Gilson Glider Prep Software User's Manual

Gilson Glider Prep Software

User’s Guide

Gilson, Inc.

| PO Box 620027 | Middleton, WI 53562-0027 | Tel: 608-836-1551 OR 800-445-7661 | Fax: 608-831-4451

Gilson S.A.S.

| 19, avenue des Entrepreneurs | BP 145, F-95400 Villiers-le-bel, FRANCE

www.gilson.com

| [email protected] | [email protected] | [email protected]

LT380083-01

2

Table of Contents

1 Introduction

Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

System Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Title Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Status Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Menus and Toolbars . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Toolbar Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

File Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Open Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

File Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Print . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Print Preview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Print Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Recent Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Exit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Open Method Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Current Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Manual Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Sequence Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

File Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Numbered Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Open Data Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Predefined Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Setup Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

View Current Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Edit Current Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Table of Contents

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Edit Numbered Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

User Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

System Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Save Method Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Current Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

As Numbered Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

As File Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Control Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Collector > Next Tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Collector > To Waste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Collector > All . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Collector > Threshold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Collector > Waste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Collector > Automatic Gradient Optimization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Collector > Local Minimum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Pause . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Hold Gradient . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Resume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Purge Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33


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User’s Guide

3

View Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Gradient OR Pressure OR Flow Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Peak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Signal 1, 2, 3, OR 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Panel Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Default Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Log Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Login OR Logout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

User’s Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Help Menu (?) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

About Gilson Glider Prep . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Traces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Export Traces to USB Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

2 Interface Organization

Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Panels Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Tabbed Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Synoptic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Solvent Containers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Adding or Removing Solvent Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Percent of Solvents Pumped . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Timer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Virtual Keyboard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Flow Rate Indicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Pressure Indicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

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Backflush Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Column Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Column Switching . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Lamp Activation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Active Lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

Auto Zero Dialog Box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

Wavelength Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

3-Way Collection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Collection Tube Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Waste Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Peak Tracking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Collection Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Curves Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

Vertical Toolbar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

3 File Management

Presentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Wizard Description: List of Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Creating Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Copying Methods and Sequences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Saving Methods and Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Deleting Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62


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4 Methods

Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Creating Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Graphical Interface Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Method Panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

General Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

Settings Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Stop Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

Max Pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

ELSD Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Elution Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Automatic Gradient Optimization (AGO) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Injection Mode (Section 1) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Injection Mode (Section 2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Equilibration Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

Elution Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

Consumption of Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

Available Parameters for Equilibration and Elution Gradients . . . . . . . . . . . . . . . 72

Gradient Nodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

Backflush Valve Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

Curves Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

Zoom Out Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

Show/Hide Gradient Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

Add Step and Delete Step Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Show/Hide Cursor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Equilibration and Elution Toggle Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

6

Table of Contents

|


Tabular & Graphical Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

Slope vs . Step Toggle Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

Table vs . Graph Toggle Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

Add or Delete Node Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

Incremental Addition and Subtraction Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

Detection Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

UV600 Detector Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

UV600 Scan Detector Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

ELSD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

Collection Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Add and Delete Step Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

Method Runs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Configure the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Prepare the Containers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Fill and Empty Containers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Prime the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

Open a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

Run a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

Starting and Stopping a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

Sequence Edition and Starting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

Create a New Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

Wait Messages and Error Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

Wait Message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90


|

User’s Guide

7

5 Configurations

Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Offset Diagram and Legend . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

User Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

User Profile Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

Line Widths . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

Line Color . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

Advanced Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

Menus Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

Curves Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

Edit Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

Reports Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

Physical Units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

System Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

General Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106

Pump Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

Detector Panels (#1, #2, #3, etc .) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108

Collector Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109

Valves Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

Injection Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

Alarms Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115

8

Table of Contents

|


Introduction

Contents

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

System Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

File Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Open Method Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Open Data Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Setup Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Save Method Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Control Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

View Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Log Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Help Menu (?) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36


|

User’s Guide

9

Overview

This user’s guide provides instructions for using


, which can be used to control

PLC 2050/2250/2500 Purification Systems

with integrated touchscreens . The software can also

be installed on a computer . Refer to

User Profile on page 96

for more details .

The control screen shows the state of elements in the system and the evolution of parameters in real time .

To limit the number of buttons and provide shortcuts for users, some graphic objects on the screen remain active . For instance, touching or clicking a solvent bottle allows users to change the volume .

Figure 1: Gilson PLC System

10

Introduction

|


System Options


can be configured with various pump heads, rack types, or gradient options . Available options are provided below .

Flow Rate

● 4 .5 mm Pump Head (50 mL/min) (

PLC 2050

)

● 10 mm Pump Head (250 mL/min) (

PLC 2250

)

● 14 mm Pump Head (500 mL/min) (

PLC 2500

)

Pressure

● 4 .5 mm Pump Head (300 bar / 4 351 psi) (

PLC 2050

)

● 10 mm Pump Head (230 bar / 3 336 psi) (

PLC 2250

)

● 14 mm Pump Head (110 bar / 1 595 psi) (

PLC 2500

)

Gradient

● Binary or Quaternary

Detection

● UV-VIS, Dual-Wavelength Spectrophotometer

Collection

● Numerous rack types

Loop Injection Valve

● 6-Way, 2-Position Valve for 1/8" Tubing

Backflush Valve


Column Switching Valve



|

User’s Guide

11

User Interface

The control screen can display the

Current Method (1)

,

Synoptic Screen (2)

,

Results Plot (3)

,

Peak Collection Tracking (4)

,

Collection Table (5)

, and

Monitored Wavelength Selection (6)

. The

Current Method

appears above the results plot . The

Synoptic Screen

displays the system and its component parts . The bottom panel is dedicated to

Results Plotting

and allows users to graphically edit equilibration and elution steps with a toolbar and drag-and-drop curves . Some active icons and buttons are also used for method settings . Panel positions and sizes can be modified . The

Scan

feature allows users to acquire real time absorbance values for multiple wavelengths and plot the mean value .

2

4

5

1

3

6

Figure 2: User Interface

Title Bar

The

Title Bar

of the main screen (located on top of the window) contains the name of the application followed by the software version currently used . If a user is logged in, their login name appears in the title bar, but it disappears as soon as the user

Guest

is connected or the user logs out .

: Karen

Figure 3: Title Bar

12

Introduction

|


Status Bar

The

Status Bar

, located at the bottom of the screen, reports on the application’s state and gives indications on the software shortcuts .

Figure 4: Status Bar

Menus and Toolbars

The menu bar contains the software functions, which are activated by mouse or stylus (for the touchscreen) .

F ile

M enu

The

File Menu

allows users to access to open files (

Open Data

), manage files (

File Management

), print methods and results (

Print

), preview print jobs (

Print Preview

), adjust print options (

Print Setup

), or

Exit

the program .

Figure 5: File Menu

S etup

M enu

The

Setup Menu

allows users to

View Current Method

,

Edit Current Method

,

Edit Numbered Method

,

Edit File Method

, open

Sequence

files, or adjust parameters for

Bottles

,

Columns

,

Racks

, and

Solvent

bottles . The

User Profile

and

System Configuration

tool are also accessible .

Figure 6: Setup Menu


|

User’s Guide

13

C ontrol

M enu

The

Control Menu

allows users to access

Collector

control options, including

Next Tube

,

To Waste

,

All

,

Threshold

,

Waste

,

Automatic Gradient Optimization

, and

Local Minimum

. The menu also allows users to

Run

,

Start

,

Pause

,

Hold Gradient

,

Resume

, or

Stop

the method .

