AUX TEMP READY?
WAIT FOR DET A/B TEMP READY?
WAIT FOR EXT DEVICE READY? NO
WAIT FOR FLOW READY? YES
WAIT FOR INJ A/B TEMP READY?
WAIT FOR PRESSURE READY? YES
WAIT FOR SPLIT RATIO READY?
WAIT FOR VELOCITY READY?
26
26
26
26
26
26
26
26
26
26
Z
ZERO OFFSET IN PERCENT
17
99
DISPLAYS
DISPLAYS
LIST OF METHOD AND TABLE DISPLAYS AND THEIR PROMPTS
NOTE
Because of production modifications which sometimes occur prior to
shipment, these lists may not exactly reflect your instrument displays.
DISPLAY
PROMT
GC CONFIGURE Displays
SET TIMES OR DATE? NO
THERMAL STABILIZE TIME 2.00
ENTER TIME OF DAY AS HHMM xxxx
ENTER DATE-DAY MONTH 0503
ENTER DATE-YEAR xx
COOLANT TIME OUT IN MLN
INF
SET TEMP LIMITS? NO
COLUMN TEMP LIMIT 250
INUECTOR TEMP LIMIT 250
AUXILIARY TEMP LIMIT 52
DETECTOR TEMP LIMIT 300
COLUMN STANDBY TEMP? 50
ENABLE COLUMN STANDBY TEMP?
YES TO ENTER CHANGES
0.00 TO 650.00
24 HOUR CLOCK-4 DIGIT NUMBER
4 DIGIT NUMBER – DAY FIRST
2 DIGIT yy
0.00 TO 650.00 OR INF
NO
SET CHECKS FOR GC READY? NO
WAIT FOR INJ TEMP READY? YES
WAIT FOR AUX TEMP READY? NO
WAIT FOR DET TEMP READY? YES
WAIT FOR FLOW READY? YES
WAIT FOR PRESSURE READY? YES
WAIT IF PAPER OUT? YES
WAIT FOR EXT DEVICE READY? NO
WAIT IF FAULTS PRESENT? NO
INITIAL RELAYS AT RUN END? YES
YES TO ENTER CHANGES
50
TO 420
50
TO 420
50 TO 420
50
TO 420
20 TO lim
YES ENABLES STANDBY TEMP CONTROL
YES TO ENTER CHANGES
NO IGNORES
NO IGNORES
NO IGNORES
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NO RESETS RELAYS AT GC READY
SET LOCK CODE? NO
CURRENT LOCK CODE [ ][ ][ ][ ]
NEW LOCK CODE,xxxx
YES TO ENTER CHANGES
0 OR USER CODE
0 OR USER CODE
TURN HARDWARE ON-OFF? NO
DETECTOR A ON? YES
YES TO ENTER CHANGES
YES TURNS HARDWARE ON
100
DETECTOR B ON? YES
DETECTOR OVEN ON? YES
INJECTOR OVEN ON? YES
AUXILIARY OVEN ON? NO
COOLANT TO COLUMN? NO
COOLANT TO INJECTOR? NO
YES TURNS HARDWARE ON
YES TURNS HARDWARE ON
YES TURNS HARDWARE ON
YES TURNS HARDWARE ON
YES TURNS HARDWARE ON
YES TURNS HARDWARE ON
OTHER CONFIGURATIONS? NO
TCD CARRIER GAS HELIUM? YES
NEXT RUN NUMBER? nnnn
SOUND KEYSTROKES? YES
SIGNAL SWITCHING TO DS? NO
YES TO ENTER CHANGES
NO IF NITROGEN CARRIER GAS
1 TO 9999
NO TURNS OFF KEYBOARD BEEP
YES TO ENTER CHANGES
GC CONFIGURE TABLE COMPLETE
SELECT NEXT OPERATION
COLUMN Method Section
INITIAL COLUMN TEMP
50
-99 TO 52 or
-99 TO xxx
0.00 TO 650 OR INF or
0.00 TO rpt x OR INF
0 TO xxx
0 TO 60 PSIG
YES BUILDS PRGM 1
50 TO lim or
iii TO xxx
iii TO 50.0
0 TO xxx OR INF
YES BUILDS PRGM 2
INITIAL COL HOLD TIME 20.00
FLOCW A/B IN ML/MIN
PRESSURE A/B IN PSIG 0
TEMP PROGRAM COLUMN?
