B4472 Porvair Sciences catalogue 2010

B4472 Porvair Sciences catalogue 2010
C ATA LO G U E O F M I C R O P L AT E S & M I C R O P L AT E E Q U I P M E N T
FO U RTH ED ITI O N
STORAGE/COLLECTION
FOREWORD
Welcome to the 2010/2011 edition of the Porvair Sciences
Microplate Catalogue.
Many scientists around the world have come to rely upon
previous editions of the Microplate Catalogue as a primary
source of information that enables them to choose the right
assay, filter, storage or PCR plate.
LIFE SCIENCES
This latest edition brings together information on Porvair
Sciences comprehensive microplate and microplate
instruments product range. Reflecting our commitment to
continuous microplate technology innovation, the 2010/2011
catalogue introduces a suite of exciting new products.
SOLID PHASE EXTRACTION
For example, there’s our new Glass Vial Storage Plate – 96
high quality glass vials in a sealable 44mm storage block,
ideal for UHPLC applications. Our instrument range boasts
two new thermal plate sealers. MicroSeal is a budget priced
heat sealer with great performance, whilst our popular
MiniSeal gets a makeover to become MiniSeal Plus – quicker,
quieter, safer and more economical than before. Add to this
new PCR plates, more sterile plate options and a fantastic
new range of seals and you will see that the 2010/2011
catalogue remains an indispensable reference guide to any
laboratory using, or interested in using, microplates.
EQUIPMENT
Porvair Sciences is one of the largest global manufacturers of
ultra-clean microplates for life science, synthetic chemistry
and many other applications. Our modern Class V clean room
facility in Wrexham, UK is responsible for production of all of
our clear-bottom plate products, including the highlyacclaimed glass bottom Krystal plates. The same highly
experienced UK team also provides first-class customer service
to our customers and distributors worldwide. Should you be
interested in evaluating and testing any of our microplates just give our friendly team a call, or send them an email
asking for a free sample pack of your microplate of choice.
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
With a proud history of innovative microplate manufacturing
dating back to 1992, Porvair Sciences’ mission is to become
your preferred global partner for microplate products. Our
technical and sales teams are at your disposal – so take a look
through this catalogue and you will see a wide array of
microplate products and instruments designed to enable you
to get better results faster and more consistently.
Porvair Sciences products are designed for use in research environments and are not
suitable for clinical, diagnostic or medical use
All trademarks are acknowledged.
Varian® is a registered trade mark of the Varian Corporation Inc.
Polymerase Chain Reaction (PCR) is a process covered by patents owned by HoffmannLa Roche.
Scorpion™ is a trademark of K Biosystems Ltd.
SealPlate™, ThermalSeal RT2, RT5, RT2RR™, Texan™, AbsorpMax™, AeraSeal™,
AlumaSeal™, EZ-Pierce™ and X-Pierce™ are trademarks of Excel Scientific Inc.
Thermobond II ® is a registered trademark of Porvair Sciences Ltd. MicroLute™,
MaxiLute™, TriSeal™,
Ultravap™, MiniSeal™, MiniVap™, MicroStream™, Krystal™ and BioVyon™ are all
trademarks of Porvair Plc.
Megabace™ is a registered trademark of Applied Biosystems.
Specifications subject to change without notice. E&OE.
2
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION
CONTENTS
Contents
Page 13
Page 18
Page 20
Page 24
Page 28
Page 30
Page 31
Page 35
Solid phase extraction
MicroLute
Combinatorial MicroLute
MaxiLute
P 3 Protein Precipitation Plates
SPE starter pack
BioVyon™ co-sinter products
Page 37
Page 38
Page 39
Page 40
Page 41
Page 42
Equipment
Vacuum manifolds
MiniVap microplate evaporator
UltraVap microplate evaporator
MicroSeal manual thermal plate sealer
MiniSeal semi-automatic sealer
TriSeal automated plate sealer
Mat Capper and De-capper
Adhesive-seal selection guide
Seals and foils
MicroStream thawing station
MicroShake orbital shaker
Page 43
Page 45
Page 46
Page 48
Page 49
Page 50
Page 51
Page 52
Page 53
Page 57
Page 57
Automation accessories
Reservoir trays
Disposable reservoir trays
Page 58
Page 62
Custom manufacture
Page 64
Index by number
Page 65
Chemical compatibility chart
Page 67
SOLID PHASE EXTRACTION
Life Sciences
PCR plates – polypropylene
Choosing the correct assay plate
Solid bottom assay plates
Clear bottom assay plates
Glass bottom assay plates
UV clear bottom assay plates
Filtration plates
Bacti-growth plates
EQUIPMENT
Page 6
Page 7
Page 8
Page 9
Page 9
Page 10
Page 11
AUTOM ATION ACCESSORIES
Storage/Collection
96-well deep square
96-well deep round
96-well shallow round
96-well round low profile
96-well glass vial storage plate
384-well
Large volume
LIFE SCIENCES
Page 4
W W W. M I C R O P L A T E S . C O M
3
CUSTOM M ANUFACTURE
Application table
STORAGE/COLLECTION
A P P L I C AT I O N TA B L E
Application table
CL
UG
UN
ER
E
Y
G
AG
IN
■
OV
OR
SC
ST
DI
D
EN
■
RE
■
PO
■
S
■
DR
M
SC
■
IC
LIFE SCIENCES
CO
S
IS
■
M
YS
AL
G
IN
■
EO
AN
ST
G
S
Y
GY
IN
LO
EN
TE
RE
NO
OT
L
PR
TA
S/
HT
IC
EN
OD
SC
CH
IE
TR
UD
IS
■
M
NM
NO
RO
E
FO
PL
TE
ST
EM
■
GE
VI
M
Y
TH
CH
SOLID PHASE EXTRACTION
EN
SA
RA
AL
OW
RI
GR
TO
AR
NA
AL
BI
IC
M
IN
CO
Storage/collection
■
■
Life science
■
Solid bottom
■
■
■
Glass bottom
■
■
■
■
■
Quartz bottom
■
■
■
■
■
Clear bottom
■
■
PCR plates
■
Filtration plates
■
■
■
■
■
Bacti-growth plates
SPE
MicroLute
■
■
MaxiLute
EQUIPMENT
CombiLute
■
■
Equipment
AUTOM ATION ACCESSORIES
CUSTOM M ANUFACTURE
■
P3
Manifolds
■
■
■
■
Evaporators
■
■
■
■
Cappers
■
■
■
■
■
Sealers
■
■
■
■
■
■
■
Seals
■
■
■
■
■
■
Thawing station
■
■
■
■
■
■
■
■
Automation
Reservoir trays
4
W W W. M I C R O P L A T E S . C O M
■
■
■
STORAGE/COLLECTION
Are your plates whiter than white?
Or do they harbour a dirty secret?
Ramp
30°C/min
Initial temp 70°C Hold 2 min
Final temp 310°C Hold 5 min
EQUIPMENT
Porvair
Results from the GC-MS showed good performance from the
Porvair microplate, with a clean caffeine peak and no
significant levels of leachable or extractable compounds. Plate
B shown here, is a typical example of the other commercial
microplates tested, which included two major German
manufacturers and several other well-known suppliers. These
variously displayed extra peaks with a variety of retention
times across the run, indicating several different
contaminants. Further analysis of these extra peaks revealed
that they were, indeed, mostly recognized chemicals used as
additives in the polypropylene to assist the injection moulding
process.
AUTOM ATION ACCESSORIES
Porvair Sciences has carried out tests on many manufacturers’
deep well plates to establish levels of extractables in each. A
typical example is illustrated. Extractables testing was
performed by adding a volume of methanol, sufficient to half
fill the wells to each test plate. An internal standard
comprising methanol spiked with 10 mg/ml of caffeine was
used to give a consistent peak. Samples were taken from each
well and subjected to analysis on a GC-MS system with
splitless injection @ 250°C, using an HP - 5MS capillary
column. A temperature gradient was programmed such that:
800000
700000
600000
500000
Abundance
400000
300000
200000
14.8
14.5
14.1
13.8
13.5
13.2
12
12.9
12.6
12.3
11
11.6
11.3
10.7
10.4
10.1
9.77
9.46
9.15
7.9
8.84
8.52
8.21
7.59
7.27
6.96
6.65
5.4
6.34
6.02
5.71
0
5.09
100000
Time
Plate B
800000
700000
600000
500000
Abundance
400000
LIFE SCIENCES
This allows rapid production of plates and lower costs, but
may compromise the integrity of samples or compounds
which are stored in such plates for extended periods. The
problem is particularly acute where compounds are stored as
solutions in solvents such as methanol or DMSO, as these
excellent solvents have a tendency to extract from the
polypropylene base material any added extractable
compounds such as mould release agents or polymer flow
improvers.
5
SOLID PHASE EXTRACTION
Deep well polypropylene microplates are commonly used for
sample storage in life science laboratories. An essential aspect
of the manufacture of these plates is the selection of clean
raw materials for injection moulding. Polypropylene is an inert
and heat resistant material, ideal for sample storage.
However, all grades of polypropylene are not the same, for
example, Injection moulding grades of polypropylene often
have high concentrations of chemicals to aid the moulding
process.
200000
100000
Time
14.5
14.1
13.8
13.5
13.2
12.9
12
12.6
12.3
11.6
11.3
11
10.7
10.4
10.1
9.77
9.46
9.15
8.84
8.52
8.21
7.9
7.59
7.27
6.96
6.65
6.34
6.02
5.4
5.71
5.09
0
From this it can be seen that it is essential to select the correct
grade of polypropylene material for storage plate
manufacture. The full report can be downloaded from our
website.
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
300000
STO R A G E / C O L L E C T I O N – 9 6 -W E L L D E E P S Q UA R E
96-well deep square
44mm height
●
Made from virgin polypropylene
●
Tested for low extractables
●
Tight specification levels
●
V bottom to allow total liquid
removal, partial collection and to
aid re-suspension
●
No inner edges to allow better
collection of magnetic beads
●
Manufactured under DNase/ RNase
free environment
●
Working volumes of 350µl, 1ml
and 2ml
●
Raised well rims to improve heat
sealing
●
Conical base which aids sample
concentration, reconstitution and
centrifugation
●
Sterile and non-sterile versions
available
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
LIFE SCIENCES
The family of 96 square well plates was
designed so that it would make the interchange
of plates simpler in automated systems. All
three plates have the same geometry and the
only variable is the well depth.
SOLID PHASE EXTRACTION
6
Storage plates square
Working well volume
Sterile
Use cap mat
Quantity/case
Cat. no.
350µl
–
219004
50
219006
350µl
✓
219019
50
219025
1ml
–
219004
50
219008
1ml
✓
219019
50
219026
2ml
–
219004
50
219009
2ml
✓
219019
50
219027
W W W. M I C R O P L A T E S . C O M
STO R A G E / C O L L E C T I O N – 9 6 -W E L L D E E P RO U N D
96-well deep round
42mm height
7
●
Alphanumeric grid-referencing
●
DNase/ RNase free
●
Packed in sealed sleeves of 5 plates
●
Rimmed version to stop cross
contamination and enable a better
seal
●
Non rimmed version to allow
insertion of vials, or where the seal
is not crucial
●
Cylindrical well with round bottom
for optimal mixing and recovery
●
Very easy to use with automated
sample handling systems
●
Can be stored at -80ºC
SOLID PHASE EXTRACTION
Manufactured from pre-tested
polypropylene for low extractables
NO RIM
Polypropylene storage plates round
Rim and bottom well shape
Colour
Well volume
Sterile
Use cap mat
Quantity/case
Cat. no.
Raised- round
Natural
1ml
–
219036
50
219002
Raised- round
Natural
1ml
✓
219042
50
219012
No rim- round
Natural
1ml
–
219036
50
219037
Raised- round
Blue
1ml
–
219036
50
219432
Raised- round
Red
1ml
–
219036
50
219422
Raised- round
Black
1ml
–
219036
50
219412
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
WITH RIM
AUTOM ATION ACCESSORIES
EQUIPMENT
●
LIFE SCIENCES
The plates are made in virgin polypropylene
to minimise extractables. Working volume is
1ml per well and total volume is 1.1ml.
Coloured plates aid identification when
retrieving from storage. Black plates are
suitable for storing light sensitive compounds.
STO R A G E / C O L L E C T I O N – 9 6 -W E L L S H A L LOW RO U N D
96-well shallow round
14.7mm height
Porvair Sciences has developed a number of
storage/collection plates to help in the
fields of cell biology, molecular biology, drug
discovery, combinatorial chemistry, screening
and genomics. The plates are manufactured
under clean room conditions and a significant
number are DNase/RNase free. They are
mainly made from polypropylene, an inert
material giving heat and solvent resistant
qualities. Porvair Sciences offers the largest
combination of well shape, number of wells
and well volume on the market. Each is made
to the SBS/ANSI format for compatibility with
most readers/washers and automated
equipment.
Porvair Sciences offers three plates with well
capacities of 350µl, 270µl and 220µl. They
have flat-, round- or V-bottoms and can be
used for compound storage and culturing.
●
Manufactured in polypropylene
●
Alphanumeric grid reference
●
Round and V-bottom allow greater
liquid removal and particulate
collection
●
Raised rims improve sealing and
stop cross contamination
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
LIFE SCIENCES
8
ALL DIMENSIONS IN MM
Storage plates round
CUSTOM M ANUFACTURE
Rim and bottom well shape
Material
Well volume
Sterile
Quantity/case
Cat. no.
No rim, flat
Polypropylene
350µl
–
100
208003
Raised-round
Polypropylene
270µl
–
100
209003
Raised-V
Polypropylene
220µl
–
100
210003
W W W. M I C R O P L A T E S . C O M
STO R A G E / C O L L E C T I O N – 9 6 -W E L L RO U N D LOW P RO F I L E
96-well round low profile
A new introduction to the Porvair range is a low
profile 1.1ml 96-well round plate with a height of
just 27mm. This allows more plates to be stored or
stacked in a given space. The plates are made with
extractable-free polypropylene for excellent results.
A specific anti-evaporation cap mat is also available
for this plate manufactured from thermoplastic
elastomer.
●
Minimises space needed for
compound storage
●
Pure virgin polypropylene
●
Matching snug-fitting cap mat
LIFE SCIENCES
Allows stacking in higher densities
CAP MAT
Dimensioned drawing available on request.
96-well round low profile
Description
96-well round low profile plate
Thermoplastic elastomer cap mat for low profile plate
Working well vol
Sterile
Qty/case
Cat. no.
1ml
–
50
219250
–
–
100
219251
96-well glass vial
storage plate
SOLID PHASE EXTRACTION
●
9
44mm height
Store aggressive solvents in glass vials
●
Chemically resistant
●
No trace impurities for UHPLC
●
Prevent evaporation with matching
cap mat
AUTOM ATION ACCESSORIES
●
EQUIPMENT
The new Porvair glass vial storage plate combines
96 borosilicate glass vials of 700µl into a rigid
polypropylene carrier plate for ease of storage and
transportation. The glass vials are round bottomed
and the plate can be capped with our matching
square-well cap mat to prevent evaporation or
contamination. This is the zero-leachates solution
for UHPLC and where organic solvents must be
stored in a plate footprint.
Description
Qty/case
Cat. no.