Figure 7: Control Menu

V iew

M enu

The View Menu provides

Language

options for English, Deutsch, Français, Italiano, or Español . Select

Gradient

,

Pressure

,

Flow Rate

,

Peak, Signal

, or

Threshold

to toggle a check box to make those elements appear or disappear from the main screen . Select

Synoptic

,

Peak Tracking

,

Collection Table

, or

Spectra

to toggle visibility for these windows . Select

Default Layout

to reset the windows to the default layout . Select

Save Layout

to create a new default layout file .

14

Figure 8: View Menu

Introduction

|


l og

M enu

The

Log Menu

allows users to

Login

or

Logout

of user accounts . Users can also view their

Audit

trail, a list of stored users in the

Users Table

, stored profiles in the

Profiles Table

, and screens in the

Screens Table

.

Figure 9: View Menu

H elp

(?) M enu

The

Help (?) Menu

allows users to access contact information and input license codes

About Gilson Glider Prep

, activate

Traces

,

Export Traces to USB Key

, and view component

Information

and

Licenses

.

Figure 10: Help Menu


|

User’s Guide

15

Toolbar Buttons

The toolbar consists of buttons used for method edition, configuration, and control of the system . Some buttons are shortcuts to menu functions .

1 2 3 4 5

Figure 11: Toolbar Buttons 1 to 5

1 . Open Data

2 . Open Method

3 . Save Method

4 . View Method

5 . File Management

6 7 8

9

10 11 12

Figure 12: Toolbar Buttons 6 through 12

6 . Purge

7 . Run

8 . Start

9 . Pause

10 . Hold Gradient

11 . Resume

12 . Stop

16

Introduction

|


13 14 15 16


13 . Synoptic

14 . Peak Tracking

15 . Collection Table

16 . Default Layout

17 18 19

20 21 22

17 . Next

18 . Waste


19 . Collect All

20 . Threshold Collect

21 . Automatic Gradient Optimization

22 . Local Minimum


|

User’s Guide

17

File Menu

Figure 15: File Menu Location

Open Data

Opens the results of a method already executed and saved as a

(.prep)

file . This contains the method itself, the chromatogram, and its associated table .

Figure 16: Open Data Window

File Management

Opens the wizard for methods and data files management . Users may

Create

,

Edit

,

Copy

,

Save

, or

Delete

files from this screen . For more details, see

File Management on page 59

.

Figure 17: File Management Screen

18

Introduction

|


Print

Prints a report (after confirmation) containing all parameters of the last method used and the results obtained and currently displayed on the screen . The components visible on the report are configurable in the

User Profile

window . For more details see

Reports Components on page 104

.

Print Preview

Displays a screen to preview the results printout . Users can zoom in or out, display one or multiple pages, or directly launch the print screen (

Print

button) . Click

Close

to return to the user interface without printing .

Figure 18: Print Preview Screen


|

User’s Guide

19

Print Setup

Allows users to select the printer, change its properties, and select the paper orientation .

Figure 19: Print Setup Screen

Recent Files

Displays a list of the last

(.prep)

files opened .

Exit

A dialog box will appear . Select

OK

to close the application and

Cancel

to remain open . Always shut down the software before switching the instrument off .

Figure 20: Application Close Dialog Box

20

Introduction

|


Open Method Button

This button is only active when the system is stopped .

Current Method

Opens the current method displayed on the screen . Methods can be saved as numbered or standard method files for future use . Also accessible through

Setup > Edit

.

Figure 21: Editing a Method

Manual Method

If any method is present, this option opens a new, editable method .

Sequence Method

Opens a Windows Explorer screen where users can select a

Sequence File (.seq)

.


|

User’s Guide

21

File Method

Opens a Windows Explorer screen where users can select a

Method File (.lfh)

.

Figure 22: Opening a Method File

Numbered Method

Opens the

Numbered Method

window . Saved numbered files will appear as “raised” tiles and can be selected with a click . Available numbered methods will appear as gray . A preview of the method will appear for the method selected .

Figure 23: Numbered Method Window

22

Introduction

|


Open Data Button

Predefined Methods

Accessed by browsing to

Program Files > PrepWin v5.0 > Predefined

in Windows Explorer .

e xponential

S tandard

Figure 24: Exponential Standard Method

i

SoCratiC

S tandard

Figure 25: Isocratic Standard Method


|

User’s Guide

23

S tep

S tandard

Figure 26: Step Standard Method

l inear

S tandard

Figure 27: Linear Standard Method

24

Introduction

|


Setup Menu

Figure 46: Setup Menu Location

View Current Method

Opens the current method for viewing .

Edit Current Method

Opens the current method for editing .

Edit Numbered Method

Opens the numbered method window . Users can select a method from one of the 32 tiles to edit .

Figure 28: Numbered Method Windows

Sequence

Opens a sequence file stored, by default, to

C:/Program Files/Prep Win v5.0/Method

.


|

User’s Guide

25

Bottles

Adjustments can be made to the capacities of solvents and waste containers . Users can also change the names of solvent bottles .

Figure 29: Editing Bottle Capacities

Columns

Edits or adds columns used with the system .

Figure 30: Editing Columns

26

Introduction

|


Racks

Allows users to upload and edit a rack table after navigating a prompt screen .

Figure 31: Adding or Editing Racks

Solvents

Allows users to add or edit the solvents used in the system .

Figure 32: Adding or Editing Solvents


|

User’s Guide

27

User Profile

Allows users to create a custom design interface and configure all functions in the software, such as colors, display settings, parameters, etc .

Figure 33: User Profile Window


Opens a window to configure system components and the communication ports with the computer .


28

Introduction

|


Save Method Button

Only active when a method is currently displayed on the screen . This button allows users to save the current method before running it .

Current Method

Saves the current method . Active only if the current method is already stored as a numbered method or method file .

As Numbered Method

Saves the current method as a numbered method, which is selected from one of 32 numbered tiles .

Figure 35: Numbered Method Selection

As File Method

Saves current method file to the desired location .


|

User’s Guide

29

Control Menu

Figure 47: Control Menu Location

Collector > Next Tube

Flow to Waste

if the collection is stopped

● Starts (or restarts) the collection to the next tube

Flow to Needle

if the collection is running

● Changes collection tube

Collector > To Waste

Stops collection and sends liquid to

Waste

.

Collector > All

Forces a continuous collection into the rack tubes .

Collector > Threshold

Starts or restarts a collection mode adjusted to the current absorbance signal . When a signal is below the

Threshold

, the mobile phase flows to waste . When the signal is above the

Threshold

, the mobile phase is collected .

30

Introduction

|


Collector > Waste

Forces a continuous rejection to

Waste

, whatever the absorbance .

Collector > Automatic Gradient Optimization

Activates the

Automatic Gradient Optimization (AGO)

feature, which holds current gradient parameters when a peak appears on the chromatogram during separation .

Collector > Local Minimum

Activates the

Local Minimum

feature, which moves the probe to the next tube during a collection phase when an inflection of the signal appears on the chromatogram .

Run

Launches the current method .

Start

Starts the method at any time during the equilibration phase . This action is typically done automatically, such as after injection through loop .

1 . Removes the curves of the equilibration phase (grey background) .

2 . Autozeroes the detector channel .

3 . Asks for the injection confirmation (in case of

Wait for Inj.

mode) .

4 . Starts the plotting of the curve(s) (white background) .


|

User’s Guide

31

Pause

Stops the pump, but keeps the current parameters stored in memory .

● Pauses the method, time elapsed, and signal plots .

Hold Gradient

Holds the gradient in progress, but allows the pump to continue with current parameters .

● Curve plotting continues .

Resume

After a

Pause:

1 . Pump restarts .