PRGM I FINAL COL TEMP
NO
PRGM 1 COL RATE IN O/MIN
PRGM 1 COL HOLD TIME
ADD NEST COLUMN PROGRAM?
(Up to 4 PRGMs may be built )
NO
COLUMN STATUS Displays
COL aaa0 SET sss or OFF
COL aaa0 SET sss HOLD xxx .xx
GOL aaa0 SET sss RATE rr.r
COL aaa0 SET sss STABILIZE
COL aaa0 SET sss COOLING
COL aaa0 SET sss STANDBY
FLOW A/B aaa []SET xxx
PRESSURE A/B aaa []SET xxx
INSTR STATUS – NO USER ENTRY
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INJECTOR Method Section
101
(Standard Injector)
INJECTOR TEMP 50
-99 TO lim
(On-Column Capillary Injector)
INITIAL INJECTOR TEMP 50
INITIAL INJ HOLD TIME 0.00
-99 TO xxx
0.00 TO 650 OR INF or
0.00 TO rpt OR INF
YES BUILDS PRGM 1
iii TO lim or
Iii TO xxx
iii TO 300 OR INF
TEMP PROGRAM INJECTOR? NO
PRGM 1 FINAL INJ TEMP --PRGM 1 INJ RATE IN 0/MIN --PRGM 1 HOLD TIME
ADD NEXT INJECTOR PROGRAM? NO
PRGM 2 FINAL INJ TEMP
ADD AUXILIARY SECTION? NO
INITIAL AUX TEMP 50
0 TO xxx or INF
YES BULIDS PRGM 2
iii TO lim0
YES ADDS AUXILIARY SECTION
-99 TO lim or
-99 TO xxx
INITIAL AUX HOLD TIME 0.00
0.00 TO 650 MIN OR INF or
0.00 TO rpt MIN OR INF
YES BULIDS PRGM 1
TEMP PROGRAM AUXILIARY?
PRGM 1 FINAL AUX TEMP---
iii TO lim or
Iii TO xxx
PRGM 1 AUX RATE IN O/MIN---
0.1 TO 300 or
xxx.x TO 300
0.00 TO rpt OR INF
YES BUILDS PRGM 2
PRGM 1 AUX HOLD TIME 0.00
ADD NEXT AUXILIARY PROGRAM? NO
INJECTOR STATUS Displays
INJ aaa0 SET sss or OFF
INJ aaa0 SET sss NOLD xxx.xx
INJ aaa0 SET sss RATE rr.r
INJ aaa0 SET sss STABILIZE xxx.xx
INJ aaa0 SET sss (COOLING)
AUX aaa0 SET sss or OFF
AUX aaa0 SET sss HOLD xxx.xx
AUX aaa0 SET sss RATE rrrr
AUX aaa0 SET sss STABILIZE xxx.xx
AUX aaa0 SET sss (COOLING)
INSTR STAUS – NO USER ENTRY
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INSTR STAUS – NO USER ENTRY:
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DETECTOR Method Section (A or B or both)
DETECTOR TEMP 50
20 TO lim
102
DETECTOR A OR B ? A
ddd x INITIAL ATTEN 8
ddd x INITIAL RANGE 9
SELECT A OR B
1 TO 1024 <POWERS OF 2> INF
As applies
TCD: 5 OR 0.5 OR 0.05
FID/TSD :8 OR 9 OR 10 OR
11 OR 12
ECD: 10 OR 1
FPD ; 8 OR 9 OR 10
SFP : 100 OR 10 OR 1
YES AUTOZEROS AT GC READY
ddd x AUTOZERO ON ? NO
TCD x FILAMENT TEMP OFF
50 TO 390 OR OFF or
50 TO 490 OR OFF
NO REVERSES SIGNAL POLARITY
NO TURNS BEAD CURRENT OFF
2.400 TO 3.800
1 TO 1024 <POWERS OF 2> OR INF:
SELECT A OR B
TCD x POLARITY POSITIVE ? YES
TSD x BEAD POWER ON ? NO
TSD x BEAD CURRENT IN AMPS 2.400
ddd x INITIAL ATTEN I
SIGNAL A OR B TO DATA SYSTEM
HALL x VENT OPEN? YES
TIME PROGRAM ddd x? NO
ADD XXXXX SECTION? NO
METHOD COMPLETE-END TIME xxxxx
PRGM 1 ddd x TIME IN MIN--PRGM I ddd x ATTEN x
PRGM 1 ddd x RANGE x
PRGM 1 ddd x AUTOZERO? NO
PRGM 1 TCD x POLARITY POS?