96-well glass vial storage plate
12
229230
Square well silicone cap mat for 229230
5
229091
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
96-well glass vial storage plate
STO R A G E / C O L L E C T I O N – 3 8 4 -W E L L
384-well
●
Manufactured for high density
sample collection / storage
●
Two sizes: 58µl and 300µl per well
working volume
●
Each are designed to allow almost
total removal of liquid sample
●
Extra flat – allows plate sealing
●
Storage temperature down to –80ºC
●
Available sterile and non sterile
●
All manufactured to SBS/ANSI
specifications
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
LIFE SCIENCES
10
Porvair Sciences has a selection of 384-well
polypropylene plates to suit most applications.
All of them are made from pre-tested
polypropylene in Class 100000 clean rooms.
Storage plates 384-well
CUSTOM M ANUFACTURE
Well shape, top & bottom
Working well vol
Sterile
Qty/case
Cat. no.
Square-round
58µl
–
60
224001
Square-V
300µl
–
48
219040
Square-V
300µl
✓
48
219041
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION – LARGE VOLUME
Large volume
SOLID PHASE EXTRACTION
24-well features:
● 24 wells with a working capacity of 10ml/well
● Standard height (44mm) of a deep well plate
● Sterile or non sterile versions
● Lidded version available
48-well features:
●
●
●
11
LIFE SCIENCES
The range of large volume plates is designed to meet special
requirements. Focused on the combinatorial, environmental
and food technology markets, it allows large volumes of
samples to be transported in recognised SBS/ANSI format
microplates, to allow greater automation. Plates will accept
the Porvair universal lid (see page 56). All plates are
manufactured from virgin polypropylene, can be heat sealed
and stored for prolonged periods at -80ºC.
Two versions:
5ml/well, 44mm high
7ml/well, 68mm high
No of wells
Working well vol
Sterile
Lid
Qty/ case
Cat. no.
Rectangle well with a V bottom
24
10ml
–
–
25
360013
Rectangle, V bottom (bulk pack)
24
10ml
✓
–
25
360115
Rectangle well with a V bottom
24
10ml
–
✓
25
360077
Rectangle, V bottom (single pack)
24
10ml
✓
✓
25
360079
Rectangle, V bottom (with bar code)
24
10ml
✓
✓
25
360080
Rectangle well with a V bottom
48
5ml
–
25
360002
Rectangle well with a V bottom
48
7ml
–
30
360004
Centrifuge support plate
The support plate is designed to fit into the
skirt of the deep well plates. This then allows
the plate to be used in a centrifuge without
the risk of the plate distorting and causing the
well to leak.
●
●
Available for the 2ml and 1ml square deep well
plates
Easily fitted below plates
AUTOM ATION ACCESSORIES
Well shape, top
EQUIPMENT
High volume deep well microplates
Description
Quantity/pack size
Cat. no.
Centrifuge support plates for 219008 & 219026
2
228018
Centrifuge support plates for 219009 & 219027
2
228019
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
Deep well microplate accessories
STORAGE/COLLECTION – LARGE VOLUME
SOLID PHASE EXTRACTION
LIFE SCIENCES
12
360013
360002
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
CENTRIFUGE SUPPORT PLATE
360004
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION
L I F E S C I E N C E S – P C R P L AT E S P O LY P R O P Y L E N E
PCR plates –
polypropylene
Porvair premium PCR plates are made from
polypropylene for extra rigidity. These plates
are compatible with the majority of 96- and
384-well block PCR and sequencing
instruments, including FAST sequencers. Our
expanded range includes traditional no-skirt,
half-skirt and fully skirted plates with standard
350µl wells and in addition 200µl Low Profile
versions of these. The latest ‘elevated’ or
‘chimney’ well plates are also catered for with
several new products. To ensure full
compatibility with robotic systems, these new
DNase, RNase- and pyrogen-free thermal
cycler plates feature high rigidity to minimise
distortion before and after thermal cycling.
DNA and DNAase/RNAase free
●
Non-pyrogenic
●
Range designed to fit a variety of popular thermal
cyclers and sequencers
●
Raised rim for maximum sealing surface
●
Thin wall design for better heat transfer
●
96-well have 350µl or 200µl capacity
●
384-well have a 50µl capacity
●
All plates conform to ANSI/SBS specification
PCR plates – polypropylene
Plate description
Qty/pack
Cat. no.
96-well PCR microplate, Polypropylene, DNase, RNase free, No skirt, Standard profile
100
286101
96-well PCR microplate, Polypropylene, DNase, RNase free, No skirt, Low profile
100
286102
96-well PCR microplate, Polypropylene, DNase, RNase free, No skirt, Standard profile, Elevated
100
286103
96-well PCR microplate, Polypropylene, DNase, RNase free, Half skirt, Low profile
100
286104
96-well PCR microplate, Polypropylene, DNase, RNase free, Half skirt, Standard profile
100
286105
96-well PCR microplate, Polypropylene, DNase, RNase free, Half skirt, Standard profile, Elevated
100
286106
96-well PCR microplate, Polypropylene, DNase, RNase free, Semi-skirt, Low profile, Elevated
100
286107
96-well PCR microplate, Polypropylene, DNase, RNase free, Full skirt, Low profile
100
286108
96-well PCR microplate, Polypropylene, DNase, RNase free, Full skirt, Low Profile, Megabace™
100
286109
96-well PCR microplate, Polypropylene, DNase, RNase free, Half skirt, Straight sided, Standard profile
100
286110
384-well PCR microplate, Polypropylene, DNase, RNase free, Full skirt, 50µl per well
100
286111
96-well PCR microplate, Polypropylene, White, DNase, RNase free, Roche Lightcycler, RTPCR
100
286112
384-well PCR microplate, Polypropylene, White, DNase, RNase free, Roche Lightcycler, RTPCR
100
286115
W W W. M I C R O P L A T E S . C O M
SOLID PHASE EXTRACTION
●
EQUIPMENT
Prominent black printed axis
AUTOM ATION ACCESSORIES
Polycarbonate plates remain available; please
contact your distributor or Porvair Sciences.
●
CUSTOM M ANUFACTURE
A comprehensive PCR plate selection guide
can be found on pages 14 and 15. If you
cannot see your sequencer in the list, please
contact our Customer Service team at
[email protected] who will be
pleased to advise you of the most suitable
plate for your application and equipment.
13
Thermal cycler compatibility guide
15
61 E
28 HIT
L W 12
1
EL
W 86 ITE
2
H
4W 1
38
1
RT
KI 61 L
I S 28 WEL
M
40
SE
38 611 T
28 SKIR
I
M 08
SE 861 ED
2 IRT
SK 07
61 T
28 SKIR
I
M MI
SE SE GE
06 D
61 D E
28 ISE 105 T
RA 286 KIR
IS
IRT
M 04
SK
SE 61 T
28 SKIR
I
M 03
SE 61 P
28 Y TO
NE 02
IM 61 D
CH 28 IRTE
1
SK
UN 610 D
28 IRTE
SK
UN
STORAGE/COLLECTION
L I F E S C I E N C E S – P C R P L AT E S P O LY P R O P Y L E N E
96-WELL UNLESS STATED
Well shape
Standard well (overall height approx. 21mm,
max. capacity > 350µl)
Low profile well (overall height approx. 16mm,
max. capacity approx. 200µl)
14
Manufacturer
SOLID PHASE EXTRACTION
Applied Biosystems
Thermal cyclers
‘Real time’ cyclers
Sequencers
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
GE / Amersham
Sequencers
Beckman
Sequencers
Biometra
Thermal cyclers
Bio-Rad/MJ Research
Thermal cyclers
‘Real time’ cyclers
Sequencers
■
■
■
■
■
■
■
■
■
50µl
Thermal cycler model
2700
9600
9700
9800 ‘Fast’
Veriti 0.1ml
Veriti 0.2ml
Veriti 384
5700
PRISM 7000
7300
7500
7500 ‘Fast’
7700
7900HT
STEP ONE
STEP ONE PLUS
PRISM 310
PRISM 3100
3130(XL)
3700
PRISM 3730 (XL)
■
■
■
■
■
■
■
■
■
■
■
■
■
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CEQ
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Uno
Uno II
T1 Thermal Cycler
Tgradient
Trobot
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Gene Cycler
PTC-100
PTC-200
PTC-225 TETRAD
Dyad/Dyad Disciple
iCYCLER
MyCycler
Mini Gradient
Personal
DNA Engine family
C1000/S1000
Opticon/Opticon 2
Chromo-4
iCYCLER
MyiQ
iQ5
CFX96
CFX384
BaseStation
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MegaBACE 1000
MegaBACE 4000
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61 E
28 HIT
L W 12
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W 86 ITE
2
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4W 1
38
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KI 61 L
I S 28 EL
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M
40
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38 611 T
28 SKIR
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M 08
SE 861 ED
2 IRT
SK 07
61 T
28 SKIR
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SE SEMGE
06 D
61 D E 5
28 ISE 610 T
RA 28 KIR
IS 4
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SK
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S
28 SKIR
I
M 03
SE 61 P
28 Y TO
NE 02
IM 61 D
CH 28 RTE
I
SK 01
UN 61 D
28 IRTE
SK
UN
Thermal cycler model
Mastercycler
Mastercycler Gradient
Mastercycler ep
Mastercycler M384
‘Real time’ cyclers Mastercycler ep Realplex
Esco
Flexi
Thermal Cyclers
GRI G-Storm
Thermal cyclers
MWG
Thermal cyclers
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Power Block I
Deltacycler I
Deltacycler II
Single Block
Twin Block
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Swift
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Gene
Genius
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GS1
GS4
GSX
GSXs
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Primus 96
Primus 384
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Roche
‘Real time’ cyclers LightCycler 480
Robocycler 96
Robocycler Gradient
‘Real time’ cyclers Mx4000 and Mx3000
TaKaRa
Thermal cyclers
TP240
TP3000
Techne
Thermal cyclers
Touchgene
Cycolgene
Genius
Genius Quad
Genius (TC412)
Flexigene
Touchgene X
Touchgene Gradient (TC512)
‘Real time’ cyclers Quantica
Thermo Hybaid
Thermal cyclers
Transgenomic
Sequencers
PCR Sprint
MBS Satelite (Multiblock) System
Px2 and PxE
PCR Express and Omni-E
Touchdown
Omnigene
WAVE System
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EQUIPMENT
Stratagene
Thermal cyclers
15
■
SOLID PHASE EXTRACTION
Eppendorf
Thermal cyclers
Ericom
Thermal Cyclers
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PalmCycler 96
PalmCycler 384
RotorGene
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W W W. M I C R O P L A T E S . C O M
AUTOM ATION ACCESSORIES
Corbett Research
Thermal cyclers
CUSTOM M ANUFACTURE
Manufacturer
STORAGE/COLLECTION
L I F E S C I E N C E S – P C R P L AT E S P O LY P R O P Y L E N E
STORAGE/COLLECTION
L I F E S C I E N C E S – P C R P L AT E S P O LY P R O P Y L E N E
117.8
117.8
8.89
9.4
7.4
7.4
8.89
77.7
8.89
77.7
20.3
15.24
16
ALL DIMENSIONS IN MM
ALL DIMENSIONS IN MM
286101
286102
118.6
126.2
8.89
SOLID PHASE EXTRACTION
8.89
9.4
9.9
14
11
7.4
8.89
78
86.1
8.89
8.89
5.3
15.24
7.62
ALL DIMENSIONS IN MM
ALL DIMENSIONS IN MM
286103
14
AUTOM ATION ACCESSORIES
286104
126.2
126.2
14
11
11
86.1
86.1
8.89
8.89
CUSTOM M ANUFACTURE
8.89
8.89
EQUIPMENT
15.5
20.3
7.62
20.3
7.62
ALL DIMENSIONS IN MM
ALL DIMENSIONS IN MM
286105
W W W. M I C R O P L A T E S . C O M
286106
127.7
99
STORAGE/COLLECTION
14.5
L I F E S C I E N C E S – P C R P L AT E S P O LY P R O P Y L E N E
128
124.2
14.5
11.2
8.89
11.2
8.89
85.6 63
85.6 82
8.89
8.89
17 15
5.8
15
ALL DIMENSIONS IN MM
ALL DIMENSIONS IN MM
286108 and 286109
SOLID PHASE EXTRACTION
286107
17
125.9
13.4
11
85.8
8.89
8.89
7.62
ALL DIMENSIONS IN MM
ALL DIMENSIONS IN MM
286111
ALL DIMENSIONS IN MM
286112
ALL DIMENSIONS IN MM
286115
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
286110
EQUIPMENT
20.3
STORAGE/COLLECTION
L I F E S C I E N C E S – C H O O S I N G T H E C O R R E C T A S S AY P L AT E
Choosing the correct assay plate
Choosing the correct microplate for your application can mean the
difference between indifferent and great results.
There are three basic methods of obtaining useful optical data from
microplate-based samples. The simplest method is absorbance
measurement. Where greater sensitivity is required, fluorescence
measurements are preferred. Sensitivity can be ten times greater
than simple absorbance measurements. The third method
involves luminescence, a naturally occurring phenomenon
exhibited by certain animal and plant species which can emit light.
This is a biological adaptation of a process which can also be seen as
purely chemically-driven reactions, in which case it is referred to as
chemiluminescence.
Microplate readers are designed to read from either the top or the
bottom of a microplate. Top reading instruments rely on
measuring reflected light above the wells. A good solid bright
white plate is best for these absorbance measurements and a
solid black plate for fluorescence readings. Bottom reading units
illuminate the sample from above and then use detectors placed
below the plate to measure the absorption or
fluorescence/luminescence emission. This necessitates the use of
clear-bottomed plates. The requirement is to transmit the light
wavelengths of interest.
SOLID PHASE EXTRACTION
18
Visible wavelength range (900-350nm) measurements require
only clear plastic bases, whilst readings between 220nm and
350nm will require a UV-transparent material. This can be
Quartz sheet or a modern polymer such as Cyclo-Olefin CoPolymer (COP/COC). Optical glass sheet is used where visible range
detection is combined with confocal optics or whole plate imaging
which requires a very clear uniformly-flat base.
EQUIPMENT
Simple 96-well microplates for ELISA type assays are made from
solid clear polystyrene with no additives. These are adequate for
clinical and diagnostic tests, ELISA assays and any colour endpoint determination with relatively high absorbance.Typically
they are available with flat well bottoms, giving high surface area,
round well bottoms for good mixing or V-wells for high liquid
recovery.
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
Crosstalk can also be an issue in bottom-reading absorbance and
fluorescence measurements. To address this applications
challenge, Porvair Sciences has developed the Krystal 2000
zero-crosstalk plates in which individual clear wells are
moulded into either a white or black matrix. The black or white
base material also projects down below the clear well bottom to
further reduce the possibility of crosstalk.
By carefully selecting the correct plate type for the assay, it is possible
to significantly improve results. By following the simple guidelines
set out here, those tasked with assay development can ensure
that their final assay has the best possible chance of success.
The table summarizes the choices available for Assay Plate
selection and indicates the plate type most likely to give the best
results. Porvair Sciences will be happy to supply samples of their
plates for evaluation free of charge, as different detection systems
and differing assay development may lead to variances in results
between ostensibly similar plate types.