2 . Resumes the method interrupted by

Pause

(time, gradients, signal acquisition, etc .) .

After a

Hold Gradient:

● Resumes the gradients fixed by

Hold Gradient.

Stop

Stops the method after a confirmation request .

Results of the method are automatically saved to the

Data Folder

with the date format .

● Curves and results remain on the screen until the next run .

32

Introduction

|


Purge Button

Active when the system is stopped or when a method is currently being edited on the main screen . This function allows the pump to prime without introducing air into the hydraulic circuit and the column . It can also be used to purge or rinse the hydraulic circuit .

If a method was in process when the

Purge

mode is started, the configuration and parameters of the method will return when the

Purge

mode has completed .

Selecting

Purge

then

Run

will prompt a confirmation window .

+

Figure 36: Purge Options and Run Button

After confirmation, the pump will start automatically . Manual control is possible via the synoptic window .

No curves will be plotted; however, the current absorbance values will be displayed . Once the

Purge Mode

has completed, another prompt will appear, instructing users to close the purge valve .

Instructions are identical for

Purge A 100%

,

Purge B 100%

, etc ., but the pump begins immediately without prompts and at the flow rate designated for the solvent .

Figure 37: Purge Valve Open Confirmation Prompt

Figure 38: Purge Valve Close Confirmation Prompt


|

User’s Guide

33

View Menu

Figure 39: View Menu Location

Gradient OR Pressure OR Flow Rate

Allows users to control the curves that are visible on the chart

● Checked = Displayed

● Unchecked = Masked

Peak

Allows users to change the appearance of

Peak Tracking

and curves panel to help visualize the tubes where the same peaks are collected .

● Checked = Activated

● Unchecked = Inactive (All tubes have a unique color)

Signal 1, 2, 3, OR 4

Allows users to select the signals that are visible on the curves panel .

Language

Allows users to select

Deutsch, English, Français, Italiano, Español

.

Panel Options

Shows or hides the panels selected:

Synoptic

,

Peak Tracking

,


, or

Spectra

.

Default Layout

Positions all panels according to the

Default Layout

.

34

Introduction

|


Log Menu

Figure 40: Log Menu Location

Login OR Logout

Opens a dialog box that allows users to identify themselves by selecting their login from a drop-down list and entering their password . Password characters are not viewable on the screen . Logins other than

Guest

allow users to save their personal settings .

Logout

closes the session for the current user .

Figure 41: User Login Window

User’s Table

Opens a window to add new users by providing a

Name

and

Password

.

Figure 42: User’s Table Login Window


|

User’s Guide

35

Help Menu (?)

Figure 43: Help Menu Location

About Gilson Glider Prep

● Displays information about the software .

● Provides access to special option activations . Activation keys are generated with an encrypted server via the 8 character code provided .

Traces

Enables the generation of trace files to debug anomalous behaviors . These files are automatically reset each time the software is opened .

● Checked = Activated

● Unchecked = Deactivated

Export Traces to USB Drive

Regroups the current trace files into a ZIP folder and copies it, in one click, to a USB drive (if present) . If no

USB drive is detected, the ZIP folder is copied to computer’s hard drive .

Figure 44: Trace to ZIP Dialog Box

36

Introduction

|


Information

Provides useful information for integrated devices, such as the firmware version, detector lamp life, etc .

Figure 45: Information Window


|

User’s Guide

37

Interface Organization

Contents

Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Synoptic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Peak Tracking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Collection Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Curves Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

Vertical Toolbar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58


|

User’s Guide

39

Overview

The user interface is composed of four windows used to control the system and observe results:

Synoptic

,


,

Peak Tracking

, and the

Curves Panel

. The main advantage of this interface is its modularity . Users can customize the panels as they choose .

Panels Layout p anel

d ragging

To move a panel, click on the title bar without releasing the stylus or mouse . Four square icons with arrows will appear . One cross with five icons appears in the center of the panel . When your cursor passes over another panel, the cross moves .

Figure 49: Directional Icons

Figure 50: Example of Panel Dragging

The

Curves Panel

has no title bar, so it is not moveable with panel dragging . Panels will be highlighted in blue to indicate the area the panel will occupy .

40

Interface Organization

|


t oolbar

d ragging

The toolbar is divided into four separate groups that can be moved individually to the locations shown below . Place the cursor over the dotted line on the left edge of a toolbar segment, then click and hold to drag .

Figure 51: Toolbar with Active Cursor

Figure 52: Available Slots to Move Toolbars


|

User’s Guide

41

Tabbed Interface

Every panel (except the

Curves Panel)

can be arranged into several tabbed windows . Activating a tab makes the associated content visible and the tab is highlighted to distinguish it from inactive tabs .

Figure 54: Three Panels in One Window

Figure 53: Two Panels in One Window with an Independent Synoptic Panel

To move a

Tabbed Panel

, click and hold the title bar and then drag it into place . As with other panels, the area the tabs will shift to will be highlighted in blue .

Figure 55: Moving Tabbed Panels with Active Areas Highlighted

p anel

r eduCtion and

e nlargeMent

Panels can be enlarged or reduced in size by placing the stylus or mouse on the borders in order to display the double arrow cursor . Click and drag and resize the windows horizontally or vertically .

Vertical Adjustment

Figure 56: Vertical Adjustment of Panels

42


|


Horizontal Adjustment

Figure 57: Horizontal Adjustment of Panels

p anel

M aSking and

d iSplay

Each panel (except the

Curves Panel

) can be masked or displayed on the user interface using the toolbar on the

View Menu

displayed below . When the icon background is orange, the panel is displayed . When the background is gray, the panel remains hidden . Close the panel by clicking on the

X

in the top right corner .

Synoptic Collection Table

Peak Tracking

Figure 58: Masking or Displaying Panels

a utoMatiC

r eorganization

Selecting the

Default Layout Button

automatically reorganizes the panels with

Synoptic

at the top left,

Peak Tracking

at the top center,


to the top right, and the

Curves Panel

below .

Window organization at the time of closing is saved and repeated upon the next software opening .


|

User’s Guide

43

e xaMpleS oF

o rganization

Figure 59: Examples of Panel Organization

44


|


Synoptic Screen

The

Synoptic Panel

shows the state of the system by means of graphic objects and numeric readout .

Some graphics are active and allow for real-time alteration of parameters .

Figure 60: Synoptic Panel

To change the parameters of active area, select the area and input values on the

Virtual Keypad

. There are three ways to alter the values displayed:

● Type values in keypad

● Use up or down arrows to increase or decrease values

● Drag the blue button up or down

Select

OK

to validate, and

Cancel

or the red

X

to close without applying .

Solvent Containers

Up to four solvent containers can be displayed with the remaining content given in milliliters .

Figure 61: Solvent Bottle Volume Selection


|

User’s Guide

45

Adding or Removing Solvent Bottles

A dialog box appears when the solvent bottle is selected . Users can enter the volume in milliliters and must select

OK

to confirm their choice . Selecting the area just above the solvent bottle allows users to add or subtract the number of solvent bottles used by checking or unchecking

Eluent A

,

Eluent B

, etc .

Figure 62: Adding or Removing Solvent Bottles

Percent of Solvents Pumped

The percentage of solvent pumped from each solvent bottle is indicated above the solvent bottles . Total percentage values must equal 100% . Users can change solvent proportions by selecting the rungs above the bottles .

Figure 63: Changing the Percentage of Solvents Pumped

46


|


Timer

A timer appears on the top left which tracks the time elapsed since the beginning of a ethod or purge in minutes and seconds . The timer is reset after each run .