PRGM 1 TSD x BEAD POWER ON? NO
PRGMl DET x TIME IN MIN--PRGM 1 HALL x TIME IN MIN--PPGM I ddd x ATTEN I
PRGM 1 HALL x ATTEN 1
PPGM 1 HALL X VENT OPEN? NO
PPGM 1 SIGNAL TO DATA SYSTEM
ADD NEXT ddd x PROGRAM? NO
(UP TO 5 PRGMs available for each detector)
ADD ddd A/B SECTION? NO
YES TO OPEN VENT-NO TO CLOSE
YES BUILDS PRGM 1
NO MOVES TO NEXT SECTION
SELECT NEXT OPERATION
. 0.01 TO 650.00 OR TUNE
1 TO 1024 <POWER OF 2> INF
as for initial range conditions
YES AUTOZEROS AT GC READY
NO REVERSES SIGNAL POLARITY
NO TURNS BEAD SIGNAL POLARITY
0.00 TO 650.00 OR TUNE
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1 TO 1024<POWER OF 2> OR INF
1 TO 1024<POWER OF 2> OR INF
YES TO OPEN VENT-NO TO CLOSE
SELECT A OR B
YES BUILDS PRGM 2
NO MOVES TO NEXT SECTION
DETECTOR STATUS Displays
DET aaa0 SET sss or OFF
DETECTOR A OR B?
DET x OFF
TCD x FIL TEMP sss CUR sss MA
INSTR STATUS – NO USER ENTRY
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103
TCD x POLARITY POSITIVE (NEGATIVE)
FPD x PM TUBE xxx VOLTS
SFPD x PM TUBE xxx VOLTS
ddd x BASELINE xxxxx.xx MV(A/Z } :
ddd x ATTEN aaaa RANGE xxx
TSD x s.sss AMP vv.v VOLT BIAS
HALL x ATTEN aaaa VENT OPEN (CLOSED)
HALL x VENT OPEN(CLOSED)
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PLOTTER Method Section
ADD PLOTTER SECTION ? NO
NO MOVES TO NEXT SECTION
INITIAL PLOT SPEED IN CM/MIN 1.0
0.0 TO 30.0
TM
0 TO 100
ZERO OPPSET IN PERCENT 5 (15 IBDH )
PLOT SIGNAL A OR B ? A
SELECT A OR B
TM
TIME TICKS ? NO (YES IBDH )
YES PRINTS ON PLOT
TM
INSTRUMENT EVENT CODES ? NO (YES IBDH ) YES PRINTS ON PLOT
FIRST USER NUMBER?--1 TO 4 DIGITS
LAST USER NUMBER?--1 TO 4 DIGITS
PRINT USER NUMBER? NO
YES TO PRINT USER NUMBER
PRINT REPORT AT RUN END? YES
YES TO PRINT REPORT
PRINT RUN LOG AT RUN END? NO
YES PRINTS HISTORY OF RUN
TIME PROGRAM PLOTTER? NO
YES BUILDS PRGM 1
PRGM 1 PLOTTER TIME IN MIN ---
0.01 TO 650.00 OR TUNE or