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION
L I F E S C I E N C E S – C H O O S I N G T H E C O R R E C T A S S AY P L AT E
Choosing the correct assay plate
Absorbance assays –
Top Reading Instruments
Bottom Reading Instruments
High signal from majority of wells e.g. test kits, ELISA
Use a simple solid clear plate
Normal visualisation: White plates with clear plastic
bottoms
Low signal from some or all wells e.g. kinetics or
genetic marker assays
Use a solid white plate to boost the signal
Confocal visualisation: White plates with glass bottoms
For very sensitive assays - Zero Crosstalk white plate
with individual clear wells
Fluorescence assays –
Bottom Reading Instruments
Use a solid black plate
Visible wavelength range 350-700nm
High signal from majority of wells e.g. GFP, Reporter
Gene kits,
Use a black clear-bottomed plate
Low signal from some or all wells e.g. poor sensitivity
assays, low specificity
Use a black Zero Crosstalk clear bottom plate to boost
the signal-to-noise ratio
UV Wavelength range 220-350nm
Use black clear-bottomed COP plates
Confocal or whole plate imaging:
Use black glass-bottomed plates
19
SOLID PHASE EXTRACTION
Top Reading Instruments
Luminescence assays –
Bottom Reading Instruments
Bio- and Chemi- Luminescence use the same plates
Good signal
Use solid white plates
Use a white clear-bottomed plate
An introduction to clear bottomed assay microplates
Porvair Krystal series clear bottom plates are now recognised
We supply our Krystal clear plates treated for cell culture
as industry-leading tools for Pharma drug discovery and
with a low pressure gas plasma that produces an even
screening. Our standard clear polystyrene bottom plates
surface treatment right across the plate. It is particularly
have bases just 750µm thick and are moulded from highly
suited to cell lines which do not grow easily on plastics. Our
polished tools to reduce flow lines, artefacts and scratches,
plastic-bottomed plates are ultrasonically welded together –
guaranteeing good results with all types of bottom-reading
a tried and tested method which both guarantees a perfect
instruments. For the more modern demanding confocal
seal every time and eliminates the inconsistencies seen with
readers, Porvair Glass Bottom plates have been shown by
laser membrane welding used by other manufacturers. Only
leading research institutes to be unsurpassed for flatness,
biocompatible adhesives are used for glass bottom plates,
linearity and optical clarity. Our sparkling pure borosilicate
cured by UV radiation to ensure that the finished plates are
glass is just 175µm thick with a flatness tolerance across the
solvent-free, have very low autofluorescence and will allow
plate of +/- 15µm ensuring superb crisp images every time.
good cell growth.
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
Poor signal or high dynamic range across the plate
Use solid Black with White wells
AUTOM ATION ACCESSORIES
EQUIPMENT
Top reading instruments
STORAGE/COLLECTION
L I F E S C I E N C E S – S O L I D B O T T O M A S S AY P L AT E S
For routine adsorption, absorption, ELISA,
mixing and storage applications the standard
range of 96-well assay plates offers the
perfect mix of affordability and high quality.
●
Manufactured from high quality
crystal polystyrene
●
Flat bottom for spectrophotometric
work
●
V-bottom minimizing residual liquid
●
Round (U) bottom for
cell/particulate collection
●
Robot compatible
●
Working volumes from 275µl down
to 10µl
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
20
96-well clear plates
Solid assay plates
CUSTOM M ANUFACTURE
Plate description
Colour
Qty/pack
Cat. no.
96 wells of 350µl with a flat bottom
Clear
100
208004
96 wells of 270µl with a round bottom
Clear
100
209004
96 wells of 220µl with a V bottom
Clear
100
210004
W W W. M I C R O P L A T E S . C O M
96-well black, white &
black with white wells
Porvair Sciences range of solid bottom 96well assay plates is specifically designed for
absorbance, fluorescence, luminescence and
scintillation applications. The design uses the
most popular 96-well format with standard
‘chimney’ wells to overcome optical crosstalk
and contamination.
These plates are designed to give optimum
results from any instrument which reads from
the top of the plate.
Black plates designed for top reading fluorescence
instruments
●
Black plates have low background fluorescence and
minimise light scattering
●
White plates maximise signal for luminescence
readers
●
Highly polished well to give better readings
●
Specially designed plate featuring a white well set in
a black matrix to overcome the problem of a very
high luminescence causing false positives
●
Working volumes of 350µl/well
●
Complies with the standard ANSI/SBS format
●
Alphanumerically labelled wells mean samples can
be easily traced
21
EQUIPMENT
SOLID PHASE EXTRACTION
The acclaimed composite black/white plate
has been shown to be ten times more
sensitive than a standard white plate for
luminesence assays.
●
STORAGE/COLLECTION
L I F E S C I E N C E S – S O L I D B O T T O M A S S AY P L AT E S
Colour
Treatment
Sterile
Lid
Qty/pack
Cat. no.
96 wells
White
–
–
–
50
204503
96 wells
White
–
–
–
100
204003
96 wells
White
Hi protein binding
–
–
100
204006
96 wells
White
Tissue culture
✓
✓
50
204512
96 wells
White
Tissue culture
✓
✓
100
204012
96 wells
Black
–
–
–
50
205503
96 wells
Black
–
–
–
100
205003
96 wells
Black
Hi protein binding
–
–
100
205006
96 wells
Black
Tissue culture
✓
✓
50
205512
96 wells
Black
Tissue culture
✓
✓
100
205012
96 wells
Black with white wells
–
–
–
100
301004
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
Plate description
AUTOM ATION ACCESSORIES
Solid polystyrene assay plates
STORAGE/COLLECTION
L I F E S C I E N C E S – S O L I D B O T T O M A S S AY P L AT E S
384-well assay plates from Porvair Sciences
are available in black, white and clear. These
have been optimised for fluorescence,
luminescence/scintillation and ELISA/turbidity
measurements respectively. There are two
working volumes: 30µl/well or 120µl/well.
All 384-well assay plates are available in
standard, high bind and tissue culture treated
formats.
Round well low volume
Square well plates
The low volume 384-well assay plate is
designed for high throughput screening with
30µl total volume to reduce the amount of
sample and reagent required for the assay.
The 384-well format, with its higher density of wells, allows
more work to be carried out in a standard sized plate, aiding
higher throughput. Well volume is 120µl per well. All of these
plates are manufactured from high quality polystyrene.
●
Specially rounded well to allow
pipette tip access to the bottom of
the well without interference
●
Designed to reduce well-to-well crosstalk
●
Black plates have low background fluorescence and
minimise light scattering
●
Rounded edges to reduce droplet
formation
●
●
Provides a total liquid capacity of
30µl per well
White plates enhance bio- & chemi-luminescence
signals and have low background luminescence and
fluorescence
●
Well working volumes of 100µl
●
Unique moulding technique to
achieve superb flatness to a
tolerance of +/-0.2mm
●
Rounded square at the well bottom to reduce
wicking
●
Low residual volume
●
Standard height plate (14.7mm) for
greater ease of automation
●
Designed to the standard ANSI/SBS format
●
●
Standard ANSI/SBS format
Alphanumerically labelled wells mean samples can
be stored and easily traced
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
22
384-well plates
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION
L I F E S C I E N C E S – S O L I D B O T T O M A S S AY P L AT E S
ROUND WELL
S Q U A R E
W E L L
Plate description
Colour
Treatment
Sterile
Lid
Qty/pack
Cat. no.
384 wells of 120µl
Clear
–
–
–
50
221503
384 wells of 120µl
Clear
–
–
–
100
221003
384 wells of 120µl
Clear
Hi protein bind
–
–
100
221012
384 wells of 120µl
Clear
High bind with lid
–
✓
50
221006
384 wells of 120µl
Clear
Tissue culture
✓
✓
50
221509
384 wells of 120µl
Clear
Tissue culture
✓
✓
100
221009
384 wells of 120µl
White
–
–
–
50
222503
384 wells of 120µl
White
–
–
–
100
222003
384 wells of 120µl
White
Hi protein bind
–
–
100
222012
384 wells of 120µl
White
Hi protein bind with lid
–
✓
50
222006
384 wells of 120µl
White
Tissue culture
✓
✓
50
222509
384 wells of 120µl
White
Tissue culture
✓
✓
100
222009
384 wells of 120µl
Black
–
–
–
50
223503
384 wells of 120µl
Black
–
–
–
100
223003
384 wells of 120µl
Black
Hi protein bind
–
–
100
223012
384 wells of 120µl
Black
Hi protein bind with lid
–
✓
50
223006
384 wells of 120µl
Black
Tissue culture
✓
✓
50
223509
384 wells of 120µl
Black
Tissue culture
✓
✓
100
223009
384 wells of 30µl
Clear
–
–
–
50
221103
384 wells of 30µl
Clear
Tissue culture
✓
✓
50
221109
384 wells of 30µl
White
–
–
–
50
222103
384 wells of 30µl
White
Tissue culture
✓
✓
50
222109
384 wells of 30µl
Black
–
–
–
50
223103
384 wells of 30µl
Black
Tissue culture
✓
✓
50
223109
W W W. M I C R O P L A T E S . C O M
EQUIPMENT
Solid assay plates
AUTOM ATION ACCESSORIES
30µl/well
CUSTOM M ANUFACTURE
120µl/well
SOLID PHASE EXTRACTION
23
STORAGE/COLLECTION
L I F E S C I E N C E S – C L E A R B O T T O M A S S AY P L AT E S
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
24
24-well Krystal™ black &
white
Clear bottom plates allow the cells grown on
the bottom surface to be viewed using an
inverse microscope. In addition, light-emitting
assays can be measured from the bottom.
Porvair Sciences collection of clear bottom 24well Krystal plates are optimised for
fluorescence and luminescence. The special
design of the plate virtually eliminates wellto-well light crosstalk inherent with other
clear-bottomed microplate designs, enabling
unmatched accuracy, higher sensitivity and
better repeatability of photometric readings.
For photometric applications where samples
are typically large, Porvair Sciences range of
unique black or white Krystal 24 plates
provides a perfect solution. Offering 24 high
volume (3.1ml) wells in the same convenient
footprint of a standard 96-well plate, the
Krystal 24 provides a large surface area per
well, enabling efficient cell growth. All tissueculture treated plates are supplied lidded and
sterile, in individual bags.
●
Opaque walls to prevent well-towell crosstalk
●
Clear plate bottom permits direct
microscopic viewing
●
Base plate thickness 0.75mm
●
For use with top or bottom reading
instruments
●
Conforms to the standard ANSI/SBS
format
●
Well volume of 3.1ml
●
Constructed with ultra-pure
polystyrene components polished to
give exceptional photometric
performance and optimised for cell
growth
W W W. M I C R O P L A T E S . C O M
Krystal™ 24-well
Colour
Treatment
Sterile
Lid
Qty/pack
Cat. no.
White
–
–
–
68
303002
White
Hi protein binding
–
–
68
303004
White
Tissue culture
✓
✓
56
303006
Black
–
–
–
68
303008
Black
Hi protein binding
✓
✓
68
303010
Black
Tissue culture
–
–
56
303012
STORAGE/COLLECTION
L I F E S C I E N C E S – C L E A R B O T T O M A S S AY P L AT E S
96-well Krystal™ black
and white
Porvair Sciences clear bottom 96-well Krystal
plates are optimised for fluorescence and
luminescence. Proprietry ultra-sonic welding is
used to assemble the clear bases to the solid
top plate in our clean room. This design
provides flat, optically clear bases to each well
with no leakage, enabling excellent accuracy
and reproducibility of photometric readings.
All tissue-culture treated plates are supplied
lidded and sterile, in individual bags.
●
Clear plate bottom permits direct
microscopic viewing
●
Thickness of bottom 0.75mm
●
For use with top or bottom reading
instruments
●
Standard ANSI/SBS format
●
Well volume of 350µl
●
Constructed from ultra pure grade
polystyrene
Colour
Treatment
Sterile
Lid
Qty/pack
Cat. no.
White
–
–
–
50
214503
White
–
–
–
100
214003
White
Tissue culture
✓
✓
50
214506
White
Tissue culture
✓
✓
100
214006
White
Tissue culture
✓
✓
100 (4 x pack of 25)
214030
Black
–
–
–
50
215503
Black
–
–
–
100
215003
Black
Tissue culture
✓
✓
50
215506
Black
Tissue culture
✓
✓
100
215006
Black
Tissue culture
✓
✓
100 (4 x pack of 25)
215030
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
Krystal™ 96-well
AUTOM ATION ACCESSORIES
EQUIPMENT
Opaque walls to prevent well-towell crosstalk
SOLID PHASE EXTRACTION
25
●
STORAGE/COLLECTION
L I F E S C I E N C E S – C L E A R B O T T O M A S S AY P L AT E S
The Krystal 384-well microplate range
incorporates novel square wells with a large
liquid capacity of 120µl per well, increasing
the achievable sensitivity of most HTS assays.
Using a proprietary manufacturing technique,
the Krystal 384 offers market leading plate
flatness (+/- 0.1mm tolerance) that translates
into a significant increase in measurement
precision and elimination of read errors when
performing cell based assays using fluorescent
or luminescent imaging. All tissue-culture
treated plates are supplied lidded and sterile,
in individual bags.
●
Opaque walls to prevent well-towell crosstalk
●
Clear plate bottom permits direct
microscopic viewing
●
Thickness of bottom 0.40mm
●
For use with top or bottom reading
instruments
●
Maintaining the standard ANSI/SBS
format
●
Well volume of 120µl
●
Constructed from ultra pure grade
polystyrene
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
26
384-well Krystal™ black
and white
Krystal™ 384-well
Colour
Treatment
Sterile
Lid
Qty/pack
Cat. no.
White
–
–
–
100
311001
White
Tissue culture
✓
✓
50
311503
White
Tissue culture
✓
✓
100
311003
White
Tissue culture
✓
✓
100 (4 x pack of 25)
311030
Black
–
–
–
100
312001
Black
Tissue culture
✓
✓
50
312503
Black
Tissue culture
✓
✓
100
312003
Black
Tissue culture
✓
✓
100 (4 x pack of 25)
312030
W W W. P O R V A I R - S C I E N C E S . C O M
96-well Krystal™ 2000
black and white
Raised rims to prevent well-to-well crosstalk
●
Lowered bottom rim to stop lateral light piping
●
Clear well bottom permits direct microscopic
viewing
●
For use with top or bottom reading instruments
●
Designed to the standard ANSI/SBS format
●
Total well volume of 350µl
●
White matrix provides for maximum reflectivity,
allowing high sensitivity luminescence assays
●
Black matrix provides a quenching of background
fluorescence, reducing false positives
●
Constructed from ultra pure grade polystyrene
27
SOLID PHASE EXTRACTION
The unique Krystal 2000 plate range from
Porvair Sciences has been optimised for
luminescence and fluorescence assays.
A patented manufacturing process, using a
‘two-shot’ mould, provides clear, individual
wells in an opaque matrix. The special design
of the plate totally eliminates the well-to-well
optical crosstalk inherent with other clearbottomed microplate designs, giving
unmatched accuracy, sensitivity and
repeatability of photometric readings. Very
high signal-to-noise ratio and low detection
limits can be achieved with this superb plate.
All tissue-culture treated plates are supplied
lidded and sterile, in individual bags.
●
113.30
14.30
11.30
9.00
74.30
STORAGE/COLLECTION
L I F E S C I E N C E S – C L E A R B O T T O M A S S AY P L AT E S
85.60
Cross-section of well
127.60
6.50
Clear cup
Coloured matrix
ALL DIMENSIONS IN MM
Krystal™ 2000 96-well
Colour
Treatment
Sterile
Lid
Qty/pack
Cat. no.