Figure 64: Timer

Virtual Keyboard

Figure 65: Virtual Keyboard

Flow Rate Indicator

Provides the current flow rate of the system when it is running and the programmed flow rate set when the system is paused or stopped .

Figure 66: Flow Rate Indicator and Active Areas


|

User’s Guide

47

Pressure Indicator

Provides the current pressure levels while the system is running . The maximum pressure level can be adjusted via the keypad .

Figure 67: Adjusting the Maximum Pressure Level

Injection Valve

An image representing the injection valve and injection valve and its associated loop is provided . A separate indicator provides the current position of the injection valve . Users can manually switch the

6-way valve to load the loop with sample or inject the loop into the hydraulic circuit, according to its initial position .

Figure 68: Injection Valve and Active Areas

Backflush Valve

Two images show the backflush valve . An additional indicator provides the current position of the backflush valve . Red areas permit the manual switching of the 4-way backflush valve to pump to the column, either

Direct

or

Reverse

, according to its initial position .

Figure 69: Backflush Valve and Active Areas

48


|


Column Selection

Users can select the column or columns from a dropdown list by selecting the column . This feature is disabled when the method starts .

Figure 70: Column Selection and Active Areas

Column Switching

The synoptic screen also displays the current position of the column switching valve . Users can manually switch the 6-way valve to use columns

1

or

2

, according to its initial position .

Figure 71: Column Switching and Active Areas

Lamp Activation

A green or red dot indicates the state of the deuterium detector lamp .

● Green = ON

● Red = OFF

1

Figure 72: Lamp Switching and Active Areas

2


|

User’s Guide

49

Active Lamps

When the system is stopped or in

Purge Mode

. A dialog box allows users to switch the lamp on or off . The dialog box will appear in green or red, depending on its current state . After switching off, a counter indicates the necessary cooling period before reactivation .

Figure 73: Active Lamp and Cool Down Counter

Auto Zero Dialog Box

When the system is running . A dialog box will appear, asking users to

Auto Zero

, or reset the absorbance signal, of the detector .

Figure 74: Auto Zero Dialog Box

50


|


Wavelength Indicators

Two wavelength indicators showing the current adjustments of the detector(s) .

Figure 75: Wavelength Indicators

The following parameters can be changed, except when a method is started or in

Purge Mode

:

● The wavelength adjustment via the keypad by selecting

Value

● The adjustment of low and high wavelengths if

SCAN

mode is chosen

● Deactivation of the signal selecting

None

Figure 76: Active vs . Inactive Signal

The following parameters can be changed when a method is started:

● Signals can be made visible or invisible by selecting on the numbered green light next to the wavelength readout or by selecting

View > Signal 1 OR Signal 2

to check (activate) or uncheck

(deactivate) the wavelength

● The colored button next to the wavelength channel determines how the fraction collector is color coded in peak tracking .

Figure 77: Color Coding Peak Tracking


|

User’s Guide

51

3-Way Collection Valve

The 3-way collection valve permits the manual switching of flow to the collection tubes, waste, or back to its initial position .

Figure 78: 3-Way Collection Valve

Collection Tube Volume

The fraction collector (mL) shows the current volume in the collection tube when running, or the volume desired in every tube for the next collection when the system is paused or stopped . Maximum volume depends on the type of racks used .

Figure 79: Collection Tube Volume

Waste Volume

The waste container also displays its current volume . Users can confirm the emptying of the waste container by selecting

OK

when prompted by the dialog box .

Figure 80: Waste Volume and Confirmation

52


|


Peak Tracking

This panel shows the current status of fraction collection and helps users visualize the type of racks used

(code, tubes size, rack type and volume), their location on the tray, and the tubes already filled (partially or fully) . The rack set number is also displayed .

1 4

2

3

Figure 81: Peak Tracking Panel

Tubes have different colors, depending on if the

Peak

function is selected in the

View Menu

or not .

When a method is running or in

Purge Mode

:

● Active areas in shown in yellow (will not appear as such in the software) allow users to configure the racks used .

● Active areas shown in blue (will not appear as such in the software) allow users to validate changes to the racks or revert to the initial conditions with empty tubes .

When more than one rack set has been used:

● Active arrows shown in green (will not appear as such in the software) allow users to navigate to a different rack set .

When the

Direct Position Access

feature is activated:

● Active areas shows in purple (will not appear as such in the software) allow users to manually select the next collection point for any empty tube by double-clicking that tube .


|

User’s Guide

53


This panel tracks the details about the collection of the method in progress .

Figure 82: Collection Table

Selecting a tube on the

Peak Tracking Panel

highlights the peak on the


and visa versa .

The last line tracks the volume of liquid sent to

Waste

.

54


|


Curves Panel

This panel contains all the curves and chromatogram(s) that represent the results of a purification or a separation, which corresponds to the sequence of absorbance values sent by the UV detector at the given wavelength(s) during all the process . Fraction collection depends on the reference signal chosen .

Chromatogram

“Channel 1”

Ratio

“Ch. 1 & Ch. 2”

Chromatogram

“Channel 2”

Figure 83: Curves Panel

This panel can be used to edit methods graphically by moving curve points or segments . Active elements for the method appear on top of the

Curves Panel

, allowing users to

Manually Inject

,

Stop Modes

, and change the

Backflush Valve Position

.

Manual Injection

Stop Mode


|

User’s Guide

55

Backflush Valve Position (Direct)

Backflush Valve Position (Reverse)

● In addition to the curve parameters (

Flow Rate

,

%A

,

%B

,

%C

,

%D

,

Pressure

,

Threshold

, etc .) and chromatograms, users can view the sample name, and tubes used for collection (number and color) .

● During the execution of an

Equilibration Phase

, the panel background is grey, the signal plotted and the corresponding values are not stored, but are viewable for reference . Once the method is started, the background becomes white .

● If the

Peak

function is checked in the

View Menu

, the colors are different . Separations between two consecutive tubes are made by vertical black lines .

Figure 84: Peak Tracking Color Coding, Left (Unchecked) and Right (Checked)

● When the cursor is stationary over any part of the

Curves Panel

, an annotation appears . This yellow background frame includes all working parameters at the time where is placed the cursor (

Time

,

Signal 1

,

Signal 2

,

%A

…

%D

,

Flow Rate

, and

Pressure

) .

Figure 85: Hover Over Box with Working Parameters

● The scales are not always adapted to the absorbance values delivered by the detector or to the analysis time .

56


|


Y-Axis (UV Signal) = Absorbance

1 . Active areas in blue (will not appear as such in the software) allow users to modify the start and end values of the UV signal . Only effective if

Automatic Scale

is unchecked in the

Visualization Limit

Values

dialog box .

X-Axis = Time or Column Volume

2 . Active area shown in purple (allows for the modification of the

Elution Unit

used in the method and visualization of the results) . Users must select a cartridge to install before they can change these parameters .

1 2

Figure 86: Absorbance and Time Scale


|

User’s Guide

57

Vertical Toolbar

A six button vertical toolbar is anchored to the right side of the

Curves Panel

:

Zooms in or out when placed over a curve

Shows or hides the curves for the method displayed on the screen

Adds a new step (

Elution

or

Equilibration

) after the step currently selected

Deletes the selected step

Goes to the edition of

Equilibration

steps (panel background becomes grey)

Goes to the edition of

Elution

steps (panel background becomes white)

You can also select a part of the chart by sliding the cursor of the mouse to define a rectangle . The screen will resize to fit your selection . Double-click on the center of the

Curves Panel

to undo this action and view the entire

Curves Panel

.