xxxxx TO iiiii OR TUNE
0.0 TO 30.0
SELECT A OR B
YES BUILDS PRGM 2
PRGM 1 PLOT SPEED IN CM/MIN x.0
PRGM 1 PLOT SIGNAL A OR B? A
ADD NEXT PLOTTER PROGRAM? NO
PLOTTER STATUS Displays
PLOT ddd x AT vv.v C/M ZERO xxx
NO PLOTTER SECTION IN METHOD
INSTR STATUS – NO USER ENTRT
INSTR STATUS – NO USER ENTRT
Additional A/S Displays With IBDHTM Option
NUMBER INJ/CALIB VIAL 1
INJ PER ANALYSIS VIAL 1
SAMPLE TABLE ? NO
PRGM n A/S PACK NUMBER
PRGM n A/S VIAL NUMBER
PRGM n RUN MODE
1 TO 8
1 TO 8
YES BUILDS PRGM 1
1 TO 16
1 TO 15
1=ANALYSIS
2=CALIB
3=BLANK BASE
104
PRGM n SAMPLE ID
PRGM n AMOUNT STANDARD
PRGM n MULTIPLIER
PRGM n DIVISOR
ADD NEXT SAMPLE PROGRAM? NO
SAMPLE NAME
0.000001 TO 1000000
0.000001 TO 1000000
0.000001 TO 9999999
YES BUILDS PRGM n
NUMBER INJ/CALIB VIAL 1
INJ PER ANALYSIS VIAL 1
SAMPLE TABLE ? NO
PRGM n A/S PACK NUMBER
PRGM n A/S VIAL NUMBER
PRGM n RUN MODE
1 TO 8
1 TO 8
YES BUILDS PRGM 1
1 TO 16
1 TO 15
1=ANALYSIS
2=CALIB
3=BLANK BASE
SAMPLE NAME
0.000001 TO 1000000
0.000001 TO 1000000
0.000001 TO 9999999
YES BUILDS PRGM n
PRGM n SAMPLE ID
PRGM n AMOUNT STANDARD
PRGM n MULTIPLIER
PRGM n DIVISOR
ADD NEXT SAMPLE PROGRAM? NO
RELAY Method Section (Optional)
ADD RELAY SECTION? NO
INITIAL RELAYS xxxx
TIME PROGRAM RELAYS? NO
PRGM 1 RELAY TIME IN MIN---
NO MOVES TO NEXT SECTION
1234 FOR ON/-1-2-3-4 FOR OFF
YES BUILDS PRGM 1
0.01 TO 650 MIN OR TUNE or
0.01 TO xxxxx MIN OR TUNE
1234 FOR ON/-1-2-3-4 FOR OFF
YES BUILDS PRGM X
xxx TO 650 MIN OR TUNE
1234 FOR ON/-1-2-3-4 FOR OFF
xxx TO 650 MIN OR TUNE
SELECT NEXT OPERATION
PRGM 1 RELAYS
xxxx
ADD NEXT RELAY PRGM? NO
PRGM X RELAY TIME IN MIN
PRGM 2 RELAYS xxxxx
PRGM X RELAY TIME IN MIN
METHOD COM PLETE - END TIME xxxxx
RELAY STATUS Displays
RELAYS xxxx
NO RELAY SECTION IN METHOD
INSTR STATUS – NO USER ENTRY
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INTEGRATION Displays
ADD INTEGRATION SECTION? NO
RUN MODE?