White
–
–
–
50
301510
White
–
–
–
100
301010
White
Hi protein binding
–
–
100
301018
White
Tissue culture
✓
✓
50
301512
White
Tissue culture
✓
✓
100
301012
White
Tissue culture
✓
✓
100 (4 x pack of 25)
301070
Black
–
–
–
50
301502
Black
–
–
–
100
301002
Black
Hi protein binding
–
–
100
301014
Black
Tissue culture
✓
✓
50
301506
Black
Tissue culture
✓
✓
100
301006
Black
Tissue culture
✓
✓
100 (4 x pack of 25)
301060
W W W. M I C R O P L A T E S . C O M
EQUIPMENT
14.70
AUTOM ATION ACCESSORIES
6.00
CUSTOM M ANUFACTURE
11.45
STORAGE/COLLECTION
L I F E S C I E N C E S – G L A S S B O T T O M A S S AY P L AT E S
Manufactured for whole-plate CCD imaging
and laser detection applications, Krystal glass
bottom plates consist of a polystyrene upper
part and a clear borosilicate glass sheet fixed
to the base with a proprietary adhesive. This
process results in consistent flatness of the
base and gives improved light transmission
whilst maintaining a flat optical plane for
growing cells. The nominal cut-off
wavelength of 335nm allows most
fluorescence assays to be excited or read
through the glass bottom. All plates are
supplied lidded.
●
175µm glass thickness
●
+/- 15µm flatness across base
●
335nm UV optical cut-off
●
Industry-standard well layout
●
Very low autofluorescence
●
High degree of planar flatness
●
Biocompatible adhesive
●
Resistant to alcohol, DMSO and PBS
●
Robot friendly
●
Manufactured to ANSI/SBS specification
Krystal™ 24-well glass
bottom plates
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
28
Krystal™ glass bottom
plates
CUSTOM M ANUFACTURE
Glass bottom assay plates
Number of wells
Description
Sterile
Lid
Pack size
Cat. no.
24 wells
Black with lid, single pack
–
✓
10
324041
24 wells
White with lid, single pack
–
✓
10
324051
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION
L I F E S C I E N C E S – G L A S S B O T T O M A S S AY P L AT E S
Krystal™ 96-well glass
bottom plates
Glass bottom assay plates
Number of wells
Description
Sterile
Lid
Pack size
Cat. no.
96 wells
Black with lid, single pack
–
✓
10
324001
96 wells
White with lid, single pack
–
✓
10
324011
Description
Sterile
Lid
Pack size
Cat. no.
384 wells
Black with lid, single pack
–
✓
10
324021
384 wells
White with lid, single pack
–
✓
10
324031
SOLID PHASE EXTRACTION
29
AUTOM ATION ACCESSORIES
EQUIPMENT
Krystal™ 384-well glass
bottom plates
Number of wells
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
Glass bottom assay plates
STORAGE/COLLECTION
L I F E S C I E N C E S – U V C L E A R B O T T O M A S S AY P L AT E S
Recently, scientists have begun using assay
chemistries which require excitation or
detection wavelengths in the far UV region,
below 350nm. To enable our customers to
develop assays in this area, Porvair Sciences
has introduced a very high specification range
of COP-bottomed, UV-transparent
microplates. Precision engineered and
assembled, these plates are available in 96and 384-well black matrix only for the most
sensitive UV range fluorescence assays using
whole-plate imaging or confocal microscopy.
●
220nm far UV cut-off
●
96- and 384-well black versions
●
Industry-standard well layout
●
Very low autofluorescence
●
High degree of planar flatness
●
High chemical resistance to most
solvents
●
Robot friendly
●
Cyclo Olefin Polymer construction
with clear base
●
Precision engineered to meet
ANSI/SBS specification
EQUIPMENT
SOLID PHASE EXTRACTION
30
Krystal UV clear
bottomed microplates
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
Overall plate height = 13.06mm
Krystal UV COP-bottomed microplates
Pack
size
Cat. no.
96-well Krystal
COP-bottomed plate
32
327002
384-well Krystal
COP-bottomed plate
32
327001
Description
Detailed schematics available on request
W W W. M I C R O P L A T E S . C O M
Overall plate height = 14.35mm
STORAGE/COLLECTION
L I F E S C I E N C E S – F I LT R AT I O N P L AT E S
Filtration plates
Filtration plates are used in their simplest form to
remove particulate matter from liquid. Either the
particulate matter or the filtrate is needed for further
study.
Porvair Sciences has a range of filter plates to suit
most filtration applications. The company has
optimised filter plates for applications including cell
harvesting, DNA separations, binding studies, Plasmid
isolation, general filtration and sample clean up.
Porvair has a full range of 48-, 96- and 384-well
microplates with a choice of glass fibre, nylon, PVDF
or polyethylene filtration materials and well volumes
ranging from 350µl to 5ml.
●
Long and short drip directors
●
Each well has an individual drainage spout
ensuring 100% sample transfer
●
Manufactured from ultra pure grade polymer
●
Standard ANSI/SBS footprint
●
Robot friendly
●
Fit standard vacuum manifold
SOLID PHASE EXTRACTION
Polystyrene and polypropylene filter plates
350µl/well
800µl/well
5ml/well
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
●
31
STORAGE/COLLECTION
L I F E S C I E N C E S – F I LT R AT I O N P L AT E S
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
32
Filtration plates
No & vol. of wells Plate, filter material and pore size
Qty/pack
Cat. no.
48 wells of 7.5ml
Polypropylene, polyethylene, mean pore size 25 microns, long drip
15
360053
48 wells of 5ml
Polypropylene, polyethylene filter, mean pore size 10 to 20 microns
25
360008
96 wells of 300µl
Polypropylene, UHMW polyethylene, mean pore size 25 microns, short drip
50
360047
96 wells of 300µl
Polypropylene, UHMW polyethylene, mean pore size 25 microns, long drip
50
360041
96 wells of 300µl
Polypropylene, glass fibre, mean pore size 0.7 microns, short drip
50
360052
96 wells of 300µl
Polypropylene, glass fibre, mean pore size 0.7 microns, long drip
50
360046
96 wells of 300µl
Polypropylene, glass fibre, mean pore size 1.0 microns, short drip
50
360051
96 wells of 300µl
Polypropylene, glass fibre, mean pore size 1.0 microns, long drip
50
360045
96 wells of 300µl
Polypropylene, glass fibre, mean pore size 1.2 microns, short drip
50
360048
96 wells of 300µl
Polypropylene, glass fibre, mean pore size 1.2 microns, long drip
50
360042
96 wells of 300µl
Polypropylene, glass fibre, mean pore size 3.0 microns, long drip
50
360066
96 wells of 300µl
Polypropylene, hydrophilic PVDF, mean pore size 0.45 microns, short drip
50
360049
96 wells of 300µl
Polypropylene, hydrophilic PVDF, mean pore size 0.45 microns, long drip
50
360043
96 wells of 300µl
Polypropylene, polypropylene, mean pore size 0.45 microns, short drip
50
360050
96 wells of 300µl
Polypropylene, polypropylene, mean pore size 0.45 microns, long drip
50
360044
96 wells of 300µl
Polypropylene, PVDF, mean pore size 1.0 microns with a polyprop back, short drip
50
360070
96 wells of 300µl
Polypropylene, black, hydrophilic PVDF, mean pore size 0.2 microns, short drip
50
360060
96 wells of 300µl
Polypropylene, black, polyproylene, mean pore size 0.45 microns, short drip
50
360064
96 wells of 350µl
Polystyrene, glass fibre, mean pore size 1.9 microns
50
230001
96 wells of 350µl
Polystyrene, glass fibre, mean pore size 1.3 microns
50
230002
96 wells of 350µl
Polystyrene, glass fibre, mean pore size 1.7 microns
50
230003
96 wells of 350µl
Polystyrene, glass fibre, mean pore size 6.0 microns
50
230004
96 wells of 400µl
Polypropylene, UHMW polyethylene, mean pore size 25 microns, short drip
25
360035
96 wells of 400µl
Polypropylene, UHMW polyethylene, mean pore size 25 microns, long drip
25
360026
96 wells of 400µl
Polypropylene, glass fibre, mean pore size 0.7 microns, short drip
25
360040
96 wells of 400µl
Polypropylene, glass fibre, mean pore size 0.7 microns, long drip
25
360017
96 wells of 400µl
Polypropylene, glass fibre, mean pore size 1.0 microns, short drip
25
360039
96 wells of 400µl
Polypropylene, glass fibre, mean pore size 1.0 microns, long drip
25
360028
96 wells of 400µl
Polypropylene, glass fibre, mean pore size 1.2 microns, short drip
25
360036
96 wells of 400µl
Polypropylene, glass fibre, mean pore size 1.2 microns, long drip
25
360016
96 wells of 400µl
Polypropylene, hydrophilic PVDF, mean pore size 0.45 microns, short drip
25
360037
96 wells of 400µl
Polypropylene, hydrophilic PVDF, mean pore size 0.45 microns, long drip
25
360027
96 wells of 400µl
Polypropylene, polypropylene, mean pore size 0.45 microns, short drip
25
360038
96 wells of 400µl
Polypropylene, polypropylene, mean pore size 0.45 microns, long drip
25
360020
96 wells of 400µl
Polypropylene, PES, pore size 10K, short drip
25
360061
96 wells of 400µl
Polypropylene, PES, pore size 30K, short drip
25
360062
W W W. M I C R O P L A T E S . C O M
Cat. no.
96 wells of 800µl
Polypropylene, UHMW polyethylene, mean pore size 25 microns,
short drip
25
360029
96 wells of 800µl
Polypropylene, glass fibre, mean pore size 0.7 microns, short drip
25
360034
96 wells of 800µl
Polypropylene, glass fibre, mean pore size 0.7 microns, long drip
25
360025
96 wells of 800µl
Polypropylene, glass fibre, mean pore size 1.0 microns, short drip
25
360033
96 wells of 800µl
Polypropylene, glass fibre, mean pore size 1.0 microns, long drip
25
360024
96 wells of 800µl
Polypropylene, glass fibre, mean pore size 1.2 microns, short drip
25
360030
96 wells of 800µl
Polypropylene, glass fibre, mean pore size 1.2 microns, long drip
25
360022
96 wells of 800µl
Polypropylene, glass fibre, mean pore size 3.0 microns, long drip
25
360075
96 wells of 800µl
Polypropylene, glass fibre, mean pore size 13.0 microns, long drip
25
360076
96 wells of 800µl
Polypropylene, glass fibre, mean pore size 0.7 microns and a
polyethylene frit, long drip
25
360065
96 wells of 800µl
Polypropylene, hydrophilic PVDF, mean pore size 0.45 microns, short drip 25
360031
96 wells of 800µl
Polypropylene, hydrophilic PVDF, mean pore size 0.45 microns, long drip
25
360023
96 wells of 800µl
Polypropylene, polypropylene, mean pore size 0.45 microns, short drip
25
360032
96 wells of 800µl
Polypropylene, polypropylene, mean pore size 0.45 microns, long drip
25
360019
96 wells of 800µl
Polypropylene, polyethylene, mean pore size 10 to 20 microns, long drip
25
360011
96 wells of 800µl
Polypropylene, Hydrophilic CA, mean pore size 0.2 microns, long drip
25
360055
96 wells of 2ml
Polypropylene, UHMW PE, mean pore size 25 microns, long drip
25
360056
96 wells of 2ml
Polypropylene, glass fibre, mean pore size 0.7 microns, long drip
25
360057
96 wells of 2ml
Polypropylene, polycarbonate, mean pore size 2.0 microns, long drip
25
360071
96 wells of 2ml
Polypropylene, glass fibre, mean pore size 3.0 microns and
polypropylene, mean pore size of 10 microns, long drip
25
360063
96 wells of 2ml
Polypropylene, polypropylene, mean pore size 0.45 microns, long drip
25
360058
96 wells of 2ml
Polypropylene, polyethylene mean pore size 25 microns, long drip
25
360021
96 wells of 2ml
Polypropylene, Hydrophilic PES, mean pore size 0.45 microns, long drip
25
360059
96 wells of 2ml
Polyproylene, glass fibre, 5.0 microns, long drip
25
360114
96 wells of 2ml
Polyproylene, Affymetrix, SNP 5.0 assay dedicated plate
10
360090
384 wells of 140µl
Polypropylene, glass fibre 0.7 microns, short drip
10
360068
384 wells of 140µl
Polypropylene, glass fibre 0.7 microns, long drip
10
360073
384 wells of 140µl
Polypropylene, glass fibre 1.2 microns, short drip
10
360067
384 wells of 140µl
Polypropylene, glass fibre 3.0 microns, short drip
10
360069
96-well filter plate, 300µl
Hydrophilic PVDF 1.2µm, short drip
50
360105
384-well plate, 140µl
No filter, long drip
10
360107
384-well filter plate, 140µl
GF 5.0µm, long drip
10
360108
96-well filter plate, 250µl
UHMW PE 25µm, short drip
25
360109
96-well filter plate, 300µl
GF 1.0µm, medium drip
50
360110
48-well plate, 7ml
No filter, long drip directors
30
360111
W W W. M I C R O P L A T E S . C O M
33
SOLID PHASE EXTRACTION
Qty/pack
EQUIPMENT
Plate, filter material and pore size
AUTOM ATION ACCESSORIES
No & vol. of wells
CUSTOM M ANUFACTURE
Filtration plates
STORAGE/COLLECTION
L I F E S C I E N C E S – F I LT R AT I O N P L AT E S
STORAGE/COLLECTION
L I F E S C I E N C E S – F I LT R AT I O N P L AT E S
Filter bottom microplate application guide
Solvent resistant
Application
Filter
Drip detector
Vol (µl/well)
Cat. no.
Low Biomolecule Binding
YAC (Yeast Cloning Assays)
DNA extraction from agarose gel
Dye Terminator Clean Up
Sequencing Reaction Clean Up
DNA/RNA Purification
Ni-Nta
Affinity Bead
Streptavidin/biotin bead
Chromatography beads/resins
Purified cloned receptors
Cell Based Receptor Binding
Signal transduction
Phosphodiesterase
Protein or nucleic acid desalt
Cell membrane
Neonatal Screening
Vesicle Assay
Dye Terminator Removal
Dye Terminator Clean Up
Sequencing Reaction Clean Up
Hydrophilic
PVDF 0.45µm
Short
300
400
800
360043
360037
360031
Long
300
400
800
360049
360027
360023
Short
300
400
800
300
400
800
2000
2000
5000
300
400
800
360047
360035
360029
360041
360026
360011
360056
360021
360008
360050
360038
360032
Long
300
400
800
2000
360044
360020
360019
360058
140
400
800
2000
300
400
800
360073
360017
360025
360057
360051
360039
360033
Long
300
400
800
360045
360028
360024
Short
140
300
400
800
140
300
400
800
360067
360048
360036
360030
360072
360042
360016
360022
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
34
Low Biomolecule Binding
UHMW PE
25µm
Long
Low Biomolecule Binding
Solid Phase Extraction (SPE)
P E Frit 25µm
Long
Low Biomolecule Binding
YAC (Yeast Cloning Assays)
Bacterial DNA prep for PCR
Alumina
Cell fragments/membranes
Whole Cells
Protease assays
Phosphodiesterase
Antibody neutralization
Mammalian Cells Capture
Calcium uptake
Dual assay: Ca + receptor
Microsomes
Solid Phase Radioimmunoassays
DNA Binding
Lysate Clarification
Polypropylene
0.45µm
Short
Glass fibre
0.7µm
Long
Cell homogenates. crude
DNA Binding
DNA Isolation
DNA/RNA Purification
PCR Clean up
Plasmid Minipreps
Recovering DNA from gels
Reverse Transcriptase
Sample Clean up
M13 phage preps
Thymidine Uptake
Cell homogenates, crude
Glass fibre
1.0µm
Short
High Biomolecule Binding
High Biomolecule Binding
High Biomolecule Binding
Cell fragments/membranes
Cytotoxic/Cell Proliferation
Signal transduction
Mammalian Cells Capture
W W W. M I C R O P L A T E S . C O M
Glass fibre
1.2µm
Long
Bacti-growth plates
Bacti-growth plates are specially packaged
plates to allow the growth of bacteria, yeast,
mammalian or insect cell lines.