58


|


File Management

Contents

Presentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Wizard Description: List of Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Creating Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Copying Methods and Sequences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Saving Methods and Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Deleting Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62


|

User’s Guide

59

Presentation

The

File Management

window allows users to manage the different files generated by the software .

The

File Management

window is a step-by-step wizard, which can be accessed by selecting

File > File Management .

Two standard buttons allow navigating in the dialog steps,

Next

to pass to the following step and

Back

to return to the preceding step . At the end,

Finish

completes the action done . At any time,

Cancel

closes the

File Management

window .

Another cursor at the bottom allows users to adjust the transparency of the window .

Wizard Description: List of Actions

Figure 88: File Management Wizard

As for others windows or dialog boxes, it includes at the bottom, a cursor that allows adjusting the transparency . The more the cursor is rightward, the more the window is opaque; the more the cursor is leftward, the more the window is transparent .

Creating Methods

Create a New Method

Create > Next > Create a New Method > Next

Create a New Sequence

Create > Next > Create a New Sequence... > Finish

60

File Management

|


Copying Methods and Sequences

Copy a Method from a Numbered Shortcut

Copy > Next > Copy a Method from a Numbered Shortcut > Next

Copy a Method from a Named File

Copy > Next > Copy a Method from a Named File > Next

Copy a Method from Stored Data

Copy > Next > Copy a Method from Stored Data > Next

Copy a Sequence

Copy > Next > Copy a Sequence > Finish

Saving Methods and Data

Save the Current Method as a Numbered Method

Save > Next > Save the Current Method > Next > Save as Numbered Method > Finish

Save the Current Method as a File Method

Save > Next > Save the Current Method > Next > Save as File Method > Finish

Save the Current Data

Save > Next > Save the Current Data > Finish


|

User’s Guide

61

Deleting Files

Deleting a Numbered Method

Delete > Next > Delete a Numbered Method > Finish

Deleting a File Method

Delete > Next > Delete a File Method > Finish

Deleting Data

Delete > Next > Delete Data > Finish

Deleting a Sequence

Delete > Next > Delete a Sequence > Finish

62

File Management

|


Methods

Contents

Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Creating Methods with the Graphical Interface . . . . . . . . . . . . . . . . . . . . 64

General Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

Settings Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Equilibration Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

Elution Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

Tabular & Graphical Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

Detection Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Collection Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Method Runs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Sequence Edition and Starting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

Wait Messages and Error Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90


|

User’s Guide

63

Methods

Creating Methods

Methods can be created with the tabular, graphical interface or with the ordered lists found in the

User

Profile

.

Graphical Interface Methods

File > File Management > Create > Next > Create a New Method

When users select the

Title

field, a


will appear . Users can either type the method name

(when using a computer) or enter the title manually on the


.

Figure 89: Virtual Keyboard

Method Panels

The method creation window is divided into six tabbed panels:

General

,

Settings

,

Equilibration

,

Elution

,

Detection

, and

Collection

.

Figure 90: Tabbed Method Panel

At any time, the

OK

button confirms the parameters set in the method (on any of the tabbed panels) and returns users to the main screen . The

Cancel

button closes the method creation window without saving the changes .

Selecting the

Run

button will trigger the currently edited method to run on demand .

64

Methods

|


General Panel

This panel allows users to change the general data of the method which will be displayed on the report and used for traceability:

Title

,

Author

,

Sample Name

,

Solvents Used

, and

Comments

.

Figure 91: General Panel

Eluents can be selected from a drop-down list or entered manually . Users can enable or disable each gradient by clicking on the green or red circle next to the name .

Figure 92: Active and Inactive Eluents


|

User’s Guide

65

Settings Panel

The

Settings Panel

defines the following parameters for the method:

Column Type

,

Injection Modes

,

Pressure

,

Stop Mode Options

,

Elution Unit Options

, and

Automatic Gradient Optimization

.

Figure 93: Settings Panel

Column

Selected from a drop-down menu from a list of columns the user can edit in the

Setup > Columns

menu .

By default, no column will be selected . If the system is equipped with an optional device to add additional columns, extra field/s will appear . After selecting the column, a dialog box will appear, prompting the users to enter a flow rate . Make sure that flow rates are consistent with the specifications defined by the column manufacturer .

Figure 94: Column Flow Rate Prompt

66

Methods

|


Stop Mode

Controls the behavior of the system at the end of a method, particularly the time of the last elution step and the actions performed . It is not related to the manual stop by the clicking of the

Stop

button on the

PLC 2050/2250/2500 Purification System

. By default, the

Stop Mode

is set to

Pause

. Select the button next to

Pause

,

Standby

,

Continue (ICM)

, or

Stop

to activate . Only one mode of the four can be selected .

The

Continue (ICM)

and

Stop

modes have the additional option to toggle the lamp switch on or off by selecting the button next to

Lamp Switch Off

.

Pause

The message,

Method Ended: Do You Wish to Continue?

appears .

Standby

When the method is finished, the message,

Method Ended: Do You Wish to Continue?

appears .

Yes

closes the message, and

No

stops the method .

Continue (ICM): Intelligent Continuous Mode

When the method is finished, the dialog box below appears .

Figure 95: ICM Dialog Box

The pump will continue to operate with the previous parameters for ten minutes (configurable in the

User Profile

window) to check signal activity (peak):

● If no activity is detected during these ten minutes, the method stops .

● If a signal is detected that is higher than or equal to the lowest signal activity detected during the method, the ICM restarts for an additional ten minutes .

Stop

When the last elution step is reached, the method stops . When the

Stop Mode

chosen is

Continue

or

Stop

, an automatic action becomes available at the end of the method . The green circle indicates that action is enabled while the red circle indicates that the action is disabled .


|

User’s Guide

67

Lamp Switch Off

Select the circle next to

Lamp Switch Off

to toggle between

Enabled (Green)

and

Disabled (Red)

.

Figure 96: Toggling the

Lamp Switch

Once the method stops, the dialog box below appears . A fifteen minute countdown begins before the lamp is automatically switched off . Users can manually switch off the lamp by selecting

Switch OFF

or by cancelling the process .

Figure 97: Lamp Switching

Off Message

Once the deuterium lamp is switched off, another dialog box appears to indicate the required cooling time .

Figure 98: UV Lamp Cooling

Max Pressure

Allows users to adjust the maximum pressure allowed during operation . If this value is exceeded, an error message is displayed . By default, the

Maximum Pressure

is set to

230 bar

; however, the value depends on the column used .

ELSD Temperature

Allows users to set the temperature for the evaporative light scattering detector (ELSD) .

68

Methods

|


Elution Unit

Allows users to toggle the unit displayed on the chromatogram between

Time

and

Column Volume

.

Automatic Gradient Optimization (AGO)

When the detector signal goes above the threshold set in the method (when the feature is active) an automatic hold gradient command occurs: gradients will be fixed and are not modifiable . When the signal goes below the threshold, an automatic resume command is executed: gradients are restarted and are again modifiable . The duration of the method is shifted to correspond to the duration of the peaks .

Manually selecting

Resume

during peak acquisition will momentarily deactivate the

AGO

function . The timeout of this function is modifiable in the

User Profile

menu:

User Profile > Advanced Settings >

Others

.

Injection Mode (Section 1)

Allows users to condition the column before injection with or without an equilibration phase .

Equilibration

By default, the value is set to

Equilibration

. Set the value to

None

to deactivate .

Injection Mode (Section 2)

Controls the injection process for samples and the way methods are started and data is acquired .