1
NO MOVES TO NEXT SECTION
1=ANALYSIS
2=CALIB
105
3=BLANK BASE
1=AREA 2=HEIGHT 3=SQ RT
YES PRINTS LONG REPORT
1=A% 2=N% 3=IS 4=ES
0.000001 TO 9999999
0.000001 TO 9999999
0.000001 TO 1000000
8 ALPHANUMERIC CHARACTERS
NO REPORTS ONLY TOTAL AREA
0.000000 TO 999999.0
12 ALPHANUMERIC CHARACTERS
YES SUBTRACTS BASELINE
PEAK MEASUREMENT PARAMETER? 1
LONG REPORT FORMAT? NO
RESULT CALCULATION TYPE I
DIVISOR I.000000
AMOUNT STANDARD 1.00000
MULTIPLIER
1.00000
RESULT UNITS
REPORT UNIDENTIFIED PEAKS? YES
UNIDENTIFIED PEAK FACTOR 0.00000
SAMPLE ID
SUBTRACT BLANK BASELINE? NO
TIME EVENTS Displays
ADD TIME EVENTS SECTION?YES
PEAK REJECT VALUE 100
SIGNAL TO NOISE RATIO 5
TANGENT PEAK HEIGHT 10
INITIAL PEAK WIDTH IN SEC 2
CREATE TIME EVENTS TABLE? NO
PRGM n EVENT
PRGM n START TIME
PRGM n END TIME
PRGM n PEAK WIDTH IN SEC
ADD NEXT TIME EVENT? NO
NO MOVES TO NEXT SECTION
0 TO 10000000
2 TO 255
0 TO 100
.5 TO 256 IN POWERS OF 2
YES BUILDS PRGM 1
1=WI 2=II 3=SR 4=GR 5=VB
0.00 TO 650.0
0.00 TO 650.0
.5 TO 256 IN POWERS OF 2
YES BUILDS PRGM 2
PEAK TABLE .Displays
ADD PEAK TABLE SECTION?YES
TABLE NUMBER OF STANDARD PK 0
TABLE NUMBER OF REL RETEN PK
UNRETAINED PEAK TIME 0.000
RESPONSE FACTOR TOLERANCE-%5.0
REF PEAK WINDOW - MIN 0.10
REF PEAK WINDOW - %10.0
NON - REF PEAK WINDOW - MIN 0.10
NON-REF PEAK WINDOW - %5.0
NO MOVES TO NEXT SECTION
0 TO 99
0 TO 99
0.000 TO 650.000
0 TO 100.00
0.00 TO 650.00
0.0 TO 100.0
0.00 TO 650.00
0.0 TO 100.00
PEAK TABLE Displays (cont.)
CREATE PEAK TABLE? NO
PEAK n EXPECTED TIME
PEAK n PEAK NAME
YES BUILDS PEAK 1
0.000 TO 650.000
8 ALPHANUMERIC CHARACTERS
106
PEAK n REL RESP FACTOR
PEAK n CALIB AMOUNT
PEAK n REFERENCE PEAK?
ADD NEXT PEAK? NO
0.000000 TO 99999999
0.000001 TO 1000000
YES USES AS REFETENCE
YES BUILDS PEAK n
NO
BUILD/MODIFY Displays
SELECT METHOD/SECTION OR TABLE
METH 1-4 /SECTION/TABLE
ACTIVE LINE Displays
SELECT SECTION OR TABLE
DET A OR B?
