Biometra TRIO Manual
BIOMETRA
TRIO
Instruction Manual
Ver. 15/07
Please read these instructions carefully before using
this apparatus!
Biometra GmbH
Rudolf-Wissell-Str. 30
D-37079 Göttingen
Tel.: ++49 (0)551-50 68 6-0
Fax: ++49 (0)551-50 68 6-66
email: [email protected]
Internet: http://www.biometra.com
Service Department
Rudolf-Wissell-Strasse 14-16
D-37079 Göttingen
Tel.: ++49 (0)551-50 68 6-10 or -12
Fax: ++49 (0)551-50 68 6-11
email: [email protected]
This document describes the state at the time of publishing. It needs not necessarily agree
with future versions.
Subject to change!
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Instruction Manual TAdvanced TRIO 07/2015
1 Contents
1
Contents ......................................................................................................................................... 3
2
Introducing the Biometra TRIO Thermal Cycler ....................................................................... 7
3
4
5
6
2.1
Field of Applications ............................................................................................................. 7
2.2
General Description .............................................................................................................. 7
2.3
Special features .................................................................................................................... 8
2.3.1
Thermal Block ............................................................................................................... 8
2.3.2
High Performance Smart Lid ....................................................................................... 8
2.3.3
Display ............................................................................................................................ 8
2.3.4
User specific quick start function ................................................................................ 8
2.3.5
Easy programming........................................................................................................ 9
2.3.6
Temperature Optimisation Step (TOS)...................................................................... 9
2.3.7
GLP compliance ............................................................................................................ 9
2.3.8
Open system philosophy ........................................................................................... 10
2.4
Order numbers .................................................................................................................... 10
2.5
Technical specifications ..................................................................................................... 11
Legal Notes.................................................................................................................................. 13
3.1.1
Copyright ...................................................................................................................... 13
3.1.2
Liability .......................................................................................................................... 13
3.1.3
Meaning of instructions .............................................................................................. 13
Safety and Warning Notices ..................................................................................................... 14
4.1
Definition of Symbols ......................................................................................................... 14
4.2
General Safety Instructions ............................................................................................... 14
Installation .................................................................................................................................... 17
5.1
Content of delivery .............................................................................................................. 17
5.2
Unpack and Check ............................................................................................................. 17
5.3
Installation Conditions ........................................................................................................ 17
5.4
Operation Voltage ............................................................................................................... 18
Operating elements .................................................................................................................... 19
6.1
The Biometra TRIO front view .......................................................................................... 19
6.2
The Biometra TRIO rear view ........................................................................................... 19
6.3
High Performance Smart Lid (HPSL) .............................................................................. 20
6.4
Loading samples ................................................................................................................. 20
6.5
The Biometra TRIO Software............................................................................................ 21
6.5.1
Keyboard ...................................................................................................................... 21
6.5.2
Keypad ......................................................................................................................... 22
Instruction Manual TAdvanced TRIO 07/2015
3
7
6.5.3
Cursor keys .................................................................................................................. 22
6.5.4
Confirm or Cancel ....................................................................................................... 23
6.5.5
List of used commands and icons ............................................................................ 23
Operating ..................................................................................................................................... 28
7.1
Power On Self-Test ............................................................................................................ 28
7.2
Log in Screen ...................................................................................................................... 28
7.3
Log in .................................................................................................................................... 29
7.4
Home Screen....................................................................................................................... 31
7.5
Block status ......................................................................................................................... 31
7.6
Quick start function ............................................................................................................. 32
7.7
Incubation Mode ................................................................................................................. 35
8
Creating, Editing and Saving programs .................................................................................. 37
8.1
Programming modes .......................................................................................................... 37
8.2
Creating a new program/Use program template............................................................ 39
8.3
Set program name .............................................................................................................. 41
8.4
Set heated lid temperature ................................................................................................ 41
8.5
Set preheating mode .......................................................................................................... 41
8.6
Edit parameter or step ....................................................................................................... 42
8.6.1
Set cycles ..................................................................................................................... 43
8.6.2
Set temperature increment ........................................................................................ 44
8.6.3
Set time increment ...................................................................................................... 44
8.6.4
Adjust ramp rate .......................................................................................................... 45
8.6.5
Set Temperature Optimisation Step (TOS)............................................................. 45
8.7
Insert step ............................................................................................................................ 46
8.8
Delete step ........................................................................................................................... 46
8.9
Save Program ..................................................................................................................... 47
9
Starting, Copying and Deleting programs ............................................................................... 49
9.1
Start program....................................................................................................................... 49
9.2
Copy program...................................................................................................................... 51
9.3
Copy all programs............................................................................................................... 52
9.4
Delete program ................................................................................................................... 53
9.5
Delete all programs ............................................................................................................ 54
10
10.1
Display during run ............................................................................................................... 55
10.2
Pause Program ................................................................................................................... 56
10.3
Continue Program............................................................................................................... 57
10.4
Skip Step .............................................................................................................................. 57
10.5
Stop Program ...................................................................................................................... 57
11
4
Running, Pausing, Continuing and Stopping programs.................................................... 55
Protocol Wizard ....................................................................................................................... 59
Instruction Manual TAdvanced TRIO 07/2015
11.1
Select Polymerase .............................................................................................................. 59
11.2
Enter New Polymerase or Edit Polymerase ................................................................... 60
11.3
Delete Polymerase ............................................................................................................. 62
To delete a polymerase press ...................................................................................................... 62
11.4
Protocol Wizard parameter screen .................................................................................. 62
11.5
Primer Annealing Temperature Calculator ..................................................................... 64
12
Tools ......................................................................................................................................... 68
12.1
Settings................................................................................................................................. 68
12.1.1
Set Date and Time ...................................................................................................... 69
12.1.2
Automatic user logout ................................................................................................ 70
12.1.3
Configure Beeper ........................................................................................................ 71
12.1.4
Network ........................................................................................................................ 72
12.1.4.1
Network settings.................................................................................................. 72
12.1.4.2
Network user........................................................................................................ 73
12.1.5
Display brightness ...................................................................................................... 73
12.1.6
Touch screen calibration............................................................................................ 74
12.1.7
Factory settings ........................................................................................................... 75
12.2
User Management .............................................................................................................. 76
12.2.1
Create user .................................................................................................................. 78
12.2.2
Edit user ....................................................................................................................... 79
12.2.3
Delete user................................................................................................................... 82
12.3
Documentation .................................................................................................................... 82
12.3.1
Run-Logfile................................................................................................................... 83
12.3.2
Power-On-Logfile ........................................................................................................ 86
12.3.3
Extended Self-Test-Logfile ........................................................................................ 86
12.3.4
Error-Logfile ................................................................................................................. 87
12.4
Extended Self-Test ............................................................................................................. 88
12.5
Service Info File (SINF) ..................................................................................................... 90
12.6
Backup.................................................................................................................................. 91
12.7
Cycler Info ............................................................................................................................ 92
12.8
Contact ................................................................................................................................. 93
13
Adaptation of programs ......................................................................................................... 94
14
Short Manual ........................................................................................................................... 96
15
Troubleshooting .................................................................................................................... 101
15.1
Forgotten password .......................................................................................................... 101
15.2
Slow heating and cooling................................................................................................. 101
15.3
Auto restart ........................................................................................................................ 101
15.4
Auto restart due to unrecognized power failure ........................................................... 101
Instruction Manual TAdvanced TRIO 07/2015
5
15.5
Adaptation of protocols from other cyclers ................................................................... 101
15.6
Releasing wheel in case of blocked lid.......................................................................... 101
16
Maintenance and Repair ..................................................................................................... 103
16.1
Factory calibration ............................................................................................................ 103
16.2
Cleaning and Maintenance ............................................................................................. 103
16.3
Servicing and Repair ........................................................................................................ 103
16.4
Firmware update ............................................................................................................... 103
16.5
Replacement of spare parts ............................................................................................ 103
17
Service .................................................................................................................................... 104
17.1
Instructions for return shipment ...................................................................................... 104
17.2
Packing of the Biometra TRIO Thermal Cycler ............................................................ 104
18
Equipment Decontamination Certificate ............................................................................ 106
19
Note for the disposal of electric / electronic waste. ......................................................... 108
20
Declaration of Conformity .................................................................................................... 109
21
Warranty ................................................................................................................................. 110
22
Subject Index ......................................................................................................................... 111
6
Instruction Manual TAdvanced TRIO 07/2015
2
Introducing the Biometra TRIO Thermal Cycler
2.1 Field of Applications
Developed in 1983 by Kary Mullis PCR (Polymerase Chain Reaction) is now a common and
often indispensable technique used in medical and biological research labs for a variety of
applications. The Biometra TRIO is an end-point Thermal Cycler and intended to amplify
nucleic acids by repeated cycles of heating and cooling using DNA-Polymerases in a PCR
reaction. The development of the instrument had the goal to combine latest technology with
stylish design and user-friendly software. The user interface consists of a touchscreen with a
graphical or tabular display that shows the time, status, and temperature profile for each run.
A touchscreen keypad allows you to enter information into fields on the screen. The Biometra
TRIO can run three independent PCR reactions simultaneously and is especially helpful
when versatile samples are to be incubated using different PCR programs.
2.2 General Description
There are three models of the Biometra TRIO Thermal Cycler:



Biometra TRIO 48 for 0.2 ml tubes, plates or strips
Biometra TRIO 30 for 0.5 ml tubes
Biometra TRIO combi for 0.2 ml tubes, plates or strips or 0.5 ml tubes
The instrument is controlled by a 7’’ touchscreen display and easy to use interface. PCR
protocols and Run-Logfiles can be saved to a connected USB drive. By using the USB
functions PCR protocols can be exchanged easily between instruments and Run-Logfiles
can be stored for documentation reasons on PC.
Figure 1 The Biometra TRIO Thermal Cycler
The Biometra TRIO Thermal Cycler features an automatic restart. If a power failure occurs
during the run the instrument will continue the run when the power returns. In case of longterm power failure (longer than 30 minutes) the instrument keeps the sample block at 4°C
Instruction Manual TAdvanced TRIO 07/2015
7
(freeze step) and the user can decide to repeat the run with the same samples or to discard
them.
This instruction manual provides information on how to use all models of the Biometra TRIO
Thermal Cycler most effectively.
2.3 Special features
2.3.1
Thermal Block
The Biometra TRIO Thermal Cycler is available with different block formats. The 48 well
version can be used with standard 0.2 ml tubes, strips or plates. With 30 well blocks the
Biometra TRIO can be used with 0.5 ml tubes and the combi block version offers the
flexibility to incubate 0.2 ml tubes, strips or plates or 0.5 ml tubes. With with a sample
capacity of up to 144 samples in parallel the Biometra TRIO 48 can also be used for high
throughput applications.
By the rubber sealing at the lid an encapsulated space is formed when the lid is closed. The
closed room serves to improve the temperature uniformity of the sample block and avoids
formation of condensed water at the final PCR cooling step. Furthermore all blocks are
perfectly sealed to prevent condensed water from penetrating the Peltier elements
underneath the sample block and other parts of the electronics. By the perfect sealing the
Peltier elements are protected and the instruments life time is expanded.
2.3.2
High Performance Smart Lid
The Biometra TRIO heated lid has been optimized to heat twice as fast as with older models
and thus helps to shorten protocol run times. Moreover the heated lid serves for two
additional essential functions: It prevents the formation of condensed water at the reaction
tube portion located above the block surface level and it ensures reliable contact between
samples and thermal block by applying constant pressure. By the integrated clutch
mechanism the pressure applied to the reaction tubes is always same regardless of the
plastic ware height. The combination of the heated lid shape and the reliable contact
pressure ensure an even temperature distribution between samples thus significantly
improves the temperature uniformity.
With one press on the front button, the lid gently swings open and arrests in its end position.
By this mechanism the lid cannot fall down and the heated lid is out of the radius of action
when the user is inserting tubes or plates. Thus the risk of injury by violent pressure or the
risk of burn by hot surfaces is prevented.
2.3.3
Display
The Biometra TRIO Thermal Cycler features a 7`` state of the art color touchscreen user
interface. The display is built-in at a flat angle to ensure reflection-free viewing and
ergonomic programming. For programming PCR protocols the software incorporates
Biometras proven spreadsheet philosophy and alternatively offers graphical programming.
One touch leads from the spreadsheet to the alternative graphical programming mode. This
makes the creation of new or editing existing programs fast and easy.
2.3.4
User specific quick start function
Many other thermal cyclers offer a so called “latest program list”. In the latest program list a
certain number of PCR programs is collected and offered for quick start. The list is typically
ordered only by date and the user has to search for the desired program. In contrast the
Biometra TRIO software for each sample block creates a user specific list and only the latest
programs started by the currently logged-in user are offered for quick start. Even a lab
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Instruction Manual TAdvanced TRIO 07/2015
member does not use the instrument for a longer time the information on the latest programs
started by this user will not get lost.
For maximum convenience the Biometra TRIO software features additionally features the
program preview. Before start of a program the protocol steps are summarized in a clearly
arranged table by the program preview tool. The program preview therefore provides a
comprehensive overview on the protocol structure without the need to access programming
screens. The program preview is also available before quick start of programs.
2.3.5
Easy programming
Creating new PCR programs takes a lot of time if for each single step all parameters have to
be set manually. The Biometra TRIO offers two options for easy protocol creation:
1. Pre-installed program templates
2. Protocol wizard
The Biometra TRIO software offers several pre-installed program templates for different
applications. The program templates provide a general protocol structure and can be easily
adapted for the current experiment.
The protocol wizard offers the comfort to create specific PCR programs by just a few inputs.
The protocol wizard is based on program templates for specific polymerases. For up to eight
different polymerases program templates can be saved in the Biometra TRIO software and
just the annealing temperature, cycle number and product length has to be filled in to create
a specific PCR program. Optionally two-step or three-step PCR programs can be created by
the software. Furthermore the protocol wizard includes a primer annealing temperature
calculator and the calculated Ta value can be used for protocol creation.
2.3.6
Temperature Optimisation Step (TOS)
Finding the best primer annealing temperature is crucial for the specificity and efficiency of
PCR reactions. Often optimization of experiments is conducted only limited leading to
unspecific byproducts or reduced PCR sensitivity. By using the Biometra TRIO Temperature
Optimisation Step (TOS) function new primer pairs with unknown annealing temperatures
can be tested quickly and optimized in very short time.
The Temperature Optimisation Step (TOS) function makes use of the three sample blocks
and provides three different temperatures for a selected program step. Just set the
temperature for sample block 2 and define the temperature difference (temperature
increment) for block 1 and 3. Since the Biometra TRIO samples blocks work independent of
each other the maximum programmable temperature difference between the sample blocks
is at ±47.5°C.
2.3.7
GLP compliance
The software can manage up to 30 user accounts. By default three different user-levels are
available: Administrator, user with generic rights and user with limited rights. However, in a
comfortable menu the administrator can configure the settings for each user individually by
activating or inactivating single rights. By the user administration tool in combination with the
password protection of user accounts access to the instrument can be limited to authorized
persons and unwanted modifications to the system settings and PCR protocols prevented.
With each system start the Biometra TRIO performs an initial self-test and additionally the
software offers an extended self-test that can be initiated by the user. The results of the
extended self-test becomes are summarized in a protocol and are stored by the instrument.
Additionally to the self-test functions the instruments creates and stores run-logfiles for each
single run. For long-term storage run-logfiles and extended self-test protocols can be
exported as proprietary file format.
Instruction Manual TAdvanced TRIO 07/2015
9
2.3.8
Open system philosophy
The Biometra TRIO is an open system regarding consumables and reagents. Depending
upon the sample block the system can be used with single tubes, strips or plates. PCR plates
can be standard profile or low profile and they can be skirted, semi-skirted or non-skirted.
The TAdvance can handle all kind of plates and the plastic ware be sealed with domed caps,
flat caps, sealing foil or other technologies. Regardless of the used sealing method thanks to
the HPSL technology (see chapter 2.3.2) always the same pressure is applied to the
consumables serving for absolutely reproducible conditions.
The Biometra TRIO Thermal Cycler is not limited to reagents from a specific vendor and can
be used with any kind of polymerase. Regardless if isothermal-, hot start- or non-hot start
polymerases are used, the Biometra TRIO will produce reliable results. E.g. by programming
a pause step for the initial denaturation the system can be used for manual hot start
applications. After inserting samples into the hot block the PCR protocol can be started just
by pressing “continue”.
2.4 Order numbers
Model
Block type
Order No.
Biometra TRIO 30
Aluminum
846-x-070-720
Biometra TRIO 48
Biometra TRIO combi
Aluminum
Aluminum
846-x-070-723
846-x-070-724
Abbrevations:
x = 2 for 230V EN/DE, 4 for 115V EN, 5 for 100V EN
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Instruction Manual TAdvanced TRIO 07/2015
2.5 Technical specifications
Order Number
846-x-070-720
846-x-070-723
846-x-070-724
Name
Biometra TRIO 30
Biometra TRIO 48
Biometra TRIO combi
3 blocks for 30 x
0.5ml tubes each
Capacity
3 blocks for 48 x
3 combi blocks for 18
**
0.2ml tubes
x 0.5ml tubes
or 48 well microplates or 48 x 0.2ml tubes,
or 48 well
or 6 x strips of 8 each
microplates
or 6 x strips of 8
Block
Aluminum
Aluminum
Aluminum
Block coating
Special Alloy
Special Alloy
Special Alloy
Max. Heating Rate*
4.0 °C/sec
5.0 °C/sec
3.0 °C/sec
Max. Cooling Rate*
3.6 °C/sec
4.2 °C/sec
2.7 °C/sec
Avg. Heating Rate*
3.6 °C/sec
4.5 °C/sec
2.7 °C/sec
Avg. Cooling Rate*
3.2 °C/sec
3.8 °C/sec
2.4 °C/sec
Multiblock tool
Temperature Uniformity
Temperature Optimisation Step (TOS)
95°C
+/- 0.60 °C after 15 s
70°C
+/- 0.30 °C after 15 s
55°C
+/- 0.20 °C after 15 s
Temperature Range
3 °C to 99 °C
Control Accuracy
+/- 0.1 °C
Software
User specific quick start of the last five programs, program preview prior to
start, toggle between spreadsheet and graphical programming mode,
Temperature Optimisation Step (TOS), Service Info Files (SINF), Extended
Self-Test (EST), adjustable ramp rates, PC control via Ethernet,
comprehensive user administration tool, protocol wizard, annealing
temperature calculator
Program memory
Total capacity of 350 programs in up to 30 user directories
Display
7‘‘ Color Touchscreen
Auto restart after power failure
Yes
High Performance Smart Lid
(HPSL) Technology
Yes
Lid Temperature Range
30 °C to 110 °C
Power consumption
1000 Watt
Power Supply
100, 115, 230 Volt, 50-60 Hz
Noise emission
Very low
Interfaces
1
USB A, Ethernet
Working conditions
15 °C to 35 °C, 70 % air humidity, max 2000 m NN
Dimensions (WxDxH)
30 cm x 41 cm x 25 cm
Instruction Manual TAdvanced TRIO 07/2015
11
Weight
17.3 kg
* Measured inside the block
**
1
Capacity increases to 35 x 0.5 ml tubes by use of small cap tubes
The network cable must be at least performance class Cat 5e and the cable configuration has to be STP
x = 2 for 230V EN/DE, 4 for 115V EN, 5 for 100V EN
12
Instruction Manual TAdvanced TRIO 07/2015
3
3.1.1
Legal Notes
Copyright
All rights reserved. It is not allowed to copy and publish the manual or parts of it in any form
as copies, micro film or other methods without a written authorization from Biometra.
Biometra is pointing out that applied company and brand names are usually protected
trademarks.
3.1.2
Liability
Biometra is not liable for damages and injuries caused by use not considering these
operating instructions in parts or completely.
3.1.3
Meaning of instructions
Biometra recommends that you first read these instructions carefully. This operation
instruction is part of the product and should be kept over the full life-time of the instrument. It
should also be forwarded to subsequent owners and users. Make sure that additions and
updates are inserted into the operation instructions.
Instruction Manual TAdvanced TRIO 07/2015
13
4
Safety and Warning Notices
4.1 Definition of Symbols
Symbol
Definition
Caution! Refer to instruction manual!
Danger! High voltage!
Fragile!
Danger! Hot surface!
4.2 General Safety Instructions
Please read this manual carefully before starting operation of the Biometra TRIO Thermal
Cycler. The Biometra TRIO Thermal Cycler is intended for sample incubation at varying
temperatures.
General safety precautions for laboratory work must be observed when working with the
instrument.
The Biometra TRIO Thermal Cycler does not produce a sound power level hazardous for the
user.
The sample block and the heated lid will reach high temperatures
during operation. Both sample block and heated lid can burn you. Do
not touch the sample block or heated lid.
Rapid heating of the sample block can cause liquids to boil
explosively. Always wear safety goggles during operation. Close the
lid before starting a program.
Do not heat samples without having the lid locked securely.
Be aware that samples are reaching high temperatures. Do not touch
or open hot tubes or microplates because hot liquid may quickly spill
out.
Use only suited plastic ware in the Biometra TRIO Thermal Cycler.
Tubes and plates must show good fit when placed in the sample
block. Only use tubes that are suited for high temperatures (tight
lids).
The Biometra TRIO Thermal Cycler contains no user serviceable
parts. Do not open the housing instrument. Service and repair may
only be carried out by the Biometra Service department or otherwise
qualified technical personal.
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Instruction Manual TAdvanced TRIO 07/2015
Do not use the instrument when damages of the housing, block,
cable or other parts are visible.
Prior to connecting the unit to the power source, please ensure that
the voltage selector at the bottom of the instrument is set to the
required voltage (see chapter 5.4).
Make sure that the main supply voltage is in accordance with the
label above the power connection.
Unplug the power cable before you open the Biometra TRIO Thermal
Cycler. Danger of electric shock!
Make sure that the appliance connector and the plug of the supply
cord are accessible, so you can separate the instrument from the
mains.
Connect the Biometra TRIO Thermal Cycler to a grounded socket.
When only few samples are put in the block place additional tubes in
the four corner positions. This is to evenly distribute the lid pressure
and prevents single tubes from excessive pressure. Use of few tubes
may result in damage of the tubes by excessive pressure.
Appropriate safety regulations must be observed when working with
infectious, pathogenic or radioactive material. Ask the responsible
local safety inspector for details.
The Biometra TRIO Thermal Cycler must not be used with explosive,
flammable or volatile liquids.
Do not place fingers between lid and housing when opening or
closing the lid.
Before opening of the lid, release lid pressure (see section 6.3)
It is not necessary to apply oil into the opening of the block in order
to improve the heat transfer between the block and the sample
tubes.
If you still decide to use oil, do not use silicon oil. Mineral oil may be
used.
Ensure that both the rear and bottom ventilation slits not clogged by
dust or other material. Danger of overheating!
Let equilibrate the Biometra TRIO Thermal Cycler to room
temperature before starting operation.
There must be sufficient distance between the ventilation slots on the
rear side of the Thermal Cycler and a wall or another instrument (min
10 cm). Danger of overheating!
This instrument is designed and certified to meet EN 61010-1 safety
standards. It should not be modified or altered in any way. Alteration
of this instrument will void the warranty, void the EN61010-1
certification, and create a potential safety hazard.
Instruction Manual TAdvanced TRIO 07/2015
15
Place the Biometra TRIO Thermal Cycler on a stable, non-flammable
surface in a dry, safe environment. For details see working
conditions in table “Technical specifications” (see chapter 2.4).
Do not use alcohol (e.g. methanol, ethanol), organic solvents or
abrasives to clean the instrument.
For transport always use the original Biometra box.
16
Instruction Manual TAdvanced TRIO 07/2015
5
Installation
5.1 Content of delivery
1)
2)
3)
4)
Thermal Cycler
Mains connector
Manual
Short Manual
Please keep the original packaging material for return shipment in case of servicing. The
Biometra TRIO Thermal Cycler shipping box provides a specially developed system for
contact-free transport of this electronic device.
5.2 Unpack and Check
Unpack and carefully examine the instrument. Report any damage to Biometra.
Do not attempt to operate this device if physical damage is present!
Please keep the original packing material for return shipment in case of service issues.
5.3 Installation Conditions

Place the Biometra TRIO Thermal Cycler on a stable surface in a dry, safe
environment. For details see working conditions in table “Technical specifications”
(see chapter 2.4).

Let equilibrate the Biometra TRIO Thermal Cycler to room temperature before starting
operation (1 to 6h).

Make sure that the appliance connector and the plug of the supply cord are
accessible, so you can separate the instrument from the mains.

Make sure that the ventilation slots on the bottom and the rear are not obstructed
(see chapter 6.2). Make sure that there is no object underneath the Thermal Cycler
that may block the ventilation slots at the bottom (e.g. a piece of paper etc.)

There must be sufficient distance between the ventilation slots on the rear side of the
Thermal Cycler and a wall or another instrument (min 10 cm).
Ensure that both the rear and bottom ventilation slits of the
rear and bottom of the instrument are unobstructed.
Insufficient ventilation can cause overheating of the
instrument.

Make sure that the main supply voltage is in accordance with the label above the
power connection (see chapter 5.4)

Connect the Biometra TRIO Thermal Cycler to a grounded socket.
Instruction Manual TAdvanced TRIO 07/2015
17
Prior to connecting the unit to the power source, ensure that the voltage
selector at the back side of the instrument is set to the required voltage.
Danger of electric shock! Unplug the power cable before you open the
Biometra TRIO Thermal Cycler!