Auto (Default)

Preliminary injection of the sample is sent directly to the column . This process starts immediately after the equilibration phase .

Loop Injection

Preliminary loading of sample is performed with a syringe and diluted in solution in a 6-way valve port .

Wait for Injection

Injection of sample is sent directly to the column, but it has to be confimed manually by the user before the method starts . This option is only possible if an equilibration phase is previous performed . Users will be prompted to make the injection by the following dialog box . Clicking

Yes

starts the method and data acquisition . Equilibration duration can be decreased by selecting

Start

at any time during its execution .

Columns

Allows users to select the type of column used in the method .


|

User’s Guide

69

Equilibration Panel

This panel allows users to define the equilibration steps, either with a table or graphically . The graphic version of equilibration steps appears with a gray background to differentiate it from the elution panel .

The equilibration process is used to condition the column before sample injection . When active, the detector signal is acquired and the chromatogram is plotted to help the user visualize the development and the end of the equilibration . When the method ends, the curves are erased without being saved, then the injection is done . Otherwise, the equilibration phase can be manually stopped at any time by clicking the

Start

button on the toolbar .

Figure 99: Equilibration Panel (Graphical Version)

Figure 100: Equilibration Panel (Table Version)

70

Methods

|


Elution Panel

This panel allows users to define the elution steps, either with a table or graphically . The graphic version of elution steps appears with a white background to differentiate it from equilibration steps .

Figure 101: Elution Panel (Graphical Version)

Figure 102: Elution Panel (Table Version)


|

User’s Guide

71

Consumption of Solvents

The consumption of solvents is calculated in advance of the method run on top right corner of the elution panel . The label on the top left of the panel indicates the value of the parameter currently selected .

Figure 103: Consumption of Solvents

Available Parameters for Equilibration and Elution Gradients

N°

The step number .

Start and End (Step Mode)

The time span between elution steps, measured in minutes and seconds (min:sec) .

Time (Slope Mode)

Since the beginning of the method, measured in minutes and seconds (min:sec) .

Flow Rate

The pump flow rate, measured in mL/min, adjustable in

Setup > User Profile > Physical Units > Flow

.

%A, %B, %C, and %D

The percentages for eluents . Columns displayed in the table depend on the system configuration and selections made in the

General Panel

.

72

Methods

|


Gradient Nodes

Nodes in the gradient are marked with colored circles (and arrows if a backflush valve is used) that correspond to the values written in the

Time

or

CV

column . For each node, users can define the quantities of solvents pumped, flow rate, and initial time by dragging and dropping the node on the graph . A single click on the node adjusts values in increments of five, whereas a double-click allows adjustments in increments of one .

Figure 104: Adjusting Nodes (No Backflush)

Backflush Valve Position

To change the position of the backflush valve for a gradient node, users must click on the arrows and drag the cursor up or down:

● Up for

Reverse

● Down for

Direct

Figure 105: Adjusting Nodes with Backflush


|

User’s Guide

73

Curves Display

When the method is in progress, the part already executed is highlighted in yellow in the

Curves Panel

and the nodes passed will disappear . Method modifications are not allowed before this point; however, nodes in front of this point can be modified as the method runs or when it is paused . Curves become more distinct as time elapses, and the leading edge of the curve is marked with a bold line .

Figure 106: Curves Display

Zoom Out Tool

Clicking the

Zoom Out

button on the

Curves Panel

displays the full

Curves Panel

.

74

Methods

|


M agniFying tHe

C urVeS

p anel

Click and drag on the

Curves Panel

to zoom in . A dotted rectangle will appear in the area you want zoom in on . The

Curves Panel

will adjust itself to your selection .

Figure 107: Magnification of the Curves Panel

Show/Hide Gradient Button

The second button of the

Curves Panel

toolbar allows users to

Show

or

Hide

the plot of different gradients until the method ends . When it is unselected, the results visualization is less crowded but the interactive edition on main screen is not possible .

Figure 109: Gradient Nodes Hidden on Curves Panel

Figure 108: Gradient Nodes Shown on Curves Panel


|

User’s Guide

75

Add Step and Delete Step Buttons

When the method is active (running or paused), the

Add Step

and

Delete Step

buttons become available .

New steps will have the same parameters as the previous steps . Use the

Delete Step

button to remove selected nodes .

After each modification, the

Curves Panel

is immediately updated . Nodes can be manually dragged to a new position . Gradients automatically adjust to added or deleted nodes .

Show/Hide Cursor

Shows or hides a moveable cursor which displays the exact

Time

,

Eluent Composition

,

Flow Rate

, and

Pressure

for a particular cross-section of the curves panel .

Figure 110: Gradient Cross-Section Cursor

Equilibration and Elution Toggle Buttons

Clicking the

Left Arrow (Equilibration)

or the

Right Arrow (Elution)

toggles the

Curves Panel

between these two views .

76

Methods

|


Tabular & Graphical Interfaces

The

Equilibration

and

Elution Panels

utilize both graphical and tabular interfaces . Both panels feature: a

Slope / Step Toggle Button

,

Table / Graph Toggle Button

,

Add Node Button

,

Delete Node Button

, and

Incremental Addition and Subtraction Buttons

.

Slope vs. Step Toggle Button

Toggles between the slope and step gradients shown below .

Figure 111: Slope Mode

Figure 112: Step Mode


|

User’s Guide

77

Table vs. Graph Toggle Button

Figure 113: Tabular Mode

Figure 114: Graphical Mode

Add or Delete Node Buttons

Clicking on

Add

or

Delete

in the equilibration or elution panels creates a new node with adjustable parameters . In the graphical mode these adjustments are made by dragging-and-dropping the node .

In the tabular mode, these nodes are adjusted by typing or manually adjusting the parameters of the columns .

Incremental Addition and Subtraction Buttons

78

Clicking on

++

or

- -

will add or subtract values in units of ten .

Methods

|


Clicking on

+

or

-

will add or subtract values in units of one .

Detection Panel

This panel allows users to define the parameters related to signal detection . The panel contains four

Detection Channels

,

Add Step

and

Delete Step

buttons, a

Detector Selection Drop-Down Box

,

Run

button, and

Incremental Addition and Subtraction

buttons .

Figure 115: Detection Panel


|

User’s Guide

79

UV600 Detector Parameters

N°

Step number .

Time

Time since the start of the method (min/sec) .

WL (nm)

Wavelength of the detector channel selected first in the

Settings

panel . The available range depends on the model of detector used .

Collect

Select

Yes

or

No.

Threshold

Select a value between

-500 and 5000 mAU (Absorbance Unit)

. Collection begins when a signal crosses the threshold .

F1

Level of filtration for each detector channel between 1 and 10 . 1 = inactive filter . 10 = maximum filter .

80

Methods

|


UV600 Scan Detector Parameters

N°

Step number .

Scan<<


200

and

600

with the


.

>>Scan

Select a value between the

Highest Scan<< Value

and

600

with the


.

Collect

Select

Yes

or

No.

Threshold


-500

and

5000 mAU (Absorbance Unit)


F1

Level of filtration for each detector channel between

1

and

10

.

1 = Inactive Filter

.

10 = Maximum Filter

.


|

User’s Guide

81

ELSD

N°

Step number .

Time


Gain


1

and

12

.

Collect

Select

Yes

or

No.

Threshold


-500

and

5000 mAU (Absorbance Unit)


F1

Level of filtration for each detector channel between

1

and

10

.

1 = Inactive Filter. 10 = Maximum Filter

.