Using only the highest-grade raw materials,
our plates are tested to ensure no moulding
or polymer contaminants leach out into the
samples. This guarantees reproducibility and
maintains customer confidence. Each Bactigrowth plate consists of a deep well plate and
a tight fitting lid, packed sterile in bags.
●
Plate well configuration available from 24- through
to 384-well
●
Well volumes from 300µl through to 10ml per well
●
Round and square well available
●
Sealed in sterile bags of 5 plates
●
Robot friendly
●
Manufactured to the highest specifications
●
Conform to the ANSI/SBS format
STORAGE/COLLECTION
L I F E S C I E N C E S – G R OW T H P L AT E S
Bacti-growth plates
Description
Sterile
Lid
Qty/Pack
Cat. no.
24 x 10ml square wells, polypropylene with lid, sterile
✓
✓
25
219115
96 x 1ml round wells, polypropylene with lid, sterile
✓
✓
25
219101
96 x 2ml square wells, polypropylene with lid, sterile
✓
✓
25
219102
384 x 300µl square wells, polypropylene with lid, sterile
✓
✓
20
219103
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
35
STORAGE/COLLECTION
L I F E S C I E N C E S – G R OW T H P L AT E S
219115
219101
219102
219103
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
36
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION
SOLID PHASE EXTRACTION
MicroLute™
LIFE SCIENCES
Solid phase extraction (SPE) is a method of
sample preparation that concentrates and
purifies analytes from solution by sorption,
followed by elution of the analyte with
a solvent appropriate for instrumental
analysis, such as LC-MS. Porvair Sciences
offers a complete range of products to
implement SPE.
MicroLute™ is the original 96-well plate
format for SPE. This device consists of a
polypropylene 96-well plate loaded with a
choice of sorbent and sorbent volumes. The
frits have a mean pore size of 20 microns and
are made from microporous high-density
polyethylene.
Packed bed volumes from 10mg to
100mg per well
●
Frit pore size 20µm
●
No channelling due to proprietary
sorbent loading technique
●
Working volume of 2ml per well
●
Up to four times quicker than
cartridge systems
●
Long drain directors locate
accurately with collection plate to
avoid cross contamination
●
Virgin polypropylene, tested and
chosen for having no extractables
●
Plate designed to be automated,
meets SBS/ANSI specification
37
EQUIPMENT
●
AUTOM ATION ACCESSORIES
Choice of popular sorbents
Porvair's flexible manufacturing
approach enables us to consider
manufacture of small runs of
MicroLute™ devices packed with
specialty resins or sorbents of your
choice. Typically, minimum runs of just
50 plates can be produced
economically. Please contact Porvair
Sciences for more details of this service.
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
●
LIFE SCIENCES
STORAGE/COLLECTION
SOLID PHASE EXTRACTION
AUTOM ATION ACCESSORIES
EQUIPMENT
38
MicroLute™ packed with Varian sorbent
Description
C18
C8
C2
C1
PH
CH
CN-E
C18-OH
CN-N
20H
Si
CBA
PRS
SCX
NH2
PSA
DEA
SAX
PBA
ENV
LMS
PPL
C18HF
Certify
Certify HF
Certify II
Certify II HF
10mg
242001
242002
242003
242004
242005
242006
242007
242008
242009
242010
242011
242012
242013
242014
242015
242016
242017
242018
242019
242020
242021
242022
242023
242024
242025
242026
242027
15mg
244001
244002
244003
244004
244005
244006
244007
244008
244009
244010
244011
244012
244013
244014
244015
244016
244017
244018
244019
244020
244021
244022
244023
244024
244025
244026
244027
25mg
246001
246002
246003
246004
246005
246006
246007
246008
246009
246010
246011
246012
246013
246014
246015
246016
246017
246018
246019
246020
246021
246022
246023
246024
246025
246026
246027
100mg
250001
250002
250003
250004
250005
250006
250007
250008
250009
250010
250011
250012
250013
250014
250015
250016
250017
250018
250019
–
–
–
–
250024
–
250026
–
Combinatorial
MicroLute™
Combinatorial MicroLute™ is based on the
tried and tested MicroLute™ format. This is a
96-well polypropylene plate with a bottom
polyethylene frit already in place. It is
designed to allow packing with any material
required for chemical synthesis. Two bottom
frit sizes are available, 10µm and 20µm,
however, we recommend the use of 20µm
top frits to ensure a good flow rate in both
cases.
●
Accepted industry standard
●
Up to 2ml per well of sample
●
Low frit liquid retention
●
Virgin polypropylene
●
Polypropylene is tested and chosen for having no
extractables
●
Manufactured to SBS/ANSI standard to allow for
automation
Combinatorial MicroLute™
Description
MicroLute™ plate, unpacked with bottom frits, Mean pore size 20µm
Mean pore size 20µm, bag of 1000 frits, polyethylene
CUSTOM M ANUFACTURE
50mg
248001
228002
248003
248004
248005
248006
248007
248008
248009
248010
248011
248012
248013
248014
248015
248016
248017
248018
248019
248020
248021
248022
248023
248024
248025
248026
248027
MicroLute™ plate, unpacked with bottom frits, Mean pore size 10µm
Mean pore size 10µm, bag of 1000 frits, polyethylene
Empty 96-well MicroLute shell with no top or bottom frits
W W W. M I C R O P L A T E S . C O M
Qty/pack
Cat. no.
1
240002
1,000
239007
1
240054
1,000
239010
40
240011
STORAGE/COLLECTION
SOLID PHASE EXTRACTION
MaxiLute™
The development of a 48-well solid-phase
extraction (SPE) system allows the step
between SPE cartridges and SPE 96-well
plates to be managed.
LIFE SCIENCES
With the larger packed bed volume and
increased sample volume, the design of the
MaxiLute™ offers advantages for drug
development, food and environmental
laboratories currently using SPE cartridges.
Manufactured from virgin polypropylene and
loaded with a choice of sorbents and
volumes.
Up to 4 times faster than cartridge
methods
●
Constructed from a single piece of
high quality polymer which will not
bend or distort
●
A range of packed bed volumes
ranging from 250mg, through to
1500mg
●
Capability to process up to 200ml of
sample in one plate
●
Frit pore size of 20µm
●
Each well has an individual drain
spout ensuring 100% sample
transfer and zero crosscontamination
●
Manufactured within SBS/ ANSI
standard to allow easy automation
39
EQUIPMENT
●
AUTOM ATION ACCESSORIES
Unique design eliminates mess
associated with traditional manual
SPE cartridge methods
MaxiLute™
Sorbent
volume
250mg
500mg
1000mg
1500mg
SCX
322001
321001
318001
320001
SAX
322002
321002
318002
320002
Silica
322003
321003
318003
320003
C18
322004
321004
318004
320004
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
●
LIFE SCIENCES
STORAGE/COLLECTION
SOLID PHASE EXTRACTION
P3 Protein Precipitation Plates
Biological samples commonly contain proteins
that interfere with downstream applications.
The P3 plate uses the CRASH method, in which
the protein is denatured with acetronitrile and
the flocculant filtered out, allowing 96 samples
to be handled at one time.
The Protein Precipitation Plate, P3, is based on
the industry standard MicroLute™ format, but
without the chromatographic sorbent. Protein
‘crashes’ out of solution and precipitates
directly in each well when acetonitrile is added,
thus solving all common problems associated
with the CRASH technique of protein clean-up.
The novel dual frit, hydrophobically treated
matrix means that there is no ‘wetting out’ and
leakage of the sample through the plate before
the application of vacuum. P3 is now
commonly used as the protein precipitation
plate of choice in many major pharmaceutical
companies.
AUTOM ATION ACCESSORIES
EQUIPMENT
40
Optional drain cap and top cap mats are
available for those wishing to use vortex
sample mixing (see page 41).
●
Dual frit design
●
Pre-filter frit at 100µm traps large
flocculant particles
●
Secondary frit traps fine protein
particles at <10µm
●
Frits are Hydrophobic/Oleophobic.
This retains sample/acetonitrile in
the well to allow precipitation of
the proteins until vacuum is applied.
●
Pore size optimised to allow ideal
flow rate
●
Inert filter material to ensure no
adsorption of sample components
●
Frit structure prevents break through
of protein particles
●
Industry standard MicroLute™
format enables easy automation
●
Specially selected polypropylene for
low extractables
Protein Precipitation Plate
CUSTOM M ANUFACTURE
Description
*The Protein MicroLute High Efficiency plate is exactly the same
as the P3 but with the frits untreated. This plate is used when
sample and acetronitrile are pre-mixed before being pipetted
into the plate.
W W W. M I C R O P L A T E S . C O M
P3
P3
Qty/pack
Cat. no.
Protein Precipitation Plate (single pack)
1
240100
Protein Precipitation Plate (bulk pack)
25
240200
1
240010
Protein MicroLute™ High Efficiency*
SPE starter pack
Development MicroLute™
This kit has been designed to form the
starting point for SPE analysis. This gives the
researcher the necessary hardware to process
samples using an SPE plate and to collect
under vacuum the analyte for further analysis.
This is an SPE Development MicroLute™, which is designed
for research and development laboratories, where it may not
be known which sorbent best suits the process.
●
Uses an industry standard SPE plate, MicroLute™
●
Sorbents from Varian
●
1 x acrylic manifold
●
Packed bed volumes ranging from 10mg to 100mg
●
1 x 1ml spacer insert
●
Allows up to 2ml of sample to be tested
●
3 x 2ml 96-well microplates
●
Can be packed either in 8-row or 12-column format
●
3 x disposable reservoir trays
●
Manufactured within SBS/ANSI standard to allow
for automation
Starter pack
271023
MicroLute™ – Development
10mg
15mg
25mg
50mg
100mg
Eight sorbents
271014
271016
271018
271020
271022
Twelve sorbents
271024
271026
271028
271030
271032
MicroLute™ accessories
●
Disposable reservoir tray. Used for the collection of
waste products in the vacuum manifold
●
Sealing cap, square well, fits top of MicroLute™.
Used for retaining the sample in the well during
transit or stopping the well from becoming
contaminated
●
Drain cap mat that seals the bottom of the plate,
preventing liquid leakage
MicroLute™ – accessories
Description
41
EQUIPMENT
MicroLute™ starter pack
LIFE SCIENCES
2 x 50mg MicroLute™ – containing:
50mg of Varian C18, C8, C2, SAX,
SCX, Certify, Certify II and CBA,
1 x 50mg Varian C18
Qty/pack
Cat. no.
Disposable reservoir tray
25
219010
Sealing cap, square well, fits to top of plate
50
219004
Drain cap mat, seals base of plate
25
219005
W W W. M I C R O P L A T E S . C O M
AUTOM ATION ACCESSORIES
●
The system allows the researcher to specify which sorbents go
into specific wells, with what designated packed bed volume.
This allows the researcher to have full control of the SPE
plate.
CUSTOM M ANUFACTURE
The kit consists of:
STORAGE/COLLECTION
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
SOLID PHASE EXTRACTION
MicroLute™ BioVyo
Co-sinter products
BioVyon™ Co-sinter products are made using
a solid-state media created from an ultra-pure
and highly modified polymeric material with
the lowest levels of extractables and
leachables. Our proprietary BioVyon™
technology allows high-purity silica resins to
be supported in a matrix which provides a
high surface area whilst reducing channelling
through the column. As the porous material is
co-sintered under high pressure and
temperature with the BioVyon™ polymer
matrix, the resultant frit or column is
impervious to further compression and easily
resists channelling of liquids, even at high
flow rates.
C8 and C18
products
Micro structure of BioVyon substrate
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
42
Porvair Sciences have introduced MicroLute™
BioVyon™ Co-sintered C8 and C18 silica SPE
96-well plates for low volume assays. Offered
in 96-well MicroLute™ plates as a 10mg per
well loading of C8 or C18 suitable for low
sample volume bioassay preps and clean ups.
A MicroLute solid phase extraction plate in
use with the new Porvair Sciences robotic
compatible vacuum manifold.
Micro structure of BioVyon substrate
MicroLute™ BioVyon™ Co-sinter products
Solid Phase Extraction
Description
Qty/pack
Cat. no.
C8 96-well plate 10mg per well
1
240030
C18 96-well plate 10mg per well
1
240031
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION
E Q U I P M E N T – VA C U U M M A N I F O L D S
Vacuum manifolds
Vacuum manifolds are used to draw liquid
through a filter or SPE plate into either a waste
tray or a collection plate. The application of
vacuum increases the speed at which samples
can be collected.
LIFE SCIENCES
MicroLute™ manifold
●
Also designed so that SPE plates can
be used, especially the Porvair
Sciences MicroLute™ plate
●
Fitted with valve controller to ensure
accurate adjustments of vacuum to
the manifold
●
On/off valve for speed of use
●
Square well collection plate volumes
of 350µl, 1ml and 2ml may be used.
Any plate up to 44mm in height can
be used
●
Fitted with a custom O-ring in upper
surface allowing airtight interface
between plates during operation
●
Removable top plate to install
reservoir tray or collection plate
●
Chamber has a medium resistance
to alcohols and weak acids
43
MicroLute™ manifold
Description
Qty/pack
Cat. no.
Standard MicroLute™ manifold to hold deep 96-well collection plate
1
228008
Replacement gasket, profile (to fit between top plate and vacuum chamber), for 228008/228020
1
228007
Replacement gasket, flat (to fit top plate below filtration plate), for 228008/228020
1
228009
Optional spacer insert, polypropylene, to allow use of 1ml round well polypropylene microplates (219002)
in acrylic deep well manifolds (228008/228020)
1
228010
Optional spacer insert, HDPE, 350µl, to allow use of 350µl microplates in acrylic manifolds (228008/228020)
1
228012
Disposable reservoir tray, PVC
25
219010
Single plate manifold, high impact polystyrene, for filter plates (no waste collection) (not shown)
1
228001
W W W. M I C R O P L A T E S . C O M
AUTOM ATION ACCESSORIES
Designed to take most filter plates
manufactured to the SBS/ANSI
specification with long drip directors
CUSTOM M ANUFACTURE
●
SOLID PHASE EXTRACTION
The MicroLute™ vacuum manifold from Porvair
Sciences is precision machined from crystal clear
acrylic (top plate) and acetal polymer (plenum
chamber). The acrylic top plate allows visual
access to the plenum chamber for checking
progress of the separation process.
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
E Q U I P M E N T – VA C U U M M A N I F O L D S
Universal robotic
manifold
Based on our successful MicroLute™ Acrylic
Manifold, the new Universal Robotic
Manifold is designed to be easily assembled
and disassembled by robotic manipulators,
thus enabling productive automation of SPE
or DNA clean-up procedures. Able to
accommodate collection plates from 14mm 44mm in height and adaptable for working
with short, medium and long skirts and drip
directors, the Universal Robotic Manifold can
operate with many different brands of filter
plate and collection trays. Supplied with an
integral vacuum gauge, the Manifold provides
complete control of vacuum pressure ensuring
plates are processed with high reproducibility.
The Manifold is fully compatible with most
commercial robotic liquid handling systems.
The Universal Robotic Manifold is suitable for
a range of manufacturer’s plates without an
adaptor. For certain plate types a Shallow
Adaptor or a Deep Adaptor, is required.