AUXILIARY SECTION? NO
NOT IN RUN
NOT IN AUTOMATION
ILLEGAL SECTION
SECTION NOT IN METHOD
TABLE NOT ACTIVE
SELECT SECTION OR TABLE
SPECIFY A OR B
YES SELECTS AUX/NO SELECTS INJ
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
ACTIVATE Displays
SELECT METHOD OR TABLE
TABLE ACTIVATED
TABLE NOT BUILT
METHOD RUNNING
UNDER REMOTE CONTROL
METHOD ACTIVATED
AUTOMATION ACTIVE
TABLE ACTIVE
METH 1-4/TABLE
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
COPY Displays
SELECT METHOD TO COPY FROM
SELECT METHOD TO COPY TO
COPY COMPLETE
METHOD LOCKED
METHOD RUNNING
METH 1-4
METH 1-4
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
LOCK/UNLOCK Displays
ENTER LOCK CODE [][][][]
SELECT METHOD OR TABLE
METHOD LOCKED
0 OR USER CODE
METH 1-4/TABLE
SELECT NEXT OPERATION
107
METHOD UNLOCKED
TABLE LOCKED
TABLE UNLOCKED
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
PRINT and REPORT Displays
SELECT METHOD/SECTION OR TABLE
SELECT SECTION OR ENTER FOR ALL
PLOTTER BUSY
PAPER OUT
PRINTING - SELECT NEXT OPERATION
METH 1-4/SECTION/TABLE
METHOD SECTION OR ENTER
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
DELETE PROGRAM and DELETE SECTION/TABLE Displays
SELECT METHOD/SECTION OR TABLE
SELECT METHOD/SECTION OR TABLE
ENTER PROGRAM NO. TO DELETE--PROGRAM DELETED
SECTION DELETED
TABLE DELETED
METHOD LOCKED
TABLE LOCKED
DELETE DETECTOR A OR B? -DELETE AUX SECTION ONLY? YES
METHOD RUNNING
TABLE ACTIVE
SELECT SECTION
PROGRAMS NOT BUILT
SECTION CLEARED
INJ CLEARED/AUX DELETED
PROGRAM NOT IN TABLE
DETECTOR A OR B?
SECTION NOT IN METHOD
AUXILIARY SECTION? NO
PROGRAM NOT IN METHOD
SELECT SECTION TO DELETE
TABLE NOT BUILT
METH 1-4/SECTION/TABLE
METH/SECT OR TABLE TO DELETE
ENTER PROGRAM NUMBER
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SPECIFY A OR B
NO DELETES AUX AND CLEARS INJ
SELECT NEXT OPERATION
SELECT NEXT OPERATION
METHOD SECTION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SELECT NEXT OPERATION
SPECIFY A OR B
SELECT NEXT OPERATION
YES SELECTS AUX/NO SELECTS INJ
SELECT NEXT OPERATION
METHOD SECTION
SELECT NEXT OPERATION
SECTION CONTROL Displays
ADD XXXXXXX SECTION ? NO
NO MOVES TO NEXT SECTION
SEQUENCE TABLE Displays
RUNS OF TABLE?
SINGLE (MULTI)
SINGLE OR MULTI
108
STOP AUTOMATION AFTER ERROR 0
PRGM n RUN METHOD--PRGM n NUMBER OF RUNS--ADD NEXT SEQ TABLE PROGRAM? NO
SEQ TABLE COMPLETED
0 TO 99
1 TO 4
1 TO 99
YES BUILDS PRGM n
SELECT NEXT OPERATION
SEQUENCE TABLE STATUS Displays
SEQ TABLE NOT ACTIVE
PRGM X RUN nn
PRGM X RUN nn INJ nn
SEQUENCE AUTOMATION SUSPENDED
INSTR STATUS – NO USER ENTRY
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HELP Displays
SEE HELP NUMBER x (appropriate number displayed) (none)
Tune Mode Operation
SELECT SECTION TO TUNE
DETECTOR A OR B?
TUNE DET A/B PRGM n
TUNE PLOTTER PRGM n
TUNE RELAY PRGM n
NO TUNE ENTRIES
ILLEGAL SECTION
NOT IN RUN
METHOD LOCKED
PRESS METHOD SECTION TO TUNE
SPECIFY A OR B
PRESS ENTER TO TUNE
PRESS ENTER TO TUNE
PRESS ENTER TO TUNE
PRESS METHOD SECTION TO TUNE
PRESS METHOD SECTION TO TUNE
SELECT NEXT OPERATION
SELECT NEXT OPERATION
STATUS Section
METHOD X INACTIVE-END TIME xxxxx
METHOD X COMPUTING
METHOD X STABILIZE xxxxx MIN
METHOD X MONITOR
METHOD X WAITING FOR EXT DEVICE
METHOD X A/S WAITING xxxxx
METHOD X A/S SAMPLING
METHOD X RUN xxxxx END xxxxx MIN
NO XXXXXXX SECTION IN METHOD
COL aaa0 INJ aaa0 AUX aaa0 DET aaa0
ILLEGAL METHOD x
INSTR STATUS – NO USER ENTRY
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Fault Messages
109
See the Diagnostics/Troubleshooting section for Background Faults.