82

Methods

|


Collection Panel

This panel manages fraction collection during the method run . The following parameters are available on the collection panel:

Add Step

and

Delete Step

buttons,

N°

,

Time

,

Local

,

Volume

,

Recycling

,

Mode

,

Action

,

Incremental Addition

and

Incremental Subtraction

buttons,

Run

, and a graphical

Rack

Selection

window .

Figure 116: Collection Panel


|

User’s Guide

83

Add and Delete Step Buttons

Clicking

Add

or

Delete

creates or deletes a step in the collection process, e .g ., to switch to a threshold parameter or to send elutions to waste .

N°

Step number .

Time


Local

Indicates whether the

Local Minimum

is valid or not . When valid, the inflection of a curve (from a negative to positive slope) will trigger collection into a new tube .

Volume

The volume collected by the tube (mL) . Zero values are not permitted and appear as

Full

. The nominal volume of tubes are chosen in the

Settings

panel .

Mode

Sets the collection mode:

Threshold

,

All

, and

Waste.

Threshold

Determined by the value provided in

User Profile > Edit Method > Detection Panel > Collection

Threshold

or in the table in the

Detection Panel

.

All

Continuous flow into tubes . Regardless of the signal or threshold set, the volume collected is the value set in

User Profile > Advanced Settings > Collection > Tubes Filling Above/Below Threshold.

Waste

Continuous flow to waste, regardless of the absorbance signal .

84

Methods

|


Method Runs

Configure the System


comes configured for method runs; however, when using a computer, it is necessary to manually configure the system in

Setup > System Configuration

. Ensure that all bottles and waste containers reflect their actual values before starting a method .

Prepare the Containers

The software calculates the consumption of solvents and the volume rejected to waste . Current values for the waste and solvent bottles appear on the synoptic screen; however, if one of the containers is empty, too low, or full (waste), an error in the method may occur and trigger an alarm .

Fill and Empty Containers

To fill or empty a container, click on the bottle or waste container on the synoptic screen .

Figure 117: Bottle Fill Prompt

Figure 118: Waste Empty Prompt


|

User’s Guide

85

Prime the Pump

Priming fills the tubing to avoid introducing air bubbles into the hydraulic circuit . Air bubbles can disrupt the method by introducing phantom peaks in the chromatogram . Use the

Purge

function for the solvent bottle or injection pump of your choice .

Figure 119: Purge Button

Open a Method

Launches a drop-down box with the option to open

Current Method

,

Manual Method

,

Numbered

Method

,

File Method

, or

Sequence

.

Figure 120: Open Method Button

86

Methods

|


Run a Method

Select the

Run Button

from the

Edit Method

screen or the

Run Button

on the

Main Screen

.

Figure 121: Curves Panel during Active Method Run

Starting and Stopping a Method

When a method is running, use the toolbar buttons on the top of the

Main Screen

to

Pause

,

Hold

Gradient

,

Stop Method

,

Resume Method

(when paused), or

Start Method

(when stopped) . An error state or faulty operation, such as a pressure alarm, empty solvent bottles, full waste bottle, etc . causes the state to

Pause

.

Figure 122: Play, Pause, Hold Gradient, Resume, and Stop Buttons


|

User’s Guide

87

Sequence Edition and Starting

Create a New Sequence

Select the

File Management

button from the main screen, then

Create a New Sequence

.

88

S equenCe

o ptionS

Edit

Opens the method selected in the table for editing .

Add

Creates a new method at the end of the sequence . Select the

Method (.lfh file)

from Windows Explorer .

New

Creates a new method which can be inserted into the sequence .

Delete

Deletes the method selected on the table .

S equenCe

t able

o ptionS

The table, which is not directly editable, contains the following columns:

N°

Number of the method in the sequence .

Methods

|


Title

Title of the method, provided in the

General Panel

.

Sample

Sample name of the method, provided in the

General Panel

.

Racks

Number of racks used for the method, provided in

Settings Panel

.

Mode

Not used .

*Asterisk

Indicates that comments are attached to the field . Comments are displayed in the bottom frame and concern the use of columns or racks .

Silent Mode

Toggle the circular button between

Active (Green)

and

Inactive (Red)

to turn on or off the display boxes that appear at the end of a method to advise users to change racks .


|

User’s Guide

89

Wait Messages and Error Alarms

Wait Message

At times communication between the software and the instruments takes a few seconds . The message below may appear when a delay occurs .

Figure 123: Wait Message

Error Messages

Any error will trigger the method to

Pause

. Error messages are displayed in a dialog box . Users can select

OK

to confirm knowledge of the error and continue to run the method, if desired .

Figure 124: Error Message

90

Methods

|


Configurations

Contents

Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

User Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

Advanced Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

Menus Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

Curves Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

Edit Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

Reports Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

Physical Units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

System Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105


|

User’s Guide

91

Bottles

Accessed through

Setup > Bottles

. Users define the real volumes (mL) of the eluent containers and waste bottle . Capacities are defined, but contents are not . Adjust volumes with the

Up

or

Down

arrows or with the


.

Figure 125: Bottle Volume Window

92

Configurations

|


Columns

Accessed through

Setup > Columns

. The column table allows users to display and edit the following parameters:

Code

,

Name

,

Nature

,

Weight

,

Flow Rate

,

Pressure

,

Volume

,

Equil.

,

Bed Height

,

Trademark

, and

Bar Code

.

Figure 126: Column Editing Window

Add

Add a new column to the table .

Delete

Delete a column from the table .

Load

Load a

Column File (.csv)

into the table .

Clean-Up

Allows users to remove all columns loaded from a database, but not the ones created by users .

Import

Allows users to import a

Column List File (.cart)

.

Export

Allows users to save the

Column List

as a

(.cart)

file .


|

User’s Guide

93

Racks

Accessed with

Setup > Racks

. The following parameters are available in the racks list:

Code

,

Name

,

Title

,

Volume

,

Nb Rack X

(Number of Racks on the X Axis),

Nb Rack Y

(Number of Racks on the Y Axis),

Width (X)

,

Length (Y)

,

Offset X

,

Offset Y

,

Spacing X

,

Spacing Y

,

Offset RY

, and

Bar Code

. The

Load

button is used to transfer the table data to the collector and is saved as an (

.ini

) file .

Offset Diagram and Legend

OFFSET X = 1

OFFSET Y = 2

SPACING X = 3

SPACING Y = 4

SPACING R = 5 - 3

Position 0

2

1

5

4

3

Figure 127: Racks Lists

Figure 128: Offset Diagram

94

Configurations

|


Solvents

Accessed with

Setup > Solvents

. Users define the solvents used by the system by typing their name and bar code, which requires a

Bar Code Reader

. Users may

Add

or

Delete

solvents or edit the names with the


. Users may edit the solvent

Name

,

Strength

, or

Bar Code

.

Figure 129: Solvents Window


|

User’s Guide

95

User Profile

Accessed with

Setup > User Profile

. Users that have logged in will see their settings saved . A click on

+

or

-

will show or hide menus or submenus .

OK

confirms modifications and returns users to the main screen .

Cancel

closes the window without saving changes .

Restore

allows users to select settings from a dropdown list .

Save

opens Windows Explorer where users can save the

User Profile

as an (

.ini

) file .

User Profile Menus

●

User Interface

●

Advanced Settings

●

Menus Display

●

Curves Display

●

Edit Method

●

Reports Components

●

Physical Units


96

Configurations

|


Line Widths

Adjustable with the


or by clicking the

Up

or

Down

arrow .