●
Compatible with any filter plate type
●
Robotic friendly designs aids
automation
●
Chemically resistant acetal/acrylic
construction
●
Adaptable for different collection
plates
●
Easy visual inspection of process
●
Built in vacuum gauge for
reproducibility
AUTOM ATION ACCESSORIES
44
Manufacturer
Plate type
Qiagen™
Waters™
Standard plate
Waters™
µElution plate
Biotage™
Varian™
Phenomenex™
Axygen™
Seahorse™
Porvair™
Base
Adapt. 1
■
■
■
■
■
■
■
■
■
■
Adapt. 2
■
■
Universal robotic manifold
CUSTOM M ANUFACTURE
Description
Qty/pack
Cat. no.
Universal robotic compatible manifold to hold deep 96-well collection plate
1
228020
Adaptor 1 for medium skirt/medium drip director plates
1
228021
Adaptor 2 for short skirt/long drip director plates
1
228022
Gaskets and spacers are identical to those for our standard MicroLute™ acrylic manifold shown on page 43.
W W W. M I C R O P L A T E S . C O M
Porvair evaporators are designed to remove
the traditional laboratory ‘bottleneck’ of
solvent evaporation from microplates prior to
analysis or reconstitution in storage buffer.
These evaporators give significant throughput
advantages to laboratories looking to optimise
microplate sample preparation productivity.
Faster than centrifugal evaporation,
significant increases in sample throughput are
achieved through advanced evaporator head
technology and an innovative manifold
design, which directly injects heated nitrogen
into each individual well of the microplate
simultaneously. The evaporators have been
designed to be simple to install, operate and
maintain. Installation requires only connection
to a gas supply or cylinder and mains
electricity. Safety of operation is ensured as
the CE marked compact units fit into all fume
cupboards. Not suitable for high boiling
solvents such as DMSO and water.
The MiniVap is purpose designed for low usage research and
development departments where low numbers of individual
plates or vials need drying. The MiniVap is simple to operate
and maintain. Installation requires only connection to a gas
supply and standard mains socket. With manual control of the
needle depth, gas temperature and flow rate, it allows fine
control and quicker drying times than other standard methods.
Quicker dry down times than standard methods such
as vacuum oven
●
Tests have shown that the MiniVap can evaporate
500µl of methanol in less than 6 minutes
●
Designed for any SBS/ANSI 96 well plate
●
24 vial head now available
●
Simple to install and operate
●
Easy adjustments of temperature, gas flow rates and
needle depth into the wells
●
Compact footprint – fits all standard fume cupboards
MiniVap™ blowdown sample concentrator
Description
Qty/pack
Cat. no.
MiniVap™ (230 Volts), with 96 spiral needles
1
229201
MiniVap™ (230 Volts), with 96 straight needles
1
229200
MiniVap™ (230 Volts), with 24 straight needles
1
229205
MiniVap™ (110 Volts), with 96 spiral needles
1
229203
MiniVap™ (110 Volts), with 96 straight needles
1
229202
MiniVap™ (110 Volts), with 24 straight needles
1
229204
MiniVap
AUTOM ATION ACCESSORIES
45
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
Both MiniVap and UltraVap may be operated
with a supply of clean, dry compressed air in
place of nitrogen, if the chemistry allows. An
in-line gas filter must be used in this case.
●
LIFE SCIENCES
MiniVap™
SOLID PHASE EXTRACTION
Microplate evaporators
STORAGE/COLLECTION
E Q U I P M E N T – M I C R O P L AT E E VA P O R AT O R S
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
E Q U I P M E N T – M I C R O P L AT E E VA P O R AT O R S
Ultravap™
Whereas the MiniVap™ is a fully manual
system, the Ultravap™ is a digitally controlled
programmable evaporator. It can be used as a
stand alone unit or driven by a computer,
often connected to a robotic liquid handler.
After empirical data for dry down has been
produced, this can be entered into the
memory of the Ultravap for future recall and
use. Each programme has three stages, with
control of gas temperature, pressure and plate
height at each stage. Nine different methods
can be permanently stored in memory. The
Ultravap will then evaporate plate contents
quickly, by keeping the heated gas needles
close to the liquid surface at all times in the
concentration process, without further
manual intervention.
●
●
●
●
●
●
●
●
●
Designed for heat sensitive and thermally labile
samples
Nine different drying programmes can be retained in
the memory
Ability to upload and download methods
Robotic plate stage automatically moves plates up
and down
RS232 allows remote control via PC or LH robot
Removes most stubborn solvents in just minutes
Can evaporate 500µl methanol in 5 minutes
Compact footprint allows the Ultravap to fit into all
standard fume cupboards
Choice of straight, curved & 24, 96 or 384 needles
Compatible with Tecan™, Hamilton™ and
Beckman™ robotic liquid handlers
Ultravap™ high speed sample concentrator
Description
Qty/pack
Cat. no.
Ultravap™ 24 Straight needles, 230V, for 24-well plates and vial carriers
1
229138
Ultravap™ 96 with spiral needles, 230V, for 96-well plates
1
229070
Ultravap™ 96 with straight needles, 230V, for 96-well plates
1
229135
Ultravap™ 384 with straight needles, 230V, for 384-well plates
1
229071
Ultravap™ 24 Straight needles, 110V, for 24-well plates and vial carriers
1
229139
Ultravap™ 96 with spiral needles, 110V, for 96-well plates
1
229080
Ultravap™ 96 with straight needles, 110V, for 96-well plates
1
229136
Ultravap™ 384 with straight needles, 110V, for 384-well plates
1
229081
UltraVap
AUTOM ATION ACCESSORIES
46
CUSTOM M ANUFACTURE
●
W W W. M I C R O P L A T E S . C O M
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
E Q U I P M E N T – M I C R O P L AT E E VA P O R AT O R S
MiniVap 229205
Description
Qty/pack
Cat. no.
Replacement needle head with straight needles for MiniVap/ Ultravap™ 24
1
229409
Replacement needle head with spiral needles for MiniVap/ Ultravap™ 96
1
229072
Replacement needle head with straight needles for MiniVap/ Ultravap™ 96
1
229036
Replacement needle head with straight needles for Ultravap™ 384
1
229073
Spare needle kit, spiral. Set of 10 c/w fitting tool for MiniVap/Ultravap™ 96
1
229074
Spare needle kit, straight. Set of 10 c/w fitting tool for Ultravap™ 384
1
229075
Spare needle kit, straight. Set of 10 c/w fitting tool for Ultravap™ 96
1
229035
Gas filter, 5µm for MiniVap/Ultravap™
1
229088
Gasket for needle manifold for MiniVap/Ultravap™
1
229048
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
Spares and consumables for Porvair evaporators
AUTOM ATION ACCESSORIES
47
UltraVap 229135
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
E Q U I P M E N T – H E AT S E A L E R S
AUTOM ATION ACCESSORIES
48
Thermal plate sealers
Of the various methods for sealing
microplates, heat sealing has become the
preferred option. It creates an air tight and
chemically-resistant seal without the
complications of adhesives being applied to a
plate. Porvair Sciences has developed a range
of units to meet the needs of low, medium
and high throughput laboratories for
microplate sealing.
MicroSeal Manual
Thermal Plate Sealer
The MicroSeal is designed to be
compact, easy to use and
ergonomic. Heat sealing a wide
range of plates is quick and
simple. Heat sealing protects
precious samples from
evaporation and contamination
when performing PCR or during
storage. A built-in thermostat
prevents overheating of the
MicroSeal and with its small
footprint and ease of use, the new
MicroSeal is the obvious choice for
manual sealing of PCR plates and
microplates. With a pre-set temperature
of 170C, ideal for most common sealing
applications, and dual LED status display for
power and heating, the MicroSeal is safe and
easy to use. An ergonomic pull down action
allows single action sealing of most common
plate types. Additional adaptors are available
for specific plates.
●
Fast warm up time
●
Consistent, single action sealing
●
Pre-set temperature for ease of use
●
Sealing Temperature 170°C
●
Warm up time < 10 minutes
MicroSeal plate sealer
CUSTOM M ANUFACTURE
Description
Qty/pack
Cat. no.
MicroSeal plate sealer 110/230v
1
229751
Plate adaptor for ANSI/SBS deep-well plates
1
229752
Plate adaptor for rimless 96-well PCR plates
1
229753
Plate adaptor for ANSI/SBS standard 14mm plates
1
229754
W W W. M I C R O P L A T E S . C O M
●
Compact footprint
●
No compressed air needed
●
Semi-automatic operation
●
Adjustable temperature and sealing
time
●
LED display of time, temperature
and status
●
Unique safety features
●
Portable, lightweight design
●
Dual voltage 110V/230V
Rapid heat-up, a real-time display of the sealing head
temperature, automatic plate height detection and pre-cut
sealing films combine to simplify plate sealing. The “drawer”
design ensures quiet operation and eliminates any health &
safety risks, whilst an energy saving mode protects the
environment. Full microprocessor control allows you to
programme the optimal sealing time and temperature for
each plate type in use, whilst preventing damage caused by
“over sealing”.
The MiniSeal Plus can be used with any standard SBS
footprint plate up to 48mm high and with most currently
available PCR plates. Adaptors may be required for operation
with certain types of plates. Sealing can be achieved with
plates formed from polystyrene, polypropylene and the new
COC / COP cyclo-olefin polymers.
Films come in a range of materials suitable for storage,
transport, temporary or long-term sealing and piercing.
Height of the MiniSeal Plus is just 310mm.
MiniSeal Plus automatic single plate sealer
Description
Qty/pack
Cat. no.
Miniseal Plus 110v / 230v
1
229670
PCR Plate Adapter & Sealing Frame
1
229671
Standard SBS plate Adapter
1
229672
Spare Sealing Frame (PCR)
1
229673
Spare weighted Platen
1
229674
Fuses (110V)
1 pack
229675
Fuses (230V)
1 pack
229676
W W W. M I C R O P L A T E S . C O M
LIFE SCIENCES
SOLID PHASE EXTRACTION
Designed for laboratories needing to seal 20-40 plates per
day, the MiniSeal Plus is compact, easy to use and simple to
install. Unlike bigger automated sealers, MiniSeal Plus does
NOT require a compressed air supply to operate. Instead, all
operations are electronically controlled through the splashproof keypad, meaning the MiniSeal Plus can be installed, up
to temperature and sealing your plates within minutes of
unpacking. The compact footprint, about the size of a sheet
of A4 or letter paper, means MiniSeal Plus will fit just about
anywhere on your lab bench, or inside a fume hood if
required.
49
AUTOM ATION ACCESSORIES
The new MiniSeal Plus builds on the proven track record of
Porvair’s revolutionary MiniSeal semi-automatic microplate
sealer to bring you greater comfort and safety in use,
together with unmatched repeatability in sealing plates of
many different types and sizes.
CUSTOM M ANUFACTURE
MiniSeal Plus
semi-automatic heat sealer
STORAGE/COLLECTION
E Q U I P M E N T – H E AT S E A L E R S
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
E Q U I P M E N T – H E AT S E A L E R S
TriSeal™ heat sealer
TriSeal is an automated plate sealer that can
produce an accurate and tight seal on any
SBS-standard microplate up to a maximum
height of 45mm. The maximum obtainable
throughput is an impressive five plates per
minute. This is achieved using a unique
turntable design, which allows simultaneous
loading, unloading and sealing.
●
Automatically adjusts to plate height
●
Three position turntable design
●
Easy foil or film loading
●
Adjustable sealing temperature
from 50ºC up to 195ºC
●
Adjustable sealing time
from 1.0 to 30.0
seconds
●
Universal power
supply, no transformer
needed
●
Only 8 bar of compressed air needed
Description
●
Easy automation with stacker/loaders
or with LH robot via RS 232 port
●
Stand alone keypad for easy
operation and programming
●
CE marked
●
Built in self-test and fault diagnosis
software
AUTOM ATION ACCESSORIES
50
CUSTOM M ANUFACTURE
TriSeal
W W W. M I C R O P L A T E S . C O M
Qty/pack
Cat. no.
TriSeal heat sealer 110/220v
1
229400
Fully automatic TriSeal system with
Scorpion stacker/loader (right hand side)
1
Contact
Porvair
UHTS TriSeal system with two Scorpion
stacker/loaders (right & left hand sides)
1
Contact
Porvair
Scorpion stacker/loader 110/230v
1
229600
Scorpion/TriSeal integration plate
1
229605
STORAGE/COLLECTION
E Q U I P M E N T – M AT C A P P E R A N D D E - C A P P E R
Mat Capper
Caps shallow and deep well
polypropylene storage plates
●
Needs minimal pressure for capping,
reducing fatigue and RSI
●
Powder coated to resist chemical
spillage
●
Universal plate ‘shuttle’ to take
shallow or deep well plates
●
Fixing holes for securing to bench
Mat Capper
SOLID PHASE EXTRACTION
●
LIFE SCIENCES
For laboratories having to seal medium
numbers of microplates the Mat Capper
offers an affordable solution. Compact and
portable, the Mat Capper is very easy to use,
requiring only one operation of the system to
produce an accurate and tight seal on a wide
range of both collection and shallow well
microplates. The seals used are EVA or silicone
mats, which work by friction fit and are
re-usable with care. For a wide range of
friction seals, please see page 56.
MicroLute™ De-capper
The MicroLute™ De-capper is for use with
any MicroLute™ plate fitted with a drain cap.
It enables the quick and easy removal of the
protective lower drain cap, enabling the plate
to be used.
Description
Qty/pack
Cat. no.
Mat Capper, applicator for storage and assay plates
1
229078
MicroLute™ De-capper, for use with drain caps
1
229110
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
Mat Capper and MicroLute™ De-capper
AUTOM ATION ACCESSORIES
51
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
EQUIPMENT – ADHESIVE-SEAL SELECTION GUIDE
Application
Cell Culture
Microbiology
Fluorescence
Photoprotection
ELISA
General
Incubation
Protein
Crystallisation
Studies
AeraSeal
229307
229308
AbsorbMax
229520
Pierceability
required?
ThermalSeal RT
229301
229302
≥ -40˚C
Microplate
Adhesive-Seal
Selection Guide
No
SealPlate
229016
Robotics
Pierceability
Real-time?
Avoid
adhesive
fouling?
≥ -80˚C
Yes
AlumaSeal II
229305
No
EZ-Pierce
229306
ThermalSeal
229303
229304
MicroBurst
229731
Yes
ThermalSeal RT2RR
229521
Raised rim?
Pierceable?
No
Yes
ThermalSeal
229303
229304
Raised rim?
Yes
No
EZ-Pierce
229306
Protection?
No
52
AUTOM ATION ACCESSORIES
Yes
PCR
AlumaSeal CS
229499
Raised rim?
CUSTOM M ANUFACTURE
High
Throughput
Screening
Cold Storage
ThermalSeal RT
229301
229302
MicroBurst
229731
MicroBurst
229731
Yes
Short term
Long term
X-Pierce
229496
229495
Zone Free
229494
229493
No
Sealing Film Plus (blue)
229711
229712
229713
AlumaSeal 96
229497
AlumaSeal II
229305
AlumaSeal 384
229498
Sealing Film Plus (white)
229721
229722
Sealing Film Plus (blue)
229701
229702
229703
W W W. M I C R O P L A T E S . C O M
Porvair Sciences has a comprehensive range
of seals and foils for thermal and adhesive
sealing of microplates. In addition, a full range
of re-usable cap mats is available for friction
sealing of plates.