The display contrast can be adjusted to local light conditions (see chapter 12.1.5)
5.4 Operation Voltage
Important: Prior to connecting the Biometra TRIO Thermal Cycler to the mains, make sure
that the setting of the Voltage selector is in accordance with your mains Voltage.
The Biometra TRIO Thermal Cycler can operate at 100, 115 or 230 Volt. The operation
Voltage is shown on the Voltage selector which is located at the instrument bottom.
To change operation voltage, switch off the instrument and disconnect the mains plug.
Use a coin or another round shape item to turn the adjustment slot of the Voltage selector to
the new Voltage.
Instrument bottom
Instrument backside
Voltage
indicator
Mains plug
230
11
5
Fuses
110
Fuses
Adjustment
slot
18
1
Power switch
2
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6
Operating elements
6.1 The Biometra TRIO front view
Figure 2 Biometra TRIO front view
6.2 The Biometra TRIO rear view
At the Biometra TRIO backside the Ethernet port, mains plug and mains switch are located.
Figure 3 Biometra TRIO rear view
Instruction Manual TAdvanced TRIO 07/2015
19
6.3 High Performance Smart Lid (HPSL)
To achieve optimum pressure on the tubes the Biometra TRIO Thermal Cycler is equipped
with a height adjustable heated lid.
Close the lid:
After the samples have been placed in the block close the lid. Turn the wheel clockwise until
you hear a clicking noise. In this mode the pressure will not increase further, even when you
keep on turning the wheel.
Open the lid:
First: Release pressure by turning the wheel counter clockwise. As soon as there is no more
resistance the pressure has been released.
Then: Open the lid with by pushing the front button.
Important: The lid should not be opened under pressure because this leads to damage of
the locking mechanism.
6.4 Loading samples
The Biometra TRIO Thermal Cycler is an open system and compatible with strips, tubes or
plates. Regardless if plates are skirted, semi-skirted or non-skirted, if tubes have flat or
domed caps or if the plastic ware has 0.1 ml or 0.2 ml format, the Biometra TRIO accepts
almost all formats. By the high performance smart lid (HPSL) technology with clutch
mechanism the heated lid applies heat and force to the reaction vessels to prevent
condensation of the reaction mix and reliable contact pressure between plastic ware and
sample block.
After a run, the heated inner lid can remain hot. Use caution when opening and
closing the lid.
1. To open a lid, press on the marker indicated in Figure 1.
Figure 4 Biometra TRIO lid open button
2. Release the pressure on the button when the lid becomes unlocked
3. The lid opens automatically and stays open without assistance
4. Load the samples
Note: The heated lid force has been optimized for a fully loaded block. If only very few tubes
are loaded to the block place a dummy tubes each in the four corner positions to avoid
damage of tubes by excessive pressure.
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5. To close the lid push it down until it becomes arrested by the locking mechanism.
6.5 The Biometra TRIO Software
The Biometra TRIO features a 7‘‘ color touch screen and a completely new software as user
interface.
6.5.1
Keyboard
For some applications names, addresses or passwords have to be defined. For these
purposes the Biometra TRIO software offers a keyboard to enter characters, digits and
special characters.
Figure 5 Biometra TRIO keyboard
The keyboard offers the following special keys:
Space
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Tab
Shift
Delete single character
Delete all characters
Back
Forward
Note: For some functions the number of maximum characters is limited. The user names and
passwords can have maximal 13 characters, user initials maximal 3 characters and program
names maximal 13 characters.
6.5.2
Keypad
In some screens of the Biometra TRIO software digits have to be entered. For this purpose
the software features a virtual keypad integrated in some screens:
Figure 6 Biometra TRIO keypad
The keyboard offers to enter digits, dot and minus and infinite. Additionally the following
special keys are available:
Delete single character
Delete all characters
Tab
Infinite
6.5.3
22
Cursor keys
Instruction Manual TAdvanced TRIO 07/2015
The Biometra TRIO software uses lists, tables or graphical representations. Whenever the
number of entries becomes higher than the maximum number of steps or entries that can be
displayed, the software will show cursor keys for navigation.
6.5.4
Scroll up
Scroll Left
Scroll down
Scroll Right
Confirm or Cancel
For many screens user entries have to be confirmed to avoid unintended operating errors.
Press Confirm to accept settings/processes or press Cancel to refuse settings/processes.
Confirm
Cancel
6.5.5
List of used commands and icons
The Biometra TRIO thermal cycler software uses the following icons:
Command
Icon
Home
Back
Next
Select sample block 1
Select sample block 2
Select sample block 3
Right
Left
Up
Down
Confirm
Cancel
Log in
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User Management
User Management
ON/OFF
New User
Delete User
Edit User
Protocol Wizard
New Polymerase
Edit Polymerase
Delete Polymerase
Annealing temperature
calculator
Edit primer
concentration
Edit primer
Calculate primer
Save annealing
temperature Ta
Protocol Wizard Info
Incubation
Start Incubation
Programs
New Program
Open Template
Copy Program/Copy all
Programs
Delete Program
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Edit Program
Start Program
Choose block start
program
Stop Program
Choose block stop
program
Done
Save Program
Pause Program
Continue Program
Skip Step
Graphical view
Spreadsheet view
Temperature
Optimisation Step view
Edit Step
Insert Step
Delete Step
Temperature Increment
Time Increment
Ramp Rate
Temperature
Optimisation Step (TOS)
Preheating OFF
Preheating ON
Tools
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Settings
Date and Time
Automatic user logout
Beeper
Network
Network Settings
Network User
Display brightness
Display darker
Display brighter
Touch screen calibration
Factory settings
Documentation
Run-Logfile
Overview Run-Logfiles
Save Run-Logfile to
USB
Save all Run-Logfiles to
USB
View Run-Logfile
View Program
Selftest Logfile
Error Logfile
Extended Self-Test
Start Extended Self-Test
Stop Extended Self-Test
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Backup
Save Backup File
Load Backup File
Delete all Backup Files
Delete Backup File
Cycler Info
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7
Operating
7.1 Power On Self-Test
After the instrument is switched on the Biometra TRIO Thermal Cycler passes an initial selftest (power on self-test). While the instrument passes the test the start screen is shown
Figure 7 Biometra TRIO start screen
For the power on self-test a log file is created and stored in the Thermal Cycler memory (see
chapter 12.3.2).
7.2 Log in Screen
After the instrument has passed the power on self-test the log in screen is displayed. The log
in screen offers the following functions:
Command
Log in
Block
EN/DE
28
Function
Starts the log in dialog.
Shows the current block status. If a block is
Free a touch on the button leads to a list of
previously used programs for quick start for
the selected sample block (not user specific
before log in).
Changes the language setting for the log in
screen.
Chapter
See chapter 7.3
See chapter 7.5 and chapter
7.6
For user-specific language
settings see chapter 12.2.2
Instruction Manual TAdvanced TRIO 07/2015
Figure 8 Biometra TRIO log in screen
7.3 Log in
After starting the Biometra TRIO Thermal Cycler the log in screen is displayed (see chapter
7.1). To log in as existing user press
Login
In the next screen press the button to selected a user.
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Figure 9 Biometra TRIO select user screen
Note: The button position can change. The button for the latest logged in user is always
shown at the top left position of the screen. All other buttons become arranged from top left
to bottom right by the date and time of the latest user log in. If more than 6 user accounts are
available you might need to use the cursor keys Left or Right to turn the page (see chapter
6.5.3).
Touch the input box “Password” and enter the password using the Biometra TRIO keyboard
(see chapter 6.5.1). The default password for the administrator Admin is “Admin”. Please
note that the password query is case sensitive.
Figure 10 Biometra TRIO enter password screen
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Note: If a user directory is not password protected the software skips the enter password
screen (see Figure 10) and jumps directly into the home screen.
7.4 Home Screen
After user log in the home screen is shown by the Biometra TRIO software. The home
screen offers the following functions:
Figure 11 Biometra TRIO home screen
Command
Programs
Protocol
Wizard
Block
Incubate
Tools
Log out
Function
Viewing, running and editing programs
Automatic creation of PCR programs
Chapter
See chapter 8
See chapter 11
Shows the current block status. If a block is
Free a touch on the button leads to a userspecific list of the previously used programs
for quick start for the selected sample block
For running a sample block at a constant
temperature
Settings, documentation, extended self-test,
cycler info and diagnosis mode
User log out
See chapter 7.5 and chapter
7.6
See chapter 7.7
See chapter 11
7.5 Block status
In the log in screen (see chapter 7.2) and the home screen (see chapter 7.4) a special button
for the current sample block status is shown. The sample block status can be Free, Run or
Pause and is indicated by the color of the button and the textbox in the middle of the button
②. In the line above the textbox the current sample block temperature is shown ①. If the
temperature is higher than 70°C the characters are shown in red and the warning Hot plus
the corresponding warning symbol are displayed. In the line below the textbox the remaining
run time is shown ③. The block number is shown above the button.
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Figure 12 Biometra TRIO block status/quick start button
The following table summarizes the different button colors and sample block status. When
the button is pressed, it depends on the sample block status which function is activated by
the Biometra TRIO software. If the status is Run or Pause, a touch of the button will lead to
the program overview (see chapter 0). If the status is Free, the program quick start function
is activated (see chapter 7.6).
Button
Block Status
Color
Function
Free
Green
Quick start function
Run
Yellow
Program view
Blue
Program view
Pause
7.6 Quick start function
Note: Programs can only be started by users with the right Start/stop programs activated
(see chapter 12.2.2).
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The Biometra TRIO software offers a quick start function for previously used programs. To
use the quick start function, press the block status button in the log in screen (see chapter
7.2) or the home screen (see chapter 7.4).
Note: The quick start function can only be activated if the current block status is Free (see
chapter 7.5). Is the status is Run or Pause a touch of the button will lead to the program
overview screen instead.
If the block status button is used in the log in screen, before a user is logged in, the software
will show a list of latest used programs for quick start for the selected sample block. This list
is not user-specific. If the block button is used in the home screen, after user log in, the
software will show a user specific list of the latest used programs for quick start for the
selected sample block.
Log in screen
Home screen
Latest program list
User specific latest program list
The latest used programs are summarized in a table.
Figure 13 Biometra TRIO quick start screen
To switch between the sample blocks press
Sample block 1
Sample block 2
Sample block 3
Select and touch a program in the table for quick start. Press
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Start on block x
to start the selected program.
To start a program on multiple sample blocks press
Choose block start program
and by activating the checkboxes select the sample blocks to start.
Figure 14 Biometra TRIO multiblock selection query
If necessary the program can be edited before or a new program can be created using a
template (see chapter 8.1).
Note For programs including a TOS step (see chapter 8.6.5) all blocks are started
simultaneously and therefore the block selection option is not offered.
Note: Programs offered for quick start can only be edited or created by users with the right
“Write/delete own programs” activated (see chapter 12.2.1).
Press the corresponding button
Edit Program
New from template
to activate the programming mode or to select a program from the list of templates.
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7.7 Incubation Mode
Note: Incubations can only be started or stopped by users with the right Start/stop
programs activated (see chapter 12.2.2).
The incubation mode allows to incubate samples at a constant temperature for a defined
time. Press
Incubate
and in the next screen set the block temperature, hold time, heated lid on or off, heated lid
temperature and set preheating mode on or off using the virtual keypad and the
corresponding buttons (see Figure 15).
Note: To set the time to endless use button
.
To select a sample block press
Sample block 1
Sample block 2
Sample block 3
To start the incubation press
Start incubation
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Figure 15 Biometra TRIO incubation mode screen
During incubation the following commands can be used:
Stop Incubation
Choose block*
Pause Incubation
Continue Incubation
Skip Step
* the button “choose block stop program” for multiblock selection is only available if more than one sample block
is running.
Note: To stop the incubation, use the corresponding command. Do not switch off the
instrument while the incubation is running. The instrument will react like after a power failure
and will restart the incubation step when it is switched on next time.
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8
Creating, Editing and Saving programs
8.1 Programming modes
The Biometra TRIO software offers spreadsheet programming or graphical programming.
Use the buttons
Graphical view
Spreadsheet view
to toggle between the two modes (see Figure 16). The following screenshots show the
spreadsheet and graphical programming mode.
Spreadsheet programming
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Graphical programming
Figure 16 Biometra TRIO spreadsheet and graphical programming screen
Both programming modes show the same information but in different ways. In the
spreadsheet mode all parameters are summarized in a table. In the graphical mode for some
parameters symbols are displayed (see table below). Toggle to the spreadsheet mode to
view the settings for these parameters.
Parameter
Program name
Heated lid temperature [°C]
Preheat lid mode
Step number
Temperature [°C]
Holding time [hh:mm:ss]
Goto
Cycles
Temperature increment [T (°C)]
Time increment [t (s)]
Ramping rateR (°C/s)]
TOS step
Spreadsheet
mode
①
②
③
④
⑤
⑥
⑦, ⑫
⑧, ⑬
⑨
⑩
⑪
⑭
Graphical
mode
①
②
③
④
⑤
⑥
⑦
⑧
⑨
⑩
⑪
⑭
Chapter
See chapter 8.3
See chapter 8.4
See chapter 8.5
See chapter 8.6
See chapter 8.6
See chapter 8.6
See chapter 8.6.1
See chapter 8.6.1
See chapter 8.6.2
See chapter 8.6.3
See chapter 8.6.4
See chapter 8.6.5
If a program has more than 6 steps, use the cursor keys to navigate in the spreadsheet or
the graphical display (see chapter 6.5.3)
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8.2 Creating a new program/Use program template
Note: New programs can only be created by users with the right “Write/delete own
programs” activated (see chapter 12.2.1). Programs of other users can only be modified by
users with the right “Write/delete all programs” activated
1. To create a new program or to open a program template, first log in as existing user
(see chapter 7.1).
2. After log in press button
Programs
in the home screen (see chapter 7.3). The program overview screen opens:
Figure 17 Biometra TRIO program overview screen
3. In the program overview screen press button
New from template
4. The program template screen opens (see Figure 19). Search for a program template
using the cursor keys
Scroll up
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Scroll down
and touch the button to select it.
Note: The Biometra TRIO offers several program templates for different purposes. You may
use a template and modify the program as desired or you can select “Blank”. “Blank” is
program with no pre-defined steps (see Figure 18).
Spreadsheet programming
Graphical programming
Figure 18 Biometra TRIO program template “Blank”.
Select “Blank" whenever a new program should be created and select another template from
the list whenever an existing program should be edited.
The program structure can be controlled in the program preview. If a template is selected the
program steps and some additional information are displayed in the program preview table.
By using the program preview function the template can be checked before it is opened.
5. Press
Open template
to open a program template.
Figure 19 Biometra TRIO program template screen
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8.3 Set program name
Each template has a specific name which is superimposed in the programming screen. To
enter a new name touch the input box Name (see Figure 16) and enter a new name using
the Biometra TRIO virtual keyboard (see chapter 6.5.1).
Note: If a character is pressed, the pre-displayed name will be deleted and a new program
name can be set. If the program name should be modified please first press another button
like delete or space.
8.4 Set heated lid temperature
To set the heated lid temperature, touch the input field Lid (see Figure 16) and in the next
screen (see Figure 20) enter a value between 30°C and 110°C using the Biometra TRIO
virtual keypad.
Note: The default setting for the heated lid temperature is 99°C. 99°C is sufficient for typical
PCR programs.
To switch the heated lid on or off use the corresponding buttons.
Figure 20 Biometra TRIO lid temperature setting screen
8.5 Set preheating mode
The heated lid can be set to preheating ON or OFF using the corresponding buttons (see
Figure 16):
Preheating OFF
Preheating ON
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If the preheating mode is activated the heated lid is heated up to the target temperature while
the sample block is held constant at 25°C. After the heated lid reaches the target
temperature a 40 seconds equilibration phase follows. Then the sample block starts to heat
up from 25°C to the first programmed temperature.
Note: For most applications it is recommendable to set the preheating function to “ON”.
8.6 Edit parameter or step
The software offers two different options to edit program steps or single parameters:
1. In the programming spreadsheet or the graphical view single parameters can be
activated and values entered.
2. To edit all parameters in a program step the software offers the edit step screen (see
Figure 21). Furthermore in this screen it can be switched between steps and
parameters entered for multiple steps (Multi Step Programming).
Activate a parameter in the tabular or graphical view (see chapter 8.1) and enter the desired
values or select a program step and press
Edit Step
The following parameters can be edited:
Parameter
Description and ranges
Chapter
Temperature in °C for a step. The target temperature
Temperature can be set between 3.0°C and 99.0°C in tenths of a
See chapter 8.6
degree.
Hold time for a step in hh:mm:ss. Between 0 and 8
See chapter 8.6
hours, 0 and 59 minutes and 0 to 59 seconds can be
set.
Hold time
To program a pause and set the holding time
indefinitely, enter ∞ in one of the input boxes for
hh:mm:ss.
Goto
Defines the step number the program jumps back to.
See chapter 8.6.1
Defines the number of repeats. The maximum
See chapter 8.6.1
Cycles
number of repeats is 999.
Temperature increment to increase or decrease the
See chapter 8.6.2
set temperature with each cycle by the defined value.
The increment can be set to ± 20°C.
Time increment (e.g. for long range PCR). Increases
See chapter 8.6.3
the set time with each cycle by the defined
increment. The increment can be set from 0s to
240s.
Average ramp rate between steps. The average
See chapter 8.6.4
ramp rate can be set from 0.1°C/s to max in tenths of
a degree (The maximum ramp rate is sample block
specific). The ramp rate specifies the speed at which
the selected step is reached with.
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Temperature Optimisation Step (TOS). For
optimisation of new PCR protocols very often
gradient enabled Thermocyclers are used. The
Biometra TRIO offers no gradient function but by the
temperature optimisation step (TOS) function
pograms making use of the three blocks and
delivering different annealing temperatures at the
defined step can be created.
See chapter 8.6.5
Figure 21 Biometra TRIO edit step screen
Note: To program a step at least the temperature and the hold time must be defined.
To set the temperature touch the corresponding input box and enter a value between 3.0°C
and 99.0°C. Use the Tab button or touch the corresponding input box to activate the next
input box and enter the time in h:mm:ss.
The Biometra TRIO software offers to program several steps subsequently. Use the buttons
Previous step
Next step
to navigate between the steps. The currently activated step is highlighted in the program
overview table (see Figure 21).
Note: By navigating between steps it is not necessary to confirm the settings for each single
step and to leave the edit step screen but you can fill in the parameters for several steps and
confirm the settings after all steps are programmed.
8.6.1
Set cycles
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A typical PCR program consists of repeated steps for denaturation, annealing and extension.
For the repetition of steps the Biometra TRIO software offers to program loops. For a cycle
two parameters have to be set:
1. The step number to go back to (Goto)
2. The number of repeats (Cycles)
To program a loop select the last step of the cycle and enter the step number the program
shall go back to in the input box “Goto” (see Figure 21). To define the number of repeats,
enter a number in the input box “Cycles” (see Figure 21).
For example to program the cycles for the following protocol select step 4, enter 2 in the
input box “Goto” and enter 35 in the input box “Cycles”.
Step No.
1
2
3
4
5
6
Temp.
94 °C
94 °C
55 °C
72 °C
72 °C
16 °C
Time Cycles
05:00
01:00
01:00
35x
01:00
05:00
Pause
In this example the instrument first executes step 1 to step 4, then repeats step 2 to step 4
for 34 times, performs the final extension step at 72°C and holds the temperature indefinitely
at 16°C.
Note: The Biometra TRIO allows to set maximal two interlaced loops. It is possible to
program a loop within a loop but it is not allowed to set loop in loop in a loop.
8.6.2
Set temperature increment
For some applications, like for example touch down PCR, temperature increments are used.
The temperature is increased or decreased with each cycle by the set value. To program a
temperature increment press
Temperature Increment (T)
(see Figure 21) and fill in a value between +20°C and -20°C in the corresponding input box.
Note: Temperature increments only become active in repeated steps (cycles). If a
temperature increment has been programmed, the small symbol
will be shown at the
corresponding step in the graphical programming mode.
8.6.3
Set time increment
For some applications, like for example long range PCR, time increments are used. The time
is increased with each cycle by the set value. To program a time increment press
Time Increment (T)
(see Figure 21) and fill in a value between 0s and 240s in the corresponding input box.
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Note: Time increments only become active in repeated steps (cycles). If a time increment
has been programmed, the small symbol
will be shown at the corresponding step in the
graphical programming mode.
8.6.4
Adjust ramp rate
The average ramp rate can be adjusted for each step. The ramp rate adjustment can be
useful if PCR programs are transferred from slower Thermal Cyclers the Biometra TRIO or
for special PCR applications like for example telomerase PCR. The average ramp rate
specifies the speed at which the selected step is reached with. To adjust the ramp rate press
Ramp Rate (R)
(see Figure 21) and fill in a value between 0.1°C/s and max in tenths of a degree.
Note: The maximum average rate depends on the sample block installed in the Biometra
TRIO Thermal Cycler. If the ramp rate has been adjusted, the small symbol
will be shown
at the corresponding step in the graphical programming mode.
8.6.5
Set Temperature Optimisation Step (TOS)
For optimisation of new PCR protocols very often gradient enabled Thermocyclers are used.
The Biometra TRIO offers no gradient function but by the temperature optimisation step
(TOS) function pograms making use of the three blocks and delivering different annealing
temperatures at the defined step can be created.
Sample replicates are distributed across the sample blocks and are tested for the
temperature giving the optimal result. To program a Temperature Optimisation Step press
Temperature Optimisation Step (TOS)
(see Figure 21), enter the desired annealing temperature (Annealing Temp.) and define a
temperature increment ( Increment) between the sample blocks. The effective temperatures
for all sample blocks are displayed (see Figure 22).
Note: The annealing temperature is set for sample block 2 and the increment defines the
temperatures for sample block 1 and 3. When setting a positive increment the temperature in
sample block 1 is the lowest and the temperature in sample block 3 is the highest. Use the
minus button on the keypad to enter a negative increment. When a negative value is used as
increment the temperature in sample block 1 is the lowest and the temperature in sample
block 3 is the highest.
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Figure 22 Biometra TRIO Temperature Optimisation Step screen
8.7 Insert step
To insert a new step, select a program step in the Biometra TRIO programming screen (see
Figure 16) and press
Insert Step
By using the button Insert Step the Edit Step function (see chapter 8.6) is activated. Set the
parameters for the new step.
Note: The new step is inserted at the selected program step. If for example step 3 is
selected, the new step will be inserted at step 3. The existing step 3 is not overwritten, but
instead step 3 and all following steps will be moved one position higher. This means, the
existing step 3 will become step 4, step 4 will become step 5 and so on.
Note: If the new step is inserted to an existing loop, the number of steps within the loop
increases, whereas the number of cycles remains the same.
8.8 Delete step
To delete a step, select a program step in the Biometra TRIO programming screen (see
Figure 16) and press
Delete Step
Note: If a step is deleted, all following steps will be moved one position lower. If for example
step 3 is deleted, step 4 will become step 3, step 5 will become step 4 and so on.
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Note: If the step is deleted in an existing loop, the number of steps within the loop is
reduced, whereas the number of cycles remains the same.
8.9 Save Program
To save a program press
Done
in the Biometra TRIO programming screen (see Figure 16). In the next screen (see Figure
23) touch the corresponding buttons to select a user directory and a storage place for the
program. If the number of user directories or programs is higher than can be maximal
displayed in the list, the buttons above and below the user and program list can be used to
scroll up and down.
Note: The user directory of the logged in user is automatically pre-selected. Users with the
right “Write/delete all programs” (see chapter 12.2.1) can also select directories of others to
save programs.
Note: If a new program is to be saved, the first free storage place in the user directory of the
logged in user is pre-selected. However, the storage place can be modified by using the
cursor key function above and below the list. If the selected storage place is already in use,
the stored program will not be overwritten, but instead the program stored at the selected
storage place and all other following programs will be moved to a higher storage place. The
storage place number for these programs will increase by +1 and the new program is saved
at the selected storage place.
After a user directory and storage place are selected, press
Save
in the Biometra TRIO program overview screen (see Figure 23) to save the program.
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Figure 23 Biometra TRIO program overview screen
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9
Starting, Copying and Deleting programs
9.1 Start program
Note: Programs can only be started by users with the right “Start/stop programs” activated
(see chapter 12.2.2). Additionally the right “Read all programs” defines, if a user is allowed
to read all programs or if the access is limited to own programs. A user who is not allowed to
read all programs cannot start programs of others even if the right “Start/stop programs” is
activated.
The Biometra TRIO software offers a quick start function for previously used programs. The
quick start function is described in chapter 7.6. This chapter deals with the start of stored
programs by selecting a program from a user account. To select a program for start, press
Programs
in the Biometra TRIO home screen (see chapter 7.4). The program overview screen opens
with the user directory of the logged in user pre-selected (see Figure 24).
Figure 24 Biometra TRIO program overview screen
Touch the corresponding buttons to select a user directory and a stored program. If the
number of user directories or programs is higher than can be maximal displayed in the list,
the buttons above and below the user and program list can be used to scroll up and down.
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For the selected program a preview opens with general information on the program and a list
of the steps (see Figure 25). The program preview can be used to check the program prior to
start. To start the selected program press
Start Program
Figure 25 Biometra TRIO program overview screen with program preview
In the multiblock selection query select the sample block to start
Figure 26 Biometra TRIO multiblock selection query
and confirm your selection. The program will be started on the selected sample blocks.
Note For programs including a TOS step (see chapter 8.6.5) all blocks are started
simultaneously and therefore the block selection option is not offered.
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9.2 Copy program
Note: Own programs can only be copied and saved by users with the right “Write/delete
own programs” activated. Programs of others can be copied and saved with the right
“Write/delete all programs” activated (see chapter 12.2.2).
To copy a program, touch the corresponding buttons to select a user directory and a stored
program in the Biometra TRIO program overview screen (see Figure 24). If the number of
user directories or programs is higher than can be maximal displayed in the list, the buttons
above and below the user and program list can be used to scroll up and down. To copy the
selected program press
Copy
Touch the corresponding buttons to select a user directory and a storage place in the
Biometra TRIO program overview screen (see Figure 27). The program to be copied is
highlighted in green. If the number of user directories or programs is higher than can be
maximal displayed in the list, the buttons above and below the user and program list can be
used to scroll up and down. To save the program press
Save
Note: If the selected storage place is already in use, the stored program will not be
overwritten, but instead the program stored at the selected storage place and all other
following programs in the user directory will be moved to a higher storage place. The storage
place number for these programs will increase by +1 and the program is copied to the
selected storage place.
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Figure 27 Biometra TRIO program overview screen
9.3 Copy all programs
Note: Own programs can only be copied and saved by users with the right “Write/delete
own programs” activated. Programs of others can be copied and saved with the right
“Write/delete all programs” activated (see chapter 12.2.2).
To copy all programs of a user directory, touch the corresponding button to select a user
directory in the Biometra TRIO program overview screen (see Figure 24). If the number of
user directories is higher than can be maximal displayed in the list, the buttons above and
below the user list can be used to scroll up and down. To copy all programs press
Copy all
Touch the corresponding button to select a user directory in the Biometra TRIO program
overview screen (see Figure 28). If the number of user directories is higher than can be
maximal displayed in the list, the buttons above and below the user list can be used to scroll
up and down. To save the programs press
Save
Note: The programs will be copied into the selected user directory beginning at the first free
storage place.
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Figure 28 Biometra TRIO program overview screen
9.4 Delete program
Note: Own programs can only be deleted by users with the right “Write/delete own
programs” activated. Programs of others can be deleted with the right “Write/delete all
programs” activated (see chapter 12.2.2).
To delete a program, touch the corresponding buttons to select a user directory and a stored
program in the Biometra TRIO program overview screen (see Figure 24). If the number of
user directories or programs is higher than can be maximal displayed in the list, the buttons
above and below the user and program list can be used to scroll up and down. To delete the
selected program press
Delete
Confirm the security query to delete the program.
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Figure 29 Biometra TRIO security query
Note: If a program is deleted, all other following programs in the user directory will be moved
to a lower storage place. The storage place number for these programs will decrease by -1.
9.5 Delete all programs
Note: Own programs can only be deleted by users with the right “Write/delete own
programs” activated. Programs of others can be deleted with the right “Write/delete all
programs” activated (see chapter 12.2.2).
To delete all programs, touch the corresponding button to select a user directory in the
Biometra TRIO program overview screen (see Figure 24). If the number of user directories is
higher than can be maximal displayed in the list, the buttons above and below the user can
be used to scroll up and down. To delete all programs in the user directory press
Delete all
Confirm the security query to delete all programs.
Figure 30 Biometra TRIO security query
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10 Running, Pausing, Continuing and Stopping programs
To switch between the view for the sample blocks during run press
Sample block 1
Sample block 2
Sample block 3
in the corresponding screen.
10.1 Display during run
After a program is started (see chapter 9.1), the Biometra TRIO software can display the
running program in spreadsheet or graphical view. Use the buttons
Graphical view
Spreadsheet view
to toggle between both modes.
Graphical view
Spreadsheet view
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Figure 31 Biometra TRIO run screen in graphical view and spreadsheet view.
The following parameters are summarized in the headline table:
Program Name
Estimated End
Remaining Time
Sample Block Temperature
Lid Temperature
Step Number
If the heated lid is set to preheating ON (see chapter 8.5), during the preheating of the
heated lid the word “Preheating” will be displayed in the field Step in the headline table. After
the heated lid has reached the target temperature and the 40s equilibration phase has
passed, the first program step starts. During program run, the current step is highlighted in
yellow in Graphical view and Spreadsheet view. In graphical view, each step is divided in
heating phase and holding time and both parts are highlighted separately. Time and
temperature increments and ramping rate adjustments cannot be shown in Graphical view.
Instead the symbols T, t or ! are displayed (see chapter 8.1).
10.2 Pause Program
To pause a program press
Pause
in the Biometra TRIO run screen (see Figure 31). The word “Pause” is shown and in the field
remaining run time the pause time is counted.
Spreadsheet view
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Graphical view
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Figure 32 Biometra TRIO run screen during program pause
Note: To press the pause button requires manual interaction. The Biometra TRIO software
also allows to program a pause step (see chapter 8.6), then the pause step is automatically
started by the instrument.
Note For programs including a TOS step (see chapter 8.6.5) all blocks are paused
simultaneously.
Note: If the instrument is switched off during a pause step, this will be recognized as a power
failure (a referring error message is written to the Run-Logfile). For example, if the last step
in a program is a pause, the program has to be stopped by pressing the Stop button (see
chapter 10.5) before switching off the instrument.
10.3 Continue Program
To continue a paused program press
Continue
in the Biometra TRIO run screen during program pause (see Figure 32). The Biometra TRIO
Thermal Cycler will continue the program exactly from the point when the pause was
initiated.
Note For programs including a TOS step (see chapter 8.6.5) the program is continued on all
blocks simultaneously.
10.4 Skip Step
To skip a step program press
Skip
in the Biometra TRIO run screen (see Figure 31). The Biometra TRIO Thermal Cycler will
immediately continue with the next program step and skips the current step.
10.5 Stop Program
Note: Programs can only be stopped by users with the right “Start/stop programs” activated
(see chapter 12.2.2).
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To stop a program press
Stop program on block x
in the Biometra TRIO run screen (see Figure 31). If two or more sample blocks are running
the Biometra TRIO thermal cycler offers the option to stop multiple sample blocks
simultaneously. Press
Choose block stop program
in the Biometra TRIO run screen (see Figure 31) and activate the corresponding check
boxes to select the sample blocks to stop.
* the button “choose block stop program” for multiblock selection is only available if more than one sample block
is running.
Figure 33 Biometra TRIO query for multiblock selection
Confirm the block selection and the following security query to stop the running program(s).
Figure 34 Biometra TRIO security query
The Biometra TRIO Thermal Cycler will immediately stop the running sample block(s).
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11 Protocol Wizard
The protocol wizard is a helpful tool for automatic creation of PCR programs and primer
annealing temperature calculation. To start the protocol wizard press
Protocol Wizard
in the Biometra TRIO home screen (see chapter 7.4).
11.1 Select Polymerase
The protocol wizard uses polymerase-specific program templates to create PCR programs.
Program templates for up to 8 polymerases can be stored by the Biometra TRIO software.
Figure 35 Biometra TRIO polymerase selection screen
Pre-installed in the software is the program template for Analytik Jena Innupure
polymerases. To create a PCR program select a polymerase from the list and press
Next
In the next screen (see chapter 11.3) enter values for the requested parameters to continue
with the automatic program creation.
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11.2 Enter New Polymerase or Edit Polymerase
To enter program templates for new polymerases or to edit preset program templates for
polymerases press the corresponding button:
New Polymerase
Edit Polymerase
In the next screen (see figure Figure 36) touch the corresponding input boxes to enter a
name for the polymerase and set parameters for all requested program steps. With two
exceptions for most program steps the temperature and the holdtime must be defined:
1. The temperature for the annealing step depends on the calculated primer melting
temperature (Tm-value) and has to be set individually for each primer pair (see
chapter 11.4).
2. The holdtime for the elongation step becomes calculated by the expected product
length (see chapter 11.4).
The corresponding input boxes for the annealing temperature and elongation time are shown
in grey and cannot be activated.
Figure 36 Biometra TRIO screen for editing program templates for polymerases
After creating the program template for a new polymerase or editing the program template of
a polymerase press
Done
and in the next screen (see Figure 37) touch the corresponding button to select a storage
place for the program template (polymerase). If the number of polymerases is higher than
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can be maximal displayed in the list, the buttons above and below the polymerase list can be
used to scroll up and down.
Figure 37 Biometra TRIO for saving program templates for polymerases
Note: If a new or edited polymerase is to be saved, the first free storage place in the
polymerase list is pre-selected. However, the storage place can be modified by using the
cursor key function above and below the list.
To save the new or edited polymerase press
Save
Note: If the selected storage place is already in use, the stored polymerase will be
overwritten. Confirm the security query to overwrite the existing polymerase:
Figure 38 Biometra TRIO security query
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11.3 Delete Polymerase
To delete a polymerase press
Delete Polymerase
Note: The Template “InnuTaq” cannot be deleted.
11.4 Protocol Wizard parameter screen
For automatic calculation of PCR programs in the protocol wizard parameter screen (see
Figure 39) some parameters have to be set by the user:
1. Touch the corresponding checkbox to select between two step and three step
programs.
Note: The protocol wizard offers to create two step or three step PCR programs. A
three step program consists of separated steps for denaturation, annealing and
extension. In a two step program the annealing and extension step are combined to
one single step. In consequence for a three step protocol different temperatures for
the annealing and extension step are used and for a two step program the primer
annealing temperature Ta (see below) is used for the annealing and extension step.
2. Enter the PCR product length
Note: The product length is used by the software to calculate the elongation time.
The software uses the following equation:
Elongation time [s] = product length [bases]/1000 [bases]*60s
For very short products the minimum elongation time is set to 6s.
3. Enter the primer annealing temperature
Note: In general the annealing temperature (Ta) should be set approximately 5°C
below the calculated mean primer pair melting temperature (Tm). The theoretical Tavalue can also be calculated by the Biometra TRIO protocol wizard (see chapter
11.5).
4. Enter the number of cycles
To set the parameters touch the corresponding field in the protocol wizard parameter screen
and enter the desired values.
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Figure 39 Biometra TRIO protocol wizard parameter screen
After setting all program parameters press
Next
The next screen lists the all steps of the program that will be created by the software:
Figure 40 Biometra TRIO protocol wizard view parameter screen
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Press
Create program
to enter the Biometra TRIO programming screen (see chapter 8). In the programming screen
the program is displayed in graphical or spreadsheet mode and can be edited. To save the
program created by the protocol wizard follow the instructions in chapter 8.9.
Note: Programs have to be saved before they can be started. If the program has been
created but should not be saved confirm the following security query to discard the program:
Figure 41 Biometra TRIO security query
11.5 Primer Annealing Temperature Calculator
Based on the primer sequences the Biometra TRIO protocol wizard can calculate the
theoretical primer annealing temperature (Ta). Press button
Calc Ta
in the Biometra TRIO protocol wizard parameter screen (see Figure 39) to access the primer
annealing temperature calculator screen (see Figure 42). In the annealing temperature
calculator screen enter the sequences for the forward and reverse primer using the button
below the input fields. The sequence length is counted by the software and displayed in
brackets left to the input fields.
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Figure 42 Biometra TRIO primer annealing temperature calculator screen
To adjust the primer or salt concentration press
Edit concentration
In the next screen the primer concentration, salt concentration and magnesium concentration
can be adjusted. Touch the corresponding input field and enter values using the keypad. The
concentrations have the following default values and ranges:
Function
Primer concentration
Salt concentration
Magnesium concentration
Default value
200 nM
50 mM
0 mM
Instruction Manual TAdvanced TRIO 07/2015
Range
0-2.000 nM
0-200 mM
0-200 mM
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Figure 43 Biometra TRIO screen for primer concentration adjustment
Press
Edit primer
to toggle to the primer annealing temperature calculator screen (see Figure 42) or press
Calc. primer
to calculate the theoretical primer annealing temperature (Ta). The calculated values for the
forward primer melting temperature (Tm), reverse primer melting temperature (Tm), mean
primer melting temperature (Tm) and calculated annealing temperature (Ta) are displayed.
Press
Save temp.
to transfer the calculated primer annealing temperature to the protocol wizard (see Figure
39). The calculated Ta value will be displayed in the input box for the primer annealing
temperature.
Press
Info
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to get information on the algorithms used for annealing temperature calculations.
For short sequences of 14 nucleotides or less the software calculates the primer annealing
temperature (Ta) based on the primer Tm values as follows:
1. For each primer the melting temperature is calculated using the following equation
according to Wallace et al.1:
Tm = ((w*A + x*T)*2) + ((y*G + z*C)*4)
where w, x, y and z are the number of the bases A, T, G and C in the primer
sequence, respectively.
2. The average Tm value is calculated from the Tm values for both primer sequences
3. The annealing temperature Ta is calculated based on the average Tm value by
applying the following rules:
- If the difference between the primer Tm values is 4°C or less, the Ta is calculated
as the average Tm value minus 5°C
- If the difference between the primer Tm values is higher than 4°C, the Ta is
calculated as the lower Tm value plus 2°C.
For long primer sequences of 15 nucleotides or more the software calculates the primer
annealing temperature (Ta) based on the nearest neighbor method according to SantaLucia2
and von Ahsen et al.3.
1
Wallace et al., Nucleic Acids Res. 6, 3543, 1979
2
SantaLucia, Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1460-5
von Ahsen et al., Clin Chem. 1999 Dec;45(12):2094-101
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12 Tools
The Biometra TRIO software provides the following tools:
Settings
Define basic instrument settings
See chapter 12.1
User Management
Tool for the user management
See chapter 12.2
Documentation
Access to stored log files
See chapter 12.3
Extended Self-Test
Execute extended self-test
See chapter 12.4
Service Info File (SINF) Create Service Info File
See chapter 12.5
Backup
Saves all folders, programs, users and See chapter 12.6
user settings to USB
Cycler Info
Provides general information on the See chapter 12.7
instrument
Contact
Biometra contact data
See chapter 12.8
Figure 44 Biometra TRIO tools screen
Press the corresponding button to access the desired function.
12.1 Settings
Note: System settings can only be modified by users with the right “System configuration”
activated (see chapter 12.2.2). The system configuration affects all functions listed below
expect of the user management.
The Biometra TRIO software offers the following settings tools:
Date and Time
Set date and time
See chapter 12.1.1
Automatic user
Switch automatic user logout
See chapter 12.1.2
logout
on or off and time settings
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Beeper
Network
Display Brightness
Touch Screen
Calibration
Factory Settings
Switch beeper on or off
Define network settings or list
network users
Adjust display contrast
Calibrate touch screen
Reset the settings
See chapter 12.1.3
See chapter 12.1.4
See chapter 12.1.5
See chapter 12.1.6
See chapter 12.1.7
Figure 45 Biometra TRIO settings screen
Press the corresponding button to access the desired function.
12.1.1 Set Date and Time
Note: Date and time can only be edited by users with the right “System configuration”
activated (see chapter 12.2.2).
The Biometra TRIO software allows the user to set time and date. The date is defined in
day:month:year and the time in hours:minutes:seconds.
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Figure 46 Biometra TRIO date and time screen
Activate the corresponding input box and use the keypad on the right side of the date and
time screen to enter digits. Additionally the following keys might be helpful to fill out the input
boxes:
Delete single character
Delete all characters
Jump to the next input box
Note: The following keys are inactive in the date and time screen and cannot be used:
12.1.2 Automatic user logout
Note: The automatic user logout can only be configured by users with the right “System
configuration” activated (see chapter 12.2.2).
The Biometra TRIO Thermal Cycler features an automatic user logout. The automatic user
logout can be switched ON or OFF by using the corresponding buttons:
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Automatic user logout ON
Automatic user logout OFF
If the automatic user logout is switched ON the time after that the user is automatically
logged out can be set. You can enter values between 30 minutes and 60 minutes.
Figure 47 Biometra TRIO automatic user logout screen
Note: The standard setting is automatic user logout OFF.
Note: The following keys are inactive in the automatic user logout screen and cannot be
used:
12.1.3 Configure Beeper
Note: The beeper can only be configured by users with the right “System configuration”
activated (see chapter 12.2.2).
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The Biometra TRIO Thermal Cycler has a beeper and gives an acoustic signal if a PCR
program is finished and the beeper is turned ON. The beeper can be switched ON or OFF by
activating the corresponding check box.
Figure 48 Biometra TRIO beeper configuration screen
12.1.4 Network
The menu item Network allows to configure the network settings and to show a list of
network users. Press the corresponding button
Network Settings
Network User
to access a function.
12.1.4.1 Network settings
Note: The network settings can only be edited by users with the right “System
configuration” activated (see chapter 12.2.2).
The network settings allow to set a name for the thermal cycler used in the network. The
default setting is the instruments serial number. Touch the input box and use the Biometra
TRIO keyboard (see chapter 6.5.1) to enter a new name.
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Note: The name can have a length up to 16 characters.
The Biometra TRIO Thermal Cycler can handle static and dynamic IP addresses. Activate
the corresponding check box to choose between dynamic (DHCP) and static IP address. If
DHCP is activated the instrument automatically receives the network configuration settings. If
the check box static is activated you can enter the IP address, the subnet mask and the port
by using the keypad on the right side of the screen.
The following keys might be helpful to fill out the input boxes:
Delete single character
Delete all characters
Jump to the next input box
Figure 49 Biometra TRIO network settings screen
Note: The network cable must be at least performance class Cat 5e and the cable
configuration has to be STP.
12.1.4.2 Network user
The software stores the network users and lists them in chronological order in a table. By the
function it is possible to check if only authorized persons have network access to the
instrument.
12.1.5 Display brightness
Note: The display brightness can only be edited by users with the right “System
configuration” activated (see chapter 12.2.2).
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The display brightness can be adjusted to the local light conditions. To adjust the brightness
use the corresponding buttons:
Darker
Brighter
or use the horizontal slider.
Figure 50 Biometra TRIO display brightness settings screen
12.1.6 Touch screen calibration
Note: The touch screen can only be calibrated by users with the right “System
configuration” activated (see chapter 12.2.2).
To calibrate the touch screen follow the instructions and touch the screen at the center of the
displayed circle. The procedure is repeated three times at different positions of the display. If
at the end of the procedure a small cross is shown within the circle the calibration is
successful.
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Figure 51 Biometra TRIO touch screen calibration screen
12.1.7 Factory settings
Note: The factory settings tool can only be executed by users with the right “System
configuration” activated (see chapter 12.2.2).
Press the corresponding button and confirm the following security query:
Figure 52 Biometra TRIO security query
By using the factory settings tool all user accounts and programs will be deleted!
Note: Use a backup file (see chapter 12.6) to restore the system.
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12.2 User Management
The Biometra TRIO thermal cycler manages up to 30 individual user accounts. The user
management allows to create new users and to set rights for users. The user management is
a complex tool. Please follow the general guidelines:
1. The factory setting for the administrator password is “Admin”. Please change the
password after switching on user management to protect the system from unwanted
modifications.
2. Use the backup tool to create backup files. Backup files can be used to restore a
system or to synchronize the memory contents of Biometra TRIO thermal cyclers.
3. Do not switch off the user management if you are not absolutely sure. All rights
settings will get lost and can only be restored if a backup file is available.
The user management can be switched on or off. By default the user management is
switched off after delivery.
To set the user management on or off press
User Management
in the Biometra TRIO user management screen (see Figure 53).
Note: The button User Management is only available for the user group “Administrator”.
Only an administrator can switch on or off the Biometra TRIO user management. Also with
the user management switched off the administrator “Admin” has to log in.
Figure 53 Biometra TRIO user management screen
In the next screen activate the corresponding checkbox to configure the Biometra TRIO user
management:
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Figure 54 Biometra TRIO configure user management screen
If the user management is switched off, all new users have all rights and can access all
software functions. With the user management switched on, the Biometra TRIO Thermal
Cycler supports 3 different user groups with pre-defined rights:
Administrator
User with generic
rights
User with limited
rights
System configuration
x
Edit user
x
Create user
x#
Delete user
x
Write/delete all
programs
x
Write/delete own
programs
x
x
Read all programs
x
x
x
Start/stop programs
x
x
x
x#
#
an administrator can create users of all groups and modify their rights settings. A user with
generic rights is only allowed to create users with generic or limited rights but no
administrator. In addition, the user with generic rights cannot modify the rights settings of
other users.
The settings for each user can be modified (see chapter 12.2.2) by the administrator and will
be stored by the Biometra TRIO software.
Note: If the user management is switched off, user accounts and programs are not protected
and can be modified or deleted by others.
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Available user accounts are listed in the Biometra TRIO user management screen (see
Figure 53) sorted by the date and time of the lasted log in and the user name, initials and
group are shown. Depending on the settings a user can be grouped as
1.
2.
3.
4.
Administrator
User with generic rights
User with limited rights
User defined
If more than 8 user accounts are available you might need to scroll the table up or down to
get see your desired user account (see Figure 53).
Scroll up
Scroll down
To select a user account press on the corresponding line in the table.
12.2.1 Create user
Note: New users can only be created by users with the right Create User activated (see
chapter 12.2.2).
An administrator can create users of all groups and modify their rights settings. A user with
generic rights is only allowed to create users with generic or limited rights but no
administrator. In addition, the user with generic rights cannot modify the rights settings of
other users.
To create a new user press
New User
In the next screen enter
- The user name (up to 13 characters or numbers)
- Initials (2 to 3 characters)
- Password (up to 13 characters, numbers or special characters)
- Repeat Password
using the Biometra TRIO keyboard (see chapter 6.5.1) and set the language by activating the
corresponding check box.
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Figure 55 Biometra TRIO create user screen
Note: The password protection is optional. However, if no password is used, your account
containing all of your programs might be modified or deleted by unauthorized persons.
Note: The language settings are user specific. For each user the language setting becomes
saved by the instrument. The language setting can be changed during operation and the
instrument does not be switched off and rebooted.
12.2.2 Edit user
Note: User rights can only be edited by users with the right “Edit User” activated (see
chapter 12.2.2).
To edit the user properties select a user account in the user management screen (see
chapter 12.2) and press the button
Edit user account
In the next screen the user data and the language settings can be changed.
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Figure 56 Biometra TRIO user data screen
Note: For the administrator “Admin” the user name and initials “ADM” are fixed and cannot
be modified. It is highly recommendable to change the password for the administrator
„Admin“ after the first system start und to create a backup file. Directories, programs users
and right settings can be restored from a backup file.
To modify the user rights press
User properties
In the next screen the user can be defined as
1. Administrator
2. User with generic rights
3. User with limited rights
For each user group templates defining the user rights are available. However, the predefined settings can be modified. You can activate or deactivate single rights. Activated
rights are highlighted blue, deactivated rights are shown in grey. The settings for each user
will be stored by the Biometra TRIO software and are shown in the Biometra TRIO user
management screen (see chapter 12.2).
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Figure 57 Biometra TRIO user properties screen
Right
Function
System configuration
Provides access to several system settings like date and time,
screen saver, beeper, network settings, display brightness, touch
screen calibration and factory settings
Edit user
Allows to modify the user name, user initials, password, language
setting and user rights
Create user
Allows to create new user accounts. A user with generic rights
can only create generic or limited users and is not allowed to
modify the rights settings. An administrator is allowed to create all
user types and to modify the rights settings for each user.
Delete user
Allows to delete existing user accounts
Write/delete all
programs
The user can edit/copy and delete all programs of all users
Write/delete own
programs
The user can edit/copy and delete own programs but not of other
users
Read all programs
If inactivated the access is limited to own programs. Programs of
others cannot be viewed (if a program cannot be viewed it also
cannot be edited or started by the user).
Start/stop programs
The user can start or stop programs
Note: The settings for the administrator “Admin” cannot be changed. The administrator
“Admin” always has all rights since at least one user needs to have all rights.
Press
User data
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to go back to the create user screen.
12.2.3 Delete user
Note: User accounts can only be deleted by users with the right “Delete User” activated (see
chapter 12.2.2).
To delete a user select the user account in the user management screen (see chapter 12.2)
and press the button
Delete user account
Note: The administrator “Admin” cannot be deleted since at least one user must be
administrator of the Biometra TRIO Thermal Cycler.
Confirm the security query to delete the selected user.
Figure 58 Biometra TRIO security query
The user and all of his programs will be deleted.
12.3 Documentation
The Biometra TRIO software offers the following documentation tools:
Run-Logfile
Power On Logfile
Self-Test Logfile
Error Logfile
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Documentation of PCR runs
Results of the latest initialisation test
Results of extended self-tests
Lists all errors detected by the instrument
See chapter 12.3.1
See chapter 12.3.2
See chapter 12.3.3
See chapter 12.3.4
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Figure 59 Biometra TRIO documentation screen
Press the corresponding button to access the desired function.
12.3.1 Run-Logfile
The Biometra TRIO Thermal Cycler lists up to 6 Run-Logfiles for the latest started programs.
Figure 60 Biometra TRIO Run-Logfile overview screen
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The Run-Logfiles are sorted by date and time. The latest started program is shown in
position 1 in the table. Additionally the user that started the program, the sample block
number used to run the program, the program name, directory and number are shown.
To view the collected data select a Run-Logfile from the list and press button:
View Run-Logfile
In the Run-Logfile screen the following information is listed:













Run-Logfile name
Log time and date
Start and end time of the program run
Date of the program run
Name of the vendor
Software version
User initials
Cycler type
Serial number Cycler
Block type
Block serial number
Block number
Messages
Figure 61 Biometra TRIO Run-Logfile screen
To view the corresponding program press button:
View Program
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The Biometra TRIO offers the option to show the program in graphical from or as
spreadsheet (see chapter 8.1). To toggle between graphical and spreadsheet view use
buttons:
Graphical view
Spreadsheet view
Figure 62 Biometra TRIO View program screen
Use the buttons
Overview Run-Logfiles
View Run-Logfile
View Program
to toggle between the overview screen, view Run-Logfile screen and view program screen.
To export Run-Logfiles to USB press
Save all Run-Logfiles to USB
or to export a specific Run-Logfile select a logfile from the list and press
Save Run-Logfile to USB
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85
in the Run-Logfile overview screen (see figure Figure 60). Use “Selected Run-Logfile to USB”
to save single Run-Logfiles to USB or “All Run-Logfiles to USB” to save all Run-Logfiles to
USB.
12.3.2 Power-On-Logfile
With each system start the Biometra TRIO Thermal Cycler performs an initial self-test and
stores the results in a Power-On-Logfile.
In the Power-On-Logfile screen the following information is listed:




Power-on by date and time
Last Power-on by date and time
Last Power-down by date and time
Table with failure messages by number, date, time, error code and message
Figure 63 Biometra TRIO Power-On-Logfile screen
12.3.3 Extended Self-Test-Logfile
During the Extended Self-Test the Biometra TRIO Thermal Cycler checks several functions
and components like cooler, thermal tracking, heating and cooling rate, refrigeration, heated
lid and regulation (see chapter 12.4). Press on the corresponding button to see the results for
a specific test summarized in tabular format.
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Figure 64 Biometra TRIO Extended Self-Test-Logfile screen
The test result can be “Passed” or “Failed” and is listed in the last row of each table for each
test.
12.3.4 Error-Logfile
The Biometra TRIO Thermal Cycler records all errors by time and date. The latest error is
shown in position in the Error Log table. Additionally the error code and error message are
listed.
Figure 65 Biometra TRIO Error-Logfile screen
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87
12.4 Extended Self-Test
During the Extended Self-Test the Biometra TRIO Thermal Cycler checks several functions
and components:
Test
Cooler
Function
Incubates the sample block to 4°C and checks, if the sample
block reaches 4°C and if the low temperature is hold for a longer
time.
Thermal tracking
Checks the synchronism of the control circuits and if they work
coordinated together.
Heating and cooling rate
Checks, if the specified average heating and cooling rate is
reached.
Refrigeration
The cooling sink and fans are checked to work properly together.
Gradient1
Checks, if the sample block reaches the set gradient
temperatures.
Heated lid
Tests, if the heated lid reaches the set temperature and if it holds
the temperature for a longer time.
Regulation
Tests, if the sample block is controlled properly.
1
not available for Biometra TRIO
In certain intervals the Biometra TRIO software will automatically recommend to execute the
Extended Self-Test. Although it is not necessary to perform the test it is highly
recommendable to follow the instructions and to let check the instrument itself.
Before starting the Extended Self-Test insert a microplate or, if applicable, two strips or a row
of tubes to the first and last column of the sample block (see figure Figure 66). It is very
important to load the block with plastic ware since during the Extended Self-Test the heated
lid is tested and this test can only be executed properly with plastic ware inserted. Also the
position of the plastic ware is important to ensure optimal pressure distribution and horizontal
levelling of the heated lid.
Figure 66 How to load plastic ware for the Extended Self-Test
If the plastic ware is properly inserted and the lid is closed the test can be started. To start
the Extended Self-Test press
Start Extended Self-Test
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Figure 67 Biometra TRIO Extended Self-Test screen
During the test the software provides a progress overview. If a test is already passed the
result “Failed” or “Passed” is displayed, for the currently performed test “Running” is shown
and all other tests are not finished yet.
Note: The Extended Self-Test will take approximately 30 minutes to finish and the instrument
is blocked, no other program can be started while the test is running. To stop the Extended
Self-Test press
Stop Extended Self-Test
If the test is stopped, no corresponding log file will be created.
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89
Figure 68 Biometra TRIO Extended Self-Test progress overview screen
The results of the Extended Self-Test are summarized and stored by the instrument and can
be viewed by the user (see chapter 12.3.3).
12.5 Service Info File (SINF)
The Service Info File is a helpful tool for remote failure diagnosis by the Biometra service
department.
Figure 69 Biometra TRIO create Service Info File screen
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To create a Service Info File connect a USB stick to the Biometra TRIO Thermal Cycler and
press
Info file to USB
The Service Info File can be send by e-mail to the Biometra service department. For contact
details see chapter 12.8.
12.6 Backup
The backup function is used to synchronize the memory contents of Biometra TRIO
instruments. By the backup function all folders, programs, users and user settings can be
stored to USB and transferred to another Biometra TRIO instrument. Connect a USB drive to
the instrument and press
Save backup file
to create a backup file. To load a backup file select a backup from the list
Figure 70 Biometra TRIO backup file screen
and press
Load backup file
Backup files that are no longer used can be deleted by using the functions
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Delete all Backup Files
Delete Backup File
12.7 Cycler Info
The Cycler Info Tool presents several general information about the instrument like:














Cycler type
Company
Serial number Cycler
Block type
Block serial number
Software version
Protocol version
Revision number power board
Software version power board
Software version power board-logic
Revision number processor board
Software version processor board
Revision number IO
Software version IO
The information is summarized in two screens. Press
System/Cycler Info
to toggle between the two screens.
Figure 71 Biometra TRIO cycler info screen
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12.8 Contact
Shows contact details for the Biometra service department.
Figure 72 Biometra TRIO contact details screen
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13 Adaptation of programs
If programs are exchanged between different thermal cycler models or of the sample block is
exchanged programs might need to be adapted prior to start or during editing. The following
table provides an overview for possible adaptations:
94
Cause
Example
Adjustment
Maximum gradient
range is exceeded.
A program for the 96 well
gradient silver block with a
programmed temperature
range > 30°C is to be
started on a 96 well
gradient aluminum block
and therefore exceeds the
block's maximum gradient
range.
The temperature range of the
gradient will be reduced based
on the average temperature of
the maximum permissible
value. If the average
temperature of the
programmed gradient is, for
example, 60°C and the
temperature range is ± 20°C,
it will be reduced to 60°C ±
15°C.
A program including a
gradient step is
transferred to a nongradient instrument.
The program is including a
gradient step started on a
block without gradient
function.
The gradient will be deleted
and the average
temperature/annealing
temperature will be used for
this step. If the average
temperature/annealing
temperature of the
programmed gradient is for
example 60°C, this value will
be used for the step.
Gradient out of limit
A program for the 96 well
silver block with a
programmed gradient
temperature below 20°C is
to be started on a 96 well
aluminum block and falls
below minimum permitted
temperature.
The lower temperature is
increased to the minimum
permitted value. If for example
a gradient is programmed
from 5°C to 25°C the gradient
is changed to 20°C to 25°C.
A program including a
temperature
optimization step
(TOS) is transferred
to an instrument that
does not offer this
function
A TOS program is
transferred from the
Biometra TRIO to another
thermal cycler
The mean value of the
programmed temperatures in
the TOS step for the left and
right sample block is used.
Instruction Manual TAdvanced TRIO 07/2015
The ramping rate
exceeds the limit
A program for the 96 well
gradient silver block is
started on a 96 well
aluminum block.
The ramp rate will be adjusted
to the maximum possible ramp
rate for the installed block
type. If the programmed ramp
rate is for example 8°C/s it will
be reduced to 6°C/s.
The ramping rate is
lower than the
maximum
A program for the 96 well
aluminum block is started
on a 96 well silver block.
The ramp rate will be adjusted
to the maximum possible ramp
rate for the installed block
type. If the programmed ramp
rate is for example 6°C/s it will
be increased to 8°C/s.
User defined ramping The ramping rate for a
rate
program step is lower than
maximum value
The user defined value is
maintained and does not
become adjusted
Note: If programs need to be adapted to the installed sample block prior to the start or
editing a message will be displayed. The user can confirm or can deny the adaptations. If
during program start the necessary adaptation is denied the program cannot be started by
the instrument.
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95
14 Short Manual
Safety Warnings
Before initial operation check the voltage selector configuration at the bottom to
ensure that the instrument has the correct voltage setting. Do not open the
instrument unless you are authorised to do so.
The thermoblock and the heated lid will reach high temperatures during
operation. Both thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil explosively. Always
wear safety goggles during operation. Close the lid before starting a program.
It is not necessary to apply oil into the opening of the block in order to improve
the heat transfer between the block and the sample tubes. If you still decide to
use oil, do not use silicon oil. Mineral oil may be used.
Ensure that both the rear and bottom ventilation slits at the rear and bottom of
the instrument are unobstructed. Insufficient ventilation can cause overheating
of the instrument. Tip: At regularly intervals clean the bottom's ventilation slits
from dust to ensure maximum venting capability.
Log in
After the Biometra TRIO has passed the power-on self-test the login screen is displayed:
 Press [Login] to log in as existing user
 Press [Quick start] for the non-user specific quick start of programs
 Press [EN/DE] to set the login screen language
Home Screen
After log in the Biometra TRIO home screen opens:
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Press [Quick start] for the user specific quick start of programs
Press [Programs] to edit, save, copy or run programs
Press [Incubate] to incubate the sample block at a constant temperature
Press [Tools] to access the system settings, user management, documentation, backup tool or
self-test functions
 Press [Logout] to log out