Line Color

Adjustable with a preset color swatches or with customized RGB values when

Define Custom Colors

is selected .

Figure 131: Color Palette


|

User’s Guide

97

User Interface

Signals (1, 2, 3, and 4)

Signal Ratio

Eluents (A, B, C, and D)

Pressure

Flow Rate

Collection Threshold

Tubes

Peaks

98

Configurations

|


Advanced Settings

Paths

Collection

Detector Lamp

Bottles

Others


|

User’s Guide

99

Menus Display

100

Configurations

|


Curves Display

Elution Gradient

Pressure

Flow Rate

Tubes/Peaks

Signal (1, 2, 3, and 4)

Signal Ratio

Collection Threshold


|

User’s Guide

101

Edit Method

General & Elution Panels

Settings Panel

102

Configurations

|


Detection Panel

Collection Panel


|

User’s Guide

103

Reports Components

Physical Units

104

Configurations

|



Accessible through

Setup > System Configuration

. Users can select the types of instruments used in the system, their characteristics, and how the communication ports are arranged . Selecting the

OK

button confirms all modifications, whereas

Cancel

closes the dialog box without saving changes .

Figure 132: System Configuration Screen


|

User’s Guide

105

General Panel

Figure 133: General Panel

General

Users select

SPOT PREP

or

PC Stand-Alone

.

Detector

Four available slots with the following options:

● KNAUER K2001, K2501, S2500, or K2600

● ECOM LCD 2073A

● ECOM SAPPHIRE 600

● ECOM DAD 400, 600, or 800

● ECOM 06 SINGLE or DUAL

● ANALOG INPUT (Internal)

● USB-1408FS

● SEDEX 80LT (ELSD) or (ELSD+SAGA)

● KNAUER RI S2400

● KNAUER DAD S2600

● KNAUER K2600 (KNET)

106

Configurations

|


Pump Panel

Figure 134: Pump Panel

Pump

Users select

None

,

ARMEN V1

,

ARMEN V2

, or

KNAUER K1800 (KNET)

COM Selection

Users select the

Search

button, then select the

COM

port from the drop-down menu .

Maximum Flow Rate (0–5000 mL/min)

Minimum Pressure (0–300 bar)

Maximum Pressure (0–300 bar)

Adjustable with the virtual keyboard or

Up

or

Down

arrows .

Solvent Configuration Drop-Down Box

Users select

A

,

A+B

,

A+B+C

, or

A+B+C+D

.

Recycling Valve

High Pressure Gradient

Toggle the circular button to

Turn On (Green)

or

Turn Off (Red)

the recycling valve .


|

User’s Guide

107

Detector Panels (#1, #2, #3, etc.)

Figure 135: Detector Panels

Comm Port

Select the communications port from a drop-down box menu .

Search

Select

Click Here

to automatically locate the detector .

Wavelength Minimum (0–1000 nm)

Wavelength Maximum (0–1000 nm)

Delay Volume (0–100 mL)

Adjustable with the


or

Up

or

Down

arrows .

108

Configurations

|


Collector Panel

Figure 136: Collector Panels

1

St

C olleCtor

Type

Users can select the following from the drop-down menu:

● ARMEN 4S, 3L, or LS5600

● GILSON FC204 or FC203

● ISCO FOXY 200

● LABOCOL VARIO 2000

● MULTIVALVES

● MULTIVALVES II

Comm Port


Search

Select

Click Here


Dead Volume (0–99 mL)

X Origin (0–999 mm)

Y Origin (0–999 mm)

Adjustable with the


or

Up

or

Down

arrows .


|

User’s Guide

109

2 nd

C olleCtor

Tube Pattern

Rack Pattern

Select

Alternating Vertical

,

Continuous Vertical

,

Continuous Horizontal

, or

Alternating Horizontal

.

Horizontal (X)

Vertical (Y)

Select

Left

or

Right

.

110

Configurations

|


Valves Panel

Figure 137: Valves Panels

Select

Yes

or

No

for the parameters below . Additional or alternative options will be given, if applicable, or users will have to type in a desired value .

V alVe

i njeCtion

(l oop

)

Type

Select

Standard (PUMP)

,

Via CPC

,

VICI RS485

,

VICI RS232

, or

GSIOC

.

Comm Port


Address

Adjustable with the


.

Search

Select

Click Here


Reverse

Select

Normal

or

Reverse

.


|

User’s Guide

111

b aCk

F luSH

V alVe

Type

Select

Standard (PUMP)

,

Via CPC

,

VICI RS485

, or

VICI RS232

.

Comm Port


Address

Adjustable with the


.

Search

Select

Click Here


Reverse

Select

Normal

or

Reverse

.

C oluMn

S witCH

Select

NO COLUMN SWITCH

,

SWITCH TO COLUMN 1

,

SWITCH TO COLUMN 2

, or

SWITCH BY METHOD

.

Type

Select

Standard (PUMP)

,

VICI RS485

, or

VICI RS232

.

Comm Port


Address

Adjustable with the


.

Search

Select

Click Here


Reverse

Select

Normal

or

Reverse

.

112

Configurations

|


i njeCtion

S top

V alVe

Type

Select

Via Inj. Pump

,

VICI RS485

, or

VICI RS232

.

Comm Port


Address

Adjustable with the


.

Search

Select

Click Here


Reverse

Select

Normal

or

Reverse

.


|

User’s Guide

113

Injection Panel

Figure 138: Injection Panel

i njeCtion

V ia

p uMp


Turn On (Green)

or

Turn Off (Red)

injection .

Select

Main Pump

or

External Pump

by selecting the circular button next to the text .

Pump

Select

NONE

,

ARMEN V1

,

ARMEN V2

, or

KNAUER K1800 (KNET)

.

CoM S eleCtion

Users select the

Search

button, then select the

COM

port from the drop-down menu .

M axiMuM

p reSSure

(0–300 bar

)

Adjustable with the


or

Up

or

Down

arrows .

114

Configurations

|


Alarms Panel

Figure 139: Alarms Panel

S olVent

l eVel

a larMS


Turn On (Green)

or

Turn Off (Red)

the solvent level alarms .

d eteCted

l eVel

a larMS


Turn On (Green)

or

Turn Off (Red)

the detected level alarms, then select either

Global Signal

or

Separate Signals

.


|

User’s Guide

115

Gilson Glider Prep Software User's Manual

Gilson Glider Prep Software

User’s Guide

Table of Contents

Introduction

Contents

Overview

System Options

User Interface

File Menu

Open Method Button

Open Data Button

Setup Menu

Save Method Button

Control Menu

View Menu

Log Menu

Help Menu (?)

Interface Organization

Contents

Overview

Synoptic Screen

Peak Tracking

Collection Table

Curves Panel

Vertical Toolbar

File Management

Contents

Presentation

Wizard Description: List of Actions

Creating Methods

Copying Methods and Sequences

Saving Methods and Data

Deleting Files

Methods

Contents

Methods

Graphical Interface Methods

General Panel

Settings Panel

Equilibration Panel

Elution Panel

Tabular & Graphical Interfaces

Detection Panel

Collection Panel

Method Runs

Sequence Edition and Starting

Wait Messages and Error Alarms

Configurations

Contents

Bottles

Columns

Racks

Solvents

User Profile

User Interface

Advanced Settings

Menus Display

Curves Display

Edit Method

Reports Components

Physical Units

System Configuration

Related manuals

Gilson

PLC 2500

Film-Tech

IREM EX-100GM3-E

Gilson

VERITY 1910 MS

Gilson

CPC 250 PRO

Table of contents