Foils and seals for the MicroSeal and MiniSeal
Plus are supplied as cut sheets, 125mm x
78mm, in packs of 100. For the automated
TriSeal system, these come in roll form.
Sample rolls are 5m long, whilst production
rolls are either 500m or 610m long, enough
to seal 5-6,000 plates on average.
All Porvair heat seals have a sealing integrity
range of -80ºC to +120ºC and are resistant to
DMSO and TFA as well as most other
solvents.
Description
Qty/pack
Cat. no.
All foils and films are 78mm width, sheet length is 125mm
Peel-Seal peelable sealing foil
1 x 610m
1 x 5m
229420
229450
Pierce-Seal pierceable sealing foil
1 x 610m
1 x 5m
229421
229451
Clear Seal optically clear film for
fluorescence/colorimetric assays
1 x 500m
1 x 5m
100 sheets
229422
229452
229472
Pierce-Seal easily pierced foil for PCR
1 x 610m
1 x 5m
229440
229460
Thermal bond strong peelable sealing foil
1 x 500m
1 x 5m
229442
229462
Clear Weld-Seal strong permanent seal
1 x 610m
1 x 5m
100 sheets
229441
229461
229481
Gas-permeable Rayon seal for cell culture
1 x 200m
1 x 5m
100 sheets
229443
229463
229553
Porvair colour-coded thermal seals
Description
Qty/pack
Cat. no.
100
229572
100
229571
100
229573
100
229574
Qty/pack
Cat. no.
SealPlate® clear adhesive film for PP or PS plates
100
229016
ThermalSeal RT2™ thermal cycler film for real-time PCR polyester 50µm thickness
100
229301
Easily pierced 20µm PP/PS lacquered aluminium foil – colour coded green
125mm x 78mm sheets printed with colour coding and Right Side Up for ease of use
Peelable 70µm polyester/alumninium laminate sealing foil – colour coded red
125mm x 78mm sheets printed with colour coding and Right Side Up for ease of use
LIFE SCIENCES
Heat seals for use with TriSeal or other automated sealers
SOLID PHASE EXTRACTION
Porvair seals and foils
STORAGE/COLLECTION
EQUIPMENT – SEALS AND FOILS
Pierceable/Peelable 38µm lacquered aluminium foil – colour coded blue
125mm x 78mm sheets printed with colour coding and Right Side Up for ease of use
Strong 85µm thick aluminium foil for long term storage – colour coded black
125mm x 78mm sheets printed with colour coding and Right Side Up for ease of use
Adhesive seals (suitable for use down to -40ºC unless shown)
100
229302
100
229303
ThermalSeal A® thermal cycler film for standard PCR, sterile
100
229304
ThermalSeal RT2RR™ thermal cycler film for real-time PCR – 50µm films
100
229521
AlumaSeal II™ aluminium seal for PCR and storage down to -40˚C
100
229305
AlumaSeal 96® aluminium seal for PCR and storage in 96-well plates with raised rims
100
229497
AlumaSeal 384™ aluminium seal for PCR and storage in 384-well rimless plates
100
229498
AlumaSeal CS™ aluminium seal for long term cold storage -80˚C
100
229499
Absorb Max™ optically-black film for light-sensitive assays
100
229520
EZ-Pierce™ easily pierceable polyethylene film
100
229306
Aeraseal™ gas-permeable, hydrophobic Rayon seal for cell culture (-20ºC only)
100
229307
Aeraseal™ gas-permeable, hydrophobic Rayon seal for cell culture, sterile (-20ºC only)
50
229308
X-pierce™ pre-scored film 96 Well Round – sterile
50
229495
X-pierce™ pre-scored film 96 Well Round
100
229496
W W W. M I C R O P L A T E S . C O M
AUTOM ATION ACCESSORIES
ThermalSeal RT5™ thermal cycler film for real-time PCR polyester 125µm thickness
ThermalSeal A® thermal cycler film for standard PCR
53
CUSTOM M ANUFACTURE
Description
STORAGE/COLLECTION
EQUIPMENT – SEALS AND FOILS
Porvair MicroBurst™
Microencapsulated clear sealing
film
SOLID PHASE EXTRACTION
LIFE SCIENCES
Using adhesive seals whilst wearing laboratory gloves can prove to be
difficult. The glued surface of the seal can easily stick to your glove
rather than your microplate. Porvair MicroBurst™ films eliminate this
problem. When first handled, MicroBurst™ is not tacky and will not
stick to anything. Only when you are satisfied that the film is in the
correct place do you press down hard to activate the MicroBurst™
adhesive. Millions of tiny spheroids contain the adhesive and these
“microcapsules” burst under applied pressure to release the adhesive
just where you want it and not all over your gloves!
The patented MicroBurst™ technology is being offered for the first time
in a Life Science laboratory by Porvair Sciences. The clear film has
excellent optical properties and once activated, produces a very firm
seal which quickly strengths to form a near-permanent seal on your
plates. MicroBurst™ film can be pierced by pipette tips or robot liquid
handlers and is temperature stable – making it ideal for PCR work.
MicroBurst™ non-sticky adhesive seal
Description
Qty/Pack
Cat. No.
100
229731
Adhesive film application paddle
5
229732
Adhesive film application roller
1
229733
Microburst™ clear seal with microencapsulated adhesive coating
Adhesive seals
229494
229016
229711
229712
229713
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
54
Zone-Free™ & well area adhesive-free films
Description
Qty/pack
Cat. no.
EZ-Pierce™ sealing film 96 Well Round – sterile
50
229493
EZ-Pierce™ sealing film 96 Well Round
50
229494
Sealing Film Plus – Blue Adhesive Polypropylene 100um 96 Well Square
100
229701
Sealing Film Plus – Blue Adhesive Polypropylene 100um 384 Well Square
100
229702
Sealing Film HT Autoclavable White Adhesive PTFE 50um 96 Square
100
229721
Sealing Film HT Autoclavable White Adhesive PTFE 50um 96 Well Round
100
229722
Sealing Film Plus – Blue Adhesive Polypropylene 100um 96 Well Round
100
229703
Sealing Film Plus – Blue Adhesive PTFE 50um 96 Well Round
100
229713
Sealing Film Plus – Blue Adhesive PTFE 50um 96 Well Square
100
229711
Sealing Film Plus – Blue Adhesive PTFE 50um 384 Well Square
100
229712
W W W. M I C R O P L A T E S . C O M
Heat seals and foils
Heat seals and foils
229472
LIFE SCIENCES
229443
STORAGE/COLLECTION
EQUIPMENT – SEALS AND FOILS
229481
229573
SOLID PHASE EXTRACTION
229574
229572
229571
286113
360005
286116
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
Friction sealing strips and PCR cap mats
AUTOM ATION ACCESSORIES
55
STORAGE/COLLECTION
EQUIPMENT – FRICTION SEALS
Friction seals
229090
229093
SOLID PHASE EXTRACTION
LIFE SCIENCES
219003
219004
360006
229091
Friction seals (cap mats)
Description
Silicone sealing mat to fit 384-well plates
AUTOM ATION ACCESSORIES
56
58
219040, 219041
50
380001
EVA sealing mat to fit 96-square well plates
219006, 219008, 219009
50
219004
EVA sealing mat to fit 96-square well plates, sterile
219025, 219026, 219027
50
219019
Pierceable Santoprene mat for 96-square well plates
219006, 219008, 219009
100
360010
EVA sealing mat for 96-round well plates
219002, 219032
50
219036
EVA sealing mat for 96-round well plates, black
219002, 219032
50
219038
EVA sealing mat for 96-round well plates, sterile
219012
50
219042
Pierceable Santoprene seal strip to fit 24-well plates
360013
50
360014
Pierceable Santoprene seal mat to fit 48-well plates
360002, 360004
100
360006
Pierceable Santoprene seal strip to fit 96-well plates
360001
400
360007
Pierceable sealing cap, round 96-well
219002
50
219044
Mat for round 96-well plates (standard silicone)
DNase/ RNase free, individually packed
50
229090
Mat for square 96-well plates (standard silicone)
229230 and square plates
5
229091
Mat for round 96-well plate (PTFE coated silicone)
5
229092
Mat for square 96-well plate (PTFE coated silicone)
5
229093
Polystyrene clear lid for all 96-well SBS standard plates
Fits all plates
100
229125
Polypropylene lid for all 96-well SBS standard plates
Fits all plates
100
229128
Cap mat for 1.1ml low profile storage plate
CUSTOM M ANUFACTURE
Cross reference to Porvair Sciences plates Qty/pack Cat. no.
5
219251
96-well PCR sealing mat, Natural
50
286113
96-well PCR sealing mat, Rubber, Natural
50
286114
1250
286116
8-well PCR sealing strip, 125 strips per pack, 10 packs per case
W W W. M I C R O P L A T E S . C O M
219250
The MicroShake™ orbital shaker is specially designed for use
with Porvair Sciences’ MicroLute™ P3 protein precipitation
plates. To ensure rapid and complete mixing of serum samples
with acetonitrile, simply place the MicroLute™ P3 plate on
the dedicated plate holder and agitate for up to 30 seconds.
The MicroShake™ has been tested with our P3 plates to
ensure that no breakthrough of supernatant liquid can occur
and has been shown to be the fastest way to ensure protein
precipitation throughout the sample. MicroShake™ uses a 15
volt power supply and is fitted with a speed adjuster. This
compact and quiet unit is barely bigger than a standard SBS
microplate and will fit on any bench, in any fume hood or
even the deck of your liquid handler. For added peace of
mind and to eliminate any risk of contamination, the P3 plates
can be fitted with a re-usable silicone cap mat. A simple
adjustment enables the MicroShake™ to be used with any
SBS footprint microplate.
LIFE SCIENCES
The latest addition to the Porvair Sciences
range of microplate equipment is this handy
single-rack microplate thawing station.
Ambient air is continuously and gently
circulated across the base of the frozen
microplate or tube rack, ensuring gentle, even
thawing without the risk of damage to your
precious samples. Economical, quiet in use and
boasting a small footprint on the bench, the
MicroStream™ is an ideal addition to your
microplate or tube-rack sample management.
MicroShake™ orbital shaker
SOLID PHASE EXTRACTION
MicroStream™ tube rack
and microplate thawing
station
STORAGE/COLLECTION
E Q U I P M E N T – M I C R O P L AT E T H AW I N G S TAT I O N
Description
Qty/pack
Cat. no.
1
229510
Qty/pack
Cat. no.
MicroShake™ variable speed orbital SBS microplate shaker 110V US plug
1
229651
MicroShake™ variable speed orbital SBS microplate shaker 230V UK plug
1
229652
MicroShake™ variable speed orbital SBS microplate shaker 230V EU plug
1
229653
MicroLute™ SPE & MicroLute™ P3 plate adaptor for MicroShake™
1
229654
Optional clear lid for MicroShake™ for use with 14mm high SBS microplates
1
229655
MicroStream™ microplate thawing station 110/220v
MicroShake™ orbital microplate shaker
Description
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
MicroStream™ microplate thawing station
AUTOM ATION ACCESSORIES
57
Reservoir trays
Porvair Sciences reservoir trays are designed
for use with any robotic liquid handling
system. These reservoirs offer options for a
single liquid or a partitioned space for several
liquids. Most working configurations are
accounted for as well as varying liquid
volumes. Made to take a range of liquid
handling configurations from 8 or 12 channel
pipettes through to 96 or 384 pipette heads.
●
Pyramid bottom for very low dead volume
●
Robot friendly
●
Moulded in natural virgin polypropylene
●
Chemically and heat resistant
●
Autoclavable
●
Can be gamma irradiated
●
Made to SBS/ANSI standard dimensions
EQUIPMENT
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
A U T O M AT I O N A C C E S S O R I E S – R E S E RV O I R T R AY S
CUSTOM M ANUFACTURE
58
Reservoirs
Description
Max. vol.
Dead vol.
Qty/Pack
Cat. no.
96-well, full height, pyramid bottom
300ml
<64µl
25
390001
12-column full height, pyramid bottom
290ml
<540µl
25
390002
8-row, full height, pyramid bottom
300ml
<82µl
25
390003
96-well, low profile, pyramid bottom
86ml
<64µl
25
390004
12-column, each of 21ml, partitioned, pyramid bottom
252ml
<54µl (per col)
25
390005
8-row reservoir, each of 32ml, partitioned, pyramid bottom
256ml
<82µl
25
390006
384-well, full height, pyramid bottom
282ml
<7µl
25
390007
384-well, low profile, pyramid bottom
92ml
<7µl
25
390008
Reservoir, low profile, flat bottom
85ml
–
25
390009
12-column, each of 7ml, partitioned, pyramid bottom, low profile
84ml
<54µl (per col)
25
390012
49.3ml
<120µl
25
390013
8-row, partitioned, each of 10.6ml, pyramid bottom, low profile
84ml
<82µl (per col)
25
390014
6-column, each of 47ml, partitioned, full height, pyramid bottom
282ml
<82µl (per col)
25
390015
384-well, 2 control wells, full height, pyramid bottom
282ml
<7µl
25
390016
16-row, partitioned, each of 4.9ml, low profile, pyramid bottom
78ml
<18µl (per col)
25
390017
24-column, each of 3.5ml, partitioned, low profile reservoir, pyramid bottom
84ml
<110µl (per col)
25
390018
4-column partition reservoir pyramid bottom
300ml
<540µl
25
390107
24-column reservoir, 3.5ml, partitioned, low profile
78ml
250µl
25
390108
384-well reservoir, 4 controls, half height
155ml
7µl
25
390109
384-well reservoir, controls, low profile, wave breakers
92ml
7µl
25
390110
4 rows of 73ml partitioned Reservoir, pyramid bottom
292ml
0.5ml
25
390111
Universal anti-splash reservoir baffle to fit 390009
–
–
10
390011
Disposable PVC trays for use with Porvair manifolds
–
–
25
219010
Polystyrene clear lid for all 96-well SBS standard plates
–
–
100
229125
Polypropylene lid for all 96-well SBS standard plates
–
–
100
229128
384-well, low profile, with four controls
W W W. M I C R O P L A T E S . C O M
Reservoir trays
390001
SOLID PHASE EXTRACTION
390003
LIFE SCIENCES
STORAGE/COLLECTION
A U T O M AT I O N A C C E S S O R I E S – R E S E RV O I R T R AY S
390004
EQUIPMENT
390006
390002
390011
390009
390007
390008
W W W. M I C R O P L A T E S . C O M
59
CUSTOM M ANUFACTURE
390005
STORAGE/COLLECTION
A U T O M AT I O N A C C E S S O R I E S – R E S E RV O I R T R AY S
Reservoir trays
EQUIPMENT
SOLID PHASE EXTRACTION
LIFE SCIENCES
390014
390013
390018
390012
390107
CUSTOM M ANUFACTURE
60
390015
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION
A U T O M AT I O N A C C E S S O R I E S – R E S E RV O I R T R AY S
Reservoir trays
390109
390108
390111
EQUIPMENT
390017
SOLID PHASE EXTRACTION
LIFE SCIENCES
219010
61
W W W. M I C R O P L A T E S . C O M
CUSTOM M ANUFACTURE
390110
EQUIPMENT
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
A U T O M AT I O N A C C E S S O R I E S – R E S E RV O I R T R AY S
Reagent Reservoirs for manual use
Following the outstanding success of Porvair polypropylene reagent reservoirs for automation, we are now
pleased to introduce a range of affordable, disposable polystyrene reservoirs with lid option for reagent
protection on the bench, together with re-usable reservoir holders with disposable polypropylene liners to
minimise waste. Our new range includes the innovative CoolTrough reservoir holder with built-in coolant to
keep reagents cool during use.