Edit, save and copy programs
Important note: It depends on your rights settings if you are allowed to create, delete, save, copy,
start and stop programs. If you do not have a specific right you will not have access to the
corresponding tools. Please ask your administrator or check your rights settings if you are missing a
button or function.
 Press [Programs] in the Biometra TRIO home screen. The program overview screen opens:
 To edit a program template press [New from template], select a template and press [Open
template].
 To edit a stored program select a user directory, select a program and press [Edit].
The Biometra TRIO programming screen opens. Use the following buttons to switch between the
different programming modes:
Graphical mode
Spreadsheet mode
Spreadsheet programming screen
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Graphical programming screen
97
 Define the program name. Touch the input box “Name” and enter a program name using the
Biometra TRIO keyboard.
 Define the heated lid status and temperature. Touch the input box “Lid” and switch the heated lid
on or off. If the heated lid is switched on enter a temperature between 30 °C and 110 °C.
 To switch heated lid preheating mode on or off press the corresponding button.
 Select a program step and press [Edit]. The edit step screen opens:
 Use the cursor keys at the top of the screen to switch between program steps. The number of the
currently activated step is displayed.
 Touch the input box “Temperature” and enter a temperature [°C]
 Touch the input box “Holdtime” and enter the time [h:mm:ss]. To program a pause step press the
button .
 To program loop touch the input box “Goto” and enter the step number the program shall go back
to. Then touch the input box “Cycles” and enter how often the loop will be repeated.
 To reduce or increase the annealing temperature from step to step within a loop press the button
∆T and set a negative or positive temperature increment.
 To increase or reduce the incubation time from step to step in a loop press the button ∆t and set a
time increment.
 To adjust the ramping rate press the button ∆R and enter any value between 0.1°C and max.
 To define a Temperature Optimisation Step press button TOS. Enter the primer annealing
temperature and define the temperature increment. The temperature for each sample block is
displayed. Press the button  to confirm the settings.
 To confirm all settings press the button  in the edit step screen. The software leaves the edit step
screen and shows programming screen in graphical or spreadsheet view.
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 To insert a step select a program step in the programming screen and press [Insert]. The edit step
screen opens. Enter the desired parameters and confirm the settings by pressing the button .
 To delete a step select a program step in the programming screen and press [Delete]. In the
security query confirm to delete the step by pressing the button .
Tip: Storing samples at 15°C rather than 4°C at the end of a run will increase the instruments life time.
 When the parameters for all steps are entered confirm the settings by pressing the button [Done]
in the programming screen. The software opens the screen to copy and save programs. Please
note that the program is not saved yet.
 To save the program select a user directory and a storage place, then press the button [Save].
Now the program is saved.
Save program
Copy/Delete programs
 To copy programs press the button [Programs] in the Biometra TRIO home screen.
 To copy all programs located in a user directory select a user directory and press the button [Copy
all]. Select the user directory the programs should be copied to and press the button [Save].
 To copy a single program select a user directory and a program and press the button [Copy].
Select a user directory, a storage place and press the button [Save].
 To delete all programs located in a user directory select a user directory, press the button [Delete
all] and confirm the security query.
 To delete a single program select a user directory and a program and press the button [Delete].
Confirm the security query to delete the selected program.
Start, pause and stop programs
 The Biometra TRIO thermal cycler stores the latest 5 edited or started programs for each user. For
quick start of a program press the [Quick start] button in the Biometra TRIO home screen. Select
a program from the list and press the [Start] button.
 To start a program from a user directory press the button [Programs] in the Biometra TRIO home
screen. In the next screen select a user directory and a program and press the [Start] button.
 After start the running program is shown in spreadsheet or graphical view. Use the same buttons
as for the programming screen to switch between the different modes.
 The current step is highlighted in yellow.
Spreadsheet view
Graphical view
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 To pause the active program press the button [Pause]. The color of the highlighted step changes
to blue and in the field “Step” the message Pause is shown.
 To continue a paused program press the button [Continue].
 To skip a step press the button [Skip]. The program will continue with the next step.
 To stop the active program press the button [Stop program] button and confirm the security query.
Programs with a pause in the last step have to be stopped manually as well.
Switch user management on or off (only for administrator)
 In the Biometra TRIO home screen press the button [Tools], in the next screen the button [UserManagement] and then use the checkboxes to switch the user management on or off.
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15 Troubleshooting
15.1 Forgotten password
Passwords can be changed by the systems administrator(s) and by users with the activated
right “Edit User” (see chapter 12.2.2). If the administrator Admin has forgotten the password
and no one else has the right “Edit user” use the function “Factory settings” (see chapter
12.1.7) to reset the system and load a backup file (see chapter 12.6) to restore it.
15.2 Slow heating and cooling
The Biometra TRIO is equipped with a strong ventilator for the cooling of the heat sink. The
inlet of this fan is located at the bottom side of the instrument. Make sure the inlet is not
clogged by dust or other material (e.g. a sheet of paper placed under the cycler can be
attached to the inlet as the fan is in operation). Dust can be removed easily from the inlet
using a conventional vacuum cleaner.
15.3 Auto restart
The Biometra TRIO Thermal Cycler features an automatic restart. If a power failure occurs
during the run the instrument will continue the run when the power returns. In case of longterm power failure (longer than 30 minutes) the instrument the instrument keeps the sample
block at 4°C (freeze step) and the user can decide to repeat the run with the same samples
or to discard them.
Note: After power failure the user has to log in again. In addition for a limited time information
can be displayed.
15.4 Auto restart due to unrecognized power failure
High voltage fluctuation can lead to an automatic restart of the Thermal Cycler. In this case
the cycler restarts at the step where there power failure has occurred. A message is
displayed showing (a) that a power failure occurred during the run and (b) when and at which
step the program was started again (see 15.3). To avoid voltage fluctuation, do not connect
the cycler to a socket shared by a strong power consumer like a refrigerator or a centrifuge.
Note: After power failure the user has to log in again. In addition for a limited time information
can be displayed.
15.5 Adaptation of protocols from other cyclers
Since the Biometra TRIO is a fast instrument it may be necessary to reduce the heating and
cooling ramps to run protocols from other cyclers. For the setting of the heating and cooling
ramps see chapter 8.6.4. Alternatively, the time settings may be extended.
15.6 Releasing wheel in case of blocked lid
When the lid is in the highest or lowest position, it may happen that the wheel is uncoupled.
In this situation the clutch mechanism is active in both directions (clicking noise in either
direction).
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101
To unlock wheel, press down metal pin with a ball pen and turn wheel carefully. This pin
overrides the automatic clutch mechanism. Thus, care must be taken not to apply excessive
pressure.
Release lid in upper position:
1) press pin
2) carefully turn wheel while holding the
pin down CLOCKWISE, until you feel
normal resistance (no more clicking
noise, clutch is released). Release pin
and turn lid down, until the clutch
mechanism is activated (clicking noise,
optimum pressure applied).
Release lid in down position:
1) press pin
2) carefully turn wheel while holding the
pin down COUNTER CLOCKWISE,
until you feel normal resistance (no
more clicking noise, clutch is released).
Release pin and turn wheel counter
clockwise until pressure is completely
released. Open lid.
Important: When the clutch mechanism is active (= optimum pressure is applied), do not use
the pin to further increase lid pressure, as this would lead to damage of tubes and
instrument!
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16 Maintenance and Repair
16.1 Factory calibration
This instrument is supplied fully calibrated by the manufacturer before delivery, using
equipment traceable to national standards. The Biometra Quality Management- System is
certified by BSI for BS EN ISO 9001:2008 and the used measurement system is tested by an
accredited testing laboratory in accordance with EN ISO 17025 at prescribed intervals and
gets regularly calibrated. If the instrument is installed in an environment that meets the
requested working conditions (see chapter 2.5), a temperature verification or calibration after
installation is not required.
16.2 Cleaning and Maintenance
Depending upon the installation site the Biometra TRIO Thermal Cycler should be cleaned
from time to time to maintain maximum performance. Occasionally cleaning of the air inlet
contributes to maintain the instruments efficiency as insufficient airflow will lead to reduced
heating and cooling rates (see chapter 15.1). The air inflow is located at the bottom side of
the instrument. Be sure that the inlet is not clogged by dust or other material (e.g. a sheet of
paper or a plastic bag). Dust can be removed easily from the inlet with a conventional
vacuum cleaner. Additionally, the Thermal Cycler housing may be cleaned from time to time
with a smooth cotton cloth. Do not use strong detergents, organic solvents or abrasive
agents to clean the instrument and the sample block!
Important: Appropriate safety regulations must be observed when working with infectious or
pathogenic material.
16.3 Servicing and Repair
The Biometra TRIO Thermal Cycler contains no user serviceable parts. Do not open the
housing instrument. Service and repair may only be carried out by the Biometra service
department or otherwise qualified technical personal.
Dependent on the usage the Biometra TRIO thermal cycler automatically shows a message
screen recommending a preventive maintenance. Furthermore the Biometra service
department offers maintenances and temperature verifications. Biometra recommends an
annual maintenance and a biannual temperature check for all thermal cyclers. Please call the
following phone number for detailed information: +49 551-50881-10/12.
16.4 Firmware update
For instruction for firmware upgrade, please contact the Biometra service department or your
local distributor/sales representative.
16.5 Replacement of spare parts
Only original spare parts mentioned in these operating instructions are allowed.
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103
17 Service
Should you have any problems with this unit, please contact our service department or your
local Biometra dealer:
Biometra GmbH
Service Department
Rudolf-Wissell-Straße 14 - 16
D-37079 Göttingen
Phone:++49 (0)5 51 50 68 6 - 10 or 12
Fax:
e-mail:
++49 (0)5 51 50 68 6 -11
[email protected]
Note: If you would like to send the unit back to us, please read the following return
instructions in chapter 17.1.
17.1 Instructions for return shipment
In case of an instrument failure that cannot be fixed by the procedures described in section
13 please proceed as follows:

Return only defective devices. For technical problems which are not definitively
recognisable as device faults please contact the technical service department at Biometra
(Tel.: +49 551-50881-10/12, Fax: +49 551-50881-11, e-mail: [email protected]).

Please contact our service department for providing a return authorization number
(RAN). This number has to be applied clearly visible to the outer box. Returns without
the RAN will be not be accepted!

Important: Carefully clean all parts of the instrument of biologically dangerous, chemical or
radioactive contaminants. If an instrument is contaminated, Biometra will be forced to
refuse to accept the device. The sender of the repair order will be held liable for possible
losses resulting from insufficient decontamination of the device.

Please prepare written confirmation that the device is free from biologically dangerous and
radioactive contaminants. The declaration of decontamination (see section 18) must be
attached to the outside of the packaging.

Use the original packing material. If not available, contact Biometra or your local
distributor.

Label the outside of the box with “CAUTION! SENSITIVE ELECTRONIC INSTRUMENT!”

Please enclose a note which contains the following:
a)
Sender’s name and address,
b)
Name of a contact person for further inquiries with telephone number,
c)
Description of the fault, which also reveals during which procedures the fault
occurred, if possible
17.2 Packing of the Biometra TRIO Thermal Cycler
Biometra uses an extra designed packaging system with inserts of foamed material.
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Note: The Thermal Cycler is only protected from transport damage if the original transport
box is used and the packing instructions are followed. Biometra is not responsible for
transport damage by improper packing.
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105
18 Equipment Decontamination Certificate
To enable us to comply with German law (i.e. §71 StrlSchV, §17 GefStoffV and §19 ChemG)
and to avoid exposure to hazardous materials during handling or repair, please complete this
form, prior to the equipment leaving your laboratory.
COMPANY / INSTITUTE
__________________________________________________
ADDRESS
______________________________________________________________
PHONE NO
_________________________
E-MAIL
______________________________________________________________
EQUIPMENT
FAX NO_________________________
Model
Serial No
______________
______________
______________
______________
______________
______________
______________
______________
If on loan / evaluation Start Date: __________________
Finish Date __________________
Hazardous materials used with this equipment:
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
Method of cleaning / decontamination:
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
The equipment has been cleaned and decontaminated:
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Instruction Manual TAdvanced TRIO 07/2015
NAME __________________________________ POSITION _______________________
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
SIGNED ________________________________ DATE ____________________________
PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA
DISTRIBUTOR TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS
WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
General Information for Decontamination:
Please contact your responsible health & safety officer for details.
Use of radioactive substances:
Please contact your responsible person for details.
Use of genetically change organism or parts of those:
Please contact your responsible person for details.
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107
19 Note for the disposal of electric / electronic waste.
Note
for disposal of electric / electronic waste
Hinweis
für die Entsorgung von Elektroaltgeräten
Renseignement
du traitement des déchets des appareils
électrique / électronique
This symbol (the crossed-out wheelie bin) means, that this product should be brought to the return and / or
separate systems available to end-users according to yours country regulations, when this product has reached
the end of its lifetime.
For details, please contact your local distributor!
This symbol applies only to the countries within the EEA*.
EEA = European Economics Area, comprising all EU-members plus Norway, Iceland and Liechtenstein.
------------------------------------------------------------------------------------------------------------------------------------------------------Dieses Symbol (die durchgestrichene Abfalltonne) bedeutet, dass dieses Produkt von der Firma Biometra
für eine kostenlose Entsorgung zurückgenommen wird. Dies gilt nur für Geräte, die innerhalb
Deutschlands gekauft worden sind.
Kontaktieren Sie für die Entsorgung bitte die Biometra Service-Abteilung!
Außerhalb Deutschlands wenden Sie sich bitte an den lokalen Händler.
Dieses Symbol gilt nur in Staaten des EWR*.
*EWR = Europäischer Wirtschaftsraum, umfasst die EU-Mitgliedsstaaten sowie Norwegen, Island und
Liechtenstein.
------------------------------------------------------------------------------------------------------------------------------------------------------Cet symbol (conteneur à déchets barré d´une croix) signifie que le produit, en fin de vie, doit être retourné
à un des systèmes de collecte mis à la disposition des utilisateurs finaux en conséquence des
régulations par la loi de votre pays.
Pour des information additionel nous Vous demandons de contacter votre distributeur!
Cet symbole s´ápplique uniquement aux pays de l´EEE*.
EEE = Espace économique européen, qui regroupe les États membres de l´UE et la Norvège, Islande et le
Liechtenstein.
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20 Declaration of Conformity
EU – Konformitätserklärung
EC - Declaration of Conformity
Göttingen, August 2015
im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung innerhalb
bestimmter Spannungsgrenzen 2006/95/EG
following the EC directive about electrical equipment for use within certain limits of voltage
2006/95/EC
und / and
im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit 2004/108/EG.
following the EC directive about the electromagnetic compatibility 2004/108/EC .
Hiermit erklären wir, dass folgende Thermocycler:
Herewith we declare that the following Thermocyclers:
Typen / types:
Biometra TRIO 30, Biometra TRIO 48, Biometra combi
Best.-Nr. / Order No.:
846-x-070-720, 846-x-070-723, 846-x-070-724
x = 2 für / for 230 V, 4 für / for 115 V, 5 für / for 110V
den grundlegenden Anforderungen der
corresponds to the basic requirements of
EG-Niederspannungsrichtlinie 2006/95/EG und der
EC low voltage directive 2006/95/EC and the
EG-Richtlinie über die elektromagnetische Verträglichkeit 2004/108/EG entsprechen.
EC directive about the electromagnetic compatibility 2004/108/EC .
Dr. Juergen Otte
Head of Quality Management
For and behalf of Biometra GmbH
Certificate number FS 68563
Instruction Manual TAdvanced TRIO 07/2015
109
21 Warranty
This Biometra instrument has been carefully build, inspected and quality controlled before
dispatch. Hereby Biometra warrants that this instrument conforms to the specifications given
in this manual. This warranty covers defects in materials or workmanship as described under
the following conditions:
This warranty is valid for 24 month from date of shipment to the customer from Biometra.
This warranty will not be extended to a third party without a written agreement of Biometra.
This warranty covers only the instrument and all original accessories delivered with the
instrument. This warranty is valid only if the instrument is operated as described in the
manual.
Biometra will repair or replace each part which is returned and found to be defective. This
warranty does not apply to wear from normal use, failure to follow operating instructions,
negligence or to parts altered or abused.
110
Instruction Manual TAdvanced TRIO 07/2015
22
Subject Index
Autorestart .................................................101
Backup..........................................................91
Block status .................................................31
Declaration of Conformity ........................109
Decontamination Certificate ....................106
Display
Run.............................................................55
Disposal......................................................108
Documentation ............................................82
Error-Logfile ...............................................87
Extended Self-Test .....................................86
Power-On-Logfile.......................................86
Run-Logfile.................................................83
Firmware update .......................................103
Heated lid .....................................................20
Heated Lid..................................................101
Home Screen...............................................31
Incubation ....................................................35
Instrument
Name .........................................................72
Log in ............................................................29
Log in Screen ..............................................28
Maintenance ..............................................103
Operation Voltage .......................................18
Packing .......................................................104
Password
Administrator ............................................30
Forgotten ................................................101
Power On Self-Test ....................................28
Program
Adaptation .................................................94
Continue ....................................................57
Copy ...........................................................51
Copy all ......................................................52
Cycles .......................................................43
Delete ........................................................53
Delete all ..................................................54
Edit step ...................................................42
Graphical programming .........................37
Heated lid temperature ..........................41
Name ........................................................41
New ...........................................................39
Pause .........................................................56
Preheating mode.....................................41
Preview ......................................................50
Ramp rate ................................................45
Save ..........................................................47
Spreadsheet programming ....................37
Start ...........................................................49
Instruction Manual TAdvanced TRIO 07/2015
Stop ........................................................... 57
Temperature increment ......................... 44
Temperature Optimisation Step (TOS)45
Template ................................................... 39
Time increment ....................................... 44
Protocol Wizard .......................................... 59
Quick start function .................................... 32
Repair ........................................................ 103
Safety precautions ..................................... 14
Servicing .................................................... 103
Settings ........................................................ 68
Automatic user logout .............................. 70
Beeper....................................................... 71
Date and Time........................................... 69
Display brightness ..................................... 73
Factory settings......................................... 75
Network .................................................... 72
Network Settings ...................................... 72
Network User ............................................ 73
Touch screen calibration .......................... 74
User Management .................................... 76
Short Manual .............................................. 96
Skip
Step ........................................................... 57
Software ...................................................... 21
Cancel entry .............................................. 23
Commands and icons................................ 23
Confirm entry............................................ 23
Cursor keys ............................................... 22
Keyboard ................................................. 21
Keypad ..................................................... 22
Technical specifications ............................ 11
Tools ............................................................ 68
Contact...................................................... 93
Cycler Info ................................................. 92
Extended Self-Test .................................... 88
Service Info File ......................................... 90
Touch screen
Calibration ................................................ 74
User Management ..................................... 76
Create User ............................................. 78
Delete User ............................................. 82
Edit User .................................................. 79
Switch on or off ....................................... 76
User Properties ...................................... 80
User Rights ............................................. 80
Warranty .................................................... 110
111
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