34 Series Operation
INCOMPLETE PROGRAM-DELETE IT?
ILLEGAL ENTRY 一 PRESS PROMPT
METHOD LOCKED
TABLE LOCKED
ADD XXXXXXX SECTION?
ILLEGAL METHOD x
TABLE SUSPENDED
NO
YES DELETES PROGRAM
prompt for expected entry
SELECT NEXT OPERATION
SELECT NEXT OPERATION
NO MOVES TO NEXT SECTION
INSTR STATUS – NO USER ENTRY
SELECT NEXT OPERATION
110
SPECIAL USES OF KEYS
1
INTRODUCTION
This section contains additional information about key functions. The descriptions contained in this
section may or may not apply to your specific application. For the basic functions of keys refer to
Figures 1 through 8 (1-9, 34 Series GC’s) in the Operation section
2
ADDITIONAL FUNCTIONS OF GC CONTROL KEYS
START

Pressing START
turns power to the optional accessory power outlet ON.

starts an active automation table or the autosampling Sequence. Pressing the inject switch
does NOT start automation. but starts the run for manual injection
has no effect if the GC is in RUN has started, or automation table is in progress,

RESET
Pressing RESET





stops the GC run,
stops automation and deactivates the active automation table,
turns the REMOTE CONTROL light OFF,
clears fault messages (blinking STATUS light),
restarts the coolant time-out timer






clears attenuation changes made via the ATTENUATION keys,
clears plotter "zero pen" action,
terminates the thermal stabilize timer and allows the
instrument to go immediately to READY,
returns column oven temperature to the initial conditions from
the standby temperature.
Certain end of run actions (as applicable) will occur when RESET is pressed while the instrument is in
RUN:
a . The RUN light will go OFF.
b. The GC resets to the initial conditions of the active method.
c. Tune mode is exited.
111
GC CONFIGURE
Updates to GC CONFIGURE are not entered in the Run Log.
(PLOTTER) START
Pressing (PLOTTER) START starts the plotter at the current conditions of the active method. If
printing or plotting is already in progress, pressing (PLOTTER) START has no affect.
ZERO PEN
Use ZERO PEN to bring a drifting baseline back on scale when autozero is undesirable. The pen
is returned to the zero offset value. The analog outputs are not affected
The zero pen offset remains unti1(a) RESET is pressed,or(b) the plot signal is switched to the
other detector,or (c) at end of run.
Zero pen offset is not cleared by autozero actions.
ATTENUATION Keys
All attenuation changes made during a GC run via the keyboard remain in effect until: (a)
RESET is pressed; (b) run end; (c) a time-programmed step resets attenuation; or (d) another method
is activated.
Changes made via the ATTENUATION keys do not update the method,but changes are included
in the INSTR EVENTS code annotations (specified、 in the PLOTTER method section) and in the
Run Log.
3
ADDITIONAL FUNCTIONS OF OPERATIONS KEYS ANO LIGHTS
Figure 3 in the Operation section describes the basic use of each OPERATIONS key and light. The
additional functions of specific OPERATIONS keys follow.
ACTIVE LINE (SHIFT, ACTIVE LINE)
ACTIVE LINE is used to display the controlling step within a method section or automation
Table during a run and is useful forquick program access to make real time updates during a run.
The GC Configure table is not considered as a table for this Display.
ACTIVATE
Bad or illegal methods can be activated,but the STATUS light will b1ink and the GC wi11
stay NOT READY. (Refer to pare. 5 in the Operation section,Using STATUS).