Disposable polystyrene reagent reservoirs with polystyrene lid option
Disposable reservoirs are available in a choice of volume ranges, 25-50ml and 50-100ml, and layouts in either
8- or 12- channel formats combined with a choice of sterile or non-sterile. Porvair’s affordable disposable
reservoirs with optional clear lids prevent contamination and also prevent accidental spillage when moving
reservoirs on the bench. In addition, the lids enable reservoirs to be stacked. Accurate pipetting is ensured by a
ridge on the side of the reservoir which helps the user to remove drops from the pipette tips. In addition, the
non- nesting design of Porvair’s reservoir trays makes it easy to separate a single reservoir from a pile.
Introducing CoolTrough cooled reagent reservoirs
To minimise waste in your laboratory and to help you maintain the cool chain right to your benchtop, Porvair
have introduced a new range of chilled reusable reservoirs with disposable polypropylene liners. These
CoolTrough Reagent Reservoir holders are designed to keep reagents cold during sample preparation (between
0-5 °C). As the rigid CoolTrough accepts disposable liners, it is very easy to change or replenish reagents during
a prep. With cold-gel sealed bases, CoolTrough holders can be stored in a freezer, removed prior to use and
fitted with a disposable polypropylene liner, making them very easy to use. Where cooling is not required,
Porvair offer reagent holders for use with the same disposable liners at ambient temperatures.
Reagent Reservoirs Manual / Bench Use
Description
Qty/Pack
Cat. no.
Reservoir lids 25 - 50ml. 10/pack, 50/case
50
386001
Reservoir lids 50 - 100ml and 8/12 channel reservoirs. 10/pack, 50/case
10
386002
25 - 50ml Reagent reservoir. Bulk Pack. Sterile, 10/pack, 200/case
200
386003
25 - 50ml Reagent reservoir. Sterile with lid. Individually wrapped. 100/case
100
386004
50 - 100ml Reagent reservoir. Sterile with lid. Individually wrapped. 100/case
100
386006
50 - 100ml Reagent reservoir. Sterile, bulk packed in bags of 10. 200/case
200
386005
100ml reagent troughs. Bulk pack of 100 non-sterile liners. One free re-useable holder included with every pack
1
386007
Pack of five re-usable holders for use with the 56-1001 reagent reservoir liners
5
386008
CoolTrough. Pack of five re-usable holders with cold-gel bases to keep reagents cool while on the bench.
5
386009
8 channel Reagent reservoir. Sterile with lid. Individually wrapped. 100/case
100
386010
8 channel Reagent reservoir. Sterile, bulk packed in bags of 10. 100/case
100
386011
12 channel Reagent reservoir. Sterile with lid. Individually wrapped. 100/case
100
386012
12 channel Reagent reservoir. Sterile, bulk packed in bags of 10. 100/case
100
386013
Texan™ reservoir with lid, graduated, non-sterile 175ml
10
229522
Texan™ reservoir with lid, graduated, Sterile 175ml
10
229527
8 & 12 Channel Reagent Reservoirs
Multi channel pipette reservoir polypropylene lidded
CUSTOM M ANUFACTURE
62
W W W. M I C R O P L A T E S . C O M
STORAGE/COLLECTION
A U T O M AT I O N A C C E S S O R I E S – R E S E RV O I R T R AY S
386005
386007
386009
229522
229527
EQUIPMENT
386003
SOLID PHASE EXTRACTION
LIFE SCIENCES
Disposable reservoir trays
386010
W W W. M I C R O P L A T E S . C O M
386012
CUSTOM M ANUFACTURE
63
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
CUSTO M M ANUFACTURE
Custom manufacture
Porvair Sciences Ltd has a long history of
successfully helping customers develop new
and innovative microtitre plates and
equipment. Porvair Sciences expertise in
polymer moulding, surface treatment,
specialist assembly and general understanding
of life science applications makes the
company ideal for custom manufacture. We
can also undertake ‘private labelling’ of our
standard products. Current customers for
custom manufacture include the following
groups:
●
Scientific instrument companies
●
Pharmaceutical R&D laboratories
●
Cell biology companies
●
Compound library suppliers
●
Diagnostic companies
●
Military contractors
●
Microarray research companies
AUTOM ATION ACCESSORIES
EQUIPMENT
Techniques which Porvair Sciences use in the
generation of custom products include:
●
Polymer ultrasonic welding
●
Plasma surface treatment of
polymers
●
‘Two-shot’ injection moulding
●
Co-sintering of polymers/silicas
Porvair Sciences offers rapid solutions to
problems by consultancy, design and speedy
prototyping for biotech, pharmaceutical and
life science companies.
W W W. M I C R O P L A T E S . C O M
64
PAGE
CODE
PAGE
CODE
PAGE
CODE
PAGE
CODE
PAGE
204003
21
219027
6
223109
23
229138
46
229496
53
229733
54
242020
38
204006
21
219036
56
223503
23
229139
46
229497
53
229751
48
242021
38
204012
21
219037
7
223509
23
229200
45
229498
53
229752
48
242022
38
204503
21
219038
56
224001
10
229201
45
229499
53
229753
48
242023
38
204512
21
219040
10
228001
43
229202
45
229510
57
229754
48
242024
38
205003
21
219041
10
228007
43
229203
45
229520
53
230001
32
242025
38
205006
21
219042
56
228008
43
229204
45
229521
53
230002
32
242026
38
205012
21
219044
56
228009
43
229205
45
229522
62
230003
32
242027
38
205503
21
219101
35
228010
43
229230
9
229527
62
230004
32
244001
38
205512
21
219102
35
228012
43
229301
53
229553
53
239007
38
244002
38
208003
8
219103
35
228018
11
229302
53
229571
53
239010
38
244003
38
208004
20
219115
35
228019
11
229303
53
229572
53
240002
38
244004
38
209003
8
219250
9
228020
44
229304
53
229573
53
240010
40
244005
38
209004
20
219251
9&56
228021
44
229305
53
229574
53
240011
38
244006
38
210003
8
219412
7
228022
44
229306
53
229600
50
240030
42
244007
38
210004
20
219422
7
229016
53
229307
53
229605
50
240031
42
244008
38
214003
25
219432
7
229035
47
229308
53
229651
57
240054
38
244009
38
214006
25
221003
23
229036
47
229400
50
229652
57
240100
40
244010
38
214030
25
221006
23
229048
47
229409
47
229653
57
240200
40
244011
38
214503
25
221009
23
229070
46
229420
53
229654
57
242001
38
244012
38
214506
25
221012
23
229071
46
229421
53
229655
57
242002
38
244013
38
215003
25
221103
23
229072
47
229422
53
229670
49
242003
38
244014
38
215006
25
221109
23
229073
47
229440
53
229671
49
242004
38
244015
38
215030
25
221503
23
229074
47
229441
53
229672
49
242005
38
244016
38
215503
25
221509
23
229075
47
229442
53
229673
49
242006
38
244017
38
215506
25
222003
23
229078
51
229443
53
229674
49
242007
38
244018
38
219002
7
222006
23
229080
46
229450
53
229675
49
242008
38
244019
38
219004 41&56
222009
23
229081
46
229451
53
229676
49
242009
38
244020
38
219005
41
222012
23
229088
47
229452
53
229701
54
242010
38
244021
38
219006
6
222103
23
229090
56
229460
53
229702
54
242011
38
244022
38
219008
6
222109
23
229091
9&56
229461
53
229703
54
242012
38
244023
38
219009
6
222503
23
229092
56
229462
53
229711
54
242013
38
244024
38
219010
222509
23
229093
56
229463
53
229712
54
242014
38
244025
38
41,43&58
223003
23
229110
51
229472
53
229713
54
242015
38
244026
38
219012
7
223006
23
229125 56&58
229481
53
229721
54
242016
38
244027
38
219019
56
223009
23
229128 56&58
229493
54
229722
54
242017
38
246001
38
219025
6
223012
23
229135
46
229494
54
229731
54
242018
38
246002
38
219026
6
223103
23
229136
46
229495
53
229732
54
242019
38
246003
38
W W W. M I C R O P L A T E S . C O M
65
LIFE SCIENCES
CODE
SOLID PHASE EXTRACTION
PAGE
EQUIPMENT
CODE
AUTOM ATION ACCESSORIES
PAGE
CUSTOM M ANUFACTURE
CODE
STORAGE/COLLECTION
INDEX BY NUMBER
CUSTOM M ANUFACTURE
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
LIFE SCIENCES
STORAGE/COLLECTION
INDEX BY NUMBER
CODE
PAGE
CODE
PAGE
CODE
PAGE
CODE
PAGE
CODE
PAGE
CODE
PAGE
CODE
PAGE
246004
38
248015
38
271022
41
303006
24
360006
56
360048
32
380001
56
246005
38
248016
38
271023
41
303008
24
360007
56
360049
32
386001
62
246006
38
248017
38
271024
41
303010
24
360008
32
360050
32
386002
62
246007
38
248018
38
271026
41
303012
24
360010
56
360051
32
386003
62
246008
38
248019
38
271028
41
311001
26
360011
33
360052
32
386004
62
246009
38
248020
38
271030
41
311003
26
360013
11
360053
32
386005
62
246010
38
248021
38
271032
41
311030
26
360014
56
360055
33
386006
62
246011
38
248022
38
286101
13
311503
26
360016
32
360056
33
386007
62
246012
38
248023
38
286102
13
312001
26
360017
32
360057
33
386008
62
246013
38
248024
38
286103
13
312003
26
360019
33
360058
33
386009
62
246014
38
248025
38
286104
13
312030
26
360020
32
360059
33
386010
62
246015
38
248026
38
286105
13
312503
26
360021
33
360060
32
386011
62
246016
38
248027
38
286106
13
318001
39
360022
33
360061
32
386012
62
246017
38
250001
38
286107
13
318002
39
360023
33
360062
32
386013
62
246018
38
250002
38
286108
13
318003
39
360024
33
360063
33
390001
58
246019
38
250003
38
286109
13
318004
39
360025
33
360064
32
390002
58
246020
38
250004
38
286110
13
320001
39
360026
32
360065
33
390003
58
246021
38
250005
38
286111
13
320002
39
360027
32
360066
32
390004
58
246022
38
250006
38
286112
13
320003
39
360028
32
360067
33
390005
58
246023
38
250007
38
286113
56
320004
39
360029
33
360068
33
390006
58
246024
38
250008
38
286114
56
321001
39
360030
33
360069
33
390007
58
246025
38
250009
38
286115
13
321002
39
360031
33
360070
32
390008
58
246026
38
250010
38
286116
56
321003
39
360032
33
360071
33
390009
58
246027
38
250011
38
301002
27
321004
39
360033
33
360073
33
390011
58
248001
38
250012
38
301004
21
322001
39
360034
33
360075
33
390012
58
248002
38
250013
38
301006
27
322002
39
360035
32
360076
33
390013
58
248003
38
250014
38
301010
27
322003
39
360036
32
360077
11
390014
58
248004
38
250015
38
301012
27
322004
39
360037
32
360079
11
390015
58
248005
38
250016
38
301014
27
324001
29
360038
32
360080
11
390016
58
248006
38
250017
38
301018
27
324011
29
360039
32
360090
33
390017
58
248007
38
250018
38
301060
27
324021
29
360040
32
360105
33
390018
58
248008
38
250019
38
301070
27
324031
29
360041
32
360107
33
390107
58
248009
38
250024
38
301502
27
324041
28
360042
32
360108
33
390108
58
248010
38
250026
38
301506
27
324051
28
360043
32
360109
33
390109
58
248011
38
271014
41
301510
27
327001
30
360044
32
360110
33
390110
58
248012
38
271016
41
301512
27
327002
30
360045
32
360111
33
390111
58
248013
38
271018
41
303002
24
360002
11
360046
32
360114
33
248014
38
271020
41
303004
24
360004
11
360047
32
360115
11
66
W W W. M I C R O P L A T E S . C O M
Chemical compatibility chart
This chemical compatibility chart should be used as a general guide. It is recommended that tests are peformed if there is any doubt about
material compatibility
Plate matrix
Cellulose nitrate
Nylon
PVDF
Polystyrene
Polypropylene
Acids
Acetic acid 25%
Acetic acid 100%
Formic acid 25%
Formic acid 100%
Hydrochloric acid 25%
Hydrochloric acid 100%
Nitric acid 25%
Nitric acid 65%
Phosphoric acid 25%
Sulphuric acid 25%
Sulphuric acid 98%
S
X
S
M
S
X
S
X
S
S
X
S
M
X
X
X
X
?
X
?
?
X
S
?
S
S
S
S
S
M
S
S
X
S
M
M
M
S
M
X
X
M
S
X
S
?
S
S
S
S
S
S
S
S
S
Alcohols
Amyl alcohol
Benzyl alcohol
Ethanol (ethyl alcohol) 70%
Ethanol (ethyl alcohol) 98%
Ethylene glycol
Glycerol
Isopropanol, n-propanol
Methanol 98%
Phenol
Propylene glycol
S
M
M
X
M
S
S
X
S
X
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
M
S
S
X
M
M
S
S
S
M
M
S
S
S
S
S
S
S
S
S
S
S
Bases
Ammonium hydroxide 25%
Ammonium hydroxide 1N
6N Sodium hydroxide
Potassium hydroxide
Sodium hydroxide 5%
Sodium hydroxide 1N
X
S
X
X
X
X
S
S
?
?
S
S
M
S
X
S
S
S
M
M
S
M
M
M
S
S
S
S
S
S
Esters
Amyl, Propyl, Butyl acetate
Benzyl benzoate
Ethyl acetate, Methyl acetate
2-Ethoxyethyl acetate
Methyl cellosolve acetate
X
S
X
X
X
S
?
S
?
S
M
M
M
S
S
X
?
X
X
X
M
M
M
M
M
Hydrocarbons
Gasoline, Kerosene
Hexane
Toluene
Xylene
Carbon tetrachloride
Chloroform
Freon
Methylene chloride
Monochlorobenzene
Perchloroethylene
1,1,1-Trichlorethane
1,1,2-Trichlorethane
Trichloroethylene
S
S
S
S
S
S
S
M
S
S
M
X
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
X
X
X
X
X
X
X
X
X
X
X
X
M
M
M
M
M
M
M
M
M
M
X
X
M
Ketones
Acetone
Cyclohexanone
Methyl ethyl ketone
X
X
X
S
S
S
X
M
M
X
X
X
M
M
M
Miscellaneous
Acetronile
Dimethylsufloxide (DSMO)
Dioxane
Ethyl ether
Formaldehyde 30%
Hydrogen peroxide 30%
Methyl cellosolve
Pyridine
Tetrahydrofuran
X
X
X
M
S
S
X
X
X
S
?
S
S
?
S
S
M
S
S
X
X
S
S
S
S
S
X
X
M
X
X
X
S
X
X
X
S
S
S
S
S
S
S
M
S
AUTOM ATION ACCESSORIES
EQUIPMENT
SOLID PHASE EXTRACTION
LIFE SCIENCES
Chemicals
S = suitable M = short term contact X = unsuitable ? = not tested Tests were performed at room temperature
W W W. M I C R O P L A T E S . C O M
67
CUSTOM M ANUFACTURE
Membranes
STORAGE/COLLECTION
C H E M I C A L R E S I S TA N C E T O M E M B R A N E S A N D P L AT E M AT R I C E S
P/N 420001 © Porvair Sciences Limited 2010.
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