112
LOCK/UNLOCK
The lock/unlock feature protects the method via a user lock code entered in the GC Configure table.
A code of one to four digits is used to lock or unlock a method or table. The user lock code is
zero (0) if never changed in the GC Configure table.
PRINT
If a table is selected, printing begins immediately. If a method is selected, the user may
choose to print a single section or the entire method by responding to the displays. While printing, the
display will show that a print action is in progress. The display remains until the next operation is
selected.
If a print action is desired but the plotter is (1) busy or (2) out paper or runs out or paper during
printing, the display immediately shows the error condition. After paper is 1oaded, restart printing by
pressing PRINT.
REPORT (SHIFT , REPORT)
Use the REPORT key to print a report after a run. This is convenient to print a report for a
chromatogram even if PRINT REPORT AT RUN END was declined in the PLOTTER method
section。
The error log is part of the report. If a report is requested while a chromatogram. is being p1otted,
the display will show printer busy.
Refer to the Printer/Plotter section for Report/Error Log and Run Log formats. Refer to the IBDH
Operation section for augmented functions with the in-board data handling option.
TUNE ON
TUNE ON is used to activate the Tune rode.
Using Tune Mode
Tune mode allows you to enter a program time during a run if it was not entered in advance.
Programs in detector, printer/plotter, and relay sections can be tuned.
1
Specifying Tune Entries; Build the method section(s) as you normally would. For each
program to be tuned, press [TUNE/INF] and then [ENTER] when the display asks for the program
time. “TUNE” will appear in place of a time entry.
2
Activating Tune Mode: Before starting the run,press [TUNE ON] and the appropriate
SECTION CONTROL key( DETECTOR, PLOTTER, or RELAY). The GC is now in Tune mode.
113
Check that the program entry to be tuned first is displayed. If no tunable programs are present in the
selected section, “NO TUNE ENTRIES” will be displayed. All of the tunable programs in the section
may be viewed by scrolling with the
DISPLAY CONTROL keys.
3
Executing Tune Mode: Start the run and press [ENTER] when
The tunable program is to be activated.
The current run time is entered into the program in the method, the program is executed
immediately, and the next tunable program is displayed. “NO TUNE ENTRIES” will be displayed
when all tunable programs in the section are sorted into time order after tuning.
You may tune programs in more than one section during a run. However,since only one
section can be selected at a time, more than one run may be required to tune programs in different
sections whose desired times are similar.
DELETE SECTION/TABLE
The function of this key depends on the method section or table that is being deleted.
 If the method section is required (COLUMN, INJECTOR, DETECTOR) , pressing
DELETE SECTION/TABLE returns the section displays to their preset values and
deletes all PRGM lines.
 If a method has two detector sections and the DELETE DETECTOR SECTION action is
requested,you are asked which section(A or B)to delete as the ”non-required" section.
The remaining detector section then becomes a “required” section that can be "c1eared"
to presets by an additional press of DELETE SECTION/TABLE.
 Selective deletion of sections of a method is possible, e.g., the auxiliary section of the
injector section may be deleted.
 The GC Configure table cannot be deleted.
 If the GC is in RUN, active method sections or tables cannot be deleted.
4
ADDITIONAL FUNCTIONS OF TH# AUTOMATION CONTNOL KEYS
Movement within tables is the same as within method sections. When the required hardware is
not installed. the key will beep only, and no action will occur.
SEQUENCE TABLE
Sequence automation is halted when RESET is pressed or if an illegal method is encountered.
114
If SINGLE is chosen for RUNS OF TABLE, the table must be reactivated for each single pass of the
table.
(SEQUENCE TABLE) SUSPEND
If sequence automation has been suspended, it is resumed by pressing [START].
5
ENTRY KEYS
TUNE/INF
TUNE/INF is used instead of a time entry when building a table that you wish to tune.
paragraph 3, TUNE ON, for use of the Tune Mode.
Refer to
For infinity entries for time and attenuation, use TUNE/INF as Required. The appropriate response is
recognized by the instrument.
115
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